CN219297467U - Automatic equipment for picking single bacteria - Google Patents

Automatic equipment for picking single bacteria Download PDF

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Publication number
CN219297467U
CN219297467U CN202223264833.8U CN202223264833U CN219297467U CN 219297467 U CN219297467 U CN 219297467U CN 202223264833 U CN202223264833 U CN 202223264833U CN 219297467 U CN219297467 U CN 219297467U
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picking
culture dish
subassembly
test
pick
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CN202223264833.8U
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梅岩
谢燕娟
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Nanjing Lingxin Biotechnology Co ltd
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Nanjing Lingxin Biotechnology Co ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The utility model discloses an automatic device for picking single bacteria, which is characterized by comprising a machine body, wherein a picking cavity is arranged in the machine body, and the automatic device is characterized in that: the utility model provides a test tube rack, including the cavity of picking, the cavity is provided with tray mechanism, pick mechanism and test-tube rack in, tray mechanism includes tray subassembly and adjusting part, adjusting part connects tray subassembly and organism to be used for adjusting the angle of tray subassembly, the tray subassembly is used for placing the culture dish of cultivateing the bacterial colony, pick the mechanism including picking subassembly and recognition element, recognition element is used for recognizing the position of bacterial colony in the culture dish, pick the subassembly and be used for picking the bacterial colony in the culture dish, the test-tube rack is arranged in one side of picking the mechanism, the test-tube rack is used for placing and collects the test tube, it is used for collecting the bacterial colony after picking the bacterial colony to pick the mechanism to collect the test tube.

Description

Automatic equipment for picking single bacteria
Technical Field
The utility model relates to the technical field of cell picking, in particular to an automatic device for single-bacterium picking.
Background
With the continuous change of the environment, the variety of bacteria exposed to the environment is increased, so that when medical research is carried out, bacteria are often required to be analyzed after being cultivated, when bacteria are cultivated, a culture dish is required to be used for cultivation, and when single bacterial colonies are formed, analysis and detection are carried out by picking the single bacterial colonies.
The existing bulletin number is: chinese patent CN212316118U discloses a colony picking instrument, comprising a base plate, a turntable rotatably mounted on the base plate, a plurality of groups of needle picking devices arranged on the turntable at intervals along the circumferential direction of the turntable, and a plurality of groups of colony picking devices sequentially distributed along the rotation direction of the turntable; the bacterial colony picking device comprises a culture dish moving mechanism, an inoculation pore plate moving mechanism and a disinfection and sterilization mechanism, wherein the culture dish moving mechanism, the inoculation pore plate moving mechanism and the disinfection and sterilization mechanism are sequentially distributed along the rotating direction of the turntable, the culture dish moving mechanism is arranged on a bottom plate and is used for driving the culture dish to move, the inoculation pore plate moving mechanism is arranged on the bottom plate and is used for driving the inoculation pore plate to move, and the disinfection and sterilization mechanism is arranged on the bottom plate and is used for disinfecting and sterilizing the needle picking device; the bottom plate is also provided with an image acquisition mechanism for acquiring images of colonies in the culture dish.
Above-mentioned prior art moves through culture dish moving mechanism control culture dish, realizes that a week can carry out the work such as picking, inoculation, sterilization many times, but because the position of bacterial colony in the culture dish is random, there is partial bacterial colony to be in culture dish marginal area, thereby can cut the culture dish to incline when generally artificially picking and pick the bacterial colony that is in marginal area, this bacterial colony of prior art picks the appearance and picks the appearance though can carry out many times bacterial colony and pick, but because the culture dish is in keeping flat setting, can not accomplish abundant picking when partial bacterial colony is picked, can appear even unable condition of picking, need solve this kind of equipment of picking that can fully pick the bacterial colony in the culture dish.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model aims to provide an automatic device for picking single bacteria, which has the effect of accurately and effectively picking bacterial colonies in a culture dish.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
an automation equipment for single fungus is chosen, includes the organism, be provided with in the organism and choose the cavity, its characterized in that: the utility model provides a test tube rack, including the cavity of picking, the cavity is provided with tray mechanism, pick mechanism and test-tube rack in, tray mechanism includes tray subassembly and adjusting part, adjusting part connects tray subassembly and organism to be used for adjusting the angle of tray subassembly, the tray subassembly is used for placing the culture dish of cultivateing the bacterial colony, pick the mechanism including picking subassembly and recognition element, recognition element is used for recognizing the position of bacterial colony in the culture dish, pick the subassembly and be used for picking the bacterial colony in the culture dish, the test-tube rack is arranged in one side of picking the mechanism, the test-tube rack is used for placing and collects the test tube, it is used for collecting the bacterial colony after picking the bacterial colony to pick the mechanism to collect the test tube.
As a further improvement of the utility model, the dish supporting component comprises a bottom support, clamping jaws and an elastic piece, wherein a plurality of abdication grooves are formed in the side wall of the bottom support along the circumferential direction, clamping jaws are respectively arranged in any abdication groove, the clamping jaws are connected with the abdication grooves in a sliding manner, and the elastic piece is used for connecting the clamping jaws with the abdication grooves and is arranged along the radial direction of the bottom support.
As a further improvement of the utility model, the end of the clamping jaw away from the bottom wall of the collet is provided with a tongue piece.
As a further improvement of the utility model, the adjusting component comprises a rotary air cylinder and an angle air cylinder, wherein the rotary air cylinder is arranged in the machine body and is connected with the collet, the rotary air cylinder is used for driving the collet to circumferentially rotate, the bottom wall of the collet is provided with a ring groove, the ring groove is internally and slidably connected with a hinge seat, the two angle air cylinders are symmetrically arranged along the collet and are respectively hinged with the hinge seat, and the angle air cylinder is used for adjusting the tilting angle of the culture dish.
As a further improvement of the utility model, the picking assembly comprises a mechanical arm and a picking head, wherein the machine body is provided with a driving box, the mechanical arm is arranged on the driving box, the driving box is used for controlling the mechanical arm to move, the arm end of the mechanical arm is provided with the picking head, and the picking head is used for picking single bacterial colonies.
As a further improvement of the utility model, the picking assembly comprises a mechanical arm and a picking head, wherein the machine body is provided with a driving box, the mechanical arm is arranged on the driving box, the driving box is used for controlling the mechanical arm to move, the arm end of the mechanical arm is provided with the picking head, and the picking head is used for picking single bacterial colonies.
As a further improvement of the utility model, the test tube rack comprises a rotating seat and a rack body, the rotating seat is connected with the rack body and the machine body, the rack body is provided with an annular clamping area, and the collecting test tube is placed in the rack body.
As a further improvement of the utility model, the machine body is also provided with a cover body for covering the picking chamber.
The utility model has the beneficial effects that: the support is carried out to the culture dish through the support dish subassembly that sets up among the tray mechanism, adjusts the angle of culture dish under the effect of adjusting component for the bacterial colony homoenergetic that is arranged in different positions in the culture dish is adjusted to suitable angle, so that the recognition element in choosing the mechanism discerns the bacterial colony and then controls and choose the subassembly to carry out accurate choosing, thereby place the bacterial colony after choosing in the collection test tube on the test-tube rack, realized can fully choosing the effect of bacterial colony in the culture dish.
Drawings
FIG. 1 is a schematic perspective view of a structure embodying the present utility model;
FIG. 2 is a schematic diagram of a structure within a picking chamber;
FIG. 3 is a schematic diagram of a structure embodying a tray mechanism;
fig. 4 is a schematic diagram of a structure embodying an adjustment assembly.
Reference numerals: 1. a body; 11. a cover body; 2. a support dish assembly; 21. a bottom support; 22. a clamping jaw; 23. an elastic member; 24. a tongue piece; 25. a ring groove; 3. an adjustment assembly; 31. a rotary cylinder; 32. an angle cylinder; 4. picking up the component; 41. a drive box; 42. a mechanical arm; 43. picking the head; 5. a test tube rack; 51. a rotating seat; 52. a frame body.
Detailed Description
The utility model will now be described in further detail with reference to the drawings and examples. Wherein like parts are designated by like reference numerals. It should be noted that the words "front", "back", "left", "right", "upper" and "lower" used in the following description refer to directions in the drawings, and the words "bottom" and "top", "inner" and "outer" refer to directions toward or away from, respectively, the geometric center of a particular component.
Referring to fig. 1 to 4, an embodiment of an automatic device for picking single bacteria according to the present utility model includes a machine body 1, a picking chamber is disposed in the machine body 1, and a cover 11 for covering the picking chamber is further disposed on the machine body 1, so that bacteria in the outside air are unlikely to pollute the bacteria in the culture dish when picking colonies in the picking chamber. The novel test tube rack comprises a tray mechanism, a picking mechanism and a test tube rack 5, wherein the tray mechanism comprises a tray assembly 2 and an adjusting assembly 3, the adjusting assembly 3 is connected with the tray assembly 2 and a machine body 1 and is used for adjusting the angle of the tray assembly 2, the tray assembly 2 is used for placing a culture dish for culturing colonies, the picking mechanism comprises a picking assembly 4 and a recognition unit, the recognition unit is used for recognizing the positions of the colonies in the culture dish, the picking assembly 4 is used for picking the colonies in the culture dish, the test tube rack 5 is arranged on one side of the picking mechanism, the test tube rack 5 is used for placing a collecting test tube, and the collecting test tube is used for collecting the colonies after the picking mechanism picks the colonies.
The dish support assembly 2 comprises a bottom support 21, clamping jaws 22 and an elastic piece 23, a plurality of abdicating grooves are formed in the side wall of the bottom support 21 along the circumferential direction, clamping jaws 22 are respectively arranged in any one of the abdicating grooves, the clamping jaws 22 are connected with the abdicating grooves in a sliding mode, the elastic piece 23 is used for connecting the clamping jaws 22 and the abdicating grooves and is arranged along the radial direction of the bottom support 21, the elastic piece 23 is selected as a spring, one end, away from the bottom wall of the bottom support 21, of the clamping jaws 22 is provided with a tongue piece 24, the tongue piece 24 is used for supporting the bottom of the dish when the dish is placed in the bottom support 21, the clamping jaws 22 are pushed to slide along the abdicating grooves and compress the deformation storing force of the elastic piece 23 through pressing the dish, and the clamping jaws 22 clamp the dish under the action of the elastic piece 23 when the dish is pressed into the bottom support 21, so that the dish is not easy to fall off from the bottom support 21.
The adjusting component 3 comprises a rotary cylinder 31 and angle cylinders 32, wherein the rotary cylinder 31 is arranged in the machine body 1 and is connected with the collet 21, the rotary cylinder 31 is used for driving the collet 21 to rotate circumferentially, the bottom wall of the collet 21 is provided with a ring groove 25, the ring groove 25 is internally and slidably connected with a hinge seat, the angle cylinders 32 are symmetrically arranged into two along the collet 21, the two angle cylinders 32 are respectively hinged with the hinge seat, and the angle cylinders 32 are used for adjusting the tilting angle of the culture dish.
The picking assembly 4 comprises a mechanical arm 42 and a picking head 43, a driving box 41 is arranged on the machine body 1, the mechanical arm 42 is arranged on the driving box 41, the driving box 41 is used for controlling the mechanical arm 42 to move, the picking head 43 is arranged at the arm end of the mechanical arm 42, and the picking head 43 is used for picking single bacterial colonies.
The identification unit comprises a controller and an identification camera, wherein a visual identification system is configured in the controller, the visual identification system is used for controlling the identification camera to identify colonies in the culture dish, and the controller controls the picking assembly 4 to pick based on the visual identification system.
The test tube rack 5 comprises a rotating seat 51 and a rack body 52, the rotating seat 51 is connected with the rack body 52 and the machine body 1, the rack body 52 is formed with an annular clamping area, and the collecting test tubes are placed in the rack body 52.
Working principle and effect:
after pressing the culture dish into the collet 21, the single colony is identified by the identification camera at this time (the identification mode is to identify by using the visual identification system in the prior art, the identification principle is that the prior art is not described herein, reference may be made to the image acquisition mechanism in the background art CN212316118U and the image acquisition mechanism in the prior art CN217809424U, but the reference of the prior art is not limited to the provided patent literature), after identifying the single colony, the rotation and/or tilting of the culture dish is adjusted by the adjusting component 3, so that the culture dish is tilted towards the picking mechanism, and the picking component 4 is convenient for picking the single colony. When picking, the angle of the picking head 43 is adjusted through the driving box 41 and the mechanical arm 42 is controlled, the picking head 43 is controlled to pick single bacteria, after picking is finished, the mechanical arm 42 is controlled to drive the picking head 43 to place the picked single bacteria into the collection test tube on the test tube rack 5, so that picking and collection of the single bacteria are finished, and the effect of fully picking bacterial colonies in the culture dish is achieved.
The above description is only a preferred embodiment of the present utility model, and the protection scope of the present utility model is not limited to the above examples, and all technical solutions belonging to the concept of the present utility model belong to the protection scope of the present utility model. It should be noted that modifications and adaptations to the present utility model may occur to one skilled in the art without departing from the principles of the present utility model and are intended to be within the scope of the present utility model.

Claims (8)

1. An automation device for picking single bacteria comprises a machine body (1), wherein a picking cavity is arranged in the machine body (1), and the automation device is characterized in that: the utility model provides a pick up be provided with tray mechanism in the cavity, pick mechanism and test-tube rack (5), tray mechanism includes support dish subassembly (2) and adjusting part (3), adjusting part (3) connect support dish subassembly (2) and organism (1) for adjust the angle of support dish subassembly (2), support dish subassembly (2) are used for placing the culture dish of cultivateing the bacterial colony, pick the mechanism including picking subassembly (4) and recognition element, recognition element is used for recognizing the position of bacterial colony in the culture dish, pick the bacterial colony in subassembly (4) be used for picking the culture dish, test-tube rack (5) set up in one side of picking the mechanism, test-tube rack (5) are used for placing and collect the test tube, collect the test tube and be used for picking the mechanism and gathering the bacterial colony after picking the bacterial colony.
2. An automated apparatus for single-fungus picking as defined in claim 1, wherein: the support dish assembly (2) comprises a bottom support (21), clamping jaws (22) and an elastic piece (23), a plurality of abdicating grooves are formed in the side wall of the bottom support (21) along the circumferential direction, clamping jaws (22) are respectively arranged in any abdicating groove, the clamping jaws (22) are connected with the abdicating grooves in a sliding mode, and the elastic piece (23) is used for connecting the clamping jaws (22) and the abdicating grooves and is arranged along the radial direction of the bottom support (21).
3. An automated apparatus for single-fungus picking as defined in claim 2, wherein: a tongue piece (24) is arranged at one end of the clamping jaw (22) away from the bottom wall of the collet (21).
4. An automated apparatus for single-fungus picking as defined in claim 3, wherein: the adjusting component (3) comprises a rotary cylinder (31) and an angle cylinder (32), wherein the rotary cylinder (31) is arranged in the machine body (1) and is connected with the collet (21), the rotary cylinder (31) is used for driving the collet (21) to rotate circumferentially, the bottom wall of the collet (21) is provided with a ring groove (25), the ring groove (25) is internally and slidably connected with a hinge seat, the angle cylinder (32) is symmetrically arranged into two along the collet (21), the two angle cylinders (32) are hinged with the hinge seat respectively, and the angle cylinder (32) is used for adjusting the tilting angle of the culture dish.
5. An automated apparatus for single-fungus picking as defined in claim 4 wherein: the picking assembly (4) comprises a mechanical arm (42) and a picking head (43), a driving box (41) is arranged on the machine body (1), the mechanical arm (42) is arranged on the driving box (41), the driving box (41) is used for controlling the mechanical arm (42) to move, the picking head (43) is arranged at the arm end of the mechanical arm (42), and the picking head (43) is used for picking single bacterial colonies.
6. An automated apparatus for single-fungus picking as defined in claim 5, wherein: the identification unit comprises a controller and an identification camera, wherein a visual identification system is configured in the controller and used for controlling the identification camera to identify colonies in the culture dish, and the controller controls the picking assembly (4) to pick based on the visual identification system.
7. An automated apparatus for single-fungus picking as defined in claim 6, wherein: test-tube rack (5) are including rotating seat (51) and support body (52), rotate seat (51) connection support body (52) and organism (1), support body (52) are formed with annular clamping area, collect test tube and place in support body (52).
8. An automated apparatus for single-fungus picking as defined in claim 7, wherein: the machine body (1) is also provided with a cover body (11) for covering the picking cavity.
CN202223264833.8U 2023-05-29 2023-05-29 Automatic equipment for picking single bacteria Active CN219297467U (en)

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CN202223264833.8U CN219297467U (en) 2023-05-29 2023-05-29 Automatic equipment for picking single bacteria

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Application Number Priority Date Filing Date Title
CN202223264833.8U CN219297467U (en) 2023-05-29 2023-05-29 Automatic equipment for picking single bacteria

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CN219297467U true CN219297467U (en) 2023-07-04

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904302A (en) * 2023-07-27 2023-10-20 耐优生物技术(上海)有限公司 Colony picking device and colony picking method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904302A (en) * 2023-07-27 2023-10-20 耐优生物技术(上海)有限公司 Colony picking device and colony picking method

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