CN212279333U - Animal hypoxemia/reoxygenation culture apparatus - Google Patents
Animal hypoxemia/reoxygenation culture apparatus Download PDFInfo
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- CN212279333U CN212279333U CN202021256541.8U CN202021256541U CN212279333U CN 212279333 U CN212279333 U CN 212279333U CN 202021256541 U CN202021256541 U CN 202021256541U CN 212279333 U CN212279333 U CN 212279333U
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Abstract
The utility model belongs to the field of medical test equipment, and relates to an animal hypoxia/reoxygenation culture device.A culture box is divided into a plurality of culture cavities by partition plates, each culture cavity is respectively provided with a culture drawer which can be opened and closed independently, the upper surface of the culture box is provided with a transparent cover plate, and a feeding kettle is arranged on the transparent cover plate; the connection controller comprises an oxygen concentration detection conduit and branch air inlet conduits, wherein each oxygen concentration detection conduit and each branch air inlet conduit are communicated with each culture pumping box and the control box; the control box is provided with an oxygen concentration sensor and an oxygen concentration controller, and the animal hypoxia/reoxygenation culture device can simultaneously meet the requirements of the independence, automatic control, long-time culture and the realization of two functions of hypoxia/reoxygenation of animal culture.
Description
Technical Field
The utility model belongs to medical test equipment field relates to an animal hypoxemia reoxygenation culture apparatus, especially relates to one kind and can effectively adjust oxygen concentration in the incubator, creates the required hypoxemia reoxygenation of animal model and cultivates the environment to furthest's assurance does not disturb the animal hypoxemia reoxygenation culture apparatus that the control cultivates the environment.
Background
Changes in oxygen content are one of the most important regulators throughout the life cycle, extending from embryonic development, maintenance of normal body function, to pathological processes such as disease and aging, with hypoxia being one of the most common basic environments. Hypoxic culture is a culture state when oxygen in cells or organs is lower than that in air, and plays an important role in human medical research. In the prior art, an experimental animal model needs to be established by simulating a hypoxia environment, and the experimental animal model is used for researching a series of pathological, physiological and molecular biological index changes of animals or cells under a relatively hypoxia environment. The common hypoxia incubator is a hypoxia state formed by premixing air and nitrogen according to a certain proportion, is generally simple in design, and generally has the following problems:
1. traditional animal incubator can only realize the establishment of hypoxemia environment, and the function is single, and traditional animal is cultivateed the experiment and not only can use the hypoxemia and cultivates the environment, can use reoxygenation environment more times moreover, because traditional experimental apparatus's function is single, when the reoxygenation environment is being found, often oxygen concentration recovery rate is slow, and long-time recovery process can influence the precision of experiment model moreover.
2. The traditional animal incubator has the defect of centralized culture, the animals of a control group and an experimental group are cultured together, relatively independent culture environments are lacked, some animals need to be treated in the culture process, but the animals are cultured together, so that the culture environments of other groups are inevitably influenced when the animals are treated, the nonsterile animal model construction process is caused, and the experimental result is finally influenced.
3. Traditional animal incubator lacks relative independence and long-term system of raising for, when needs put in food and water for the animal, need open the incubator, put in a certain amount of water and food toward the incubator, inevitable meeting brought the influence to the culture environment after opening the incubator, need readjust oxygen concentration, not only waste time and energy, process time is long moreover, be difficult to guarantee whole cultivation in-process, the continuity and the stability of animal culture environment, finally influence the experimental result.
4. Traditional animal incubator lacks automatic closed-loop control, and the regulation of oxygen concentration needs artificial manual intervention many times to adjust and control the inlet flow of oxygen repeatedly for a long time, whole accommodation process not only loaded down with trivial details and accuracy is low, causes the experimental efficiency low, delays the experiment progress, extravagant experiment air supply.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model discloses a solve the function singleness that above-mentioned traditional animal incubator exists, be not convenient for to the accurate automatic handling of every animal and control, influence the problem of experimental result precision, provide an animal hypoxemia/reoxygenation culture apparatus, this animal hypoxemia/reoxygenation culture apparatus can satisfy the independence that the animal was cultivateed, automatic control, the realization of two kinds of functions of long-time cultivation and hypoxemia/reoxygenation simultaneously.
In order to achieve the aim, the utility model provides an animal hypoxia/reoxygenation culture device, which comprises a culture box, a control box and a connection controller;
wherein the incubator is provided with two or more than two clapboards which are the same with the end surfaces of the incubator and are divided into a plurality of culture cavities by the clapboards, each culture cavity is respectively provided with an independently opened and closed culture drawer, the upper surface of the incubator is provided with a transparent cover plate, and the transparent cover plate is provided with a feeding kettle for supplying nutrients to animals in the culture drawer;
the connection controller comprises an oxygen concentration detection conduit and branch air inlet conduits, wherein each oxygen concentration detection conduit and each branch air inlet conduit are communicated with each culture pumping box and the control box;
the control box is provided with an oxygen concentration sensor for monitoring the oxygen concentration in each culture extraction box in real time and an oxygen concentration controller for controlling the opening degree of the upper air inlet control valve of the branch air inlet pipe.
The beneficial effect of this basic scheme lies in: each culture cavity which is uniformly separated by the partition board in the incubator is used for respectively culturing a single group of animals, the same living place is provided for the animals, and the culture environment of other animals is not influenced when the single animal is treated. The drawing type culture drawing box is convenient for taking animals. The transparent cover plate on the incubator is convenient for an experiment operator to observe and record the culture process of the animal, and the feeding kettle on the transparent cover plate is used for providing necessary water and nutrient substances for culturing the animal in the extraction box, so that the long-term culture of the animal is realized. The oxygen concentration detection conduit is used for realizing the real-time adjustment of the oxygen concentration in each incubator. Each culture pumping box is communicated with the main air inlet duct through a three-way connecting pipe on a branch air inlet pipe, and different media (O) are introduced into the main air inlet duct2Or N2) And the fast switching of the low-oxygen/reoxygenation culture conditions in each culture drawer is realized. The oxygen concentration controller signal connection on air control valve and the control box on every branch intake pipe, the incubator passes through oxygen concentration detection pipe intercommunication with the control box and forms the closed loop, oxygen concentration sensor through the control box real time monitoring every cultivates and takes out the oxygen concentration in the box, oxygen concentration sensor sends control signal to oxygen concentration controller according to the oxygen concentration requirement of setting for, and then the air control valve aperture on the every branch intake pipe of control, the adjustment gets into the gas volume in every cultivation and takes out the box, realize the accurate automatic control of every cultivation and take out the box interior oxygen concentration.
Further, the transparent cover plate, the casing of incubator and the cultivation are taken out the box upper surface and are seted up the first pipe connecting hole that link up, oxygen concentration detection pipe one end is passed first pipe connecting hole and is taken out the box intercommunication with every cultivation, the second pipe connecting hole has been seted up to the casing left side lateral wall lower part of incubator, the cultivation is taken out and is provided with the cultivation that link up with the second pipe connecting hole on the box and takes out the box inlet port, branch's inlet duct one end is passed second pipe connecting hole and is taken out the box intercommunication with every cultivation, the casing of incubator and the cultivation are taken out the right side lateral wall upper portion of box and are seted.
Further, an oxygen concentration display screen for displaying the oxygen concentration in the control box is arranged on the control box. Has the advantages that: the oxygen concentration display screen is used for displaying the oxygen concentration in the control box and can set and control the oxygen concentration controller.
Further, the contact surface of drawing box and incubator is provided with silica gel adhesive layer in the cultivation. Has the advantages that: the silica gel adhesive layer of the contact surface of the culture box and the incubator can ensure that the culture box has airtightness after being closed, and air leakage or animal escape is prevented.
Furthermore, the end face of the incubator is in a right-angled trapezoid shape. Has the advantages that: the end face of the incubator is in a right-angled ladder shape, and the transparent cover plate on the upper surface of the incubator is in a slope shape, so that the feeding kettle can supply water and nutrition for animals in each culture drawer.
Furthermore, each cultivation drawer is internally provided with a food placing area for placing food. Has the advantages that: the food placing area in the culture drawer box is used for placing food for the survival of animals.
Furthermore, the transparent cover plate and the top of the culture pumping box are provided with through small holes, and the water outlet end of the feeding kettle is fixedly provided with a water delivery conduit with a free end penetrating through the small holes.
Furthermore, a one-way exhaust valve is fixedly arranged on the exhaust hole. Has the advantages that: the exhaust hole with the one-way exhaust valve is convenient for discharging the stink generated by the animals in the culture pumping box.
The beneficial effects of the utility model reside in that:
1. the utility model discloses an animal hypoxemia/reoxygenation culture apparatus separates the incubator for a plurality of cultivation cavitys through the baffle to cultivate the animal of different groups in independent space, both conveniently handled each experimental animals of group at any time, guaranteed again when handling, do not influence the animal culture environment of other groups, realize independent cultivation. In the aspect of oxygen concentration control, the oxygen concentration sensor sends a control signal to the oxygen concentration controller according to a set oxygen concentration requirement, so that the opening degree of an air inlet control valve on each branch air inlet pipe is controlled, the amount of gas entering each cultivation pumping box is adjusted, and accurate automatic control of the oxygen concentration in each cultivation pumping box is realized. By introducing different media (O) into the main inlet conduit2Or N2) And the fast switching of the low-oxygen/reoxygenation culture conditions in each culture drawer is realized. In addition, in order to ensure the continuous culture for a relatively long time, the feeding system is designed, so that the long-term culture can be realized under the condition that the incubator is not opened, and the culture environment of other incubators is not influenced even if the incubator is opened.
2. The utility model discloses an animal hypoxemia/reoxygenation culture apparatus for the hypoxia incubator of traditional manual regulation, has realized the accurate closed loop automatic control of oxygen concentration in the incubator, has improved the regulation efficiency of oxygen concentration greatly. And can realize two functions of quick hypoxia/reoxygenation in the same device according to different introduced gas media. The design of the independent culture drawing box is adopted, so that the animals in the drawing box can be conveniently treated at any time, and the culture of other animals in the drawing box is ensured not to be interfered. The kettle and the food placing area arranged by the device can guarantee the long-term continuity of animal culture to the maximum extent, and avoid the interference of experimental conditions caused by the feeding of necessary food. The animal hypoxia/reoxygenation culture device can simultaneously meet the requirements of independence, automatic control, long-time culture and realization of two functions of hypoxia/reoxygenation of animal culture.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and/or combinations particularly pointed out in the appended claims.
Drawings
For the purposes of promoting a better understanding of the objects, features and advantages of the invention, reference will now be made to the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic structural view of the animal hypoxia/reoxygenation culture apparatus of the present invention;
FIG. 2 is a schematic view of the structure of the incubator of the animal hypoxia/reoxygenation culture apparatus of the present invention;
FIG. 3 is a schematic view of the structure of the baffle plate installed in the incubator of the animal hypoxia/reoxygenation culture apparatus of the present invention;
FIG. 4 is a schematic structural view of a culture drawer in the low-oxygen/reoxygenation culture device for animals of the present invention;
FIG. 5 is a schematic view of the structure of the transparent cover plate in the low oxygen/reoxygenation culture apparatus for animals of the present invention;
FIG. 6 is a schematic structural view of a partition plate in the low oxygen/reoxygenation culture apparatus for animals of the present invention;
fig. 7 is a schematic view of the shell structure of the incubator of the animal hypoxia/reoxygenation culture apparatus of the present invention.
Reference numerals: the oxygen concentration controller comprises an oxygen concentration controller 1, a control box 2, an oxygen concentration sensor 3, an oxygen concentration display screen 4, an oxygen concentration detection conduit 5, a feeding kettle 6, a first conduit connecting hole 7, an incubator 8, a second conduit connecting hole 9, an air inlet control valve 10, a branch air inlet conduit 11, a main air inlet conduit 12, a three-way connecting pipe 13, a lead 14, a conduit 15, an exhaust hole 16, a culture extraction box 17, a partition plate 18, a transparent cover plate 19, a culture extraction box air inlet hole 20, a silica gel adhesive layer 21 and a food placing area 22.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The present invention can also be implemented or applied through other different specific embodiments, and various details in the present specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the drawings provided in the following embodiments are only for illustrating the basic idea of the present invention, and the features in the following embodiments and examples may be combined with each other without conflict.
Wherein the showings are for the purpose of illustrating the invention only and not for the purpose of limiting the same, and in which there is shown by way of illustration only and not in any way limiting the scope of the invention; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are terms such as "upper", "lower", "left", "right", "front", "back", etc., indicating directions or positional relationships based on the directions or positional relationships shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore, the terms describing the positional relationships in the drawings are only used for illustrative purposes and are not to be construed as limiting the present invention, and those skilled in the art can understand the specific meanings of the terms according to specific situations.
The animal hypoxia/reoxygenation culture device shown in figures 1-7 is applied to the simulation of animal hypoxia/reoxygenation survival conditions. For example, in animal experiments, hypoxia/reoxygenation conditions are often involved, the oxygen concentration needs to be effectively adjusted, a hypoxia/reoxygenation culture environment required by an animal model is created, the culture environment of a control group is not interfered to the maximum extent, and the establishment of the hypoxia/reoxygenation culture conditions of the animals is realized.
The animal hypoxia/reoxygenation culture device mainly comprises an incubator 8, a control box 2 and a connection controller communicated with the incubator and the control box. Wherein the terminal surface of incubator 8 becomes right trapezoid shape, the incubator is separated four the same cultivation cavitys by three the same and 18 with the same baffle of incubator terminal surface, it can open independently and close to cultivate in every cultivation cavity and take out box 17 to dispose one respectively, it is similar to the drawer to cultivate to take out the box, a place of action for providing singly organizing animal cultivation, every is cultivateed and is taken out and be provided with the food that supplies the food to place and place district 22, in order to guarantee the persistence that the animal was cultivateed, and cultivate to take out the box 17 and be provided with silica gel laminating 21 with the sealed interface of incubator, it has the seal after closing to ensure to cultivate to take out the box, prevent gas leakage or animal escape.
The upper surface of the shell of the incubator 8 is provided with a transparent cover plate 19 with a certain thickness, which is convenient for observing and recording the animal culture process, and the transparent cover plate is fixed on the top of each culture cavity in a slope shape. The transparent cover plate is provided with a feeding kettle 6, and the water outlet end of the feeding kettle 6 passes through a small hole penetrating through the transparent cover plate 19 and the culture drawer through a water delivery conduit to provide necessary water and nutrition for the animals in the incubator.
The connection controller comprises an oxygen concentration detection conduit 5 which is connected to a first conduit connecting hole and communicated with the control box, a branch air inlet conduit 11 and a main air inlet conduit 12 which are connected to a second conduit connecting hole, the oxygen concentration detection conduit realizes real-time adjustment of the oxygen concentration in the incubator, the main air inlet conduit is connected with each branch air inlet pipe 11 through a three-way connecting pipe 13, an air inlet control valve 10 is arranged on each branch air inlet pipe 11, the air inlet control valve 10 is in signal connection with the control box through a lead 14 with a lead pipe 15, and the air inlet control valve 10 is used for controlling the flow of oxygen entering each culture pumping box.
The control box is provided with an oxygen concentration sensor 3 for monitoring the oxygen concentration in each culture extraction box in real time, an oxygen concentration controller 1 for controlling the opening degree of an upper air inlet control valve of the branch air inlet pipe and an oxygen concentration display screen 4 for displaying the oxygen concentration in the control box. Oxygen concentration sensor 3 realizes the real time monitoring to oxygen concentration in the incubator, and oxygen concentration controller 1 passes through the control command that oxygen concentration controller sent through the wire in the wire pipe according to the oxygen concentration requirement of setting for, adjusts the aperture of the last air control valve in every branch intake pipe, realizes drawing box interior oxygen concentration's regulation and accurate control to every cultivation. The oxygen concentration display 4 may also set and operate an oxygen concentration controller.
When the animal hypoxia/reoxygenation culture device is used, small animals to be tested are respectively placed in each culture drawer 17, and the culture drawers 17 are inserted into culture cavities separated by partition plates. The animal in taking out the box is supplied water and nutrient substance and is placed the district at food and be used for placing the food that the animal survived for the cultivation through for the kettle, set for and control the oxygen concentration controller through oxygen concentration display screen 4, and draw box 17 interior letting in oxygen through main admission conduit 12 to every cultivation, oxygen concentration detection pipe 5 transmits the incubator interior oxygen concentration to the control box, oxygen concentration sensor 3 shows the control box interior oxygen concentration, oxygen concentration controller 1 expects according to the oxygen concentration who sets for and oxygen concentration sensor 3 detects, the control instruction that wire transmission oxygen concentration controller sent through the wire in the wire pipe, adjust the aperture of air control valve 10 on every branch intake pipe 11, realize drawing box interior oxygen concentration's regulation and accurate control to every cultivation.
It is also possible to introduce different media (O) into the main intake conduit2Or N2) And the fast switching of the low-oxygen/reoxygenation culture conditions in each culture drawer is realized.
Finally, the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the scope of the claims of the present invention.
Claims (8)
1. An animal hypoxia/reoxygenation culture device is characterized by comprising a culture box, a control box and a connection controller;
wherein the incubator is provided with two or more than two clapboards which are the same with the end surfaces of the incubator and are divided into a plurality of culture cavities by the clapboards, each culture cavity is respectively provided with an independently opened and closed culture drawer, the upper surface of the incubator is provided with a transparent cover plate, and the transparent cover plate is provided with a feeding kettle for supplying nutrients to animals in the culture drawer;
the connection controller comprises an oxygen concentration detection conduit and branch air inlet conduits, wherein each oxygen concentration detection conduit and each branch air inlet conduit are communicated with each culture pumping box and the control box;
the control box is provided with an oxygen concentration sensor for monitoring the oxygen concentration in each culture extraction box in real time and an oxygen concentration controller for controlling the opening degree of the upper air inlet control valve of the branch air inlet pipe.
2. The animal hypoxia/reoxygenation culture device according to claim 1, wherein the transparent cover plate, the housing of the incubator and the upper surface of the culture drawer are provided with a first conduit connection hole therethrough, one end of the oxygen concentration detection conduit passes through the first conduit connection hole and communicates with each culture drawer, the lower portion of the left side wall of the housing of the incubator is provided with a second conduit connection hole, the culture drawer is provided with an air inlet hole therethrough, the air inlet hole is communicated with the second conduit connection hole, one end of the branch air inlet conduit passes through the second conduit connection hole and communicates with each culture drawer, and the housing of the incubator and the upper portion of the right side wall of the culture drawer are provided with a through air outlet hole.
3. The animal hypoxia/reoxygenation culture apparatus according to claim 1, wherein the control box is provided with an oxygen concentration display screen for displaying the oxygen concentration in the control box.
4. The animal hypoxia/reoxygenation culture device of claim 1, wherein the contact surface of the culture drawer and the incubator is provided with a silica gel adhesive layer.
5. The animal hypoxia/reoxygenation culture apparatus of claim 1, wherein the end face of the culture box is rectangular trapezoid.
6. The animal hypoxia/reoxygenation culture device of claim 1, wherein each of the plurality of culture drawers is provided with a food placement area for placing food therein.
7. The animal hypoxia/reoxygenation culture device of claim 1, wherein the transparent cover plate and the top of the culture drawer are provided with through holes, and the water outlet end of the feeding kettle is fixedly provided with a water delivery conduit with a free end passing through the holes.
8. The animal hypoxia/reoxygenation culture device of claim 2, wherein a one-way exhaust valve is fixedly mounted on the exhaust hole.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021256541.8U CN212279333U (en) | 2020-07-01 | 2020-07-01 | Animal hypoxemia/reoxygenation culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021256541.8U CN212279333U (en) | 2020-07-01 | 2020-07-01 | Animal hypoxemia/reoxygenation culture apparatus |
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| CN212279333U true CN212279333U (en) | 2021-01-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111685051A (en) * | 2020-07-01 | 2020-09-22 | 重庆医科大学 | Animal hypoxemia/reoxygenation culture apparatus |
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2020
- 2020-07-01 CN CN202021256541.8U patent/CN212279333U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111685051A (en) * | 2020-07-01 | 2020-09-22 | 重庆医科大学 | Animal hypoxemia/reoxygenation culture apparatus |
| CN111685051B (en) * | 2020-07-01 | 2024-06-04 | 重庆医科大学 | Animal hypoxia/reoxygenation culture device |
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