CN217490917U - Micro-fluidic chip and in-vitro detection device - Google Patents

Abstract

The utility model relates to a micro-fluidic chip and external detection device, micro-fluidic chip include first body and reagent package. The first body is provided with a first cavity, the bottom wall of the first cavity is provided with a piercing part, and the side wall of the first cavity is provided with a first clamping part. When liquid in the reagent pack needs to be used, the reagent pack is pressed manually or by mechanical force, and when the pressing force reaches a preset value, the first clamping part and/or the second clamping part are/is deformed correspondingly under stress, so that the puncture piece can correspondingly puncture the sealing cover, and the liquid in the reagent pack flows out of the first cavity for use; when the liquid in the reagent pack is not used, for example, in a transportation state, the reagent pack is clamped and matched with the first clamping part through the second clamping part, so that the sealing cover can be contacted with the puncturing piece or a gap is arranged between the sealing cover and the puncturing piece, the sealing cover can be prevented from being easily punctured in the transportation process, and the shock resistance and the stability are improved.

Description

Micro-fluidic chip and in-vitro detection device

Technical Field

The utility model relates to a micro-fluidic technical field especially relates to a micro-fluidic chip and external detection device.

Background

In Vitro Diagnosis (IVD) refers to a process of taking a sample of blood, body fluid, tissue, etc. from a human body, and detecting and verifying the sample using an In Vitro detection reagent, an instrument, etc. In order to prevent, diagnose, detect treatment, observe later, evaluate health, predict genetic diseases, etc. the IVD is a method of diagnosing diseases In the body. The microfluidic chip technology (Microfluidics) can integrate basic operation units such as sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a chip, automatically complete the whole analysis process, greatly improve the detection efficiency, and simultaneously have the advantages of miniaturization, automation and the like, so the POCT field is more and more extensive.

The traditional microfluidic chip is provided with a reagent pack and a puncture structure. The reagent bag is provided with a silica gel film or a PE film which is easy to be punctured by the puncture structure at the position facing the puncture structure, the reagent bag is generally fixedly arranged through the friction force between the reagent bag and the position of the reagent bag, and when the reagent bag is subjected to external acting force, the reagent bag moves towards the puncture structure and is punctured by the puncture structure. However, during transportation, if vibration occurs, the puncture structure is likely to puncture the reagent pack, thereby causing the reagent in the reagent pack to fail.

SUMMERY OF THE UTILITY MODEL

Accordingly, it is necessary to overcome the defects of the prior art and provide a microfluidic chip and an in vitro detection device, which can improve the shock resistance and stability.

The technical scheme is as follows: a microfluidic chip, comprising: the first body is provided with a first cavity, the bottom wall of the first cavity is provided with a piercing part, and the side wall of the first cavity is provided with a first clamping part; the reagent bag is arranged in the first cavity and comprises a second body and a second clamping part which is arranged on the second body and is clamped and matched with the first clamping part, and when the first clamping part and the second clamping part are clamped with each other, the reagent bag is contacted with the piercing part or is provided with an interval; when the reagent pack is subjected to a driving force towards the piercing member, the reagent pack is movable towards the piercing member such that the piercing member pierces the reagent pack.

According to the micro-fluidic chip, when liquid in the reagent pack needs to be used, the reagent pack is pressed manually or mechanically, when the pressing force reaches a preset value, the first clamping portion and/or the second clamping portion deform correspondingly under stress, so that the puncture piece can puncture the reagent pack correspondingly, and the liquid in the reagent pack flows out of the first cavity for use; when liquid in the reagent package is not used, for example, when the microfluidic chip is in a transportation state, the reagent package is clamped and matched with the first clamping part through the second clamping part, so that the reagent package can be contacted with the puncture piece or a gap is formed between the reagent package and the puncture piece, the reagent package can be prevented from being easily punctured in the transportation process, and the shock resistance and the stability are improved.

In one embodiment, the first clamping part and the second clamping part are clamping blocks which are mutually abutted and matched, and at least one clamping block is an elastic block; or, first joint portion with one of them of second joint portion is the elasticity piece, another for with elasticity piece card is held the complex joint hole, the elasticity piece set up in it is downthehole to hold.

In one embodiment, the reagent pack is shaped to fit into the first chamber; the puncturing piece is positioned on the bottom wall of the first cavity and adjacent to the first clamping portion.

In one embodiment, the outer diameter of the piercing member increases from the head to the base thereof.

In one embodiment, the outer wall of the piercing member defines at least one flow channel extending from the head of the piercing member to the base of the piercing member.

In one embodiment, the reagent pack further comprises a sealing cover which is arranged on the second body and can be punctured by the puncturing piece; when the first clamping part and the second clamping part are clamped with each other, the sealing cover is contacted with the puncturing part or provided with an interval; when the reagent pack is subjected to a driving force towards the piercing member, the reagent pack is able to move towards the piercing member such that the piercing member pierces the sealing cap.

In one embodiment, the sealing cover is one or more of aluminum foil, PE film, silicone, rubber, plastic, and resin.

In one embodiment, the second body is provided with a liquid inlet and a boss circumferentially arranged around the liquid inlet, and the boss is positioned on the opening edge of the liquid inlet; the sealing cover is covered on the boss in a hot pressing mode.

In one embodiment, the height of the boss protruding from the edge of the liquid inlet is defined as h, and h is 0.01mm-2 mm; the distance between the inner wall surface and the outer wall surface of the boss is S, and S is 0.01mm-2 mm.

In one embodiment, the first body is further provided with a second chamber, a third chamber, a fourth chamber, a first channel, a second channel and a third channel; the first cavity is communicated with the second cavity through the first channel, the second cavity is communicated with the third cavity through the second channel, the second cavity is further communicated with the fourth cavity through the third channel, the distances from the second cavity, the third cavity and the fourth cavity to the rotation center of the first body are all larger than the distance from the first cavity to the rotation center, and the nearest distance from the third cavity to the rotation center is larger than the nearest distance from the fourth cavity to the rotation center.

In one embodiment, the first body is further provided with a fourth channel and an exhaust hole, one end of the fourth channel is communicated with the third chamber, and the other end of the fourth channel is communicated with the exhaust hole.

An in vitro detection device comprises the microfluidic chip.

According to the in-vitro detection device, when the liquid in the reagent pack needs to be used, the reagent pack is pressed manually or by mechanical force, and when the pressing force reaches a preset value, the first clamping part and/or the second clamping part deform correspondingly under stress, so that the puncture piece can puncture the reagent pack correspondingly, and the liquid in the reagent pack flows out of the first cavity for use; when the liquid in the reagent pack is not used, for example, in a transportation state, the reagent pack is clamped and matched with the first clamping part through the second clamping part, so that the reagent pack can be contacted with the puncturing piece or a gap is arranged between the reagent pack and the puncturing piece, the sealing cover can be prevented from being easily punctured in the transportation process, and the shock resistance and the stability are improved.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification.

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention;

FIG. 2 is an enlarged schematic view of FIG. 1 at A;

fig. 3 is an exploded view of a reagent pack according to an embodiment of the present invention;

FIG. 4 is a schematic structural view of a second body of the reagent pack shown in FIG. 3;

fig. 5 is a schematic view of one of the viewing angles of the puncturing member according to an embodiment of the present invention;

fig. 6 is a schematic view of another perspective structure of a puncturing member according to an embodiment of the present invention;

fig. 7 is a schematic view of a lancing member according to another embodiment of the present invention;

fig. 8 is a schematic view of another perspective structure of a puncturing member according to another embodiment of the present invention;

fig. 9 is a schematic view of a lancing device according to another embodiment of the present invention;

FIG. 10 is a schematic view of another embodiment of a piercing member of the present invention;

fig. 11 is a schematic view of a piercing member according to yet another embodiment of the present invention;

fig. 12 is a schematic view of another perspective structure of a puncturing member according to another embodiment of the present invention.

10. A first body; 11. a first chamber; 111. a piercing member; 1111. a flow channel; 112. a first clamping part; 12. a second chamber; 13. a third chamber; 14. a fourth chamber; 15. a first channel; 16. a second channel; 17. a third channel; 18. a fourth channel; 19. an exhaust hole; 20. a reagent pack; 21. a second body; 211. a liquid inlet; 212. a boss; 22. a sealing cover; 23. a second clamping part; 231. a bevel.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

Referring to fig. 1 to 3, fig. 1 shows a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention, fig. 2 shows an enlarged schematic structural diagram of fig. 1 at a, and fig. 3 shows an exploded schematic structural diagram of a reagent pack 20 according to an embodiment of the present invention. An embodiment of the present invention provides a micro-fluidic chip, which includes a first body 10 and a reagent pack 20. The first body 10 is provided with a first chamber 11, a piercing member 111 is arranged on the bottom wall of the first chamber 11, and a first clamping portion 112 is arranged on the side wall of the first chamber 11. The reagent pack 20 is disposed in the first chamber 11, and the reagent pack 20 includes a second body 21, and a second clamping portion 23 disposed on the second body 21 and clamped with the first clamping portion 112. When the first clamping portion 112 and the second clamping portion 23 are clamped to each other, the reagent pack 20 is in contact with or spaced from the piercing member 111. When the reagent pack 20 is subjected to a driving force towards the piercing member 111, the reagent pack 20 can move towards the piercing member 111 such that the piercing member 111 pierces the reagent pack 20.

Specifically, the reagent pack further comprises a sealing cover which is arranged on the second body and can be punctured by the puncturing piece. When the first clamping portion 112 and the second clamping portion 23 are clamped with each other, the sealing cover 22 is in contact with or spaced from the piercing member 111. When the reagent pack 20 is subjected to a driving force towards the piercing member 111, the reagent pack 20 can move towards the piercing member 111 such that the piercing member 111 pierces the sealing cap 22.

In the microfluidic chip, when the liquid in the reagent pack 20 needs to be used, the reagent pack 20 is pressed manually or by mechanical force, and when the pressing force reaches a preset value, the first clamping portion 112 and/or the second clamping portion 23 deform correspondingly under stress, so that the puncture piece 111 can correspondingly puncture the sealing cover 22, and the liquid in the reagent pack 20 flows out of the first chamber 11 for use; when the liquid in the reagent pack 20 is not used, for example, in a transportation state, the reagent pack 20 is engaged with the first engaging portion 112 via the second engaging portion 23, so that the sealing cover 22 contacts with or is spaced from the piercing member 111, the sealing cover 22 is prevented from being easily pierced during transportation, and the shock resistance and stability are improved.

Referring to fig. 1 to 3, in an embodiment, the first clamping portion 112 and the second clamping portion 23 are clamping blocks that are mutually abutted and matched, and at least one of the clamping blocks is an elastic block; alternatively, one of the first clamping portion 112 and the second clamping portion 23 is an elastic block, and the other is a clamping hole clamped and matched with the elastic block, and the elastic block is disposed in the clamping hole.

Specifically, in order to reduce the pressing force and facilitate the sealing cover 22 to be pierced by the piercing member 111 during pressing, the inclined surface 231 is provided at the contact portion between one of the holding blocks and the other of the holding blocks, and the two holding blocks will move along the inclined surface 231 during pressing, which is beneficial to pushing the reagent pack 20 toward the piercing member 111 so that the piercing member 111 pierces the sealing cover 22.

Referring to fig. 1-3, in one embodiment, the reagent pack 20 is shaped to fit into the first chamber 11. The piercing member 111 is located on the bottom wall of the first chamber 11 adjacent to the first catching portion 112. In this way, the piercing member 111 is close to the first clamping portion 112, so that the reagent pack 20 only needs to be spaced from or contacted with the piercing member 111 by matching the first clamping portion 112 and the second clamping portion 23 in a region corresponding to the piercing member 111, and other regions on the reagent pack 20 can be attached to the bottom wall of the first chamber 11, so that the reagent pack 20 can be stably installed in the first chamber 11, and the reagent pack 20 can have better shock resistance and stability; when the liquid in the reagent pack 20 needs to be used, the region provided adjacent to the puncture tool 111 may be pressed manually or mechanically, that is, the pressing force may be concentrated at one point, which facilitates the puncture of the reagent pack 20.

Referring to fig. 1 to 3, specifically, when the first chamber 11 is designed to be an arc segment, the piercing member 111 and the first clamping portion 112 are located at the same end of the first chamber 11, the piercing member 111 is located on the bottom wall of the end portion of the first chamber 11, and the first clamping portion 112 is located on the side wall of the end portion of the first chamber 11.

Referring to fig. 1 to 3, in an embodiment, the number of the puncturing elements 111 may be one, two, three, four or more, which is not limited herein, and can be flexibly set and adjusted according to actual requirements. As an example, there are two piercing members 111, and the two piercing members 111 are spaced apart and located at the same end of the bottom wall of the first chamber 11. Two piercing members 111 can form two piercing openings in the sealing cap 22.

Referring to fig. 5 to 12, fig. 5 to 12 respectively illustrate the structures of four different shapes of the puncturing element 111. In one embodiment, the outer diameter of the piercing member 111 increases from the head to the bottom of the piercing member 111, wherein the head of the piercing member 111 is the end of the piercing member 111 near the reagent pack 20, and the tail of the piercing member 111 is the end near the bottom wall of the first chamber 11. Thus, during the pressing process, the puncturing member 111 can facilitate puncturing the sealing cover 22, and the size of the opening formed on the sealing cover 22 gradually increases with the increasing degree of the pressing, so that the liquid in the reagent pack 20 can smoothly flow out after puncturing.

Referring to fig. 5-12, in one embodiment, the outer wall of the lancing device 111 is formed with at least one flow channel 1111 that extends from the head of the lancing device 111 to the bottom of the lancing device 111. Thus, by the design of the flow channel 1111, it is convenient to realize that when the puncture member 111 punctures the sealing cover 22, the liquid in the reagent bag 20 can timely flow out into the first chamber 11 through the flow channel 1111.

Referring to fig. 5 and 6, in one embodiment, the puncturing element 111 may be a cone, for example, and a flow channel 1111 may be formed on an outer wall of the cone, for example.

Referring to fig. 7-12, in one embodiment, the piercing member 111 may be a polygonal pyramid, including but not limited to a quadrangular pyramid as shown. Furthermore, for example one flow channel 1111 is provided on one of the side walls of the polygonal pyramid, or for example a flow channel 1111 is provided on at least two of the side walls.

In one embodiment, the piercing member 111 includes, but is not limited to, being formed on the bottom wall of the first chamber 11 by injection molding, 3D printing, or the like.

In one embodiment, the piercing member 111 is not limited to Polyethylene (PE), polypropylene (PP), Polycarbonate (PC), polyethylene terephthalate (PET), Polystyrene (PS), polymethyl methacrylate (PMMA), acrylonitrile butadiene polyethylene copolymer (ABS), metal, ceramic, etc.

In one embodiment, the sealing cover 22 is one or more of aluminum foil, PE film, silicone, rubber, plastic, and resin.

As one example, the sealing cover 22 is an aluminum foil. The aluminum foil is not easily pierced by the piercing member 111, and can be pierced by the piercing member 111 only when the pressing force is greater than a preset value, thereby improving the shock resistance.

Referring to fig. 3 and 4, in one embodiment, the second body 21 has a liquid inlet 211 and a boss 212 circumferentially disposed around the liquid inlet 211, and the boss 212 is located on the edge of the liquid inlet 211. The sealing cover 22 is covered on the boss 212 by a hot pressing method. So, because the circumference at inlet 211 encircles and is provided with boss 212, in-process on locating boss 212 with sealed lid 22 through the hot pressing mode lid, the whole round of boss 212 can both with sealed lid 22 in close contact with to sealed lid 22 lid is located behind the inlet 211, can guarantee that reagent package 20 has better leakproofness.

Referring to FIG. 3 and FIG. 4, in one embodiment, the height of the protrusion 212 from the inlet 211 is defined as h, which is 0.01mm-2 mm. The distance between the inner wall surface and the outer wall surface of the boss 212 is S, and S is 0.01mm-2 mm.

It should be noted that the "boss 212" may be a part of the second body 21, that is, the "boss 212" and the other part of the second body 21 are integrally formed; the "boss 212" may be manufactured separately and combined with the "other parts of the second body 21" as a whole.

Referring to fig. 1 and fig. 2, in an embodiment, the first body 10 is further provided with a second chamber 12, a third chamber 13, a fourth chamber 14, a first channel 15, a second channel 16 and a third channel 17. The first chamber 11 is communicated with the second chamber 12 through a first passage 15, the second chamber 12 is communicated with the third chamber 13 through a second passage 16, the second chamber 12 is also communicated with the fourth chamber 14 through a third passage 17, the distances from the second chamber 12, the third chamber 13 and the fourth chamber 14 to the rotation center (point O shown in fig. 1) of the first body 10 are all larger than the distance from the first chamber 11 to the rotation center, and the nearest distance from the third chamber 13 to the rotation center (the distance from point B to point O shown in fig. 1) is larger than the nearest distance from the fourth chamber 14 to the rotation center (the distance from point C to point O shown in fig. 1). Thus, after the liquid in the reagent pack 20 flows out of the first chamber 11, under the action of centrifugal force, the liquid in the first chamber 11 enters the second chamber 12 through the first channel 15, and because the closest distance between the third chamber 13 and the rotation center is greater than the closest distance between the fourth chamber 14 and the rotation center, the liquid in the second chamber 12 enters the third chamber 13 through the second channel 16, a preset amount of liquid is filled in the third chamber 13, and then the liquid is discharged into the fourth chamber 14 through the third channel 17, and redundant liquid is collected through the fourth chamber 14. In this way, the combination of the second chamber 12, the third chamber 13 and the fourth chamber 14 can quantify the liquid volume to obtain quantitative liquid in the third chamber 13.

It is understood that the liquid in the reagent pack 20 includes, but is not limited to, a dilution liquid, and may be other liquids, and is flexibly set and adjusted according to actual requirements.

Referring to fig. 1 and 2, in one embodiment, the first body 10 further includes a fourth channel 18 and an exhaust hole 19. One end of the fourth passage 18 communicates with the third chamber 13, and the other end of the fourth passage 18 communicates with the exhaust hole 19. In this way, after the liquid in the second chamber 12 enters the third chamber 13 through the second passage 16, the gas in the third chamber 13 will be exhausted through the fourth passage 18 and the exhaust hole 19.

Specifically, the third chamber 13 is an arc segment, one end of the third chamber 13 is communicated with the second passage 16, and the other end of the third chamber 13 is communicated with the fourth passage 18.

Referring to fig. 1 to 3, in one embodiment, an in vitro testing device includes the microfluidic chip according to any one of the embodiments.

In the in-vitro detection device, when the liquid in the reagent pack 20 needs to be used, the reagent pack 20 is pressed manually or mechanically, and when the pressing force reaches a preset value, the first clamping portion 112 and/or the second clamping portion 23 are deformed correspondingly under stress, so that the puncture piece 111 can correspondingly puncture the sealing cover 22, and the liquid in the reagent pack 20 flows out of the first chamber 11 for use; when the liquid in the reagent pack 20 is not used, for example, in a transportation state, the reagent pack 20 is engaged with the first engaging portion 112 via the second engaging portion 23, so that the sealing cover 22 is in contact with or spaced from the piercing member 111, thereby preventing the sealing cover 22 from being easily pierced during transportation, and improving shock resistance and stability.

All possible combinations of the technical features in the above embodiments may not be described for the sake of brevity, but should be considered as being within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.

The above embodiments only represent several embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be interconnected within two elements or in a relationship where two elements interact with each other unless otherwise specifically limited. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, a first feature "on" or "under" a second feature may be directly contacting the second feature or the first and second features may be indirectly contacting the second feature through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature "under," "beneath," and "under" a second feature may be directly under or obliquely under the second feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Claims (12)

1. A microfluidic chip, comprising:

the first body is provided with a first cavity, the bottom wall of the first cavity is provided with a piercing piece, and the side wall of the first cavity is provided with a first clamping part; and

the reagent bag is arranged in the first cavity and comprises a second body and a second clamping part which is arranged on the second body and is clamped and matched with the first clamping part, and when the first clamping part and the second clamping part are clamped with each other, the reagent bag is in contact with the puncture piece or is provided with an interval; when the reagent pack is subjected to a driving force towards the piercing member, the reagent pack is movable towards the piercing member such that the piercing member pierces the reagent pack.

2. The microfluidic chip according to claim 1, wherein the first clamping portion and the second clamping portion are clamping blocks which are mutually abutted and matched, and at least one of the clamping blocks is an elastic block; or, first joint portion with one of them of second joint portion is the elasticity piece, another for with elasticity piece card is held the complex joint hole, the elasticity piece set up in it is downthehole to hold.

3. The microfluidic chip according to claim 1, wherein the reagent pack is shaped to fit into the first chamber; the puncturing piece is positioned on the bottom wall of the first cavity and adjacent to the first clamping portion.

4. The microfluidic chip according to claim 1, wherein the outer diameter of the piercing member increases in a direction from the head to the bottom thereof.

5. The microfluidic chip according to claim 4, wherein the outer wall of the piercing member has at least one flow channel formed thereon, the flow channel extending from the head of the piercing member to the bottom of the piercing member.

6. The microfluidic chip according to claim 1, wherein the reagent pack further comprises a sealing cap disposed on the second body and capable of being punctured by the puncturing member; when the first clamping part and the second clamping part are clamped with each other, the sealing cover is contacted with the puncturing part or provided with an interval; when the reagent pack is subjected to a driving force towards the piercing member, the reagent pack is able to move towards the piercing member such that the piercing member pierces the sealing cap.

7. The microfluidic chip according to claim 6, wherein the sealing cap is one or more of aluminum foil, PE film, silica gel, rubber, plastic, and resin.

8. The microfluidic chip according to claim 1, wherein the second body is provided with a liquid inlet, and a boss circumferentially arranged around the liquid inlet, the boss being located on an edge of the liquid inlet; the sealing cover is covered on the boss in a hot pressing mode.

9. The microfluidic chip according to claim 8, wherein the height of the protrusion of the boss from the edge of the inlet is defined as h, and h is 0.01mm to 2 mm; the distance between the inner wall surface and the outer wall surface of the boss is S, and S is 0.01mm-2 mm.

10. The microfluidic chip according to claim 1, wherein the first body further comprises a second chamber, a third chamber, a fourth chamber, a first channel, a second channel and a third channel; the first cavity is communicated with the second cavity through the first channel, the second cavity is communicated with the third cavity through the second channel, the second cavity is further communicated with the fourth cavity through the third channel, the distances from the second cavity, the third cavity and the fourth cavity to the rotation center of the first body are all larger than the distance from the first cavity to the rotation center, and the nearest distance from the third cavity to the rotation center is larger than the nearest distance from the fourth cavity to the rotation center.

11. The microfluidic chip according to claim 10, wherein the first body further comprises a fourth channel and a vent hole, one end of the fourth channel is connected to the third chamber, and the other end of the fourth channel is connected to the vent hole.

12. An in vitro test device, comprising the microfluidic chip according to any one of claims 1 to 11.

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