CN208201002U - A kind of hypoxemia culture and detection integrated equipment - Google Patents
A kind of hypoxemia culture and detection integrated equipment Download PDFInfo
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- CN208201002U CN208201002U CN201820541998.XU CN201820541998U CN208201002U CN 208201002 U CN208201002 U CN 208201002U CN 201820541998 U CN201820541998 U CN 201820541998U CN 208201002 U CN208201002 U CN 208201002U
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- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 23
- 208000018875 hypoxemia Diseases 0.000 title abstract description 6
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 55
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- 238000000034 method Methods 0.000 description 5
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
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Abstract
The utility model provides a kind of hypoxemia culture and detection integrated equipment, including cultivating cabinet, detection box, temperature and humidity adjustment unit and control unit, the inside of culture cabinet is by separate layer at the culturing room of multiple sealings, the rear wall of culturing room is equipped with telescopic sealing door, bottom is equipped with pallet body, pallet body is towards being embedded with irony block on the side wall of hermatic door, the rear wall of detection box close to culture cabinet constitutes seal case, inside is equipped with lifting cylinder, lifting supporting plate, positioning mechanism and detection mirror, positioning mechanism includes positioning cylinder and the adsorption plate fixed with positioning cylinder piston rod, the surface of face hermatic door is embedded with electromagnet on adsorption plate, culturing room and the intracorporal wettability of detection case is adjusted in temperature and humidity adjustment unit, control unit and each component are electrically connected.The application can cultivate various kinds of cell sample simultaneously under different condition of culture, improve culture progress and efficiency, while can detect in detection box to the cell sample after culture.
Description
Technical Field
The utility model relates to the technical field of medical equipment, concretely relates to integrative equipment is cultivateed and detects to hypoxemia.
Background
The low-oxygen culture box is used in low-oxygen environment (O)2Concentration of<6%) of animal cells or anaerobic bacteria. The protein or enzyme expressed in vivo in anoxia or hypoxia environment is decomposed in the presence of oxygen, such as anoxia inducing factor-1 expressed at oxygen concentration of less than 6%, and anoxia inducing factor-1 is a transcription factor capable of inducing glycolysis gene expression and promoting anaerobic metabolism. Can promote angiogenesis by inducing the expression of vascular endothelial growth factor, and can also induce nitric oxide synthase, heme oxidase and expression to expand blood vessels. However, the inventor of the utility model finds through research that the existing hypoxia incubator has a simple structure, only a large culture chamber is arranged in the incubator body, only one type of cell sample can be cultured when the cell sample is cultured at each time, only queuing can be performed when multiple types of cell samples are required to be cultured simultaneously, and only one type of oxygen concentration can be set at each time, and the hypoxia experiment needs to test details in generalThe biological characteristics of cells under different oxygen concentrations, so that the traditional low-oxygen incubator greatly influences the progress and efficiency of culture; meanwhile, after the cultured cell sample is taken out of the incubator, the cell sample needs to be transferred to other equipment for sample detection, however, the biological characteristics of the cell sample are very easy to change in the transfer process, and therefore, the cell sample brings troubles to related work.
SUMMERY OF THE UTILITY MODEL
Aiming at the problems that the existing hypoxia incubator is simple in structure, only one large culture chamber is arranged in the incubator body, only one type of cell sample can be cultured each time when the cell sample is cultured, and only a queue can be waited when a plurality of types of cell samples need to be cultured simultaneously, so that the progress and the efficiency of the culture are seriously influenced; simultaneously, after the cell sample after cultivateing takes out from the incubator, need transfer to other equipment and carry out the sample detection, nevertheless the biological characteristic of cell sample changes very easily in the transportation, consequently has brought the technical problem of disturbance for relevant work, the utility model provides a novel hypoxia is cultivateed and is detected integrative equipment.
In order to solve the technical problem, the utility model discloses a following technical scheme:
a hypoxia culture and detection integrated device comprises a culture box body, a detection box body, a temperature and humidity adjusting unit and a control unit; wherein the interior of the culture box body divides the space in the box body into a plurality of sealed culture chambers by a laminate, the top of each culture chamber is provided with a first temperature sensor, a first humidity sensor, a light sensor and a first oxygen sensor, the left wall of each culture chamber is provided with a light source, the upper part of the right wall is provided with a first air inlet suitable for oxygen-deficient gas and oxygen to enter, a first air inlet pipe is correspondingly arranged on the first air inlet, a first electric heater is arranged in the middle of the right wall, a first air outlet is arranged at the lower part of the right wall, a first air outlet pipe is arranged on the first air outlet, a first adjusting valve is arranged on the first air inlet pipe and the first air outlet pipe, a closed chamber door is arranged on the front wall, a sealing door capable of being opened and closed in a telescopic mode is arranged on the rear wall, a tray mechanism suitable for placing culture utensils is arranged at the bottom of each culture chamber, and iron blocks are embedded into the side wall, facing the sealing door, of each tray mechanism; the rear wall of the detection box body close to the culture box body forms a sealed box body, a lifting cylinder, a lifting supporting plate, a positioning mechanism and a detection mirror are arranged inside the sealed box body, the lifting cylinder is fixed at the bottom of the detection box body, a piston rod of the lifting cylinder is vertically arranged, the lifting supporting plate is fixed at the top end of a piston rod of the lifting cylinder, the positioning mechanism comprises a positioning cylinder and an adsorption plate, the positioning cylinder is fixed on the surface of the lifting supporting plate, the piston rod of the positioning cylinder is horizontally arranged, the adsorption plate is fixed at the top end of the piston rod of the positioning cylinder and can be just adjacent to a sealing door, an electromagnet is embedded in the surface of the adsorption plate just opposite to the sealing door, a switch electrically connected with the electromagnet is arranged on the detection box body, the detection mirror is fixed at the top of the detection box body, and the rear wall of the, a second air inlet suitable for oxygen-deficient gas and oxygen to enter is formed in the upper portion of the right wall, a second air inlet pipe is correspondingly arranged on the second air inlet, a second electric heater is arranged in the middle of the right wall, a second air outlet is formed in the lower portion of the right wall, a second air outlet pipe is arranged on the second air outlet, and second adjusting valves are arranged on the second air inlet pipe and the second air outlet pipe; the integrated water storage tank is arranged below the culture box body and the detection box body, the temperature and humidity adjusting unit comprises a water suction pump, a water delivery pipe, an atomizing spray head and a switch valve, the water suction pump is arranged in the water storage tank, one end of the water delivery pipe is connected with a water discharge nozzle of the water suction pump, the other end of the water delivery pipe penetrates through the bottoms of the culture box body and the detection box body and is communicated with the atomizing spray head arranged at the top of each culture room and the left wall of the detection box body, a sealing ring is arranged between the water delivery pipe and the box body, and the switch valve is arranged on the water delivery pipe, close to the atomizing spray head, in each culture; the control unit is arranged on the front surface of the water storage tank, and the first temperature sensor, the second temperature sensor, the first humidity sensor, the second humidity sensor, the illumination sensor, the first oxygen sensor, the second oxygen sensor, the illumination light source, the first electric heater, the second electric heater, the lifting cylinder, the positioning cylinder, the switch valve and the water suction pump are all electrically connected with the control unit.
Compared with the prior art, the utility model provides an integrative equipment is cultivateed and detects to hypoxemia, separates into a plurality of sealed cultivations rooms with plywood from top to bottom with incasement space in the inside of cultivation box, constitutes a plurality of independent sealed cultivations rooms promptly inside cultivation box, from this when having multiple type cell sample to cultivate simultaneously, can put different types of cell sample into different sealed cultivations rooms and provide different culture conditions and cultivate, therefore has effectively promoted the progress and the efficiency of cultivation; the cell sample after the culture can be directly transferred into the detection box body for detection, and the specific process is as follows: firstly, the working condition inside the detection box body is adjusted to be the same as the culture condition of a culture chamber in which a cell sample to be detected is located, then the lifting cylinder drives the lifting supporting plate to move to a sealed door which is opposite to the culture chamber in which the cell sample to be detected is located, then the positioning cylinder drives the adsorption plate to pass through the sealed door, the tray mechanism is adsorbed on the adsorption plate through the adsorption effect of the electromagnet on an iron block after being electrified, then the positioning cylinder drives the adsorption plate and the tray mechanism to pass through the sealed door to return to the surface of the lifting supporting plate, the cells cultured in a culture vessel on the tray mechanism are detected through a detection mirror arranged at the top of the detection box body, after the detection is finished, the positioning cylinder drives the adsorption plate and the tray mechanism to pass through the sealed door to return to the culture chamber again, the electromagnet is powered off, the adsorption effect of the electromagnet on the iron block disappears, and, meanwhile, the sealing door is closed, so that the device has the functions of hypoxia culture and detection, and the biological characteristics of the cell sample cannot be changed in the detection and transportation process, thereby reducing the disturbance of related work; the control unit can carry out balance control to the detection condition that detects the box and the cultivation condition of every culture chamber, cultivates the box and directly sets up the water storage tank in the below of detecting the box, and accessible temperature and humidity control unit is direct for detecting the box and the inside humidity control of every culture chamber provides convenience.
Further, the illumination light source is an LED light source.
Further, a light guide plate is arranged at the light emitting end of the LED light source, a reflecting sheet is arranged behind the light guide plate, and a diffusion sheet is arranged in front of the light guide plate.
Further, be equipped with on the back wall of cultivateing the room and accept the chamber, the inside of accepting the chamber is equipped with telescopic cylinder and sealing door, telescopic cylinder's piston rod and sealing door fixed connection, telescopic cylinder and the control unit electric connection.
Furthermore, a contact sealing strip is arranged on the sealing door, and a soft sealing strip which can be meshed and sealed with the contact sealing strip is arranged on the sealing door frame on one side of the telescopic cylinder.
Further, tray mechanism includes bottom layer board and side shield, the bottom layer board is suitable for placing culture dish, side shield fixes the one end of bottom layer board and imbeds iron piece on the lateral wall towards sealing door.
Further, four corners of the bottom supporting plate are provided with rollers.
Furthermore, a drain valve is arranged on the wall of the water storage tank.
Drawings
FIG. 1 is a schematic structural view of the low-oxygen culture and detection integrated device provided by the present invention.
Fig. 2 is a schematic structural view of the detection box in fig. 1.
Fig. 3 is a schematic structural view of the tray mechanism in fig. 1.
In the figure, 1, a culture case; 10. laminating the board; 11. a culture chamber; 110. a first temperature sensor; 111. a first humidity sensor; 112. an illumination sensor; 113. a first oxygen sensor; 114. an illumination light source; 115. a first intake pipe; 116. a first electric heater; 117. a first air outlet pipe; 118. a secret room door; 119. a sealing door; 12. a tray mechanism; 121. a bottom pallet; 122. a side baffle; 123. a roller; 13. a ferrous mass; 2. detecting the box body; 21. a lifting cylinder; 22. lifting the supporting plate; 23. a positioning mechanism; 231. positioning the air cylinder; 232. an adsorption plate; 233. an electromagnet; 24. a detection mirror; 25. a switch; 261. a second temperature sensor; 262. a second humidity sensor; 263. a second oxygen sensor; 264. a second intake pipe; 265. a second electric heater; 266. a second air outlet pipe; 3. a temperature and humidity adjusting unit; 31. a water pump; 32. a water delivery pipe; 33. an atomizing spray head; 4. a control unit; 5. a water storage tank; 51. and (4) draining the water valve.
Detailed Description
In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand and understand, the present invention is further explained by combining with the specific drawings.
In the description of the present invention, it is to be understood that the terms "longitudinal", "radial", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and to simplify the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 to 3, the present invention provides an integrated apparatus for low oxygen culture and detection, which comprises a culture box 1, a detection box 2, a temperature and humidity adjusting unit 3 and a control unit 4; wherein, the inside of the culture box body 1 is divided into a plurality of sealed culture chambers 11 by a laminate 10, the top of each culture chamber 11 is provided with a first temperature sensor 110, a first humidity sensor 111, a light sensor 112 and a first oxygen sensor 113, the left wall of each culture chamber is provided with a light source 114, the upper part of the right wall is provided with a first air inlet suitable for oxygen-poor gas and oxygen to enter, the first air inlet is correspondingly provided with a first air inlet pipe 115, namely, the first air inlet pipe comprises a first oxygen-poor gas inlet pipe and a first oxygen inlet pipe, the first oxygen-poor gas inlet pipe can be directly communicated with an oxygen-poor gas bottle composed of nitrogen with the external volume percentage of 95% and carbon dioxide with the external volume percentage of 5%, and can be directly communicated with an external oxygen bottle by the first oxygen inlet pipe, thereby realizing accurate input of oxygen content, the middle part of the right wall is provided with a first electric heater 116 such as an electric heating wire, the lower part of the right wall is provided with a first air outlet, the first air outlet is provided with a first air outlet pipe 117, the first air inlet pipe 115 and the first air outlet pipe 117 are provided with first regulating valves, the front wall is provided with a sealing door 118, the rear wall is provided with a sealing door 119 capable of being opened and closed in a telescopic manner, the bottom of each culture chamber 11 is provided with a tray mechanism 12 suitable for placing culture utensils, the side wall of the tray mechanism 12 facing the sealing door 119 is embedded with an iron block 13, and different culture conditions can be provided for cells placed in the culture utensils on the tray mechanism 12 through the structural arrangement; the detection box body 2 is close to the back wall of the culture box body 1 to form a sealed box body, namely, the detection box body 2 shares the back wall of the culture box body 1, the detection box body 2 is internally provided with a lifting cylinder 21, a lifting supporting plate 22, a positioning mechanism 23 and a detection mirror 24, the lifting cylinder 21 is fixed at the bottom of the detection box body 2, a piston rod of the lifting cylinder 21 is vertically arranged, the lifting supporting plate 22 is fixed at the top end of the piston rod of the lifting cylinder 21, the positioning mechanism 23 comprises a positioning cylinder 231 and an adsorption plate 232, the positioning cylinder 231 is fixed on the surface of the lifting supporting plate 22, the piston rod of the positioning cylinder 231 is horizontally arranged, the adsorption plate 232 is fixed at the top end of the piston rod of the positioning cylinder and can be just abutted against the sealing door 119, the surface of the adsorption plate 232 just abutted against the sealing door 119 is embedded with an electromagnet 233, the detection box, the detection mirror 24 is fixed on the top of the detection box body 2, the detection mirror 24 can be an objective lens or a magnifier, etc., the rear wall of the detection box body 2 is provided with a second temperature sensor 261, a second humidity sensor 262 and a second oxygen sensor 263, the upper part of the right wall is provided with a second air inlet suitable for oxygen-deficient gas and oxygen to enter, the second air inlet is correspondingly provided with a second air inlet pipe 264, namely the second air inlet pipe comprises a second oxygen-deficient gas inlet pipe and a second oxygen inlet pipe, the second oxygen-deficient gas inlet pipe can be directly communicated with an oxygen-deficient gas bottle composed of 95% nitrogen and 5% carbon dioxide in external volume percentage, the second oxygen inlet pipe can be directly communicated with an external oxygen bottle, thereby realizing accurate input of oxygen content, the middle part of the right wall is provided with a second electric heater 265, and the lower part of the right wall is provided with a second air outlet, a second air outlet pipe 266 is arranged on the second air outlet, and a second regulating valve is arranged on the second air inlet pipe 264 and the second air outlet pipe 266; the culture box body 1 and the detection box body 2 are provided with an integrally formed water storage tank 5 below, the temperature and humidity adjusting unit 3 comprises a water suction pump 31, a water delivery pipe 32, an atomizing nozzle 33 and a switch valve, the water suction pump 31 is arranged in the water storage tank 5, one end of the water delivery pipe 32 is connected with a water discharge nozzle of the water suction pump 31, the other end of the water delivery pipe passes through the bottom parts of the culture box body 1 and the detection box body 2 and is communicated with an atomizing nozzle 33 arranged at the top part of each culture chamber and the left wall of the detection box body, namely, a water pipe 32 is transported and arranged in the detection box body 2 and each culture chamber 11, two ends of the water pipe 32 are respectively connected with a water suction pump 31 and an atomizing nozzle 33, and a sealing ring is arranged between the water delivery pipe 32 and the box body, the arrangement of the sealing ring can ensure the sealing independence of the detection box body 2 and each culture chamber 11, the switch valves are arranged on the water conveying pipes 32 which are arranged in each culture chamber 11 and the detection box body and are close to the atomizing nozzles 33; the control unit 4 is arranged on the front surface of the water storage tank 5, the first temperature sensor 110, the second temperature sensor 261, the first humidity sensor 111, the second humidity sensor 262, the illumination sensor 112, the first oxygen sensor 113, the second oxygen sensor 263, the illumination light source 114, the first electric heater 116, the second electric heater 265, the lifting cylinder 21, the positioning cylinder 231, the switch valve and the water pump 31 are all electrically connected with the control unit 4, the temperature sensors are used for detecting the temperature in the culture chamber and the detection tank body, the control unit compares the detected temperature, and when the detected temperature is less than the preset temperature, the electric heaters are started to heat the interior of the culture chamber and the detection tank body; the humidity sensor is used for detecting the humidity in the culture chamber and the detection box body, the control unit compares the detected humidity, and when the detected humidity is smaller than the preset humidity, the switch valve and the water pump are started to humidify and cool the interiors of the culture chamber and the detection box body; the illumination sensor is used for detecting the illumination intensity in the culture room, the control unit compares the detected illumination intensity, and when the detected illumination intensity is smaller than the preset illumination intensity, the control unit controls the illumination light source to brighten the interior of the culture room; the oxygen sensor is used for detecting the oxygen concentration in the culture chamber and the detection box body, the control unit compares the detected oxygen concentration, when the detected oxygen concentration is less than the preset oxygen concentration, the regulating valve on the air outlet pipe is opened to discharge the anoxic gas part, and the regulating valve on the oxygen inlet pipe is opened to input oxygen, so that the oxygen concentration in the culture chamber and the detection box body meets the requirement, and finally, the working condition in the detection box body and the culture condition in each culture chamber tend to be stable; the control unit simultaneously controls the start and stop of the lifting cylinder and the positioning cylinder; as an implementation mode, the control unit is controlled by a C51 series single chip microcomputer.
Compared with the prior art, the utility model provides an integrative equipment is cultivateed and detects to hypoxemia, separates into a plurality of sealed cultivations rooms with plywood from top to bottom with incasement space in the inside of cultivation box, constitutes a plurality of independent sealed cultivations rooms promptly inside cultivation box, from this when having multiple type cell sample to cultivate simultaneously, can put different types of cell sample into different sealed cultivations rooms and provide different culture conditions and cultivate, therefore has effectively promoted the progress and the efficiency of cultivation; the cell sample after the culture can be directly transferred into the detection box body for detection, and the specific process is as follows: firstly, the working condition inside the detection box body is adjusted to be the same as the culture condition of a culture chamber in which a cell sample to be detected is located, then the lifting cylinder drives the lifting supporting plate to move to a sealed door which is opposite to the culture chamber in which the cell sample to be detected is located, then the positioning cylinder drives the adsorption plate to pass through the sealed door, the tray mechanism is adsorbed on the adsorption plate through the adsorption effect of the electromagnet on an iron block after being electrified, then the positioning cylinder drives the adsorption plate and the tray mechanism to pass through the sealed door to return to the surface of the lifting supporting plate, the cells cultured in a culture vessel on the tray mechanism are detected through a detection mirror arranged at the top of the detection box body, after the detection is finished, the positioning cylinder drives the adsorption plate and the tray mechanism to pass through the sealed door to return to the culture chamber again, the electromagnet is powered off, the adsorption effect of the electromagnet on the iron block disappears, and, meanwhile, the sealing door is closed, so that the device has the functions of hypoxia culture and detection, and the biological characteristics of the cell sample cannot be changed in the detection and transportation process, thereby reducing the disturbance of related work; the control unit can carry out balance control to the detection condition that detects the box and the cultivation condition of every culture chamber, cultivates the box and directly sets up the water storage tank in the below of detecting the box, and accessible temperature and humidity control unit is direct for detecting the box and the inside humidity control of every culture chamber provides convenience.
As a specific embodiment, the illumination light source 114 is an LED light source, and the LED light source has high photoelectric conversion efficiency, and has the advantages of low dc voltage, long service life, low heat generation, small volume, and low energy consumption, so that the illumination light source can be used for short-distance illumination, and the space utilization rate in the culture chamber can be greatly improved.
In a specific embodiment, the light emitting end of the LED light source is provided with a light guide plate, that is, the light emitting end of the LED light source faces the light incident surface of the light guide plate, a reflective sheet is disposed behind the light guide plate, the reflective sheet is used for reflecting the light emitted from the back of the light guide plate into the light guide plate again, a diffusion sheet 14 is disposed in front of the light guide plate, and the diffusion sheet 14 is used for uniformly diffusing the light emitted from the front of the light guide plate, so that it is ensured that a light source with sufficient brightness and uniform distribution is obtained in each culture chamber, thereby facilitating the culture of cells.
As a specific embodiment, an accommodating cavity is formed in the rear wall of the cultivation chamber 11, that is, an interlayer accommodating cavity is formed in the rear wall of the cultivation chamber 11, a telescopic cylinder and a sealing door 119 are arranged inside the accommodating cavity, a piston rod of the telescopic cylinder is fixedly connected with the sealing door 119, and the telescopic cylinder is electrically connected with the control unit 4, so that the sealing door 119 can move left and right in the interlayer accommodating cavity under the driving of the piston rod of the telescopic cylinder, thereby realizing telescopic opening and closing.
As a specific embodiment, the sealing door 119 is provided with a contact sealing strip, and a sealing door frame on one side far away from the telescopic cylinder is provided with a soft sealing strip capable of being engaged and sealed with the contact sealing strip, so that the contact sealing strip on the sealing door 119 can be moved to be in extrusion fit with the soft sealing strip on the sealing door frame for sealing under the driving of a piston rod of the telescopic cylinder, and the culture chamber has higher and better air tightness.
In a specific embodiment, the tray mechanism 12 includes a bottom supporting plate 121 and a side baffle 122, the bottom supporting plate 121 is suitable for placing a culture vessel, the side baffle 122 is fixed at one end of the bottom supporting plate 121 and embedded with an iron block 13 on a side wall facing the sealing door 119, so that after the positioning cylinder 231 drives the adsorption plate 232 to pass through the sealing door 119, the iron block 13 on the side baffle 122 can be rapidly adsorbed with the electromagnet 233 on the adsorption plate 232.
As a specific example, the bottom of the bottom plate 121 is provided with rollers 123 at four corners, thereby facilitating the overall quick movement of the tray mechanism 12.
As a specific embodiment, a drain valve 51 is disposed on the wall of the water storage tank 5, thereby facilitating the drainage of water inside the water storage tank 5 and facilitating the water exchange.
As a specific embodiment, a sealing ring is arranged between the air inlet and the air inlet pipe, so that the culture chambers 11 and the detection box body 2 can be better sealed, and the gas content in the detection box body 2 and each culture chamber 11 is effectively ensured.
Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.
Claims (8)
1. A hypoxia culture and detection integrated device is characterized by comprising a culture box body, a detection box body, a temperature and humidity adjusting unit and a control unit; wherein,
the culture box body is internally divided into a plurality of sealed culture chambers by a laminate, the top of each culture chamber is provided with a first temperature sensor, a first humidity sensor, a light sensor and a first oxygen sensor, the left wall of each culture chamber is provided with a light source, the upper part of the right wall is provided with a first air inlet suitable for oxygen-deficient gas and oxygen to enter, a first air inlet pipe is correspondingly arranged on the first air inlet, a first electric heater is arranged in the middle of the right wall, a first air outlet is arranged at the lower part of the right wall, a first air outlet pipe is arranged on the first air outlet, a first adjusting valve is arranged on the first air inlet pipe and the first air outlet pipe, a closed chamber door is arranged on the front wall, a sealing door capable of being opened and closed in a telescopic mode is arranged on the rear wall, a tray mechanism suitable for placing culture utensils is arranged at the bottom of each culture chamber, and iron blocks are embedded into the side wall, facing the sealing door, of each tray mechanism;
the rear wall of the detection box body close to the culture box body forms a sealed box body, a lifting cylinder, a lifting supporting plate, a positioning mechanism and a detection mirror are arranged inside the sealed box body, the lifting cylinder is fixed at the bottom of the detection box body, a piston rod of the lifting cylinder is vertically arranged, the lifting supporting plate is fixed at the top end of a piston rod of the lifting cylinder, the positioning mechanism comprises a positioning cylinder and an adsorption plate, the positioning cylinder is fixed on the surface of the lifting supporting plate, the piston rod of the positioning cylinder is horizontally arranged, the adsorption plate is fixed at the top end of the piston rod of the positioning cylinder and can be just adjacent to a sealing door, an electromagnet is embedded in the surface of the adsorption plate just opposite to the sealing door, a switch electrically connected with the electromagnet is arranged on the detection box body, the detection mirror is fixed at the top of the detection box body, and the rear wall of the, a second air inlet suitable for oxygen-deficient gas and oxygen to enter is formed in the upper portion of the right wall, a second air inlet pipe is correspondingly arranged on the second air inlet, a second electric heater is arranged in the middle of the right wall, a second air outlet is formed in the lower portion of the right wall, a second air outlet pipe is arranged on the second air outlet, and second adjusting valves are arranged on the second air inlet pipe and the second air outlet pipe;
the integrated water storage tank is arranged below the culture box body and the detection box body, the temperature and humidity adjusting unit comprises a water suction pump, a water delivery pipe, an atomizing spray head and a switch valve, the water suction pump is arranged in the water storage tank, one end of the water delivery pipe is connected with a water discharge nozzle of the water suction pump, the other end of the water delivery pipe penetrates through the bottoms of the culture box body and the detection box body and is communicated with the atomizing spray head arranged at the top of each culture room and the left wall of the detection box body, a sealing ring is arranged between the water delivery pipe and the box body, and the switch valve is arranged on the water delivery pipe, close to the atomizing spray head, in each culture;
the control unit is arranged on the front surface of the water storage tank, and the first temperature sensor, the second temperature sensor, the first humidity sensor, the second humidity sensor, the illumination sensor, the first oxygen sensor, the second oxygen sensor, the illumination light source, the first electric heater, the second electric heater, the lifting cylinder, the positioning cylinder, the switch valve and the water suction pump are all electrically connected with the control unit.
2. The integrated hypoxic culture and detection apparatus according to claim 1, wherein the illumination light source is an LED light source.
3. The integrated low-oxygen culture and detection device as claimed in claim 2, wherein a light guide plate is disposed at the light emitting end of the LED light source, a reflective sheet is disposed at the back of the light guide plate, and a diffusion sheet is disposed at the front of the light guide plate.
4. The integrated low-oxygen culture and detection device according to claim 1, wherein a receiving cavity is formed in a rear wall of the culture chamber, a telescopic cylinder and a sealing door are arranged inside the receiving cavity, a piston rod of the telescopic cylinder is fixedly connected with the sealing door, and the telescopic cylinder is electrically connected with the control unit.
5. The hypoxia culture and detection integrated device according to claim 4, wherein a contact sealing strip is arranged on the sealing door, and a soft sealing strip which can be engaged and sealed with the contact sealing strip is arranged on a sealing door frame on the side far away from the telescopic cylinder.
6. The integrated hypoxic culture and detection device according to claim 1, wherein the tray mechanism comprises a bottom supporting plate and a side baffle, the bottom supporting plate is suitable for placing a culture vessel, and the side baffle is fixed at one end of the bottom supporting plate and embedded with an iron block on the side wall facing the sealing door.
7. The integrated hypoxic culture and detection device according to claim 6, wherein the bottom supporting plate is provided with rollers at four corners of the bottom.
8. The integrated hypoxic culture and detection device according to claim 1, wherein a drain valve is arranged on the wall of the water storage tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820541998.XU CN208201002U (en) | 2018-04-16 | 2018-04-16 | A kind of hypoxemia culture and detection integrated equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820541998.XU CN208201002U (en) | 2018-04-16 | 2018-04-16 | A kind of hypoxemia culture and detection integrated equipment |
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| Publication Number | Publication Date |
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| CN208201002U true CN208201002U (en) | 2018-12-07 |
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| CN201820541998.XU Expired - Fee Related CN208201002U (en) | 2018-04-16 | 2018-04-16 | A kind of hypoxemia culture and detection integrated equipment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108239601A (en) * | 2018-04-16 | 2018-07-03 | 重庆医科大学附属口腔医院 | A kind of hypoxemia culture and detection integrated system |
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2018
- 2018-04-16 CN CN201820541998.XU patent/CN208201002U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108239601A (en) * | 2018-04-16 | 2018-07-03 | 重庆医科大学附属口腔医院 | A kind of hypoxemia culture and detection integrated system |
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