CN87209860U - Single cell separator - Google Patents
Single cell separator Download PDFInfo
- Publication number
- CN87209860U CN87209860U CN 87209860 CN87209860U CN87209860U CN 87209860 U CN87209860 U CN 87209860U CN 87209860 CN87209860 CN 87209860 CN 87209860 U CN87209860 U CN 87209860U CN 87209860 U CN87209860 U CN 87209860U
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- CN
- China
- Prior art keywords
- pump
- mesh screen
- sieve
- piston
- single cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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Abstract
The utility model relates to a separating device for the histiocytic cells of animals and plants, comprising a sieve pump, a pump and a piston which is matched with the pump. The inner diametre (diameter) of the pump is equal to the diameter of the maximum cross section of the working part of the sieve, the shape of the piston complements the shape of the contact part of which the piston contacts to the sieve. The optimization technical indicator of the utility model is that the specification of the sieve is 200 meshes, and the ratio of the tissue dose (mg) in one treatment and the operating area of the sieve (square millimeter) is 2; 1. The practice shows that the utility model has the advantages of simple structure, convenient operation, saving time and high separating rate of single cell, the separated material is easy to be maintained sterile, and the single cell separator is an ideal single cell separator.
Description
The utility model is animal vegetable tissue cell separation apparatus (B01D)
Existing single-cell technique is mainly two kinds of enzyme digestion and shearing methods, narrow application range, and unicellular separation rate is low, operates cumbersomely, time-consuming, and isolate is easily contaminated.
The purpose of this utility model provides a kind of histocyte tripping device, and it can effectively improve unicellular velocity of separation.
The utility model is by mesh screen (2), and pump (4) and the piston (5) that adapts are with it formed.The specification of mesh screen is 100～400 orders; Pump internal diameter (diameter) is identical with the maximum cross section diameter of mesh screen working portion, and the shape of piston and mesh screen contact part is coincide.Primary treatment tissue mass (milligram) is 0.5～3:1 with the ratio of mesh screen working area (square millimeter).
Accompanying drawing is unicellular separator synoptic diagram.
During operation, nut (1), screw (3) are fixed in pump (4) front end with mesh screen (2), put an amount of soft tissue in pump, overlap upper piston (5) then, promote piston handle (6) forward and left rotation and right rotation, soft tissue is separated into unicellular and cell mass minute quantity promptly by mesh screen, both can obtain pure unicellular after the filtration.Its best-of-breed technology index, mesh screen specification are 200 orders; Primary treatment tissue mass (milligram) is 2:1 with the ratio of mesh screen working area (square millimeter).
Facts have proved: the unicellular separation rate height of the utility model, simple in structure, easy to operate, save time, easily keep separator aseptic, the unicellular separation that is particularly useful for spleen, thymus gland, liver, kidney, lung and contains the less tumor tissues of fibroblast.
Claims (4)
1, a kind of tripping device that is used for the animal vegetable tissue cell, the piston composition that it is characterized in that mesh screen, pump and adapt with it.
2, tripping device according to claim 1 is characterized in that pump internal diameter (diameter) is identical with the maximum cross section diameter of mesh screen working portion, and the shape of piston and mesh screen contact part is coincide.
3, tripping device according to claim 1, the specification that it is characterized in that mesh screen is 100～400 orders; Primary treatment tissue mass (milligram) is 0.5～3:1 with the ratio of mesh screen working area (square millimeter).
4, tripping device according to claim 1, its best-of-breed technology index: the specification of mesh screen is 200 orders, primary treatment tissue mass (milligram) is 2:1 with the ratio of mesh screen working area (square millimeter).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87209860 CN87209860U (en) | 1987-07-08 | 1987-07-08 | Single cell separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87209860 CN87209860U (en) | 1987-07-08 | 1987-07-08 | Single cell separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN87209860U true CN87209860U (en) | 1988-05-25 |
Family
ID=4824881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 87209860 Withdrawn CN87209860U (en) | 1987-07-08 | 1987-07-08 | Single cell separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN87209860U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103343086A (en) * | 2013-06-28 | 2013-10-09 | 科晶（宁波）生物科技有限公司 | Integrated system for biological tissue treatment and cell collection |
| CN103468568A (en) * | 2013-09-16 | 2013-12-25 | 益善生物技术股份有限公司 | Filtering device, filtering piece and filtering method |
| CN104130936A (en) * | 2014-08-14 | 2014-11-05 | 江苏瑞明生物科技有限公司 | Oscillation type single cell separator |
| CN112625863A (en) * | 2020-12-30 | 2021-04-09 | 武汉北度生物科技有限公司 | Cell separator based on extract anti-oxidant active ingredient |
-
1987
- 1987-07-08 CN CN 87209860 patent/CN87209860U/en not_active Withdrawn
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103343086A (en) * | 2013-06-28 | 2013-10-09 | 科晶（宁波）生物科技有限公司 | Integrated system for biological tissue treatment and cell collection |
| CN103343086B (en) * | 2013-06-28 | 2016-02-03 | 科晶（宁波）生物科技有限公司 | A kind of integrated system for tissue processing and cell harvesting |
| CN103468568A (en) * | 2013-09-16 | 2013-12-25 | 益善生物技术股份有限公司 | Filtering device, filtering piece and filtering method |
| CN104130936A (en) * | 2014-08-14 | 2014-11-05 | 江苏瑞明生物科技有限公司 | Oscillation type single cell separator |
| CN104130936B (en) * | 2014-08-14 | 2016-01-27 | 江苏瑞明生物科技有限公司 | Oscillation mode single cell separator |
| CN112625863A (en) * | 2020-12-30 | 2021-04-09 | 武汉北度生物科技有限公司 | Cell separator based on extract anti-oxidant active ingredient |
| CN112625863B (en) * | 2020-12-30 | 2022-06-07 | 武汉北度生物科技有限公司 | Cell separator based on extract anti-oxidant active ingredient |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |