CN115945232A - Quick application of sample device - Google Patents
Quick application of sample device Download PDFInfo
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- CN115945232A CN115945232A CN202211699831.3A CN202211699831A CN115945232A CN 115945232 A CN115945232 A CN 115945232A CN 202211699831 A CN202211699831 A CN 202211699831A CN 115945232 A CN115945232 A CN 115945232A
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- sample
- liquid
- rapid
- accommodation hole
- adding device
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Abstract
The invention discloses a rapid sample adding device, which belongs to the field of medical instruments and comprises a sample plate and a containing plate, wherein the sample plate is provided with a sample containing part and a plurality of liquid guide needles positioned in the sample containing part, the containing plate comprises a main body, a first sealing film and a second sealing film, the main body is provided with a plurality of containing holes and a sample introduction flow channel, the sample introduction flow channel is communicated with the containing holes, the end parts of the containing holes are sealed by the first sealing film, the sample introduction flow channel is sealed by the second sealing film, negative pressure is formed in the containing holes and the sample introduction flow channel communicated with the containing holes, when the rapid sample adding device is used, the containing plate is arranged on the sample plate, and the liquid guide needles pierce the second sealing film to enable liquid samples in the sample containing part to respectively flow into the containing holes along the sample introduction flow channel.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a rapid sample adding device.
Background
In the biomedical field, situations often arise where it is necessary to add a sample to each well in a multiwell plate without allowing communication and contamination between the wells. The disposable pipette tip of the pipette is required to be replaced between the samples of each hole by adopting the disposable pipette gun in the prior art, so that time and labor are wasted, and the consumption of the disposable pipette tip is increased.
Disclosure of Invention
In order to overcome the defects of the prior art, an object of the present invention is to provide a rapid sample adding device capable of adding samples to a plurality of receiving holes at a time and preventing the solutions in the receiving holes from being mixed and polluted.
One of the purposes of the invention is realized by adopting the following technical scheme:
the utility model provides a quick application of sample device, includes the sample board and holds the board, the sample board is equipped with sample holding portion and a plurality of being located the liquid needle that draws of sample holding portion, it includes main part, first seal membrane and second seal membrane to hold the board, the main part is equipped with a plurality of accommodation holes and advances the kind runner, advance the kind runner with the accommodation hole intercommunication, first seal membrane is sealed the accommodation hole tip, the second seal membrane is sealed advance the kind runner, make the accommodation hole and with form the negative pressure in the kind runner of accommodation hole intercommunication, when quick application of sample device used, hold the board install in the sample board, draw the liquid needle puncture the second seal membrane makes liquid sample in the sample holding portion is followed advance the kind runner and flow in each respectively the accommodation hole.
Furthermore, the number of the sample injection flow channels is the same as that of the accommodating holes, each sample injection flow channel extends from the bottom of the outer wall of the accommodating hole to a position close to the top, and the sample injection flow channels are communicated with the inside of the accommodating holes at the positions, close to the tops, of the accommodating holes.
Furthermore, the inner wall of accommodation hole is equipped with protruding and sunken, protruding with sunken corresponding the setting and closing together form advance a kind runner, advance a kind runner's top with the accommodation hole intercommunication.
Further, the accommodation hole is hollow structure, the accommodation hole includes the bottom surface, advance the kind runner and extend to the bottom surface and be in the bottom surface forms the through-hole, the second seal membrane is sealed the through-hole.
Further, the liquid guide needle is of a hollow structure.
Furthermore, draw the liquid needle and be equipped with the breach, the breach extends along draw the extending direction of liquid needle, draw the liquid needle inside through the breach with sample portion intercommunication.
Further, the end part of the liquid guide needle is conical.
Furthermore, the sample plate is also provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove, wherein the sample adding groove is communicated with the sample accommodating part through the liquid inlet, and the sample accommodating part is communicated with the waste liquid groove through the overflow port.
Furthermore, the bottom of the sample adding slot is obliquely arranged, and the lowest part of the bottom of the sample adding slot is connected with the bottom of the liquid inlet.
Further, the sample holding part is provided with a plurality of sample holes, and the plurality of sample holes are communicated with each other through a main flow channel.
Compared with the prior art, the sample plate of the rapid sample adding device is provided with the sample containing part and the liquid guide needles positioned in the sample containing part, the containing plate comprises a main body, a first sealing film and a second sealing film, the main body is provided with a plurality of containing holes and sample introduction channels, the sample introduction channels are communicated with the containing holes, the ends of the containing holes are sealed by the first sealing film, the sample introduction channels are sealed by the second sealing film, negative pressure is formed in the containing holes and the sample introduction channels communicated with the containing holes, when the rapid sample adding device is used, the containing plate is installed on the sample plate, and the liquid guide needles pierce the second sealing film to enable liquid samples in the sample containing part to respectively flow into the containing holes along the sample introduction channels.
Drawings
FIG. 1 is a perspective view of a rapid sample application device according to the present invention;
FIG. 2 is an exploded view of the rapid sample loading device of FIG. 1;
FIG. 3 is a perspective view of a sample plate of the rapid sample addition device of FIG. 2;
FIG. 4 is a cross-sectional view of the sample plate of FIG. 3;
FIG. 5 is an exploded view of a containment plate of the rapid sample loading device of FIG. 2;
FIG. 6 is a perspective view of the body of the containment plate of FIG. 5;
FIG. 7 is an enlarged view of the body of FIG. 6 at A;
FIG. 8 is another perspective view of the body of FIG. 6;
FIG. 9 is a cross-sectional view of the rapid sample loading device of FIG. 1;
fig. 10 is an enlarged view of the rapid sample loading device of fig. 9 at B.
In the figure: 11. a sample plate; 111. a sample adding slot; 112. a liquid inlet; 113. a main flow channel; 114. a sample holding section; 115. an overflow port; 116. a waste liquid tank; 117. a first seal groove; 118. a liquid introducing needle; 12. a receiving plate; 120. a main body; 1201. an accommodation hole; 12010. an inner wall; 12011. a protrusion; 12012. a top end; 12013. recessing; 12014. a bottom surface; 12015. a through hole; 1202. a sample injection flow channel; 1203. a sample addition port; 1204. a first end face; 1205. a second end face; 1206. a second seal groove; 121. a first sealing film; 122. a second sealing film; 13. and (5) sealing rings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present, secured by intervening elements. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When an element is referred to as being "disposed on" another element, it can be directly disposed on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
As shown in fig. 1 to 10, the rapid sample adding device of the present invention includes a sample plate 11, a containing plate 12, and a sealing ring 13.
The sample plate 11 is provided with a sample addition well 111, a liquid inlet 112, a main channel 113, a sample accommodation part 114, an overflow port 115, a waste liquid tank 116, a first seal groove 117, and a liquid introduction needle 118.
The sample addition slot 111 is located at the end of the sample plate 11. In this embodiment, the sample addition slot 111 is a long strip, the bottom of the sample addition slot 111 is an inclined plane, and the lowest part of the inclined plane is connected with the liquid inlet 112.
The loading port 112 is located between the loading slot 111 and the sample holding part 114. The solution sample enters the sample holding portion 114 through the sample addition slot 111 and the liquid inlet 112. In this embodiment, the sample holding part 114 is a plurality of cylindrical holes, and the plurality of cylindrical holes are communicated with each other through the main flow channel 113, so that only a small amount of the solution sample is required to fill the sample holding part 114. In other embodiments, the sample holder 114 may be a rectangular groove.
The overflow port 115 is located at the other end of the sample holding portion 114, and when the amount of the added solution sample is too large, the solution sample overflows from the overflow port 115 to the waste liquid tank 116, thereby maintaining the uniformity of the sample solution height.
The first sealing groove 117 is used for installing the sealing ring 13, and the sealing ring 13 seals the sample plate 11 and the containing plate 12 to prevent the sample solution in the sample plate 11 from overflowing through the contact surface.
The number of the liquid introducing needles 118 is plural. Specifically, the number of the liquid introduction needles 118 is the same as that of the accommodating holes 1201, so that each liquid introduction needle 118 can correspond to one accommodating hole 1201. The liquid guide needle 118 has a hollow structure, and the liquid guide needle 118 is provided with a notch extending along the extending direction (vertical direction) of the liquid guide needle 118, and the inside of the liquid guide needle 118 is communicated with the sample containing part 114 through the notch. The end of the liquid guiding needle 118 is tapered to facilitate the liquid guiding needle 118 to pierce the second sealing film 122.
The receiving plate 12 includes a main body 120, a first sealing film 121, and a second sealing film 122.
The main body 120 has a plurality of receiving holes 1201, a plurality of sample inlet channels 1202, a sample inlet 1203, a first end 1204, a second end 1205, and a second sealing groove 1206.
The accommodating holes 1201 are disposed on the first end surface 1204, and each accommodating hole 1201 extends downward from the first end surface 1204 and is hollow.
The number of the sample flow paths 1202 is the same as that of the accommodating holes 1201. Each sample flow channel 1202 extends from the bottom of the outer wall of the receiving hole 1201 to the top of the receiving hole 1201. The sample introduction flow channel 1202 communicates with the inside of the accommodation hole 1201 near the top of the accommodation hole 1201. Specifically, the inner wall 12010 of the accommodating hole 1201 is provided with a protrusion 12011 and a recess 12013, the protrusion 12011 and the recess 12013 are correspondingly disposed and folded to form the sample injection channel 1202, and the top 12012 of the sample injection channel 1202 is communicated with the accommodating hole 1201. The accommodating hole 1201 includes a bottom surface 12014, the sample inlet channel 1202 extends to the bottom surface 12014 and forms a through hole 12015 on the bottom surface 12014, and the second sealing film 122 seals the through hole 12015.
The sample addition port 1203 is located at an end of the holding plate 12, and the sample addition port 1203 is used to pour the solution template into the sample addition well 111. The first end face 1204 is lower in height than the second end face 1205, facilitating the mounting of the first sealing film 121 against the first end face 1204, achieving a tight seal of the receiving hole 1201. The second sealing groove 1206 is used for installing a sealing ring 13, and the sealing ring 13 seals the sample plate 11 and the containing plate 12 to prevent the sample solution in the sample plate 11 from overflowing through the contact surface.
When the rapid sample adding device of the present invention is used, the sample plate 11 is separated from the holding plate 12 in the initial state.
Each accommodating hole 1201 of the accommodating plate 12 contains different types and concentrations of drugs, the sample flow channel 1202 is the only passage for the sample solution to enter the accommodating hole 1201 from the sample accommodating portion 114, the through hole 12015 at the lower end of the sample flow channel 1202 is sealed by the second sealing film 122, the first end 1204 is lower than the second end 1205, so that the first sealing film 121 can be tightly attached to the first end 1204, and the accommodating hole 1201 can be tightly sealed. The first sealing film 121 and the second sealing film 122 are matched to keep the accommodating hole 1201 completely sealed, and a negative pressure environment with a certain vacuum degree is formed in the accommodating hole 1201. The suction amount of the sample solution is controlled by controlling the degree of vacuum in the accommodation hole 1201, so that uniform and accurate transfer of the sample solution is more conveniently achieved.
The sample solution is poured into the sample addition groove 111, the sample solution flows into the main channel 113 through the liquid inlet 112, and is uniformly distributed to the sample accommodating part 114 through the main channel 113, the sample solution is at least higher than the through hole 12015 of the sample introduction channel 1202, and the redundant sample solution flows into the waste liquid groove 116 through the overflow port 115.
The containing plate 12 is arranged on the sample plate 11, in the installation process, the containing plate 12 presses the sample plate 11 downwards, the sealing ring 13 seals the sample plate 11 and the containing plate 12, and the sample solution in the sample plate 11 is prevented from overflowing through the contact surface. Meanwhile, the liquid guide needle 118 pierces the second sealing membrane 122, and under the action of the negative pressure in the accommodating hole 1201, the sample solution enters the accommodating hole 1201 along the liquid guide needle 118 through the sample injection channel 1202 and is mixed with the dry drug powder to form a mixed solution. The suction amount of the sample solution is controlled by controlling the degree of vacuum in the accommodation hole 1201, so that uniform and accurate transfer of the sample solution is more conveniently achieved.
Through control accommodation hole 1201 internal vacuum in this application, realize that sample solution from sample board 11 accurately transfer to accommodate board 12 in, saved the loaded down with trivial details process that traditional compoboard haplopore pipetted one by one, avoided changing the waste that disposable pipette tip caused simultaneously.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, variations and modifications can be made without departing from the spirit of the invention, and all equivalent modifications and changes can be made to the above embodiments according to the essential technology of the invention, which falls into the protection scope of the invention.
Claims (10)
1. A quick application of sample device which characterized in that: including the sample board and hold the board, the sample board is equipped with sample portion of acceping and a plurality of being located the sample portion of acceping liquid needle of acceping, it includes main part, first seal membrane and second seal membrane to hold the board, the main part is equipped with a plurality of accommodation holes and advances the kind runner, advance the kind runner with the accommodation hole intercommunication, first seal membrane is sealed the accommodation hole tip, the second seal membrane is sealed advance the kind runner, make the accommodation hole and with form the negative pressure in the kind runner of accommodation hole intercommunication, when quick application of sample device used, hold the board install in the sample board, it punctures to draw the liquid needle the second seal membrane makes the liquid sample in the sample portion of acceping follows advance the kind runner flows in each respectively the accommodation hole.
2. The rapid sample adding device according to claim 1, wherein: the number of the sample introduction flow passages is the same as that of the accommodating holes, each sample introduction flow passage extends from the bottom of the outer wall of each accommodating hole to a position close to the top, and the sample introduction flow passages are communicated with the insides of the accommodating holes at the positions close to the tops of the accommodating holes.
3. The rapid sample adding device according to claim 2, wherein: the inner wall of accommodation hole is equipped with protruding and sunken, protruding with sunken corresponding the setting and closing together form advance a kind runner, advance a kind runner's top with the accommodation hole intercommunication.
4. The rapid sample adding device according to claim 3, wherein: the accommodation hole is hollow structure, the accommodation hole includes the bottom surface, advance the kind runner and extend to the bottom surface and be in the bottom surface forms the through-hole, the second seal membrane is sealed the through-hole.
5. The rapid sample adding device according to claim 1, wherein: the liquid guide needle is of a hollow structure.
6. The rapid sample adding device according to claim 5, wherein: the liquid guide needle is provided with a notch, the notch extends along the extending direction of the liquid guide needle, and the interior of the liquid guide needle is communicated with the sample containing part through the notch.
7. The rapid sample adding device according to claim 5, wherein: the end part of the liquid guide needle is conical.
8. The rapid sample adding device according to claim 1, wherein: the sample plate is also provided with a sample adding groove, a liquid inlet, an overflow port and a waste liquid groove, wherein the sample adding groove is communicated with the sample accommodating part through the liquid inlet, and the sample accommodating part is communicated with the waste liquid groove through the overflow port.
9. The rapid sample adding device according to claim 8, wherein: the bottom of the sample adding slot is obliquely arranged, and the lowest part of the bottom of the sample adding slot is connected with the bottom of the liquid inlet.
10. The rapid sample adding device according to claim 1, wherein: the sample containing part is provided with a plurality of sample holes which are communicated with each other through a main runner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211699831.3A CN115945232A (en) | 2022-12-28 | 2022-12-28 | Quick application of sample device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211699831.3A CN115945232A (en) | 2022-12-28 | 2022-12-28 | Quick application of sample device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115945232A true CN115945232A (en) | 2023-04-11 |
Family
ID=87287426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211699831.3A Pending CN115945232A (en) | 2022-12-28 | 2022-12-28 | Quick application of sample device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115945232A (en) |
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2022
- 2022-12-28 CN CN202211699831.3A patent/CN115945232A/en active Pending
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