CN108896635A - A kind of beta-hydroxybutyric acid electrochemical sensor - Google Patents
A kind of beta-hydroxybutyric acid electrochemical sensor Download PDFInfo
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Abstract
The invention discloses a kind of beta-hydroxybutyric acid electrochemical sensors, belong to technical field of medical equipment.The sensor includes：Substrate layer, there are sample introduction notches for the end of substrate layer；Electrode layer on the substrate layer, the electrode layer include working electrode, to electrode, booting electrode and conducting wire；Insulating layer on the electrode layer is provided with reagent window, the exposure working electrode and the working portion to electrode at the insulating layer；Reagent window is to defined reaction reagent；Middle interlayer on the insulating layer, there are hydrophilic window and exhaust window, hydrophilic windows and exhaust window and the reagent window to constitute sample introduction space for the middle interlayer.The present invention has the advantages that reasonable in design, reagent compatibility optimization, accuracy in detection are high.
Description
Technical field
The invention belongs to technical field of medical equipment, and in particular to a kind of β in whole blood ketoboidies detection device-hydroxyl fourth
Sour electrochemical sensor.
Background technique
Ketoboidies is the product of fatty acid catabolism, including three kinds of ingredients：Beta-hydroxybutyric acid, acetoacetate and acetone, wherein
Beta-hydroxybutyric acid is main component, accounts for about the 78% of ketoboidies total amount.Liver generates ketoboidies, and extrahepatic tissue can be using ketoboidies as energy
Source contains only a small amount of ketoboidies under normal circumstances in blood, but in some cases as obstacle, body occur for carbohydrate metabolism
Lipid mobilization enhancing, it is more than the ability that extrahepatic tissue utilizes ketoboidies that liver, which generates ketoboidies amount, causes the accumulation of ketoboidies in blood, can lead
Ketoacidosis is caused, therefore the concentration of beta-hydroxybutyric acid is particularly important in detection blood in time.
The 8th edition global diabetes general view issued according to international diabetes association (IDF) in 2017, the data display whole world
Just there is 1 diabetic in every 11 adults, China's diabetic's number reaches 1.144 hundred million, occupies the whole world first.Diabetes
Ketoacidosis (DKA) is a kind of common Diabetic Acute complication.Diabetic suggests in American Diabetes Association (ADA)
It should all check that beta-hydroxybutyric acid is dense in blood when acute illness, stress reaction, pregnancy and blood glucose till are more than 13.4mmol/L
Degree.Therefore, the concentration that a kind of simple and rapid method is used to detect beta-hydroxybutyric acid is established, prevention, diagnosis to ketosis in clinic
And monitoring has great significance.
In recent years, the disposable electrochemical sensor prepared by screen printing technique obtains in detection field (POCT) immediately
To extensive use.The meaning of POCT can be understood in terms of two：Spatially, the detection carried out at one's side in patient, i.e. " inspection by bed
It surveys "；On time, testing result can be quickly obtained, i.e. " detection immediately ".POCT be mainly characterized by quickly detect, it is easy to operate,
Miniature portable, result are stable, are comparable etc..Patient is detected using disposable biological sensor at home, so that it may
The analyte concentration of patient at that time is obtained, patient is helped to understand the physical condition of itself in time and adjust the diet knot of oneself accordingly
Structure, or allow doctor to be adjusted according to the data of daily monitoring to dosage to hospital.Therefore in the daily monitoring of disease this
The accuracy of kind disposable biosensor is particularly important, while must be susceptible to produce in batches, cheap.
Patent CN101900734B discloses a kind of semi-quantitative diagnosis test paper using development process detection beta-hydroxybutyric acid concentration.
Patent CN102435749B discloses a kind of liquid reagent box of enzymatic cycling assay for detecting beta-hydroxybutyric acid concentration, detects dominant wavelength
505nm.Patent CN104730230B discloses a kind of beta-hydroxybutyric acid enzyme process detection kit, detects dominant wavelength 340nm.Patent
CN2788184Y discloses a kind of beta-hydroxybutyric acid biological testing bar of electrochemical process, and the reaction reagent of reaction zone includes：β-hydroxyl
Butyryl dehydrogenase >=1.2IU, diaphorase >=1U, the potassium ferricyanide：0.1mg, other salts：64%.Patent
CN102636539A discloses a kind of detection beta-hydroxybutyric acid electrochemistry test paper, and reagent enzyme layer includes hydroxybutyrate dehydrogenase, reproducibility
Coenzyme, the potassium ferricyanide, chitosan etc..
In the above technology path, it is suitable for biochemical analysis using the detection kit accurate quantitative analysis beta-hydroxybutyric acid of optical method
Instrument, it is difficult to meet the needs of portable POCT product.In addition, the normal physiologic concentrations of beta-hydroxybutyric acid are (i.e. lower than 0.6mmol/L
7.56mg/dL), have the tendency that ketoacidosis more than the concentration, therefore it is required that detection kit has lower linear lower limit,
To guarantee the accuracy of extremely low concentration testing result.In addition, this field researcher is apparent from, with electrochemical sensor effective day
Phase closes on, physical and chemical changes (such as the gradually forfeiture of enzyme activity, the electron mediator iron cyanogen of component in the reagent layer of dry type reagent
Change potassium be reduced) will lead to electrochemical sensor accuracy deterioration.
Summary of the invention
In order to overcome shortcoming and deficiency existing in the prior art, the purpose of the present invention is to provide a kind of beta-hydroxybutyric acid electricity
Chemical sensor has the advantages that reasonable in design, reagent compatibility optimization, accuracy in detection are high.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of beta-hydroxybutyric acid electrochemical sensor, including：
Substrate layer (1), there are semicircular sample introduction notches (11) for front end；Electrode layer on the substrate layer (1)
(2), the electrode layer (2) includes working electrode (21) and to electrode (22) and booting electrode (23) and conducting wire (24)；
Insulating layer (3) on the electrode layer (2) is provided with reagent window (31) at the insulating layer (3), exposure work
Make electrode (21) and the working portion to electrode (22)；Reagent window (31) is to defined reaction reagent, the reaction reagent of liquid
Drop in this region, restriction means that liquid reactions reagent can be made to be difficult to except overflow area；
The middle interlayer (4) being pasted on the insulating layer (3), there are hydrophilic windows (41) and exhaust for the middle interlayer (4)
Window (42)；
Hydrophilic window (41) and exhaust window (42) and reagent window (31) constitute sample introduction space.
Further, the shape of the sample introduction notch (11) is arc, and sample introduction notch (11) is arranged in substrate layer (1) on pair
Working electrode described in Ying Yu (21) and one end to electrode (22).
Further, the hydrophilic window (41) and exhaust window (42), reagent window (31) and sample introduction notch (11) structure
At the accommodation space of blood sample sample introduction.
Further, has pressure sensitive adhesive on the middle interlayer (4), so that middle interlayer (4) is pasted on insulating layer (3)；
The hydrophilic window (41) is the PET by hydrophilic treated, and there is no pressure sensitive adhesives；The exhaust window (42) open wide setting with it is outer
The perforation of boundary's air.
Further, substrate layer (1) material is polyethylene terephthalate；The working electrode (21) and to electricity
The material of pole (22) is carbon；The material of booting electrode (23) and conducting wire (24) is silver；The material of the insulating layer (3) is thin
Water-based material；The middle interlayer (4) is polyacrylic acid one side glue.
Further, the reaction reagent on reagent window (31) includes:(a) enzyme；(b) enzymatic protective reagent；(c) electronics is situated between
Body composition；(d) buffer solution；(e) at least one surfactant；(f) at least one cellulose family high polymer.
The enzyme includes beta-hydroxybutyric dehydrogenase, diaphorase, coenzyme (nicotinamide adenine dinucleotide).
Those of ordinary skill in the art's enzyme protection easy to know, which determines agent, to be had：Carbohydrate, such as trehalose；Polyalcohols, such as mountain
Pears alcohol；Amino acids, such as sodium glutamate；Protide, such as silk-fibroin.Preferably, the enzymatic protective reagent is carbohydrate, using sea
Algae sugar.
The electron mediator applied in the context of the present invention be it is well known in the art, the electron mediator composition includes chlorination
Six ammino rutheniums and azophenlyene, azophenlyene are 5- toluphenazine Methylsulfate, 5- ethyl phenazine ethyl-sulfate, 1- methoxyl group -5- methyl pheno
One of piperazine Methylsulfate is a variety of；Preferably, the electron mediator composition is hexaammine ruthenium chloride and 1- methoxyl group -5-
Toluphenazine Methylsulfate.
The buffer solution is selected from biological buffer or amphoteric ion buffer solution；Preferably, it is buffered using Good's molten
Liquid, organic acid buffer solution or phosphate buffer solution；It is furthermore preferred that the buffer solution is one of ACES, TES, MES
Or it is a variety of.
The reaction reagent further includes at least one surfactant, can be nonionic surfactant, it is preferred that is adopted
With X-100。X-100 causes the reaction reagent of the liquid after liquid equably to configure to spread over electricity
Pole surface, while improving the hydrophily of dry reaction reagent, to improve beta-hydroxybutyric acid electrochemical sensor in detection blood
Sample rate when sample.
The reaction reagent further includes at least one cellulose family high polymer, it is preferred that is hydroxymethyl cellulose.High polymer
The effect that bracket is played in reaction reagent, facilitates the dispersion and stabilization of enzyme, while it is good that dry reaction reagent is formed a film
Good, adhesive force is strong.
Specifically, a kind of reaction reagent of beta-hydroxybutyric acid electrochemical sensor of the invention includes：Beta-hydroxybutyric dehydrogenase
Concentration be 0.5-5wt.% (by enzymatic activity for 200u/mg in terms of), the concentration of diaphorase is that 0.5-5wt.% (is with enzymatic activity
200u/mg meter), the concentration of coenzyme (nicotinamide adenine dinucleotide) is 0.5-5wt.%, trehalose 0.1-1wt.%, chlorination
The concentration of six ammino rutheniums is 0.5-5wt.%, and the concentration of 1- methoxyl group -5- toluphenazine Methylsulfate is 0.005-
The concentration of 0.05wt.%, TES buffer solution (concentration of TES is 0.1-1M, pH 6.5-7.5 in buffer solution) is 10-
50wt.%,The concentration of X-100 is 0.3-3wt.%, and the concentration of hydroxymethyl cellulose is 0.25-2.5wt.%.
A kind of preparation method of beta-hydroxybutyric acid electrochemical sensor, it is specific as follows：
It is successively inked with carbon oil in a manner of silk-screen printing on the substrate layer of polyethylene terephthalate (PET) material
Brush working electrode and to electrode, prints booting electrode and conducting wire with silver ink；It is exhausted using the dielectric ink printing of hydrophobic material
Edge layer.The reaction reagent that liquid is configured according to above-mentioned reaction reagent composition, is sufficiently stirred and makes it dissolve dispersion, is formed uniform
Solution；Then the reaction reagent of above-mentioned liquid is configured on the reagent window in insulating layer by way of liquid；It removes again
Solvent in the reaction reagent of liquid, to obtain the uniform reagent layer of dry thickness；Interlayer in finally pasting, thus finally
Obtain a kind of finished product of beta-hydroxybutyric acid electrochemical sensor.
The test method of beta-hydroxybutyric acid electrochemical sensor of the invention is as follows：
Through capillary action after the completion of draw blood sample, working electrode and between electrode apply 300mv work
Voltage is obtained the current value of blood sample in 10s, is then converted into blood sample by Calibration equation using chronoamperometry
This beta-hydroxybutyric acid concentration value.
Beneficial effects of the present invention are as follows：
1, a kind of beta-hydroxybutyric acid electrochemical sensor of the invention is reasonable in design, and by insulating layer, there are window limits
Determine reaction reagent, so that reagent layer covering is uniformly, smooth gas discharge when detection, sample introduction is quick, is conducive to improve beta-hydroxybutyric acid electrification
Learn the accuracy and precision of sensor.
2, the reaction reagent compatibility in a kind of beta-hydroxybutyric acid electrochemical sensor of the invention is reasonable, and thermal stability is good, has
Effect is avoided as validity date closes on, the accuracy of sensor deterioration, to ensure that testing result, especially extremely low concentration
Sample (refers to the blood sample of the beta-hydroxybutyric acid concentration of detectable 0-3mg/dL, routinely examines lower than in background technique in embodiment
The Monitoring lower-cut 7.56mg/dL of survey) testing result accuracy, so practicability is stronger.
3, a kind of structurally reasonable, the simple process of beta-hydroxybutyric acid electrochemical sensor of the invention, reaction reagent raw material are easy
, it is at low cost, be conducive to industrialized production.
4, a kind of detection time of beta-hydroxybutyric acid electrochemical sensor of the invention is short, and 5-20s can obtain data, can be with
Complete the purpose detected immediately.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of beta-hydroxybutyric acid electrochemical sensor of the invention；
Fig. 2 is the linear graph of beta-hydroxybutyric acid electrochemical sensor accuracy of the present invention；
Fig. 3 is 65 DEG C of beta-hydroxybutyric acid electrochemical sensor accelerated ageing 3 days of the present invention, 7 days, relative deviation signal in 10 days
Figure；
Wherein, 1- substrate layer, 11- sample introduction notch；2- electrode layer, 21- working electrode, 22- is to electrode, 23 booting electrodes,
24- conducting wire；3- insulating layer, 31- reagent window；Interlayer in 4-, the hydrophilic window of 41-, 42- exhaust window；5- beta-hydroxybutyric acid electrification
Learn sensor.
Specific embodiment
Technical solutions according to the invention are further described in detail below by specific embodiment, but it is necessary to
It points out that following embodiment is served only for the description to summary of the invention, does not constitute a limitation on the scope of protection of the present invention.
As shown in Figure 1, the structure of the beta-hydroxybutyric acid electrochemical sensor of the present embodiment includes：Substrate layer 1, there are half for front end
Circular sample introduction notch 11；Electrode layer 2 on substrate layer 1, electrode layer 2 is including working electrode 21 and to electrode 22 and opens
Machine electrode 23 and conducting wire 24；Insulating layer 3 on electrode layer 2 is provided with reagent window 31, exposure working electrode at insulating layer 3
21 and to the working portion of electrode 22；Reagent window 31 is to defined reaction reagent；The middle interlayer 4 being pasted on insulating layer 3, in
There are hydrophilic window 41 and exhaust windows 42 for interlayer 4；Hydrophilic window 41 and exhaust window 42 and reagent window 31 constitute sample introduction
Space.
Wherein, 1 material of substrate layer is polyethylene terephthalate；Working electrode 21 and be carbon to the material of electrode 22；
The material of booting electrode 23 and conducting wire 24 is silver；The material of insulating layer 3 is hydrophobic material；Middle interlayer 4 is polyacrylic acid single side
Glue.
The preparation method of the beta-hydroxybutyric acid electrochemical sensor of the present embodiment is as follows：
The reaction reagent for completing liquid is deployed first, in accordance with table 1, wherein beta-hydroxybutyric dehydrogenase (model：HBD-301,
167U/mg), diaphorase (model：DAD-311,841U/mg) and coenzyme (β-NAD+) it is purchased from Japanese Japan's spinning
(TOYOBO).Then the reaction reagent of liquid is configured to reagent window 31, every beta-hydroxybutyric acid electrification by way of liquid
The load capacity of sensor 5 is learned in 1.3mg-1.7mg.In 30 DEG C of -40 DEG C of drying tunnel sections, 40 DEG C of -50 DEG C of drying tunnel sections, 50 DEG C of -60 DEG C of bakings
It is successively heat-treated in road section 10 minutes.After cutting, beta-hydroxybutyric acid electrochemical sensor 5 is deposited by pressing interlayer 4 in fitting
It is put in the closed plastic cylinder with molecular sieve desiccant.
Table 1：The reaction reagent of liquid forms
It should be understood by one skilled in the art that needing to obtain corresponding electrochemistry to measure amount of analyte
Signal；More specifically, blood of the beta-hydroxybutyric acid electrochemical sensor 5 of chronoamperometry by the unknown beta-hydroxybutyric acid concentration of acquisition
Then the corresponding current signal of sample is converted into the beta-hydroxybutyric acid concentration value of blood sample by Calibration equation.How can be with
Establish and the use of this Calibration equation is well known for those of ordinary skills.
The linear assessment of accuracy is divided into control group and test group.Using Abbott beta-hydroxybutyric acid test paper (Abbott,
FreeStyle Optium Blood β-Ketone Test Strips, Lot:It is used as reference product, 75001HO9) with this implementation
The beta-hydroxybutyric acid electrochemical sensor 5 of example is the linear ratio pair that test products carry out accuracy.Experimentation：By wound packages β-hydroxyl fourth
Sour electrochemical sensor 5 was placement under room temperature 30 minutes or more of 23 DEG C ± 2 DEG C.The requirement of blood sample is as follows：Red blood
Ball hematocrit ratio is adjusted to 42% ± 2%；Partial pressure of oxygen is controlled in 65mmHg ± 5mmHg；The beta-hydroxybutyric acid concentration of total 7 concentration sections
Whole blood sample：Concentration one is in 0-3mg/dL, concentration two is in 6 ± 3mg/dL, concentration three is in 20 ± 3mg/dL, concentration four
In 40 ± 3mg/dL, concentration five is in 60 ± 3mg/dL, and concentration six is in 80 ± 3mg/dL and concentration seven is in 100 ± 3mg/
dL.Each concentration section blood sample, reference product test is primary to obtain control group test value, test products retest No. 5 times meters
It calculates and obtains test cell mean, test result is shown in Table 2.It is vertical sit to test cell mean using control group test value as abscissa
It is denoted as linear comparison chart, as shown in Figure 2.
Table 2：The Accuracy evaluation test data of beta-hydroxybutyric acid electrochemical sensor 5
From table 2 it can be seen that the standard deviation (SD) and the coefficient of variation of the test value of beta-hydroxybutyric acid electrochemical sensor 5
(CV) smaller, repeatability is good.Figure it is seen that the test value of beta-hydroxybutyric acid electrochemical sensor 5 and the β-of Abbott
Related coefficient square (the R of hydroxybutyric acid test paper test value2) it is greater than 0.99, there is good accuracy.
Foundation Van't Hoff regular (Vant Hoff rule) and Arrhenius equation (Arrhenius equation),
7 days of accelerated ageing approximate can be considered as 14 days of accelerated ageing at 1 year, 65 DEG C under the conditions of true storage at room temperature at 65 DEG C
2 years for approximate being considered as under the conditions of true storage at room temperature, this will be understood by for those of ordinary skills.
For example, the reagent composition of patent CN100416266C medium-stable and its be used for electrochemical analyte method for measuring, be related to
Discuss that electrochemistry reagent composition can be such that medium-stable saves, using the experiment item for placing accelerated ageing in 2 weeks at 56 DEG C
Part.Be conducive to save the time cost of product development using the assessment mode of accelerated ageing.
The method that the present embodiment assesses a kind of thermal stability of the accelerated ageing of beta-hydroxybutyric acid electrochemical sensor 5 is：It is real
It tests and is divided into control group and test group, as a control group with the beta-hydroxybutyric acid electrochemical sensor that stores at 5 DEG C, at 65 DEG C of high temperature
Same a batch of beta-hydroxybutyric acid electrochemical sensor of storage tests blood sample respectively, obtains current value as test group
I5℃, I65℃；Calculate relative deviation, i.e. I65℃/I5℃* 100%-1.With relative deviation quantitative evaluation beta-hydroxybutyric acid electrochemical sensor
The thermal stability of 5 accelerated ageing.
The experiment condition of accelerated ageing：It will respectively be stored respectively with a batch of wound packages beta-hydroxybutyric acid electrochemical sensor 5
At 5 DEG C ± 1 DEG C, 3 days, 7 days and 10 days in 65 DEG C of ± 1 DEG C of baking ovens.
The experimentation of accelerated ageing assessment：Before experiment, by wound packages beta-hydroxybutyric acid electrochemical sensor 5 at 23 DEG C ± 2 DEG C
Placement under room temperature 30 minutes or more.Blood sample is as follows：Red blood cell hematocrit ratio is adjusted to 42% ± 2%；Oxygen is voltage-controlled
System is in 65mmHg ± 5mmHg；The whole blood sample of the beta-hydroxybutyric acid concentration of total 7 concentration sections：Concentration one is in 0-3mg/dL, dense
Degree two in 6 ± 3mg/dL, concentration three be in 20 ± 3mg/dL, concentration four is in 40 ± 3mg/dL, concentration five in 60 ±
3mg/dL, concentration six is in 80 ± 3mg/dL and concentration seven is in 100 ± 3mg/dL.Under the conditions of 5 DEG C ± 1 DEG C and 65 DEG C ± 1 DEG C
The beta-hydroxybutyric acid electrochemical sensor 5 of placement tests 7 concentration section blood samples, each concentration section blood sample, control respectively
5 calculating of product retest obtains control cell mean, and 5 calculating of test products retest obtains test cell mean.
The data processing of stability assessment experiment：Under same concentration section, the beta-hydroxybutyric acid electrochemical sensor for obtaining and storing at 5 DEG C is calculated
Control cell mean and the beta-hydroxybutyric acid electrochemical sensor stored at 65 DEG C test cell mean, calculate relative deviation I65℃/
I5℃* 100%-1.With the thermal stability of the accelerated ageing of relative deviation quantitative evaluation beta-hydroxybutyric acid electrochemical sensor.
Table 3：The assessment test data of 65 DEG C of beta-hydroxybutyric acid electrochemical sensor accelerated ageing 3 days
Table 4：The assessment test data of 65 DEG C of beta-hydroxybutyric acid electrochemical sensor accelerated ageing 7 days
Table 5：The assessment test data of 65 DEG C of beta-hydroxybutyric acid electrochemical sensor accelerated ageing 10 days
From table 3, table 4 and table 5 it can be seen that beta-hydroxybutyric acid electrochemical sensor stores 3 days, 7 days and 10 days items at 65 DEG C
Under part after accelerated ageing, the relative deviation of current value shows better heat stability (as shown in Figure 3), Ke Yiman within 15%
1 year validity period under the conditions of the true storage at room temperature of foot.
The foregoing is merely preference embodiments of the invention, are not intended to limit the scope of the present invention..Appoint
How within the spirit and principles in the present invention made any modifications, equivalent replacements, and improvements etc., should be included in of the invention
Within claims.
Claims (10)
1. a kind of beta-hydroxybutyric acid electrochemical sensor, which is characterized in that the sensor includes：
Substrate layer (1), there are sample introduction notch (11) for the end of substrate layer (1)；
Electrode layer (2) on the substrate layer (1), the electrode layer (2) include working electrode (21), to electrode (22),
Be switched on electrode (23) and conducting wire (24)；
Insulating layer (3) on the electrode layer (2) is provided with reagent window (31) at the insulating layer (3), the exposure work
Make electrode (21) and the working portion to electrode (22)；Region of the reagent window (31) to defined reaction reagent；
Middle interlayer (4) on the insulating layer (3), there are hydrophilic window (41) and exhaust windows for the middle interlayer (4)
(42), hydrophilic window (41) and exhaust window (42) and the reagent window (31) constitute sample introduction space.
2. a kind of beta-hydroxybutyric acid electrochemical sensor according to claim 1, which is characterized in that the sample introduction notch (11)
Shape be arc, sample introduction notch (11), which is arranged on substrate layer (1), corresponds to the working electrode (21) and to electrode (22)
One end.
3. a kind of beta-hydroxybutyric acid electrochemical sensor according to claim 1, which is characterized in that the hydrophilic window (41)
The accommodation space of blood sample sample introduction is constituted with exhaust window (42), reagent window (31) and sample introduction notch (11).
4. a kind of beta-hydroxybutyric acid electrochemical sensor according to claim 1, which is characterized in that on the middle interlayer (4)
With pressure sensitive adhesive, so that middle interlayer (4) is pasted on insulating layer (3)；The hydrophilic window (41) is by hydrophilic treated
PET, there is no pressure sensitive adhesives；The exhaust window (42) is opened wide setting and is penetrated through with outside air.
5. according to claim 1-4 it is one of any described in a kind of beta-hydroxybutyric acid electrochemical sensor, which is characterized in that the base
The material of lamella (1) is polyethylene terephthalate；The working electrode (21) and be carbon to the material of electrode (22)；It is described
It is switched on electrode (23) and the material of conducting wire (24) is silver；The material of the insulating layer (3) is hydrophobic material；The middle interlayer
It (4) is the one side glue of polyacrylic acid material.
6. according to claim 1-4 it is one of any described in a kind of beta-hydroxybutyric acid electrochemical sensor, which is characterized in that the examination
Reaction reagent defined by agent window includes:(a) enzyme；(b) enzymatic protective reagent；(c) electron mediator composition；(d) buffer solution；
(e) at least one surfactant；(f) at least one cellulose family high polymer.
7. a kind of beta-hydroxybutyric acid electrochemical sensor according to claim 6, which is characterized in that the enzyme includes β-hydroxyl fourth
Acidohydrogenase, diaphorase and coenzyme, the coenzyme are nicotinamide adenine dinucleotide.
8. a kind of beta-hydroxybutyric acid electrochemical sensor according to claim 6, which is characterized in that the enzymatic protective reagent is sugar
Class；The electron mediator composition includes hexaammine ruthenium chloride and azophenlyene, and azophenlyene is 5- toluphenazine Methylsulfate, 5- ethyl pheno
One of piperazine ethyl-sulfate, 1- methoxyl group -5- toluphenazine Methylsulfate are a variety of；The buffer solution is selected from biological buffer
Liquid or amphoteric ion buffer solution, buffer solution are molten using Good's buffer solution, organic acid buffer solution or phosphate-buffered
Liquid；The surfactant is nonionic surfactant；The cellulose family high polymer is hydroxymethyl cellulose.
9. a kind of beta-hydroxybutyric acid electrochemical sensor according to claim 8, which is characterized in that the enzymatic protective reagent is sea
Algae sugar；The electron mediator composition is hexaammine ruthenium chloride and 1- methoxyl group -5- toluphenazine Methylsulfate；The buffering is molten
Liquid is one of ACES, TES, MES or a variety of.
10. according to claim 1-4 it is one of any described in a kind of beta-hydroxybutyric acid electrochemical sensor, which is characterized in that it is described
Reaction reagent includes：The concentration of beta-hydroxybutyric dehydrogenase is 0.5-5wt.%, and the concentration of diaphorase is 0.5-5wt.%, coenzyme
Concentration be 0.5-5wt.%；Trehalose 0.1-1wt.%, the concentration of hexaammine ruthenium chloride are 0.5-5wt.%, 1- methoxyl group-
The concentration of 5- toluphenazine Methylsulfate is 0.005-0.05wt.%；The concentration of TES buffer solution is 10-50wt.%,Concentration be 0.3-3wt.%, the concentration of hydroxymethyl cellulose is 0.25-2.5wt.%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114965641A (en) * | 2022-05-23 | 2022-08-30 | 可孚医疗科技股份有限公司 | Beta-hydroxybutyric acid electrochemical test paper, preparation method and application |
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