CN221117441U - Device for optimizing living sperm - Google Patents

Abstract

The utility model relates to the field of medical equipment, and discloses a device for optimizing living sperm, which comprises the following components: the device comprises a bracket, an upper imaging unit, a lower imaging unit, an objective table, a sample pool and an image analysis unit, wherein the upper imaging unit and the lower imaging unit are sequentially and fixedly arranged on the bracket, and objective lenses are oppositely arranged; the object stage is arranged on the bracket and is positioned between the upper imaging unit and the lower imaging unit; the objective table comprises a first plate, a second plate and an adapter plate which are vertically stacked, wherein the first plate is arranged on the surface of the second plate in a sliding manner along a first direction; the second plate is slidably arranged on the surface of the adapter plate along a second direction perpendicular to the first direction; the adapter plate is fixedly connected with the bracket; the first plate is provided with a first through hole; the top of the sample pool is open, the bottom of the sample pool is transparent, and the sample pool is erected in the first through hole; the image analysis unit is electrically connected with the upper imaging unit and the lower imaging unit. The system provided by the utility model can be used for rapidly switching the observation position, so that the observation of the sample in the sample cell is more accurate.

Description

Device for optimizing living sperm

Technical Field

The utility model relates to the technical field of microscopic imaging for semen inspection, in particular to a device for optimizing living sperm.

Background

The existing medical clinical detection adopts different microscopic detection methods to detect the concentration, activity and morphology of sperm respectively.

The motility of the sperms can be completed manually or by computer assistance, the movement speed of the sperms is extremely high and is tens of micrometers/second, and continuous observation and accurate observation are required. In addition, when the mirror motion is observed, the interference of external factors needs to be effectively eliminated. Such as stability of the scope, traceability of the instrument. When separating different types of sperms, the precision recognition and the precision moving degree are required to be achieved, and damage to the sperm sample in the moving process is avoided. Furthermore, in the performance research process of sperms, effective supervision and retention of research data are required, so that subsequent retrieval and tracing are facilitated.

In view of the lack of such devices in the prior art, it is highly desirable to provide a preferred solution for living sperm in order to improve the accuracy of observation and separation of living sperm and reduce the interference of the external environment with the observation and separation processes.

Disclosure of Invention

To address at least one or more of the technical problems mentioned above, embodiments of the present utility model provide a device for in vivo sperm preference comprising: the device comprises a bracket, an upper imaging unit, a lower imaging unit, an objective table, a sample pool and an image analysis unit, wherein the upper imaging unit and the lower imaging unit are vertically kept at intervals and are sequentially and fixedly arranged on the bracket; the objective lenses of the upper imaging unit and the lower imaging unit are oppositely arranged; the object stage is arranged on the bracket and is positioned between the upper imaging unit and the lower imaging unit; the objective table comprises a first plate, a second plate and an adapter plate which are vertically stacked, wherein the first plate is slidably arranged on the surface of the second plate along a first direction; the second plate can be arranged on the surface of the adapter plate in a sliding manner along a second direction perpendicular to the first direction; the adapter plate is fixedly connected with the bracket; the first plate is provided with a first through hole; the second plate is provided with a second through hole corresponding to the first through hole; the adapter plate is provided with a third through hole corresponding to the first through hole; the top of the sample pool is open, the bottom of the sample pool is transparent, and the sample pool is erected in the first through hole; the image analysis unit is electrically connected with the imaging unit and the lower imaging unit.

According to one embodiment of the utility model, the first plate and the second plate are connected through a sliding rail and a sliding block, and the second plate is provided with a first motor for driving the first plate to slide relative to the second plate; the second plate is connected with the adapter plate through a sliding rail and a sliding block, and a second motor for driving the second plate to slide relative to the adapter plate is arranged on the second plate.

According to an embodiment of the present utility model, an imaging field of view of the upper imaging unit is larger than an imaging field of view of the lower imaging unit; the positions of the upper imaging unit and the lower imaging unit are set as follows: the imaging center of the lower imaging unit is located within the imaging field of view of the upper imaging unit.

According to one embodiment of the utility model, the first through hole is embedded with a bracket, and the bracket is provided with an accommodating groove with an opening at the bottom; the size of the accommodating groove is matched with that of the sample pool; a heating plate surrounding the accommodating groove is arranged in the bracket; two sides of the accommodating groove are provided with abdication grooves; the side wall of the accommodating groove is provided with an elastic locating pin.

According to one embodiment of the utility model, a temperature control device connected with the heating plate is arranged in the bracket.

According to one embodiment of the present utility model, the preferred device for living sperm further comprises an optical tweezer unit and a micro-manipulation unit, the optical pickup unit being disposed on the support and configured to: the irradiation area covers the bottom of the sample cell through the third through hole and the second through hole; the micro-operation unit is arranged on the bracket and is set as follows: the sample of the cuvette is handled through the top opening of the cuvette.

According to one embodiment of the utility model, the micro-operation unit comprises a four-dimensional operation table and a microinjection device controlled by the four-dimensional operation table, wherein the four-dimensional operation table comprises a fixed seat, an X-axis chassis, a Y-axis sliding block, a Z-axis sliding block, a D-axis fixed block, a D-axis sliding block, a clamp rod and a clamp which are sequentially connected, and the four-dimensional operation table is provided with: the X-axis chassis can slide along the X direction relative to the fixed seat; the Y-axis chassis can slide along the Y direction relative to the X-axis chassis; the Y-axis sliding block can slide relative to the Y-axis chassis; the Z-axis sliding block can vertically slide relative to the Y-axis sliding block; the D-axis fixed block can rotate relative to the Z-axis sliding block; the D-axis sliding block can slide relative to the D-axis fixed block; the clamp rod is fixedly connected with the D-axis sliding block; the clamp is fixedly connected with the clamp rod; the clamp clamps the microinjection device.

According to one embodiment of the utility model, the microinjection device comprises a microinjection instrument, a stepping motor and a gear transmission group, wherein the stepping motor is connected with the microinjection instrument through the gear transmission group.

According to one embodiment of the utility model, the support comprises a pneumatic optical platform provided with pneumatic vibration-proof means for automatically adjusting the flatness.

According to one embodiment of the utility model, the device for in vivo sperm preference further comprises a storage unit, said storage unit being connected to said image analysis unit.

By providing the device for living sperm preference as provided above, the embodiment of the utility model can quickly switch the observation position by arranging the object stage which can move in two mutually perpendicular directions of the horizontal plane, thereby forming the tracking of living sperm and ensuring more accurate observation of the sample in the sample cell. The sliding rail and the sliding block are driven by the motor to mutually slide so as to realize the translation of the object stage, so that the amplitude degree formed in the translation process is small, the stability of a sperm sample in the sample pool is kept, and the accuracy of observation and separation is improved. By arranging imaging units with different imaging fields and observing the sample at the same time, the observation precision of the living sperm sample can be improved. Through setting up hot plate and temperature control device at the bracket, can realize that the living sperm constant temperature in the sample cell is preserved, reduce the interference of the observation that temperature variation leads to. The precise preferential separation of the living sperm is realized through the optical tweezers unit and the micro-operation unit. Through setting up the little operating unit that has four-dimensional moving structure, can realize the absorption of multiple angle to improve the precision of selecting the separation of living sperm. By arranging the pneumatic optical platform with the automatic leveling function, the living sperm observation and the preferential separation stability are realized. By setting the storage unit, the normal operation of the system and the storage of test data are realized, and the information tracing is realized.

Drawings

The above, as well as additional purposes, features, and advantages of exemplary embodiments of the present utility model will become readily apparent from the following detailed description when read in conjunction with the accompanying drawings. In the drawings, embodiments of the utility model are illustrated by way of example and not by way of limitation, and like reference numerals refer to similar or corresponding parts and in which:

FIG. 1 shows a schematic view of an apparatus for in vivo sperm preference in accordance with an embodiment of the utility model;

FIG. 2 shows a schematic diagram of an upper imaging unit and stage according to an embodiment of the utility model;

Fig. 3 shows a schematic diagram of an image analysis unit according to an embodiment of the utility model;

FIG. 4 shows a schematic diagram of a lower imaging unit according to an embodiment of the utility model;

FIG. 5 shows a schematic view of a tray and a cuvette according to an embodiment of the utility model;

FIG. 6 shows a schematic longitudinal cross-sectional view of a carrier and a cuvette according to an embodiment of the utility model;

FIG. 7 shows a schematic diagram of an optical tweezer unit according to an embodiment of the present utility model;

FIG. 8 shows a schematic diagram of a four-dimensional console according to an embodiment of the utility model;

fig. 9 shows a schematic view of a microinjection apparatus according to an embodiment of the present utility model;

FIG. 10 shows a schematic diagram of an aerodynamic optical platform according to an embodiment of the utility model.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be understood that the terms "comprises" and "comprising," when used in this specification and in the claims, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.

It is also to be understood that the terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. As used in the specification and claims, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should be further understood that the term "and/or" as used in the present specification and claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.

As used in this specification and the claims, the term "if" may be interpreted as "when..once" or "in response to a determination" or "in response to detection" depending on the context. Similarly, the phrase "if a determination" or "if a [ described condition or event ] is detected" may be interpreted in the context of meaning "upon determination" or "in response to determination" or "upon detection of a [ described condition or event ]" or "in response to detection of a [ described condition or event ]".

Specific embodiments of the present utility model are described in detail below with reference to the accompanying drawings.

Fig. 1 shows a schematic view of a device for in vivo sperm preference according to an embodiment of the utility model.

Fig. 2 shows a schematic diagram of an upper imaging unit and stage according to an embodiment of the utility model.

As shown in fig. 1, a preferred device for living sperm comprises: the device comprises a bracket 1, an upper imaging unit 11, a lower imaging unit 12, an objective table 13, a sample cell 14 and an image analysis unit 15, wherein the upper imaging unit 11 and the lower imaging unit 12 are vertically kept at intervals and are sequentially and fixedly arranged on the bracket 1; the objective lenses of the upper imaging unit 11 and the lower imaging unit 12 are disposed opposite to each other; a stage 13 is provided on the stand 1 between the upper imaging unit 11 and the lower imaging unit 12.

As shown in fig. 2, the stage 13 includes a first plate 131, a second plate 132, and an adapter plate 133 arranged in a vertical stack, the first plate 131 being slidably disposed on a surface of the second plate 132 in a first direction; the second plate 132 is slidably disposed on a surface of the adapter plate 133 in a second direction perpendicular to the first direction; the adapter plate 133 is fixedly connected with the bracket 1; the first plate 131 is provided with a first through hole; the second plate 132 is provided with a second through hole corresponding to the first through hole; the adapter plate 133 is provided with a third through hole corresponding to the first through hole; the top of the sample cell 14 is open, the bottom is transparent, and the sample cell is erected in the first through hole; the image analysis unit 15 is electrically connected to the imaging unit and the lower imaging unit 12.

The support 1 is used for providing support for the upper imaging unit 11, the lower imaging unit 12, the stage 13, the sample cell 14 and the image analysis unit 15, so that the relative fixed position relation among the supported units is maintained. For example, the up-down positional relationship between the upper imaging unit 11 and the lower imaging unit 12, the positional relationship between the stage 13 and the sample cell 14 located between the upper imaging unit 11 and the lower imaging unit 12, and the like.

The upper imaging unit 11 and the lower imaging unit 12 are microscopes, respectively, with lenses thereof disposed in opposition. That is, the lens of the upper imaging unit 11 is downward, and the lens of the lower imaging unit 12 is upward. Wherein the upper imaging unit 11 may further include an illumination means and an objective lens elevation means for providing illumination and adjusting a focal length for observing living sperm in the sample cell 14. The upper imaging unit 11 and the lower imaging unit 12 may employ existing commercially available optical microscopes or electron microscopes, and the specific type of the present utility model is not limited.

The upper imaging unit 11 and the lower imaging unit 12 are fixedly arranged, which is beneficial to adjusting the focal length, initializing and the like of the precise instruments and reducing the interference caused by the environment in the use process of the precise instruments.

As shown in fig. 2, the table top of the objective table 13 is provided with a hollow structure, the sample cell 14 is embedded in the hollow structure of the objective table 13, and above the sample cell 14, the upper imaging unit 11 observes and images, and below the sample cell 14, the lower imaging unit 12 observes and images. It should be noted that in the embodiment of the present utility model, the sample cell 14 is made of transparent material at least at the bottom. Alternatively, the entire cuvette 14 is made of transparent material.

Stage 13 may carry a cuvette 14 for translation between upper imaging unit 11 and lower imaging unit 12. For example, when the upper imaging unit 11 performs observation with a large field of view on living sperm in the sample cell 14 and the lower imaging unit 12 performs fine observation of the observation range of the upper imaging unit 11, the stage 13 is required to carry the sample cell 14 and perform a translation operation with reference to the objective lens of the lower imaging unit 12, so that the lower imaging unit 12 performs a full scan of the observation range.

The stage 13 is realized in such a manner as to carry the translation of the cuvette 14 by providing a multi-layered structure, i.e., a first plate 131, a second plate 132 and an adapter plate 133 stacked in the vertical direction. The adaptor plate 133 is fixedly disposed, and the second plate 132 slides in a first direction, for example, a left-right direction, on an upper surface of the adaptor plate 133. The first plate 131 slides in a second direction, e.g., a front-rear direction, on the upper surface of the second plate 132. Thereby realizing a fast and stable translation of the cuvette 14, and maintaining the cuvette 14 stable during the realization of a fast scanning of the lower imaging unit 12 over the observation range of the upper imaging unit 11.

Further, by providing the first through hole, the second through hole, and the third through hole in the first plate 131, the second plate 132, and the adapter plate 133, it is achieved that observation can be made from both the upper and lower directions of the sample cell 14.

Fig. 3 shows a schematic diagram of an image analysis unit according to an embodiment of the utility model.

As shown in fig. 3, the image analysis unit 15 may be mounted on the stand 1 as shown in fig. 1, or may be set at a position selected by itself as needed. The image analysis unit 15 is electrically connected with the upper imaging unit 11 and the lower imaging unit 12 to realize transmission of imaging information, the image analysis unit 15 can be realized by carrying corresponding image analysis software on computer hardware, and existing or future-developed or commercial image analysis equipment can be adopted, and the utility model is not limited in specific type and software aspects.

When observing living sperm, firstly placing a sample into the sample cell 14, then placing the sample cell 14 into the objective table 13, then adjusting objective lenses of the upper imaging unit 11 and the lower imaging unit 12, observing the sample in the sample cell 14, and when the sample needs to be translated, driving the sample cell 14 to carry out translation operation through translation of different layers of the objective table 13. The image information acquired by the upper imaging unit 11 and the lower imaging unit 12 is transmitted to the image analysis unit 15 in real time, and the observation result is output by the image analysis unit 15.

In fig. 2, a first plate 131 and a second plate 132 are connected through a sliding rail and a sliding block, and a first motor for driving the first plate 131 to slide relative to the second plate 132 is arranged on the second plate 132; the second plate 132 is connected with the adapter plate 133 through a sliding rail and a sliding block, and a second motor for driving the second plate 132 to slide relative to the adapter plate 133 is arranged on the second plate 132.

The first motor and the second motor can select the stepping motor 23 or the frequency modulation motor to realize the fine control of the sliding action, and the sliding action is performed through the mutual matching of the sliding rail and the sliding block, so that the formed vibration is small, the stability of the sample cell 14 is maintained, and the interference caused by the vibration can be eliminated.

In particular, the first motor and the second motor may be of the same or different types and step sizes. Preferably, the first motor and the second motor are identical in model number.

Fig. 4 shows a schematic diagram of a lower imaging unit according to an embodiment of the utility model.

According to one embodiment of the present utility model, the imaging field of view of the upper imaging unit 11 is larger than the imaging field of view of the lower imaging unit 12; the positions of the upper imaging unit 11 and the lower imaging unit 12 are set as follows: the imaging center of the lower imaging unit 12 is located within the imaging field of view of the upper imaging unit 11.

The larger field of view microscope has a smaller resolution than the smaller field of view microscope. When the sperm sample is observed, microscopes with different rates of separation are used for observation, so that sperm motility distribution in the sample can be characterized as a whole, and regional samples can be tracked locally. By the above-described upper imaging unit 11 and lower imaging unit 12 having different resolutions being formed in cooperation, observation of a sample from a part and a whole can be achieved.

According to one embodiment of the present utility model, it is also possible to employ that the imaging field of view of the lower imaging unit 12 is larger than that of the upper imaging unit 11.

Fig. 5 shows a schematic view of a carrier and a cuvette according to an embodiment of the utility model.

Fig. 6 shows a schematic longitudinal cross-sectional view of a carrier and a cuvette according to an embodiment of the utility model.

According to one embodiment of the present utility model, the bracket 141 is embedded in the first through hole, and as shown in fig. 5 and 6, the bracket 141 is provided with an accommodating groove with an opening at the bottom; the size of the receiving groove is matched with that of the sample cell 14; a heating plate 142 surrounding the receiving groove is provided in the bracket 141; two sides of the accommodating groove are provided with abdication grooves; the side wall of the accommodating groove is provided with an elastic locating pin.

The outer edge of the bracket 141 is erected in the first through hole, and the bottom center of the accommodating groove is provided with an opening, which is communicated with the second through hole and the third through hole as an observation hole of the lower imaging unit 12. The size of the holding groove is matched with that of the sample cell 14, so that when the sample cell 14 is embedded in the holding groove, the holding groove forms a clamping effect on the sample cell 14, and the stability of the sample cell 14 is maintained.

Around the holding groove, a heating plate 142 is provided, the heating plate 142 is embedded in the side wall of the holding groove, the heating plate 142 is supported by a bottom plate 143, and the bottom plate 143 is fixedly connected with the bracket 141. The sample cell 14 may be heated by the heating plate 142 so as to maintain the sample in the sample cell 14 in a constant temperature state.

The two sides of the holding groove are also provided with a yielding groove which is used for conveniently placing or taking the sample cell 14.

In order to more stably fix the sample cell 14, a plurality of symmetrically arranged elastic positioning pins are also arranged on the side wall of the accommodating groove. When the sample cell 14 is embedded in the accommodating groove, the elastic positioning pin is ejected out of the side wall of the positioning groove to clamp the side wall of the sample cell 14.

According to one embodiment of the present utility model, a temperature control device is provided within bracket 141 that is coupled to heating plate 142.

The temperature control device comprises a temperature sensor and a temperature control chip, wherein the temperature sensor is used for sensing the temperature of the sample cell 14 and transmitting a temperature signal to the temperature control chip, and the temperature control chip controls whether the heating plate 142 is electrified or not according to a preloaded program, so that accurate control of the temperature is realized, and a constant-temperature environment is provided for a sample in the sample cell 14.

According to one embodiment of the utility model, the device for living sperm preference further comprises an optical tweezer unit 17 and a micro-manipulation unit, the optical pick-up unit being arranged on the support 1, arranged to: the irradiation region covers the bottom of the sample cell 14 through the third through-hole and the second through-hole; the micro-operation unit is arranged on the bracket 1 and is set as follows: the sample of the cuvette 14 is handled through the top opening of the cuvette 14.

When the preferred separation operation is required for the living sperm, the sperm to be preferably separated is positioned by the optical tweezer unit 17, and sucked by the micro-operation unit, thereby being separated from the sample cell 14.

Fig. 7 shows a schematic diagram of an optical tweezer unit according to an embodiment of the utility model.

As shown in fig. 7, the irradiation area of the optical tweezer unit 17 refers to a range that the optical tweezer unit 17 can cover by adjusting the illumination angle, and in the embodiment of the present utility model, the distance and angle between the optical tweezer unit 17 and the sample cell 14 can be adjusted. The irradiation region covers the bottom of the sample cell 14 through the third through hole and the second through hole, meaning that the distance and angular relationship between the optical tweezer unit 17 and the lower imaging unit 12, and between the optical tweezer unit and the sample cell 14 satisfy the above requirements.

In an embodiment of the present utility model, the micro-manipulation unit needs to be operated from the upper opening of the sample cell 14. Therefore, the optical tweezers unit 17 is arranged from the lower direction of the sample pool 14 to irradiate, mutual interference between the optical tweezers unit 17 and the micro-operation unit is avoided, and as the irradiation light of the optical tweezers unit 17 is from bottom to top, when the micro-operation unit is operated to separate, the visual angle is from top to bottom, the direction of the optical fiber is reversely irradiated, so that the optical tweezers unit can accurately identify, and the accuracy of separation operation can be improved.

In addition, the relative positions of the optical tweezer unit 17 and the lower imaging unit 12 are fixed, and the irradiation area of the optical tweezer unit 17 can be adjusted by adjusting the irradiation angle of the optical tweezer unit 17, so that the optical tweezer unit 17 and the lower imaging unit 12 can be arranged relatively close, and mutual interference is avoided.

The optical tweezer unit 17 can be realized by using an existing or commercially available optical tweezer device, and the present utility model is not limited thereto.

FIG. 8 shows a schematic diagram of a four-dimensional console according to an embodiment of the utility model.

According to one embodiment of the utility model, the micro-manipulator unit comprises a four-dimensional manipulator stage 16 and a microinjection device 2 controlled by the four-dimensional manipulator stage 16.

As shown in fig. 8, the four-dimensional console 16 includes a fixed base 161, an X-axis chassis 162, a Y-axis chassis 163, a Y-axis slider 164, a Z-axis slider 165, a D-axis fixed block 166, a D-axis slider 167, a clamp lever 168, a clamp 169, which are sequentially connected, and is provided: the X-axis chassis 162 is slidable along the X-direction relative to the fixing base 161; the Y-axis chassis 163 is slidable in the Y direction relative to the X-axis chassis 162; the Y-axis sliding block 164 can slide relative to the Y-axis chassis; the Z-axis sliding block 165 is vertically slidable with respect to the Y-axis sliding block 164; the D-axis fixed block 166 is rotatable relative to the Z-axis sliding block 165; the D-axis sliding block 167 is slidable with respect to the D-axis fixing block 166; the clamp lever 168 is fixedly connected with the D-axis sliding block 167; the clamp 169 is fixedly connected with the clamp rod 168; the clamp 169 holds the microinjection apparatus 2.

The micro-manipulation unit is used for performing a preferred separation operation on the sample, providing an angle of operation through the four-dimensional manipulation stage 16, and providing a suction separation operation through the microinjection apparatus 2.

The four-dimensional manipulation stage 16 has a plurality of independent degrees of freedom of manipulation.

The base of the four-dimensional operation table 16 is fixedly connected with the bracket 1. The different degrees of freedom are indicated in the present utility model by letters X, Y, Z, D. The X direction and the Y direction can be mutually perpendicular or keep a certain included angle.

The X-axis chassis 162 is slidable along the X-direction relative to the fixing base 161; the Y-axis chassis 163 is slidable in the Y-direction relative to the X-axis chassis 162, providing freedom in two directions on the same plane.

The Y-axis sliding block 164 can slide relative to the Y-axis chassis; the Z-axis sliding block 165 is vertically slidable with respect to the Y-axis sliding block 164; providing freedom in two directions in another plane.

The D-axis fixed block 166 is rotatable relative to the Z-axis sliding block 165; the D-axis sliding block 167 is slidable with respect to the D-axis fixing block 166; a rotational degree of freedom and a sliding degree of freedom in one plane are provided.

Thus, the microinjection device 2 is held by the jig so as to approach the sample cell 14 from a plurality of directions and angles, and a precise sample-preferable separation operation can be performed.

Fig. 9 shows a schematic view of a microinjection apparatus according to an embodiment of the present utility model.

As shown in fig. 9, the microinjection apparatus 2 includes a microinjection instrument 21, a stepping motor 23, and a gear train set 22, and the stepping motor 23 is connected to the microinjection instrument 21 through the gear train set 22.

The power source provided by the stepping motor 23 is used for providing power for the gear set, the gear set drives the oil pressure type microinjection instrument 21 to serve as a load, so that the oil pressure type microinjection instrument 21 works fully automatically, and the structure can realize accurate control of the suction quantity due to higher gear transmission precision.

FIG. 10 shows a schematic diagram of an aerodynamic optical platform according to an embodiment of the utility model.

As shown in fig. 10, the stand 1 includes a pneumatic optical stage 19, and the pneumatic optical stage 19 is provided with a pneumatic vibration damper for automatically adjusting flatness. The pneumatic optical platform 19 is provided with a platform 191 and a supporting leg 192, a buffer damping device is arranged at the connection position of the platform 191 and the supporting leg 192, a length adjusting device is arranged at the bottom of the supporting leg, and a level detector is also arranged at the bottom of the supporting leg, so that a shockproof device is formed together, and the flatness of the horizontal plane of the platform can be automatically adjusted.

The upper imaging unit 11, the lower imaging unit 12, the objective table 13, the sample cell 14, the image analysis unit 15, the optical tweezer unit 17 and the micro-operation unit are all arranged on the pneumatic optical platform 19, and stable support is provided by the pneumatic optical platform 19, so that interference on observation and separation of sperm samples caused by the fact that stable horizontal state cannot be maintained can be effectively eliminated.

According to one embodiment of the utility model, the device for in vivo sperm preference further comprises a storage unit 18, the storage unit 18 being connected to the image analysis unit 15. The storage unit 18 can be realized by a computer, can store programs executed by the image processing unit, data storage and the like, and ensures that all systems normally operate and store test data and trace information. The memory unit 18 may also be an AI algorithm processor for processing and storing intermediate data of the image processing unit.

By providing the device for living sperm preference as provided above, the embodiment of the utility model can quickly switch the observation position by arranging the object stage which can move in two mutually perpendicular directions of the horizontal plane, thereby forming the tracking of living sperm and ensuring more accurate observation of the sample in the sample cell. The sliding rail and the sliding block are driven by the motor to mutually slide so as to realize the translation of the object stage, so that the amplitude degree formed in the translation process is small, the stability of a sperm sample in the sample pool is kept, and the accuracy of observation and separation is improved. By arranging imaging units with different imaging fields and observing the sample at the same time, the observation precision of the living sperm sample can be improved. Through setting up hot plate and temperature control device at the bracket, can realize that the living sperm constant temperature in the sample cell is preserved, reduce the interference of the observation that temperature variation leads to. The precise preferential separation of the living sperm is realized through the optical tweezers unit and the micro-operation unit. Through setting up the little operating unit that has four-dimensional moving structure, can realize the absorption of multiple angle to improve the precision of selecting the separation of living sperm. By arranging the pneumatic optical platform with the automatic leveling function, the living sperm observation and the preferential separation stability are realized. By setting the storage unit, the normal operation of the system and the storage of test data are realized, and the information tracing is realized.

While various embodiments of the present utility model have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous modifications, changes, and substitutions will occur to those skilled in the art without departing from the spirit and scope of the utility model. It should be understood that various alternatives to the embodiments of the utility model described herein may be employed in practicing the utility model. The appended claims are intended to define the scope of the utility model and are therefore to cover all equivalents or alternatives falling within the scope of these claims.

Claims (10)

1. A device for in vivo sperm preference comprising:

A bracket, an upper imaging unit, a lower imaging unit, an objective table, a sample cell and an image analysis unit,

The upper imaging unit and the lower imaging unit are vertically kept at intervals and sequentially and fixedly arranged on the bracket;

the objective lenses of the upper imaging unit and the lower imaging unit are oppositely arranged;

the object stage is arranged on the bracket and is positioned between the upper imaging unit and the lower imaging unit;

The object stage comprises a first plate, a second plate and an adapter plate which are vertically stacked,

The first plate is arranged on the surface of the second plate in a sliding way along the first direction;

The second plate can be arranged on the surface of the adapter plate in a sliding manner along a second direction perpendicular to the first direction;

the adapter plate is fixedly connected with the bracket;

The first plate is provided with a first through hole;

The second plate is provided with a second through hole corresponding to the first through hole;

The adapter plate is provided with a third through hole corresponding to the first through hole;

the top of the sample pool is open, the bottom of the sample pool is transparent, and the sample pool is erected in the first through hole;

the image analysis unit is electrically connected with the upper imaging unit and the lower imaging unit.

2. A device for in vivo sperm preference as described in claim 1, wherein,

The first plate and the second plate are connected through a sliding rail and a sliding block, and a first motor for driving the first plate to slide relative to the second plate is arranged on the second plate;

The second plate is connected with the adapter plate through a sliding rail and a sliding block, and a second motor for driving the second plate to slide relative to the adapter plate is arranged on the second plate.

3. A device for in vivo sperm preference as described in claim 1, wherein,

The imaging field of view of the upper imaging unit is greater than the imaging field of view of the lower imaging unit;

The positions of the upper imaging unit and the lower imaging unit are set as follows: the imaging center of the lower imaging unit is located within the imaging field of view of the upper imaging unit.

4. A device for in vivo sperm preference as described in claim 1, wherein,

A bracket is embedded in the first through hole,

The bracket is provided with an accommodating groove with an opening at the bottom;

the size of the accommodating groove is matched with that of the sample pool;

a heating plate surrounding the accommodating groove is arranged in the bracket;

Two sides of the accommodating groove are provided with abdication grooves;

the side wall of the accommodating groove is provided with an elastic locating pin.

5. A device for in vivo sperm cell preference as claimed in claim 4 wherein,

A temperature control device connected with the heating plate is arranged in the bracket.

6. A device for in vivo sperm preference as described in claim 1, wherein,

Also comprises an optical tweezers unit and a micro-operation unit,

The light shooting unit is arranged on the bracket and is set as follows: the irradiation area covers the bottom of the sample cell through the third through hole and the second through hole;

The micro-operation unit is arranged on the bracket and is set as follows: the sample of the cuvette is handled through the top opening of the cuvette.

7. A device for in vivo sperm preference as described in claim 6, wherein,

The micro-operation unit comprises a four-dimensional operation table and a microinjection device controlled by the four-dimensional operation table,

Four-dimensional operation panel is including fixing base, X axle chassis, Y axle sliding block, Z axle sliding block, D axle fixed block, D axle sliding block, anchor clamps pole, the anchor clamps that connect gradually, sets up to:

The X-axis chassis can slide along the X direction relative to the fixed seat;

The Y-axis chassis can slide along the Y direction relative to the X-axis chassis;

the Y-axis sliding block can slide relative to the Y-axis chassis;

the Z-axis sliding block can vertically slide relative to the Y-axis sliding block;

the D-axis fixed block can rotate relative to the Z-axis sliding block;

The D-axis sliding block can slide relative to the D-axis fixed block;

the clamp rod is fixedly connected with the D-axis sliding block;

the clamp is fixedly connected with the clamp rod;

the clamp clamps the microinjection device.

8. A device for in vivo sperm preference as described in claim 7, wherein,

The microinjection device comprises a microinjection instrument, a stepping motor and a gear transmission group,

The stepping motor is connected with the microinjection instrument through the gear transmission group.

9. A device for in vivo sperm preference as described in claim 1, wherein,

The support comprises a pneumatic optical platform,

The pneumatic optical platform is provided with a pneumatic shockproof device for automatically adjusting the flatness.

10. A device for in vivo sperm preference as described in claim 1, wherein,

Also included is a memory unit that is configured to store,

The storage unit is connected with the image analysis unit.