CN113388506A - Automatic mesenchymal stem cell separation device - Google Patents

Automatic mesenchymal stem cell separation device Download PDF

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Publication number
CN113388506A
CN113388506A CN202110576792.7A CN202110576792A CN113388506A CN 113388506 A CN113388506 A CN 113388506A CN 202110576792 A CN202110576792 A CN 202110576792A CN 113388506 A CN113388506 A CN 113388506A
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mesenchymal stem
separation
tissue
filtering
main body
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曾珣
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West China Second University Hospital of Sichuan University
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West China Second University Hospital of Sichuan University
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Abstract

The invention discloses an automatic mesenchymal stem cell separation device, relating to the technical field of medical experimental equipment, and the technical scheme is as follows: the device comprises a main body, a filtering device, a tissue separation barrel, a separation cutting knife, a rotating motor, a plurality of derivation pipelines, a first filtering mechanism, a second filtering mechanism, a discharge pipe, a first suction pump, a liquid outlet pipe, a separation collecting device, a second suction pump, a liquid guide pipe, a liquid storage device, a placement platform, a liquid nitrogen storage tank, a circulating delivery pump, a circulating pipeline, a touch control display screen, a power supply device and a single chip microcomputer. When the device is used for separating the mesenchymal stem cells, the device can automatically cut, separate and stir tissues, and filter mixed liquid of the tissue separation in a low-temperature environment, so that pure liquid containing the mesenchymal stem cells is obtained, and the separation of the mesenchymal stem cells is realized; this device degree of automation is high, has reduced staff's work load, and mesenchymal stem cell's separation efficiency is high, and the high quality, mesenchymal stem cell's survival rate is high.

Description

Automatic mesenchymal stem cell separation device
Technical Field
The invention relates to the technical field of medical experimental equipment, in particular to an automatic mesenchymal stem cell separation device.
Background
Mesenchymal Stem Cells (MSCs) are important members of the stem cell family, are derived from early-developing mesoderm, belong to pluripotent stem cells, and are originally found in bone marrow, so that MSCs are increasingly concerned by people due to the characteristics of multidirectional differentiation potential, hematopoietic support, stem cell implantation promotion, immune regulation, self-replication and the like.
Under the specific induction condition in vivo or in vitro, the mesenchymal stem cells can be differentiated into various tissue cells such as fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelium and the like, still have multidirectional differentiation potential after continuous subculture and cryopreservation, can be used as ideal seed cells for repairing tissue and organ injuries caused by aging and pathological changes, and therefore, the mesenchymal stem cells are widely applied to the fields of regenerative biology and regenerative medicine.
Mesenchymal stem cells are mainly derived from bone marrow, fetal tissue (e.g., umbilical cord and placenta), and fat. In recent years, mesenchymal stem cells derived from fetal tissue have been increasingly used because of their simple extraction.
At present, for the acquisition of mesenchymal stem cells in the prior art, the fetal tissue is usually cut up by a traditional mode through manual scissors, so as to separate out the mesenchymal stem cells inside the fetal tissue. In the traditional method for obtaining the mesenchymal stem cells, fetal tissues need to be repeatedly cut by using manual scissors, the cutting operation is complicated, and a large amount of energy and time of medical staff need to be consumed, so that the efficiency of separating the mesenchymal stem cells is low; in addition, in the prior art, the mesenchymal stem cells are separated in a mode of shearing fetal tissues by using manual scissors, so that the mesenchymal stem cells are easily damaged, and the quality of the separated and obtained mesenchymal stem cells is low.
Therefore, the present invention is designed to provide an automatic mesenchymal stem cell isolation apparatus to solve the above problems.
Disclosure of Invention
The invention aims to provide an automatic mesenchymal stem cell separation device, aiming at the technical problems, when the device is used for separating mesenchymal stem cells, the device can automatically cut, separate and stir tissues, and filter mixed liquid of the tissue separation in a low-temperature environment, so that pure liquid containing the mesenchymal stem cells is obtained, and the separation of the mesenchymal stem cells is realized; this device degree of automation is high, has reduced staff's work load, and mesenchymal stem cell's separation efficiency is high, and the high quality, mesenchymal stem cell's survival rate is high.
The technical purpose of the invention is realized by the following technical scheme: an automatic mesenchymal stem cell separation device comprises a main body, wherein a tissue separation device and a filtering device are sequentially arranged in an inner cavity of the main body from top to bottom; the tissue separation device comprises a tissue separation barrel, a separation cutting knife and a rotating motor, wherein the rotating motor is arranged at the outer bottom end of the tissue separation barrel, the separation cutting knife is positioned at the inner bottom end of the tissue separation barrel, and the output end of the rotating motor is connected with the bottom end of the separation cutting knife;
the bottom end of the tissue separation barrel is provided with a plurality of guiding pipelines, one end of each guiding pipeline is communicated with the bottom end of the interior of the tissue separation barrel, and the other end of each guiding pipeline is connected to the top end of the interior of the filtering device; a first filtering mechanism and a second filtering mechanism are sequentially arranged in the filtering device from top to bottom; a discharge pipe is arranged at the bottom end of the filtering device, and one end part of the discharge pipe is positioned at the bottom end in the filtering device;
the bottom end of the main body is provided with a first suction pump close to the filtering device, and the discharge pipe is connected with the suction end of the first suction pump; the outlet end of the first suction pump is connected with a liquid outlet pipe of which the end part is positioned outside the main body; the end part of the liquid outlet pipe is connected with a separation and collection device;
the top end of the main body is provided with a suction pump II, and the suction pump II is connected with a liquid storage device through a liquid guide pipe; the outer side wall of the main body is provided with a placing platform, the bottom end of the placing platform is flush with the bottom end of the main body, and the liquid storage device is positioned on the top surface of the placing platform; a liquid nitrogen storage tank is arranged on the top surface of the placing platform, and a circulating delivery pump is arranged at the top end of the liquid nitrogen storage tank; the output port of the circulating delivery pump is connected with a circulating pipeline, one end of the circulating pipeline is connected with the inlet end of the circulating infusion pump, and the circulating pipeline is arranged on the inner wall of the filtering device;
the side wall of the main body is provided with a touch control display screen and a power supply device, and the touch control display screen is integrated with a single chip microcomputer.
By adopting the technical scheme, when the device in the scheme is used for separating the mesenchymal stem cells, firstly, tissues containing the mesenchymal stem cells are put into the tissue separation barrel from the top end of the tissue separation barrel, and the rotating motor is controlled to work by operating the touch control display screen, so that the separation cutting knife in the tissue separation barrel is driven to rotationally cut and stir the tissues containing the mesenchymal stem cells; in addition, in the process of rotary cutting of the separation cutting knife, the touch control display screen is operated to control the suction pump II to pump the liquid in the liquid storage device into the tissue separation barrel to be mixed with the cut tissue to form mixed liquid containing mesenchymal stem cells; then the mixed liquid enters a filtering device through a guide-out pipeline; the mixed liquid containing the mesenchymal stem cells separated from the tissue separating barrel is conveniently filtered through the filtering device, and tissue residues in the mixed liquid are filtered; the first filtering mechanism in the filtering device is convenient for preliminary filtering of the mixed liquid containing the mesenchymal stem cells, and tissue residues with larger particle diameters are filtered; the mixed liquid is further finely filtered through the second filtering mechanism, so that the mixed liquid containing the mesenchymal stem cells is processed, and pure liquid containing the mesenchymal stem cells is obtained; through the liquid nitrogen storage tank, the circulating delivery pump and the circulating pipeline, a low-temperature environment is conveniently provided for the interior of the filtering device, the survival rate of mesenchymal stem cells in the filtering work is improved, and the separation quality of the mesenchymal stem cells is ensured; the pure liquid containing the mesenchymal stem cells obtained by the filtering device is conveniently pumped into the separation and collection device through the first suction pump for subsequent use of the mesenchymal stem cells; when the device is used for separating the mesenchymal stem cells, the tissue can be automatically cut, separated and stirred, and the mixed liquid of the tissue separation is filtered under the low-temperature environment, so that pure liquid containing the mesenchymal stem cells is obtained, and the separation of the mesenchymal stem cells is realized; this device degree of automation is high, has reduced staff's work load, and mesenchymal stem cell's separation efficiency is high, and the high quality, mesenchymal stem cell's survival rate is high.
The invention is further configured to: the main part and place the platform bottom are equipped with a plurality of support columns, the support column bottom is equipped with pulley mechanism.
By adopting the technical scheme, the main body and the placing platform are conveniently supported through the supporting columns; the pulley mechanism is used for facilitating the movement of the whole separation device.
The invention is further configured to: the main part top surface is equipped with the feeding closing cap, the feeding closing cap can be dismantled with tissue separation bucket and be connected.
Through adopting above-mentioned technical scheme, through the feeding closing cap, be convenient for prevent that external impurity from getting into to organize and lead to the fact the pollution in the separation bucket.
The invention is further configured to: and an antibacterial sealing ring is arranged at the junction of the top end of the tissue separation barrel and the top end of the body.
Through adopting above-mentioned technical scheme, through preventing that the fungus sealing washer is convenient for prevent that external germ from causing the pollution in to the tissue separation bucket.
The invention is further configured to: and a plurality of the leading-out pipelines are provided with electromagnetic valves, and the electromagnetic valves are connected with the single chip microcomputer.
Through adopting above-mentioned technical scheme, the solenoid valve carries out work under singlechip control command's control, is convenient for control the circulation of deriving the pipeline.
The invention is further configured to: the first filtering mechanism is an inverted cone-shaped filtering net; the second filtering mechanism is a rectangular filtering net.
By adopting the technical scheme, the first filtering mechanism is an inverted cone-shaped filtering net, so that preliminary filtering of tissue residues with large particle size is conveniently realized; the second filtering mechanism is a rectangular filter screen, so that the liquid filtered by the first filtering mechanism can be further finely filtered, and the pure liquid containing the mesenchymal stem cells can be conveniently obtained.
The invention is further configured to: the aperture of the inverted conical filter screen is 25-30 μm, and the aperture of the rectangular filter screen is 5-10 μm.
By adopting the technical scheme, the aperture of the inverted cone-shaped filter screen is 25-30 μm, so that large-particle-size tissue residues can be conveniently filtered; the aperture of the rectangular filter screen is 5-10 mu m, so that tissue residues with fine particle sizes can be conveniently filtered, and mesenchymal stem cells can be ensured to pass through.
The invention is further configured to: the bottom surface of the interior of the filtering device is an inclined surface, and one end, close to the discharge pipe, of the bottom surface of the interior of the filtering device is lower than one end, far away from the discharge pipe, of the bottom surface of the interior of the filtering device.
Through adopting above-mentioned technical scheme, filter equipment's inside bottom surface is the inclined plane, and the one end that the inside bottom surface of filter equipment is close to the discharge pipe is less than the one end of keeping away from the discharge pipe, and the pure liquid that contains mesenchymal stem cell that obtains after the convenient filtration can be through one as far as possible all pump-in to separation collection device so that the follow-up use of mesenchymal stem cell.
In conclusion, the invention has the following beneficial effects:
1. when the device is used for separating the mesenchymal stem cells, the tissue can be automatically cut, separated and stirred, and the mixed liquid of the tissue separation is filtered under the low-temperature environment, so that pure liquid containing the mesenchymal stem cells is obtained, and the separation of the mesenchymal stem cells is realized;
2. this device degree of automation is high, has reduced staff's work load, and mesenchymal stem cell's separation efficiency is high, and the high quality, mesenchymal stem cell's survival rate is high.
Drawings
FIG. 1 is a schematic structural diagram in an embodiment of the present invention;
FIG. 2 is a schematic view of the internal structure of a main body in an embodiment of the present invention;
fig. 3 is a block diagram of the structure in the embodiment of the present invention.
In the figure: 1. a main body; 2. a filtration device; 3. a tissue separation barrel; 4. separating the cutting knife; 5. a rotating electric machine; 6. leading out the pipeline; 7. a first filter mechanism; 8. a second filter mechanism; 9. a discharge pipe; 10. a first suction pump; 11. a liquid outlet pipe; 12. a separation and collection device; 13. a second suction pump; 14. a catheter; 15. a liquid storage device; 16. placing a platform; 17. a liquid nitrogen storage tank; 18. a circulating delivery pump; 19. a circulation pipe; 20. a touch control display screen; 21. a power supply device; 22. a single chip microcomputer; 23. a support pillar; 24. a pulley mechanism; 25. feeding and sealing the cover; 26. an antibacterial sealing ring; 27. an electromagnetic valve.
Detailed Description
The present invention is described in further detail below with reference to figures 1-3.
Example (b): an automatic mesenchymal stem cell separation device, as shown in fig. 1, fig. 2 and fig. 3, comprises a main body 1, wherein a tissue separation device and a filter device 2 are sequentially arranged in the inner cavity of the main body 1 from top to bottom. Organize separator including organizing separation bucket 3, separation cutting knife 4 and rotating electrical machines 5, rotating electrical machines 5 sets up in the outside bottom of organizing separation bucket 3, and separation cutting knife 4 is located the inside bottom of organizing separation bucket 3, and rotating electrical machines 5's output is connected with the bottom of separation cutting knife 4.
Organize the bottom of separation bucket 3 and install 2 and derive pipeline 6, derive a tip of pipeline 6 and organize the inside bottom intercommunication of separation bucket 3, another tip that derives pipeline 6 is connected to filter equipment 2's inside top. The inside of filter equipment 2 from top to bottom installs first filtering mechanism 7 and second filtering mechanism 8 in proper order, and first filtering mechanism 7 and the setting of second filtering mechanism 8 interval. The bottom end of the filter device 2 is provided with a discharge pipe 9, and one end part of the discharge pipe 9 is positioned at the bottom end inside the filter device 2.
The lower end of the main body 1 is provided with a first suction pump 10 close to the filter device 2, and the discharge pipe 9 is connected with the suction end of the first suction pump 10. The outlet end of the first suction pump 10 is connected with a liquid outlet pipe 11 with the end part positioned outside the main body 1. The end of the liquid outlet pipe 11 is connected with a separation and collection device 12.
The top end of the main body 1 is provided with a second suction pump 13, and the second suction pump 13 is connected with a liquid storage device 15 through a liquid guide pipe 14. Place the platform 16 is installed to 1 lateral wall of main part, place the bottom and 1 bottom parallel and level of main part of platform 16, and stock solution device 15 is located place the 16 top surfaces of platform. A liquid nitrogen storage tank 17 is arranged on the top surface of the placing platform 16, and a circulating delivery pump 18 is arranged at the top end of the liquid nitrogen storage tank 17. A circulation pipeline 19 is connected to an output port of the circulation transfer pump 18, one end of the circulation pipeline 19 is connected to an inlet end of the circulation transfer pump, and the circulation pipeline 19 is mounted on the inner wall of the filter device 2.
The side wall of the main body 1 is provided with a touch control display screen 20 and a power supply device 21, the touch control display screen 20 is integrated with a single chip microcomputer 22, and the single chip microcomputer 22 is connected with the touch control display screen 20, the rotating motor 5, the first suction pump 10, the second suction pump 13 and the circulating delivery pump 18 and used for sending a control instruction to control the operation of the circulating delivery pump. The touch control display screen 20 is used for inputting control instructions and limiting the working state and working parameters of each working component.
In this embodiment, when the device in this scheme of use carries out mesenchymal stem cell separation work, at first put into the tissue that contains mesenchymal stem cell by the top of tissue separation bucket 3, through in the tissue separation bucket 3, simultaneously, through operation touch control display screen 20, control rotating electrical machines 5 carries out work to the separation cutting knife 4 that drives in the tissue separation bucket 3 carries out rotary cutting and stirring to the tissue that contains mesenchymal stem cell. In addition, in the process of rotating and cutting by the separation cutting knife 4, the second suction pump 13 is controlled by operating the touch control display screen 20 to pump the liquid in the liquid storage device 15 into the tissue separation barrel 3 to be mixed with the cut tissue, so that a mixed liquid containing mesenchymal stem cells is formed. The mixed liquor then enters the filter device 2 through the outlet conduit 6. Through filter equipment 2, be convenient for carry out filtration treatment to the mixed liquid that contains mesenchymal stem cell that separates in tissue separation bucket 3, filter the tissue residue in the mixed liquid. Through the first filtering mechanism 7 in the filtering device 2, the mixed liquid containing the mesenchymal stem cells is conveniently subjected to primary filtration, and tissue residues with large particle diameters are filtered. Through second filtering mechanism 8, be convenient for realize the further fine filtration of mixed liquid to accomplish the processing to the mixed liquid that contains mesenchymal stem cell, obtain the pure liquid that contains mesenchymal stem cell. Through liquid nitrogen holding vessel 17, circulating transfer pump 18 and circulating line 19, be convenient for 2 inside low temperature environment that provide of filter equipment, improve the survival rate of mesenchymal stem cell in the middle of the filtering operation, ensure the quality of mesenchymal stem cell separation. The pure liquid containing the mesenchymal stem cells obtained by the filtering device 2 is conveniently pumped into the separation and collection device 12 by the suction pump I10 for subsequent use of the mesenchymal stem cells. When the device of this scheme is used for mesenchymal stem cell's separation, can cut separation and stirring the tissue automatically to through filtering the mixed liquid of tissue separation under low temperature environment, thereby obtain the pure liquid that contains mesenchymal stem cell, realize mesenchymal stem cell's separation. This device degree of automation is high, has reduced staff's work load, and mesenchymal stem cell's separation efficiency is high, and the high quality, mesenchymal stem cell's survival rate is high.
The bottom fixed welding of main part 1 and place the platform 16 has 4 support columns 23, and support column 23 bottom is fixed mounting respectively has pulley mechanism 24.
In the present embodiment, the support of the main body 1 and the placing platform 16 is facilitated by the support columns 23. The movement of the entire separating apparatus is facilitated by 4 pulley mechanisms 24.
The top surface of the main body 1 is provided with a feeding sealing cover 25, and the feeding sealing cover 25 is detachably and movably connected with the tissue separating barrel 3.
In this embodiment, the feeding cover 25 is provided to prevent foreign substances from entering the tissue separation barrel 3 to cause contamination.
An antibacterial sealing ring 26 is arranged at the junction of the top end of the tissue separation barrel 3 and the top end of the body.
In this embodiment, the bacteria-proof sealing ring 26 is used to prevent the contamination of the tissue separating barrel 3 caused by external bacteria.
The plurality of leading-out pipelines 6 are provided with electromagnetic valves 27, and the electromagnetic valves 27 are connected with the single chip microcomputer 22.
In this embodiment, the electromagnetic valve 27 works under the control of the control instruction of the single chip 22, so as to control the circulation of the outlet pipe 6.
The first filtering mechanism 7 is an inverted cone-shaped filter screen. The second filter mechanism 8 is a rectangular filter screen.
In this embodiment, the first filtering mechanism 7 is an inverted cone-shaped filter screen, which facilitates the preliminary filtering of the tissue residue with large particle size. The second filtering mechanism 8 is a rectangular filter screen, so that the liquid filtered by the first filtering mechanism 7 can be further finely filtered, and the pure liquid containing the mesenchymal stem cells can be conveniently obtained.
The aperture of the inverted cone-shaped filter screen is 25-30 μm, and the aperture of the rectangular filter screen is 5-10 μm.
In the embodiment, the aperture of the inverted cone-shaped filter screen is 25-30 μm, which is convenient for ensuring to filter out large-particle-size tissue residues. The aperture of the rectangular filter screen is 5-10 mu m, so that tissue residues with fine particle sizes can be conveniently filtered, and mesenchymal stem cells can be ensured to pass through.
The inner bottom surface of the filter device 2 is a slope, and one end of the inner bottom surface of the filter device 2 close to the discharge pipe 9 is lower than one end far away from the discharge pipe 9.
In this embodiment, the inner bottom surface of the filtering device 2 is an inclined surface, and one end of the inner bottom surface of the filtering device 2 close to the discharge pipe 9 is lower than one end far away from the discharge pipe 9, so that pure liquid containing mesenchymal stem cells obtained after filtering can be pumped into the separation and collection device 12 through the first suction pump 10 as completely as possible for subsequent use of the mesenchymal stem cells.
The working principle is as follows: when the device in this scheme of use carries out mesenchymal stem cell separation work, at first put into by the top of tissue separation bucket 3 the tissue that contains mesenchymal stem cell, through in the tissue separation bucket 3, simultaneously, through operation touch control display screen 20, control rotating electrical machines 5 carries out work to drive separation cutting knife 4 in the tissue separation bucket 3 and carry out rotary cutting and stirring to the tissue that contains mesenchymal stem cell. In addition, in the process of rotating and cutting by the separation cutting knife 4, the second suction pump 13 is controlled by operating the touch control display screen 20 to pump the liquid in the liquid storage device 15 into the tissue separation barrel 3 to be mixed with the cut tissue, so that a mixed liquid containing mesenchymal stem cells is formed. The mixed liquor then enters the filter device 2 through the outlet conduit 6. Through filter equipment 2, be convenient for carry out filtration treatment to the mixed liquid that contains mesenchymal stem cell that separates in tissue separation bucket 3, filter the tissue residue in the mixed liquid. Through the first filtering mechanism 7 in the filtering device 2, the mixed liquid containing the mesenchymal stem cells is conveniently subjected to primary filtration, and tissue residues with large particle diameters are filtered. Through second filtering mechanism 8, be convenient for realize the further fine filtration of mixed liquid to accomplish the processing to the mixed liquid that contains mesenchymal stem cell, obtain the pure liquid that contains mesenchymal stem cell. Through liquid nitrogen holding vessel 17, circulating transfer pump 18 and circulating line 19, be convenient for 2 inside low temperature environment that provide of filter equipment, improve the survival rate of mesenchymal stem cell in the middle of the filtering operation, ensure the quality of mesenchymal stem cell separation. The pure liquid containing the mesenchymal stem cells obtained by the filtering device 2 is conveniently pumped into the separation and collection device 12 by the suction pump I10 for subsequent use of the mesenchymal stem cells. When the device of this scheme is used for mesenchymal stem cell's separation, can cut separation and stirring the tissue automatically to through filtering the mixed liquid of tissue separation under low temperature environment, thereby obtain the pure liquid that contains mesenchymal stem cell, realize mesenchymal stem cell's separation. This device degree of automation is high, has reduced staff's work load, and mesenchymal stem cell's separation efficiency is high, and the high quality, mesenchymal stem cell's survival rate is high.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims (8)

1. The utility model provides an automatic type mesenchymal stem cell separator, characterized by: comprises a main body (1), wherein the inner cavity of the main body (1) is sequentially provided with a tissue separation device and a filtering device (2) from top to bottom; the tissue separating device comprises a tissue separating barrel (3), a separating cutter (4) and a rotating motor (5), the rotating motor (5) is arranged at the outer bottom end of the tissue separating barrel (3), the separating cutter (4) is positioned at the inner bottom end of the tissue separating barrel (3), and the output end of the rotating motor (5) is connected with the bottom end of the separating cutter (4);
the bottom end of the tissue separation barrel (3) is provided with a plurality of leading-out pipelines (6), one end parts of the leading-out pipelines (6) are communicated with the bottom end inside the tissue separation barrel (3), and the other end parts of the leading-out pipelines (6) are connected to the top end inside the filtering device (2); a first filtering mechanism (7) and a second filtering mechanism (8) are sequentially arranged in the filtering device (2) from top to bottom; a discharge pipe (9) is arranged at the bottom end of the filtering device (2), and one end part of the discharge pipe (9) is positioned at the bottom end inside the filtering device (2);
a first suction pump (10) close to the filtering device (2) is arranged at the bottom end of the main body (1), and the discharge pipe (9) is connected with the suction end of the first suction pump (10); the outlet end of the first suction pump (10) is connected with a liquid outlet pipe (11) the end part of which is positioned outside the main body (1); the end part of the liquid outlet pipe (11) is connected with a separation and collection device (12);
a second suction pump (13) is arranged at the top end of the main body (1), and the second suction pump (13) is connected with a liquid storage device (15) through a liquid guide pipe (14); a placing platform (16) is arranged on the outer side wall of the main body (1), the bottom end of the placing platform (16) is flush with the bottom end of the main body (1), and the liquid storage device (15) is located on the top surface of the placing platform (16); a liquid nitrogen storage tank (17) is arranged on the top surface of the placing platform (16), and a circulating delivery pump (18) is arranged at the top end of the liquid nitrogen storage tank (17); the output port of the circulating delivery pump (18) is connected with a circulating pipeline (19), one end of the circulating pipeline (19) is connected with the inlet end of the circulating infusion pump, and the circulating pipeline (19) is installed on the inner wall of the filtering device (2);
the touch control display screen is characterized in that a touch control display screen (20) and a power supply device (21) are arranged on the side wall of the main body (1), and a single chip microcomputer (22) is integrated on the touch control display screen (20).
2. The device of claim 1, wherein the device comprises: the bottom ends of the main body (1) and the placing platform (16) are provided with a plurality of supporting columns (23), and the bottom ends of the supporting columns (23) are provided with pulley mechanisms (24).
3. The device of claim 1, wherein the device comprises: the top surface of the main body (1) is provided with a feeding sealing cover (25), and the feeding sealing cover (25) is detachably connected with the tissue separating barrel (3).
4. The device of claim 1, wherein the device comprises: an antibacterial sealing ring (26) is arranged at the junction of the top end of the tissue separation barrel (3) and the top end of the body.
5. The device of claim 1, wherein the device comprises: a plurality of lead-out pipelines (6) are provided with electromagnetic valves (27), and the electromagnetic valves (27) are connected with a single chip microcomputer (22).
6. The device of claim 1, wherein the device comprises: the first filtering mechanism (7) is an inverted cone-shaped filter screen; the second filtering mechanism (8) is a rectangular filtering net.
7. The device of claim 6, wherein the device comprises: the aperture of the inverted conical filter screen is 25-30 μm, and the aperture of the rectangular filter screen is 5-10 μm.
8. The device of claim 1, wherein the device comprises: the bottom surface of the interior of the filtering device (2) is an inclined surface, and one end, close to the discharge pipe (9), of the bottom surface of the interior of the filtering device (2) is lower than one end, far away from the discharge pipe (9).
CN202110576792.7A 2021-05-26 2021-05-26 Automatic mesenchymal stem cell separation device Pending CN113388506A (en)

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Application publication date: 20210914