CN112852594A - Cell culture device for biotechnology research - Google Patents
Cell culture device for biotechnology research Download PDFInfo
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- CN112852594A CN112852594A CN202110079834.6A CN202110079834A CN112852594A CN 112852594 A CN112852594 A CN 112852594A CN 202110079834 A CN202110079834 A CN 202110079834A CN 112852594 A CN112852594 A CN 112852594A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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Abstract
The invention discloses a cell culture device for biotechnology research, which comprises a processing tank body, wherein a plurality of supporting feet are fixedly arranged at the bottom end of the processing tank body, the bottom ends of the supporting feet are supported on the ground, a processing cavity is arranged in the processing tank body, a tank cover plate is fixedly connected in an opening at the top end of the processing cavity through a first thread locking structure, and a driving motor is fixedly connected on the outer wall at the top end of the tank cover plate. According to the cell culture material crushing device, the arranged processing cavity, particularly the transmission rod and the stirring rod which are arranged in the processing cavity can be utilized, after the driving motor is operated, the whole stirring rod is driven to rotate, the cell culture material entering the processing cavity is fully crushed, and then the cell culture material is led into the corresponding culture drawer in batches, so that different culture materials can be conveniently and respectively researched, the whole structure design is reasonable, the operation is simple, the mechanization degree is high, and the cell culture material crushing device is worthy of later popularization and use.
Description
Technical Field
The invention belongs to the technical field of cell culture, and relates to a cell culture device for biotechnology research.
Background
Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, and the like) in vitro to enable the cell culture to survive, grow, reproduce and maintain main structure and function, also called cell cloning technology, the formal term in biology is cell culture technology, both for the whole bioengineering technology and one of the biological cloning technology, the cell culture is an indispensable process, the cell culture is large-scale cloning of cells, the cell culture technology can lead a cell to be massively cultured into simple single cells or multi-cells with few differentiation, which is an indispensable link of the cloning technology, the cell culture is cloning of the cell, the cell culture technology is an important and common technology in the research method of cell biology, a large number of cells can be obtained through cell culture, further, signal transduction of cells, anabolism of cells, growth and proliferation of cells, and the like can be studied.
In the cell culture process, in the middle of its multi-purpose culture material, can contain the reunion thing of large granule, lead to these reunion thing when follow-up cell culture, influence holistic growth efficiency and quality to traditional culture apparatus structure is too simple, leads to cultivateing the unable holding of dividing of the nourishment of cell, just also can not carry out the test of dividing the lot, reduces experimental precision, must design a cell culture apparatus for biotechnology research and solve this type of problem.
Disclosure of Invention
The invention aims to provide a cell culture device for biotechnology research, which solves the problems in the prior art: in the cell culture process, in the middle of its multi-purpose culture material, can contain the reunion thing of large granule, when leading to these reunion thing to follow-up cell culture, influence holistic growth efficiency and quality to traditional culture apparatus structure is too simple, leads to cultivateing the unable holding of dividing batch of nourishment of cell, just also can not carry out the test of dividing batch, reduces experimental precision.
In order to achieve the purpose, the invention adopts the following technical scheme:
a cell culture device for biotechnology research comprises a processing tank body, wherein a plurality of supporting feet are fixedly arranged at the bottom end of the processing tank body, the bottom ends of the supporting feet are supported on the ground, a processing cavity is arranged inside the processing tank body, a tank cover plate is fixedly connected in an opening at the top end of the processing cavity through a first thread locking structure, a driving motor is fixedly connected onto the outer wall at the top end of the tank cover plate, the output end of the driving motor is fixedly connected with a transmission rod, one end of the transmission rod penetrates through the side wall of the tank cover plate and is rotatably connected with the side wall of the tank cover plate through a rotating bearing, a plurality of stirring rods are symmetrically and fixedly connected onto the side wall of the transmission rod, a feeding assembly is arranged on the side wall of one side of the processing tank body, and an automatic stop valve II is fixedly arranged on the bottom side wall of, the one end and the inside intercommunication of processing cavity of automatic stop valve two, the one end that processing cavity was kept away from to automatic stop valve two is provided with the drawing liquid pump, the bottom mounting of drawing liquid pump is provided with supporting baseplate, the one end that supporting baseplate is close to automatic stop valve two is connected with the outer wall fixed connection of the processing jar body, the one end and the one end of automatic stop valve two of drawing liquid pump are connected with defeated material hose, the one end that automatic stop valve two was kept away from to defeated material hose is provided with the storage box, the bottom of storage box and supporting baseplate's top lateral wall fixed connection, be provided with a plurality of butt joint sleeve pipe on the lateral wall that the storage box is close to automatic stop valve two one sides, the inside of storage box is provided with cell culture storage device.
Preferably, the first thread locking structure comprises two second limiting plates which are symmetrically and fixedly connected to the outer wall of the processing tank body, two first limiting plates which are symmetrically and fixedly connected to the outer walls of the two ends of the tank cover plate are connected with each other through a fastening threaded rod.
Preferably, the feeding assembly comprises a first feeding guide pipe, one end of the first feeding guide pipe is communicated with the inside of the processing cavity, a first automatic stop valve is fixedly connected to one end, far away from the processing tank body, of the first feeding guide pipe, and a second feeding guide pipe is fixedly connected to one end, far away from the first feeding guide pipe, of the first automatic stop valve.
Preferably, the cell culture storage device comprises a plurality of injection guide pipes, one end of each injection guide pipe penetrates through one side wall of the storage box body, one end of each injection guide pipe, which extends out of the storage box body, is connected with a corresponding butt joint sleeve, one end of each delivery hose is in threaded connection with one end of one of the butt joint sleeves, a plurality of partition plates are arranged inside the storage box body, a storage chamber is formed between every two partition plates, a culture drawer is arranged in each storage chamber, one end of each culture drawer is clamped in each storage chamber, the other end of each culture drawer is fixedly connected with a sealing plate, one end, which is far away from each culture drawer, of each sealing plate is fixedly connected with a push-pull handle, a feeding plate is fixedly arranged on one side wall of each storage chamber, a feeding side pipe is arranged on the side wall of each feeding plate, and one end of each feeding side pipe is connected with a feeding bottom pipe, one end of the feeding bottom pipe extends into the material storage chamber.
Preferably, an observation window formed by tempered transparent glass is arranged on one side wall, close to the storage box body, of the processing tank body.
Preferably, two lifting handles are symmetrically and fixedly connected to the top side wall of the tank body cover plate, and anti-skidding sleeves made of rubber materials are arranged on the outer walls of the lifting handles.
The invention has the beneficial effects that: can utilize the processing cavity that sets up, especially the inside transfer line and the puddler that sets up of processing cavity, through driving motor operation back, drive whole puddler and rotate the back, carry out abundant broken handle to the cell culture material that enters into processing cavity inside, then with its leading-in the cultivation drawer that corresponds in turn, be convenient for to the different cultivation materials study respectively, holistic structural design is reasonable, easy operation, degree of mechanization is high, is worth the later stage and uses widely.
Drawings
FIG. 1 is a schematic front view of a cell culture apparatus for biotechnology research according to the present invention;
FIG. 2 is a schematic structural view of a delivery hose, a storage box, an injection conduit, a butt joint sleeve, a storage chamber and a push-pull handle;
FIG. 3 is an enlarged view of the structure A in FIG. 1;
FIG. 4 is an enlarged view of the structure B in FIG. 2;
FIG. 5 is a detailed structural diagram of the processing tank body, the tank body cover plate, the first limiting plate, the second limiting plate, the fastening threaded rod, the first feeding guide pipe, the first automatic stop valve and the second feeding guide pipe.
In the figure: the automatic feeding device comprises a processing tank body 1, a supporting bottom foot 2, a processing cavity body 3, a tank body cover plate 4, a driving motor 5, a transmission rod 6, a stirring rod 7, a first limiting plate 8, a second limiting plate 9, a fastening threaded rod 10, a lifting handle 11, a feeding guide pipe I12, an automatic stop valve I13, a feeding guide pipe II 14, an observation window 15, an automatic stop valve II 16, a liquid suction pump 17, a material conveying hose 18, a supporting bottom plate 19, a material storage box body 20, a material injection guide pipe 21, a butt joint sleeve 22, a material storage chamber 23, a culture drawer 24, a sealing plate 25, a push-pull handle 26, a feeding plate 27, a feeding side pipe 28 and a feeding bottom pipe 29.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-5, a cell culture apparatus for biotechnology research, comprising a processing tank 1, wherein a plurality of supporting feet 2 are fixedly installed at the bottom end of the processing tank 1, so as to stably support the whole processing tank 1, the bottom ends of the supporting feet 2 are supported on the ground, a processing cavity 3 is arranged inside the processing tank 1, a tank cover plate 4 is fixedly connected in an opening at the top end of the processing cavity 3 through a first thread locking structure, the first thread locking structure comprises two second limiting plates 9 symmetrically and fixedly connected to the outer wall of the processing tank 1, two first limiting plates 8 are symmetrically and fixedly connected to the outer walls at two ends of the tank cover plate 4, the first limiting plates 8 and the second limiting plates 9 are connected through a fastening threaded rod 10, and after the fastening threaded rod 10 is rotated, the first limiting plates 8 and the second limiting plates 9 are separated, conveniently take off whole jar body apron 1 from the opening at processing jar body 1 top, regularly clear up whole processing jar body 1 inside simultaneously, fixedly connected with driving motor 5 on the outer wall at jar body apron 1 top, the output fixedly connected with transfer line 6 of driving motor 5, after through driving motor operation, drive whole puddler and rotate, carry out abundant crushing treatment to the cell culture material that enters into processing cavity inside, then lead into its corresponding cultivation drawer in batches, the one end of transfer line 6 runs through the lateral wall setting of jar body apron 4, and the outer wall of transfer line 6 is connected with the lateral wall rotation of jar body apron 4 through rolling bearing, after through starting driving motor 5 operation, drive whole transfer line 6 and rotate, symmetrically fixedly connected with a plurality of puddler 7 on the lateral wall of transfer line 6, transfer line 6 drives a plurality of puddler 7 to rotate after the operation, thereby stirring the culture material inside the processing cavity 3, reducing the existence of large particles in the culture material, installing the feeding subassembly on the lateral wall of one side of the processing tank body 1, the feeding subassembly includes the first feeding conduit 12, the one end of the first feeding conduit 12 communicates with the inside of the processing cavity 3, the one end fixedly connected with the first automatic stop valve 13 that the first feeding conduit 12 keeps away from the processing tank body 1, the one end fixedly connected with the second feeding conduit 14 that the first automatic stop valve 13 keeps away from the first feeding conduit 12, cell culture is an indispensable process, cell culture itself is the large-scale cloning of cells, cell culture technique can become simple unicellular or the few differentiated multicellular through a large amount of cultivations of cells, this is the indispensable link of cloning technique, and cell culture itself is the cloning of cells, cell culture technique is important and technology in the cell biology research method commonly used, by opening the first automatic stop valve 13, culture materials are led into the processing cavity 3 through the second feeding guide pipe 14, the side wall of the bottom of the processing tank body 1 far away from one side of the feeding assembly is fixedly provided with a second automatic stop valve 16, one end of the second automatic stop valve 16 is communicated with the inside of the processing cavity 3, one end of the second automatic stop valve 16 far away from the processing cavity 3 is provided with a liquid pump 17, the bottom of the liquid pump 17 is fixedly provided with a support bottom plate 19, one end of the support bottom plate 19 close to the second automatic stop valve 16 is fixedly connected with the outer wall of the processing tank body 1, one end of the liquid pump 17 is connected with one end of the second automatic stop valve 16, one end of the liquid pump 17 far away from the second automatic stop valve 16 is connected with a material conveying hose 18, one end of the material conveying hose 18 far away from the second automatic stop valve 16, a plurality of butt joint sleeves 22 are arranged on the side wall of the storage box body 20 close to the second automatic stop valve 16, a cell culture storage device is arranged inside the storage box body 20 and comprises a plurality of injection guide pipes 21, one end of each injection guide pipe 21 penetrates through one side wall of the storage box body 20, one end of each injection guide pipe 21 extending out of the storage box body 20 is connected with the corresponding butt joint sleeve 22, one end of each material conveying hose 18 is in threaded connection with one end of one butt joint sleeve 22, a plurality of partition plates are arranged inside the storage box body 20, a storage chamber 23 is formed between each partition plate, a culture drawer 24 is arranged in each storage chamber 23, one end of each culture drawer 24 is clamped in each storage chamber 23, a seal plate 25 is fixedly connected to the other end of each culture drawer 24, and a push-pull handle 26 is fixedly connected to one end of each seal plate 25 far away from the culture drawer 24, the convenient cultivation drawer 24 that will correspond is taken out from storage compartment 23 and is used, can carry out the selective examination to the cultivation material in the middle of cultivating drawer 24 simultaneously and handle, and the effect is better, and efficiency is higher, fixed mounting has feed plate 27 on the lateral wall of storage compartment 23, be provided with feeding side tube 28 on feed plate 27's the lateral wall, feeding side tube 28's one end is connected with feeding bottom tube 29, feeding bottom tube 29's one end extends to and sets up in the storage compartment 23, be provided with the observation window 15 that toughened transparent glass constitutes on the lateral wall that the processing jar body 1 is close to storage box 20, two lifting handle 11 of symmetry fixedly connected with on the top lateral wall of jar body apron 4, be provided with the antiskid cover that rubber materials made on lifting handle 11's the outer wall, increase frictional force, make things convenient for the staff to hold behind lifting handle 11, lift whole jar body apron 4 and draw the operation.
When the culture device is used, the arranged processing cavity, particularly the transmission rod and the stirring rod arranged in the processing cavity can be utilized, after the driving motor is operated, the whole stirring rod is driven to rotate, the cell culture materials entering the processing cavity are fully crushed, and then are led into the corresponding culture drawer in batches, so that different culture materials can be conveniently and respectively researched, the whole structure is reasonable in design, the operation is simple, the mechanization degree is high, no complex operation steps are needed, and the culture device meets the current different research requirements.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The cell culture device for biotechnology research comprises a processing tank body (1) and is characterized in that a plurality of supporting feet (2) are fixedly mounted at the bottom end of the processing tank body (1), the bottom ends of the supporting feet (2) are supported on the ground, a processing cavity (3) is arranged inside the processing tank body (1), a tank cover plate (4) is fixedly connected into an opening at the top end of the processing cavity (3) through a first thread locking structure, a driving motor (5) is fixedly connected onto the outer wall at the top end of the tank cover plate (1), a transmission rod (6) is fixedly connected with the output end of the driving motor (5), one end of the transmission rod (6) penetrates through the side wall of the tank cover plate (4) and is rotatably connected with the side wall of the tank cover plate (4) through a rotating bearing, a plurality of stirring rods (7) are symmetrically and fixedly connected onto the side wall of the transmission rod (6), the feeding assembly is installed on the side wall of one side of the processing tank body (1), the automatic stop valve II (16) is fixedly installed on the bottom side wall of one side of the feeding assembly far away from the processing tank body (1), one end of the automatic stop valve II (16) is communicated with the inside of the processing cavity (3), one end of the automatic stop valve II (16) far away from the processing cavity (3) is provided with a liquid pump (17), the bottom fixing of the liquid pump (17) is provided with a supporting bottom plate (19), one end of the supporting bottom plate (19) close to the automatic stop valve II (16) is fixedly connected with the outer wall of the processing tank body (1), one end of the liquid pump (17) is connected with one end of the automatic stop valve II (16), one end of the liquid pump (17) far away from the automatic stop valve II (16) is connected with a material conveying hose (18), one end of the material conveying hose (18) far away from the automatic stop valve, the bottom of storage box body (20) and the top lateral wall fixed connection of supporting baseplate (19), be provided with a plurality of butt joint sleeve pipe (22) on the lateral wall that storage box body (20) are close to automatic stop valve two (16) one side, the inside of storage box body (20) is provided with cell culture storage device.
2. The cell culture device for biotechnological research according to claim 1, characterized in that, the first screw thread locking structure includes two second limiting plates (9) that symmetry fixed connection is on processing jar body (1) outer wall, two first limiting plates (8) that symmetry fixed connection is on the outer wall at jar body apron (4) both ends, connect through fastening threaded rod (10) between first limiting plate (8) and the second limiting plate (9).
3. The cell culture device for biotechnological research according to claim 1, characterized in that, the feed subassembly includes feed pipe one (12), the one end of feed pipe one (12) communicates with the inside of processing chamber (3), the one end of feed pipe one (12) far away from processing jar body (1) is fixedly connected with automatic stop valve one (13), the one end of automatic stop valve one (13) far away from feed pipe one (12) is fixedly connected with feed pipe two (14).
4. The cell culture device for biotechnological research according to claim 1, characterized in that, the cell culture storage device includes a plurality of material injection pipes (21), one end of the material injection pipe (21) is disposed through one side wall of the storage box (20), and one end of each material injection pipe (21) extending out of the storage box (20) is connected with the corresponding butt joint sleeve (22), one end of the material delivery hose (18) is connected with one end of one of the butt joint sleeves (22) by screw thread, the storage box (20) is provided with a plurality of partition plates, and a storage chamber (23) is formed between each partition plate, a culture drawer (24) is disposed in the storage chamber (23), one end of the culture drawer (24) is clamped in the storage chamber (23), and the other end of the culture drawer (24) is fixedly connected with a sealing plate (25), one end, far away from the cultivation drawer (24), of the sealing plate (25) is fixedly connected with a push-pull handle (26), a feeding plate (27) is fixedly mounted on one side wall of the storage chamber (23), a feeding side pipe (28) is arranged on the side wall of the feeding plate (27), one end of the feeding side pipe (28) is connected with a feeding bottom pipe (29), and one end of the feeding bottom pipe (29) extends into the storage chamber (23).
5. The cell culture device for biotechnological research according to claim 1 characterized in that a lateral wall of the processing tank (1) near the storage tank (20) is provided with an observation window (15) made of tempered transparent glass.
6. The cell culture device for biotechnological research according to claim 1, characterized in that two lifting handles (11) are symmetrically and fixedly connected to the top side wall of the tank cover plate (4), and the outer wall of the lifting handle (11) is provided with an anti-slip sleeve made of rubber material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110079834.6A CN112852594A (en) | 2021-01-21 | 2021-01-21 | Cell culture device for biotechnology research |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110079834.6A CN112852594A (en) | 2021-01-21 | 2021-01-21 | Cell culture device for biotechnology research |
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| CN112852594A true CN112852594A (en) | 2021-05-28 |
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| CN202110079834.6A Withdrawn CN112852594A (en) | 2021-01-21 | 2021-01-21 | Cell culture device for biotechnology research |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112760227A (en) * | 2021-02-19 | 2021-05-07 | 刘寿明 | Cell culture box |
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Application publication date: 20210528 |
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