CN216574422U

CN216574422U - Be used for endogenetic mycorrhizal fungi separation and purification device of plant

Info

Publication number: CN216574422U
Application number: CN202122713401.XU
Authority: CN
Inventors: 邱吉茂; 成钟
Original assignee: Nanjing Cuijingyuan Biotechnology Co ltd
Current assignee: Nanjing Cuijingyuan Biotechnology Co ltd
Priority date: 2021-11-08
Filing date: 2021-11-08
Publication date: 2022-05-24
Anticipated expiration: 2031-11-08

Abstract

The utility model relates to the technical field of endophyte separation, in particular to a separation and purification device for endophyte mycorrhizal fungi, which comprises a crushing mechanism and a purification device body, wherein the upper end inside the purification device body is provided with a cleaning tank, a plurality of mesh conveyor belts are fixedly arranged inside the cleaning tank, two sides inside the cleaning tank are fixedly provided with baffle plates, the upper end of the baffle plate is fixedly provided with a plurality of spray heads, the lower end inside the cleaning tank is obliquely provided with the mesh conveyor belts, the lower end of the cleaning tank is communicated with a waste water tank, a rotating shaft drives a helical blade to extrude rootstocks to be cut through a mesh cutting plate, the rootstocks are secondarily cut through a cutting knife and enter a rolling groove, the rolling groove is driven by a hydraulic lifting rod to vertically slide on the surface of a lifting slide rail to adjust the distance between the rolling roller and the rolling roller, the rolling roller and the rolling groove are ground through a grinding block on the surface, and then a culture dish is used for receiving, the endophyte can be conveniently cultured.

Description

Be used for endogenetic mycorrhizal fungi separation and purification device of plant

Technical Field

The utility model relates to the technical field of endophyte separation, in particular to a separation and purification device for plant endophyte mycorrhizal fungi.

Background

Endophytes are a very important class of microbial resources. In order to explore these resources, particularly those endophytes present in particular habitats, the plant is often grown in a location that is remote from the relevant research base or laboratory.

The isolation operation of endophyte often needs to be through taking the plant material back to the laboratory, after disinfection and isolation handles, and the isolation culture obtains after inoculating in the culture medium under aseptic environment, needs to cut the plant material and smash, and manual operation is loaded down with trivial details, wastes time and energy, consequently needs one kind to be used for endophyte mycorrhizal fungi separation and purification device of plant to make the improvement to above-mentioned problem.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a separation and purification device for endophytic mycorrhizal fungi, which solves the problems in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme:

a separation and purification device for endophytic mycorrhizal fungi in plants comprises a crushing mechanism and a purification device body, wherein a cleaning tank is arranged at the upper end inside the purification device body, a plurality of mesh-shaped conveyor belts are fixedly arranged inside the cleaning tank, baffle plates are fixedly arranged on two sides inside the cleaning tank, a plurality of spray heads are fixedly arranged at the upper end of the baffle plates, the mesh-shaped conveyor belts are obliquely arranged at the lower end inside the cleaning tank, a waste water tank is communicated with the lower end of the cleaning tank, sterile cleaning water in the sterile cleaning water tank is used for cleaning the rhizomes of plants through the spray heads on one side by a first water pump, then the plant rhizomes are sterilized through the spray heads on the other side by a second water pump, then the waste water enters the waste water tank for recycling by the sterile cleaning water, a disinfection water tank is arranged on one side of the waste water tank, and a sterile cleaning water tank is arranged on one side of the disinfection water tank, a first water pump is fixedly arranged inside the sterile cleaning water tank and is connected with the spray header through a hose, a second water pump is fixedly arranged inside the sterile cleaning water tank and is connected with the spray header through a hose;

the crushing mechanism comprises a rotating shaft, helical blades, a cutting bin, a net-shaped cutting plate, a cutting knife, a rolling roller, a rolling groove, a lifting sliding rail and a hydraulic lifting rod.

According to the preferable scheme of the utility model, a rotating shaft is rotatably arranged in the cutting bin, the helical blade, the cutting knife and the rolling roller are fixedly connected to the surface of the rotating shaft, the helical blade is arranged in the cutting bin, the cutting bin is communicated with the cleaning tank, the upper end of the cleaning tank is hinged with a water snake waist cover plate, the cover plate is opened to place the plant roots into the upper end of the cleaning wool roller, and the cleaning wool roller rotates upwards asynchronously to drive the plant roots to turn.

According to the preferable scheme of the utility model, the bottom of the cutting bin is fixedly provided with the reticular cutting plate, the reticular cutting plate and the rolling groove are arranged in a vertically corresponding manner, the rotating shaft drives the helical blades to extrude the rootstocks to be cut through the reticular cutting plate, and the secondary cutting is carried out through the cutting knife.

As a preferable scheme of the utility model, the lifting slide rails are fixedly connected to two sides inside the purifying device body, and the rolling grooves are slidably connected to the surfaces of the lifting slide rails.

According to a preferable scheme of the utility model, a plurality of grinding blocks are fixedly arranged on the surface of the grinding roller and the inner side of the grinding groove, the grinding roller is matched with the grinding groove, the lower ends of the grinding roller and the grinding groove are arranged in a conical shape, a culture dish is correspondingly arranged at the lower end of the grinding groove, the grinding groove is driven by a hydraulic lifting rod to slide up and down on the surface of the lifting slide rail to adjust the distance between the grinding roller and the grinding roller, the grinding roller and the grinding groove are ground by the grinding blocks on the surface, and then the materials are received by the culture dish.

As the preferable scheme of the utility model, a plurality of hydraulic lifting rods are fixedly arranged at the bottom of the grinding groove, the hydraulic lifting rods are in transmission connection through a hydraulic pump, and the rotating shaft and the cleaning wool roller are in driving connection through a motor.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, plant roots and stems are placed at the upper end of the cleaning hair roller by opening the cover plate, the cleaning hair roller rotates upwards asynchronously to drive the plant roots and stems to turn over, firstly, sterile cleaning water in a sterile cleaning water tank is used for cleaning the plant roots and stems through a spray header at one side by a first water pump, then, sterile water in the sterile cleaning water tank is used for sterilizing the plant roots and stems through a spray header at the other side by a second water pump, and then, the plant roots and stems are cleaned through the sterile cleaning water, so that the pollution of sundry bacteria to endophytes extracted from plants at the later stage is avoided;

2. according to the utility model, the rotating shaft drives the helical blades to extrude rootstocks to be cut through the net-shaped cutting plate, the rootstocks are cut secondarily through the cutting knife and enter the rolling groove, the rolling groove is driven by the hydraulic lifting rod to slide up and down on the surface of the lifting slide rail to adjust the distance between the rolling groove and the rolling roller, the rolling roller and the rolling groove are ground through the grinding block on the surface, and then the culture dish is used for receiving materials, so that endophytes can be conveniently cultured.

Drawings

FIG. 1 is a schematic overall perspective view of the present invention;

FIG. 2 is a schematic view of the overall internal structure of the present invention;

FIG. 3 is a schematic top view of the cleaning tank of the present invention.

In the figure: 1. a rotating shaft; 2. a helical blade; 3. cutting the bin; 4. a net-like cutting board; 5. a cutting knife; 6. rolling a roller; 7. rolling the groove; 8. lifting the slide rail; 9. a hydraulic lifting rod; 10. a culture dish; 11. a wastewater tank; 12. an aseptic cleaning water tank; 13. a sterilizing water tank; 14. a first water pump; 15. a second water pump; 16. a mesh conveyor belt; 17. a cleaning tank; 18. a shower head; 19. cleaning the hair roller; 20. a baffle plate; 21. a cover plate; 22. a crushing mechanism; 23. a purification device body.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.

To facilitate an understanding of the utility model, the utility model will now be described more fully with reference to the accompanying drawings. Several embodiments of the utility model are presented. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1-3, the present invention provides a technical solution:

referring to fig. 1, 2 and 3, a separation and purification device for endophytic mycorrhizal fungi in plants comprises a crushing mechanism 22 and a purification device body 23, a cleaning tank 17 is arranged at the upper end inside the purification device body 23, a plurality of mesh conveyor belts 16 are fixedly arranged inside the cleaning tank 17, baffle plates 20 are fixedly arranged on two sides inside the cleaning tank 17, a plurality of spray heads 18 are fixedly arranged at the upper end of the baffle plates 20, the mesh conveyor belts 16 are obliquely arranged at the lower end inside the cleaning tank 17, a waste water tank 11 is communicated with the lower end of the cleaning tank 17, a disinfection water tank 13 is arranged on one side of the waste water tank 11, an aseptic cleaning water tank 12 is arranged on one side of the disinfection water tank 13, a first water pump 14 is fixedly arranged inside the aseptic cleaning water tank 12, the first water pump 14 is connected with the spray heads 18 through a hose, a second water pump 15 is fixedly arranged inside the disinfection water tank 13, the second water pump 15 is connected with the spray heads 18 through a hose, and the aseptic cleaning water in the aseptic cleaning water tank 12 is firstly used for plant roots through the spray heads 18 on one side Cleaning the stems, then sterilizing the plant roots by sterilizing water in a sterilizing water tank 13 through a spray header 18 on the other side by a second water pump 15, then cleaning by sterile cleaning water, and recycling waste water in a waste water tank 11;

the crushing mechanism 22 comprises a rotating shaft 1, helical blades 2, a cutting bin 3, a net-shaped cutting plate 4, a cutting knife 5, a rolling roller 6, a rolling groove 7, a lifting slide rail 8 and a hydraulic lifting rod 9.

In the embodiment, please refer to fig. 1, 2 and 3, the inside rotation of cutting bin 3 is equipped with rotation axis 1, helical blade 2 and cutting knife 5, the equal fixed connection of rolling roller 6 is in rotation axis 1 surface, helical blade 2 sets up inside cutting bin 3, cutting bin 3 sets up with washing tank 17 UNICOM, washing tank 17 upper end hinged joint water snake waist apron 21, rotation axis 1 drives 2 extrusion rhizomes of helical blade and cuts through netted cutting board 4, cuts through cutting knife 5 secondary cutting.

Referring to fig. 1, 2 and 3, a net-shaped cutting plate 4 is fixedly arranged at the bottom of the cutting bin 3, and the net-shaped cutting plate 4 and the rolling groove 7 are arranged in an up-and-down corresponding manner.

In an embodiment, referring to fig. 1, 2 and 3, the lifting slide rails 8 are fixedly connected to two sides inside the purifying device body 23, and the rolling grooves 7 are slidably connected to the surfaces of the lifting slide rails 8.

In the embodiment, referring to fig. 1, 2 and 3, a plurality of grinding blocks are fixedly arranged on the surface of the grinding roller 6 and on the inner side of the grinding groove 7, the grinding roller 6 is matched with the grinding groove 7, the lower ends of the grinding roller 6 and the grinding groove 7 are arranged in a conical shape, a culture dish 10 is correspondingly arranged at the lower end of the grinding groove 7, the grinding groove 7 is driven by a hydraulic lifting rod 9 to slide up and down on the surface of a lifting slide rail 8 to adjust the distance between the grinding roller 6 and the grinding groove 7, the grinding roller 6 and the grinding groove 7 are ground by the grinding blocks on the surface, and then the materials are received by the culture dish 10.

In the embodiment, referring to fig. 1, 2 and 3, the bottom of the rolling groove 7 is fixedly provided with a plurality of hydraulic lifting rods 9, the hydraulic lifting rods 9 are connected through hydraulic pump transmission, and the rotating shaft 1 and the cleaning wool roller 19 are connected through motor drive.

The working principle is as follows: when the device is used, the cover plate 21 is opened, plant roots and stems are placed at the upper end of the cleaning wool roller 19, the cleaning wool roller 19 rotates upwards asynchronously to drive the plant roots and stems to turn over, firstly, sterile cleaning water in the sterile cleaning water tank 12 is cleaned through the spray head 18 on one side through the first water pump 14, then, sterile water in the sterilizing water tank 13 is sterilized through the spray head 18 on the other side through the second water pump 15, then, the plant roots and stems are cleaned through the sterile cleaning water, wastewater enters the wastewater tank 11 to be recovered, at the moment, the cleaning wool roller 19 rotates downwards to enable the roots and stems to be separated from the cleaning wool roller 19 to fall on the mesh conveyor belt 16 and then are led into the cutting bin 3, the rotating shaft 1 drives the helical blade 2 to extrude the roots and stems to be cut through the mesh cutting plate 4, the secondary cutting is carried out through the cutting knife 5 and then enters the rolling groove 7, the rolling groove 7 is driven by the hydraulic lifting rod 9 to slide up and down on the surface of the lifting slide rail 8 to adjust the distance between the rolling groove 6, the rolling roller 6 and the rolling groove 7 are ground by the grinding blocks on the surface, and then received by the culture dish 10.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a be used for endogenetic mycorrhizal fungi separation and purification device of plant, includes rubbing crusher constructs (22) and purification device body (23), its characterized in that: a cleaning tank (17) is arranged at the upper end inside the purifying device body (23), a plurality of mesh-shaped conveyor belts (16) are fixedly arranged inside the cleaning tank (17), a baffle (20) is fixedly arranged on two sides in the cleaning tank (17), a plurality of spray headers (18) are fixedly arranged at the upper end of the baffle (20), a mesh-shaped conveyor belt (16) is obliquely arranged at the lower end in the cleaning tank (17), a waste water tank (11) is communicated with the lower end of the cleaning tank (17), a disinfection water tank (13) is arranged at one side of the waste water tank (11), an aseptic cleaning water tank (12) is arranged at one side of the disinfection water tank (13), a first water pump (14) is fixedly arranged inside the sterile cleaning water tank (12), the first water pump (14) is connected with the spray header (18) through a hose, the second water pump (15) is fixedly arranged inside the disinfection water tank (13), and the second water pump (15) is connected with the spray header (18) through a hose;

the crushing mechanism (22) comprises a rotating shaft (1), helical blades (2), a cutting bin (3), a net-shaped cutting plate (4), a cutting knife (5), a rolling roller (6), a rolling groove (7), a lifting slide rail (8) and a hydraulic lifting rod (9).

2. The separation and purification device for the endophytic mycorrhizal fungi of the plant according to claim 1, characterized in that: cutting storehouse (3) inside rotation is equipped with rotation axis (1), and helical blade (2) and cutting knife (5), the equal fixed connection in rotation axis (1) surface of roller (6) rolls, and helical blade (2) set up inside cutting storehouse (3), and cutting storehouse (3) and washing tank (17) UNICOM set up, and washing tank (17) upper end hinged joint water snake waist apron (21).

3. The separation and purification device for the endophytic mycorrhizal fungi of the plant according to claim 1, characterized in that: the bottom of the cutting bin (3) is fixedly provided with a reticular cutting plate (4), and the reticular cutting plate (4) and the rolling groove (7) are arranged up and down correspondingly.

4. The separation and purification device for the endophytic mycorrhizal fungi of the plant according to claim 1, characterized in that: lifting slide rail (8) fixed connection is in purification device body (23) inside both sides, rolls groove (7) sliding connection in lifting slide rail (8) surface.

5. The separation and purification device for the endophytic mycorrhizal fungi of the plant according to claim 1, characterized in that: roll roller (6) surface and roll groove (7) inboard and all fix and be equipped with a plurality of abrasive brick, roll roller (6) and roll groove (7) looks adaptation, and roll roller (6) and roll groove (7) lower extreme and be the toper setting, roll groove (7) lower extreme correspondence and be equipped with culture dish (10).

6. The separation and purification device for the endophytic mycorrhizal fungi of the plant according to claim 1, characterized in that: the bottom of the grinding groove (7) is fixedly provided with a plurality of hydraulic lifting rods (9), the hydraulic lifting rods (9) are connected through hydraulic pump transmission, and the rotating shaft (1) and the cleaning wool roller (19) are connected through motor driving.