CN216337697U - Full-automatic nucleic acid extraction element of bulky - Google Patents
Full-automatic nucleic acid extraction element of bulky Download PDFInfo
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- CN216337697U CN216337697U CN202122337719.2U CN202122337719U CN216337697U CN 216337697 U CN216337697 U CN 216337697U CN 202122337719 U CN202122337719 U CN 202122337719U CN 216337697 U CN216337697 U CN 216337697U
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Abstract
The utility model relates to a large-volume full-automatic nucleic acid extraction device, which comprises a liquid transferring mechanical arm, a nucleic acid extraction mechanism and a reagent consumable storage mechanism, wherein the liquid transferring mechanical arm is used for transferring and conveying reagent consumables stored in the reagent consumable storage mechanism to the nucleic acid extraction mechanism; the nucleic acid extraction mechanism comprises a magnetic rod and a magnetic sleeve which are vertically arranged, the magnetic rod is positioned above the magnetic sleeve and can be inserted into the magnetic sleeve from the top end of the magnetic sleeve, the magnetic rod and the magnetic sleeve are respectively and fixedly connected with a nucleic acid extraction mechanical arm, the nucleic acid extraction mechanical arm drives the magnetic rod and the magnetic sleeve to move, a container frame is arranged below the magnetic sleeve and is connected with a displacement power source, and the displacement power source drives the container frame to move under the magnetic sleeve. The large-volume full-automatic nucleic acid extraction device provided by the technical scheme adopts a scheme of combining a liquid transfer workstation and a magnetic rod sleeve, solves the timeliness and flexibility of large-volume nucleic acid extraction, and greatly improves the automation degree of large-volume nucleic acid extraction.
Description
Technical Field
The utility model belongs to the technical field of detection equipment, and particularly relates to a large-volume full-automatic nucleic acid extraction device.
Background
The current novel clinical molecular diagnosis technology mainly comprises nucleic acid detection and gene detection. They all need to extract nucleic acid DNA/RNA from various samples, and the nucleic acid detection is to examine viral DNA/RNA in the samples, and is limited by the existing commercial extraction devices, and the detection sample volume is usually low and does not exceed 200ul, thereby limiting the extension of the detection mode and limiting the detection sensitivity. The gene detection is to perform big data analysis and comparison by checking DNA/RNA in a sample, generally, the gene detection requires a larger sample volume, and the extraction process requires a larger holding volume of a reaction solution, is also influenced by the existing commercialization, and has a lower automation degree at present.
For example, the novel coronavirus covi-19 virus is a single-stranded positive-sense RNA virus wrapped by protein, the genetic material RNA of the new coronavirus is a direct evidence of the existence of the virus, the fluorescence quantitative PCR nucleic acid detection technology is used for differential diagnosis of diseases by specifically detecting the viral RNA, the existing fluorescence quantitative PCR nucleic acid detection method for the new coronavirus needs to extract RNA from a sample as a template for PCR, under the characteristics of high risk and quick infection, large-batch rapid screening is an important means for inhibiting virus diffusion, but the existing RNA extraction technology and device are a main bottleneck in the rapid extraction process of the RNA template, and in addition, the method is limited by the existing extraction method, including the extraction of a sample with an insufficiently large volume, especially a sample with low copy nucleic acid content, and the RNA yield is low, so that the differential diagnosis cannot be accurately performed.
In the prior art, a magnetic bead method is mostly adopted for nucleic acid extraction, the sample volume applicable to a high-throughput automation scheme is usually low (50-200ul), and the automation degree of nucleic acid extraction with a high sample volume is low, and large-volume processing is usually completed manually.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects of the prior art, the utility model provides a large-volume full-automatic nucleic acid extraction device.
The utility model solves the technical problems through the following technical means: the large-volume full-automatic nucleic acid extraction device comprises a liquid transfer mechanical arm, a nucleic acid extraction mechanism and a reagent consumable storage mechanism, wherein the liquid transfer mechanical arm is used for transferring and conveying the reagent consumables stored in the reagent consumable storage mechanism to the nucleic acid extraction mechanism;
the nucleic acid extraction mechanism comprises a magnetic rod and a magnetic sleeve which are vertically arranged, the magnetic rod is positioned above the magnetic sleeve and can be inserted into the magnetic sleeve from the top end of the magnetic sleeve, the magnetic rod and the magnetic sleeve are respectively and fixedly connected with a nucleic acid extraction mechanical arm, the nucleic acid extraction mechanical arm drives the magnetic rod and the magnetic sleeve to move, a container frame is arranged below the magnetic sleeve and is connected with a displacement power source, and the displacement power source drives the container frame to move under the magnetic sleeve.
As the improvement of the technical scheme, the interior of the reagent consumable storage mechanism is divided into a reagent area and a Tips head area.
As an improvement of the technical scheme, the reagent area comprises a reagent tank carrier, and a reagent tank is placed on the reagent tank carrier.
As an improvement of the technical scheme, the reagent zone further comprises a tube carrier, and the tube carrier is of a structure that a plurality of blind holes are formed in a metal block from top to bottom.
As an improvement to the above solution, the Tips head section comprises Tips head carriers and carrier bays, the Tips head carriers being fixed on the carrier bays.
As an improvement of the technical scheme, the waste material area is also arranged and is a cavity with an open top.
The utility model has the beneficial effects that: the full-automatic nucleic acid extraction element of bulky volume that this technical scheme provided adopts liquid-transfering work station + bar magnet shell type to combine together the scheme, and liquid-transfering work station carries out bulky sample and solution and shifts, and the bar magnet cover accomplishes the nucleic acid and draws fast, and the timeliness and the flexibility that bulky nucleic acid drawed are solved to this scheme to the degree of automation that bulky nucleic acid drawed has been improved greatly.
Drawings
Fig. 1 is a schematic structural view of the present invention.
FIG. 2 is a schematic view of the structure of the nucleic acid isolation mechanism of the present invention.
Fig. 3 is an enlarged view of fig. 1 at a.
Fig. 4 is an enlarged view of fig. 1 at B.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.
Examples
As shown in fig. 1-4, the large-volume fully automatic nucleic acid extracting apparatus according to this embodiment includes a liquid-transferring mechanical arm, a nucleic acid extracting mechanism, and a reagent consumable storing mechanism, wherein the liquid-transferring mechanical arm is used for transferring the reagent consumable stored in the reagent consumable storing mechanism to the nucleic acid extracting mechanism;
the nucleic acid extraction mechanism comprises a magnetic rod 1 and a magnetic sleeve 2 which are vertically arranged, wherein the magnetic rod 1 is positioned above the magnetic sleeve 2, the magnetic rod 1 can be inserted into the magnetic sleeve from the top end of the magnetic sleeve 2, the magnetic rod 1 and the magnetic sleeve 2 are respectively and fixedly connected with a nucleic acid extraction mechanical arm, the nucleic acid extraction mechanical arm drives the magnetic rod 1 and the magnetic sleeve 2 to move, a container rack 4 is arranged below the magnetic sleeve 2, a displacement power source 3 is connected with the container rack 4, and the displacement power source 3 drives the container rack 4 to move under the magnetic sleeve 2.
The full-automatic nucleic acid extraction element of bulky volume that this technical scheme provided adopts liquid-transfering work station + bar magnet shell type to combine together the scheme, and liquid-transfering work station carries out bulky sample and solution and shifts, and the bar magnet cover accomplishes the nucleic acid and draws fast, and the timeliness and the flexibility that bulky nucleic acid drawed are solved to this scheme to the degree of automation that bulky nucleic acid drawed has been improved greatly.
The reagent consumable storage mechanism is internally divided into a reagent area and a Tips head area.
Reagent district includes reagent groove carrier 5, reagent groove 6 has been placed on the reagent groove carrier 5, and reagent groove 6 is independent adaptive volume setting, can take according to the demand is nimble, when effectively utilizing the space, practices thrift the cost of different volume consumptive material holding greatly.
The reagent zone also comprises a tube carrier 7, and the tube carrier 7 is a structure with a plurality of blind holes formed in the metal block from top to bottom. The bottom of the tube carrier 7 can be provided with a refrigeration module, and the tube carrier 7 is made of metal with good heat conductivity.
The Tips head region includes Tips head carriers 8 and carrier floors 9, the Tips head carriers 8 being fixed on the carrier floors 9. Tips head carrier 8 is used for the location to carry the Tips head, is convenient for move the liquid mechanical arm and takes.
Still be provided with the waste material district, the waste material district is the open cavity in top. Used for collecting waste liquid and waste gun heads.
The specific using steps are as follows:
s1: the preparation before the experiment, including reagent, consumptive material, loads in appointed position.
S2: the sample is placed on a sample tube carrier, which can place 1-96 sample tubes, which are loaded into the sample zone.
S3: the pipetting mechanical arm automatically takes Tips heads, and transfers samples and reagents to the deep-hole plate from the sample area and the reagent area in sequence.
S4: the automatic nucleic acid extraction device in the extraction area completes mixing, cracking, extraction and purification.
S5: and the pipetting mechanical arm transfers the PCR reaction system construction reagent in the reagent area to the eight-connected tube in the reaction construction system area, and transfers the purified nucleic acid in the deep-hole plate to the eight-connected tube. Or the PCR reaction system construction reagent in the reagent area is transferred to the deep-hole plate, and then the reaction construction system in the deep-hole plate is transferred to the eight-connected tube.
S6: and collecting and treating the waste.
It is noted that, in this document, relational terms such as first and second, and the like, if any, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (6)
1. Full-automatic nucleic acid extraction element of bulky, its characterized in that: the device comprises a liquid transfer mechanical arm, a nucleic acid extraction mechanism and a reagent consumable storage mechanism, wherein the liquid transfer mechanical arm is used for transferring and conveying the reagent consumables stored in the reagent consumable storage mechanism to the nucleic acid extraction mechanism;
the nucleic acid extraction mechanism comprises a magnetic rod (1) and a magnetic sleeve (2) which are vertically arranged, wherein the magnetic rod (1) is positioned above the magnetic sleeve (2), the magnetic rod (1) can be inserted into the magnetic sleeve (2) from the top end of the magnetic sleeve, the magnetic rod (1) and the magnetic sleeve (2) are respectively and fixedly connected with a nucleic acid extraction mechanical arm, the nucleic acid extraction mechanical arm drives the magnetic rod (1) and the magnetic sleeve (2) to move, a container frame (4) is arranged below the magnetic sleeve (2), the container frame (4) is connected with a displacement power source (3), and the displacement power source (3) drives the container frame (4) to move under the magnetic sleeve (2).
2. The apparatus for extracting nucleic acid according to claim 1, wherein: the reagent consumable storage mechanism is internally divided into a reagent area and a Tips head area.
3. The apparatus for extracting nucleic acid according to claim 2, wherein: the reagent zone comprises a reagent tank carrier (5), and a reagent tank (6) is placed on the reagent tank carrier (5).
4. The apparatus for extracting nucleic acid according to claim 2, wherein: the reagent zone also comprises a tube carrier (7), and the tube carrier (7) is of a structure that a plurality of blind holes are formed in a metal block from top to bottom.
5. The apparatus for extracting nucleic acid according to claim 2, wherein: the Tips head region comprises Tips head carriers (8) and carrier floors (9), the Tips head carriers (8) being fixed on the carrier floors (9).
6. The apparatus for extracting nucleic acid according to claim 2, wherein: still be provided with the waste material district, the waste material district is the open cavity in top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202122337719.2U CN216337697U (en) | 2021-09-26 | 2021-09-26 | Full-automatic nucleic acid extraction element of bulky |
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| CN202122337719.2U CN216337697U (en) | 2021-09-26 | 2021-09-26 | Full-automatic nucleic acid extraction element of bulky |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410427A (en) * | 2022-01-21 | 2022-04-29 | 湖南博朔生物科技有限公司 | Large-volume full-automatic nucleic acid extraction method and device |
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2021
- 2021-09-26 CN CN202122337719.2U patent/CN216337697U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410427A (en) * | 2022-01-21 | 2022-04-29 | 湖南博朔生物科技有限公司 | Large-volume full-automatic nucleic acid extraction method and device |
| CN114410427B (en) * | 2022-01-21 | 2024-05-28 | 湖南博朔生物科技有限公司 | Large-volume full-automatic nucleic acid extraction method and device |
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