CN215328071U - Mesenchymal stem cell filtering separator - Google Patents
Mesenchymal stem cell filtering separator Download PDFInfo
- Publication number
- CN215328071U CN215328071U CN202121302392.9U CN202121302392U CN215328071U CN 215328071 U CN215328071 U CN 215328071U CN 202121302392 U CN202121302392 U CN 202121302392U CN 215328071 U CN215328071 U CN 215328071U
- Authority
- CN
- China
- Prior art keywords
- filter screen
- filter
- stem cell
- mesenchymal stem
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Centrifugal Separators (AREA)
Abstract
The application discloses mesenchymal stem cell filters separator, including chassis, knockout drum and separator and filter equipment, separator includes support, thread groove, top cap, motor, pinion, movable rod, fixed case, gear wheel, first filter screen, transfer line, toper fill, drain pipe and second filter screen, the top fixed connection support on chassis, the top fixed connection knockout drum of support, the thread groove has been seted up at the top of knockout drum. This application is convenient once to filter the mixture through dog and first filter screen, and article after the filtration pass first filter screen and fall into on the second filter screen, and through starter motor, the motor drives transfer line and gear wheel rotation, and the gear wheel drives the pinion that the meshing is connected and rotates, and the pinion drives the transfer line and rotates, and the transfer line drives second filter screen and toper fill rotation to the centrifugal separation of mixture with higher speed.
Description
Technical Field
The application relates to the technical field of cell separation, in particular to a mesenchymal stem cell filtering separator.
Background
With the deep development of research and application of stem cells and tissue engineering technology, the separation and preparation of stem cells are increasingly regarded as one of key technologies in the field, the development of a separation device for leukocytes and tumor cells used in the medical field is fast so far, but the separation equipment for mesenchymal stem cells is few, the most applied separation device for mesenchymal stem cells is a cell screen, a stainless steel screen with 100-300 meshes is generally selected, and the purpose of separating the cells from digested tissue blocks is achieved by shaking, stirring and vibrating.
At present mesenchymal stem cell separation generally adopts the screen cloth to reach the purpose of cell and the tissue piece separation after the digestion through rocking, stirring and shaking, and its separation efficiency is low, and stem cell results volume is few, wastes time and energy to expose the operation for a long time, stem cell pollution chance greatly increased, inconvenient rapid separation and filtration. Therefore, a mesenchymal stem cell filter separator is provided to solve the above problems.
Disclosure of Invention
The mesenchymal stem cell filtering separator is used for solving the problems of low separation efficiency, less stem cell harvesting amount, time and labor waste, long-time exposure operation, greatly increased stem cell pollution opportunity, inconvenient rapid separation and filtration.
According to one aspect of the present application, there is provided a mesenchymal stem cell filtration separator comprising a base plate, a separation tank, and a separation device and a filtration device;
the separation device comprises a support, a thread groove, a top cover, a motor, a pinion, a movable rod, a fixed box, a gearwheel, a first filter screen, a transmission rod, a conical hopper, a drain pipe and a second filter screen, wherein the top end of the chassis is fixedly connected with the support, the top of the support is fixedly connected with a separation tank, and the top of the separation tank is provided with the thread groove;
the filter device comprises a feed inlet, a first filter screen, a discharge hopper, a communicating groove, a discharge pipe, a filter pipe, a third filter screen, a separating pipe, a valve, a bottom cover and a stop block, wherein the bottom end of the separating tank is fixedly connected with the discharge hopper, the bottom of the discharge hopper is communicated with the discharge pipe, and the bottom of the discharge pipe is communicated with the filter pipe.
Further, the top of knockout drum passes through thread groove threaded connection top cap, the feed inlet has been seted up at the top of top cap, the motor is installed on the top of top cap, the inside intercommunication drain pipe of one end of knockout drum, the intercommunication groove has been seted up to the bottom of knockout drum.
Further, the bottom fixed connection of top cap is fixed case, the inside of fixed case rotates and connects the movable rod, the movable rod cup joints the output at the motor.
Furthermore, a bull gear is sleeved at one end of the movable rod and meshed with a pinion, the pinion is sleeved at one end of the transmission rod, and one end of the transmission rod is rotatably connected to the inside of the fixed box.
Further, the inside fixed connection dog of knockout drum, first filter screen has been placed on the top of dog, the center of first filter screen is rotated and is connected with the transfer line, the bottom fixed connection second filter screen of transfer line, the top fixed connection toper fill of second filter screen.
Further, the inside fixed connection third filter screen of filter tube, the bottom intercommunication separator tube of filter tube, the internally mounted of separator tube has the valve, the bottom of separator tube is installed the bottom.
Through the above-mentioned embodiment of this application, adopted separator and filter equipment, solved the separation efficiency low, stem cell results volume is few, wastes time and energy to expose the operation for a long time, stem cell pollutes chance greatly increased, inconvenient quickly separating and filterable problem.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive exercise.
FIG. 1 is a schematic overall perspective view of an embodiment of the present application;
FIG. 2 is a schematic overall structure diagram of an embodiment of the present application;
fig. 3 is a schematic structural diagram of a separation tank, a drain pipe, a communication groove and a stopper according to an embodiment of the present application.
In the figure: 1. the device comprises a base plate, 2, a support, 3, a separation tank, 4, a thread groove, 5, a top cover, 6, a feeding hole, 7, a motor, 8, a pinion, 9, a movable rod, 10, a fixed box, 11, a big gear, 12, a first filter screen, 13, a transmission rod, 14, a conical hopper, 15, a drainage pipe, 16, a second filter screen, 17, a discharging hopper, 18, a communication groove, 19, a discharging pipe, 20, a filter pipe, 21, a third filter screen, 22, a separation pipe, 23, a valve, 24, a bottom cover, 25 and a stop block.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In this application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "mounted," "disposed," "provided," "connected," and "sleeved" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.
The filtering separator in this embodiment may be suitable for separation and filtration after extraction of mesenchymal stem cells, for example, the following filtering separator is provided in this embodiment, and the filtering separator in this embodiment may be used for separation and filtration after extraction of mesenchymal stem cells.
A mesenchymal stem cell extraction device comprises a strong sucker, a base, a support column and a spring column, wherein the base is connected on the strong sucker and used for supporting a tank body, the strong sucker is connected with the base through a screw, the support column is connected on the outer side of the base and used for supporting the tank body to prevent the tank body from inclining, the spring column is connected on the base and used for increasing the vibration intensity of the tank body, the tank body is installed on the spring column and used for extracting stem cells, a mounting frame is installed below the tank body and used for installing a vibration motor, the tank body is connected with the mounting frame through a bolt, the vibration motor is installed on the lower portion of the mounting frame and used for enabling the tank body to vibrate to extract the stem cells, the vibration motor is connected with the mounting frame through a bolt, a first electromagnetic valve is arranged on one side of the vibration motor and used for controlling the on-off of an extraction tube, one side of the first electromagnetic valve is connected with an extraction tube, the extraction tube is used for connecting or installing an extraction bottle to extract stem cells, the first electromagnetic valve is connected with the extraction tube through threads, the lower part of the outer side of the tank body is connected with a stabilizing ring used for reducing the shaking of the tank body, the tank body is connected with the stabilizing ring through viscose, one side of the tank body is connected with a fourth electromagnetic valve used for controlling the opening and closing of a liquid nitrogen outlet tube, the tank body is connected with the fourth electromagnetic valve through screws, one side of the fourth electromagnetic valve is connected with a liquid nitrogen outlet tube used for discharging liquid nitrogen in the tank body and circulating the liquid nitrogen in the tank body, the fourth electromagnetic valve is connected with the outlet tube through threads, a sealing cover is arranged on the upper surface of the tank body and used for sealing the tank body, a threaded column is connected below the sealing cover and used for sealing the tank body, the sealing cover is connected with the threaded column through screws, and the upper part of the outer side of the threaded column is connected with a sealing ring, the sealing ring is used for matching with a threaded column sealing tank body, a filter screen is installed on the inner side of the tank body and used for filtering stem cells, the tank body is connected with the filter screen through screws, a flow guide disc is arranged below the filter screen and used for guiding the stem cells to a first electromagnetic valve, a cover opening column is installed above the sealing cover and used for opening the sealing cover, the cover opening column is connected with the sealing cover through screws, a handle is arranged in front of the cover opening column and used for conveniently taking up the sealing cover and connected with the sealing cover through screws, a second electromagnetic valve is arranged on one side of the handle and used for controlling the opening and closing of a tissue inlet pipe, a tissue inlet pipe is connected above the second electromagnetic valve and used for separating the mesenchymal tissues to be separated from the stem cells, the second electromagnetic valve is connected with the tissue inlet pipe through threads, and a third electromagnetic valve is arranged on the other side of the handle, the third solenoid valve is used for controlling the switch that the liquid nitrogen entered the pipe, and the higher authority of third solenoid valve is connected with the liquid nitrogen and advances the pipe, and the liquid nitrogen advances the pipe and is used for letting in the liquid nitrogen, and the third solenoid valve advances the pipe with the liquid nitrogen and passes through threaded connection, and one side of liquid nitrogen exit tube is provided with the control box, and the control box is used for controlling means to move, and the control box includes the switch.
Of course, the present embodiment can also be used for separation and filtration after extraction of mesenchymal stem cells. The following describes the filtering separator according to the embodiment of the present application, which is not described in detail herein.
Referring to fig. 1 to 3, a mesenchymal stem cell filter separator includes a base plate 1, a separation tank 3, a separation device and a filtering device;
the separation device comprises a support 2, a thread groove 4, a top cover 5, a motor 7, a pinion 8, a movable rod 9, a fixed box 10, a gearwheel 11, a first filter screen 12, a transmission rod 13, a conical hopper 14, a drain pipe 15 and a second filter screen 16, wherein the top end of the chassis 1 is fixedly connected with the support 2, the top of the support 2 is fixedly connected with a separation tank 3, and the top of the separation tank 3 is provided with the thread groove 4;
the filter device comprises a feed inlet 6, a first filter screen 12, a discharge hopper 17, a communicating groove 18, a discharge pipe 19, a filter pipe 20, a third filter screen 21, a separating pipe 22, a valve 23, a bottom cover 24 and a stop block 25, wherein the bottom end of the separating tank 3 is fixedly connected with the discharge hopper 17, the bottom of the discharge hopper 17 is communicated with the discharge pipe 19, and the bottom of the discharge pipe 19 is communicated with the filter pipe 20.
The mixture is conveniently filtered once through the stop block and the first filter screen, filtered articles pass through the first filter screen and fall into the second filter screen, the motor drives the transmission rod and the gear wheel to rotate through the starting motor, the gear wheel drives the pinion connected in a meshed mode to rotate, the pinion drives the transmission rod to rotate, and the transmission rod drives the second filter screen and the conical hopper to rotate, so that centrifugal separation of the mixture is accelerated.
The top of the separation tank 3 is in threaded connection with a top cover 5 through a threaded groove 4, the top of the top cover 5 is provided with a feed inlet 6, the top end of the top cover 5 is provided with a motor 7, one end of the separation tank 3 is internally communicated with a drain pipe 15, and the bottom of the separation tank 3 is provided with a communicating groove 18; the bottom end of the top cover 5 is fixedly connected with a fixed box 10, the inside of the fixed box 10 is rotatably connected with a movable rod 9, and the movable rod 9 is sleeved at the output end of the motor 7; one end of the movable rod 9 is sleeved with a large gear 11, the large gear 11 is meshed with a small gear 8, the small gear 8 is sleeved at one end of a transmission rod 13, and one end of the transmission rod 13 is rotatably connected inside the fixed box 10; a stop block 25 is fixedly connected inside the separation tank 3, a first filter screen 12 is placed at the top end of the stop block 25, a transmission rod 13 is rotatably connected to the center of the first filter screen 12, a second filter screen 16 is fixedly connected to the bottom end of the transmission rod 13, and a conical hopper 14 is fixedly connected to the top end of the second filter screen 16; the inside fixed connection third filter screen 21 of filter tube 20, the bottom intercommunication separator tube 22 of filter tube 20, the internally mounted of separator tube 22 has valve 23, bottom 24 is installed to the bottom of separator tube 22.
When the device is used, firstly, all electrical elements in the device are externally connected with a control switch and a power supply, then a mixture of primary cells and tissues for extracting mesenchymal stem cells after enzymolysis and digestion is added into a separation tank 3 and a top cover 5 through a feed inlet 6, the mixture is conveniently filtered for one time through a baffle 25 and a first filter screen 12, filtered articles pass through the first filter screen 12 and fall onto a second filter screen 16, a motor 7 is started to drive a movable rod 9 and a large gear 11 to rotate, the large gear 11 drives a pinion 8 in meshed connection to rotate, the pinion 8 drives a transmission rod 13 to rotate, the transmission rod 13 drives the second filter screen 16 and a conical hopper 14 to rotate, so that centrifugal separation of the mixture is accelerated, the separated mixture passes through a communicating groove 18, enters a filter pipe 20 through a discharge hopper 17 and a discharge pipe 19, and is fully filtered through a third filter screen 21, the mesenchymal stem cells fall into the separation tube 22, and are conveniently collected and subcultured or further purified by other means through the valve 23 and the bottom cover 24.
The application has the advantages that:
1. the centrifugal separation device is simple in operation, the mixture is conveniently filtered for one time through the stop block and the first filter screen, filtered articles pass through the first filter screen and fall into the second filter screen, the motor drives the movable rod and the large gear to rotate by starting the motor, the large gear drives the pinion in meshed connection to rotate, the pinion drives the transmission rod to rotate, and the transmission rod drives the second filter screen and the conical hopper to rotate, so that the centrifugal separation of the mixture is accelerated;
2. this application is rational in infrastructure, and the mixture after the separation passes the intercommunication groove and in hopper and unloading pipe entering filter tube under through, fully filters the mixture through the third filter screen, and mesenchymal stem cell falls into the separating tube in, through valve and bottom, conveniently collects mesenchymal stem cell and carries out subculture or utilize other means further purification to it.
It is well within the skill of those in the art to implement, without undue experimentation, the present application is not directed to software and process improvements, as they relate to circuits and electronic components and modules.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (6)
1. A mesenchymal stem cell filter separator, which is characterized in that: comprises a chassis (1), a separating tank (3), a separating device and a filtering device;
the separating device comprises a support (2), a thread groove (4), a top cover (5), a motor (7), a pinion (8), a movable rod (9), a fixed box (10), a large gear (11), a first filter screen (12), a transmission rod (13), a conical hopper (14), a drain pipe (15) and a second filter screen (16), wherein the top end of the chassis (1) is fixedly connected with the support (2), the top of the support (2) is fixedly connected with a separating tank (3), and the top of the separating tank (3) is provided with the thread groove (4);
the filter device comprises a feed inlet (6), a first filter screen (12), a discharge hopper (17), a communicating groove (18), a discharge pipe (19), a filter pipe (20), a third filter screen (21), a separating pipe (22), a valve (23), a bottom cover (24) and a stop block (25), the bottom end of the separating tank (3) is fixedly connected with the discharge hopper (17), the bottom of the discharge hopper (17) is communicated with the discharge pipe (19), and the bottom of the discharge pipe (19) is communicated with the filter pipe (20).
2. The mesenchymal stem cell filtration separator according to claim 1, wherein: threaded groove (4) threaded connection top cap (5) are passed through at the top of knockout drum (3), feed inlet (6) have been seted up at the top of top cap (5), motor (7) are installed on the top of top cap (5), inside intercommunication drain pipe (15) of one end of knockout drum (3), intercommunication groove (18) have been seted up to the bottom of knockout drum (3).
3. The mesenchymal stem cell filtration separator according to claim 1, wherein: the bottom fixed connection of top cap (5) is fixed case (10), the inside of fixed case (10) is rotated and is connected movable rod (9), movable rod (9) cup joint the output at motor (7).
4. The mesenchymal stem cell filtration separator according to claim 1, wherein: one end of the movable rod (9) is sleeved with a large gear (11), the large gear (11) is meshed with a small gear (8), the small gear (8) is sleeved at one end of the transmission rod (13), and one end of the transmission rod (13) is rotatably connected to the inside of the fixed box (10).
5. The mesenchymal stem cell filtration separator according to claim 1, wherein: the inside fixed connection dog (25) of knockout drum (3), first filter screen (12) have been placed on the top of dog (25), the center of first filter screen (12) is rotated and is connected with transfer line (13), the bottom fixed connection second filter screen (16) of transfer line (13), the top fixed connection toper fill (14) of second filter screen (16).
6. The mesenchymal stem cell filtration separator according to claim 1, wherein: the inside fixed connection third filter screen (21) of filter tube (20), the bottom intercommunication separator tube (22) of filter tube (20), the internally mounted of separator tube (22) has valve (23), bottom (24) are installed to the bottom of separator tube (22).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121302392.9U CN215328071U (en) | 2021-06-10 | 2021-06-10 | Mesenchymal stem cell filtering separator |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202121302392.9U CN215328071U (en) | 2021-06-10 | 2021-06-10 | Mesenchymal stem cell filtering separator |
Publications (1)
Publication Number | Publication Date |
---|---|
CN215328071U true CN215328071U (en) | 2021-12-28 |
Family
ID=79557104
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202121302392.9U Active CN215328071U (en) | 2021-06-10 | 2021-06-10 | Mesenchymal stem cell filtering separator |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN215328071U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113388506A (en) * | 2021-05-26 | 2021-09-14 | 四川大学华西第二医院 | Automatic mesenchymal stem cell separation device |
-
2021
- 2021-06-10 CN CN202121302392.9U patent/CN215328071U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113388506A (en) * | 2021-05-26 | 2021-09-14 | 四川大学华西第二医院 | Automatic mesenchymal stem cell separation device |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN215328071U (en) | Mesenchymal stem cell filtering separator | |
CN115849534A (en) | Chemical wastewater purification device and use method thereof | |
CN111330346A (en) | Silt separator for wastewater treatment | |
CN211357967U (en) | Oil-water-sand separation treatment device for sewage discharge of oil field combined station | |
CN214004111U (en) | Be used for aquaculture sewage filtering separation device | |
CN112978966A (en) | Oil-water separation device for petrochemical industry | |
CN208512073U (en) | A kind of lithium battery wastewater treatment equipment | |
CN116813050A (en) | Chemical wastewater treatment device with solid-liquid separation function | |
CN111732231A (en) | Integrated high-concentration organic wastewater treatment facility | |
CN216497920U (en) | Solid-liquid separation equipment is used in medicine processing | |
CN110508051A (en) | A kind of water body recovery technology removal device of filtering-pressing integrated | |
CN213965392U (en) | Bio-pharmaceuticals filtration equipment | |
CN205020252U (en) | Processing apparatus is collected to quartz sand tails | |
CN210438688U (en) | Oil-water separation device using demulsifier | |
CN217249557U (en) | Kitchen garbage thick liquids deslagging system | |
CN220878052U (en) | Impurity separation device for methyl methacrylate production | |
CN218307707U (en) | Environment pollution water treatment medicament mixing arrangement | |
CN215712229U (en) | Amoxicillin mother liquor waste water resourceful treatment device | |
CN219620997U (en) | Reverse osmosis membrane water resource circulation purifier | |
CN217809053U (en) | Water environment is administered and is used sewage treatment plant | |
CN217794989U (en) | Solid-liquid separation device for copper sludge treatment | |
CN219942018U (en) | Traditional chinese medicine liquid filtering mechanism | |
CN216358875U (en) | Liquid separating device for chemical synthesis | |
CN219701288U (en) | Convenient calcium carbonate production, filtration, collection and transfer device | |
CN218076848U (en) | Mud discharging mechanism for mud bin of three-phase separator |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant |