CN216018765U

CN216018765U - Program cooling equipment

Info

Publication number: CN216018765U
Application number: CN202121237666.0U
Authority: CN
Inventors: 倪孝杰; 赖雪聪; 徐铭恩
Original assignee: Regenovo Biotechnology Co ltd
Current assignee: Regenovo Biotechnology Co ltd
Priority date: 2021-06-03
Filing date: 2021-06-03
Publication date: 2022-03-15
Anticipated expiration: 2031-06-03

Abstract

A program cooling device comprises a program cooling mechanism, an ultralow temperature storage mechanism, a transfer mechanism, an environment control mechanism and a shell; the program cooling mechanism comprises a fixed support and a circulating mechanism, wherein the fixed support is provided with at least two constant-temperature freezing blocks with different temperatures and two temperature conducting blocks; the driving circulation mechanism, the temperature conduction block and the circulation mechanism synchronously and circularly move; the program cooling mechanism, the ultralow temperature storage mechanism and the transfer mechanism are arranged in the shell, and the environment control mechanism is embedded in the top of the shell; through set up the freezing piece of the constant temperature that two at least temperatures are different on the fixed bolster and the circulation mechanism fixedly be provided with the freezing piece of leading the temperature piece the same in quantity of constant temperature, lead and be formed with the cooling well that is used for placing the freezing pipe in the temperature piece, also be convenient for add new freezing pipe when realizing carrying out the ladder cooling to freezing pipe in the cooling well, the process is simple, and easy operation has better suitability.

Description

Program cooling equipment

Technical Field

The utility model relates to the technical field of cell freezing, in particular to program cooling equipment.

Background

The cell freezing is to store the cells in a low-temperature environment, so that the metabolism of the cells is basically suspended, and thus, the completeness of various biological characteristics of the cells after long-term storage is ensured for subsequent use. The cell freezing process is a necessary step for freezing and storing, the quality of the freezing effect directly influences the biological activity and biological characteristics of the frozen and stored cells, and the cell freezing process plays a very important role in long-term storage of the cells.

At present, the conventional programmed cooling method for freezing and storing cells has two types: a program cooling instrument and a program cooling box. When the programmed cooling instrument is adopted, the sample placed in the refrigerating box is cooled to the required temperature according to a cooling curve set by a program; when the program cooling box is adopted, a normal-temperature sample needs to be placed in the program cooling box according to the specification, then the program cooling box is placed in a refrigerator at the required temperature, and the program cooling box can ensure that the sample inside the program cooling box is cooled to the required temperature at a specific speed. However, both of the two program cooling methods have the defect that the normal-temperature sample cannot be added in the program cooling process, the requirement of a user for adding a new sample in real time cannot be met, the use experience of the user is affected, and the method is not suitable for continuous work.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide program cooling equipment which mainly comprises a program cooling mechanism and an ultralow temperature storage mechanism, has a simple structure, is convenient for a user to add a new normal-temperature sample in the program cooling process, and has better applicability.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the program cooling mechanism comprises:

fixing a bracket;

the constant-temperature freezing blocks are arranged at intervals and fixedly arranged on the same side of the fixed support, and a refrigerating surface is formed on the same side of each constant-temperature freezing block;

the refrigerating device comprises at least two temperature conducting blocks, a refrigerating chamber and a refrigerating chamber, wherein each temperature conducting block is provided with a cooling well for accommodating a freezing pipe, and any one temperature conducting block can be attached to any one refrigerating surface and can move relatively;

the circulating mechanism is fixedly arranged on the fixed support, and each temperature conduction block is fixedly connected to the circulating mechanism; and driving the circulating mechanism, and enabling the temperature guide block and the circulating mechanism to synchronously perform circulating motion so as to enable the temperature guide block to move relative to the constant-temperature freezing block.

Preferably, the outer side surface of each constant-temperature freezing block is fixedly sleeved with a first heat preservation layer, and the ring side of the heat conduction block is fixedly sleeved with a second heat preservation layer.

Preferably, the distribution mode and the number of the constant-temperature freezing blocks can be set according to actual needs, for example, the constant-temperature freezing blocks are circularly distributed, the number of the constant-temperature freezing blocks is 8, and the temperature of each constant-temperature freezing block is decreased gradually in the clockwise direction or the counterclockwise direction; or in a chain distribution manner, which is not limited herein.

Preferably, the circulating mechanism at least comprises a rotating disc, and each temperature conducting block is arranged in a circular shape and fixedly arranged on the rotating disc.

The ultra-low temperature storage mechanism includes:

a storage rack formed with an accommodation space for storing the freezing pipes;

the storage rack is connected with the lifting mechanism;

the lifting mechanism is arranged in the freezing bin and can drive the storage rack to enter or move out of the freezing bin;

and the refrigerating piece is fixedly arranged on the outer surface of the refrigerating bin.

The program cooling equipment also comprises a transfer mechanism and an environment control mechanism; the transfer mechanism is used for transferring the cryopreserved sample; the environmental control mechanism includes: the temperature and humidity controller comprises a regulation and control box, a primary filter, a centrifugal fan, a high-efficiency filter, a sensor assembly, a temperature and humidity controller and a sterilization actuator, and is used for keeping the environment state required by the program cooling equipment during operation.

The program cooling mechanism, the ultralow temperature storage mechanism and the transfer mechanism are arranged in the shell, and the environment control mechanism is embedded in the top of the shell.

Compared with the prior art, the utility model has the beneficial effects that:

the program cooling equipment that provides among the above-mentioned technical scheme, it freezes the piece through the fixed constant temperature that sets up two at least temperature differences on the fixed bolster, it freezes the piece the same with constant temperature to be fixed with on the circulation mechanism on the fixed bolster to set up leads the temperature piece, it is formed with the cooling well that is used for placing the cryopreserved pipe to lead in the temperature piece, when circulation mechanism does the cyclic motion, lead temperature piece and circulation mechanism simultaneous movement, make arbitrary one lead the temperature piece homoenergetic and remove to the refrigeration face of another adjacent constant temperature refrigeration piece by the refrigeration face of a constant temperature refrigeration piece, so set up, also be convenient for add new cryopreserved pipe when can be convenient for realize carrying out the ladder cooling to the cryopreserved pipe in the cooling well, the process is simple, easy operation has better suitability.

Drawings

Fig. 1 is a schematic structural diagram of a process cooling device according to an embodiment of the present invention.

Fig. 2 is a partial structural schematic diagram of the temperature programming mechanism shown in fig. 1.

Fig. 3 is a schematic structural diagram of the temperature reduction program mechanism shown in fig. 2 from another perspective.

Fig. 4 is a schematic structural view of the ultra-low-temperature storage mechanism shown in fig. 1.

100. A programmed cooling mechanism; 10. fixing a bracket; 20. freezing the block at constant temperature; 30. a temperature conducting block; 31. a cooling well; 40. freezing and storing the tube; 50. a circulating mechanism; 60. a first insulating layer; 70. a second insulating layer;

200. a housing; 210. a support frame;

300. an ultra-low temperature storage mechanism; 310. a storage rack; 320. a lifting mechanism; 330. a freezing bin; 340. a refrigeration member;

400. transfer mechanism

500. Environmental control mechanism

Detailed Description

The present invention will now be described in more detail with reference to the accompanying drawings, in which the description of the utility model is given by way of illustration and not of limitation. The various embodiments may be combined with each other to form other embodiments not shown in the following description.

Referring to fig. 1 to 4, in an embodiment of the present invention, a novel temperature programming device is provided, which has a simple structure, can implement temperature programming on a normal temperature sample, and is convenient for adding a new normal temperature sample in real time, and has better applicability.

The temperature programming device at least comprises a shell 200, a temperature programming mechanism 100, an ultra-low temperature storage mechanism 300, a transfer mechanism 400 and an environment control mechanism 500. The temperature programming mechanism 100 is fixed on the inner side wall of the casing 200, the ultra-low temperature storage mechanism 300 and the transfer mechanism 400 are both fixed on the support frame 210 inside the casing 200, and the environment control mechanism 500 is arranged above the casing 200.

As shown in fig. 2, the temperature programming mechanism 100 at least comprises: the fixed bracket 10, the fixed bracket 10 is fixed on the inside wall of the body 200; the refrigerator comprises at least two constant-temperature freezing blocks 20 with different temperatures, wherein the constant-temperature freezing blocks 20 are arranged at intervals, each constant-temperature freezing block 20 is fixed on the same side surface of a fixed support 10, and a refrigerating surface is formed on each constant-temperature freezing block 20; at least two lead the temperature piece 30, each leads the temperature piece 30 and all is formed with the cooling well 31 that is used for the holding to freeze and deposits pipe 40, freezes and deposits and be used for placing the cell suspension in the pipe 40, and arbitrary one leads the temperature piece 30 homoenergetic and can take place relative movement with arbitrary one refrigeration face to the laminating, promptly: any one of the temperature conducting blocks 30 can move from the refrigerating surface of one of the constant-temperature refrigerating blocks 20 to the refrigerating surface of the other constant-temperature refrigerating block 20, so that the temperature conducting block 30 can obtain energy from the refrigerating surface to achieve the cooling effect; and the circulating mechanism 50, the circulating mechanism 50 is fixedly arranged on the fixed support 10, each temperature conduction block 30 is fixedly connected to the circulating mechanism 50, the circulating mechanism 50 can rotate relative to the constant-temperature freezing block 20, when the circulating mechanism 50 is driven, the temperature conduction blocks 30 and the circulating mechanism 50 synchronously perform circulating motion, one temperature conduction block 30 can move to the refrigerating surface of the adjacent constant-temperature freezing block 20 from the refrigerating surface of one constant-temperature freezing block 20, and the arrangement is adopted, so that the step refrigeration can be realized, and meanwhile, a new freezing pipe 40 can be conveniently added.

Preferably, in order to improve the temperature stability, a first heat insulation layer 60 is fixedly sleeved on the outer side surface of each constant temperature freezing block 20, and a second heat insulation layer 70 is fixedly sleeved on the ring side of each heat conduction block 30; the first insulating layer 60 and the second insulating layer 70 are both made of heat insulating materials and are used for realizing the heat insulating function.

Preferably, the quantity of the constant temperature freezing block 20 is 8, the constant temperature freezing block 20 is a refrigerating device, the temperature of each constant temperature freezing block 20 is different, each constant temperature freezing block 20 is circularly distributed, and each constant temperature freezing block 20 is fixedly arranged on the same side between the two blocks. It should be noted that the temperature of each constant temperature freezing block 20 decreases sequentially in a clockwise or counterclockwise direction, that is: the temperature of the adjacent constant temperature freezing blocks 20 is sequentially decreased from the normal temperature to a desired temperature.

Preferably, the circulating mechanism 50 at least comprises a rotating disk and an electric driving element, the rotating disk is fixedly connected to the fixed bracket 10, and the electric driving element is arranged on the rotating disk and is used for driving the rotating disk to rotate in a clockwise direction or a counterclockwise direction; meanwhile, the number of the temperature guide blocks 30 is the same as that of the constant-temperature freezing blocks 20, preferably 8, each temperature guide block 30 is fixed on the rotating disc, and each temperature guide block 30 can be attached to the refrigerating surface of the corresponding constant-temperature freezing block 20.

It can be understood that, taking the example of cooling the cell suspension in the freezing tube 40 to-80 ℃ at a specific rate, 8 constant temperature freezing blocks 20 are sequentially arranged along the circumferential direction of the rotating disk, and the temperature of each constant temperature freezing block 20 is sequentially decreased progressively along the homeotropic direction and respectively maintained at 0 ℃, -10 ℃, -20 ℃, -30 ℃, -40 ℃, -50 ℃, -60 ℃, -70 ℃ and-80 ℃, correspondingly, 8 temperature guide blocks 30 are fixed on the rotating disk, each constant temperature freezing block 20 is correspondingly attached to any one of the temperature guide blocks 30, and each temperature guide block 30 can be driven by the rotating disk to move to the position above the adjacent constant temperature freezing block 20, so as to achieve the purpose of stepped cooling.

It is understood that the operation of the above-described programmable cooling mechanism 100 can be described as follows: the technical personnel place the freezing tube 40 filled with the cell suspension in the cooling well 31 above the constant temperature freezing block 20 at 0 ℃, start the rotating disc to rotate when the temperature of the freezing tube 40 is reduced to 0 ℃ so as to move the temperature guide block 30 provided with the freezing tube 40 to the upper part of the adjacent constant temperature freezing block 20 at minus 10 ℃, move the temperature guide block 30 provided with the freezing tube 40 to the upper part of the adjacent constant temperature freezing block 20 at minus 20 ℃ after the temperature of the freezing tube 40 is reduced to minus 10 ℃, and analogize in turn until the temperature of the freezing tube 40 is reduced to the required temperature of minus 80 ℃, and take out the freezing tube 40. Meanwhile, when the temperature guide block 30 provided with the freezing tube 40 is moved to the upper part of the adjacent constant-temperature freezing block 20, one temperature guide block 30 is positioned above the constant-temperature freezing block 20 at 0 ℃, technicians can additionally add the freezing tube 40 into the cooling well 31 positioned above the constant-temperature freezing block 20 at 0 ℃ according to needs, and the programmed cooling mechanism 100 can perform gradient cooling on a plurality of freezing tubes 40 filled with cell suspension at the same time.

It is understood that the number of the constant temperature freezing blocks 20 may be any number, and the temperature gradient thereof may be arranged as desired; meanwhile, the rotation mode of the freezing storage pipe 40 is various and is not limited to a rotating disc type, and other modes can be adopted.

In one embodiment, the programmed cooling device can achieve stepped cooling by the programmed cooling mechanism 100, and can achieve cryopreservation at-196 ℃ of the cell suspension in the cryopreservation tube 40 by the ultralow temperature storage mechanism 300.

Specifically, as shown in fig. 1 and 4, the ultra-low-temperature storage mechanism 300 includes at least: the storage rack 310 is internally provided with an accommodating space for storing the freezing storage pipe 40; the lifting mechanism 320 is connected with the storage rack 310, so that the lifting mechanism 320 can drive the storage rack 310 to move; the lifting mechanism 320 is arranged on the freezing chamber 330, and the storage rack 310 can be moved into or out of the freezing chamber 330 in the process of ascending or descending the lifting mechanism 320; meanwhile, the refrigerating member 340 is fixedly arranged on the outer surface of the freezing chamber 330, and can play a role in refrigerating the freezing chamber 330, so that the internal temperature of the freezing chamber 330 is always maintained at-196 ℃, and the freezing pipe 40 at-80 ℃ can be cooled to-196 ℃. Preferably, the lifting mechanism 320 may be any moving component capable of being used for position transfer, such as a pneumatic cylinder, a hydraulic cylinder, an electric push rod, a transmission guide rail, and the like; the working principle of the refrigerating element 340 includes, but is not limited to, cooling by a semiconductor refrigerating plate, slow evaporation and heat absorption of liquid nitrogen, heat exchange refrigeration by a heat engine, and the like.

In one embodiment, the process cooling device further comprises a transfer mechanism 400 and an environmental control mechanism 500; the transfer mechanism 400 is used for transferring the cryopreserved sample; the environmental control mechanism 500 includes: the temperature and humidity controller comprises a regulation and control box, a primary filter, a centrifugal fan, a high-efficiency filter, a sensor assembly, a temperature and humidity controller and a sterilization actuator, and is used for keeping the environment state required by the program cooling equipment during operation.

The programmed cooling device provided in the above embodiment implements the stepwise cooling from the normal temperature to-80 ℃ for the cell suspension in the cryopreservation tube 40 by the programmed cooling mechanism 100, and implements the cooling from-80 ℃ to-196 ℃ by the ultra-low temperature storage mechanism 300; in addition, in the in-process of procedure cooling equipment operation, the transportation process of various consumptive materials and materials is realized to accessible transport mechanism 400 to keep the required environmental state in operation via environmental control mechanism 500, so set up, can realize waiting to freeze the lasting input of depositing biological agent and the lasting output of procedure cooling biological agent, provide the flexibility of procedure cooling on the one hand, on the other hand is applicable to the cooperation and the management of automation equipment more.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. The program cooling equipment is characterized by comprising a program cooling mechanism (100), an ultralow-temperature storage mechanism (300), a transfer mechanism (400), an environment control mechanism (500) and a shell (200); the program cooling mechanism (100) comprises:

a fixed bracket (10);

the refrigerator comprises at least two constant-temperature freezing blocks (20) with different temperatures, wherein the constant-temperature freezing blocks (20) are arranged at intervals and fixedly arranged on the same side of a fixed support (10), and a refrigerating surface is formed on the same side of each constant-temperature freezing block (20);

the refrigerating system comprises at least two temperature conducting blocks (30), wherein each temperature conducting block (30) is provided with a cooling well (31) for accommodating a freezing storage pipe (40), and any one temperature conducting block (30) can be attached to any one refrigerating surface and can move relatively;

the circulating mechanism (50) is fixedly arranged on the fixed support (10), and each temperature conduction block (30) is fixedly connected to the circulating mechanism (50); the circulating mechanism (50) is driven, and the temperature guide block (30) and the circulating mechanism (50) synchronously perform circulating motion so as to enable the temperature guide block (30) to move relative to the constant-temperature freezing block (20);

the program cooling mechanism (100), the ultra-low temperature storage mechanism (300) and the transfer mechanism (400) are installed in the shell (200), and the environment control mechanism (500) is installed at the top of the shell (200) in an embedded mode.

2. The program cooling device according to claim 1, wherein a first heat insulation layer (60) is fixedly sleeved on the outer side surface of each constant temperature freezing block (20), and a second heat insulation layer (70) is fixedly sleeved on the annular side of the heat conducting block (30).

3. The program cooling device according to claim 1 or 2, wherein the distribution mode and the number of the constant temperature freezing blocks (20) can be set according to the requirement, and the temperature of each constant temperature freezing block (20) is decreased in turn in a clockwise or counterclockwise direction or a linear direction.

4. Procedure cooling device according to claim 1 or 2, characterised in that the circulation mechanism (50) comprises at least a rotating disc, on which each block (30) is arranged circularly and is fixedly mounted.

5. The program cooling device according to claim 1, wherein the ultra-low temperature storage mechanism (300) comprises:

a storage rack (310), the storage rack (310) forming a receiving space for storing the freezing storage tube (40);

a lifting mechanism (320), the storage rack (310) being connected to the lifting mechanism (320);

a freezing chamber (330), wherein the lifting mechanism (320) is arranged in the freezing chamber (330), and the lifting mechanism (320) can drive the storage rack (310) to enter or move out of the freezing chamber (330);

and the refrigerating piece (340), the refrigerating piece (340) is fixedly arranged on the outer surface of the freezing bin (330).

6. The process cooling device according to claim 1, further comprising a transfer mechanism (400) and an environmental control mechanism (500), the transfer mechanism (400) being used for the transfer of the cryopreserved sample; the environmental control mechanism (500) includes: the temperature and humidity controller comprises a regulation and control box, a primary filter, a centrifugal fan, a high-efficiency filter, a sensor assembly, a temperature and humidity controller and a sterilization actuator, and is used for keeping the environment state required by the program cooling equipment during operation.