CN220546835U - Cell culture fluid mixing device - Google Patents
Cell culture fluid mixing device Download PDFInfo
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- CN220546835U CN220546835U CN202321084936.8U CN202321084936U CN220546835U CN 220546835 U CN220546835 U CN 220546835U CN 202321084936 U CN202321084936 U CN 202321084936U CN 220546835 U CN220546835 U CN 220546835U
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- stirring
- cell culture
- wall
- bin
- fixedly arranged
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- 239000012930 cell culture fluid Substances 0.000 title claims description 32
- 238000003756 stirring Methods 0.000 claims abstract description 114
- 238000004113 cell culture Methods 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 238000007790 scraping Methods 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 4
- 238000007599 discharging Methods 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 230000010261 cell growth Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 238000005273 aeration Methods 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 102000014171 Milk Proteins Human genes 0.000 description 1
- 108010011756 Milk Proteins Proteins 0.000 description 1
- 102100037209 Peroxisomal N(1)-acetyl-spermine/spermidine oxidase Human genes 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 235000021239 milk protein Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 108010089000 polyamine oxidase Proteins 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture solution mixing device, which comprises a base, wherein an equipment bin is fixedly arranged at the top of the base, a stirring assembly is fixedly arranged at the top of the equipment bin, the stirring assembly comprises a stirring bin, the stirring bin is fixedly arranged at the top of the equipment bin, a flow-around assembly is fixedly arranged at the top of the base and used for carrying out flow-around on the inside of the stirring bin to promote mixing of cell culture solutions, the technical field of cell culture solution mixing devices is related to, and the flow-around assembly is arranged to convey air flow to a guide pipe and a coil pipe through an air pump, so that the air flow can be conveyed to the inside of the stirring bin while stirring the cell culture solution in the stirring bin, the air flow in the stirring bin can be used for aerating the cell culture solution in the stirring bin, and the exchange speed between the liquids is increased, so that the mixing speed of the cell culture solution is conveniently improved.
Description
Technical Field
The utility model relates to the technical field of cell culture fluid mixing devices, in particular to a cell culture fluid mixing device.
Background
The cell culture fluid refers to a liquid environment for cell growth, generally refers to a complete culture fluid, serum, hydrolyzed milk protein, various additives and the like can be directly used, the serum is required for most cell lines growing and proliferating in a traditional culture medium formula, because most of the independent culture media can not provide all nutrition required by the cell growth, such as MEM culture media, the more common quantity is 5-10%, the serum quantity of a special low-serum culture medium can be reduced to about 3%, the morphology and the function of cells are not affected, the serum can be added after the culture medium is sterilized, the serum can be mixed with the culture medium and filtered, on one hand, some factors required by the cell growth and proliferation, such as growth factors and the like, and on the other hand, the viscosity of the culture medium is increased, so that the damage of cells in suspension culture or after the cells are digested by trypsin can be reduced, however, the addition of serum causes various problems, the serum is produced in batch, the difference between batches is large, the serum contains substances which are toxic to cells, such as polyamine oxidase, complement, antibodies, bacterial toxins and the like, which can affect cell growth and even cause cell death, in order to improve the nutrition supply in cell culture in a cell culture medium, various cell culture liquid components are required to be fully mixed when the cell culture liquid is prepared, a cell culture liquid mixing device is required to be used for uniformly mixing the cell culture liquid, a servo motor is generally used for driving a stirring blade to mix the components in the cell culture liquid when the existing cell culture liquid is mixed, because the liquid lacks aeration and bypass structures of the liquid in the stirring process, so that the liquid is stirred through the simple stirring blade, the working efficiency is lower, and the rapid fusion between liquid and solid structures with different concentrations is not facilitated.
Therefore, based on the above technical problems, it is necessary for those skilled in the art to develop a cell culture liquid mixing device.
Disclosure of Invention
The utility model aims to provide a cell culture fluid mixing device.
In order to achieve the above object, the present utility model provides the following technical solutions:
the cell culture fluid mixing device of the utility model comprises: the device comprises a base, base top fixed mounting equipment bin, equipment bin top fixed mounting stirring subassembly, stirring subassembly includes the stirring bin, stirring bin fixed mounting is at equipment bin top, base top fixed mounting detours the subassembly, detours the subassembly and is used for carrying out detours to the stirring bin inside and promoting cell culture liquid to mix.
Furthermore, universal wheels are rotationally connected to four corners of the bottom of the base, and the universal wheels are used for conveniently driving the base to move.
Further, the stirring assembly comprises a servo motor, the servo motor is fixedly arranged on the inner wall of the equipment bin, the output shaft of the servo motor is fixedly arranged on a driving shaft, and the outer wall of the driving shaft is rotationally connected with the inner wall of the bottom of the stirring bin through a bearing.
Further, stirring sheets are fixedly arranged on the outer wall of the driving shaft, scraping plates are fixedly arranged on the outer wall of the periphery of each stirring sheet, and the scraping plates are in fit contact with the inner wall of the bottom of the stirring bin.
Further, the stirring bin bottom is fixedly provided with a discharging pipe, the discharging pipe penetrates through the equipment bin to extend to the outside of the equipment bin, and the outer wall of the discharging pipe is fixedly provided with a valve.
Furthermore, the top of the stirring bin is matched, inserted and installed with a top cover, and the top cover is detachably connected with the inner wall of the top of the stirring bin through first matching of bolts.
Further, the hand wheel is fixedly installed on the outer wall of the top of the first bolt, the filling pipe is fixedly installed on the outer wall of the top cover and used for filling cell culture fluid raw materials into the stirring bin, the top of the filling pipe is detachably provided with the sealing cover, the inner wall of the top cover is provided with the exhaust hole, and the bottom of the exhaust hole is fixedly provided with the filter screen through the second bolt.
Further, the flow-around assembly comprises an air pump, the air pump is fixedly arranged at the top of the base, a filter element is fixedly arranged at the input end of the air pump, a guide pipe is fixedly arranged at the output end of the air pump, and the guide pipe extends to the inner wall of the bottom of the stirring bin.
Further, pipe tip fixed mounting coil pipe, the coil pipe is located around the stirring storehouse bottom inner wall, the micropore has evenly been seted up to the coil pipe outer wall, pipe outer wall fixed mounting shutoff valve.
In the technical scheme, the cell culture solution mixing device provided by the utility model has the following beneficial effects:
1. through setting up the detour subassembly, carry the air current to leading and the coil pipe through the air pump to make when stirring the inside cell culture liquid of stirring storehouse, can carry the air current to the inside stirring storehouse, make the inside air current of stirring storehouse can carry out the aeration to the inside cell culture liquid of stirring storehouse, increase the exchange rate between the liquid, thereby conveniently improve the mixed speed of cell culture liquid;
2. through setting up servo motor drive stirring leaf, scraper blade, stirring piece and rotate in step to conveniently scrape stirring storehouse bottom through the scraper blade, thereby conveniently avoid cell culture fluid to adsorb the bottom at stirring storehouse and have the stirring dead angle, conveniently when the ejection of compact to cell culture fluid simultaneously, can scrape the cell culture fluid into discharging pipe department and discharge, improved discharging pipe discharge cell culture fluid's efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present utility model, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.
FIG. 1 is a schematic diagram of a front view of a cell culture fluid mixing device according to an embodiment of the present utility model;
FIG. 2 is a schematic diagram of a right side view of a cell culture fluid mixing device according to an embodiment of the present utility model;
FIG. 3 is a schematic diagram showing an exploded top cover structure of a cell culture fluid mixing device according to an embodiment of the present utility model;
FIG. 4 is a schematic diagram of the internal structure of a mixing chamber of a cell culture fluid mixing device according to an embodiment of the present utility model;
FIG. 5 is a schematic view of a servo motor mounting structure of a cell culture fluid mixing device according to an embodiment of the present utility model.
Reference numerals illustrate: base 100, universal wheel 110, equipment bin 120, servo motor 200, drive shaft 210, stirring vane 220, scraper 221, stirring vane 222, stirring bin 230, discharging pipe 231, valve 232, top cover 240, bolt one 250, hand wheel 251, filling pipe 260, sealing cover 261, exhaust hole 270, filter screen 271, bolt two 272, air pump 300, conduit 310, shutoff valve 320, coil 330.
Detailed Description
In order to make the technical scheme of the present utility model better understood by those skilled in the art, the present utility model will be further described in detail with reference to the accompanying drawings.
See fig. 1-5;
the cell culture fluid mixing device of the utility model comprises: the base 100, through setting up the effect that base 100 was used for installing equipment storehouse 120 and stirring subassembly, base 100 top fixed mounting equipment storehouse 120, equipment storehouse 120 top fixed mounting stirring subassembly (not marked in the figure), through setting up the effect that equipment storehouse 120 is used for supporting and installing stirring storehouse 230 and servo motor 200, stirring subassembly includes stirring storehouse 230, stirring storehouse 230 fixed mounting is at equipment storehouse 120 top, base 100 top fixed mounting detours the subassembly, the detours the subassembly and is used for carrying out detours to stirring storehouse 230 inside and promote cell culture liquid to mix, be used for mixing the stirring to cell culture liquid through setting up stirring storehouse 230, be used for leading-in air current to stirring storehouse 230 inside through setting up the detours the subassembly, thereby the molecular movement speed between the cell culture liquid raw materials is promoted through the increase of air current, thereby improve the work efficiency of mixing, base 100 bottom four corners department all rotates and connects universal wheel 110, universal wheel 110 is used for being convenient for driving base 100 and removes, be convenient for driving base 100 through setting up universal wheel 110 and remove.
The stirring assembly comprises a servo motor 200, the servo motor 200 is fixedly arranged on the inner wall of the equipment bin 120, a driving shaft 210 is fixedly arranged on an output shaft of the servo motor 200, the outer wall of the driving shaft 210 is rotationally connected with the inner wall of the bottom of the stirring bin 230 through a bearing, the stirring blade 220 is used for driving the stirring blade 220, the scraping blade 221 and the stirring blade 222 to rotate through the arrangement of the servo motor 200, so that the cell culture liquid is conveniently stirred and mixed through the stirring blade 220, the scraping blade 221 and the stirring blade 222, the mixing speed of each component in the cell culture liquid is improved, the stirring blade 222 is fixedly arranged on the outer wall of the driving shaft 210, the scraping blade 221 is fixedly arranged on the outer wall of the stirring blade 222, the scraping blade 221 is used for mixing each component in the cell culture liquid, the scraping of which is difficult to stir at the bottom of the stirring bin 230 is conveniently arranged through the scraping blade 221, the mixing efficiency of the cell culture liquid is improved, stirring dead angles are avoided, the scraping blade 221 is in matched with the bottom of the stirring bin 230, the bottom of the stirring bin 230 is fixedly arranged on the discharging pipe 231, the discharging pipe 231 penetrates through the equipment bin 120 to extend to the outside the equipment bin 120, the effect of conveniently guiding the cell culture liquid inside the stirring bin 230 is conveniently carried out through the setting of the discharging pipe 231, and the discharging pipe is fixedly arranged on the outer wall of the stirring pipe 231, and the valve 232 is used for controlling the discharging pipe 231.
Top cover 240 is installed in cooperation grafting at stirring storehouse 230 top, can dismantle the connection through the cooperation of bolt one 250 between top cover 240 and the stirring storehouse 230 top inner wall, and bolt one 250 top outer wall fixed mounting hand wheel 251, top cover 240 top outer wall fixed mounting filling pipe 260, filling pipe 260 are used for filling cell culture liquid raw materials to stirring storehouse 230 inside, and the sealed lid 261 of filling pipe 260 top demountable installation has seted up exhaust hole 270, and filter screen 271 is installed through bolt two 272 fixed to exhaust hole 270 bottom.
Through setting up top cap 240 and being used for sealing the top of stirring storehouse 230 to avoid stirring storehouse 230 during operation impurity to fall into in stirring storehouse 230 and cause the pollution to the cell culture liquid, improved the guard action to the cell culture liquid, be used for carrying out fixed connection with between top cap 240 and the stirring storehouse 230 top through setting up bolt one 250, avoid leading into after the air current to stirring storehouse 230 inside, lead to, stirring storehouse 230 inside air current too much leads to top cap 240 to be turned over, be used for improving the stability when top cap 240 installs through setting up bolt one 250, be used for being convenient for with stirring storehouse 230 in unnecessary air current outside of leading out to stirring storehouse 230 through setting up exhaust hole 270, be used for when leading out the air current in the discharge stirring storehouse 230 through setting up filter screen 271, can break the bubble, thereby avoid the air current to take away the cell culture liquid when discharging.
The bypass subassembly includes air pump 300, be used for carrying the effect of air current to stirring storehouse 230 inside through setting up air pump 300, through connect the nitrogen bottle at stirring storehouse 230's input (not annotate in the picture), nitrogen gas in with the nitrogen bottle is pumped to stirring storehouse 230 through air pump 300, through set up the air current into the nitrogen gas have avoid with cell culture liquid between take place the reaction, improve the protection to cell culture liquid, air pump 300 fixed mounting is at the base 100 top, air pump 300 input fixed mounting has the filter core, air pump 300 output fixed mounting pipe 310, through setting up pipe 310 and be arranged in leading-in the coil pipe 330 with the air current, pipe 310 extends to stirring storehouse 230 bottom inner wall, coil pipe 330 sets up to the ring, thereby the air current is evenly carried around stirring storehouse 230 bottom inner wall through coil pipe 330, thereby carry out the omnidirectional bypass to stirring storehouse 230 inside cell culture liquid through the air current in the at utmost, the dead angle of stirring mixing is carried out to the cell culture liquid to the reduction, improve stirring mixing's efficiency, pipe 310 tip fixed mounting coil pipe 330 is located stirring storehouse 230 bottom inner wall, the closure valve 320 is evenly offered to the outer wall (the picture is evenly offered to the pipe 320), the pipe 320 is used for setting up the closure valve 320 and is closed by the fixed stop valve 320.
The utility model has the following specific use process: when the air pump is used, a person skilled in the art fixedly installs the filter screen 271 on the outer wall of the bottom of the air exhaust hole 270 through the second bolt 272, the top cover 240 is inserted and installed on the top of the stirring bin 230, the top cover 240 and the stirring bin 230 are detachably and fixedly connected through the first bolt 250, then all components of the cell culture fluid to be mixed are led into the stirring bin 230 through the filling pipe 260, then the sealing cover 261 is installed inside the filling pipe 260, then the sealing cover 261 is installed on the top of the filling pipe 260, then the input end of the air pump 300 is fixedly connected with a nitrogen cylinder, the servo motor 200 is started, the servo motor 200 drives the driving shaft 210, the stirring blade 220, the scraping blade 221 and the stirring blade 222 to rotate, all components of the cell culture fluid inside the stirring bin 230 are mixed through the driving shaft 210, the stirring blade 220 and the stirring blade 221, and the stirring blade 222, meanwhile the driving air pump 300 is used for pumping nitrogen into the guide pipe 310 and the micro holes on the surface of the coil 330 to slowly charge the cell culture fluid to bypass, and the rapid mixing of all the components of the cell culture fluid is promoted.
While certain exemplary embodiments of the present utility model have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the utility model, which is defined by the appended claims.
Claims (9)
1. A cell culture fluid mixing device comprising: base (100), base (100) top fixed mounting equipment storehouse (120), equipment storehouse (120) top fixed mounting stirring subassembly, stirring subassembly includes stirring storehouse (230), stirring storehouse (230) fixed mounting is at equipment storehouse (120) top, a serial communication port, base (100) top fixed mounting detours the subassembly, detours the subassembly and is used for carrying out detours the promotion cell culture liquid to stirring storehouse (230) inside and mixes.
2. The cell culture fluid mixing device according to claim 1, wherein the universal wheels (110) are rotatably connected to four corners of the bottom of the base (100), and the universal wheels (110) are used for facilitating movement of the base (100).
3. The cell culture fluid mixing device according to claim 1, wherein the stirring assembly comprises a servo motor (200), the servo motor (200) is fixedly installed on the inner wall of the equipment bin (120), an output shaft of the servo motor (200) is fixedly installed on a driving shaft (210), and the outer wall of the driving shaft (210) is rotatably connected with the inner wall of the bottom of the stirring bin (230) through a bearing.
4. A cell culture fluid mixing device according to claim 3, wherein a stirring blade (222) is fixedly arranged on the outer wall of the driving shaft (210), a scraping plate (221) is fixedly arranged on the outer wall around the stirring blade (222), and the scraping plate (221) is in fit contact with the inner wall at the bottom of the stirring bin (230).
5. The cell culture fluid mixing device according to claim 4, wherein a discharge pipe (231) is fixedly arranged at the bottom of the stirring bin (230), the discharge pipe (231) penetrates through the equipment bin (120) to extend to the outside of the equipment bin (120), and a valve (232) is fixedly arranged on the outer wall of the discharge pipe (231).
6. The cell culture fluid mixing device according to claim 1, wherein a top cover (240) is mounted on the top of the stirring bin (230) in a matched and spliced manner, and the top cover (240) is detachably connected with the inner wall of the top of the stirring bin (230) in a matched manner through a first bolt (250).
7. The cell culture fluid mixing device according to claim 6, wherein a hand wheel (251) is fixedly arranged on the outer wall of the top of the first bolt (250), a filling pipe (260) is fixedly arranged on the outer wall of the top cover (240), the filling pipe (260) is used for filling cell culture fluid raw materials into the stirring bin (230), a sealing cover (261) is detachably arranged on the top of the filling pipe (260), an exhaust hole (270) is formed in the inner wall of the top cover (240), and a filter screen (271) is fixedly arranged at the bottom of the exhaust hole (270) through a second bolt (272).
8. The cell culture fluid mixing device according to claim 1, wherein the flow around assembly comprises an air pump (300), the air pump (300) is fixedly arranged at the top of the base (100), a filter element is fixedly arranged at the input end of the air pump (300), a guide pipe (310) is fixedly arranged at the output end of the air pump (300), and the guide pipe (310) extends to the inner wall at the bottom of the stirring bin (230).
9. The cell culture fluid mixing device according to claim 8, wherein a coil (330) is fixedly installed at the end of the conduit (310), the coil (330) is located around the inner wall of the bottom of the stirring bin (230), micropores are uniformly formed in the outer wall of the coil (330), and a shutoff valve (320) is fixedly installed on the outer wall of the conduit (310).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321084936.8U CN220546835U (en) | 2023-05-08 | 2023-05-08 | Cell culture fluid mixing device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202321084936.8U CN220546835U (en) | 2023-05-08 | 2023-05-08 | Cell culture fluid mixing device |
Publications (1)
Publication Number | Publication Date |
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CN220546835U true CN220546835U (en) | 2024-03-01 |
Family
ID=90002791
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN202321084936.8U Active CN220546835U (en) | 2023-05-08 | 2023-05-08 | Cell culture fluid mixing device |
Country Status (1)
Country | Link |
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CN (1) | CN220546835U (en) |
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2023
- 2023-05-08 CN CN202321084936.8U patent/CN220546835U/en active Active
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