CN216919273U

CN216919273U - PCR heating device

Info

Publication number: CN216919273U
Application number: CN202220681426.8U
Authority: CN
Inventors: 潘炜列; 杨鹏; 张友东; 梁威; 高长科
Original assignee: HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
Current assignee: HANGZHOU ALLSHENG INSTRUMENTS CO Ltd
Priority date: 2022-03-25
Filing date: 2022-03-25
Publication date: 2022-07-08
Anticipated expiration: 2032-03-25

Abstract

The application discloses PCR heating device relates to gene detection's technical field to it is little with PCR pipe area of contact to solve PCR heating device among the prior art, makes the inhomogeneous technical problem of PCR pipe heating. The PCR heating device comprises a rack, a mounting table and a heating block machine moving assembly; the mounting table is arranged on the rack, at least one mounting hole for placing the PCR tube is formed in the mounting table, at least one heating column is arranged on the heating block, and the heating block is used for heating the PCR tube; the moving assembly is arranged on the rack, is connected with the top surface of the heating block and is used for driving the heating block to move. Through set up a plurality of heating posts on heating block in this application, promoted the area of contact of PCR heating device with the PCR pipe for the PCR pipe can fully be heated evenly, has greatly promoted the reaction efficiency of PCR reaction.

Description

PCR heating device

Technical Field

The application relates to the technical field of gene detection, in particular to a PCR heating device.

Background

With the continuous development of medical technology in human society, molecular diagnostic techniques including PCR detection are applied more and more widely in clinical practice. In this case, a fully automatic gene detection processing system is developed.

The PCR detection module is integrated in the full-automatic gene detection processing system, a heating structure is arranged in the PCR detection module, and the heating structure needs to heat the PCR tube in the gene detection process. However, in the prior art, the heating structure is mainly a planar structure, which makes the contact area between the heating structure and the PCR tube smaller, resulting in a smaller heated area of the PCR tube, and thus reducing the detection efficiency of gene detection.

SUMMERY OF THE UTILITY MODEL

An object of this application is to provide a PCR heating device, its heating area of contact that can increase and PCR pipe increases the heated area of PCR pipe, has greatly promoted PCR reaction's reaction efficiency.

The embodiment of the application is realized as follows:

a PCR heating apparatus comprising: the device comprises a rack, a mounting table and a heating block machine moving assembly; the mounting table is arranged on the rack, and at least one first mounting hole for placing a PCR tube is formed in the mounting table; the heating block is provided with at least one heating column for heating the PCR tube; the moving assembly is arranged on the rack and connected with the top surface of the heating block and used for driving the heating block to move.

In one embodiment, the heating column is solid or hollow.

In one embodiment, a groove is formed in the bottom surface of the heating block, and the heating column is arranged on the inner bottom surface of the groove.

In an embodiment, a sealing groove is formed in an outer wall of the heating block, and the sealing groove is used for placing a heat insulation sealing element.

In one embodiment, the shape of the heating column is a cylinder, a circular truncated cone, a rectangular parallelepiped or a truncated pyramid.

In one embodiment, the heating columns are provided with a plurality of rows, each row is provided with 8 heating columns, and the number of the heating columns is the same as that of the mounting holes.

In one embodiment, the moving assembly comprises a lifting moving assembly and a horizontal moving assembly; the heating block is arranged on the lifting moving assembly and used for driving the heating block to lift; the horizontal moving component is arranged on the rack and used for driving the lifting moving component to move horizontally.

In one embodiment, the horizontal moving assembly includes a first driving member, a sliding rail, a sliding block and a mounting bracket; the first driving piece is arranged on the rack; the sliding rail is arranged on the rack; the sliding block can be movably arranged on the sliding rail; the mounting bracket is arranged on the sliding block, is in transmission connection with the first driving piece and is connected with the lifting moving assembly. The lifting moving assembly comprises a first connecting piece, a second driving piece and a plurality of guide posts; the first connecting piece is connected with the heating block, and a plurality of second mounting holes are formed in the top surface of the first connecting piece; the second driving piece is arranged on the mounting bracket and used for driving the heating block to lift; the guide post is connected on the mounting bracket and is arranged in the second mounting hole in a penetrating manner.

In one embodiment, the lifting moving assembly further comprises a spring, a heat-insulating sleeve and a fixing piece; the heat insulation sleeve is connected with the first connecting piece through the spring; the fixing piece can be detachably arranged in the heat-insulating sleeve, the heating block is arranged on the fixing piece, and the heat-insulating sleeve can be matched with the mounting table.

In one embodiment, the lifting and moving assembly further comprises a transmission member; the transmission part is arranged on the mounting bracket, is in transmission connection with the first connecting piece and is in transmission connection with the second driving piece.

Compared with the prior art, the beneficial effect of this application is:

through set up the heating post on PCR heating device's heating block in this application, increased the area of contact of PCR heating device and PCR pipe, promoted the heated area of PCR pipe to greatly promoted the reaction efficiency of PCR pipe when carrying out PCR reaction.

In addition, through setting up the heating block on removing the subassembly in this application for the heating post can be accurate and the PCR pipe aims at, promotes the heating effect to the PCR pipe.

Simultaneously, the heating post in this application can realize heating closed PCR pipe through stretching into the mode of PCR tube cap.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic cross-sectional view of a PCR heating apparatus according to an embodiment of the present application;

FIG. 2 is a schematic structural diagram of a heating block according to an embodiment of the present application;

FIG. 3 is an enlarged partial view of the portion A of the embodiment of FIG. 1 shown in an embodiment of the present application;

FIG. 4 is a schematic structural diagram of a PCR heating apparatus according to an embodiment of the present application;

FIG. 5 is a schematic cross-sectional view of a PCR heating apparatus according to an embodiment of the present application;

fig. 6 is a partially enlarged schematic view of a portion B in fig. 5 according to an embodiment of the present application.

Reference numerals:

1-PCR heating device; 10-a frame; 20-mounting a table; 21-a first mounting hole; 30-PCR tube; 40-a heating block; 410-heating column; 420-a groove; 430-seal groove; 440-a heat insulating seal; 50-a moving assembly; 51-a horizontal movement assembly; 511-a first drive member; 512-mounting a bracket; 513-a slider; 514-a slide rail; 52-a lift moving assembly; 521-a first connector; 522-second mounting hole; 523-second drive member; 524-guide post; 525-a spring; 526-insulating sleeve; 527-fixing piece; 528-transmission means.

Detailed Description

The terms "first," "second," "third," and the like are used for descriptive purposes only and not for purposes of indicating or implying relative importance, and do not denote any order or order.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should be noted that the terms "inside", "outside", "left", "right", "upper", "lower", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally arranged when products of the application are used, and are used only for convenience in describing the application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the application.

In the description of the present application, unless expressly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements.

The gene detection is a medical detection technology, which extracts nucleic acid in peripheral venous blood, tissues and other body fluids of a detected person, analyzes DNA molecule or RNA molecule information in cells of the detected person through a detection device, so as to know the gene information of the detected person and further determine the cause of disease or the risk of disease.

The nucleic acid extraction is a pretreatment process of gene detection, specific primers and probe design are carried out according to the sequence of known nucleic acid, the designed primers are synthesized, the extracted nucleic acid is used as a template to carry out a fluorescence quantitative PCR experiment, and the negative and positive of a target sample are judged according to a fluorescence signal. Nucleic acid extraction is a key step of gene detection, and the quality of the obtained nucleic acid directly influences the success or failure of downstream experiments. It should be understood that the genetic testing is not limited to the testing of human body, but also includes the genetic testing of animals and plants.

When the full-automatic gene detection processing system carries out gene detection, amplification reaction is required. In a specific mode, the PCR tube 30 is heated by the PCR heating device 1, and the heating temperature of the PCR tube 30 by the PCR heating device 1 is changed by the temperature control device, so that the reagents in the PCR tube 30 cyclically change at a plurality of temperature points. While allowing light emitted by the light emitter to enter the PCR tube 30 and exit to the sensor via the PCR tube 30. By the method, the fluorescent quantitative PCR detection based on the temperature-variable amplification technology is realized.

The technical solution of the present application will be clearly and completely described below with reference to the accompanying drawings.

Fig. 1 is a schematic cross-sectional view of a PCR heating apparatus according to an embodiment of the present application. As shown in FIG. 1, the PCR heating apparatus 1 includes a frame 10, a mounting table 20, a heating block 40, and a moving assembly 50. The mounting table 20 is arranged on the rack 10, and at least one first mounting hole 21 for placing the PCR tube 30 is arranged on the mounting table 20; the heating block 40 is provided with at least one heating column 410 for heating the PCR tube 30. Wherein, a plurality of heating elements are arranged on the heating block 40. And the moving assembly 50 is arranged on the rack 10, connected with the top surface of the heating block 40 and used for driving the heating block 40 to move. Wherein, when the cap of the PCR tube 30 is covered on the PCR tube, the heating column 410 can heat the closed PCR tube 30 by extending into the cap of the PCR tube 30.

In one embodiment, the heating column 410 is solid, and the cap of the PCR tube 30 has a slot, into which the heating column 410 can extend to heat the PCR tube 30.

In one operation, when the full-automatic gene testing system is ready for an amplification reaction, the PCR heating apparatus 1 needs to heat the PCR tube 30. Specifically, the consumable transferring device firstly places the PCR tube 30 in the first mounting hole 21 of the mounting table 20, and after the placement is completed, the moving assembly 50 drives the heating block 40 to move, so that the heating block 40 moves to the position where the PCR tube 30 is placed, and further, the heating column 410 extends into the slot of the PCR tube cover, so that the sealed PCR tube 30 is heated.

Therefore, in the present application, the heating column 410 is disposed on the heating block 40 of the PCR heating apparatus 1, so that the contact area between the PCR heating apparatus 1 and the PCR tube 30 is increased, the heating area of the PCR tube 30 is increased, and the reaction efficiency of the PCR tube 30 during PCR reaction is greatly increased. In addition, through setting up heating block 40 on removing subassembly 50 in this application for heating post 410 can be accurate and be managed 30 with PCR and aim at, promotes the heating effect to PCR pipe 30. Meanwhile, the heating column 410 in the present application can heat the closed PCR tube 30 by extending into the tube cover of the PCR tube 30.

In one embodiment, the diameter of the heating column 410 is slightly smaller than the smallest inner diameter of the slot, and the depth of the heating column 410 is slightly larger than the depth of the slot.

By the above measures, it is ensured that the lower surface of the heating column 410 can completely abut against the bottom surface of the slot when the heating column 410 extends into the slot to heat the PCR tube 30. Meanwhile, the heating column 410 can be smoothly separated from the slot after heating is finished, and the phenomenon that the heating column 410 is clamped in the slot to bring up the PCR tube 30 is avoided.

Fig. 2 is a schematic structural diagram of a heating block according to an embodiment of the present application. Please refer to fig. 3, which is a partially enlarged view of a portion a of the embodiment in fig. 1 according to an embodiment of the present disclosure. As shown in fig. 2, a groove 420 is formed on a bottom surface of the heating block 40, and a plurality of heating columns 410 are formed on an inner bottom surface of the groove 420. In addition, a sealing groove 430 is formed in the outer wall of the heating block 40, and the sealing groove 430 is used for placing a heat insulation sealing member 440, so that heat preservation, heat insulation and sealing operations of the heating block 40 are realized.

In another embodiment, the heating column 410 may be hollow, i.e., the heating column 410 is provided with a slot. At this time, the tube cover of the PCR tube 30 may be provided with a convex pillar structure, so that the convex pillar on the tube cover of the PCR tube 30 may extend into the slot of the heating column 410, thereby completing the heating operation of the PCR tube 30.

In one embodiment, the heating column 410 is in the shape of a cylinder, a truncated cone, a rectangular parallelepiped, or a truncated pyramid.

In one embodiment, the heating columns 410 are provided in a plurality of rows, and the number of the heating columns 410 is the same as the number of the second mounting holes 522. Wherein, each row can be provided with 8 heating columns 410, so that the heating columns 410 can be adapted to the tube caps of the 8-row PCR tubes 30, and the 8-row PCR tubes 30 can be heated.

In another embodiment, each row may have 24, 48, and 96 heating columns 410, etc.

Through the measures, the number of the heating columns 410 is flexibly set, so that the heating columns 410 can be matched with the tube caps of the parallel PCR tubes 30 of different models, and the heating of the PCR tubes 30 of different models is realized.

Please refer to fig. 4, which is a schematic structural diagram of a PCR heating apparatus according to an embodiment of the present application. Fig. 5 is a schematic cross-sectional view of a PCR heating apparatus according to an embodiment of the present application. The moving assembly 50 further includes a horizontal moving assembly 51 and a vertical moving assembly 52 (shown in fig. 1). Wherein, the heating block 40 is arranged on the lifting moving component 52 and used for driving the heating block 40 to lift; the horizontal moving assembly 51 is disposed on the frame 10 and is used for driving the lifting moving assembly 52 to move horizontally.

In an operation process, when the PCR tube 30 needs to be heated, the horizontal moving mechanism drives the heating block 40 to move horizontally, and when the heating block 40 moves right above the PCR tube 30, the lifting moving mechanism drives the heating block 40 to move vertically downwards until the heating column 410 can extend into the tube cover of the PCR tube 30, so as to heat the PCR tube.

In another operation process, after the PCR tube 30 is heated, the lifting mechanism drives the heating column 410 to separate from the tube cover of the PCR tube 30, and after the separation is successful, the horizontal mechanism drives the heating block 40 to separate from the tube cover of the PCR tube 30.

In one embodiment, the horizontal moving assembly 51 includes a first driving member 511, a sliding rail 514, a sliding block 513 and a mounting bracket 512. The first driving member 511 and the sliding rail 514 are disposed on the frame 10; the sliding block 513 is movably arranged on the sliding rail 514; the mounting bracket 512 is disposed on the sliding block 513, the mounting bracket 512 is in transmission connection with the first driving member 511, and the mounting bracket 512 is connected with the lifting moving assembly 52.

The lifting moving assembly 52 comprises a first connecting member 521, a second driving member 523, a plurality of guide posts 524 and a transmission member 528; wherein the first connecting member 521 is connected with the heating block 40, and a plurality of second mounting holes 522 are arranged on the top surface of the first connecting member 521; the second driving member 523 is arranged on the mounting bracket 512, and the second driving member 523 is used for driving the heating block 40 to ascend and descend; a plurality of guide posts 524 are connected to the mounting bracket 512 and pass through the second mounting holes 522; the transmission member 528 is disposed on the mounting bracket 512 and is in transmission connection with the first connecting member 521 and the second driving member 523. Also, the transmission member 528 includes a belt structure and gears.

In an operation process, the first driving member 511 drives the sliding block 513 to move along the sliding rail 514, so as to drive the mounting bracket 512 to move, further, the mounting bracket 512 drives the lifting moving assembly and the heating block 40 to move, when the heating block 40 moves right above the PCR tube 30, the second driving member 523 drives the transmission member 528 to operate, and the transmission member 528 drives the heating block 40 to vertically move downwards until the heating column 410 can extend into a tube cover of the PCR tube 30, so as to heat the PCR tube.

Please refer to fig. 6, which is a partially enlarged schematic view of a portion B in fig. 5 according to an embodiment of the present application.

As shown in fig. 6, the lifting moving assembly 52 further includes a spring 525, a thermal insulation sleeve 526 and a fixing member 527. Wherein, the insulating sleeve 526 is connected with the first connecting piece 521 through a spring 525; the fixing member 527 is detachably disposed in the insulating sleeve 526, the heating block 40 is disposed on the fixing member 527, and the insulating sleeve 526 can be engaged with the mounting table 20.

Through the measures, the heat insulation sleeve 526 can effectively realize heat insulation operation on the heating block 40, heat dissipation of the heating block 40 is reduced, and the heating effect of the heating block 40 is improved. Meanwhile, the heating block 40 is detachably arranged in the heat insulation sleeve 526, so that the heating block 40 can be conveniently replaced. The spring 525 plays a buffering role to avoid damaging the PCR tube 30.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A PCR heating apparatus, comprising:

a frame;

the mounting table is arranged on the rack, and at least one first mounting hole for placing a PCR tube is formed in the mounting table;

a heating block; the heating block is provided with at least one heating column for heating the PCR tube;

and the moving assembly is arranged on the rack, is connected with the top surface of the heating block and is used for driving the heating block to move.

2. The PCR heating apparatus as claimed in claim 1, wherein the heating column is solid or hollow.

3. The PCR heating apparatus according to claim 1, wherein a groove is provided on a bottom surface of the heating block, and the heating pin is provided on an inner bottom surface of the groove.

4. The PCR heating apparatus as claimed in claim 1, wherein a sealing groove is provided on the outer wall of the heating block, and the sealing groove is used for placing a heat insulation sealing member.

5. The PCR heating apparatus as claimed in claim 1, wherein the heating column is shaped as a cylinder, a truncated cone, a rectangular parallelepiped or a truncated pyramid.

6. The PCR heating apparatus of claim 1, wherein the heating columns are provided in a plurality of rows of 8 heating columns, and the number of the heating columns is the same as the number of the mounting holes.

7. The PCR heating apparatus of claim 1, wherein the moving assembly comprises:

the heating block is arranged on the lifting moving component and used for driving the heating block to lift;

and the horizontal moving assembly is arranged on the rack and used for driving the lifting moving assembly to move horizontally.

8. The PCR heating apparatus as claimed in claim 7, wherein the horizontal moving assembly comprises:

the first driving piece is arranged on the rack;

the sliding rail is arranged on the rack;

the sliding block is movably arranged on the sliding rail;

the mounting bracket is arranged on the sliding block, is in transmission connection with the first driving piece and is connected with the lifting moving assembly;

the lifting moving assembly comprises:

the first connecting piece is connected with the heating block, and a plurality of second mounting holes are formed in the top surface of the first connecting piece;

the second driving piece is arranged on the mounting bracket and used for driving the heating block to lift;

and the guide columns are connected to the mounting bracket and penetrate through the second mounting holes.

9. The PCR heating apparatus of claim 8, wherein the elevating movement assembly further comprises:

a spring;

the heat insulation sleeve is connected with the first connecting piece through the spring;

the fixing piece can be detachably arranged in the heat insulation sleeve, the heating block is arranged on the fixing piece, and the heat insulation sleeve can be matched with the mounting table.

10. The PCR heating apparatus of claim 9, wherein the elevating movement assembly further comprises:

and the transmission part is arranged on the mounting bracket, is in transmission connection with the first connecting piece and is in transmission connection with the second driving piece.