CN217809389U

CN217809389U - Immune cell in-vitro culture induction device

Info

Publication number: CN217809389U
Application number: CN202221918377.1U
Authority: CN
Inventors: 王仁杰
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-07-21
Filing date: 2022-07-21
Publication date: 2022-11-15
Anticipated expiration: 2032-07-21

Abstract

The utility model belongs to the technical field of the cell culture technique and specifically relates to an immune cell in vitro culture induction device, including the incubator, the inside of incubator is provided with culture mechanism, the humidifier is installed in the left side of the outer wall of incubator, gaseous holding vessel is installed on the right side of incubator, the honeycomb duct is all installed at the top of humidifier and gaseous holding vessel, the internally mounted of honeycomb duct has the solenoid valve, the chamber door is installed through the hinge in the front of incubator, control panel is just installed in the below of chamber door in the front of incubator, the display screen is installed in the front of chamber door, power plug is installed on the right side of incubator, through setting up incubator and culture mechanism, has solved current immune cell in vitro culture induction device when using, need open the device and come to add corresponding reagent to the immune cell in the cultivation, when adding, the device can't keep encapsulated situation, leads to the easy contaminated problem of immune cell.

Description

Immune cell in-vitro culture induction device

Technical Field

The utility model relates to a cell culture technical field specifically is an immune cell in vitro culture induction device.

Background

The device for inducing the in vitro culture of the immune cells is equipment for culturing the immune cells in vitro in a laboratory, but the existing device for inducing the in vitro culture of the immune cells still has the defects that: when the existing immune cell in-vitro culture induction device is used, the device needs to be opened to add corresponding reagents into immune cells in culture, and when the corresponding reagents are added, the device cannot keep a sealed state, so that the immune cells are easily polluted.

Therefore, there is a need for an apparatus for inducing in vitro culture of immune cells to solve the above-mentioned problems of the background art.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an immune cell in vitro culture induction device to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above purpose, the utility model provides a following technical scheme:

an immune cell in-vitro culture induction device comprises an incubator, wherein a culture mechanism is arranged in the incubator, a humidifier is installed on the left side of the outer wall of the incubator, a gas storage tank is installed on the right side of the incubator, guide pipes are installed at the tops of the humidifier and the gas storage tank, an electromagnetic valve is installed in the guide pipe, a box door is installed on the front of the incubator through a hinge, a control panel is installed on the front of the incubator and below the box door, a display screen is installed on the front of the box door, a power plug is installed on the right side of the incubator, an exhaust pipe is installed at the bottom of the inner wall of the incubator, a check valve is installed in the exhaust pipe, and a temperature and humidity sensor is installed at the top of the inner wall of the incubator;

cultivate the mechanism including installing the conveyer belt on incubator inner wall right side, the inside of incubator and have preceding flashboard in the top sliding connection of conveyer belt, the right side sliding connection of flashboard just in the front of the inside of incubator has the back flashboard, the equal plug-type electro-magnet of fixedly connected with in top of preceding flashboard and back flashboard, sealed the pad is all installed to the bottom of preceding flashboard and back flashboard, the sterilamp is just installed in the top of conveyer belt in the inside of incubator, the bottom of incubator inner wall just rotates on the right side of conveyer belt and is connected with the pivot, place the dish at the top of pivot, place the top of dish and seted up the standing groove, the surveillance camera head is installed at the top of incubator inner wall, electronic slide rail is just installed in the top of conveyer belt at the top of incubator, the slip table is installed to the outer wall of electronic slide rail, the bottom fixedly connected with electric putter of slip table, electric putter's bottom fixedly connected with connecting seat, the internally mounted of connecting seat has electronic pipette, the bottom fixedly connected with servo motor of pivot.

As the utility model discloses preferred scheme, incubator and chamber door are made by the aluminum alloy, the honeycomb duct runs through the incubator and extends to the inside of incubator, the storage has the high-pressure drying air through disinfection and sterilization processing in the gas storage tank.

As the utility model discloses preferred scheme, the check valve only allows the air outflow incubator, the observation window is installed in the front of chamber door and under the display screen, humidifier, solenoid valve, display screen, power plug, conveyer belt, plug-type electro-magnet, sterilamp, surveillance camera head, electric slide rail, electric putter, electronic pipette, temperature and humidity sensor and servo motor are electric connection with control panel's connected mode.

As the utility model discloses preferred scheme, preceding flashboard, back flashboard, place dish and connecting seat are made by the aluminum alloy, sterilamp, standing groove and surveillance camera head all are provided with the multiunit.

As the utility model discloses preferred scheme, the shape of standing groove and the shape looks adaptation of cell culture dish, sealed the pad is made by silica gel.

As the utility model discloses preferred scheme, the width of preceding flashboard and back flashboard and the width looks adaptation of conveyer belt, servo motor and incubator and the connected mode of plug-type electro-magnet and incubator is fixed connection, the connecting seat is L type structural design.

Compared with the prior art, the beneficial effects of the utility model are that:

1. in the utility model, through the arrangement of the incubator and the culture mechanism, when a worker uses the incubator and the culture mechanism to carry out in vitro induction culture on immune cells, the worker opens the box door, the culture dish inoculated with the immune cells is put on the placing plate, then the box door is closed, the control panel opens the electromagnetic valve in the guide pipe on the gas storage tank, high-pressure dry air is sterilized and enters the incubator, the original air in the incubator can be discharged through the exhaust pipe, when reagent is required to be added into the immune cells, the worker places the culture dish containing the reagent on the conveyor belt, the control panel starts the push-pull electromagnet on the front flashboard, the push-pull electromagnet can drive the front flashboard to move upwards, the conveyor belt drives the culture dish to move, the push-pull electromagnet can drive the front flashboard to return to the original position, the control panel starts the ultraviolet sterilizing lamp, the ultraviolet germicidal lamp can kill microorganisms in a culture dish and air, after sterilization treatment, the control panel starts a push-pull electromagnet on the flashboard, the push-pull electromagnet can drive the rear flashboard to move upwards, the culture dish is conveyed into the incubator by the conveyor belt, the control panel starts an electric slide rail, the electric slide rail drives an electric pipetting gun to move through a sliding table and enables the electric pipetting gun to aim at reagents in the culture dish, an electric push rod can push the electric pipetting gun to move downwards and insert the electric pipetting gun into the reagents, the control panel starts the electric pipetting gun which can suck the reagents with specified volumes, after the reagents are sucked, the electric push rod can drive the electric pipetting gun to move upwards, the electric pipetting gun withdraws from the reagents, meanwhile, the electric slide rail drives the electric pipetting gun to move rightwards through the sliding table and enables the electric pipetting gun to aim at immune cells, and the electric pipetting gun extrudes the sucked reagents, the reagent by extruding is dripped into immunocyte, at dropwise add reagent in-process, need not open the incubator, and the contaminated probability of immunocyte reduces, has solved current immunocyte in vitro culture induction system when using, need open the device and add corresponding reagent to the immunocyte in cultivateing, and when adding, the device can't keep a sealed state, leads to the easily contaminated problem of immunocyte.

Drawings

Fig. 1 is a front view of the present invention;

FIG. 2 is a front sectional view of the present invention;

fig. 3 is a schematic view of the three-dimensional structure of the rear gate plate of the present invention.

In the figure: 1. an incubator; 2. a culture mechanism; 3. a humidifier; 4. a gas storage tank; 5. a flow guide pipe; 6. an electromagnetic valve; 7. a box door; 8. a control panel; 9. a display screen; 10. a power plug; 11. an exhaust pipe; 12. an electromagnetic valve; 13. a temperature and humidity sensor; 201. a conveyor belt; 202. a front gate plate; 203. a rear gate plate; 204. a push-pull electromagnet; 205. a gasket; 206. an ultraviolet germicidal lamp; 207. a rotating shaft; 208. placing a tray; 209. a placement groove; 210. a surveillance camera; 211. an electric slide rail; 212. a sliding table; 213. an electric push rod; 214. a connecting seat; 215. an electric pipette; 216. a servo motor.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the scope of the present invention based on the embodiments of the present invention.

In order to facilitate understanding of the invention, the invention will be described more fully hereinafter with reference to the accompanying drawings, in which several embodiments of the invention are shown, but which can be embodied in many different forms and are not limited to the embodiments described herein, but rather are provided for the purpose of making the disclosure more thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present, that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present, and that the terms "vertical", "horizontal", "left", "right" and the like are used herein for descriptive purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in the specification of the present invention are for the purpose of describing particular embodiments only and are not intended to limit the present invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

In an embodiment, referring to fig. 1-3, the present invention provides a technical solution:

an immune cell in-vitro culture induction device comprises an incubator 1, wherein a culture mechanism 2 is arranged inside the incubator 1, a humidifier 3 is installed on the left side of the outer wall of the incubator 1, a gas storage tank 4 is installed on the right side of the incubator 1, a flow guide pipe 5 is installed on the tops of the humidifier 3 and the gas storage tank 4, an electromagnetic valve 6 is installed inside the flow guide pipe 5, a chamber door 7 is installed on the front side of the incubator 1 through a hinge, a control panel 8 is installed on the front side of the incubator 1 and below the chamber door 7, a display screen 9 is installed on the front side of the chamber door 7, a power plug 10 is installed on the right side of the incubator 1, an exhaust pipe 11 is installed at the bottom of the inner wall of the incubator 1, a one-way valve 12 is installed inside the exhaust pipe 11, and a temperature and humidity sensor 13 is installed on the top of the inner wall of the incubator 1;

wherein incubator 1 and chamber door 7 are made by the aluminum alloy, honeycomb duct 5 runs through incubator 1 and extends to the inside of incubator 1, the high pressure drying air through disinfection and sterilization is stored in gas holding vessel 4, the immune cell of cultivateing in the culture dish can not be polluted in the high pressure drying air through disinfection and sterilization gets into incubator 1, check valve 12 only allows the air to flow out incubator 1, the front of chamber door 7 just installs the observation window under the display screen 9, humidifier 3, solenoid valve 6, display screen 9, power plug 10, conveyer belt 201, plug-type electro-magnet 204, sterilamp 206, surveillance camera head 210, electronic slide rail 211, electric putter 213, electronic pipette 215, temperature and humidity sensor 13 and servo motor 216 are electric connection with the connected mode of control panel 8.

In this embodiment, referring to fig. 2 and 3, the culture mechanism 2 includes a conveyor belt 201 installed on the right side of the inner wall of the incubator 1, a front shutter 202 is slidably connected inside the incubator 1 above the conveyor belt 201, a rear shutter 203 is slidably connected inside the incubator 1 above the front shutter 202, push-pull electromagnets 204 are fixedly connected to the tops of the front shutter 202 and the rear shutter 203, gaskets 205 are installed at the bottoms of the front shutter 202 and the rear shutter 203, ultraviolet germicidal lamps 206 are installed inside the incubator 1 above the conveyor belt 201, a rotating shaft 207 is rotatably connected to the bottom of the inner wall of the incubator 1 and on the right side of the conveyor belt 201, a placing disc 208 is installed at the top of the rotating shaft 207, a placing groove 209 is opened at the top of the placing disc 208, a monitoring camera 210 is installed at the top of the inner wall of the incubator 1, an electric slide rail 211 is installed at the top of the incubator 1 above the conveyor belt 201, a sliding table 212 is installed at the outer wall of the electric slide rail 211, an electric push rod 213 is fixedly connected to the bottom of the sliding table 212, a connecting seat 214 is fixedly connected to the bottom of the electric push rod 213, a connecting seat 214 is fixedly connected to a servo motor 216;

wherein the front flashboard 202, the rear flashboard 203, the placing disc 208 and the connecting seat 214 are all made of aluminum alloy, the aluminum alloy material is light and durable, the service life is long, the manufacturing process is simple, the cost is low, the ultraviolet germicidal lamp 206, the placing groove 209 and the monitoring camera 210 are all provided with a plurality of groups, the shape of the placing groove 209 is matched with the shape of a cell culture dish, the sealing gasket 205 is made of silica gel, the widths of the front flashboard 202 and the rear flashboard 203 are matched with the width of the conveyer belt 201, the connection modes of the servo motor 216, the incubator 1 and the push-pull type electromagnet 204 and the incubator 1 are all fixedly connected, the connecting seat 214 is in an L-shaped structural design, when a worker uses the incubator 1 and the incubator 2 to carry out in-vitro induction culture on immune cells, the worker opens the door 7, puts the incubator inoculated with the immune cells into the placing disc 208, closes the door 7, and when a reagent is required to be added into the immune cells, A worker places a culture dish containing a reagent on a conveying belt 201, a control panel 8 starts a push-pull electromagnet 204 on a front flashboard 202, the push-pull electromagnet 204 can drive the front flashboard 202 to move upwards, the conveying belt 201 drives the culture dish to move, after the culture dish passes through the lower part of the front flashboard 202, the push-pull electromagnet 204 drives the front flashboard 202 to return to the original position, the control panel 8 starts an ultraviolet germicidal lamp 206, the ultraviolet germicidal lamp 206 can kill the culture dish and microorganisms in the air, after the sterilization treatment, the control panel 8 starts the push-pull electromagnet 204 on a rear flashboard 203, the push-pull electromagnet 204 can drive the rear flashboard 203 to move upwards, the conveying belt 201 sends the culture dish into a culture box 1, the control panel 8 starts an electric sliding rail 211, the electric sliding rail 211 drives an electric liquid-moving gun 215 to move through a sliding table 212, and the electric liquid-moving gun 215 is aligned with the reagent in the culture dish, electric putter 213 can promote electronic pipette 215 and move down, electronic pipette 215 inserts in the reagent, control panel 8 starts electronic pipette 215, electronic pipette 215 can inhale the reagent of appointed volume, the reagent absorbs the back, electric putter 213 can drive electronic pipette 215 and move up, electronic pipette 215 withdraws from the reagent, electronic slide rail 211 passes through slip table 212 and drives electronic pipette 215 and move right simultaneously, make electronic pipette 215 aim at the immune cell, electronic pipette 215 extrudes the reagent that absorbs, the reagent that is extruded can drip into the immune cell, in the dropwise add reagent process, need not open incubator 1, the contaminated probability of immune cell reduces.

The utility model discloses work flow: when a worker uses the incubator 1 and the culture mechanism 2 to perform in-vitro induction culture on immune cells, the worker opens the box door 7, places a culture dish inoculated with the immune cells into the placing tray 208, closes the box door 7, opens the electromagnetic valve 6 in the guide pipe 5 on the gas storage tank 4 by the control panel 8, high-pressure dry air enters the incubator 1 after disinfection and sterilization treatment, original air in the incubator 1 can be exhausted through the exhaust pipe 11, when a reagent needs to be added into the immune cells, the worker places the culture dish containing the reagent on the conveyer belt 201, the control panel 8 starts the push-pull electromagnet 204 on the front flashboard 202, the push-pull electromagnet 204 can drive the front flashboard 202 to move upwards, the conveyer belt 201 drives the culture dish to move, after the culture dish passes through the lower part of the front flashboard 202, the push-pull electromagnet 204 drives the front flashboard 202 to return to the original position, the control panel 8 starts the ultraviolet sterilizing lamp 206, the ultraviolet germicidal lamp 206 kills the culture dish and the microorganism in the air, after the germicidal treatment, the control panel 8 starts the push-pull electromagnet 204 on the back flashboard 203, the push-pull electromagnet 204 drives the back flashboard 203 to move upwards, the conveyor belt 201 feeds the culture dish into the incubator 1, the control panel 8 starts the electric slide rail 211, the electric slide rail 211 drives the electric pipetting gun 215 to move through the slide table 212, and makes the electric pipetting gun 215 aim at the reagent in the culture dish, the electric push rod 213 pushes the electric pipetting gun 215 to move downwards, the electric pipetting gun 215 is inserted into the reagent, the control panel 8 starts the electric pipetting gun 215, the electric pipetting gun 215 sucks the reagent with the designated volume, after the reagent is sucked, the electric push rod 213 drives the electric pipetting gun 215 to move upwards, the electric pipetting gun 215 withdraws from the reagent, and the electric slide rail 211 drives the electric pipetting gun 215 to move rightwards through the slide table 212, the electric pipette 215 is aligned to immune cells, the electric pipette 215 extrudes the absorbed reagent, the extruded reagent can be dripped into the immune cells, after the dripping process is finished, the control panel 8 starts the push-pull electromagnet 204 on the rear flashboard 203 first, then starts the push-pull electromagnet 204 on the front flashboard 202, the conveyer belt 201 runs reversely at the same time, so that a culture dish filled with the reagent is moved out of the incubator 1, the temperature and humidity sensor 13 can detect the temperature and humidity in the incubator 1, when the temperature is too high or the humidity is too high, the control panel 8 starts the electromagnetic valve 6 in the draft tube 5 on the gas storage tank 4, high-pressure dry air enters the incubator 1 after disinfection and sterilization treatment, the air with too high temperature or too high humidity in the incubator 1 can be discharged through the exhaust tube 11, when the humidity is too low, the control panel 8 starts the humidifier 3, the humidifier 3 sends the humid air into the incubator 1, the monitoring camera 210 can shoot the growth condition of the immune cells, and displays the shot picture on the display screen 9.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An induction device for in vitro culture of immune cells comprises an incubator (1), and is characterized in that: the incubator is characterized in that an incubation mechanism (2) is arranged inside the incubator (1), a humidifier (3) is installed on the left side of the outer wall of the incubator (1), a gas storage tank (4) is installed on the right side of the incubator (1), a flow guide pipe (5) is installed on the tops of the humidifier (3) and the gas storage tank (4), an electromagnetic valve (6) is installed inside the flow guide pipe (5), a chamber door (7) is installed on the front side of the incubator (1) through a hinge, a control panel (8) is installed on the front side of the incubator (1) and below the chamber door (7), a display screen (9) is installed on the front side of the chamber door (7), a power plug (10) is installed on the right side of the incubator (1), an exhaust pipe (11) is installed at the bottom of the inner wall of the incubator (1), a one-way valve (12) is installed inside the exhaust pipe (11), and a temperature and humidity sensor (13) is installed on the top of the inner wall of the incubator (1);

cultivate mechanism (2) including installing conveyer belt (201) on incubator (1) inner wall right side, inside of incubator (1) and in the top sliding connection of conveyer belt (201) preceding flashboard (202), right side sliding connection of inside and preceding flashboard (202) of incubator (1) has back flashboard (203), the equal fixedly connected with plug-type electro-magnet (204) in top of preceding flashboard (202) and back flashboard (203), sealed pad (205) are all installed to the bottom of preceding flashboard (202) and back flashboard (203), inside of incubator (1) and in the top of conveyer belt (201) install sterilamp (206), the bottom of incubator (1) inner wall just rotates on the right side of conveyer belt (201) and is connected with pivot (207), place dish (208) is installed at the top of pivot (207), place the top of dish (208) and seted up standing groove (209), surveillance head (210) are installed at the top of incubator (1) inner wall, the top of incubator (1) just installs in the top of conveyer belt (201) and install electric slide rail (211), electric slide rail (213) fixed slide rail (211), electric slide rail (213) bottom (213) is connected with electric slide rail (213), the inside of connecting seat (214) is installed with electronic pipetting gun (215), the bottom fixedly connected with servo motor (216) of pivot (207).

2. The device for inducing the in vitro culture of the immune cells as claimed in claim 1, wherein: incubator (1) and chamber door (7) are made by the aluminum alloy, honeycomb duct (5) run through incubator (1) and extend to the inside of incubator (1), the high pressure drying air through disinfection and sterilization processing is stored in gaseous holding vessel (4).

3. The device for inducing the in vitro culture of the immune cells according to claim 1, wherein: check valve (12) only allow air outflow incubator (1), the observation window is installed just under display screen (9) in the front of chamber door (7), humidifier (3), solenoid valve (6), display screen (9), power plug (10), conveyer belt (201), plug-type electro-magnet (204), sterilamp (206), surveillance camera head (210), electric slide rail (211), electric putter (213), electric pipetting gun (215), temperature and humidity sensor (13) and servo motor (216) and control panel (8)'s connected mode is electric connection.

4. The device for inducing the in vitro culture of the immune cells according to claim 1, wherein: the front flashboard (202), the rear flashboard (203), the placing disc (208) and the connecting seat (214) are all made of aluminum alloy, and the ultraviolet germicidal lamp (206), the placing groove (209) and the monitoring camera (210) are all provided with multiple groups.

5. The device for inducing the in vitro culture of the immune cells as claimed in claim 1, wherein: the shape of the placing groove (209) is matched with that of the cell culture dish, and the sealing gasket (205) is made of silica gel.

6. The device for inducing the in vitro culture of the immune cells according to claim 1, wherein: the width of preceding flashboard (202) and back flashboard (203) and the width looks adaptation of conveyer belt (201), servo motor (216) and incubator (1) and the connected mode of plug-type electro-magnet (204) and incubator (1) is fixed connection, connecting seat (214) are L type structural design.