CO2018007568A2 - Composiciones y métodos para el direccionamiento eficaz de transgenes - Google Patents

Composiciones y métodos para el direccionamiento eficaz de transgenes

Info

Publication number
CO2018007568A2
CO2018007568A2 CONC2018/0007568A CO2018007568A CO2018007568A2 CO 2018007568 A2 CO2018007568 A2 CO 2018007568A2 CO 2018007568 A CO2018007568 A CO 2018007568A CO 2018007568 A2 CO2018007568 A2 CO 2018007568A2
Authority
CO
Colombia
Prior art keywords
methods
plants
addressing
compositions
efficient transgene
Prior art date
Application number
CONC2018/0007568A
Other languages
English (en)
Inventor
Aihua Shao
Christine M Ellis
Michael E Goley
Clayton T Larue
Sherry L Leclere
Qungang Qi
Kwan Y Thai
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of CO2018007568A2 publication Critical patent/CO2018007568A2/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
    • C12Y103/03004—Protoporphyrinogen oxidase (1.3.3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invención provee moléculas de ADN recombinante y construcciones útiles para proveer localización subcelular de transgén eficaz de proteínas en plantas transgénicas. También se provén moléculas de ADN recombinante y construcciones para conferir tolerancia o resistencia a herbicidas a plantas, así como plantas que presentan tolerancia a herbicidas y métodos para producir o utilizar dichas plantas.
CONC2018/0007568A 2015-12-21 2018-07-19 Composiciones y métodos para el direccionamiento eficaz de transgenes CO2018007568A2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562270180P 2015-12-21 2015-12-21
US201662364715P 2016-07-20 2016-07-20
PCT/US2016/067531 WO2017112589A1 (en) 2015-12-21 2016-12-19 Compositions and methods for efficient targeting of transgenes

Publications (1)

Publication Number Publication Date
CO2018007568A2 true CO2018007568A2 (es) 2018-07-31

Family

ID=59065936

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2018/0007568A CO2018007568A2 (es) 2015-12-21 2018-07-19 Composiciones y métodos para el direccionamiento eficaz de transgenes

Country Status (25)

Country Link
US (1) US10563220B2 (es)
EP (2) EP3394272B1 (es)
JP (2) JP6961611B2 (es)
KR (1) KR20180089518A (es)
CN (2) CN116355928A (es)
AU (2) AU2016377487B2 (es)
BR (1) BR102016029899A2 (es)
CA (1) CA2950803A1 (es)
CO (1) CO2018007568A2 (es)
DK (1) DK3394272T3 (es)
EC (1) ECSP18054745A (es)
ES (1) ES2941913T3 (es)
HR (1) HRP20230311T1 (es)
HU (1) HUE061632T2 (es)
MX (1) MX2018007705A (es)
PE (2) PE20181540A1 (es)
PH (1) PH12018501317A1 (es)
PL (1) PL3394272T3 (es)
PT (1) PT3394272T (es)
RS (1) RS64091B1 (es)
RU (1) RU2751238C2 (es)
UA (1) UA126554C2 (es)
UY (1) UY37040A (es)
WO (1) WO2017112589A1 (es)
ZA (1) ZA201803723B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108235709B (zh) 2015-08-03 2023-09-29 孟山都技术公司 用于植物的除草剂耐受性的方法和组合物
US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
AU2017301976B2 (en) * 2016-07-29 2022-04-07 Basf Se Method for controlling PPO resistant weeds
KR102587506B1 (ko) 2016-07-29 2023-10-11 몬산토 테크놀로지 엘엘씨 식물에서 유전자 발현을 위한 방법 및 조성물
CA3026528A1 (en) 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance
US11758907B2 (en) 2017-12-20 2023-09-19 Sumitomo Chemical Company, Limited Method for controlling harmful organisms in crops
CA3057917A1 (en) * 2018-10-16 2020-04-16 Monsanto Technology Llc Brassica event mon94100 and methods of use thereof
WO2023044364A1 (en) 2021-09-15 2023-03-23 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors
WO2023137309A2 (en) 2022-01-14 2023-07-20 Enko Chem, Inc. Protoporphyrinogen oxidase inhibitors

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US10378023B2 (en) 2015-09-01 2019-08-13 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
KR102587506B1 (ko) 2016-07-29 2023-10-11 몬산토 테크놀로지 엘엘씨 식물에서 유전자 발현을 위한 방법 및 조성물
CA3026528A1 (en) 2017-12-15 2019-06-15 Monsanto Technology Llc Methods and compositions for ppo herbicide tolerance

Also Published As

Publication number Publication date
RU2751238C2 (ru) 2021-07-12
EP4219729A3 (en) 2023-09-27
CN108463554B (zh) 2023-04-18
KR20180089518A (ko) 2018-08-08
AU2020281170A1 (en) 2021-02-04
PH12018501317A1 (en) 2019-02-18
EP3394272B1 (en) 2023-01-11
ZA201803723B (en) 2019-07-31
PE20181540A1 (es) 2018-09-26
RS64091B1 (sr) 2023-04-28
AU2016377487B2 (en) 2020-12-24
ES2941913T3 (es) 2023-05-26
BR102016029899A2 (pt) 2017-06-27
JP2022009102A (ja) 2022-01-14
PE20240226A1 (es) 2024-02-16
US10563220B2 (en) 2020-02-18
CN108463554A (zh) 2018-08-28
HUE061632T2 (hu) 2023-07-28
EP3394272A4 (en) 2019-07-31
RU2018124597A3 (es) 2020-05-28
EP4219729A2 (en) 2023-08-02
UY37040A (es) 2017-07-31
DK3394272T3 (da) 2023-04-03
PL3394272T3 (pl) 2023-05-08
HRP20230311T1 (hr) 2023-05-12
CA2950803A1 (en) 2017-06-21
JP7312805B2 (ja) 2023-07-21
RU2018124597A (ru) 2020-01-23
JP2019500060A (ja) 2019-01-10
CN116355928A (zh) 2023-06-30
PT3394272T (pt) 2023-04-11
AU2016377487A1 (en) 2018-06-28
AU2020281170C1 (en) 2021-11-18
AU2020281170B2 (en) 2021-05-20
WO2017112589A1 (en) 2017-06-29
EP3394272A1 (en) 2018-10-31
UA126554C2 (uk) 2022-11-02
ECSP18054745A (es) 2018-08-31
JP6961611B2 (ja) 2021-11-05
MX2018007705A (es) 2018-11-09
US20170175131A1 (en) 2017-06-22

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