CN2793726Y - Co-focusing laser induced fluorescent detector of high-efficient liquid-phase chromatographic system - Google Patents
Co-focusing laser induced fluorescent detector of high-efficient liquid-phase chromatographic system Download PDFInfo
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- CN2793726Y CN2793726Y CN 200520091024 CN200520091024U CN2793726Y CN 2793726 Y CN2793726 Y CN 2793726Y CN 200520091024 CN200520091024 CN 200520091024 CN 200520091024 U CN200520091024 U CN 200520091024U CN 2793726 Y CN2793726 Y CN 2793726Y
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- phase chromatographic
- chromatographic system
- detection cell
- laser induced
- liquid phase
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a detector of a high-efficiency liquid-phase chromatographic system, particularly a co-focusing laser induced fluorescent detector of a high-efficient liquid-phase chromatographic system, which is provided with a new detecting cell. The utility model comprises an excitation light source, an optical platform and the detecting cell, wherein the excitation light source is positioned on one side of the optical platform on which the detecting cell is fixed; an object lens, a beam splitter mirror, a light filter, a convex lens and a light diaphragm are coaxially and orderly arranged in the optical platform from top to bottom, a photoelectric converting device is arranged on the lower part of the light diaphragm, wherein the beam splitter mirror and parallel light beams emitted by the excitation light source form an angle of 45DEG; two liquid flow pipelines are arranged in the detecting cell, detecting windows are arranged below the two liquid flow pipelines, stainless steel pads are pressed on the detecting windows, and a positioning hole and a fixed hole are also arranged on the detecting cell; the detecting cell is a detecting cell behind a column, and the two liquid flow pipelines and an excitation light path are on a same plane and mutually form 120DEG. The utility model has the advantages of compact structure, small size, high fluorescence collecting efficiency, etc.
Description
Technical field
The utility model relates to the detecting device of highly effective liquid phase chromatographic system, and particularly a kind of highly effective liquid phase chromatographic system that disposes novel detection cell is total to the focus type laser induced fluorescence detector.
Background technology
Fluoroscopic examination is used more extensive in high performance liquid chromatography (HPLC) system as a kind of highly sensitive detection technique.To adapt to different samples, the lowest detectable limit of this technology is generally 10 by selective excitation or emission wavelength
-8～10
-9Mol.Along with the development of science and technology and society, the requirement to the trace components analysis in present biotechnology, environment and the food reaches 10 sometimes
-12Mol even lower, traditional fluorescence detector can not meet the demands.With laser is that (Laser-Induced FluorescenceDetection is one of the highest detection means of present sensitivity LIF-D), has obtained fast development in recent years for the laser-Induced Fluorescence Detection of excitation source.Focus type LIF-D has simple in structure, phosphor collection efficient advantages of higher altogether, in Capillary Electrophoresis detects, use very extensive, but be applied to the HPLC system, need the special detection cell of design, detection cell comprises windowless pond, jet pond, sheath flow pool behind the post of report at present.Fluorescence detection cell based on detection cell behind these posts all is based on the orthogonal type optical texture except that sheath flow pool, although there is the report sheath flow pool to be used for the burnt optical texture of copolymerization, sheath flow pool complex manufacturing process, operation are loaded down with trivial details.
The utility model content
The purpose of this utility model is to provide a kind of highly effective liquid phase chromatographic system focus type laser induced fluorescence detector altogether.This detecting device by detection cell on the post or post after detection cell can with capillary liquid chromatography and common highly effective liquid phase chromatographic system on-line coupling, be applicable to that mainly the trace components in the fields such as biology, environment, medicine and food inspection detects.
The purpose of this utility model is achieved through the following technical solutions:
The utility model comprises excitation source, optical table and detection cell, and excitation source is positioned at a side of optical table, and detection cell is installed on the optical table.
Wherein: coaxial from top to bottom object lens, beam splitter, optical filter, convex lens and the diaphragm of being provided with successively in the described optical table, photoelectric conversion device is arranged on the below of diaphragm, wherein beam splitter and the excitation source parallel beam angle at 45 of sending; Described photoelectric conversion device can be photomultiplier, photoelectric cell, photodiode or charge-coupled image sensor; Described excitation source can be semiconductor laser, diode pumped solid state laser or gas laser; Be provided with two fluid pipes in the described detection cell, two fluid pipes below is provided with detection window, and stainless steel gasket is pressed on the detection window, also has pilot hole and fixed orifice on detection cell; Described detection cell is a detection cell behind the post, and two fluid pipes and excitation light path are on same plane and be in 120 °.
Advantage of the present utility model and good effect are:
The utility model is used for high performance liquid chromatography, has compact conformation, little, the phosphor collection efficient advantages of higher of volume.Simultaneously, the optical window of detection cell is the plane optical window behind the post of being developed, and is that detection cell has lower optical reflection and scattering on the capillary column of column type with respect to xsect, and promptly the optics background is little, helps obtaining lower detection sensitivity.
Description of drawings
Fig. 1 is an one-piece construction synoptic diagram of the present utility model;
Fig. 2 is the structural representation of detection cell behind the utility model post;
Fig. 3 is the chromatogram of the utility model laser-Induced Fluorescence Detection lactochrome.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
As shown in Figure 1, the present invention adopts phosphor collection light path and excitation light path at the homonymy of detection cell 9 and the coaxial burnt optical texture of copolymerization.Comprise excitation source 1, optical table 2 and detection cell 9, excitation source 1 is positioned at a side of optical table 2, and detection cell 9 is installed on the optical table 2.Coaxial from top to bottom object lens 8, beam splitter 3, optical filter 4, convex lens 5 and the diaphragm 6 of being provided with successively in the optical table 2, photoelectric conversion device 7 is arranged on the below of diaphragm 6, wherein beam splitter 3 and the excitation source 1 parallel beam angle at 45 of sending.Photoelectric conversion device 7 can be photomultiplier (PMT), photoelectric cell, photodiode or charge-coupled device (CCD).Excitation source 1 can be semiconductor laser, diode pumped solid state laser or gas laser.
As shown in Figure 2, be provided with two fluid pipes 11 in the detection cell 9, two fluid pipes, 11 belows are provided with detection window 10, stainless steel gasket 12 is pressed on the detection window 10, on detection cell 9, also have pilot hole 13 and fixed orifice 14, detection cell 9 is positioned on the optical table 2 by pilot hole 13, and is fixed on the optical table 2 by fixed orifice 14.Detection cell 9 is a detection cell behind the post, and two fluid pipes 11 and excitation light path are on same plane and be in 120 °, and the material of detection cell 9 is stainless steel or polyetheretherketone inert materials such as (PEEK).This detection cell 9 mainly is applicable to HPLC system and Flow Injection Analysis system.
When detecting, the parallel beam that excitation source 1 sends is at 45 to shine on the beam splitter 3, is reflected, focuses in the detection cell 9 through object lens 8, detection window 10.When flow of liquid was crossed fluid pipe 11, if in the sample fluorescence is arranged, after the light path that the is excited irradiation, the fluorescence that sample sends was collected, is focused on by convex lens 5 by object lens 8, and being converted into through diaphragm 6 to photoelectric conversion device 7 can be for the electric signal that detects.
As shown in Figure 3, be used to detect lactochrome for the utility model.
Test condition: HPLC system: one of liquid chromatography high pressure pump, separating column are 4.6 * 150mm C
18, 5 μ, (ZORBAX, HP); Moving phase: water/methyl alcohol (70/30).Detection cell behind the post: pond volume 7 μ L.
Test result: getting signal to noise ratio (S/N ratio) is 3, and this LIF-D is limited to 0.1 μ g/L to the concentration detection of lactochrome.
Claims (6)
1. a highly effective liquid phase chromatographic system is total to the focus type laser induced fluorescence detector, it is characterized in that: comprise excitation source (1), optical table (2) and detection cell (9), excitation source (1) is positioned at a side of optical table (2), and detection cell (9) is installed on the optical table (2).
2. be total to the focus type laser induced fluorescence detector by the described highly effective liquid phase chromatographic system of claim 1, it is characterized in that: coaxial from top to bottom object lens (8), beam splitter (3), optical filter (4), convex lens (5) and the diaphragm (6) of being provided with successively in the described optical table (2), photoelectric conversion device (7) is arranged on the below of diaphragm (6), wherein beam splitter (3) and excitation source (1) the parallel beam angle at 45 of sending.
3. be total to the focus type laser induced fluorescence detector by the described highly effective liquid phase chromatographic system of claim 2, it is characterized in that: described photoelectric conversion device (7) can be photomultiplier, photoelectric cell, photodiode or charge-coupled image sensor.
4. be total to the focus type laser induced fluorescence detector by claim 1 or 2 described highly effective liquid phase chromatographic systems, it is characterized in that: described excitation source (1) can be semiconductor laser, diode pumped solid state laser or gas laser.
5. be total to the focus type laser induced fluorescence detector by the described highly effective liquid phase chromatographic system of claim 1, it is characterized in that: be provided with two fluid pipes (11) in the described detection cell (9), two fluid pipes (11) below is provided with detection window (10), stainless steel gasket (12) is pressed on the detection window (10), also has pilot hole (13) and fixed orifice (14) on detection cell.
6. be total to the focus type laser induced fluorescence detector by the described highly effective liquid phase chromatographic system of claim 5, it is characterized in that: described detection cell (9) is detection cell behind the post, and two fluid pipes (11) and excitation light path are on same plane and be in 120 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520091024 CN2793726Y (en) | 2005-05-27 | 2005-05-27 | Co-focusing laser induced fluorescent detector of high-efficient liquid-phase chromatographic system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520091024 CN2793726Y (en) | 2005-05-27 | 2005-05-27 | Co-focusing laser induced fluorescent detector of high-efficient liquid-phase chromatographic system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2793726Y true CN2793726Y (en) | 2006-07-05 |
Family
ID=36820869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520091024 Expired - Fee Related CN2793726Y (en) | 2005-05-27 | 2005-05-27 | Co-focusing laser induced fluorescent detector of high-efficient liquid-phase chromatographic system |
Country Status (1)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614652B (en) * | 2008-06-25 | 2011-04-20 | 中国科学院大连化学物理研究所 | Self-aligned optical-fiber fluorescence detection cell and array fluorescence detection cell |
| CN102841075A (en) * | 2011-11-15 | 2012-12-26 | 中国科学院光电研究院 | Laser spectroscopy induced component detection system |
| CN104535290A (en) * | 2014-12-30 | 2015-04-22 | 黄真理 | Laser-induced fluorescence three-dimensional fluid detection system and method |
| CN109884061A (en) * | 2018-12-19 | 2019-06-14 | 长春理工大学 | Utilize the method for common focus point migration microscopic system measuring medium surface roughness |
| CN109884020A (en) * | 2018-12-19 | 2019-06-14 | 长春理工大学 | Using common focus point migration microscopic system to the non-destructive measuring method of micro/nano level Medium Wave Guide or stepped ramp type structure side wall angle |
-
2005
- 2005-05-27 CN CN 200520091024 patent/CN2793726Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614652B (en) * | 2008-06-25 | 2011-04-20 | 中国科学院大连化学物理研究所 | Self-aligned optical-fiber fluorescence detection cell and array fluorescence detection cell |
| CN102841075A (en) * | 2011-11-15 | 2012-12-26 | 中国科学院光电研究院 | Laser spectroscopy induced component detection system |
| CN102841075B (en) * | 2011-11-15 | 2016-01-06 | 中国科学院光电研究院 | Laser spectrum inducing component detection system |
| CN104535290A (en) * | 2014-12-30 | 2015-04-22 | 黄真理 | Laser-induced fluorescence three-dimensional fluid detection system and method |
| CN109884061A (en) * | 2018-12-19 | 2019-06-14 | 长春理工大学 | Utilize the method for common focus point migration microscopic system measuring medium surface roughness |
| CN109884020A (en) * | 2018-12-19 | 2019-06-14 | 长春理工大学 | Using common focus point migration microscopic system to the non-destructive measuring method of micro/nano level Medium Wave Guide or stepped ramp type structure side wall angle |
| CN109884020B (en) * | 2018-12-19 | 2021-07-09 | 长春理工大学 | Nondestructive measurement method for micro-nano dielectric waveguide or step-type structure side wall angle by using confocal laser scanning microscope system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060705 |