CN213364568U - Living cell confocal imaging chamber - Google Patents
Living cell confocal imaging chamber Download PDFInfo
- Publication number
- CN213364568U CN213364568U CN202021821239.2U CN202021821239U CN213364568U CN 213364568 U CN213364568 U CN 213364568U CN 202021821239 U CN202021821239 U CN 202021821239U CN 213364568 U CN213364568 U CN 213364568U
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- Prior art keywords
- imaging
- hole
- chamber
- cavity
- perfusion
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- Expired - Fee Related
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- 238000010226 confocal imaging Methods 0.000 title claims abstract description 19
- 238000003384 imaging method Methods 0.000 claims abstract description 48
- 230000010412 perfusion Effects 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 230000000149 penetrating effect Effects 0.000 claims abstract description 4
- 239000012530 fluid Substances 0.000 claims description 12
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 5
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 31
- 230000015572 biosynthetic process Effects 0.000 description 6
- 239000006059 cover glass Substances 0.000 description 5
- 210000005056 cell body Anatomy 0.000 description 3
- 238000013459 approach Methods 0.000 description 2
- 238000007334 copolymerization reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000010859 live-cell imaging Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a living cell confocal imaging cell, which comprises a cell main body, an imaging hole cavity arranged at the top of the cell main body, a sample placing table arranged at the bottom of the imaging hole cavity, an observation hole arranged on the sample placing table in a penetrating way, and a perfusion liquid tube inlet hole and a perfusion liquid tube outlet hole which are respectively arranged at the top of the cell main body; the perfusion liquid tube inlet hole and the perfusion liquid tube outlet hole are also respectively communicated with the side wall of the imaging hole cavity body. Compared with the prior art, the utility model has the advantages of simple structure, easy operation, lower cost, etc.
Description
Technical Field
The utility model belongs to the technical field of image device, a living cell confocal formation of image cell is related to.
Background
Live cell imaging is a common approach in life science research, and confocal imaging by placing live cells cultured on an ultrathin coverslip in an imaging chamber is the most common low-cost approach.
In the living cell confocal imaging, a circular cover glass cultured with cells needs to be placed on an imaging chamber, and the cells need to be perfused if necessary, so that the currently available imaging chambers are more, but the imaging chambers have the defects of high price, inconvenience in use and the like.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a living cell copolymerization burnt formation of image cell for solve the higher and inconvenient problem of operation of changing the sample in-process and removing and adjusting the perfusion device production repeatedly of present copolymerization burnt culture dish cost.
The purpose of the utility model can be realized through the following technical scheme:
a living cell confocal imaging chamber comprises a chamber main body, an imaging hole cavity, an observation hole and perfusion liquid tube inlet holes, wherein the imaging hole cavity is formed in the top of the chamber main body;
the size of the observation hole is smaller than that of the bottom of the imaging cavity body, so that an annular sample placing table is formed between the observation hole and the side wall of the imaging cavity body;
the perfusion liquid tube inlet hole and the perfusion liquid tube outlet hole are also respectively communicated with the side wall of the imaging hole cavity body.
Furthermore, the cell main body is cylindrical, and the diameter of the top of the cell main body is 5 cm.
Furthermore, the material of the small chamber main body is polymethyl methacrylate.
Further, the imaging hole cavity is a cylindrical hole with the diameter of 18.5-25.5 mm.
Furthermore, the edge of the sample placing table is 1-1.5 mm.
Further, the thickness of the sample placing table is 1 mm.
Furthermore, the diameter of the observation hole is 16.5-22.5 mm.
Further, the observation hole and the imaging cavity body are coaxially arranged.
Furthermore, the perfusion tube inlet hole and the perfusion tube outlet hole are symmetrically arranged on two sides of the imaging hole cavity body.
Furthermore, the diameters of the perfusion tube inlet hole and the perfusion tube outlet hole are both 1-2 mm.
During operation, the cover glass cultured with living cells is arranged on the sample placing table by using vacuum glue, the perfusion liquid inlet pipe and the perfusion liquid outlet pipe are respectively connected with the living cells through the perfusion liquid inlet pipe and the perfusion liquid outlet pipe, then a living cell confocal imaging experiment can be carried out through the observation holes, and drug perfusion treatment can be carried out on the cells while observation is carried out according to needs.
Compared with the prior art, the utility model discloses because of using integrated living cell perfusion formation of image cell, the perfusion liquid pipe is directly inserted and is connected in the outer both sides of formation of image vestibule of formation of image room, and does not need extra device frame in formation of image vestibule top, the removal repeatedly and the adjustment problem of perfusion device when can avoiding placing the cell cover glass. And simultaneously, the utility model discloses an integration cell perfusion imaging cell that lives can punch according to the cover glass aperture of demand, has advantages such as the device structure is adjustable, the low price, and the price is only about fifteen to twenty-fifth of the cell imaging cell of foreign company living, shifts to this imaging cell after directly utilizing circular cover glass to cultivate the cell and carry out the cell imaging observation that lives, and the cost is only one third to one fifth that uses the confocal culture dish.
Drawings
FIG. 1 and FIG. 2 are schematic structural views of a confocal living cell imaging chamber according to example 1;
the notation in the figure is:
1-small chamber main body, 2-imaging hole cavity, 3-perfusion tube inlet hole, 4-perfusion tube outlet hole, 5-sample placing table and 6-observation hole.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
A living cell confocal imaging chamber comprises a cylindrical chamber main body 1 with the diameter of 5cm, a cylindrical imaging hole cavity 2 which is arranged at the top of the chamber main body 1 and has the diameter of 18.5-25.5mm, an observation hole 6 which is arranged at the bottom of the imaging hole cavity 2 in a penetrating way and has the diameter of 16.5-22.5mm, and perfusion fluid tube inlet holes 3 and perfusion fluid tube outlet holes 4 which are symmetrically arranged at two sides of the cylindrical imaging hole cavity 2 and have the diameter of 1-2 mm; a sample placing table 5 with the edge width of 1-1.5mm and the thickness of 1mm is formed between the observation hole 6 and the side wall of the imaging hole cavity 2, and two ends of the perfusion fluid tube inlet hole 3 and the perfusion fluid tube outlet hole 4 are respectively communicated with the top of the small chamber main body 1 and the side wall of the imaging hole cavity 2. Further, the cell body 1 is made of polymethyl methacrylate, and the observation hole 6 is provided coaxially with the imaging hole cavity 2.
The following examples are carried out on the premise of the technical solution of the present invention, and detailed embodiments and specific operation procedures are given, but the scope of the present invention is not limited to the following examples.
Example 1:
as shown in fig. 1 and fig. 2, a living cell confocal imaging chamber includes a cylindrical chamber body 1 with a diameter of 5cm, a cylindrical imaging hole cavity 2 with a diameter of 18.5mm and provided at the top of the chamber body 1, an observation hole 6 with a diameter of 16.5mm and provided at the bottom of the imaging hole cavity 2, and a perfusion tube inlet hole 3 and a perfusion tube outlet hole 4 with a diameter of 1mm and symmetrically provided at two sides of the cylindrical imaging hole cavity 2; a sample placing table 5 with the edge width of 1mm and the thickness of 1mm is formed between the observation hole 6 and the side wall of the imaging hole cavity 2, and the two ends of the perfusion fluid tube inlet hole 3 and the perfusion fluid tube outlet hole 4 are respectively communicated with the top of the small chamber main body 1 and the side wall of the imaging hole cavity 2. Further, the cell body 1 is made of polymethyl methacrylate, and the observation hole 6 is provided coaxially with the imaging hole cavity 2.
Example 2:
a living cell confocal imaging chamber comprises a cylindrical chamber main body 1 with the diameter of 5cm, a cylindrical imaging hole cavity 2 which is arranged at the top of the chamber main body 1 and has the diameter of 25.5mm, an observation hole 6 which is arranged at the bottom of the imaging hole cavity 2 in a penetrating way and has the diameter of 22.5mm, and perfusion fluid tube inlet holes 3 and perfusion fluid tube outlet holes 4 which are symmetrically arranged at two sides of the cylindrical imaging hole cavity 2 and have the diameter of 2 mm; a sample placing table 5 with the edge width of 1.5mm and the thickness of 1mm is formed between the observation hole 6 and the side wall of the imaging hole cavity 2, and the two ends of the perfusion fluid tube inlet hole 3 and the perfusion fluid tube outlet hole 4 are respectively communicated with the top of the cell main body 1 and the side wall of the imaging hole cavity 2. Further, the cell body 1 is made of polymethyl methacrylate, and the observation hole 6 is provided coaxially with the imaging hole cavity 2.
The embodiments described above are intended to facilitate the understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention according to the disclosure of the present invention.
Claims (10)
1. A living cell confocal imaging chamber is characterized by comprising a chamber main body (1), an imaging hole cavity (2) arranged at the top of the chamber main body (1), an observation hole (6) penetrating the bottom of the imaging hole cavity (2), and a perfusion fluid tube inlet hole (3) and a perfusion fluid tube outlet hole (4) which are respectively arranged at the top of the chamber main body (1);
the size of the observation hole (6) is smaller than the size of the bottom of the imaging hole cavity (2), so that an annular sample placing table (5) is formed between the observation hole (6) and the side wall of the imaging hole cavity (2);
the perfusion liquid tube inlet hole (3) and the perfusion liquid tube outlet hole (4) are also respectively communicated with the side wall of the imaging hole cavity (2).
2. A confocal imaging chamber in living cells according to claim 1, characterized in that said chamber body (1) is cylindrical with a diameter of 5cm at the top.
3. A confocal imaging chamber in living cells according to claim 1, characterized in that the material of said chamber body (1) is polymethyl methacrylate.
4. A confocal imaging chamber in living cells according to claim 1, characterized in that said imaging bore cavity (2) is a cylindrical bore with a diameter of 18.5-25.5 mm.
5. A confocal imaging chamber of living cells according to claim 1, characterized in that said sample-holding stage (5) has a lateral width of 1-1.5 mm.
6. A confocal imaging chamber in living cells according to claim 5, characterized in that said sample-holding stage (5) has a thickness of 1 mm.
7. A confocal imaging chamber in living cells according to claim 1, characterized in that said observation hole (6) has a diameter comprised between 16.5 and 22.5 mm.
8. A confocal imaging chamber in living cells according to claim 1, characterized in that said viewing port (6) is arranged coaxially to the imaging port cavity (2).
9. The confocal living cell imaging chamber as claimed in claim 1, wherein the perfusion tube inlet (3) and the perfusion tube outlet (4) are symmetrically disposed on both sides of the imaging hole cavity (2).
10. A confocal imaging chamber in living cells according to claim 1, wherein the diameters of the perfusion tube inlet (3) and perfusion tube outlet (4) are both 1-2 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021821239.2U CN213364568U (en) | 2020-08-27 | 2020-08-27 | Living cell confocal imaging chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021821239.2U CN213364568U (en) | 2020-08-27 | 2020-08-27 | Living cell confocal imaging chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213364568U true CN213364568U (en) | 2021-06-04 |
Family
ID=76147746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021821239.2U Expired - Fee Related CN213364568U (en) | 2020-08-27 | 2020-08-27 | Living cell confocal imaging chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213364568U (en) |
-
2020
- 2020-08-27 CN CN202021821239.2U patent/CN213364568U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210604 |