CN102774956A - Intelligent water environment microbe enriching equipment - Google Patents
Intelligent water environment microbe enriching equipment Download PDFInfo
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- CN102774956A CN102774956A CN2012102639345A CN201210263934A CN102774956A CN 102774956 A CN102774956 A CN 102774956A CN 2012102639345 A CN2012102639345 A CN 2012102639345A CN 201210263934 A CN201210263934 A CN 201210263934A CN 102774956 A CN102774956 A CN 102774956A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention relates to intelligent water environment microbe enriching equipment, which comprises an enriching device and a control device, wherein the enriching device is characterized in that a first water storage tank is sequentially connected with a first electromagnetic valve, a first peristaltic pump and a first filtering post, a second water storage tank is sequentially connected with a second electromagnetic valve, a second peristaltic pump and a second filtering post, an eluent tank is connected with a third electromagnetic valve, and a third electromagnetic valve is connected with a first electromagnetic valve and a second electromagnetic valve; and the control device is characterized in that an analog module is connected with the output of a PLC (programmable logic controller), the input of the PLC is connected with a first flow rate sensor and a second flow rate sensor, the output of the PLC is connected with the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and a relay, the output of the analog module is connected with a control module arranged on the first peristaltic pump and the second peristaltic pump, a switch contract of the relay is connected with the control module, the first flow rate sensor is arranged on a connecting pipeline of the first electromagnetic valve and the first peristaltic pump, the second flow rate sensor is arranged on a connecting pipeline of the second electromagnetic valve and the second peristaltic pump, and the touch screen is connected with the PLC. The intelligent water environment microbe enriching equipment has the advantages that the operation is simple, convenient and safe, and the water body enriching process is intelligently controlled.
Description
Technical field
The present invention relates to a kind of water surrounding microbial enrichment equipment.Particularly relate to a kind of operator and do not have the intellectual water surrounding pathogenic micro-organism enrichment plant that directly contacts with the various experiment water samples of biological pollution.
Background technology
The various pathogenic micro-organisms that exist in the water surrounding (comprising Causative virus and malignant bacteria) have caused great harm to human health; Not only can cause collective's eruption and prevalence of cholera, viral hepatitis, poliomyelitis, typhoid fever and water transmissible diseases such as typhus fever, infectious diarrhea, but also often cause extremely abominable harm and social detrimentally affect.2006, China reported Notifiable disease number of the infected 460.9 ten thousand, wherein, leaned on water-borne number of the infected to reach 127.8 ten thousand, accounted for 27.7%; In addition, according to The World Health Organization (WHO) report, whole world every year is immediate cause because of dead 2,200,000 people of infectious diarrhea (account for death 4%) and drink by the water of bacterium, virus infection.U.S. WBDOSS (Waterborne Disease and Outbreak Surveillance System) data also show; During the 1971-2006; 780 drinking water pollutions in U.S.'s outburst cause in the case of water illness outbreak; Just have 680 to pollute relevantly with public water system, wherein, the biotic pollution incident accounts for 43.9%.Therefore, must carry out efficiently and fast monitoring, block the approach of its propagation the pathogenic micro-organism in the various water bodys such as tap water, surface water, reuse water.
Generally speaking, the pathogenic micro-organism content in the water surrounding is considerably less, far below the detection sensitivity of existing monitoring method.Therefore, if think to detect effectively the pathogenic micro-organism in the water, at first must be to processings of concentrating of a large amount of water samples, from different water bodys with a spot of pathogenic micro-organism enrichment, reclaim, for laboratory and/or the on-the-spot usefulness that detects.At present; The enriching method of disease caused by infectious water pathogenic microorganism mainly is based upon laboratory level; Comprise filtration method, ultrafiltration process, flocculence, absorb-elute method etc., generally speaking, can choose diverse ways according to different experiment purposes, experiment water body and test conditions.
The absorb-elute method is the at present big water by volume appearance pathogenic micro-organism the most frequently used method that concentrates, and it is through being adsorbed on pathogenic micro-organism on the surface of filter membrane material or solid particulate, and then wash-out reclaims pathogenic micro-organism.Wherein, Adopting solid particulate (positive charge particulate filter, glass powder, silica gel, zeyssatite etc.) to carry out absorb-elute receives the water sample opacity to influence characteristics such as not obvious, that the pathogenic micro-organism recovery is higher, the processing water yield is big, easy and simple to handle; Be widely used, be particularly suitable for the enrichment of various water body pathogenic micro-organisms such as big volume tap water, surface water, reuse water.
Yet at present, this method basically all is manual operations, and operator inevitably need directly contact with various water bodys at experimentation.In recent years; Along with the pay attention to day by day of China to Biosafety; Also more and more stricter to the requirement of operator safety property, as how effectively to protect operator, to reduce operator and biological pollution water sample (water body that especially contains the high density pathogenic bacteria) as far as possible direct one of problem of also becoming the urgent solution of pathogenic micro-organism beneficiation technologies in the developing water environment that contacts.Therefore, research and develop intellectual water surrounding pathogenic micro-organism enrichment plant and have profound significance and vast market demand with Biosafety.Yet, also do not see the appearance of relevant device at present both at home and abroad.
Summary of the invention
Technical problem to be solved by this invention is that a kind of intellectual water surrounding pathogenic micro-organism enrichment plant of when enrichment pathogenic micro-organism process, avoiding the operator and the various water sources of biological pollution directly to contact is provided.
The technical scheme that the present invention adopted is: a kind of intellectual water surrounding pathogenic micro-organism enrichment plant comprises enriching apparatus and gear.
Described enriching apparatus comprises: first water storing tank, second water storing tank and wash-out flow container, and described first water storing tank is connected with first SV, first peristaltic pump, the first filter post through pipeline successively; Second water storing tank is connected with second SV, second peristaltic pump, the second filter post through pipeline successively; The wash-out flow container is connected with the 3rd SV through pipeline, and described the 3rd SV connects first SV and second SV through pipeline respectively;
Described gear comprises: PLC, touch-screen, analog module, rly., first-class speed sensors and second flow rate sensor; The signal input part of described analog module is connected with the signal output part of PLC; The signal input part of described PLC connects the first-class speed sensors and second flow rate sensor respectively; The signal output part of described PLC connects first SV, second SV and the 3rd SV respectively; And connection rly.; The signal output part of said analog module connects the signal input part that is separately positioned on the control module on first peristaltic pump and second peristaltic pump, and the switch contact of said rly. connects the power input that is separately positioned on the control module on first peristaltic pump and second peristaltic pump, and described first-class speed sensors is arranged on first SV and the pipeline that first peristaltic pump is connected; Second flow rate sensor is arranged on second SV and the pipeline that second peristaltic pump is connected, and described touch-screen links to each other with PLC.
The set inside of the first filter post and the second filter post has filtrate or film.
Said filtrate is positive-charge charged filtrate, spun glass, pitch coal particle, resin particle, glass powder particles, silica gel particle or diatomite particle.
Said film is cellulose mixture film or polyester cellulose film.
Suitable water body comprises tap water, pure water, well water, reuse water, secondary effluent, surface water.
The volume of handling water sample is 10-1000L.
Suitable water body also comprises municipal effluent and sewage, and the volume of handling water sample is 5-20L.
The aqueous sample stream speed control is in 50L/h.
Intellectual water surrounding pathogenic micro-organism enrichment plant of the present invention has following characteristics:
1, operator directly contact with the nothing of the various experiment water samples of biological pollution;
2, easy and simple to handle, safety, intelligent controlling microorganisms in water enrichment process;
3, can handle various water bodies such as tap water, pure water, well water, reuse water, secondary effluent, surface water, municipal effluent, sewage;
4, the processing volume of water sample is big;
5, can handle two kinds of water samples simultaneously.
Description of drawings
Fig. 1 is the whole block diagram that constitutes of the present invention;
Fig. 2 is the formation block diagram of gear of the present invention;
Fig. 3 is the schematic circuit diagram of PLC load module of the present invention;
Fig. 4 is the schematic circuit diagram of PLC output module of the present invention;
Fig. 5 is the schematic circuit diagram of analog module of the present invention;
Fig. 6 is the schematic circuit diagram of peristaltic pump control module of the present invention.
2: the second SVs of 1: the first SV
4: the first peristaltic pumps of 3: the three SVs
5: the second peristaltic pumps filter in 6: the first post
8: the first water storing tanks of 7: the second filter posts
9: 10: the second water storing tanks of wash-out flow container
11: 12: the second flow rate sensors of first-class speed sensors
13: enriching apparatus 14:PLC
15: touch-screen 16: analog module
17: rly. 18: gear
Embodiment
Below in conjunction with embodiment and accompanying drawing intellectual water surrounding pathogenic micro-organism enrichment plant of the present invention is made detailed description.
Intellectual water surrounding pathogenic micro-organism enrichment plant of the present invention, through PLC, analog module, touch-screen intelligent control pathogenic micro-organism enrichment process, operator directly contact with the nothing of the various experiment water samples of biological pollution.Specifically include enriching apparatus 13 and gear 18, as shown in Figure 1, wherein,
Described enriching apparatus 13 comprises: first water storing tank 8, second water storing tank 10 and wash-out flow container 9, and described first water storing tank 8 is connected with first SV 1, first peristaltic pump 4, the first filter post 6 through pipeline successively; Second water storing tank 10 is connected with second SV 2, second peristaltic pump 5, the second filter post 7 through pipeline successively; Wash-out flow container 9 is connected with the 3rd SV 3 through pipeline, and described the 3rd SV 3 connects first SV 1 and second SV 2 through pipeline respectively.
The set inside of the first filter post 6 and the second filter post 7 has filtrate or film.
Said filtrate is positive-charge charged filtrate, spun glass, pitch coal particle, resin particle, glass powder particles, silica gel particle or diatomite particle.Said film is cellulose mixture film or polyester cellulose film.
Described gear 18 comprises: PLC14, touch-screen 15, analog module 16, rly. 17, first-class speed sensors 11 and second flow rate sensor 12; The signal input part of described analog module 16 is connected with the signal output part of PLC14; The signal input part of described PLC14 connects the first-class speed sensors 11 and second flow rate sensor 12 respectively; The signal output part of described PLC14 connects first SV 1, second SV 2 and the 3rd SV 3 respectively; And connection rly. 17; The signal output part of said analog module 16 connects the signal input part that is separately positioned on the control module on first peristaltic pump 4 and second peristaltic pump 5; The switch contact of said rly. 17 connects the power input that is separately positioned on the control module on first peristaltic pump 4 and second peristaltic pump 5; Described first-class speed sensors 11 is arranged on first SV 1 and the pipeline that first peristaltic pump 4 is connected, and second flow rate sensor 12 is arranged on second SV 3 and the pipeline that second peristaltic pump 5 is connected, and described touch-screen 15 links to each other with PLC14.Described PLC14, touch-screen 15, analog module 16, rly. 17 are installed in the electric appliance control box.
Like Fig. 2～shown in Figure 6, in the present embodiment, the model of described PLC14 is FX1N24MR; The model of touch-screen 15 is MT8070iH; The model of analog module 16 is FX2N-2DA; The model of peristaltic pump control module is MT600-2J.
After PLC14 is connected to the first-class speed sensors 11 of on-line monitoring and second flow rate sensor 12 and online acquisition pulse on the enriching apparatus 13 through data line; Through feeding back on the touch-screen 15 through data line again after its internal calculation processing, demonstrate the online flow velocity of liquid in real time; Analog module 16 receives the instruction of PLC14; The voltage of output 1～10V also is connected on the control module of first peristaltic pump 4 and second peristaltic pump 5 in the enriching apparatus 13 through data line, controls the rotating speed of first peristaltic pump 4 and second peristaltic pump 5 through the control module internal processes; PLC14 is also connected on first SV 1, second SV 2 and the 3rd SV 3 of enriching apparatus 13 through data line; Be connected the line of instruction data on the touch-screen 15 through PLC14 simultaneously; Accept the key command on the touch-screen 15, thus the switch of control SV; The switch contact of rly. 17 is connected on the power switch of enriching apparatus 13 peristaltic pumps.
Intellectual water surrounding pathogenic micro-organism enrichment plant of the present invention, the aqueous sample stream speed control is in 50L/h.Suitable water body comprises the water body that tap water, pure water, well water, reuse water, secondary effluent, surface water etc. are comparatively clean, and the volume of handling water sample is 10-1000L.Suitable water body also comprises sewage and municipal effluent, and for sewage and municipal effluent, the volume of handling water sample is 5-20L.
Provide the example of actual use below:
The enrichment of poliovirus in the embodiment 15L municipal effluent
Get sewage treatment plant inflow 10L, behind 121 ℃ of autoclaving 20min, add poliovirus, making its final titre is 10
5PFU/mL.Each 5L distinguishes in first water storing tank 8 and second water storing tank, 10 water sample buckets of adding apparatus with this artificial contamination's water sample, and elutriant is put into wash-out flow container 9 buckets.Simultaneously, populated filtrate in the first filter post 6 and the second filter post 7.Carry out the enrichment of virus in the sewage according to following working method:
1, after the device power, presses touch-screen 15 and get into os, will get into main menu;
2, at the main menu of touch-screen 15, setup parameter, realize each function: at first rotating speed setting, get into No. 1 station and No. 2 stations respectively, press " rotating speed " button, rotating speed is set is 50L/h and remember; Then, " water sample " in pressing the button and " startup peristaltic pump ", two workplatformes of equipment all begin the drainage and the virus that concentrates simultaneously, and the water sample after the filtration flows away from pipeline;
3, treat that whole filtration of water sample finishes, and presses " stopping peristaltic pump " button;
4, reset rotating speed: press " rotating speed " button, it is 20mL/min and memory that rotating speed is set; Press " elutriant ", " collection ", " startup peristaltic pump " in standing and stand for No. 2 for No. 1 successively;
5, shutdown: pressing the touch-screen main menu, buttons such as " peristaltic pump stops ", " water sample elutriant ", " power switch " cut out, can shut down.
The recovery of poliovirus is measured and calculated to virus titer in the elutriant, and the result shows that this equipment is more than 95% the recovery of poliovirus in 2 parts of 5L sewage.
The enrichment of poliovirus in the embodiment 210L tap water
Get tap water 20L, behind 121 ℃ of autoclaving 20min, add poliovirus, making its final titre is 105PFU/mL.This artificial contamination's tap water 10L is distinguished in first water storing tank 8 and second water storing tank, 10 water sample buckets of adding apparatus, and elutriant is put into wash-out flow container 9 buckets.Simultaneously, populated filtrate in the first filter post 6 and the second filter post 7.Working method is carried out according to embodiment 1.This equipment is 98% to the recovery of poliovirus in 2 parts of 10L tap water.The enrichment of poliovirus in the embodiment 3100L tap water
Get tap water 100L, behind 121 ℃ of autoclaving 20min, add poliovirus, making its final titre is 10
5PFU/mL.This artificial contamination's tap water is added in second water storing tank, the 10 water sample buckets, and elutriant is put into wash-out flow container 9 buckets.Simultaneously, populated filtrate in the second filter post 7.Working method is carried out according to embodiment 1, but only need start No. 2 station work.This equipment reaches more than 90% the recovery of poliovirus in the 100L tap water.
The enrichment of poliovirus in the embodiment 4 100L reuse waters
Fetch water 100L, behind 121 ℃ of autoclaving 20min, add poliovirus, making its final titre is 10
5PFU/mL.This artificial contamination's reuse water is added in second water storing tank, the 10 water sample buckets, and elutriant is put into wash-out flow container 9 buckets.Simultaneously, populated filtrate in the second filter post 7.Working method is carried out according to embodiment 1, but only need start No. 2 station work.This equipment reaches more than 90% the recovery of poliovirus in the 100L reuse water.
The enrichment of poliovirus in the embodiment 5 100L secondary effluents
Get secondary effluent 100L, behind 121 ℃ of autoclaving 20min, add poliovirus, making its final titre is 10
5PFU/mL.This artificial contamination's reuse water is added in second water storing tank, the 10 water sample buckets, and elutriant is put into wash-out flow container 9 buckets.Simultaneously, populated filtrate in the second filter post 7.Working method is carried out according to embodiment 1, but only need start No. 2 station work.This equipment reaches more than 90% the recovery of poliovirus in the 100L secondary effluent.
The enrichment of poliovirus in the embodiment 6 100L surface water
Get surface water 100L, behind 121 ℃ of autoclaving 20min, add poliovirus, making its final titre is 105PFU/mL.In first water storing tank, the 8 water sample buckets with this artificial contamination's surface water adding apparatus, and elutriant put into wash-out flow container 9 buckets.Simultaneously, populated filtrate in the first filter post 6.Working method is carried out according to embodiment 1, but only need start No. 1 station work.This equipment reaches more than 90% the recovery of poliovirus in the 100L surface water.
Claims (8)
1. an intellectual water surrounding pathogenic micro-organism enrichment plant comprises enriching apparatus (13) and gear (18), it is characterized in that:
Described enriching apparatus (13) comprising: first water storing tank (8), second water storing tank (10) and wash-out flow container (9), and described first water storing tank (8) is connected with first SV (1), first peristaltic pump (4), the first filter post (6) through pipeline successively; Second water storing tank (10) is connected with second SV (2), second peristaltic pump (5), the second filter post (7) through pipeline successively; Wash-out flow container (9) is connected with the 3rd SV (3) through pipeline, and described the 3rd SV (3) connects first SV (1) and second SV (2) through pipeline respectively;
Described gear (18) comprising: PLC (14), touch-screen (15), analog module (16), rly. (17), first-class speed sensors (11) and second flow rate sensor (12); The signal input part of described analog module (16) is connected with the signal output part of PLC (14); The signal input part of described PLC (14) connects first-class speed sensors (11) and second flow rate sensor (12) respectively; The signal output part of described PLC (14) connects first SV (1), second SV (2) and the 3rd SV (3) respectively; And connection rly. (17); The signal output part of said analog module (16) connects the signal input part that is separately positioned on the control module on first peristaltic pump (4) and second peristaltic pump (5); The switch contact of said rly. (17) connects the power input that is separately positioned on the control module on first peristaltic pump (4) and second peristaltic pump (5); Described first-class speed sensors (11) is arranged on first SV (1) and the pipeline that first peristaltic pump (4) is connected; Second flow rate sensor (12) is arranged on second SV (3) and the pipeline that second peristaltic pump (5) is connected, and described touch-screen (15) links to each other with PLC (14).
2. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 1 is characterized in that, the set inside of the first filter post (6) and the second filter post (7) has filtrate or film.
3. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 2 is characterized in that said filtrate is positive-charge charged filtrate, spun glass, pitch coal particle, resin particle, glass powder particles, silica gel particle or diatomite particle.
4. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 2 is characterized in that said film is cellulose mixture film or polyester cellulose film.
5. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 1 is characterized in that suitable water body comprises tap water, pure water, well water, reuse water, secondary effluent, surface water.
6. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 5 is characterized in that, the volume of handling water sample is 10-1000L.
7. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 1 is characterized in that suitable water body also comprises municipal effluent and sewage, and the volume of handling water sample is 5-20L.
8. intellectual water surrounding pathogenic micro-organism enrichment plant according to claim 1 is characterized in that the aqueous sample stream speed control is in 50L/h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102639345A CN102774956A (en) | 2012-07-19 | 2012-07-19 | Intelligent water environment microbe enriching equipment |
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| CN2012102639345A CN102774956A (en) | 2012-07-19 | 2012-07-19 | Intelligent water environment microbe enriching equipment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913544A (en) * | 2018-08-02 | 2018-11-30 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment |
| CN113720667A (en) * | 2021-09-02 | 2021-11-30 | 艾萍 | Water sample treatment system and method for environmental monitoring |
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| CN101928668A (en) * | 2009-06-23 | 2010-12-29 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Technology for highly-efficiently enriching pathogenic bacteria in water environments |
| CN102235948A (en) * | 2010-04-27 | 2011-11-09 | 天津市欧诺仪器仪表有限公司 | Quick virus enrichment method and enrichment device for environmental water sample |
| CN202785784U (en) * | 2012-07-19 | 2013-03-13 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Intelligent water environment pathogenic microorganism enrichment device |
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2012
- 2012-07-19 CN CN2012102639345A patent/CN102774956A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928668A (en) * | 2009-06-23 | 2010-12-29 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Technology for highly-efficiently enriching pathogenic bacteria in water environments |
| CN102235948A (en) * | 2010-04-27 | 2011-11-09 | 天津市欧诺仪器仪表有限公司 | Quick virus enrichment method and enrichment device for environmental water sample |
| CN202785784U (en) * | 2012-07-19 | 2013-03-13 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Intelligent water environment pathogenic microorganism enrichment device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108913544A (en) * | 2018-08-02 | 2018-11-30 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Pathogenic microorganism efficiently concentrating device and its enrichment method in a kind of water environment |
| CN113720667A (en) * | 2021-09-02 | 2021-11-30 | 艾萍 | Water sample treatment system and method for environmental monitoring |
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Application publication date: 20121114 |