CN216274192U - Cell culture system for microscope - Google Patents
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- CN216274192U CN216274192U CN202122714888.3U CN202122714888U CN216274192U CN 216274192 U CN216274192 U CN 216274192U CN 202122714888 U CN202122714888 U CN 202122714888U CN 216274192 U CN216274192 U CN 216274192U
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Abstract
The cell culture system for a microscope of the present invention includes: cultivate box, glass lid, activity bolt, temperature and humidity detector, mixed steam entry, aqua storage tank, it sets up the activity bolt to cultivate box one side, and the glass lid can the block set up temperature and humidity detector at cultivation box top on the inner wall of the box, and mixed steam entry sets up on cultivateing the box, mixed steam entry and cultivation box inside intercommunication, and cultivation box inner wall bottom a week is provided with the aqua storage tank, cultivates the box and settles on microscope objective table. Cultivate the box and set up movable bolt for to inserting various pipelines in the box, insert the humiture in the mixed steam regulation culture box that the configuration is good through the mixed steam entry, humiture data is used for the feedback in the humiture detector surveys the culture box, and the comdenstion water that produces on cultivateing the box inner wall is collected to the aqua storage tank, and the glass lid is convenient for observe, reduces the influence of the external world to the cell culture environment. The microscope is matched with a microscope for installation, is convenient for observation at any time, has high integration level and is beneficial to operation.
Description
Technical Field
The utility model belongs to the field of cell culture devices, and particularly relates to a cell culture system for a microscope.
Background
Cell culture refers to a technique of simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to grow and reproduce cells or biological tissues and maintain their main structures and functions. At present, in scientific research work taking in vitro cultured cells as research objects, the cell culture technology and the microscopic imaging technology are generally required to be simply and conveniently combined. The physiological indices of the cells were studied while maintaining a normal cell culture environment.
At present, there are commercial cell culture devices that can be used with a microscope for scientific research, and there are mainly the following products: 1. BioStudio-T's living cell culture analysis system from NiKon, Japan. The system places the imaging system in the CO2In the incubator, the incubator provides environmental conditions required for cell growth, and the imaging system performs real-time tracking imaging on the biological sample. The disadvantage of this system is that it has a large limitation on the imaging system and is complicated to operate and prone to contamination of the cells. 2. STX series desktop incubator of TOKAIHIT, Japan. The system adopts a microenvironment chamber top plate heater to control the temperature of the culture environment, adopts an internal humidification mode and ensures that a high humidity environment is kept in the microenvironment chamber; digital CO built in controller2A gas mixer. The disadvantages of this system are: a isThe temperature distribution in the culture box is uneven and has large difference; b. in the long-term culture process, water in the culture box needs to be supplemented at irregular time to ensure enough humidity; c. the control unit has a complicated structure and a high equipment price. 3. Cage incubator from OKO Lab, Italy. The system adopts the temperature unit to generate hot air so as to improve the temperature inside the incubator and ensure the culture temperature in the cavity; mixing CO by means of a built-in gas mixing device2And O2To obtain a stable gas environment within the incubation chamber, a high humidity environment is provided by connecting to the active humidity module through a gas line. The disadvantages of this system are: a. the equipment operation is complex, the number of accessories is large, and the whole volume is large; b. the price is high; c. the humidity probe is arranged outside the culture cavity and cannot accurately reflect the temperature of the cell culture environment.
At present, relevant cell culture devices are also reported in research. Velve-Casquillas G reports a microfluidic temperature control device, a microfluidic chip used by the device is of a double-layer structure, a bottom channel, namely a cell channel, is used for cell culture, and the cell channel structure can be designed according to requirements. Above the Cell channel is a temperature control channel to which water is delivered through the thermoelectric module by pushing and pulling a syringe pump, controlling the system temperature (Velve-Casquillas G, et al. methods in Cell Biology,2010, 97, 185-. Futai N has devised a hand-held microfluidic chip cell culture system that allows temperature control by means of a flexible transparent heater attached to the lower surface of the slide, which is assembled on a fixed plate to ensure the stability of the chip. The medium is pretreated to dissolve carbonate in it, so that the cells growing on carbonate do not need 5% CO supplied from outside2Gas conditions (Futai N, et al. Lab Chip,2006, 6, 149-. Although the cell micro-culture systems are small in size and low in cost, the problems of complex operation, single function and the like generally exist in the cell micro-culture systems. Some cell culture devices are also proposed in a plurality of patents, but the device has single function and cannot be matched with various types of microscopes well to carry out related imaging research. For example: the yellow Bo et al proposed a stacked sealed cell culture cassette (CN 201999918U); a cell is providedA culture box (CN 211689094U); a cell culture apparatus (CN 112812966A) was proposed.
In summary, the existing commercial cell micro-culture system generally has the problems of complex operation, large volume, inconvenience in carrying, assembly and disassembly, high price and the like. The cell micro-culture system proposed by the research report and the related patent generally has the problems of complex operation, single function and the like.
SUMMERY OF THE UTILITY MODEL
Accordingly, the present invention is directed to solving the above-mentioned disadvantages of the prior art and to providing a cell culture system for a microscope.
Therefore, the technical scheme is that the cell culture system for the microscope comprises: cultivate box, glass lid, activity bolt, temperature and humidity detector, mixed steam entry, aqua storage tank, it sets up to cultivate box one side the activity bolt, the glass lid can the block be in cultivate the box top, set up on the cultivation box inner wall temperature and humidity detector, mixed steam entry sets up cultivate on the box, mixed steam entry and the inside intercommunication of cultivation box, cultivation box inner wall bottom a week is provided with the aqua storage tank, it settles on microscope objective table to cultivate the box.
Preferably, the temperature and humidity control system includes: the device comprises a temperature and humidity control box, a power switch, a first air inlet, a second air inlet, a mixed steam output interface, a first flow meter, a second flow meter, a steam heating bottle, a water changing port, a mixed steam output pipe, a mixed gas input pipe, a temperature and humidity detector interface, a thermistor and a heating fan interface;
the power switch is arranged on the temperature and humidity control box, the first air inlet and the second air inlet are arranged on a box body of the temperature and humidity control box, the first air inlet is connected with the input end of the first flow meter through a pipeline, the second air inlet is connected with the input end of the second flow meter through a pipeline, the output end of the first flow meter and the output end of the second flow meter are respectively communicated with one end of the mixed gas input pipe, the other end of the mixed gas input pipe is communicated with the steam heating bottle, the steam heating bottle is communicated with one end of the mixed steam output pipe, the other end of the mixed steam output pipe is communicated with the mixed steam output interface, the mixed steam output interface is arranged on the temperature and humidity control box, and the mixed steam output interface is communicated with the mixed steam inlet through a pipeline, the first flowmeter and the second flowmeter are arranged on the temperature and humidity control box, the steam heating bottle is arranged in the temperature and humidity control box, the water changing port is arranged at the top of the steam heating bottle, and the heating fan port is arranged below the temperature and humidity detector port of the culture box;
the temperature and humidity detector interface of the culture box is connected with the temperature and humidity detector and the thermistor through connecting wires, the thermistor is installed above the culture box, and the middle part of the connecting wire of the thermistor is fixed on the outer wall of the culture box.
Preferably, the temperature and humidity control box is provided with a temperature adjusting button, a culture box humidity display screen, a culture box temperature display screen, a thermistor temperature display screen and a heat radiation fan, the temperature adjusting button is electrically connected with the steam heating bottle, the heating fan interface and the heat radiation fan, the culture box humidity display screen and the culture box temperature display screen are electrically connected with the temperature and humidity detector interface,
and the thermistor temperature display screen is electrically connected with the temperature and humidity detector interface.
Preferably, the microscope body is built on the incubator, the microscope objective table is installed inside the incubator, the incubator is composed of a plurality of side plates, two top plates and two bottom plates, the adjacent side plates are fixed through buckles, the top and the bottom in the incubator are provided with fixing beams along the edges, the fixing beams pass through screw rods and are multiple, the side plates are fixedly connected with the bottom plates, a heating fan is installed on one side of the incubator, the heating fan is electrically connected with the heating fan interface, and a wind shield is arranged at the air outlet of the heating fan.
Preferably, incubator bottom and a plurality of bracing piece screwed connection, be equipped with the ratchet on the bracing piece, the bracing piece sets up in the stay tube, the bracing piece with stay tube sliding connection, stay tube upper portion is close to one side pipe wall of ratchet is opened there is the square hole, the vertical face in square hole is articulated with the pole of perk, perk pole one end with the ratchet block, the other end of perk pole is connected with compression spring's one end, the compression spring other end is connected with the boss, the boss sets up the square hole lower extreme, the boss and with the stay tube outside is connected.
Preferably, the left side plate and the right side plate are provided with door plates, the door plates are hinged with the side plates through hinges, one corner of the door plate, which is far away from the hinges, is embedded with a magnetic buckle, the left side plate and the right side plate are correspondingly provided with adsorption points and are in adsorption connection with the door plates, and door handles are fixed on the outer sides of the door plates.
Preferably, a shading shell is arranged on the incubator and consists of four left shell plates and four right shell plates, every two of the left shell plates are fixed through screw fixing pieces, the joint of the three left shell plates is clamped through a diagonal socket, every two of the right shell plates are fixed through the screw fixing pieces, the joint of the three right shell plates is clamped through the diagonal socket, and the joint of the left shell plate and the right shell plate is fixed through a plurality of magnets in an adsorption mode.
Preferably, the culture cassette is made of a photosensitive resin material.
Preferably, the temperature and humidity control box is made of a resin material.
Preferably, the incubator and the fixed beam are made of acrylic materials.
The technical scheme of the utility model has the following advantages: cultivate the box setting the activity bolt for to insert various pipelines in the cultivation box, through the mixed steam entry inserts the mixed steam who disposes and adjusts humiture in the cultivation box, temperature and humidity detector records humiture data is used for the feedback in the cultivation box, the aqua storage tank is collected after the steam condensation the comdenstion water that produces on the cultivation box inner wall, and accessible slope the cultivation box is followed the comdenstion water activity bolt department flows, the glass lid is convenient for observe, reduces the influence of external world to the cell culture environment. The microscope is matched with a microscope for installation, is convenient for observation at any time, has high integration level, is beneficial to operation, and utilizes the microscope to carry out imaging separation.
Drawings
The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model. In the drawings:
FIG. 1 is a schematic view of a culture cassette of the present invention;
FIG. 2 is a schematic view of the temperature and humidity control system of the present invention;
FIG. 3 is a schematic view of a steam heating bottle of the present invention;
FIG. 4 is a schematic view of an incubator of the present invention;
FIG. 5 is a partial structural view of the bottom of the incubator of the present invention
FIG. 6 is a schematic view of a light blocking housing of the present invention;
wherein, 1-a culture box, 2-a glass box cover, 3-a movable bolt, 4-a temperature and humidity detector, 5-a mixed steam inlet, 6-a water storage tank, 7-a temperature and humidity control box, 8-a power switch, 9-a first air inlet, 10-a second air inlet, 11-a mixed steam output interface, 12-a first flow meter, 13-a second flow meter, 14-a steam heating bottle, 15-a water exchange port, 16-a mixed steam output pipe, 17-a mixed gas input pipe, 18-a temperature and humidity detector interface, 19-a thermistor, 20-a heating fan interface, 21-a temperature adjusting button, 22-a culture box humidity display screen, 23-a culture box temperature display screen, and 24-a thermistor temperature display screen, 25-radiator fan, 26-incubator, 27-side plate, 28-top, 29-top plate, 30-buckle, 31-fixed beam, 32-heating fan, 33-wind shield, 34-door plate, 35-hinge, 36-magnetic buckle, 37-door handle, 38-shading shell, 39-left shell plate, 40-right shell plate, 41-screw fixing piece, 41-diagonal socket, 43-magnet, 44-screw rod, 45-supporting rod, 46-supporting tube, 47-ratchet bar, 48-square hole, 49-warp bar, 50-compression spring and 51-lug boss.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly or indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and is therefore not to be construed as limiting the utility model.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The present invention provides a cell culture system for a microscope, as shown in FIGS. 1 to 6, comprising: cultivate box 1, glass lid 2, activity bolt 3, temperature and humidity detector 4, mixed steam entry 5, aqua storage tank 6, it sets up to cultivate 1 one side of box movable bolt 3, glass lid 2 can the block be in cultivate 1 top of box, it sets up on the box inner wall to cultivate 1 temperature and humidity detector 4, mixed steam entry 5 sets up cultivate on the box 1, mixed steam entry 5 with cultivate 1 inside intercommunication of box, it is provided with aqua storage tank 6 to cultivate 1 inner wall bottom a week of box, it settles on microscope objective table to cultivate box 1.
The working principle and the beneficial effects of the technical scheme are as follows: cultivate box 1 and set up movable bolt 3 for to insert various pipelines in the cultivation box 1, through mixed steam inlet 5 inserts the mixed steam regulation of configuration the humiture in the cultivation box 1, humiture detector 4 surveys the humiture data is used for the feedback in the cultivation box 1, the aqua storage tank 6 is collected the steam condensation after the comdenstion water that produces on the 1 inner wall of cultivation box, and accessible slope it follows the comdenstion water to cultivate box 1 activity bolt 3 department flows, glass lid 2 is convenient for observe, reduces the influence of external world to the cell culture environment. The microscope is matched with the microscope for installation, is convenient for observation at any time, has high integration level, is beneficial to operation, and utilizes the microscope for imaging separation.
In one embodiment, a temperature and humidity control system comprises: the temperature and humidity control box 7, the power switch 8, the first air inlet 9, the second air inlet 10, the mixed steam output interface 11, the first flowmeter 12, the second flowmeter 13, the steam heating bottle 14, the water changing port 15, the mixed steam output pipe 16, the mixed gas input pipe 17, the temperature and humidity detector interface 18, the thermistor 19 and the heating fan interface 20;
the power switch 8 is arranged on the temperature and humidity control box 7, the first air inlet 9 and the second air inlet 10 are arranged on the box body of the temperature and humidity control box 7, the first air inlet 9 is connected with the input end of the first flowmeter 12 through a pipeline, the second air inlet 10 is connected with the input end of the second flowmeter 13 through a pipeline, the output end of the first flowmeter 12 and the output end of the second flowmeter 13 are respectively communicated with one end of the mixed gas input pipe 17, the other end of the mixed gas input pipe 17 is communicated with the steam heating bottle 14, the steam heating bottle 14 is communicated with one end of the mixed steam output pipe 16, the other end of the mixed steam output pipe 16 is communicated with the mixed steam output interface 11, and the mixed steam output interface 11 is arranged on the temperature and humidity control box 7, the mixed steam output interface 11 is communicated with the mixed steam inlet 5 through a pipeline, the first flowmeter 12 and the second flowmeter 13 are arranged on the temperature and humidity control box 7, the steam heating bottle 14 is arranged in the temperature and humidity control box 7, the top of the steam heating bottle 14 is provided with the water changing port 15, and the heating fan interface 20 is arranged below the temperature and humidity detector interface 18 of the culture box 1.
Cultivate the humiture detector interface 18 of box 1 with humiture detector 4 with thermistor 19 passes through the connecting wire and connects, thermistor 19 is installed cultivate box 1 top, thermistor 19's connecting wire middle part is fixed cultivate on the box 1 outer wall.
The working principle and the beneficial effects of the technical scheme are as follows: in the case of conventional cell culture, the first air inlet 10 is connected with filtered air, and the second air inlet 11 is connected with 99.999% CO2The mixing ratio of the two gases is controlled by the first flow meter 12 and the second flow meter 13 to sufficiently meet the culture environment requirements of different cells, the mixed gas is conveyed into the steam heating bottle 14 through the gas mixed gas input pipe 17, the water in the bottle is heated by the steam heating bottle 14 to generate steam, the steam is mixed with the mixed gas, the mixed steam is conveyed to the mixed steam output interface 11 through the mixed steam output interface 11, the mixed steam is conveyed to the mixed steam inlet 5 through a pipeline through the mixed steam output interface 11, the water changing port 15 can replace or add the water in the steam heating bottle 14, and the thermistor 19 measures the temperature change near the outside of the culture box 1.
In one embodiment, temperature control box 7 is provided with temperature regulation button 21, cultivates box humidity display screen 22, cultivates box temperature display screen 23, thermistor temperature display screen 24, radiator fan 25, temperature regulation button 21 with steam heating bottle 14 heating fan interface 20 radiator fan 25 electricity is connected, cultivates box humidity display screen 22 cultivates box temperature display screen 23 with temperature and humidity detector interface 18 electricity is connected, thermistor temperature display screen 24 with temperature and humidity detector interface 18 electricity is connected.
In one embodiment, a microscope body is built on an incubator 26, a microscope stage is installed inside the incubator 26, the incubator 26 is composed of a plurality of side plates 27, two top plates 28 and two bottom plates 29, adjacent side plates 27 are fixed through buckles 30, fixing beams 31 are arranged on the edges of the top and the bottom in the incubator 26, the fixing beams 31 are fixedly connected with the side plates 27 and the bottom plates 29 through screws 44, a heating fan 32 is installed on one side of the incubator 26, the heating fan 32 is electrically connected with the heating fan interface 20, and a wind shield 33 is arranged at an air outlet of the heating fan 32.
In one embodiment, the bottom of the incubator 26 is connected with a plurality of support rods 45 through screws, a ratchet rack 47 is arranged on each support rod 45, each support rod 45 is arranged in a support tube 46, each support rod 45 is connected with the corresponding support tube 46 in a sliding manner, a square hole 48 is formed in a tube wall of one side, close to the ratchet rack 47, of the upper portion of each support tube 46, a vertical surface of each square hole 48 is hinged to a tilting rod 49, one end of each tilting rod 49 is clamped with the corresponding ratchet rack 47, the other end of each tilting rod 49 is connected with one end of a compression spring 50, the other end of each compression spring 50 is connected with a boss 51, each boss 51 is arranged at the lower end of each square hole 48, and each boss 51 is connected with the outer side of the corresponding support tube 46.
In one embodiment, door panels 34 are arranged on the left side plate 27 and the right side plate 27, the door panels 34 are hinged to the side plates 27 through hinges 35, magnetic buttons 36 are embedded in one corners of the door panels 34 far away from the hinges 35, suction points are correspondingly arranged on the left side plate 27 and the right side plate 27 and are connected with the door panels 34 in a suction mode, and door handles 37 are fixed on the outer sides of the door panels 34.
The working principle and the beneficial effects of the technical scheme are as follows: a user sets required temperature and humidity parameters for cell culture through the temperature and humidity adjusting device, and the culture box humidity display screen 22, the culture box temperature display screen 23 and the thermistor temperature display screen 24 are convenient for observing real-time temperature and humidity changes;
the incubator 26 can further reduce the influence of the outside on the incubator 1, the incubator 26 controls the temperature inside the incubator 26 through the heating fan, the wind shield 33 can disperse the hot wind of the heating fan 32, so that the temperature inside the incubator 26 is more uniform, when the support rod 45 is partially drawn out from the support tube 46, the ratchet rack 47 stirs one end of the tilting rod 49 away from the compression spring 50, so that the tilting rod 49 compresses the compression spring 50, when the tilting rod 49 slides to the next ratchet, the compression spring 50 rebounds, the tilting rod 49 returns to the state of being engaged with the ratchet rack 47, when the height of the incubator 26 needs to be reduced, the end of the tilting rod 49 connected with the compression spring 50 is pressed, and at this time, the support rod 45 can slide to the bottom of the support tube 46, by adjusting the height, the incubator 26 is better adapted to different microscopes, and the door panel 34 can be opened when needed. Compact structure can effectually prevent to cultivate the inhomogeneous problem of environmental temperature distribution, reduces that external conditions changes and arouses incubator 26 with cultivate the inside temperature fluctuation of box 1, reduces the pollution problem that external environmental conditions arouses. Through modular design, convenient and fast combined application is carried out aiming at different application scenes.
In one embodiment, the incubator 26 is provided with a light shielding shell 38, the light shielding shell 38 is composed of four left shell plates 39 and four right shell plates 40, two of the left shell plates 39 are fixed by screw fixing members 41, the joints of the three left shell plates 39 are clamped by diagonal sockets 42, two of the right shell plates 40 are fixed by the screw fixing members 41, the joints of the three right shell plates 40 are clamped by the diagonal sockets 42, and the joints of the left shell plates 39 and the right shell plates 40 are fixed by a plurality of magnets 43 in an absorbing manner.
In one embodiment, the culture cassette 1 is made of a photosensitive resin material.
In one embodiment, the temperature and humidity control box 7 is made of a resin material.
In one embodiment, the incubator 26 and the fixing beam 31 are both made of acrylic material.
The working principle and the beneficial effects of the technical scheme are as follows: the shading shell 38 can shade cells needing shading culture, four pieces of shading shell are firstly assembled respectively, the left shell plate 39 and four pieces of right shell plate 40 are sleeved on the incubator 26, the left shell plate 39 and the right shell plate 40 are fixed through magnet adsorption, and the left shell plate 39 and the right shell plate 40 are pulled open during disassembly, so that the assembly and the disassembly are convenient.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the utility model. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (9)
1. A cell culture system for a microscope, comprising: cultivate box (1), glass lid (2), activity bolt (3), temperature and humidity detector (4), mixed steam entry (5), aqua storage tank (6), it sets up to cultivate box (1) one side movable bolt (3), glass lid (2) can the block be in cultivate box (1) top, it sets up on the box (1) inner wall to cultivate temperature and humidity detector (4), mixed steam entry (5) set up cultivate on box (1), mixed steam entry (5) and the inside intercommunication of cultivating box (1), it is provided with aqua storage tank (6) to cultivate box (1) inner wall bottom a week, it settles on microscope objective table to cultivate box (1).
2. The cell culture system for a microscope according to claim 1, further comprising a temperature and humidity control system, the temperature and humidity control system comprising: the device comprises a temperature and humidity control box (7), a power switch (8), a first air inlet (9), a second air inlet (10), a mixed steam output interface (11), a first flowmeter (12), a second flowmeter (13), a steam heating bottle (14), a water changing port (15), a mixed steam output pipe (16), a mixed gas input pipe (17), a temperature and humidity detector interface (18), a thermistor (19) and a heating fan interface (20);
the power switch (8) is arranged on the temperature and humidity control box (7), the first air inlet (9) and the second air inlet (10) are arranged on a box body of the temperature and humidity control box (7), the first air inlet (9) is connected with the input end of the first flowmeter (12) through a pipeline, the second air inlet (10) is connected with the input end of the second flowmeter (13) through a pipeline, the output end of the first flowmeter (12) and the output end of the second flowmeter (13) are respectively communicated with one end of the mixed gas input pipe (17), the other end of the mixed gas input pipe (17) is communicated with the steam heating bottle (14), the steam heating bottle (14) is communicated with one end of the mixed steam output pipe (16), and the other end of the mixed steam output pipe (16) is communicated with the mixed steam output interface (11), the mixed steam output interface (11) is arranged on the temperature and humidity control box (7), the mixed steam output interface (11) is communicated with the mixed steam inlet (5) through a pipeline, the first flowmeter (12) and the second flowmeter (13) are arranged on the temperature and humidity control box (7), the steam heating bottle (14) is arranged in the temperature and humidity control box (7), the water changing port (15) is arranged at the top of the steam heating bottle (14), and the heating fan interface (20) is arranged below the temperature and humidity detector interface (18) of the culture box (1);
cultivate temperature and humidity detector interface (18) of box (1) with temperature and humidity detector (4) with thermistor (19) pass through the connecting wire and connect, thermistor (19) are installed cultivate box (1) top, thermistor (19)'s connecting wire middle part is fixed cultivate on the box (1) outer wall.
3. The cell culture system for the microscope according to claim 2, wherein the temperature and humidity control box (7) is provided with a temperature adjusting button (21), a culture box humidity display screen (22), a culture box temperature display screen (23), a thermistor temperature display screen (24) and a cooling fan (25), the temperature adjusting button (21) is electrically connected with the steam heating bottle (14), the heating fan interface (20) and the cooling fan (25), the culture box humidity display screen (22) and the culture box temperature display screen (23) are electrically connected with the temperature and humidity detector interface (18), and the thermistor temperature display screen (24) is electrically connected with the temperature and humidity detector interface (18).
4. The cell culture system for the microscope as claimed in claim 2, wherein the microscope body is built on an incubator (26), the microscope stage is installed inside the incubator (26), the incubator (26) is composed of a plurality of side plates (27), two top plates (28) and two bottom plates (29), adjacent side plates (27) are fixed through buckles (30), fixing beams (31) are arranged on the top and the bottom of the inside of the incubator (26) along the edges, the fixing beams (31) are fixedly connected with the side plates (27) and the bottom plates (29) through screws (44), a heating fan (32) is installed on one side of the incubator (26), the heating fan (32) is electrically connected with the heating fan interface (20), and a wind shield (33) is arranged at the air outlet of the heating fan (32).
5. The cell culture system for the microscope as claimed in claim 4, wherein door panels (34) are provided on the left and right side plates (27), the door panels (34) are hinged to the side plates (27) through hinges (35), magnetic buttons (36) are embedded in one corners of the door panels (34) far away from the hinges (35), the left and right side plates (27) are correspondingly provided with adsorption points which are connected with the door panels (34) in an adsorption manner, and door handles (37) are fixed on the outer sides of the door panels (34).
6. The cell culture system for the microscope as claimed in claim 4, wherein the incubator (26) is provided with a light shielding housing (38), the light shielding housing (38) is composed of four left shell plates (39) and four right shell plates (40), two of the left shell plates (39) are fixed by screw fasteners (41), the joints of the three left shell plates (39) are clamped by diagonal sockets (42), two of the right shell plates (40) are fixed by the screw fasteners (41), the joints of the three right shell plates (40) are clamped by the diagonal sockets (42), and the joints of the left shell plates (39) and the right shell plates (40) are fixed by a plurality of magnets (43).
7. A cell culture system for a microscope according to claim 1, wherein the culture cassette (1) is made of a photosensitive resin material.
8. A cell culture system for a microscope according to claim 2, wherein the temperature and humidity control box (7) is made of a resin material.
9. A cell culture system for a microscope according to claim 4, wherein the incubator (26) and the fixing beam (31) are both made of acrylic material.
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| CN202122714888.3U CN216274192U (en) | 2021-11-08 | 2021-11-08 | Cell culture system for microscope |
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| CN202122714888.3U CN216274192U (en) | 2021-11-08 | 2021-11-08 | Cell culture system for microscope |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
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2021
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
| CN116286315B (en) * | 2023-03-21 | 2023-10-27 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
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