CN2447787Y - Capillary cleaner - Google Patents
Capillary cleaner Download PDFInfo
- Publication number
- CN2447787Y CN2447787Y CN 00218803 CN00218803U CN2447787Y CN 2447787 Y CN2447787 Y CN 2447787Y CN 00218803 CN00218803 CN 00218803 CN 00218803 U CN00218803 U CN 00218803U CN 2447787 Y CN2447787 Y CN 2447787Y
- Authority
- CN
- China
- Prior art keywords
- syringe
- kapillary
- spring
- capillary
- rubber stopper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Cleaning In General (AREA)
Abstract
The utility model discloses a capillary cleaner which comprises a syringe and a spring, wherein, the spring is fixed between two clamping plates. The syringe is fixed at one end of each of the clamping plates. A piston of the syringe is driven through the traction force of the spring to generate pressure to continuously press cleaning liquid in the syringe into a capillary. Therefore, the aim of effective cleaning the capillary is reach.
Description
The utility model relates to a kind of washer, refers to a kind of kapillary washer especially.
Usually the capillary inner diameter of Capillary Electrophoresis use is generally 25 μ m-100 μ m, and that wherein the most frequently used is two kinds of 50 and 75 μ m.For new kapillary and untapped kapillary of a specified duration, need to clean successively with 1mol/L NaOH solution, 0.1mol/L NaOH solution, distilled water.Also must clean in some cases with 0.1mol/L HCl solution, methyl alcohol or scaling agent.Must fill the damping fluid that electrophoresis uses in cleaning postcapillary can electrophoresis.Also must mediation after kapillary is blocked.
So in actual Capillary Electrophoresis operating process, to kapillary dredge, cleaning and filling and replacing running buffer be regular work.And existing kapillary cleans and the filling technology is that one in kapillary is sealed in the closed container, and the other end places cleaning fluid, by pump closed container is vacuumized and realizes that kapillary cleans and filling; Or cleaning fluid placed closed container, by compressor pressurizes cleaning fluid is pressed in the kapillary.Typical devices as shown in Figure 1, this method mechanism complexity, operation be loaded down with trivial details, also easily bubble is sucked in the kapillary, reagent consumption is big, instrument is more expensive, and noise is arranged during work, needs electricity consumption.
In view of existing defective in the existing kapillary cleaning device, the purpose of this utility model is to provide a kind of Capillary cleaner simple to operate, easy to use.
Capillary cleaner of the present utility model includes: syringe, clamping plate, spring, sleeve pipe, sheath, rubber stopper.Spring is fixed between two clamping plate, clamping plate are used to connect spring, the pressure of bearing spring, and give syringe outlet and piston two ends with pressure-loaded, syringe outlet closely is connected with the rubber stopper endoporus, spring is fixed on is used to produce pulling force between two clamping plate, syringe outlet is connected with sleeve pipe, syringe is fixed between two clamping plate by sheath and rubber stopper, and sleeve pipe connects syringe ports and enlarges and the rubber stopper contact area, prevents fluid seepage, syringe is used for promoting cleaning fluid or the Capillary Electrophoresis running buffer is pressed into kapillary automatically continuously, sheath is used for fixing syringe, and crooked to prevent it, rubber stopper is used for sealed hair tubule and syringe needle.In rubber stopper, have the hole for inserting kapillary, the cavity of syringe is equipped with cleaning fluid, kapillary can be inserted in the cleaning fluid, utilize the pulling force pushing syringe piston of spring to move generation pressure, cleaning fluid in the syringe or Capillary Electrophoresis running buffer are pressed in the kapillary automatically continuously, thereby reach the purpose of cleaning, mediation kapillary or filling, exchange buffering liquid.Kapillary is inserted in the cleaning fluid by the rubber consent during use, pushes away or draws the piston of syringe that cleaning fluid is entered in the kapillary.When not using, available steel wire etc. insert in the rubber stopper aperture so that its sealing, and prevents liquid leakage, during use, with its dial-out, inserts kapillary and can clean.
The utility model also can adopt the compressed spring type pushing mechanism: the spring that is about to be fixed between two clamping plate changes the fixedly sheet material of usefulness into, and spring is installed in the syringe promote-side, and the pushing syringe piston can inject kapillary to be cleaned continuously with cleaning fluid.
The rubber stopper that the utility model uses also can be the stopper that rubber or elasticity of plastics material are made.
The utility model not only can be used for cleaning and the mediation kapillary, also can be used for kapillary filling and mediation.
Owing to use spring syringe pushing mechanism, make capillary seal thereby not only reach, make syringe ports and hermetically sealed integrated connection of spring pressure formula capillaceous simultaneously, and fix, so adopt the utility model structure by sheath, because good airproof performance, cleaning performance is good, is difficult for producing bubble, and filling and mediation effect are better, the reappearance of mensuration is improved, but and the few and continuous wash of cleaning fluid consumption, general 2 milliliters of cleaning fluids are continuous wash more than 24 hours automatically, and are simple to operate, easy to use, simple in structure, firmly, need not to use oil pump or water pump, avoid producing noise, energy-conservation, reduce environmental pollution.
Now further describe the utility model in conjunction with the accompanying drawings.
Symbol description
1-syringe 2-clamping plate
3-spring 4-sleeve pipe
5-sheath 6-rubber stopper
7-cleaning fluid 10-kapillary
20-connects oil pump, water pump
Fig. 1 is existing kapillary cleaning device.
Fig. 2 is the utility model Capillary cleaner structural representation.
As shown in Figure 2, spring 3 is fixed between two clamping plate 2, syringe 1 port is connected with sleeve pipe 4, syringe 1 is fixed on an end of clamping plate 2 by sheath 5 and rubber stopper 6, have in the rubber stopper and insert kapillary 10 when aperture is used for doing to clean, kapillary 10 can be inserted in the cleaning fluid 7 of syringe cavity, draws or push away the piston of syringe 1, and the cleaning fluid 7 in the syringe 1 is entered in the kapillary 10 automatically continuously.This washer is to be fixed on the two clamping plates 2 and syringe 1 outlet and sleeve pipe 4 tight connections except that spring 3, and other parts all are dismountable, non-caked.
The utility model also can adopt the compressed spring type pushing mechanism: the spring 3 that will be fixed on 2 on two clamping plate changes sheet material into, and spring 3 changes stage clip into, is installed in syringe 1 promote-side, and pushing syringe 1 piston promptly injects cleaning fluid serially and cleans kapillary 10.
Method of operating
Syringe 1 (port has put sleeve pipe) is inhaled 2-3 the milliliter cleaning fluid 7 or the buffer solution of filtering membrane, mouth is discharged bubble up, kapillary is inserted in the hole on the rubber stoppers 6 by clamping plate 2, make kapillary 10 insertion ends pass rubber stopper 6 and expose 1-2 centimetre, rubber stopper 6 endoporus are inserted in syringe 1 outlet, need to guarantee that kapillary 10 insertion ends are in syringe 1 outlet or chamber, left hand is held syringe 1, the right hand will be connected with the promote-side that the clamping plate 2 of spring are moved syringe 1 piston to, and washer promptly enters duty.Experience shows, must make capillary outlet upwards for the kapillary of sky, to discharge the bubble of kapillary inside.
The another kind of using method of this washer is: syringe 1 (port has put sleeve pipe) is inhaled 2-3 the milliliter cleaning fluid 7 or the buffer solution of filtering membrane, mouth is discharged bubble up, with (the back liquid that prevents to pressurize oozes out from the hole in the long 2cm stainless pin insertion of diameter 300 μ m rubber stopper 6 holes, but for new stopper, the deformation force that the hole produces under pressure makes its self-styled better, can not plug in stainless pin), left hand is held syringe, the right hand will be connected with the promote-side that the clamping plate 2 of spring are moved syringe 1 piston to, extract stainless pin, kapillary 10 is passed through the hole that clamping plate 2 insert on the rubber stoppers 6, make kapillary 10 insertion ends pass rubber stopper 6 and expose 1-2 centimetre, guarantee kapillary 10 insertion ends in syringe 1 outlet or chamber, washer promptly enters duty.
Claims (4)
1, a kind ofly is used to clean Capillary cleaner, it is characterized in that it includes syringe, clamping plate, spring, sleeve pipe, sheath, rubber stopper, spring is fixed between two clamping plate, syringe ports is connected with sleeve pipe and be fixed on an end of clamping plate by sheath and rubber stopper, has in the rubber stopper and can insert hole capillaceous to be cleaned.
2, Capillary cleaner according to claim 1 is characterized in that also spring to be installed in the syringe promote-side.
3, Capillary cleaner according to claim 1 is characterized in that described rubber stopper can be the stopper that rubber or elasticity of plastics material are made.
4, Capillary cleaner according to claim 1 is characterized in that also can be used as Yu Guanchong, mediation kapillary.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 00218803 CN2447787Y (en) | 2000-08-11 | 2000-08-11 | Capillary cleaner |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 00218803 CN2447787Y (en) | 2000-08-11 | 2000-08-11 | Capillary cleaner |
Publications (1)
Publication Number | Publication Date |
---|---|
CN2447787Y true CN2447787Y (en) | 2001-09-12 |
Family
ID=33584110
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 00218803 Expired - Fee Related CN2447787Y (en) | 2000-08-11 | 2000-08-11 | Capillary cleaner |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN2447787Y (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102220107A (en) * | 2011-04-19 | 2011-10-19 | 北京鑫诺美迪基因检测技术有限公司 | Sequencer capillary tube cleaning reagent |
CN102253104A (en) * | 2011-03-25 | 2011-11-23 | 浙江大学 | Automatic cleaning and glue pouring platform for capillary array |
CN104792905A (en) * | 2015-04-13 | 2015-07-22 | 中国科学院化学研究所 | Micron-sized zone sampling method and device suitable for capillary tube |
-
2000
- 2000-08-11 CN CN 00218803 patent/CN2447787Y/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102253104A (en) * | 2011-03-25 | 2011-11-23 | 浙江大学 | Automatic cleaning and glue pouring platform for capillary array |
CN102253104B (en) * | 2011-03-25 | 2013-05-22 | 浙江大学 | Automatic cleaning and glue pouring platform for capillary array |
CN102220107A (en) * | 2011-04-19 | 2011-10-19 | 北京鑫诺美迪基因检测技术有限公司 | Sequencer capillary tube cleaning reagent |
CN104792905A (en) * | 2015-04-13 | 2015-07-22 | 中国科学院化学研究所 | Micron-sized zone sampling method and device suitable for capillary tube |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |