CN214622434U

CN214622434U - Electrochemical biosensor

Info

Publication number: CN214622434U
Application number: CN202120162366.4U
Authority: CN
Inventors: 周林福; 侯超; 秦玉
Original assignee: Nanjing Jingjie Biotechnology Co ltd; Jiangsu Province Hospital First Affiliated Hospital With Nanjing Medical University
Current assignee: Nanjing Jingjie Biotechnology Co ltd; Jiangsu Province Hospital First Affiliated Hospital With Nanjing Medical University
Priority date: 2021-01-21
Filing date: 2021-01-21
Publication date: 2021-11-05
Anticipated expiration: 2031-01-21

Abstract

The utility model discloses an electrochemistry biosensor belongs to medical equipment technical field. The sensor includes: the electrode layer comprises a red blood cell calibration working electrode, a red blood cell calibration counter electrode, a working electrode, a counter electrode and a sample introduction detection electrode; and the middle interlayer is positioned on the electrode layer and comprises a single-sided adhesive layer and a hydrophilic film layer covering the single-sided adhesive layer. A reagent window is formed in the single-side adhesive layer, so that the electrode part is covered with a reaction reagent; the reagent window is used for limiting the area of the reaction reagent; the hydrophilic film layer is provided with an air vent on the upper part of the reagent window; and a label layer on the electrode layer, thereby constituting a complete electrochemical biosensor. The utility model has the advantages of simple manufacturing process, easy batch production and quick and accurate detection.

Description

Electrochemical biosensor

Technical Field

The utility model belongs to the technical field of medical equipment, concretely relates to an electrochemistry biosensor for among metabolite detection equipment.

Background

In recent years, Point-of-Care Testing (Point-of-Care Testing) products are widely used, and are mainly characterized by rapid detection, simple and convenient operation, small size, portability, stable result and comparability. The user utilizes the electrochemical biosensor of disposable formula to detect at home, just can obtain analyte concentration at that time, helps the user in time to know self health and adjust the dietary structure of oneself in view of the above, or gets to the hospital and lets the doctor adjust the dosage of using medicine according to the data of daily control, for example, blood lactic acid monitoring can indicate tissue oxygen deficiency or metabolic disorder, judges patient's prognosis, detects recovery treatment. Therefore, it is important to design and develop disposable electrochemical biosensors with easy mass production and low cost.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an electrochemistry biosensor, it has simple, the easy batch production of manufacturing process, detects quick accurate advantage.

The utility model provides a technical scheme that its technical problem adopted is:

an electrochemical biosensor, comprising:

the electrode layer (1), the electrode layer (1) includes red blood cell calibration working electrode (11), red blood cell calibration counter electrode (12), working electrode (13), counter electrode (14) and sample injection detection electrode (15);

the middle separation layer (2) is positioned on the electrode layer (1), and the middle separation layer (2) comprises a single-sided adhesive layer (21) and a hydrophilic film layer (23) covering the single-sided adhesive layer; a reagent window (22) is formed in the single-sided adhesive layer (21), so that the electrode part is covered with the reaction reagent (16); a reagent window (22) to define a region of the reaction reagent (16); the hydrophilic film layer (23) is provided with an exhaust hole (24) on the upper part of the reagent window (22); a label layer (3) on the electrode layer (1), thereby constituting a complete electrochemical biosensor (4).

The middle interlayer (2) comprises a single-sided adhesive layer (21) and a hydrophilic film layer (23) covering the single-sided adhesive layer, wherein the single-sided adhesive layer (21) is a pressure-sensitive adhesive with a certain thickness, so that the middle interlayer (2) is adhered to the electrode layer (1); the hydrophilic film layer (23) is a transparent high polymer sheet subjected to hydrophilic treatment and covers the single-side glue layer (21). The electrode layer (1) is a metal thin layer attached to a high polymer material, and a corresponding electrode pattern is realized through mask sputtering/electroplating or laser etching; the material of the metal thin layer can be gold, platinum, palladium and the like or alloy thereof.

The reactive agent covers at least the electrode system of the working electrode and the counter electrode.

The reactive reagent located on the reagent window (22) includes an enzyme and an electron mediator.

The enzyme is glucose oxidase, glucose dehydrogenase, lactate oxidase, lactate dehydrogenase, beta-hydroxybutyrate dehydrogenase, urate oxidase, cholesterol oxidase, creatinine hydrolase, etc.

The electron mediator can be a ruthenium complex, a ferrocene derivative, a phenazine derivative, or the like.

Further, the reaction reagent also comprises a buffer solution selected from a biological buffer solution or a zwitterionic buffer solution. Preferably, Good's buffer solution, organic acid buffer solution or phosphate buffer solution is used. More preferably, the buffer solution is Good's buffer solution, and may be one or more of ACES, TES, MES.

The reagents may also include enzyme stabilizers, which are readily known to those of ordinary skill in the art as: sugars, such as sucrose; polyols, such as sorbitol; amino acids such as sodium glutamate; proteins, such as bovine serum albumin; and betaine derivatives disclosed in patent CN 107941880B.

The reactive agent also includes at least one surfactant, which may be ionic and nonionic. The surfactant enables the liquid reaction reagent to be uniformly distributed and spread on the surface of the electrode, and simultaneously improves the hydrophilicity of the dried reaction reagent, thereby improving the sample introduction rate of the detected blood sample.

The reactive agent further comprises a high polymer. Polymers used in the context of the present invention are well known in the art, such as methylcellulose.

The detection sample of the electrochemical biosensor can be a blood sample such as whole blood, plasma, serum and the like, and can also be other types of samples such as urine, sweat, tears, interstitial fluid and the like.

A preparation method of an electrochemical biosensor comprises the following specific preparation processes: the metal thin layer attached on the high polymer material realizes the corresponding electrode pattern through mask sputtering/electroplating or laser etching; the metal thin layer can be gold, platinum, palladium and the like or alloy thereof, and the thickness of the thin layer can be from several nanometers to several hundred nanometers. Then the liquid reaction reagent is configured to a reagent window area in a liquid spotting mode; drying to remove solvent water in the liquid reaction reagent, thereby obtaining a dried reagent layer with uniform thickness; and finally, adhering a middle interlayer to obtain the electrochemical biosensor.

The test method of the electrochemical biosensor of the utility model is as follows:

after the sample is sucked by capillary action, an excitation voltage of 100mV-600mV, preferably 150mV-350mV, is applied between the working electrode and the counter electrode, and the current value of the sample is acquired at 3 seconds-30 seconds by a chronoamperometry method, and then converted into the metabolite test value of the sample by a calibration curve.

The utility model has the advantages as follows:

1. the utility model discloses an electrochemistry biosensor's structural design is reasonable, and reagent layer covers evenly, and it is unobstructed to exhaust during the detection, advances kind fast, is favorable to improving electrochemistry biosensor's the degree of accuracy and precision.

2. The utility model discloses an electrochemistry biosensor, simple process, a little liquid can accomplish the configuration of reaction reagent on the electrode. The raw materials of the reaction reagent are easy to obtain, the cost is low, the industrial production is facilitated, and the practicability is high.

3. The utility model discloses an electrochemistry biosensor's check-out time is short, can acquire data 3 seconds-30 seconds, can reach the purpose of instant detection.

Drawings

FIG. 1 is a schematic structural view of an electrochemical biosensor according to the present invention.

Fig. 2 is a calibration curve of the lactate electrochemical biosensor of the present invention.

In the figure: 1-an electrode layer; 2-a middle barrier layer; 3-a label layer; 4-an electrochemical biosensor; 11-red blood cell calibration working electrode; 12-red blood cell calibration counter electrode; 13-a working electrode; 14-an electrode; 15-sample introduction detection electrode; 16-a reactive agent; 21-single-sided adhesive layer; 22-reagent window; 23-a hydrophilic membrane layer; 24-vent hole.

Detailed Description

The technical solution of the present invention is further described in detail by the following specific examples, but it should be pointed out that the following examples are only used for describing the content of the present invention, and do not limit the scope of the present invention.

As shown in fig. 1, the structure of the electrochemical biosensor for lactic acid of this embodiment includes:

the middle separation layer (2) is positioned on the electrode layer (1), and the middle separation layer (2) comprises a single-sided adhesive layer (21) and a hydrophilic film layer (23) covering the single-sided adhesive layer; a reagent window (22) is formed in the single-sided adhesive layer (21), so that the electrode part is covered with the reaction reagent (16); a reagent window (22) to define a region of the reaction reagent (16); the hydrophilic film layer (23) is provided with an air vent (24) on the reagent window (22);

a label layer (3) on the electrode layer (1), thereby forming a complete electrochemical biosensor (4)

In the electrochemical biosensor, an electrode layer (1) is a metal thin layer attached to a high polymer material, and a corresponding electrode pattern is realized through laser etching; the electrode material may be gold. The electrode pattern was laser etched by vacuum sputtering a 30nm gold layer on a polyethylene terephthalate (PET) sheet.

The middle isolation layer (2) comprises a single-side adhesive layer (21) and a hydrophilic film layer (23) covering the single-side adhesive layer, wherein the single-side adhesive layer (21) is a pressure-sensitive adhesive with a certain thickness, preferably, the single-side adhesive layer is made of polyacrylic acid. The middle interlayer (2) is adhered on the electrode layer (1); the hydrophilic film layer (23) is a transparent polyethylene terephthalate sheet which is subjected to hydrophilic treatment and covers the single-side adhesive layer.

The preparation method of the electrochemical sensor for lactic acid of the embodiment is as follows:

first, a liquid reaction reagent was prepared according to Table 1, wherein lactate oxidase (type: T-47,42U/mg) was purchased from Asahi chemical (ASAHI KASEI). Then, liquid reaction reagent is configured in the area of a reagent window (22) in a point-to-point liquid mode, and the loading amount of each lactic acid electrochemical sensor is 1.0 mg. And sequentially carrying out heat treatment and drying in a drying tunnel section at 45 ℃ and a drying tunnel section at 70 ℃ for 20 minutes and 10 minutes. After sticking, pressing and cutting, the finished product of the lactic acid electrochemical sensor is stored in a closed plastic cylinder with a molecular sieve drying agent.

TABLE 1 liquid reaction reagent composition

It is well known to those skilled in the art that in order to determine the amount of an analyte, a corresponding electrochemical signal is obtained; more specifically, the lactate electrochemical sensor of the chronoamperometry method obtains the corresponding current signal of a blood sample with unknown lactate concentration, and then converts the signal into the lactate concentration value of the blood sample through a calibration equation. How such calibration equations can be established and used is well known to those of ordinary skill in the art.

The experimental process comprises the following steps: the cylindrical lactic acid electrochemical biosensor is placed for more than 30 minutes at the room temperature of 23 +/-2 ℃. The blood samples were as follows: adjusting the hematocrit ratio to 42% +/-2%; the oxygen partial pressure is controlled to be 65mmHg +/-5 mmHg; 12 whole blood lactic acid samples were prepared in the concentration range of 0-35mmol/L, and venous whole blood samples were assigned a value by the YSI2500 glucose/lactic acid analyzer from Jinquan corporation, each sample tested for 5 lactate electrochemical biosensors. In this example, the current value at 10 seconds was read using an excitation voltage of 300mV, and the current data in table 2 was obtained. The average value of the test current in each concentration section is used as the abscissa, and the YSI whole blood lactate value is used as the ordinate to perform linear fitting, and a calibration curve is established as shown in FIG. 2.

Table 2 calibration curve test current data for lactate electrochemical sensors

As can be seen from FIG. 2, the square of the correlation coefficient (R) of the linear fit was established using the YSI2500 glucose/lactate analyzer whole blood lactate value and the average value of the test current to establish a calibration curve²) Greater than 0.99, with good fitting.

The above description is only a preferred embodiment of the present invention, and does not limit the scope of the present invention. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. An electrochemical biosensor, comprising:

the middle separation layer (2) is positioned on the electrode layer (1), and the middle separation layer (2) comprises a single-sided adhesive layer (21) and a hydrophilic film layer (23) covering the single-sided adhesive layer; a reagent window (22) is formed in the single-sided adhesive layer (21), so that the electrode part is covered with the reaction reagent (16); a reagent window (22) to define a region of the reaction reagent (16); the hydrophilic film layer (23) is provided with an exhaust hole (24) on the upper part of the reagent window (22);

a label layer (3) on the electrode layer (1), thereby constituting a complete electrochemical biosensor (4).

2. An electrochemical biosensor according to claim 1, wherein the single-sided adhesive layer (21) is a pressure sensitive adhesive having a thickness such that the spacer layer (2) is adhered to the electrode layer (1); the hydrophilic film layer (23) is a transparent high polymer sheet subjected to hydrophilic treatment and covers the single-side glue layer (21).

3. An electrochemical biosensor according to any of claims 1-2, wherein the electrode layer (1) is a thin metal layer attached to a polymer material, and the corresponding electrode pattern is realized by mask sputtering/electroplating or laser etching.

4. An electrochemical biosensor as claimed in claim 3, wherein the material of the metal thin layer is gold, platinum, palladium, etc. or alloy thereof.

5. An electrochemical biosensor according to any one of claims 1-2, 4, wherein the reactant covers at least the electrode system of the working electrode and the counter electrode.