CN217459403U

CN217459403U - A mixing device for cell is broken up

Info

Publication number: CN217459403U
Application number: CN202221162242.7U
Authority: CN
Inventors: 舒凯; 谷佳; 刘青
Original assignee: Jiangsu Ruiyuan Biotechnology Co ltd
Current assignee: Jiangsu Ruiyuan Biotechnology Co ltd
Priority date: 2022-05-13
Filing date: 2022-05-13
Publication date: 2022-09-20
Anticipated expiration: 2032-05-13

Abstract

The utility model discloses a mixing device for scattering cells, which belongs to the technical field of adherent cell mixing, and comprises a base, an air bag and a mixing vessel, wherein the mixing vessel is arranged on the base, a support rod vertical to the base is arranged at one end of the base, a mounting seat is arranged on the support rod, a cylindrical cavity is arranged at one end of the mounting seat far away from the support rod, the air bag is arranged in the cylindrical cavity, and a pressing structure for driving the air bag to move is arranged in the mounting seat; the pressing structure comprises a support, a cam and a sliding rod, wherein the cam which is rotatably connected with the support is arranged at one end of the support, which is far away from the cylindrical cavity, the first sliding groove which is parallel to the support is arranged at the other end of the support, and the sliding rod is slidably connected with the support through the first sliding groove; the first sliding chute is arranged on the axis of the cylindrical cavity; the one end that the mount pad was kept away from in the cylinder chamber is equipped with the connecting pipe, is equipped with the tympanites pipe that one end gos deep into the mixing ware on the connecting pipe, and this device is applicable in adherence cell suspension mixing, and the mixing process goes on automatically.

Description

A mixing device for cell is broken up

Technical Field

The utility model relates to an adherence cell mixing technical field specifically is a mixing device that is used for the cell to break up.

Background

Most of the existing cell mixing devices in the market are manufactured on the basis of a stirring paddle or magnetic beads, are applied to mixing and subpackaging of suspended cells, are not suitable for mixing adherent cell suspensions, and generally can cause that the cells cannot be mixed uniformly or are damaged. For the uniform mixing of the adherent cell suspension, an artificial uniform mixing method is generally adopted. In the case of mesenchymal stem cells, the mesenchymal stem cells are precipitated at the bottom of the container in a short time by the influence of gravity in a static state. Because the mesenchymal stem cells are living cells, the uniform mixing mode is required to blow the cells uniformly and disperse the cells in the cell suspension, and the living cells cannot be damaged too severely. The existing mixing mode mainly carries out mixing by reversing mixing and carrying out blowing and sucking operations on cell suspension by using an electric suction assisting device and a pipette, so that limitation exists on large-volume cell suspension mixing, and uneven cell suspension mixing is easily caused, thereby affecting the quality control of products. In the prior art, the cell suspension is uniformly mixed firstly and then sampled and subpackaged, and the process is circulated until the cell stock solution is subpackaged, so that the efficiency is not high.

SUMMERY OF THE UTILITY MODEL

The technique that exists is not enough to the aforesaid, the utility model aims at providing a mixing device for cell is broken up to solve the problem of among the prior art cell suspension mixing inefficiency.

In order to solve the technical problem, the utility model adopts the following technical scheme: the utility model provides a mixing device for cell scattering, which is characterized in that the mixing device comprises a base, an air bag and a mixing vessel, wherein the mixing vessel is arranged on the base, a supporting rod vertical to the base is arranged at one end of the base, a mounting seat is arranged on the supporting rod, a cylindrical cavity is arranged at one end of the mounting seat far away from the supporting rod, the air bag is arranged in the cylindrical cavity, and a pressing structure for driving the air bag to move is arranged in the mounting seat; the pressing structure comprises a first support, a cam and a sliding rod, wherein the cam which is rotatably connected with the first support is arranged at one end, away from the cylindrical cavity, of the first support, the first sliding groove which is parallel to the first support is arranged at the other end of the first support, and the sliding rod is slidably connected with the first support through the first sliding groove; the first sliding chute is arranged on the axis of the cylindrical cavity; the one end that the mount pad was kept away from to the cylinder chamber is equipped with the connecting pipe, is equipped with the tympany pipe that one end gos deep into the mixing ware on the connecting pipe.

Preferably, a second sliding groove is formed in the base, and the blending vessel is slidably connected with the base through the second sliding groove.

Preferably, the support rod is further provided with a support frame slidably connected with the support rod, and one end of the support frame, which is far away from the support rod, is provided with a cavity matched with the tympanum pipe.

Preferably, one end of the cylindrical cavity, which is close to the mounting seat, is uniformly provided with a plurality of clamping blocks, the mounting seat is provided with clamping grooves matched with the clamping blocks, and the cylindrical cavity is detachably connected with the mounting seat through the clamping blocks and the clamping grooves.

Preferably, one end of the cylindrical cavity, which is far away from the mounting seat, is provided with a ring body sleeved outside the connecting pipe and the cylindrical cavity, and the ring body is rotatably connected with the cylindrical cavity through threads.

Preferably, the side surface of the support frame is provided with a first fastening screw matched with the support rod.

Preferably, one end of the sliding rod, which is close to the cylindrical cavity, is further provided with a pressing plate.

Preferably, the bottom of the blending dish is conical.

Preferably, the blending vessel is provided with a cover plate, and the cover plate is provided with a sampling port.

Preferably, one end of the mounting seat close to the supporting rod is slidably connected with the supporting rod, and a second fastening screw matched with the supporting rod is arranged on the mounting seat.

Preferably, be equipped with sampling device on the mixing ware side, sampling device includes sampling tube, second support and vacuum pump, second support one end is equipped with the tee bend lid, and the other end is through the third spout of locating the mixing ware side and mixing ware slidable connection, the tee bend is covered and is equipped with sample inlet pipe and the exhaust tube that runs through the tee bend lid to and can dismantle the sampling tube of being connected with the tee bend lid, sample inlet pipe both ends are connected with mixing ware, sampling tube respectively, the exhaust tube both ends are connected with vacuum pump, sampling tube respectively, the tee bend is covered and still is equipped with the vacuum bleed valve, be equipped with the mounting that passes third spout lateral wall and second support butt on the third spout.

The beneficial effects of the utility model reside in that: 1. the utility model has simple integral structure and convenient operation, can be suitable for mixing large-volume cell suspension and adherent cell suspension, automatically performs the mixing process, reduces manual operation and labor amount; 2. the mixing and sampling can be carried out synchronously, the mixing does not influence the sampling, and the efficiency is improved; 3. the device is convenient to mount, dismount, sterilize and clean, and ensures the subsequent use of the cell blending liquid; 4. the device is suitable for mixing large-volume (more than 1000ml) cell suspension.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a blending device for cell scattering according to an embodiment of the present invention.

Fig. 2 is a side view of a mixing device for cell scattering according to an embodiment of the present invention.

Fig. 3 is a side sectional view of a mixing device for cell scattering according to an embodiment of the present invention.

Fig. 4 is a front cross-sectional view of a blending device for cell scattering according to an embodiment of the present invention.

Fig. 5 is a schematic diagram of a cylindrical cavity structure of a mixing device for cell scattering according to an embodiment of the present invention.

Fig. 6 is a schematic structural diagram of a blending device for cell scattering according to another embodiment of the present invention.

Fig. 7 is a schematic structural view of a sampling device of a mixing device for cell scattering according to an embodiment of the present invention.

Description of the reference numerals: a base 1; an air bag 2; a support rod 3; a mounting seat 4; a cylindrical cavity 5; a first bracket 6; a cam 7; a slide bar 8; a first chute 9; a connecting pipe 10; an air blast tube 11; a blending dish 12; a second chute 13; a support frame 14; a shaped cavity 15; a clamping block 16; a ring body 17; a first fastening screw 18; a pressing plate 19; a cover plate 20; a sampling port 21; a card slot 22; a second fastening screw 23; a runner 24; a second bracket 25; a three-way cover 26; a third chute 27; a fixing member 28; a sample introduction tube 29; an exhaust tube 30; a vacuum pump 31; a vacuum purge valve 32; a sampling tube 33.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The embodiment is as follows: as shown in fig. 1 to 5, the utility model provides a mixing device for scattering cells, including base 1, gasbag 2 and mixing ware 12, gasbag 2 is to the air-blowing in mixing ware 12, reach the purpose that realizes the cell scattering mixing, be equipped with mixing ware 12 on the base 1, base 1 one end is equipped with the bracing piece 3 perpendicular with base 1, be equipped with mount pad 4 on the bracing piece 3, the one end that the bracing piece 3 was kept away from to mount pad 4 is equipped with cylinder chamber 5, cylinder chamber 5 is located on the outside lateral wall of mount pad 4, and cylinder chamber 5 inside communicates with mount pad 4 inside, gasbag 2 locates inside cylinder chamber 5, mount pad 4 inside is equipped with the press structure that drives gasbag 2 motion; the pressing structure comprises a first support 6, a cam 7 and a sliding rod 8, the first support 6 is cross-shaped, the first support 6 is vertically arranged inside the mounting seat 4, two ends of a transverse branch of the first support 6 are respectively fixed with the side wall of the mounting seat 4, so that the first support 6 and the mounting seat 4 do not slide relatively, one end, away from the cylindrical cavity 5, of the vertical branch of the first support 6 is provided with the cam 7 which is rotatably connected with the first support 6, the other end of the vertical branch of the first support 6 is provided with a first sliding chute 9 which is parallel to the first support 6, the sliding rod 8 is slidably connected with the first support 6 through the first sliding chute 9, the cam 7 is connected with an external motor to drive the sliding rod 8 to slide, and the first sliding chute 9 is arranged on the axis of the cylindrical cavity 5; the one end that mount pad 4 was kept away from to cylinder chamber 5 is equipped with connecting pipe 10, is equipped with one end on the connecting pipe 10 and gos deep into the inside tympany tube 11 of mixing dish 12, and gasbag 2 is through connecting

pipe

10 and 11 intercommunications of tympany tube, and gasbag 2 is through connecting

pipe

10 and 11 tympany tubes to mixing dish in the drum gas, the concrete performance does: when the cam 7 rotates, the cam 7 drives the slide bar 8 to slide along the first sliding groove 9, the slide bar is close to or far away from the air bag 2, when the slide bar 8 is close to and extrudes the air bag 2, the air bag 2 generates elastic deformation, the air in the air bag 2 is blown out, the air enters the mixing vessel 12 through the connecting pipe 10 and the air blowing pipe 11, the cell is driven to move, when the cam 7 continues to rotate, the slide bar 8 loses the driving force of the cam 7, the air bag 2 is restored to the original state due to self elasticity, because the cell liquid forms a liquid seal, the pressure difference is formed inside and outside the air blowing pipe 11, the cell liquid enters the air blowing pipe 11 through the pressure difference, therefore, the cam 7 continuously rotates, the cell liquid is driven to continuously enter and exit the air blowing pipe 11, the process of artificially mixing the cell liquid is simulated, and the mixing of the cell liquid is realized.

Further, be equipped with second spout 13 on the base 1, mixing dish 12 passes through second spout 13 and base 1 slidable, can dismantle the connection, mixing dish 12 slides on base 1, adjust mixing dish 12 and the relative position of tympanic pipe 11, the cell sap mixing of being convenient for, base 1 preferably adopts PEI (polyetherimide) material, the VHP sterilization of being convenient for use, repeatedly usable, mixing dish 12 can dismantle with base 1 and be connected, be convenient for adjust the size of mixing dish 12, and then be applicable to the cell suspension mixing that the volume is big.

Furthermore, be equipped with on the bracing piece 3 with support meal 3 sliding connection's support frame 14, the one end that the bracing piece 3 was kept away from to support frame 14 is equipped with the chamber 15 (not drawn in the figure) with the cooperation of tympanites pipe 11, and tympanites pipe 11 joint is inside the chamber 15 that holds to fixed tympanites pipe 11, the chamber 15 can be changed into clamping structure, realize that the fixed of tympanites pipe 11 can.

Furthermore, a plurality of clamping blocks 16 are uniformly arranged at one end of the cylindrical cavity 5 close to the mounting seat 4, a clamping groove 22 matched with the clamping blocks 16 is arranged on the mounting seat 4, the cylindrical cavity 5 is detachably connected with the mounting seat 4 through the clamping blocks 16 and the clamping groove 22, and due to the particularity of cell liquid and the cleaning requirement of the mixing device, the cylindrical cavity 5 and the mounting seat 4 are detachably connected, so that whether the air bag 2 is damaged or not can be conveniently checked in the using process, the air bag 2 can be conveniently replaced, and meanwhile, the mixing device can be conveniently cleaned and sterilized, when in actual use, the clamping blocks 16 of the cylindrical cavity 5 are aligned to the clamping grooves 22 (not shown in the figure) of the mounting seat 4, the cylindrical cavity 5 is firstly pushed to enable the end surface of the cylindrical cavity 5 to be in contact with the outer surface of the mounting seat 4, then the mounting seat 4 is rotated to enable the clamping blocks 16 to be mutually staggered with the clamping grooves 22, thereby realizing the fixation of the cylindrical cavity 5 and the mounting seat 4, the cylinder cavity 5 can be disassembled by rotating the cylinder cavity 5 reversely.

Further, the one end that mount pad 4 was kept away from to cylinder chamber 5 is equipped with the cover and is equipped with ring 17 in connecting pipe and the outside of cylinder chamber 5, and ring 17 passes through screw thread rotatable coupling with cylinder chamber 5, and the ring 17 cover is established in the outside of cylinder chamber 5 and connecting pipe 10, rotates ring 17, and ring 17 and cylinder chamber 5 relative slip extrude the one end that blast pipe 11 and connecting pipe 10 are connected, the dismantlement, clearance, the sterilization of blast pipe 11 of being convenient for.

Furthermore, a first fastening screw 18 matched with the support rod 3 is arranged on the side surface of the support frame 14, and the support frame 14 is fixed on the support rod 3 through the first fastening screw 18.

Furthermore, one end of the sliding rod 8 close to the cylindrical cavity 5 is provided with a pressing plate 19, and the pressing plate 19 enables the sliding rod 8 to press the air bag 2, so that the stress of the air bag 2 is more uniform.

Furthermore, the bottom of the mixing dish 12 is conical, which is convenient for mixing the adherent cells and the cells sinking to the bottom.

Further, a cover plate 20 is arranged on the blending vessel 12, and a sampling port 21 is arranged on the cover plate 20.

Furthermore, but mount pad 4 is close to the 3 sliding connection of one end bracing piece of bracing piece 3, be equipped with on mount pad 4 with bracing piece 3 complex second fastening screw 23, mount pad 4 slides from top to bottom along bracing piece 3, adjusts the relative distance of mount pad 4 and mixing ware 12, and second fastening screw 23 makes mount pad 4 fix on bracing piece 3.

Further, the cam 7 is connected with an external motor to provide driving force for the movement of the air bag 2.

Furthermore, one end of the slide bar 8 close to the cam 7 is provided with a rotating wheel 24, and the rotating wheel 24 reduces the friction between the slide bar 8 and the cam 7.

Further, a temperature control module may be disposed in the mixing dish 12 to keep the cell fluid at a constant temperature. Example 2:

on the basis of embodiment 1, for convenient follow-up ration is sample regularly, the utility model discloses be equipped with sampling device on mixing ware 12 side, sampling device includes sampling tube 33, second support 25 and vacuum pump 31, second support 25 one end is equipped with tee bend lid 26, the other end is through locating third spout 27 and mixing ware 12 slidable connection of mixing ware 12 side, third spout 27 is located on the outside lateral wall of mixing ware 12, be equipped with sample tube 29 and exhaust tube 30 that run through tee bend lid 26 on the tee bend lid 26, and can dismantle the sampling tube 33 of being connected with tee bend lid 26, in other words, sample tube 29 is connected respectively to three connecting holes on the tee bend lid 26, sampling tube 30, tee bend lid 26 and sampling tube 33 accessible screw thread realize dismantling the connection, be equipped with the scale on the sampling tube 33, observe the volume of taking a sample when conveniently taking a sample, sample tube 29 both ends respectively with mixing ware 12, A sampling pipe 33 is connected, wherein one end of the sampling pipe 29 extends into the mixing vessel 12 through the sampling port 21, the other end penetrates through the three-way cover 26 and extends into the sampling pipe 33, two ends of the extraction pipe 30 are respectively connected with the vacuum pump 31 and the sampling pipe 33, the length of the extraction pipe 33 extending into the sampling pipe 33 is less than the length of the sampling pipe 29 extending into the sampling pipe 33, a vacuum release valve 32 is further arranged on the three-way cover 26, when the sampling pipe 33 is connected with the three-way cover 26, the vacuum release valve 32 is connected with the sampling pipe 33 through a connecting hole of the three-way cover to protect the sampling device, a fixing piece 28 which passes through the side wall of the third sliding chute 27 and is abutted against the second bracket 25 is arranged on the third sliding chute 27, the fixing piece 28 is rotatably connected with the side wall of the third sliding chute 27 through a thread, the second bracket 25 slides in the third sliding chute 27, the sampling height can be adjusted, the fixing piece 28 is rotated after the adjustment, thereby the sampling position of the sampling device is fixed, on above basis, can also set up the control module of control vacuum pump 31 operation on sampling device to adjust vacuum pump 31's running state, realize sampling device ration, regularly take a sample, sampling device and mixing ware 12 slidable, can dismantle and be connected, make things convenient for sampling device's clearance, sterilization.

During the use, the cell sap that will wait to mix is arranged in mixing ware 12, perhaps fixed two port mixing bottle in mixing ware 12, the motor drives cam 7 and rotates, cam 7 drives gasbag 2 motion, gasbag 2 takes place elastic deformation, the air in gasbag 2 is bloated, get into mixing ware 12 through connecting pipe 10 and blast pipe 11, perhaps get into mixing bottle, drive the cell motion in the container, the motor drives cam 7 and continues to rotate, slide bar 8 loses the drive power of cam 7, gasbag 2 reconversion, inside the cell sap gets into blast pipe 11, cam 7 rotates in succession, the continuous business turn over blast pipe 11 of drive cell sap, the blowing and suction process of artifical mixing cell sap is simulated, thereby realize the mixing to the cell sap.

It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. The uniform mixing device for cell scattering is characterized by comprising a base (1), an air bag (2) and a uniform mixing vessel (12), wherein the uniform mixing vessel (12) is arranged on the base (1), a supporting rod (3) perpendicular to the base (1) is arranged at one end of the base (1), an installation seat (4) is arranged on the supporting rod (3), a cylindrical cavity (5) is arranged at one end, far away from the supporting rod (3), of the installation seat (4), the air bag (2) is arranged inside the cylindrical cavity (5), and a pressing structure for driving the air bag (2) to move is arranged inside the installation seat (4); the pressing structure comprises a first support (6), a cam (7) and a sliding rod (8), wherein the cam (7) which is rotatably connected with the first support (6) is arranged at one end, away from the cylindrical cavity (5), of the first support (6), the first sliding groove (9) which is parallel to the first support (6) is arranged at the other end of the first support (6), and the sliding rod (8) is slidably connected with the first support (6) through the first sliding groove (9); the first sliding chute (9) is arranged on the axis of the cylindrical cavity (5); one end of the cylindrical cavity (5) far away from the mounting seat (4) is provided with a connecting pipe (10), and the connecting pipe (10) is provided with an air blowing pipe (11) with one end penetrating into the mixing vessel (12).

2. The blending device for cell scattering according to claim 1, wherein the base (1) is provided with a second chute (13), and the blending dish (12) is slidably connected with the base (1) through the second chute (13).

3. The blending device for cell scattering according to claim 1, wherein the support rod (3) is further provided with a support frame (14) slidably connected with the support rod (3), and one end of the support frame (14) far away from the support rod (3) is provided with a cavity (15) matched with the air blowing pipe (11).

4. The uniform mixing device for cell scattering according to claim 1, wherein a plurality of clamping blocks (16) are uniformly arranged at one end of the cylindrical cavity (5) close to the mounting seat (4), a clamping groove (22) matched with the clamping blocks (16) is arranged on the mounting seat (4), and the cylindrical cavity (5) is detachably connected with the mounting seat (4) through the clamping blocks (16) and the clamping groove (22).

5. The blending device for cell scattering according to claim 1, wherein a ring body (17) sleeved outside the connecting pipe and the cylindrical cavity (5) is arranged at one end of the cylindrical cavity (5) far away from the mounting seat (4), and the ring body (17) is rotatably connected with the cylindrical cavity (5) through threads.

6. The blending device for cell scattering according to claim 3, wherein the side of the support frame (14) is provided with a first fastening screw (18) engaged with the support rod (3).

7. The blending device for cell disruption according to claim 1, wherein a pressing plate (19) is further disposed at one end of the sliding rod (8) close to the cylindrical cavity (5).

8. The blending device for cell scattering according to claim 1, wherein the bottom of the blending dish (12) is conical, a cover plate (20) is arranged on the blending dish (12), and a sampling port (21) is arranged on the cover plate (20).

9. The blending device for cell scattering according to claim 1, wherein one end of the mounting seat (4) close to the support rod (3) is slidably connected with the support rod (3), and a second fastening screw (23) matched with the support rod (3) is arranged on the mounting seat (4).

10. The cell scattering blending device according to claim 8, wherein a sampling device is disposed on the side of the blending dish (12), the sampling device comprises a sampling tube (33), a second bracket (25) and a vacuum pump (31), one end of the second bracket (25) is provided with a three-way cover (26), the other end of the second bracket is slidably connected with the blending dish (12) through a third chute (27) disposed on the side of the blending dish (12), the three-way cover (26) is provided with a sample inlet tube (29) and an air outlet tube (30) penetrating through the three-way cover (26), and a sampling tube (33) detachably connected with the three-way cover (26), two ends of the sample inlet tube (29) are respectively connected with the blending dish (12) and the sampling tube (33), two ends of the air outlet tube (30) are respectively connected with the vacuum pump (31) and the sampling tube (33), the three-way cover (26) is further provided with a vacuum release valve (32), and a fixing piece (28) which penetrates through the side wall of the third sliding groove (27) and is abutted against the second bracket (25) is arranged on the third sliding groove (27).