CN217202706U

CN217202706U - Safe convenient fermentation cylinder feed supplement device

Info

Publication number: CN217202706U
Application number: CN202220567973.3U
Authority: CN
Inventors: 孙海彦
Original assignee: Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
Current assignee: Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
Priority date: 2022-03-16
Filing date: 2022-03-16
Publication date: 2022-08-16
Anticipated expiration: 2032-03-16

Abstract

The utility model discloses a safe convenient fermentation cylinder feed supplement device, including feed supplement bottle and fermentation cylinder, the feed supplement bottle passes through the feed supplement pipeline and is connected with the fermentation cylinder, be connected with steam pipe and exhaust pipeline on the feed supplement pipeline, be equipped with the control valve group on feed supplement pipeline, steam pipe and the exhaust pipeline. A path of independent steam pipeline is arranged to enter the material supplementing pipeline, so that independent sterilization of the valve group is guaranteed by using clean steam, sterilization is more thorough, and the risk of bacteria contamination is reduced; the multi-head material supplement can supplement one or more culture mediums at one time, a plurality of strains in microbial fermentation need to supplement the multiple culture mediums at the same time, and the equipment application range is wider.

Description

Safe convenient fermentation cylinder feed supplement device

Technical Field

The utility model relates to a fermentation technical field especially indicates a safe convenient fermentation cylinder feed supplement device.

Background

The fermentation tank is a specialized apparatus for carrying out fermentation of microorganisms, and metabolites, cells, etc. of various microorganisms can be produced by using the fermentation tank. The fermentation tank is widely applied to the fields of food, medicine, agriculture, biochemical engineering and the like. When various products of a fermentation tank organism are utilized, the material supplementing operation is often involved, the substrate inhibition effect, the glucose effect, the metabolic repression and the like can be effectively relieved through the material supplementing, the fermentation time can be prolonged, the strain is not easy to age and mutate rapidly, and the fermentation intensity and the production efficiency are finally improved. The feeding of the existing fermentation tank generally adopts a feeding insertion needle feeding method, alcohol cotton is usually placed around a feeding port, then the alcohol cotton is ignited, a feeding needle head is inserted into a feeding interface in flame, and then feeding liquid is pumped into the fermentation tank through a peristaltic pump. The problem of this kind of mode of operation is that there is open fire operation in the workshop, has the potential safety hazard.

The utility model discloses a china utility model patent that published 2019.07.16 publication number CN 209113866U did discloses a fermentation cylinder feed supplement device, including feed supplement bottle, fermentation cylinder, set up the feed supplement sterilizing equipment between feed supplement bottle and fermentation cylinder, be equipped with the steam port that lets in steam on the fermentation cylinder. However, this patent has the following drawbacks: 1. steam enters from the bottom of the fermentation tank, and gas enters the material supplementing system for sterilization after passing through a culture medium in the fermentation tank, so that the culture medium easily overflows to the material supplementing system along with the steam, and the risk of bacterial contamination is brought; 2. one end of the feed is supplemented, and only one culture medium can be supplemented at a time.

Disclosure of Invention

Not enough to in the above-mentioned background art, the utility model provides a safe convenient fermentation cylinder feed supplement device has solved among the prior art feed supplement method and has had the problem of the risk of contracting bacteria.

The technical scheme of the utility model is realized like this:

the utility model provides a safe convenient fermentation cylinder feed supplement device, includes feed supplement bottle and fermentation cylinder, the feed supplement bottle passes through the feed supplement pipeline and is connected with the fermentation cylinder, be connected with steam pipe and exhaust pipeline on the feed supplement pipeline.

Furthermore, the feed supplement pipeline comprises a fixed pipeline connected with the fermentation tank and a movable pipeline detachably connected with the fixed pipeline.

Furthermore, the movable pipeline is connected with the fixed pipeline through a clamping sleeve interface.

Further, the steam pipeline is connected with a fixed pipeline; the steam exhaust pipeline is connected with the movable pipeline.

Furthermore, the control valve group comprises an exhaust valve arranged on an exhaust pipeline, a steam valve arranged on a steam pipeline and a tank inlet valve arranged on a fixed pipeline.

Furthermore, the movable pipeline comprises a main material supplementing pipe and a multi-head material supplementing connector arranged on the main material supplementing pipe, and a plurality of material supplementing heads are arranged on the multi-head material supplementing connector.

Furthermore, the feeding bottle is detachably connected with the feeding head through a high-temperature-resistant silica gel hose which is connected with a peristaltic pump.

Furthermore, a feeding bottle cap is arranged on the feeding bottle, and the high-temperature-resistant silica gel hose penetrates through the feeding bottle cap to be fixed.

Furthermore, an air filter extending into the supplement bottle is further arranged on the supplement bottle cover.

Further, the control valve group also comprises a feed supplement valve arranged on the feed supplement main pipe.

The utility model has the advantages that: the utility model is provided with an independent steam pipeline to enter the feed supplement pipeline, which ensures that the clean steam butt joint valve set is used for independent sterilization, the sterilization is more thorough, and the contamination risk is reduced; the multi-head material supplement can simultaneously supplement one or more culture media at one time, a plurality of strains in microbial fermentation need to supplement a plurality of culture media at the same time, and the equipment has wider application range.

Drawings

In order to illustrate the embodiments of the present invention more clearly, the drawings that are needed in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the description below are only some embodiments of the present invention, and that other drawings can be obtained by those skilled in the art without inventive work.

Fig. 1 is a schematic structural diagram of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without any creative effort belong to the protection scope of the present invention.

As shown in fig. 1, the fermentation tank feeding device of embodiment 1 of the present invention comprises a feeding bottle 12 and a fermentation tank 1, wherein the feeding bottle 12 is used for containing feeding liquid; the feed supplement bottle 12 is connected with the fermentation tank 1 through a feed supplement pipeline and is used for feeding the fermentation tank 1, the feed supplement pipeline is connected with a steam pipeline 2 and a steam exhaust pipeline 15 respectively, the steam pipeline 2 is used for feeding clean steam into the feed supplement pipeline to play a role in disinfection and sterilization, the steam exhaust pipeline 15 is used for discharging steam after disinfection, and the feed supplement pipeline, the steam pipeline 2 and the steam exhaust pipeline 15 are provided with a control valve group for controlling the inlet and outlet of the steam and the feed supplement process. The valve group and the feeding pipeline are independently sterilized by clean steam through an independent steam pipeline, so that the sterilization is more thorough, and the risk of bacterial contamination is reduced.

Further, the feeding pipeline comprises a fixed pipeline connected with the fermentation tank 1 and a movable pipeline detachably connected with the fixed pipeline. Specifically, the movable pipeline is connected with the fixed pipeline through a clamping sleeve connector 4. Through setting the feed supplement pipeline into a detachable connection mode, the movable pipeline and the valve bank on the movable pipeline are conveniently placed into an autoclave for sterilization before feed supplement operation.

Further, the steam pipeline 2 is connected with a fixed pipeline; the steam exhaust pipeline 15 is connected with the movable pipeline. The control valve group comprises an exhaust valve 8 arranged on an exhaust pipeline 15, a steam valve 3 arranged on a steam pipeline 2 and a tank inlet valve 9 arranged on a fixed pipeline. Wherein, the tank inlet valve 9 is arranged at one end of the fixed pipeline close to the fermentation tank 1, and the steam pipeline 2 is connected at one end of the fixed pipeline close to the ferrule interface 4. The feed supplementing pipeline is controlled to be connected with the fermentation tank 1 through a tank inlet valve 9; the steam pipeline 2 is connected with a steam source, and the steam inlet of the steam pipeline 2 is controlled through a steam valve 3; the exhaust process on the exhaust line 14 is controlled by the exhaust valve 8. Wherein, the closing of the tank inlet valve 9 is convenient for the disinfection and sterilization. In addition, the end of the steam exhaust pipeline 15 is also provided with a cutting sleeve interface 4 for connecting the steam exhaust hose 11.

Further, the movable pipeline comprises a main material supplementing pipe and a multi-head material supplementing connector 6 arranged on the main material supplementing pipe, and a plurality of material supplementing heads 61 are arranged on the multi-head material supplementing connector 6. Because many strains need to supplement a plurality of culture mediums simultaneously in the microbial fermentation, realize once can supplementing one or more culture mediums simultaneously through a plurality of feed supplement heads 61, application range is wider. In addition, the control valve group also comprises a feed supplement valve 5 arranged on the feed supplement main pipe and used for controlling the feed supplement process.

Furthermore, the material supplementing bottle 12 is detachably connected with the material supplementing head 61 through a high-temperature-resistant silica gel hose 7 and is used for conveying material supplementing liquid, and the high-temperature-resistant silica gel hose 7 is connected with a peristaltic pump 10. The feeding liquid of the feeding bottle 12 is conveniently pumped into the fermentation tank 1 by means of a peristaltic pump 7.

Furthermore, a feeding bottle cap 14 is arranged on the feeding bottle 12 to seal the feeding bottle, and meanwhile, the high-temperature-resistant silica gel hose 7 penetrates through the feeding bottle cap 14 through a stainless steel pipe to be fixed conveniently; the high-temperature-resistant silicone hose 7 is divided into two sections which are respectively positioned on the inner side and the outer side of the material supplementing bottle 12, the stainless steel pipe penetrates through the material supplementing bottle cap 14, and two ends of the stainless steel pipe are respectively connected with the two sections of high-temperature-resistant silicone hoses 7 in a sealing mode. And, still be equipped with on feed supplement bottle lid 14 and stretch into the miniature air cleaner 13 in feed supplement bottle 12 and be used for letting in clean air in feed supplement bottle 12, guarantee the smooth progress of pump sending feed supplement liquid. In this embodiment, the air filter 13 is a teflon membrane air filter; the air filter 13 is fixed through the feeding bottle cap 14 by a stainless steel pipe.

The working process of the device is as follows:

1. before the fermentation tank 1 is fed with the feed, the feed bottle, each valve and the movable pipeline are put into an autoclave for sterilization; cooling to room temperature for later use after sterilization;

2. connecting a feed supplement valve 5, a feed supplement bottle 12, a high-temperature-resistant silicon rubber tube 7 and a feed supplement inoculation head 6 into a pipeline through a ferrule connector 4, connecting a steam exhaust hose 11 with a straight plug, tightly closing a tank inlet valve 9 and the feed supplement valve 5, opening a steam valve 3 and a steam exhaust valve 8, allowing steam to pass through a steam pipeline 2, sterilizing a valve group part, closing the steam valve 3 and the steam exhaust valve 8 after sterilizing for 30 minutes, opening the feed supplement valve 5 and the tank inlet valve 9 when the temperature of the section of pipeline and valve is reduced to room temperature, and pumping feed supplement liquid in the feed supplement bottle 12 into the fermentation tank 1 through a peristaltic pump 10;

3. after the material supplementing is finished, the tank inlet valve 9 and the material supplementing valve 5 are closed, the steam valve 3 and the steam exhaust valve 8 are opened, the pipeline is sterilized by high-pressure steam for about 30 minutes, and the steam valve 5 and the steam exhaust valve 8 are closed.

In addition, when various material supplementing operations are performed, one path of material supplementing pipe and one path of material supplementing bottle are respectively connected to the connectors 61, a certain path of material supplementing is closed in the period, the high-temperature-resistant silica gel material supplementing pipe 7 is clamped by the hemostatic clamp, and the material supplementing pipeline is cut off.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a safe convenient fermentation cylinder feed supplement device, includes feed supplement bottle (12) and fermentation cylinder (1), its characterized in that: the feed supplement bottle (12) is connected with the fermentation tank (1) through a feed supplement pipeline, the feed supplement pipeline is connected with a steam pipeline (2) and a steam exhaust pipeline (15), and control valve groups are arranged on the feed supplement pipeline, the steam pipeline (2) and the steam exhaust pipeline (15).

2. The safe and convenient fermenter feeding apparatus according to claim 1, wherein: the feed supplement pipeline comprises a fixed pipeline connected with the fermentation tank (1) and a movable pipeline detachably connected with the fixed pipeline.

3. The safe and convenient fermenter feeding device according to claim 2, wherein: the movable pipeline is connected with the fixed pipeline through a clamping sleeve joint (4).

4. The safe and convenient fermenter feeding apparatus according to claim 2 or 3, wherein: the steam pipeline (2) is connected with the fixed pipeline; the steam exhaust pipeline (15) is connected with the movable pipeline.

5. The safe and convenient fermenter feeding apparatus according to claim 4, wherein: the control valve group comprises an exhaust valve (8) arranged on an exhaust pipeline (15), a steam valve (3) arranged on a steam pipeline (2) and a tank inlet valve (9) arranged on a fixed pipeline.

6. The safe and convenient fermenter feeding apparatus of claim 2, 3 or 5, wherein: the movable pipeline comprises a material supplementing main pipe and a multi-head material supplementing connector (6) arranged on the material supplementing main pipe, and a plurality of material supplementing heads (61) are arranged on the multi-head material supplementing connector (6).

7. The safe and convenient fermenter feeding device according to claim 6, wherein: the feeding bottle (12) is detachably connected with the feeding head (61) through a high-temperature-resistant silica gel hose (7), and the high-temperature-resistant silica gel hose (7) is connected with a peristaltic pump (10).

8. The safe and convenient fermenter feeding device according to claim 7, wherein: the feeding bottle (12) is provided with a feeding bottle cap (14), and the high-temperature-resistant silica gel hose (7) penetrates through the feeding bottle cap (14) through a stainless steel pipe to be fixed.

9. The safe and convenient fermenter feeding device according to claim 8, wherein: an air filter (13) extending into the feeding bottle (12) is further arranged on the feeding bottle cap (14).

10. The safe and convenient fermenter feeding device according to any one of claims 7 to 9, wherein: the control valve group also comprises a feed supplement valve (5) arranged on the feed supplement main pipe.