CN215924960U - Stem cell culture perfusion device - Google Patents
Stem cell culture perfusion device Download PDFInfo
- Publication number
- CN215924960U CN215924960U CN202021415848.8U CN202021415848U CN215924960U CN 215924960 U CN215924960 U CN 215924960U CN 202021415848 U CN202021415848 U CN 202021415848U CN 215924960 U CN215924960 U CN 215924960U
- Authority
- CN
- China
- Prior art keywords
- resistor
- liquid level
- operational amplifier
- capacitor
- perfusion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a stem cell culture perfusion device, which comprises a perfusion bottle, a cell culture device, a cell perfusion groove and a waste liquid bottle, wherein a liquid level monitoring assembly is arranged at the top of the cell perfusion groove, the liquid level monitoring assembly comprises an infrared liquid level sensor for detecting the liquid level in the cell perfusion groove, the detection signal of the infrared liquid level sensor is sequentially sent into a band-pass preamplifier circuit and a filtering and isolating circuit for processing, the band-pass preamplifier circuit filters a foreign frequency signal outside the frequency of the liquid level detection signal by using the principle of a band-pass filter, so that the interference of an external high-frequency signal is effectively avoided, and the foreign frequency signal is further sent into the perfusion bottle after RC filtering to enhance the signal intensity; the filter isolation circuit utilizes the RC filter circuit principle to carry out low-pass noise reduction treatment, further promotes the precision of liquid level detection, then utilizes the voltage follower principle to keep apart the output to the AD converter with the detected signal, avoids preceding stage circuit to cause the interference to analog-to-digital conversion.
Description
Technical Field
The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture perfusion device.
Background
In the perfusion process, continuous media renewal is achieved by removing depleted culture and replenishing fresh media, while cells are trapped within the bioreactor by the cell separation device. When the perfusion process is actually operated, nutrients suitable for stem cell culture and propagation are filled in a perfusion bottle, supplementary nutrients are filled in a cell culture device, and the nutrients in the perfusion bottle flow into the cell culture device and a cell perfusion groove under the action of a peristaltic pump. However, in the process of nutrient supplement, even if the same peristaltic pump is used, the completely consistent and accurate flow rate cannot be provided, so that the liquid level in the cell perfusion groove is difficult to accurately control, and the stem cell culture process is not facilitated.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention aims to overcome the drawbacks of the prior art and provide a stem cell culture perfusion device.
The technical scheme for solving the problem is as follows: stem cell culture perfusion device, including perfusion bottle, cell culture ware, cell perfusion groove and waste liquid bottle, cell perfusion groove top sets up the liquid level monitoring subassembly, the liquid level monitoring subassembly is including the infrared level sensor who is used for detecting liquid level in the cell perfusion groove, infrared level sensor's detected signal is handled in sending into band-pass preamplifier circuit and filtering isolator circuit in proper order, filtering isolator circuit's output signal is in sending into the controller after the AD converter conversion, the controller is used for control cell perfusion groove both ends peristaltic pump operating condition.
Preferably, the band-pass preamplifier circuit comprises an operational amplifier AR1, an inverting input terminal of the operational amplifier AR1 is connected with one end of a resistor R2 and a capacitor C2, the other end of the resistor R2 is connected with one end of a resistor R1, a resistor R3 and a capacitor C1, the other end of the resistor R1 is connected with a signal output terminal of the infrared liquid level sensor, the other end of the capacitor C1 is grounded, the resistor R3, the other end of the capacitor C2 is connected with the output end of the operational amplifier AR1, the non-inverting input end of the operational amplifier AR1 is connected with one end of the resistors R4, R6 and C3 through the resistor R5, the other end of the resistor R4 is connected with a +5V power supply, the other ends of the resistors R6 and the capacitor C3 are grounded, the output end of the operational amplifier AR1 is connected with one end of the capacitor C4 and the inverting input end of the operational amplifier AR2 through the resistor R7, the other end of the capacitor C4 is grounded, the non-inverting input end of the operational amplifier AR2 is grounded through the resistor R8, and the output end of the operational amplifier AR2 is grounded through the resistor R9.
Preferably, the filter isolation circuit comprises a resistor R10, one end of the resistor R10 is connected to one end of a capacitor C5 and the output end of an operational amplifier AR2, the other end of the resistor R10 is connected to one end of a capacitor C6 and the non-inverting input end of an operational amplifier AR3, the other ends of the capacitors C5 and C6 are grounded, and the inverting input end and the output end of the operational amplifier AR3 are connected to the a/D converter.
Preferably, the controller is a single chip microcomputer.
Through the technical scheme, the utility model has the beneficial effects that:
1. the band-pass preamplifier circuit filters out the foreign frequency signals outside the frequency of the liquid level detection signal by using the principle of a band-pass filter, thereby effectively avoiding the interference of external high-frequency signals, and then the foreign frequency signals are sent to a further operational amplifier to enhance the signal intensity after being filtered by an RC (resistance-capacitance) filter;
2. the filter isolation circuit applies the RC filter circuit principle to carry out low-pass noise reduction on the output signal of the band-pass preamplifier circuit, the liquid level detection accuracy is further improved, then the voltage follower principle is applied to isolate and output the detection signal to the A/D converter, and interference of a preceding-stage circuit on analog-to-digital conversion is avoided.
Drawings
FIG. 1 is a schematic circuit diagram of the fluid level monitoring assembly of the present invention.
Detailed Description
The foregoing and other technical and scientific aspects, features and utilities of the present invention will be apparent from the following detailed description taken in conjunction with the accompanying drawings of fig. 1. The structural contents mentioned in the following embodiments are all referred to the attached drawings of the specification.
Exemplary embodiments of the present invention will be described below with reference to the accompanying drawings.
Stem cell culture perfusion device, including the perfusion bottle, the cell culture ware, cell perfusion groove and waste liquid bottle, cell perfusion groove top sets up the liquid level monitoring subassembly, the liquid level monitoring subassembly is including the infrared level sensor who is used for detecting liquid level in the cell perfusion groove, infrared level sensor's detected signal sends into band-pass preamplifier circuit and filtering isolation circuit in proper order and handles, filtering isolation circuit's output signal sends into the controller after the AD converter conversion, the singlechip is chooseed for use to the controller, through the operating condition of singlechip control cell perfusion groove both ends peristaltic pump.
In order to improve the accuracy of the detection result of the infrared liquid level sensor, firstly, a liquid level detection signal of the infrared liquid level sensor is sent to a band-pass preamplifier circuit for processing. As shown in fig. 1, the band-pass preamplifier circuit includes an operational amplifier AR1, an inverting input terminal of the operational amplifier AR1 is connected to one end of a resistor R2 and a capacitor C2, the other end of the resistor R2 is connected to one end of a resistor R1, a resistor R3 and a capacitor C1, the other end of the resistor R1 is connected to a signal output terminal of the infrared liquid level sensor, the other end of the capacitor C1 is grounded, the resistor R3, the other end of the capacitor C2 is connected with the output end of the operational amplifier AR1, the non-inverting input end of the operational amplifier AR1 is connected with one end of the resistors R4, R6 and C3 through the resistor R5, the other end of the resistor R4 is connected with a +5V power supply, the other ends of the resistors R6 and the capacitor C3 are grounded, the output end of the operational amplifier AR1 is connected with one end of the capacitor C4 and the inverting input end of the operational amplifier AR2 through the resistor R7, the other end of the capacitor C4 is grounded, the non-inverting input end of the operational amplifier AR2 is grounded through the resistor R8, and the output end of the operational amplifier AR2 is grounded through the resistor R9.
In the working process of the band-pass preamplifier circuit, the resistors R2 and R3 and the capacitors C1 and C2 form a second-order RC band-pass filter network at the periphery of the operational amplifier AR1, and a band-pass filter principle is used for filtering out the impurity frequency signals outside the liquid level detection signal frequency, so that the interference of external high-frequency signals, such as high-frequency noise generated by an external environment light source, is effectively avoided. The output signal of the operational amplifier AR1 is RC filtered and then sent to the operational amplifier AR2 for further enhancement and then sent to the filter isolation circuit.
The filter isolation circuit comprises a resistor R10, one end of the resistor R10 is connected with one end of a capacitor C5 and the output end of an operational amplifier AR2, the other end of the resistor R10 is connected with one end of a capacitor C6 and the non-inverting input end of the operational amplifier AR3, the other ends of the capacitors C5 and C6 are grounded, and the inverting input end and the output end of the operational amplifier AR3 are connected with an A/D converter. The resistor R10 and the capacitors C5 and C6 form a pi-type RC filter circuit to perform low-pass noise reduction on the output signal of the operational amplifier AR2, and the liquid level detection accuracy is further improved. And finally, the operational amplifier AR3 isolates and outputs the detection signal to the A/D converter by using the principle of a voltage follower, so that the interference of a preceding-stage circuit on the analog-to-digital conversion is avoided.
When the cell perfusion tank is used specifically, the infrared liquid level sensor detects the liquid level in the cell perfusion tank in real time, and the band-pass preamplifier circuit filters out the foreign frequency signals outside the frequency of the liquid level detection signals by using the principle of a band-pass filter, so that the interference of external high-frequency signals is effectively avoided, and the signals are filtered by RC and then are sent to a further operational amplifier to enhance the signal intensity. The filter isolation circuit applies the RC filter circuit principle to carry out low-pass noise reduction on the output signal of the band-pass preamplifier circuit, the liquid level detection accuracy is further improved, then the voltage follower principle is applied to isolate and output the detection signal to the A/D converter, and interference of a preceding-stage circuit on analog-to-digital conversion is avoided. The single chip microcomputer adjusts the working state of the peristaltic pump according to the real-time liquid level data value, so that the liquid level in the cell perfusion groove is always kept within a set range.
While the utility model has been described in further detail with reference to specific embodiments thereof, it is not intended that the utility model be limited to the specific embodiments thereof; for those skilled in the art to which the present invention pertains and related technologies, the extension, operation method and data replacement should fall within the protection scope of the present invention based on the technical solution of the present invention.
Claims (3)
1. Stem cell culture perfusion device, including perfusion bottle, cell culture ware, cell perfusion groove and waste liquid bottle, its characterized in that: a liquid level monitoring assembly is arranged at the top of the cell perfusion tank, the liquid level monitoring assembly comprises an infrared liquid level sensor for detecting the liquid level in the cell perfusion tank, detection signals of the infrared liquid level sensor are sequentially sent to a band-pass preamplifier circuit and a filtering and isolating circuit for processing, output signals of the filtering and isolating circuit are converted by an A/D converter and then sent to a controller, and the controller is used for controlling the working state of peristaltic pumps at two ends of the cell perfusion tank; the band-pass preamplifier circuit comprises an operational amplifier AR1, an inverting input end of the operational amplifier AR1 is connected with one end of a resistor R2 and one end of a capacitor C2, the other end of a resistor R2 is connected with one end of a resistor R1, one end of a resistor R3 and one end of a capacitor C1, the other end of a resistor R1 is connected with a signal output end of the infrared liquid level sensor, the other end of a capacitor C1 is grounded, the other ends of the resistor R1 and the capacitor C1 are connected with an output end of the operational amplifier AR1, a non-inverting input end of the operational amplifier AR1 is connected with one end of the resistor R1, one end of the capacitor R1 and one end of the capacitor C1 through the resistor R1, the other end of the resistor R1 is connected with a +5V power supply, the other ends of the resistor R1 and the capacitor C1 are grounded, an output end of the operational amplifier AR1 is connected with one end of the capacitor C1 and the inverting input end of the operational amplifier AR1 through the resistor R1.
2. The stem cell culture perfusion apparatus of claim 1, wherein: the filter isolation circuit comprises a resistor R10, one end of the resistor R10 is connected with one end of a capacitor C5 and the output end of an operational amplifier AR2, the other end of the resistor R10 is connected with one end of a capacitor C6 and the non-inverting input end of the operational amplifier AR3, the other ends of the capacitors C5 and C6 are grounded, and the inverting input end and the output end of the operational amplifier AR3 are connected with the A/D converter.
3. The stem cell culture perfusion apparatus of claim 1, wherein: the controller is a single chip microcomputer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021415848.8U CN215924960U (en) | 2020-07-17 | 2020-07-17 | Stem cell culture perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021415848.8U CN215924960U (en) | 2020-07-17 | 2020-07-17 | Stem cell culture perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215924960U true CN215924960U (en) | 2022-03-01 |
Family
ID=80408883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021415848.8U Active CN215924960U (en) | 2020-07-17 | 2020-07-17 | Stem cell culture perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215924960U (en) |
-
2020
- 2020-07-17 CN CN202021415848.8U patent/CN215924960U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN212058990U (en) | Surface water quality treatment monitoring system | |
| CN105536105A (en) | Infusion set monitoring system and monitoring method | |
| CN215924960U (en) | Stem cell culture perfusion device | |
| CN110117136A (en) | Garbage percolation liquid treating system and method | |
| CN100364637C (en) | Intelligent vacuum filtering instrument and its control method | |
| CN215480211U (en) | Sewage treatment equipment capable of monitoring PAC (programmable automation controller) adding amount in real time | |
| CN102221497A (en) | Water sample pretreatment device | |
| CN207493510U (en) | Water management system | |
| CN105597166A (en) | Gradient-type negative-pressure suction method and treatment device based on gradient negative-pressure suction method | |
| CN217278703U (en) | Cable thermal ageing case temperature control system | |
| CN202632061U (en) | Automatic reagent adding device for neutralizing treatment of acid-base waste water of heat-engine plant | |
| CN209481652U (en) | A kind of on-line monitoring system of anaerobic reactor Inlet and outlet water volatile fatty acid | |
| CN114136835A (en) | Method for detecting content of suspended matters in sewage | |
| CN203909029U (en) | Servo device of on-line automatic water quality safety monitoring system | |
| CN202793480U (en) | Zero correction device for electromagnetic flowmeter | |
| CN206607093U (en) | A kind of printing wastewater purifies reutilization system | |
| CN108872329A (en) | Oil concentration measuring instrument in a kind of water | |
| CN213668726U (en) | Cleaning system with filter membrane component | |
| CN206109366U (en) | Novel fruit wine clarification filtration system | |
| CN201990658U (en) | Fruit vinegar settling and compound device system | |
| CN205450003U (en) | Full -automatic oil -smoke testing equipment | |
| CN211877180U (en) | Animal remedy production pure water system water level monitoring device | |
| CN217092752U (en) | Take on-line monitoring's exhaust-gas treatment equipment | |
| CN218243743U (en) | Medical intermediate waste water treatment monitoring system | |
| CN102774956A (en) | Intelligent water environment microbe enriching equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |