CN110903959A - Microbial community automatic counting device - Google Patents
Microbial community automatic counting device Download PDFInfo
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- CN110903959A CN110903959A CN201911116440.2A CN201911116440A CN110903959A CN 110903959 A CN110903959 A CN 110903959A CN 201911116440 A CN201911116440 A CN 201911116440A CN 110903959 A CN110903959 A CN 110903959A
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- 230000000813 microbial effect Effects 0.000 title claims description 19
- 238000001514 detection method Methods 0.000 claims abstract description 21
- 244000005700 microbiome Species 0.000 claims abstract description 12
- 238000012360 testing method Methods 0.000 claims abstract description 8
- 230000032258 transport Effects 0.000 claims 2
- 241000233866 Fungi Species 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 238000011835 investigation Methods 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The application discloses microorganism bacterial colony automatic counting device, including examining test table, one side of examining test table is provided with first cylinder, first cylinder is close to the one end extension of examining test table and has first air pressure pole, the other end fixedly connected with clamping device of first air pressure pole, the both sides that are close to clamping device of first cylinder are provided with first support column and second support column respectively, the first bracing piece of upper end fixedly connected with of first support column, the other end fixedly connected with camera unit of first bracing piece, the upper end of second support column is provided with the second bracing piece, the other end of second bracing piece just is at camera unit's below fixedly connected with light filling device, the opposite side of examining test table is provided with the combination cylinder. In this application, adopt full automatic culture dish detection device, help raising the efficiency, reduce the error rate of manual investigation and reduce artificial intensity of labour.
Description
Technical Field
The application relates to the field of microbial culture detection, in particular to an automatic microbial colony counting device.
Background
The automatic counting device for microbial colonies is developed for the fields of biology, medical treatment and the like, and can improve the detection efficiency and accuracy of a culture dish.
At present among the testing process of biological culture dish, mainly through illumination culture dish bacterial colony and take a picture and preserve the image, current equipment is that the manual work is put the culture dish one by one and is put experiment illumination department then gather the image, and speed is very slow and make mistakes easily like this. Meanwhile, the artificial detection is adopted, so that new microorganisms are easily brought into the culture dish, and the culture result of the batch is damaged.
Disclosure of Invention
The application aims to solve the defects in the prior art and provides an automatic counting device for microbial colonies.
In order to achieve the purpose, the following technical scheme is adopted in the application: an automatic counting device for microbial colonies comprises a detection table, wherein a first air cylinder is arranged on one side of the detection table, a first air pressure rod extends from one end, close to the detection table, of the first air cylinder, the other end of the first air pressure rod is fixedly connected with a clamping device, a first supporting column and a second supporting column are respectively arranged on two sides, close to the clamping device, of the first air cylinder, a first supporting rod is fixedly connected to the upper end of the first supporting rod, a camera shooting unit is fixedly connected to the other end of the first supporting rod and connected to an image recognition device, and the image recognition device can realize automatic counting and output of the microbial colonies; the utility model discloses a camera shooting device, including first support column, second support column, camera shooting unit, third pneumatic rod and fourth pneumatic rod, the upper end of second support column is provided with the second bracing piece, the other end of second bracing piece and the below fixedly connected with light filling device at camera shooting unit, the opposite side of examining test table is provided with the combination cylinder, the both sides of combination cylinder have all extended third pneumatic rod and fourth pneumatic rod, the equal fixedly connected with clamping device of the other end of third pneumatic rod and fourth pneumatic rod, the clamping device department of third pneumatic rod and fourth pneumatic rod all is provided with stacks the platform.
As a further description of the above technical solution:
the clamping device comprises a sixth air cylinder, a sixth air pressure rod is arranged on the sixth air cylinder in a downward extending mode, a fifth air cylinder is fixedly connected to the lower end of the sixth air pressure rod, and a hand grip is arranged on the side wall of the fifth air cylinder.
As a further description of the above technical solution:
the stacking platform comprises a third supporting column, the upper end of the third supporting column is fixedly connected with a placing table, a first motor is fixedly connected to one side of the bottom of the placing table, a first lead screw extends to one side of the first motor, and the lower end of a push plate and the lower end of the push plate, which are arranged at the upper end of the placing table, penetrate through the placing table and are in threaded connection with the first lead screw.
As a further description of the above technical solution:
the lateral wall of third support column is at the opposite side below fixedly connected with second motor of putting the platform, the second motor upwards extends there is the second lead screw, the upper end threaded connection of second lead screw has the pole of lifting, the lateral wall of third support column is sliding connection at the upper end fixedly connected with fixed block and the fixed block of second motor and the pole of lifting.
As a further description of the above technical solution:
the combined cylinder comprises a third cylinder and a fourth cylinder, the third cylinder is arranged below the fourth cylinder, a third air pressure rod extends towards one side of the third cylinder, and a fourth air pressure rod extends towards the other side of the fourth cylinder.
As a further description of the above technical solution:
detect one side fixedly connected with third motor of platform, the extension of third motor has the third lead screw, the upper end of detecting the platform is provided with the lower extreme and the third lead screw threaded connection who transfer the platform and transfer to the platform.
As a further description of the above technical solution:
the lateral wall fixedly connected with second cylinder of second support column, second cylinder downwardly extending has second atmospheric pressure pole, the lower extreme fixedly connected with second bracing piece of second atmospheric pressure pole.
As a further description of the above technical solution:
the counting device is arranged in the closed detection cabinet.
As a further description of the above technical solution:
the first motor, the second motor and the third motor are servo stepping motors.
As a further description of the above technical solution:
application of counting device in counting microbial colonies
The application has the following beneficial effects:
this application adopts start-up and electronic full-automatic control mode, can improve the efficiency and the accuracy of the detection of culture dish, can reduce artificial intensity of labour, and the chance of artifical direct contact with the culture dish reduces simultaneously, breeds the risk of non-target bacterial colony in the reduction culture dish.
Drawings
FIG. 1 is a top view of an automatic counting device for microbial colonies proposed in the present application;
FIG. 2 is a schematic view of a clamping device of an automatic counting device for microbial colonies proposed in the present application;
FIG. 3 is a schematic diagram of a stacking platform of the automatic counting apparatus for microbial colonies provided by the present application;
FIG. 4 is a schematic view of a combination cylinder of the automatic counting device for microbial colonies provided by the present application;
FIG. 5 is a schematic view of a detection platform of an automatic counting device for microbial colonies provided by the present application;
fig. 6 is a schematic view of a light supplementing and lifting local structure of an automatic counting device for microbial colonies provided by the present application.
Illustration of the drawings:
1. stacking the platform; 2. a detection table; 3. a first support bar; 4. a first support column; 5. a clamping device; 6. a first pneumatic rod; 7. a first cylinder; 8. a second support column; 9. a second cylinder; 10. a second support bar; 11. a third pneumatic rod; 12. a light supplement device; 13. an image pickup unit; 14. a fourth pneumatic rod; 15. a gripper; 16. a fifth cylinder; 17. a sixth pneumatic rod; 18. a sixth cylinder; 19. a third support column; 20. a first motor; 21. pushing the plate; 22. a first lead screw; 23. a placing table; 24. a second motor; 25. a second lead screw; 26. a fixed block; 27. a lifting rod; 28. a third cylinder; 29. a fourth cylinder; 30. a third lead screw; 31. a transfer table; 32. a third motor; 33. a second pneumatic rod; 34. and (4) combining cylinders.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present application; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
Referring to fig. 1-6, the present application provides one embodiment: an automatic counting device for microbial colonies comprises a detection table 2, wherein a first air cylinder 7 is arranged on one side of the detection table 2, a first air pressure rod 6 extends from one end, close to the detection table 2, of the first air cylinder 7, a clamping device 5 is fixedly connected to the other end of the first air pressure rod 6, a first supporting column 4 and a second supporting column 8 are respectively arranged on two sides, close to the clamping device 5, of the first air cylinder 7, a first supporting rod 3 is fixedly connected to the upper end of the first supporting column 4, a camera unit 13 is fixedly connected to the other end of the first supporting rod 3, the camera unit 13 is connected to an image recognition device, the image recognition device can realize automatic counting and outputting of microbial colonies, a second supporting rod 10 is arranged on the upper end of the second supporting column 8, a light supplement device 12 is fixedly connected to the other end of the second supporting rod 10 below the camera unit 13, and a combined air cylinder 34 is arranged on the, the third pneumatic rod 11 and the fourth pneumatic rod 14 extend from two sides of the combined cylinder 34, the other ends of the third pneumatic rod 11 and the fourth pneumatic rod 14 are fixedly connected with the clamping device 5, and the stacking platform 1 is arranged at the clamping device 5 of the third pneumatic rod 11 and the fourth pneumatic rod 14.
The clamping device 5 comprises a sixth air cylinder 18, a sixth air pressure rod 17 extends downwards from the sixth air cylinder 18, a fifth air cylinder 16 is fixedly connected to the lower end of the sixth air pressure rod 17, a hand grip 15 is arranged on the side wall of the fifth air cylinder 16, the stacking platform 1 comprises a third supporting column 19, a placing table 23 is fixedly connected to the upper end of the third supporting column 19, a first motor 20 is fixedly connected to one side of the bottom of the placing table 23, a first lead screw 22 extends towards one side of the first motor 20, a push plate 21 is arranged at the upper end of the placing table 23, the lower end of the push plate 21 penetrates through the placing table 23 and is in threaded connection with the first lead screw 22, a second motor 24 is fixedly connected to the side wall of the third supporting column 19 below the other side of the placing table 23, a second lead screw 25 extends upwards from the second motor 24, a lifting rod 27 is in threaded connection with the upper end of the second lead screw 25, a fixing block 26 is fixedly connected to the side wall of the third supporting column 19 at, the combined cylinder 34 comprises a third cylinder 28 and a fourth cylinder 29, the third cylinder 28 is arranged below the fourth cylinder 29, the third cylinder 28 extends to one side to form a third pneumatic rod 11, the fourth cylinder 29 extends to the other side to form a fourth pneumatic rod 14, a third motor 32 is fixedly connected to one side of the detection table 2, a third lead screw 30 is arranged on the third motor 32 in an extending mode, a conveying table 31 is arranged at the upper end of the detection table 2, the lower end of the conveying table 31 is in threaded connection with the third lead screw 30, a second cylinder 9 is fixedly connected to the side wall of the second supporting column 8, a second pneumatic rod 33 extends downwards from the second cylinder 9, and a second supporting rod 10 is fixedly connected to the lower end of the second pneumatic rod 33. In order to avoid the pollution of the external environment, the whole counting device can be arranged in a closed detection cabinet (not shown), and the motor is preferably a servo stepping motor.
The working principle is as follows: when the device is used, the stacking platform 1 prevents a culture vessel, the first motor 20 is started, the first lead screw 22 rotates, the push plate 21 pushes the culture vessel to the upper part of the lifting rod 27, the second motor 24 is started, the second lead screw 25 rotates, the lifting rod 27 lifts to lift the height of the culture vessel, the fourth cylinder 29 is inflated, the fourth air pressure rod 14 extends to the position of the culture vessel, the sixth cylinder 18 is inflated, the fifth cylinder 16 descends and has the same height with the culture vessel, the fifth cylinder 16 is inflated, the gripper 15 clamps the culture vessel, the sixth cylinder 18 is deflated, the fifth cylinder 16 ascends, the fourth cylinder 29 is deflated, the fourth air pressure rod 14 retracts to the detection platform 2, the third motor 32 is started, the third lead screw 30 rotates, the conveying platform 31 reaches the lower part of the culture vessel, the sixth cylinder 18 is inflated, the fifth cylinder 16 descends and the fifth cylinder 16 is deflated, the gripper 15 loosens the culture vessel and places the culture vessel on the conveying platform 31, the third motor 32 is started, the third lead screw 30 rotates, the conveying table 31 reaches the position right below the camera shooting unit 13 and the light supplementing device 12, the first air cylinder 7 is inflated, the first air pressure rod 6 extends out, the clamping device 5 at the front end opens the upper cover of the spleen gas sample vessel and retracts, the second air cylinder 9 is inflated, the light supplementing device 12 descends to improve the brightness, the camera shooting unit 13 works, and the culture vessel is covered and conveyed to the stacking platform 1 on the other side in the same mode. The image recognition device connected to the imaging unit 13, for example, a computer, has image recognition software built therein, and can recognize and output the microbial colonies by automatic counting.
Finally, it should be noted that: although the present application has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the present application.
Claims (10)
1. The utility model provides a microorganism fungus colony automatic counting device, is including examining test table (2), its characterized in that: a first air cylinder (7) is arranged on one side of the detection table (2), a first air pressure rod (6) extends from one end, close to the detection table (2), of the first air cylinder (7), a clamping device (5) is fixedly connected to the other end of the first air pressure rod (6), a first supporting column (4) and a second supporting column (8) are respectively arranged on two sides, close to the clamping device (5), of the first air cylinder (7), a first supporting rod (3) is fixedly connected to the upper end of the first supporting column (4), a camera unit (13) is fixedly connected to the other end of the first supporting rod (3), the camera unit (13) is connected to an image recognition device, and the image recognition device can realize automatic counting and output of microbial colonies; the upper end of second support column (8) is provided with second bracing piece (10), the other end of second bracing piece (10) and in below fixedly connected with light filling device (12) of camera unit (13), the opposite side of examining test table (2) is provided with combination cylinder (34), the both sides of combination cylinder (34) extend respectively and have third pneumatic rod (11) and fourth pneumatic rod (14), the equal fixedly connected with clamping device (5) of the other end of third pneumatic rod (11) and fourth pneumatic rod (14), clamping device (5) department of third pneumatic rod (11) and fourth pneumatic rod (14) all is provided with stacks platform (1).
2. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: clamping device (5) include sixth cylinder (18), the downwardly extending of sixth cylinder (18) has the lower extreme fixedly connected with fifth cylinder (16) of sixth pneumatic rod (17) and sixth pneumatic rod (17), the lateral wall of fifth cylinder (16) is provided with tongs (15).
3. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: stacking platform (1) includes third support column (19), the upper end fixedly connected with of third support column (19) puts platform (23), the first motor (20) of bottom one side fixedly connected with of putting platform (23), first motor (20) extend to one side has first lead screw (22), the lower extreme that the upper end of putting platform (23) was provided with push pedal (21) and push pedal (21) runs through putting platform (23) and first lead screw (22) threaded connection.
4. The apparatus for automatically counting colonies of microorganisms according to claim 3, wherein: the lateral wall of third support column (19) is at opposite side below fixedly connected with second motor (24) of putting platform (23), second motor (24) upwards extend has second lead screw (25), the upper end threaded connection of second lead screw (25) has lifting rod (27), the lateral wall of third support column (19) is sliding connection at the upper end fixedly connected with fixed block (26) of second motor (24) and fixed block (26) and lifting rod (27).
5. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: the combined cylinder (34) comprises a third cylinder (28) and a fourth cylinder (29), the third cylinder (28) is arranged below the fourth cylinder (29), a third air pressure rod (11) extends towards one side of the third cylinder (28), and a fourth air pressure rod (14) extends towards the other side of the fourth cylinder (29).
6. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: detect one side fixedly connected with third motor (32) of platform (2), the extension of third motor (32) has third lead screw (30), the upper end of detecting platform (2) is provided with the lower extreme and the third lead screw (30) threaded connection who transports platform (31) and transport platform (31).
7. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: the lateral wall fixedly connected with second cylinder (9) of second support column (8), second cylinder (9) downwardly extending has second pneumatic rod (33), the lower extreme fixedly connected with second bracing piece (10) of second pneumatic rod (33).
8. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: the counting device is arranged in the closed detection cabinet.
9. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: the first motor (20), the second motor (24) and the third motor (32) are servo stepper motors.
10. The apparatus for automatically counting colonies of microorganisms according to claim 1, wherein: the counting device is applied to counting microbial colonies.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911116440.2A CN110903959A (en) | 2019-11-15 | 2019-11-15 | Microbial community automatic counting device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911116440.2A CN110903959A (en) | 2019-11-15 | 2019-11-15 | Microbial community automatic counting device |
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| CN110903959A true CN110903959A (en) | 2020-03-24 |
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| CN201911116440.2A Pending CN110903959A (en) | 2019-11-15 | 2019-11-15 | Microbial community automatic counting device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112899138A (en) * | 2021-01-11 | 2021-06-04 | 浙江省海洋水产研究所 | Device and method for determining microbial colony number |
| CN112899148A (en) * | 2021-01-11 | 2021-06-04 | 浙江省海洋水产研究所 | Microbial colony count measuring device and measuring method |
| CN113980800A (en) * | 2021-11-25 | 2022-01-28 | 深圳零一生命科技有限责任公司 | Microbial colony counter and counting method thereof |
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Application publication date: 20200324 |