CN215050120U - Hemopoietic stem cell subpackaging instrument - Google Patents
Hemopoietic stem cell subpackaging instrument Download PDFInfo
- Publication number
- CN215050120U CN215050120U CN202120787713.2U CN202120787713U CN215050120U CN 215050120 U CN215050120 U CN 215050120U CN 202120787713 U CN202120787713 U CN 202120787713U CN 215050120 U CN215050120 U CN 215050120U
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- Prior art keywords
- bottle
- stem cell
- liquid
- measuring
- inlet pipe
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- Expired - Fee Related
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- 210000003995 blood forming stem cell Anatomy 0.000 title claims description 5
- 239000007788 liquid Substances 0.000 claims abstract description 67
- 210000000130 stem cell Anatomy 0.000 claims abstract description 35
- 238000007789 sealing Methods 0.000 claims description 19
- 238000004806 packaging method and process Methods 0.000 claims description 16
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 210000003958 hematopoietic stem cell Anatomy 0.000 abstract description 21
- 238000005259 measurement Methods 0.000 abstract description 7
- 238000005138 cryopreservation Methods 0.000 description 7
- 239000011550 stock solution Substances 0.000 description 6
- 239000002390 adhesive tape Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 210000004504 adult stem cell Anatomy 0.000 description 2
- 238000011134 hematopoietic stem cell transplantation Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000014951 hematologic disease Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The hematopoietic stem cell subpackaging instrument comprises a liquid storage bottle, a measuring bottle, a mixing bottle, a first liquid inlet pipe, a second liquid inlet pipe and subpackaging bags, wherein the liquid storage bottle, the measuring bottle, the mixing bottle and the subpackaging bags are sequentially arranged from top to bottom; the first liquid inlet pipe is provided with a first manual switch valve, and the second liquid inlet pipe is provided with a second manual switch valve. During stem cell solution and stem cell protection liquid got into corresponding measuring flask along first feed liquor pipe respectively, utilized the scale in the measuring flask to carry out the direct-viewing measurement, need not empty the measuring flask by hand, improved measuring accuracy. And the measurement of two kinds of liquid can be gone on simultaneously, has improved staff's work efficiency.
Description
Technical Field
The utility model belongs to the partial shipment instrument particularly, relates to a hemopoietic stem cell partial shipment instrument.
Background
Hematopoietic stem cells are adult stem cells in the blood system, are a heterogeneous population, have the ability to self-renew for a long period of time and the potential to differentiate into various types of mature blood cells. The stem cell is the adult stem cell with the longest research history and the most deep, and has important guiding significance for researching various stem cells including tumor stem cells. The mature cells in the blood system have extremely short life, so that the hematopoietic stem cells need to timely supplement each mature cell component of the blood system according to the physiological needs of the organism in a human life, and the hematopoietic stem cell transplantation is the basis for clinically treating blood diseases by utilizing the hematopoietic stem cells. Meanwhile, under the stress states of injury, inflammation and the like, the hematopoietic stem cells play a role in regulating and maintaining the physiological balance of each cellular component of the blood system in vivo.
After hematopoietic stem cells are collected, the collected hematopoietic stem cells need to be subpackaged and then frozen. The cryopreservation of hematopoietic stem cells is one of the key technologies for ensuring the success of hematopoietic stem cell transplantation, so the cryopreservation mode is particularly important. The method is characterized in that the hematopoietic stem cells and the stem cell cryopreservation liquid are stored in an ultra-low temperature refrigerator, before cryopreservation, the hematopoietic stem cells and the stem cell cryopreservation liquid are required to be uniformly mixed according to a certain proportion and then sealed, and different proportions also have different preservation effects and preservation time on the stem cells, so that the hematopoietic stem cells are required to be accurately subpackaged, and meanwhile, the stem cell cryopreservation liquid is required to be accurately injected, so that the accurate proportion of the hematopoietic stem cells and the stem cell cryopreservation liquid is ensured.
At present, workers usually adopt manual subpackage, according to the aseptic operation principle, a syringe is utilized to respectively and accurately inject stem cell frozen stock solution and hematopoietic stem cells, and the work of subpackaging the hematopoietic stem cells can be completed only by the cooperation of the two workers during measurement and subpackage.
Therefore, an accurate hematopoietic stem cell packaging instrument is designed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a hemopoietic stem cell partial shipment instrument of accurate partial shipment is provided.
In order to solve the technical problem, the utility model adopts the following technical scheme:
the hematopoietic stem cell subpackaging instrument comprises a liquid storage bottle, a measuring bottle, a mixing bottle, a first liquid inlet pipe, a second liquid inlet pipe and subpackaging bags, wherein the liquid storage bottle, the measuring bottle, the mixing bottle and the subpackaging bags are sequentially arranged from top to bottom; the first liquid inlet pipe is provided with a first manual switch valve, and the second liquid inlet pipe is provided with a second manual switch valve.
As a further improvement of the above technical solution:
the sub-packaging bag is formed by integrally forming a bag body and a long pipe, the bag body is provided with an opening, and the long pipe is inserted into the opening and is communicated with the bag body. The sub-packaging bag is provided with the long tube, so that whether the bag body is filled with liquid or not can be observed conveniently, and air in the sub-packaging bag can be discharged conveniently.
Still further, the long tube inner wall is equipped with the sealing strip, and the sealing strip includes that the concave type seals the adhesive tape and seals the adhesive tape with the convex type that the concave type matches, and the concave type seals the adhesive tape and sets up on an medial surface of long tube, and the convex type seals the adhesive tape setting on another medial surface of long tube. The concave sealing rubber strip and the convex sealing rubber strip are matched with each other to seal the opening of the long pipe.
Furthermore, the inner wall of the suspended end of the long pipe is provided with a plurality of clamping grooves which are arranged at intervals, the mixed bottle is provided with a joint, the joint is communicated with the mixed bottle, the outer wall of the joint is provided with a plurality of gear rings matched with the clamping grooves, and the long pipe sleeve is arranged on the joint and clamped into the clamping grooves by the gear rings after the joint is arranged. The sub-packaging bag is detachably connected with the mixing bottle, so that the sub-packaging bag does not need to be held by a worker for sub-packaging stem cells, and the workload of the worker is reduced. Meanwhile, in the stem cell subpackaging process, liquid overflows out of the subpackaging bag, so that liquid waste is avoided, and liquid in the subpackaging bag is prevented from being polluted.
The sub-packaging bag is made of soft plastics. Convenient sealed and accomodate.
The hematopoietic stem cell subpackaging instrument also comprises a transparent box, five mounting grooves are arranged in the transparent box, and the liquid storage bottle, the measuring bottle and the mixing bottle are respectively placed in the mounting grooves and fixedly connected with the mounting grooves; the top of the transparent box is provided with two liquid inlets, the transparent box is communicated with the liquid storage bottle through the two liquid inlets, the bottom of the transparent box is provided with a liquid outlet, and the joint extends out of the transparent box from the liquid outlet. Not only ensures sterile operation in the process of subpackaging stem cells, but also is convenient to carry.
Further, the mixing bottle is funnel-shaped.
Compared with the prior art, the utility model has the advantages that:
1. hematopoietic stem cell and stem cell frozen stock liquid are along first feed liquor pipe entering respectively and are corresponded the measuring flask in, utilize the scale in the measuring flask to carry out the direct-viewing measurement, need not empty the measuring flask by hand, improve the measuring accuracy. And the measurement of two kinds of liquid can be gone on simultaneously, has improved staff's work efficiency.
2. The measured liquid is fully mixed in a mixing bottle and then poured into a subpackage bag, so that the stem cell protective solution can protect the stem cell solution more effectively. By using the stem cell subpackaging instrument, the measurement and subpackaging work can be easily finished by one worker.
Drawings
FIG. 1 is a schematic diagram of the stem cell dispensing apparatus.
Fig. 2 is a view showing a state where the adapter is connected to the dispensing bag.
Fig. 3 is an enlarged view of a of fig. 2.
Description of reference numerals:
1. a liquid storage bottle; 2. a first liquid inlet pipe; 3. measuring the bottle; 4. a second liquid inlet pipe; 5. mixing the bottles; 6. a joint; 7. packaging the materials into bags; 8. a transparent box; 9. a sealing strip; 21. a first manual on-off valve; 31. calibration; 41. a second manual switch valve; 61. a ring gear; 71. a long tube; 72. a bag body.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the following detailed description of the present invention, taken in conjunction with the accompanying drawings and the detailed description, is given in a non-limiting manner.
As shown in fig. 1 to 3, the stem cell subpackaging instrument of the embodiment includes two liquid storage bottles 1, two measuring bottles 3, two mixing bottles 5, two first liquid inlet pipes 2, two second liquid inlet pipes 4 and two subpackaging bags 7, wherein the liquid storage bottles 1, the measuring bottles 3, the mixing bottles 5 and the subpackaging bags 7 are sequentially arranged from top to bottom, the two liquid storage bottles 1 and the two measuring bottles 3 are respectively communicated with the measuring bottles 3 through the first liquid inlet pipes 2, the measuring bottles 3 are provided with scales 31, the two measuring bottles 3 are both communicated with the mixing bottles 5 through the second liquid inlet pipes 4, and the subpackaging bags 7 are communicated with the mixing bottles 5; the first liquid inlet pipe 2 is provided with a first manual switch valve 21, and the second liquid inlet pipe 4 is provided with a second manual switch valve 41. The dispensing bag 7 is formed by integrally molding a bag body 72 and a long tube 71, the bag body 72 is provided with an opening, and the long tube 71 is inserted into the opening and is communicated with the bag body 72. The inner wall of the long pipe 71 is provided with a sealing strip 9, the sealing strip 9 comprises a concave sealing rubber strip and a convex sealing rubber strip matched with the concave sealing rubber strip, the concave sealing rubber strip is arranged on one inner side surface of the long pipe 71, and the convex sealing rubber strip is arranged on the other inner side surface of the long pipe 71. The draw-in groove that long tube 71 free end inner wall was equipped with a plurality of intervals and sets up, and mixing bottle 5 is equipped with and connects 6, connects 6 and mixing bottle 5 intercommunication, connects 6 outer walls and is equipped with a plurality of ring gears 61 that match with the draw-in groove, and long tube 71 cover is located and is connected 6 back ring gear 61 cards income draw-in groove. The sub-packaging bag 7 is made of soft plastic.
The hematopoietic stem cell subpackaging instrument further comprises a transparent box 8, five mounting grooves are formed in the transparent box 8, and the liquid storage bottle 1, the measuring bottle 3 and the mixing bottle 5 are respectively placed in the mounting grooves and fixedly connected with the mounting grooves; two liquid inlets are arranged at the top of the transparent box 8, the transparent box 8 is communicated with the liquid storage bottle 1 through the two liquid inlets, a liquid outlet is arranged at the bottom of the transparent box 8, and the joint 6 extends out of the transparent box 8 from the liquid outlet. The mixing flask 5 is funnel-shaped.
For the use of this example, reference is made to the following steps:
firstly, hematopoietic stem cells and stem cell frozen stock solutions are poured into different stock solution bottles 1 respectively. Secondly, two first manual switch valves 21 are opened simultaneously, the stem cells and the stem cell frozen stock solution enter the corresponding measuring bottles 3 along the first liquid inlet pipes 2 respectively, and after the stem cell capacity reaches 20ML and the stem cell frozen stock solution reaches 40ML, the first manual switch valves 21 are closed. Then, the second manual on-off valve 41 is opened, the stem cells and the stem cell frozen stock solution are simultaneously mixed into the mixing bottle 5, and the liquid in the mixing bottle 5 is poured into the dispensing bag 7. Then, the second manual on-off valve 41 is closed, and at the same time, the first manual on-off valve 21 is opened, and the measurement of the volumes of the stem cells and the stem cell culture solution is continued. Finally, the filled portion 7 is sealed by a seal 9.
The hematopoietic stem cell packaging instrument has a simple structure, and each structure has a corresponding specific function.
Claims (7)
1. Hemopoietic stem cell partial shipment instrument, its characterized in that: the liquid storage bottle comprises a liquid storage bottle (1), a measuring bottle (3), a mixing bottle (5), a first liquid inlet pipe (2), a second liquid inlet pipe (4) and a sub-packaging bag (7), wherein the liquid storage bottle (1), the measuring bottle (3), the mixing bottle (5) and the sub-packaging bag (7) are sequentially arranged from top to bottom, the number of the liquid storage bottle (1) and the number of the measuring bottle (3) are two, the two liquid storage bottles (1) are respectively communicated with the measuring bottle (3) through the first liquid inlet pipe (2), scales (31) are arranged on the measuring bottle (3), the two measuring bottles (3) are both communicated with the mixing bottle (5) through the second liquid inlet pipe (4), and the sub-packaging bag (7) is communicated with the mixing bottle (5); the first liquid inlet pipe (2) is provided with a first manual switch valve (21), and the second liquid inlet pipe (4) is provided with a second manual switch valve (41).
2. A stem cell dispensing apparatus as defined in claim 1, wherein: the sub-packaging bag (7) is formed by integrally forming a bag body (72) and a long pipe (71), the bag body (72) is provided with an opening, and the long pipe (71) is inserted into the opening and is communicated with the bag body (72).
3. A stem cell dispensing apparatus as defined in claim 2, wherein: the inner wall of the long pipe (71) is provided with a sealing strip (9), the sealing strip (9) comprises a concave sealing rubber strip and a convex sealing rubber strip matched with the concave sealing rubber strip, the concave sealing rubber strip is arranged on one inner side surface of the long pipe (71), and the convex sealing rubber strip is arranged on the other inner side surface of the long pipe (71).
4. A stem cell dispensing apparatus as defined in claim 3, wherein: long tube (71) free end inner wall is equipped with the draw-in groove that a plurality of intervals set up, and mixing bottle (5) are equipped with joint (6), connect (6) and mixing bottle (5) intercommunication, connect (6) outer wall to be equipped with a plurality of ring gear (61) that match with the draw-in groove, and long tube (71) cover is located and is connected (6) back ring gear (61) card income draw-in groove.
5. A stem cell dispensing apparatus as defined in claim 4, wherein: the sub-packaging bag (7) is made of soft plastics.
6. A stem cell dispensing apparatus as defined in claim 1, wherein: the liquid storage bottle (1), the measuring bottle (3) and the mixing bottle (5) are respectively placed in the mounting grooves and fixedly connected with the mounting grooves; two liquid inlets are arranged at the top of the transparent box (8), the transparent box (8) is communicated with the liquid storage bottle (1) through the two liquid inlets, a liquid outlet is arranged at the bottom of the transparent box (8), and the joint (6) extends out of the transparent box (8) from the liquid outlet.
7. A stem cell dispensing apparatus as defined in claim 6, wherein: the mixing bottle (5) is funnel-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120787713.2U CN215050120U (en) | 2021-04-13 | 2021-04-13 | Hemopoietic stem cell subpackaging instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120787713.2U CN215050120U (en) | 2021-04-13 | 2021-04-13 | Hemopoietic stem cell subpackaging instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215050120U true CN215050120U (en) | 2021-12-07 |
Family
ID=79105694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120787713.2U Expired - Fee Related CN215050120U (en) | 2021-04-13 | 2021-04-13 | Hemopoietic stem cell subpackaging instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215050120U (en) |
-
2021
- 2021-04-13 CN CN202120787713.2U patent/CN215050120U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211207 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |