CN219168460U - Combined sample adding device - Google Patents

Abstract

The utility model discloses a combined sample adding device, which belongs to the field of medical equipment and comprises a combined plate, wherein the combined plate comprises a sample plate and a containing plate, the containing plate is arranged on the sample plate, the sample plate is provided with a sample containing part, the containing plate is provided with a plurality of containing holes and sample injection flow passages, the sample injection flow passages are communicated with the sample containing part and the containing holes, the combined sample adding device also comprises a liquid moving structure, the liquid moving structure is communicated with the plurality of containing holes, the liquid moving structure enables the plurality of containing holes to form negative pressure, liquid samples in the sample containing part respectively flow into each containing hole along the sample injection flow passages, and through the design, the sample can be added to the plurality of containing holes at one time and the mutual mixing pollution of solutions in the containing holes is avoided.

Description

Combined sample adding device

Technical Field

The utility model relates to the field of medical instruments, in particular to a combined sample adding device.

Background

In the biomedical field, there is often a scenario in which a sample needs to be added to each well in a multi-well plate, while communication and mutual contamination between the wells are not allowed. In the prior art, a disposable pipette gun is adopted, and the disposable pipette tips of the pipette gun need to be replaced between sample adding holes, so that time and labor are wasted, and consumption of the disposable pipette tips is increased.

Disclosure of Invention

In order to overcome the defects of the prior art, one of the purposes of the utility model is to provide a combined sample application device which can apply samples to a plurality of containing holes at one time and avoid the mutual mixing pollution of solutions in the containing holes.

One of the purposes of the utility model is realized by adopting the following technical scheme:

the utility model provides a combination formula application of sample device, includes the combination board, the combination board includes sample board and holds the board, hold the board install in the sample board, the sample board is equipped with sample acceptors, it is equipped with a plurality of accommodation holes and advances the appearance runner to hold the board, advance the appearance runner with sample acceptors and accommodation hole intercommunication, combination formula application of sample device still includes the pipetting structure, pipetting structure and a plurality of accommodation hole intercommunication, pipetting structure makes a plurality of accommodation hole forms the negative pressure, liquid sample in the sample acceptors is followed advance the appearance runner and flow into each respectively the accommodation hole.

Further, the number of the sample injection flow passages is the same as that of the containing holes, each sample injection flow passage extends from the bottom of the outer wall of the containing hole to the position close to the top, and the sample injection flow passages are communicated with the inside of the containing hole at the position close to the top of the containing hole.

Further, the sample plate is further provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove, the sample adding groove is communicated with the sample accommodating part through the liquid inlet, and the sample accommodating part is communicated with the waste liquid groove through the overflow port.

Furthermore, the bottom of the sample adding tank is obliquely arranged, and the lowest part of the bottom of the sample adding tank is connected with the bottom of the liquid inlet.

Further, the sample accommodating part is provided with a plurality of sample holes, and the plurality of sample holes are communicated through a main flow channel.

Further, the pipetting structure comprises a gas valve assembly, a piston assembly and a valve core, wherein the gas valve assembly is hermetically arranged on the combination plate, the gas valve assembly is provided with a piston cavity and a pressure changing cavity communicated with the piston cavity, the piston assembly is arranged on the gas valve assembly and stretches into the piston cavity, the valve core comprises a second sliding block, the second sliding block is provided with a sixth through hole, the valve core is arranged in the pressure changing cavity, the second sliding block is abutted to the bottom wall of the pressure changing cavity to enable the pressure changing cavity to be disconnected with the accommodating hole, the piston assembly moves in the piston cavity to enable the pressure in the pressure changing cavity to change, when the pressure in the pressure changing cavity is reduced, pressure difference is formed at two ends of the second sliding block, the second sliding block is separated from the bottom wall of the pressure changing cavity, the pressure changing cavity is communicated with the accommodating hole through the sixth through hole, negative pressure is generated in the accommodating hole, and liquid samples in the sample accommodating part flow into the accommodating hole respectively along the sample injection flow passage.

Further, when the pressure in the pressure changing cavity is increased, the two ends of the first sliding block form a pressure difference, the first sliding block drives the second sliding block to move so that the second sliding block is separated from the bottom wall of the pressure changing cavity, the pressure changing cavity is communicated with the containing hole through the sixth through hole, compressed gas in the pressure changing cavity is injected into the containing hole, and the pipetting structure is separated from the combination plate.

Further, the pipetting structure further comprises an elastic piece, two ends of the elastic piece are respectively abutted against the valve core and the air valve assembly, and the elastic force of the elastic piece enables the second sliding block to be abutted against the bottom wall of the pressure changing cavity.

Further, the air valve assembly is further provided with a third through hole, the third through hole is located between the pressure transformation cavity and the accommodating hole, the sectional area of the third through hole is smaller than the sectional area of the bottom of the pressure transformation cavity, a table top is formed between the third through hole and the bottom of the pressure transformation cavity, and when the second sliding block is in contact with the bottom wall of the pressure transformation cavity, the sixth through hole is in contact with the table top.

Further, the pipetting structure is a vacuum pump.

Compared with the prior art, the combined plate of the combined type sample adding device comprises a sample plate and a containing plate, wherein the containing plate is arranged on the sample plate, the sample plate is provided with a sample containing part, the containing plate is provided with a plurality of containing holes and a sample injection runner, the sample injection runner is communicated with the sample containing part and the containing holes, the combined type sample adding device further comprises a liquid moving structure, the liquid moving structure is communicated with the containing holes, the liquid moving structure enables the containing holes to form negative pressure, liquid samples in the sample containing part respectively flow into each containing hole along the sample injection runner, and through the design, the sample can be added into the containing holes at one time and the mutual mixing pollution of solutions in the containing holes is avoided.

Drawings

FIG. 1 is a perspective view of a combined sample application device according to the present utility model;

FIG. 2 is an exploded view of the combined sample application device of FIG. 1;

FIG. 3 is an exploded view of the compoboard of the compounder-load device of FIG. 2;

FIG. 4 is a perspective view of a sample plate of the composite plate of FIG. 3;

FIG. 5 is a perspective view of a containment plate of the composite plate of FIG. 3;

FIG. 6 is another perspective view of a containment plate of the composite plate of FIG. 3;

FIG. 7 is a perspective cross-sectional view of the composite board of FIG. 3;

FIG. 8 is an enlarged view of the composite board of FIG. 7 at A;

FIG. 9 is an exploded view of a pipetting structure of the combined loading device of FIG. 1;

FIG. 10 is a perspective view of the valve seat of the pipetting structure of FIG. 9;

FIG. 11 is a perspective view of a valve cartridge of the pipetting structure of FIG. 9;

FIG. 12 is a cross-sectional view of the combined sample application device of FIG. 1;

FIG. 13 is a schematic view showing a partial structure of the combined sample application device of FIG. 12.

In the figure: 10. a combination board; 11. a sample plate; 111. a sample adding groove; 112. a liquid inlet; 113. a main flow passage; 114. a sample storage section; 115. an overflow port; 116. a waste liquid tank; 117. a first seal groove; 12. a receiving plate; 121. a receiving hole; 122. a sample injection flow passage; 123. a sample adding port; 124. a first end face; 125. a second end face; 126. a second seal groove; 13. a first seal ring; 20. a pipetting structure; 21. a gas valve assembly; 210. a sealing gasket; 2101. a first through hole; 211. a lower seat; 2110. a first fixing hole; 2111. a second through hole; 2112. a third through hole; 212. a gasket; 2120. a fourth through hole; 2121. a second fixing hole; 213. an upper seat; 2130. a mounting hole; 2132. a fifth through hole; 2133. a third fixing hole; 214. a second seal ring; 215. a piston seat; 2150. a piston chamber; 216. a fixing member; 217. a variable pressure cavity; 22. a piston assembly; 220. an end cap; 221. a piston rod; 23. a valve core; 231. a slide bar; 232. a first slider; 233. a second slider; 2330. a sixth through hole; 24. an elastic member.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1 to 13, the combined loading device of the present utility model includes a combination plate 10 and a pipetting structure 20.

The combined plate 10 comprises a sample plate 11, a receiving plate 12 and a first sealing ring 13.

The sample plate 11 is provided with a sample addition well 111, a liquid inlet 112, a main channel 113, a sample storage section 114, an overflow port 115, a waste liquid tank 116, and a first seal groove 117. The loading slot 111 is located at the end of the sample plate 11. In this embodiment, the sample loading slot 111 is in a long strip shape, the bottom of the sample loading slot 111 is an inclined plane, the lowest part of the inclined plane is connected with the liquid inlet 112, and the liquid inlet 112 is located between the sample loading slot 111 and the sample receiving portion 114. The solution sample enters the sample storage section 114 through the sample addition groove 111 and the liquid inlet 112. In this embodiment, the sample receiving portion 114 is a plurality of cylindrical holes, and the plurality of cylindrical holes are mutually communicated through the main flow channel 113, so that only a small amount of solution sample is required to fill the sample receiving portion 114. In other embodiments, the sample receiving portion 114 may also be a rectangular recess. An overflow port 115 is provided at the other end of the sample receiving portion 114, and when the amount of the added solution sample is excessive, the solution sample overflows from the overflow port 115 to a waste liquid tank 116, maintaining the uniformity of the height of the sample solution. The first seal groove 117 is used for mounting the first seal ring 13, and the first seal ring 13 seals the sample plate 11 from the accommodating plate 12, so that the sample solution in the sample plate 11 is prevented from overflowing through the contact surface.

The accommodating plate 12 is provided with a plurality of accommodating holes 121, a plurality of sample introduction flow passages 122, a sample introduction port 123, a first end surface 124, a second end surface 125, and a second seal groove 126. A plurality of receiving holes 121 are provided on the first end surface 124, each receiving hole 121 extending downward from the first end surface 124. The number of the injection runners 122 is the same as the number of the accommodating holes 121. Each of the sample introduction flow passages 122 extends from the bottom of the outer wall of the accommodating hole 121 to a position near the top of the accommodating hole 121. The injection flow passage 122 communicates with the inside of the accommodating hole 121 near the top of the accommodating hole 121. The loading port 123 is located at the end of the receiving plate 12, and the loading port 123 is used to pour the solution template into the loading slot 111. The first end surface 124 is lower in height than the second end surface 125 to facilitate a contact fit of the first end surface 124 with the lower end surface of the gasket 210 to achieve a tight seal. The second seal groove 126 is used to mount the first seal ring 13, and the first seal ring 13 seals the sample plate 11 from the accommodating plate 12, preventing the sample solution in the sample plate 11 from overflowing through the contact surface.

The receiving plate 12 is attached to the sample plate 11, the receiving hole 121 extends into the sample receiving portion 114, the position of the sample addition port 123 corresponds to the sample addition groove 111, and the first seal ring 13 seals the sample plate 11 from the receiving plate 12.

The pipetting structure 20 comprises a gas valve assembly 21, a piston assembly 22, a valve core 23 and an elastic member 24.

The air valve assembly 21 comprises a sealing gasket 210, a lower seat 211, a gasket 212, an upper seat 213, a plurality of second sealing rings 214, a piston seat 215 and a fixing piece 216 which are sequentially arranged.

The gasket 210 is used to keep the pipetting structure 20 sealed with the deck plate 10 and prevent air leakage between the pipetting structure 20 and the deck plate 10. The gasket 210 is provided with a first through hole 2101, and the first through hole 2101 communicates with the accommodation hole 121 to regulate the air pressure in the accommodation hole 121.

The lower seat 211 is provided with a first fixing hole 2110, a second through hole 2111 and a third through hole 2112, and the first fixing hole 2110 is used for mounting the fixing member 216. The second through hole 2111 is for receiving the second slider 233 of the spool 23. The second through hole 2111 is cylindrical and the third through hole 2112 is also cylindrical, and the diameter of the second through hole 2111 is larger than the diameter of the third through hole 2112. The center line of the second through hole 2111 and the center line of the third through hole 2112 are on the same axis. A mesa is formed between the second through hole 2111 and the third through hole 2112.

The spacer 212 is provided with a fourth through hole 2120 and a second fixing hole 2121. The fourth through hole 2120 is for gas flow and the slide bar 231 of the valve spool 23 is extended. The second fixing hole 2121 is used for mounting the fixing 216.

The upper seat 213 is provided with a mounting hole 2130, a fifth through hole 2132, and a third fixing hole 2133. The mounting hole 2130 is used to mount the spool 23. The fifth through hole 2132 is for gas flow. The third fixing hole 2133 is for the fixing piece 216.

The number of the second seal rings 214 is plural, and the plural second seal rings 214 are used to seal the piston rod 221.

The piston seat 215 is provided with a piston cavity 2150.

The fixing member 216 is used for connecting and fixing the lower seat 211, the gasket 212 and the upper seat 213. Specifically, the fixing member 216 is a screw.

The fixing member 216 extends into the first fixing hole 2110 of the lower seat 211, the second fixing hole 2121 of the spacer 212, and the third fixing hole 2133 of the upper seat 213, so that the lower seat 211, the spacer 212, and the upper seat 213 are sequentially fixed. At this time, the fifth through hole 2132, the fourth through hole 2120, and the second through hole 2111 together form the transformer chamber 217.

The piston assembly 22 includes an end cap 220 and a plurality of piston rods 221. The piston assembly 22 is mounted to the valve assembly 21 and the piston rod 221 extends into the piston chamber 2150.

The valve spool 23 includes a slide bar 231, a first slider 232, and a second slider 233. The first slider 232 and the second slider 233 extend from the slide bar 231. The second slider 233 is located at an end of the spool 23. The second slider 233 is provided with a sixth through hole 2330. The valve spool 23 is installed in the variable pressure chamber 217, and the second slider 233 partially abuts against the bottom wall of the variable pressure chamber 217 (the lands of the second through hole 2111 and the third through hole 2112). At this time, the sixth through hole 2330 abuts against the bottom wall of the transformation chamber 217, so that the third through hole 2112 is disconnected from the transformation chamber 217.

The elastic member 24 is sleeved on the sliding rod 231 of the valve core 23, and two ends of the elastic member 24 respectively abut against the upper wall of the mounting hole 2130 of the valve assembly 21 and the first slider 232 of the valve core 23. Specifically, the elastic member 24 is a spring.

When the combined sample application device is used, the pipetting structure 20 is mounted on the assembly plate 10 and the gasket 210 is in contact with the first end surface 124 to realize sealing. The solution sample enters the sample storage section 114 through the sample addition groove 111 and the liquid inlet 112.

The piston assembly 22 is controlled to move up and down regularly by a controller such as an external mechanical arm, and when the piston rod 221 moves up, the piston chamber 2150 sucks air into the pressure changing chamber 217, so that the gas pressure in the pressure changing chamber 217 is reduced. At this time, a pressure difference is formed between the upper and lower ends of the second slider 233, the elastic member 24 is further compressed under the action of pressure, the valve core 23 moves upward, the sixth through hole 2330 communicates the pressure changing chamber 217 and the third through hole 2112 with the accommodating hole 121, and under the action of negative pressure, the pressure changing chamber 217 sucks air in the accommodating hole 121 through the third through hole 2112. Because the air in the accommodating hole 121 is reduced to form negative pressure, a pressure difference is formed at two ends of the liquid level of the solution sample in the sample accommodating part 114, the sample solution is pressed into the accommodating hole 121 through the sample injection flow channel 122 under the action of external atmospheric pressure, the mixture with the medicine is realized, and the amount of the sample solution added into the accommodating hole 121 is controlled by controlling the movement stroke of the piston rod 221.

After the piston rod 221 stops moving, the pressure difference between the two ends of the second slider 233 is reduced, the valve core 23 moves downward under the action of the elastic member 24, and the gas passage between the pressure changing chamber 217 and the receiving hole 121 is closed.

When the piston rod 221 moves downward, the piston chamber 2150 injects air into the variable pressure chamber 217, resulting in an increase in the gas pressure in the variable pressure chamber 217. The upper and lower ends of the first slider 232 are pressure-differential, the elastic member 24 is further compressed under the pressure, the valve core 23 moves upward, and the sixth through hole 2330 communicates the pressure-variable chamber 217 with the receiving hole 121. The compressed gas is injected into the accommodating hole 121 by the pressure of the gas in the pressure-varying chamber 217. Under the action of air pressure, the combined plate 10 is subjected to downward force exerted by the pipetting structure 20, the combined plate 10 is separated from the pipetting structure 20, and the whole pipetting process is completed.

In other embodiments, the pipetting structure 20 is a vacuum pump, and the vacuum pump is used to evacuate the receiving hole 121, thereby completing the pipetting operation from the sample receiving portion 114 to the receiving hole 121.

The combination board 10 and the pipetting structure 20 cooperate in this application, realize that sample solution shifts to the accommodation plate 12 from sample board 11 accurately, saved the loaded down with trivial details process that traditional combination board haplopore was pipetted one by one, avoided the waste that the disposable pipetting gun head of change caused simultaneously.

The foregoing examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present utility model, which are equivalent to the above embodiments according to the essential technology of the present utility model, and these are all included in the protection scope of the present utility model.

Claims (10)

1. The utility model provides a combination formula application of sample device, includes composite board, its characterized in that: the combined plate comprises a sample plate and a containing plate, the containing plate is arranged on the sample plate, the sample plate is provided with a sample containing part, the containing plate is provided with a plurality of containing holes and sample injection flow passages, the sample injection flow passages are communicated with the sample containing part and the containing holes, the combined sample adding device further comprises a pipetting structure, the pipetting structure is communicated with the containing holes, the pipetting structure enables the containing holes to form negative pressure, and liquid samples in the sample containing part flow into each containing hole along the sample injection flow passages respectively.

2. The combination loading device of claim 1, wherein: the number of the sample injection flow passages is the same as that of the containing holes, each sample injection flow passage extends from the bottom of the outer wall of the containing hole to the position close to the top, and the sample injection flow passages are communicated with the inside of the containing hole at the position close to the top of the containing hole.

3. The combination loading device of claim 1, wherein: the sample plate is also provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove, wherein the sample adding groove is communicated with the sample accommodating part through the liquid inlet, and the sample accommodating part is communicated with the waste liquid groove through the overflow port.

4. A combined sample application device according to claim 3, wherein: the bottom of the sample adding tank is obliquely arranged, and the lowest part of the bottom of the sample adding tank is connected with the bottom of the liquid inlet.

5. The combination loading device of claim 1, wherein: the sample accommodating part is provided with a plurality of sample holes, and a plurality of sample holes are communicated through a main flow channel.

6. The combined sample application device according to any one of claims 1 to 5 wherein: the liquid transferring structure comprises a gas valve assembly, a piston assembly and a valve core, wherein the gas valve assembly is hermetically arranged on the combined plate, the gas valve assembly is provided with a piston cavity and a pressure changing cavity communicated with the piston cavity, the piston assembly is arranged on the gas valve assembly and stretches into the piston cavity, the valve core comprises a second sliding block, the second sliding block is provided with a sixth through hole, the valve core is arranged in the pressure changing cavity, the second sliding block is abutted to the bottom wall of the pressure changing cavity to enable the pressure changing cavity to be disconnected with the accommodating hole, the piston assembly moves in the piston cavity to enable the pressure in the pressure changing cavity to change, when the pressure in the pressure changing cavity is reduced, pressure difference is formed at two ends of the second sliding block, the second sliding block is separated from the bottom wall of the pressure changing cavity, the pressure changing cavity is communicated with the accommodating hole through the sixth through hole, the accommodating hole generates negative pressure, and liquid samples in the sample accommodating part flow into the accommodating hole respectively along the sample feeding flow channels.

7. The combination loading device of claim 6, wherein: the valve core further comprises a first sliding block, when the pressure in the pressure changing cavity is increased, pressure difference is formed at two ends of the first sliding block, the first sliding block drives the second sliding block to move so that the second sliding block is separated from the bottom wall of the pressure changing cavity, the pressure changing cavity is communicated with the containing hole through the sixth through hole, compressed gas in the pressure changing cavity is injected into the containing hole, and the pipetting structure is separated from the combination plate.

8. The combination loading device of claim 7, wherein: the liquid transferring structure further comprises an elastic piece, two ends of the elastic piece are respectively abutted against the valve core and the air valve assembly, and the elastic force of the elastic piece enables the second sliding block to be abutted against the bottom wall of the pressure changing cavity.

9. The combination loading device of claim 6, wherein: the air valve assembly is further provided with a third through hole, the third through hole is located between the pressure changing cavity and the accommodating hole, the sectional area of the third through hole is smaller than the sectional area of the bottom of the pressure changing cavity, a table top is formed between the third through hole and the bottom of the pressure changing cavity, and when the second sliding block is in contact with the bottom wall of the pressure changing cavity, the sixth through hole is in contact with the table top.

10. The combined sample application device according to any one of claims 1 to 5 wherein: the pipetting structure is a vacuum pump.

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