CN219689719U - Nucleic acid extraction and amplification integrated system and kit - Google Patents
Nucleic acid extraction and amplification integrated system and kit Download PDFInfo
- Publication number
- CN219689719U CN219689719U CN202320494984.8U CN202320494984U CN219689719U CN 219689719 U CN219689719 U CN 219689719U CN 202320494984 U CN202320494984 U CN 202320494984U CN 219689719 U CN219689719 U CN 219689719U
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- acid extraction
- kit
- temperature control
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 72
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 72
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 72
- 238000000605 extraction Methods 0.000 title claims abstract description 58
- 230000003321 amplification Effects 0.000 title claims abstract description 55
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 55
- 238000001514 detection method Methods 0.000 claims abstract description 52
- 230000033001 locomotion Effects 0.000 claims abstract description 36
- 230000003287 optical effect Effects 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims description 52
- 239000011324 bead Substances 0.000 claims description 30
- 238000010828 elution Methods 0.000 claims description 23
- 238000002156 mixing Methods 0.000 claims description 15
- 238000000746 purification Methods 0.000 claims description 13
- 239000012188 paraffin wax Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 238000012197 amplification kit Methods 0.000 claims description 4
- 238000002788 crimping Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 31
- 238000012546 transfer Methods 0.000 abstract description 17
- 239000000284 extract Substances 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 7
- 238000012864 cross contamination Methods 0.000 abstract description 6
- 238000004458 analytical method Methods 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 238000004140 cleaning Methods 0.000 description 8
- 238000003752 polymerase chain reaction Methods 0.000 description 7
- 238000001917 fluorescence detection Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 238000012123 point-of-care testing Methods 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The utility model discloses a nucleic acid extraction and amplification integrated system and a kit, comprising: a horizontal movement device; the temperature control device is arranged on the horizontal moving device; the optical detection device is arranged normal to the movement direction of the horizontal movement device; and the connector is arranged above the horizontal moving device through the vertical moving device. The system can be compatible with most nuclear extraction and amplification systems, is flexible in setting, simple in system operation, small in size and high in automation degree, can run in a full-automatic process by only manually adding samples, is free of liquid operation in the whole process, does not need to manually transfer extracts, avoids cross contamination, can effectively reduce the risk of false positive results, truly realizes the detection and analysis of nucleic acid entering and exiting samples, and is particularly suitable for non-specific PCR laboratory environments in emergency treatment, middle and small hospitals and the like, and no professional requirements are met for operators.
Description
Technical Field
The utility model relates to the technical field of detection equipment of biological and medical reagents, in particular to a nucleic acid extraction and amplification integrated system and a kit.
Background
Nucleic acid extraction and Polymerase Chain Reaction (PCR) are common analytical means for molecular biology. Conventionally, nucleic acid extraction and PCR have been performed in separate instruments, and after sample pretreatment, an experimenter needs to manually extract and purify nucleic acid or on a nucleic acid extractor, and after the completion of nuclear extraction, manually prepare a PCR experiment system and then detect the nucleic acid on a QPCR instrument. The whole process takes longer time. In addition, the separate processes of nucleic acid extraction and purification and PCR amplification detection are easy to cause cross contamination and even environmental pollution, and cause false positives of detection results, and influence the accuracy of the results. Based on this, many multi-core amplification integrated systems are emerging on the market. The system has a vertical or table type full-automatic system with high throughput and high throughput, and also has POCT equipment for fast detection. Large-scale full-automatic systems, most of which are huge and require special sites; POCT rapid detection equipment, suitable scene is wider, and the use is convenient, but present POCT rapid detection equipment mostly adopts consumptive materials such as disposable cartridge, chip of comparatively complicacy, adopts the reagent system of closure, and the suitability is not wide, has also restricted the application relatively.
In view of this, how to make the system integrating nucleic acid extraction and PCR amplification detection into one body, its consumable is simple, the reagent system applicability is wide, can be compatible with most nuclear extraction amplification systems, and can also reduce the cross contamination at the same time and solve the technical problem of the utility model. Therefore, there is a need for an integrated nucleic acid extraction and amplification system and kit that at least partially address the problems of the prior art.
Disclosure of Invention
In the summary, a series of concepts in a simplified form are introduced, which will be further described in detail in the detailed description. The summary of the utility model is not intended to define the key features and essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
To at least partially solve the above problems, the present utility model provides an integrated nucleic acid extraction and amplification system and a kit, comprising:
the horizontal moving device is used for placing, supporting and horizontally conveying the kit;
the temperature control device is arranged on the horizontal moving device and is used for providing a required temperature for the acid extraction reaction, elution and detection in the kit;
the optical detection device is arranged normal to the movement direction of the horizontal movement device and is used for optically detecting the nucleic acid in the kit;
the connector is arranged above the horizontal moving device through the vertical moving device and is used for carrying out the moving operation of the nucleic acid extracting solution and the magnetic beads by matching with the pipetting head and the magnetic sleeve in the kit.
Preferably, the horizontal movement device is arranged on a horizontally arranged guide rail, and the horizontal movement device is connected with the driving device through a screw rod.
Preferably, the horizontal moving device is provided with a crimping mechanism for compressing and fixing the reagent kit.
Preferably, the temperature control device is located inside the horizontal movement device, and comprises a reaction position temperature control module for providing a reaction temperature required by a nucleic acid extraction reaction, an elution position temperature control module for providing a temperature required by an elution operation, and an amplification temperature control module for providing a temperature required by a nucleic acid detection, wherein temperature sensors are arranged in the reaction position temperature control module, the elution position temperature control module and the amplification temperature control module.
Preferably, the vertical moving device is arranged on a guide rail which is arranged vertically, and a driving device is arranged on the vertical moving device.
Preferably, the vertical moving device is provided with a piston and a piston driving device for driving the piston to move, the connecting head is provided with a vertical through hole, the piston is arranged in the through hole of the connecting head, and the bottom of the piston is provided with a magnetic rod.
Preferably, the optical detection device is composed of at least one group of excitation light sources, a filter lens group and a detection device.
The utility model provides a nucleic acid extraction and amplification kit which is used for a nucleic acid extraction and amplification integrated system and comprises a nucleic acid extraction and purification card strip and an amplification reaction strip;
the nucleic acid extraction and purification clamping strip is detachably connected with the amplification reaction strip;
a pipetting head tube and a mixing tube with a pipetting head are arranged on the nucleic acid extraction purification clamping strip;
at least one reaction tube with solid paraffin preset inside is arranged on the amplification reaction strip.
Preferably, the nucleic acid extraction and purification clamping strip is further provided with a magnetic sleeve with a built-in magnetic sleeve, at least one cleaning tube, an eluting tube, a reserved tube and magnetic beads, wherein the magnetic beads are arranged in the mixing tube or the reserved tube.
Compared with the prior art, the utility model at least comprises the following beneficial effects:
the temperature control device can provide the temperature required by nucleic acid extraction, reaction, elution and nucleic acid detection, and can feed back the detected temperature to the circuit control system for temperature control and adjustment.
The connector on the vertical moving device can be matched with the pipetting head on the kit and the inner wall of the magnetic sleeve to perform pipetting operation of the pipetting head or the magnetic sleeve. And the liquid is transferred by the liquid transfer head, and the magnetic bead system is transferred by the magnetic sleeve, so that the system can finish two operations (liquid transfer by the liquid transfer head and magnetic bead transfer by the magnetic sleeve) by one set of mechanism. The vertical moving device can be matched with the horizontal moving device to move, so that when the magnetic sleeve is used, the connector can be connected with the magnetic sleeve, and the magnetic bead system can be rotated and transferred at each position in the kit; when the liquid-transferring head is used, the connector can be connected with the liquid-transferring head and transfer liquid through the liquid-transferring head.
The optical detection device can be arranged on the optical detection movement mechanism, so that the system in the amplification reaction tube of the kit is subjected to fluorescence detection or color development detection under the drive of the optical detection movement mechanism;
the optical detection device can be fixed, and the optical detection device is arranged normal to the movement direction of the horizontal movement device, so that the amplification reaction tube of the kit can sequentially pass through the optical detection device through the movement of the horizontal movement device, and the system in the amplification reaction tube is subjected to fluorescence detection;
the temperature, movement, optical detection, signal acquisition and transmission and the like of the whole system can be controlled by a circuit control system.
The automatic operation flow of the system is as follows:
1. delivering the kit out of the bin by the horizontal moving device, and putting the kit on the horizontal moving device after the kit tears the film;
2. the connector is moved in the magnetic sleeve, and the magnetic sleeve is arranged;
3. the horizontal moving device and the vertical moving device are mutually matched to move, and the magnetic beads are moved into the reserved tube to the mixing tube;
4. when the mixing tube is uniformly mixed, the vertical moving device drives the magnetic sleeve to move up and down through the connecting head to uniformly mix the system, and the temperature control device controls the temperature in the reaction tube;
5. the connector is used for carrying out the cleaning operation by carrying out the magnetic beads which are cracked, combined and coated with nucleic acid in the mixing tube, adsorbing and moving the magnetic beads to the cleaning tube;
6. transferring the cleaned magnetic beads to an elution tube by a connector for elution operation;
7. the connector withdraws the magnetic sleeve into the magnetic sleeve, and then the connector is provided with a pipetting head at a pipetting head pipe;
8. a liquid transferring head connected with the connector removes the nucleic acid extraction finished product liquid from the eluting tube and fills the liquid into the amplification reaction tube;
9. the temperature control device heats up, controls the temperature in the amplification reaction tube, and the optical detection device performs fluorescence detection;
10. after the experiment is finished, the temperature control device is cooled.
Through the design of the structure, the system can be compatible with most nuclear extraction and amplification systems, is flexible in arrangement, simple in system operation, small in size and high in automation degree, can be operated in a full-automatic flow by only manually adding samples, is free of liquid operation in the whole process, does not need to manually transfer extracts, avoids cross contamination, can effectively reduce the risk of false positive results, truly realizes the nucleic acid detection analysis of sample inlet and sample outlet, and is particularly suitable for non-specific PCR laboratory environments such as emergency treatment, medium and small hospitals and the like, and no professional requirement is required for operators.
The system is matched with the kit to adopt double settings of a pipetting head and a magnetic sleeve, the magnetic sleeve is convenient for flexibly setting an amplification flow, and is compatible with a plurality of types of nuclear extraction reagents of a plurality of systems. The liquid-transferring head can accurately and reliably transfer the nuclear extract liquid into the nucleic acid detection reaction tube, the applicability is wider, and the system integration and the customer customization are facilitated.
Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the utility model.
Drawings
The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:
FIG. 1 is a schematic diagram of the integrated system for nucleic acid extraction and amplification according to the present utility model.
FIG. 2 is a cross-sectional view of an integrated nucleic acid extraction and amplification system according to the present utility model.
FIG. 3 is a partial cross-sectional view of a nucleic acid extraction amplification integrated system according to the present utility model in pipetting.
FIG. 4 is a sectional view showing three states of the pipette head mounting (A), pipetting (B) and separation (C) according to the utility model.
Fig. 5 is a sectional view showing three states of the magnetic sleeve mounting (a), the adsorbing (B) and the separating (C) according to the present utility model. .
FIG. 6 is a schematic flow chart (S1-S6) of the integrated nucleic acid extraction and amplification system according to the present utility model.
In the figure: 100 reagent box, 200 liquid transferring head, 300 magnetic sleeve, 1 horizontal moving device, 2 temperature control device, 3 optical detecting device, 4 vertical moving device, 5 connector, 6 crimping mechanism, 7 piston, 8 piston driving device, 9 magnetic rod.
Detailed Description
The present utility model is described in further detail below with reference to the drawings and examples to enable those skilled in the art to practice the utility model by referring to the description.
It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 1 to 6, the present utility model provides an integrated nucleic acid extraction and amplification system comprising: the horizontal moving device 1 is used for placing, supporting and horizontally conveying the kit;
the temperature control device 2 is arranged on the horizontal movement device 1 and is used for providing a required temperature for the acid extraction reaction, elution and detection in the kit;
an optical detection device 3 which is arranged normal to the movement direction of the horizontal movement device 1 and is used for optical detection of nucleic acid in the kit;
the connector 5 is arranged above the horizontal moving device 1 through the vertical moving device 4 and is used for carrying out the moving operation of the nucleic acid extracting solution and the magnetic beads by matching with the pipetting head and the magnetic sleeve in the kit.
The technical scheme has the working principle and beneficial effects that: the temperature control device 2 can provide the temperature required by nucleic acid extraction, cleavage and combination reaction, elution and nucleic acid detection, and can feed back the detected temperature to a circuit control system for temperature control and adjustment.
The connector 5 on the vertical moving device 4 can be matched with the pipetting head on the kit and the inner wall of the magnetic sleeve to perform pipetting operation of the pipetting head or the magnetic sleeve. And the liquid is transferred by the liquid transfer head, and the magnetic bead system is transferred by the magnetic sleeve, so that the system can finish two operations (liquid transfer by the liquid transfer head and magnetic bead transfer by the magnetic sleeve) by one set of mechanism. The vertical moving device 4 can be matched with the horizontal moving device 1 to move, so that when the magnetic sleeve is used, the connector 5 can be connected with the magnetic sleeve, and the magnetic bead system can be rotated and transferred at each position in the kit; when a pipetting head is used, the connection head 5 can be connected to the pipetting head and the transfer of liquid takes place via the pipetting head.
The optical detection device 3 can be arranged on the optical detection movement mechanism, so that the system in the amplification reaction tube of the kit is subjected to fluorescence detection or color development detection under the drive of the optical detection movement mechanism;
the optical detection device 3 can be fixed, and because the optical detection device is arranged normal to the movement direction of the horizontal movement device 1, the amplification reaction tube of the kit can sequentially pass through the optical detection device 3 through the movement of the horizontal movement device 1, so that the system in the amplification reaction tube can be subjected to fluorescence detection;
the temperature, movement, optical detection, signal acquisition and transmission and the like of the whole system can be controlled by a circuit control system.
The automatic operation flow of the system is as follows:
1. the horizontal moving device 1 is taken out of the warehouse, and the reagent box is placed on the horizontal moving device 1 after the reagent box is torn off the film;
2. the connector 5 is moved in the magnetic sleeve and is provided with the magnetic sleeve;
3. the horizontal moving device 1 and the vertical moving device 4 are mutually matched to move, and magnetic beads are moved into the reserved pipe to the mixing pipe;
4. when the mixing pipes are uniformly mixed, the vertical moving device 4 moves up and down with the magnetic sleeve through the connector 5 to uniformly mix the systems, and the temperature control device 2 controls the temperature in the reaction pipes;
5. the connector 5 is used for carrying out cleaning operation on the magnetic beads which are subjected to cracking, combination and coating of nucleic acid in the mixing tube, and the magnetic beads are adsorbed and removed to the cleaning tube;
6. the cleaned magnetic beads are transferred to an elution tube by a connector 5 for elution operation;
7. the magnetic sleeve is retracted into the magnetic sleeve by the connector 5, and then the liquid-transferring head is arranged at the liquid-transferring head pipe by the connector 5;
8. the liquid transferring head connected with the connector 5 removes the nucleic acid extracting finished product liquid from the eluting tube and fills the liquid into the amplification reaction tube;
9. the temperature control device 2 heats up to control the temperature in the amplification reaction tube, and the optical detection device 3 performs fluorescence detection;
10. after the experiment is finished, the temperature control device 2 is cooled.
Through the design of the structure, the system can be compatible with most nuclear extraction and amplification systems, is flexible in arrangement, is simple in system operation, small in size and high in automation degree, can be operated in a full-automatic flow by only manually adding samples, is free of liquid operation in the whole process, does not need to manually transfer extracts, avoids cross contamination, can effectively reduce the risk of false positive results, and can truly realize the nucleic acid detection analysis of sample inlet and sample outlet, in addition, the system is particularly suitable for non-specific PCR laboratory environments such as emergency treatment, medium and small hospitals and the like, and has no professional requirements on operators;
the system is matched with the kit to adopt double settings of a pipetting head and a magnetic sleeve, the magnetic sleeve is convenient for flexibly setting an amplification flow, and is compatible with a plurality of types of nuclear extraction reagents of a plurality of systems. The liquid-transferring head can accurately and reliably transfer the nuclear extract liquid into the nucleic acid detection reaction tube, the applicability is wider, and the system integration and the customer customization are facilitated.
In one embodiment, the horizontal movement device 1 is arranged on a horizontally arranged guide rail, and the horizontal movement device 1 is connected by a screw and a drive device. The horizontal moving device 1 is provided with a crimping mechanism 6 for compacting and fixing the reagent kit. The temperature control device 2 is located inside the horizontal movement device 1, and comprises a reaction position temperature control module for providing a reaction temperature required by a nucleic acid extraction reaction, an elution position temperature control module for providing a temperature required by an elution operation, and an amplification temperature control module for providing a temperature required by a nucleic acid detection, wherein temperature sensors are arranged in the reaction position temperature control module, the elution position temperature control module and the amplification temperature control module. The vertical moving device 4 is arranged on a guide rail which is vertically arranged, and a driving device is arranged on the vertical moving device 4. The vertical moving device 4 is provided with a piston 7 and a piston driving device 8 for driving the piston 7 to move, the connector 5 is provided with a vertical through hole, the piston 7 is arranged in the through hole of the connector 5, and the bottom of the piston 7 is provided with a magnetic rod 9. The optical detection device 3 is composed of at least one group of excitation light sources, a filter lens group and a detection device.
The technical scheme has the working principle and beneficial effects that: through the design of above-mentioned structure, piston 7 passes through piston drive 8 drive, and piston 7 reciprocates in the through-hole of connector 5 in order to compress or inflation air to make the liquid inlet and discharge of pipette head realization liquid, the bottom of piston 7 is bar magnet 9, and in the bar magnet 9 can the upper and lower motion entering magnetic sleeve under the drive of piston 7, along with bar magnet 9 gets into in the magnetic sleeve for the magnetic bead can adsorb on the magnetic sleeve, thereby realizes the transfer of magnetic bead system.
When the pipetting head or the magnetic sleeve is required to be separated from the connector 5, the piston driving device 8 drives the piston 7 to penetrate through the connector 5 and move downwards all the time, and when the magnetic rod 9 at the bottom of the piston 7 contacts the inner wall of the pipetting head or the magnetic sleeve, the piston 7 continuously moves downwards and pushes the pipetting head or the magnetic sleeve, so that the pipetting head or the magnetic sleeve can be separated from the connector 5, as shown in fig. 4 and 5.
The automated operation flow is shown in fig. 6:
s1: the connector 5 moves to the reserved pipe with the magnetic sleeve, the piston driving device 8 drives the piston 7 to move downwards until the magnetic rod 9 abuts against the bottom of the magnetic sleeve, and the magnetic beads are adsorbed on the magnetic sleeve under the magnetic force of the magnetic rod 9;
s2: the magnetic sleeve adsorbed with the magnetic beads is moved to the mixing pipe by the connector 5, the magnetic rod 9 moves upwards to enable the magnetic beads to fall into the mixing pipe from the magnetic sleeve, the magnetic sleeve moves up and down to mix a system, and meanwhile, the reaction position temperature control module heats the mixing pipe to accelerate the reaction speed;
s3: the magnetic rod 9 descends to the bottom of the magnetic sleeve, adsorbs the magnetic bead system and moves to the cleaning pipe for cleaning operation;
s4: transferring the cleaned magnetic beads to an elution tube by a magnetic sleeve, and performing elution operation, wherein the elution position temperature control module heats the elution tube;
s5: the connector 5 withdraws the magnetic sleeve into the magnetic sleeve, then a liquid-transferring head is arranged at the liquid-transferring head pipe, the liquid-transferring head moves into the elution pipe, the piston 7 moves upwards, and the nucleic acid extraction finished product liquid is sucked into the liquid-transferring head;
s6: the liquid transferring head moves downwards into the reaction tube and passes through the middle hole of the solid paraffin ring in the reaction tube, the piston 7 moves downwards to fill the finished liquid in the liquid transferring head into the reaction tube, the reaction is carried out, the optical detection is carried out in the reaction process, meanwhile, the temperature of the amplification temperature control module is increased, after the temperature in the reaction tube exceeds the melting point of the solid paraffin, the solid paraffin is melted into liquid, and the system in the reaction tube is sealed.
After the experiment is finished, the temperature increasing and controlling module stops heating, the paraffin melted into liquid becomes solid, and the system is sealed.
The system is simple to operate, small in size and high in automation degree, the system can be operated in a full-automatic flow mode only by manually adding samples, the whole process instrument is in liquid-free operation, extracts are not required to be manually transferred, cross contamination is avoided, false positive risks of results are reduced to a certain extent, nucleic acid detection and analysis of sample inlet and outlet are truly realized, and in addition, the system is particularly suitable for non-specific PCR laboratory environments of emergency treatment, medium and small hospitals and the like, and meanwhile, no professional requirements are required for operators;
the utility model adopts the double arrangement of the pipetting head and the magnetic sleeve, the magnetic sleeve is convenient for flexibly arranging the amplification flow, and is compatible with a plurality of systems and a plurality of kinds of nuclear stripping reagents. The liquid-transferring head can accurately and reliably transfer the nuclear extract liquid into the nucleic acid detection reaction tube, the applicability is wider, and the system integration and the customer customization are facilitated.
A nucleic acid extraction and amplification kit is used for the nucleic acid extraction and amplification integrated system and comprises a nucleic acid extraction and purification card strip and an amplification reaction strip;
the nucleic acid extraction and purification clamping strip is detachably connected with the amplification reaction strip;
a pipetting head tube and a mixing tube with a pipetting head are arranged on the nucleic acid extraction purification clamping strip;
at least one reaction tube with solid paraffin preset inside is arranged on the amplification reaction strip.
The nucleic acid extraction purification clamping strip is also provided with a magnetic sleeve, at least one cleaning pipe, an eluting pipe, a reserved pipe and magnetic beads, wherein the magnetic sleeve is internally provided with a magnetic sleeve, and the magnetic beads are arranged in the mixing pipe or the reserved pipe.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Although embodiments of the present utility model have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the utility model would be readily apparent to those skilled in the art, and accordingly, the utility model is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.
Claims (9)
1. A nucleic acid extraction amplification integrated system, comprising:
the horizontal moving device (1) is used for placing, supporting and horizontally conveying the kit;
the temperature control device (2) is arranged on the horizontal moving device (1) and is used for providing a required temperature for the acid extraction reaction, elution and detection in the kit;
the optical detection device (3) is arranged normal to the movement direction of the horizontal movement device (1) and is used for optically detecting the nucleic acid in the kit;
the connector (5) is arranged above the horizontal moving device (1) through the vertical moving device (4) and is used for carrying out the moving operation of the nucleic acid extracting solution and the magnetic beads by matching with the pipetting head and the magnetic sleeve in the kit.
2. The integrated system for nucleic acid extraction and amplification according to claim 1, wherein the horizontal movement device (1) is provided on a horizontally disposed rail, and the horizontal movement device (1) is connected by a screw and a driving device.
3. The integrated nucleic acid extraction and amplification system according to claim 1, wherein the horizontal moving device (1) is provided with a crimping mechanism (6) for compressing and fixing the kit.
4. The integrated system for nucleic acid extraction and amplification according to claim 1, wherein the temperature control device (2) is located inside the horizontal movement device (1), and comprises a reaction site temperature control module for providing a reaction temperature required for a nucleic acid extraction reaction, an elution site temperature control module for providing a temperature required for an elution operation, and an amplification temperature control module for providing a temperature required for nucleic acid detection, wherein temperature sensors are provided in the reaction site temperature control module, the elution site temperature control module, and the amplification temperature control module.
5. The integrated nucleic acid extraction and amplification system according to claim 1, wherein the vertical movement device (4) is provided on a vertically arranged guide rail, and a driving device is provided on the vertical movement device (4).
6. The integrated system for nucleic acid extraction and amplification according to claim 1, wherein a piston (7) and a piston driving device (8) for driving the piston (7) to move are arranged on the vertical moving device (4), a vertical through hole is arranged on the connector (5), the piston (7) is arranged in the through hole of the connector (5), and a magnetic rod (9) is arranged at the bottom of the piston (7).
7. The integrated system for nucleic acid extraction and amplification according to claim 1, wherein the optical detection device (3) is composed of at least one group of excitation light sources, a filter lens group, and a detection device.
8. A nucleic acid extraction amplification kit for use in the integrated nucleic acid extraction amplification system of any one of claims 1-7, comprising a nucleic acid extraction purification cartridge and an amplification reaction cartridge;
the nucleic acid extraction and purification clamping strip is detachably connected with the amplification reaction strip;
a pipetting head tube and a mixing tube with a pipetting head are arranged on the nucleic acid extraction purification clamping strip;
at least one reaction tube with solid paraffin preset inside is arranged on the amplification reaction strip.
9. The nucleic acid extraction and amplification kit according to claim 8, wherein the nucleic acid extraction and purification strip is further provided with a magnetic sleeve with a built-in magnetic sleeve, at least one washing tube, an eluting tube, a reserved tube and magnetic beads, wherein the magnetic beads are arranged in the mixing tube or the reserved tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320494984.8U CN219689719U (en) | 2023-03-15 | 2023-03-15 | Nucleic acid extraction and amplification integrated system and kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320494984.8U CN219689719U (en) | 2023-03-15 | 2023-03-15 | Nucleic acid extraction and amplification integrated system and kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219689719U true CN219689719U (en) | 2023-09-15 |
Family
ID=87945103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320494984.8U Active CN219689719U (en) | 2023-03-15 | 2023-03-15 | Nucleic acid extraction and amplification integrated system and kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219689719U (en) |
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2023
- 2023-03-15 CN CN202320494984.8U patent/CN219689719U/en active Active
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