CN215250926U - HAT selective medium for monoclonal antibody preparation - Google Patents
HAT selective medium for monoclonal antibody preparation Download PDFInfo
- Publication number
- CN215250926U CN215250926U CN202121132155.2U CN202121132155U CN215250926U CN 215250926 U CN215250926 U CN 215250926U CN 202121132155 U CN202121132155 U CN 202121132155U CN 215250926 U CN215250926 U CN 215250926U
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- culture
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- inner chamber
- monoclonal antibody
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- 239000006152 selective media Substances 0.000 title claims abstract description 24
- 238000003780 insertion Methods 0.000 claims abstract description 17
- 230000037431 insertion Effects 0.000 claims abstract description 17
- 238000000926 separation method Methods 0.000 claims abstract description 15
- 230000016784 immunoglobulin production Effects 0.000 claims description 12
- 238000005192 partition Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 abstract description 12
- 238000002474 experimental method Methods 0.000 abstract description 9
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical class O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002719 pyrimidine nucleotide Substances 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The utility model discloses a monoclonal antibody preparation HAT selective medium, include: the culture device comprises a base body seat plate and a culture inner chamber arranged at the upper end of the base body seat plate, wherein the culture inner chamber and the base body seat plate are integrally formed; the crossed separation frame is arranged inside the culture inner chamber, a crossed insertion plate is arranged on the outer wall of the crossed separation frame, and the crossed separation frame is inserted and installed with a crossed insertion slot inside the culture inner chamber through the crossed insertion plate; the hood frame, it is installed the upper end of base member bedplate, the lower extreme of hood frame is provided with four grafting connecting rods, and grafting connecting rod and hood frame integrated into one piece set up, and it has realized once cultivateing the function of a plurality of biological antibodies in the inside of single selective culture medium to distinguish the preparation experiment of contrast to a plurality of biological antibodies under the prerequisite of same environment, avoided causing the waste of experimental device, be difficult to keep the same problem of a plurality of culture medium environmental conditions.
Description
Technical Field
The utility model relates to a selective culture medium technical field especially relates to a HAT selective culture medium for monoclonal antibody preparation.
Background
One of the indispensable devices in the experiment, selective medium, is designed according to the special nutritional requirements of a certain microorganism or resistance to certain special chemical and physical factors, and is a medium capable of selectively distinguishing the microorganism, HAT selective medium is designed according to the biosynthesis pathway of hypoxanthine nucleotide and pyrimidine nucleotide, and HAT medium is a cell culture medium containing the three substances.
The prior HAT selective culture medium can not distinguish and compare a plurality of biological antibodies under the premise of the same environment in the using process, and the distinguishing and comparing need to use a plurality of culture media, so that the waste of experimental equipment is caused, and the environmental conditions of the plurality of culture media are difficult to keep the same, therefore, the HAT selective culture medium for monoclonal antibody preparation is provided.
SUMMERY OF THE UTILITY MODEL
It is an object of the present invention to solve at least the above problems and to provide at least the advantages which will be described later.
The utility model discloses it is still another purpose to provide a monoclonal antibody preparation HAT selective medium, has realized once cultivateing the function of a plurality of biological antibodies in the inside of single selective medium to distinguish the preparation experiment of contrast to a plurality of biological antibodies under the prerequisite of same environment, avoided causing the waste of experimental device, be difficult to keep the same problem of a plurality of culture medium environmental conditions.
In order to achieve the above and other objects, the present invention adopts the following technical solutions:
a HAT selective medium for monoclonal antibody production, comprising: the culture device comprises a base body seat plate and a culture inner chamber arranged at the upper end of the base body seat plate, wherein the culture inner chamber and the base body seat plate are integrally formed;
the crossed separation frame is arranged inside the culture inner chamber, a crossed insertion plate is arranged on the outer wall of the crossed separation frame, and the crossed separation frame is inserted and installed with a crossed insertion slot inside the culture inner chamber through the crossed insertion plate;
the top cover frame is arranged at the upper end of the base seat plate, the lower end of the top cover frame is provided with four plug-in connecting rods, and the plug-in connecting rods and the top cover frame are integrally formed;
the central port is arranged at the central position of the upper end of the top cover frame, a perspective mirror is arranged inside the central port, and the perspective mirror is in mechanical sealing connection with the central port;
the top cover frame is inserted into the connecting rod insertion holes in the base body base plate through four insertion connecting rods;
and the end face pull grooves are arranged on two sides of the central port and are integrally formed with the top cover frame.
Preferably, four the inside of grafting connecting rod all is provided with the screw hole, and screw hole and grafting connecting rod integrated into one piece set up, the inside of connecting rod jack is provided with the screw channel and connects the thread groove, and screw channel and connection thread groove and base member bedplate integrated into one piece set up.
Preferably, the threaded hole channel and the connecting screw groove are located on the same horizontal line, threaded bolts are installed inside the threaded hole, the threaded hole channel and the connecting screw groove, and the inserting connecting rod is fixedly connected with the base seat plate through the threaded bolts.
Preferably, the end face pull groove is internally provided with a lifting rod, the length of the lifting rod is equal to the diameter of the end face pull groove, and the lifting rod and the top cover frame are integrally formed.
Preferably, the inside central position of the perspective mirror is provided with a vent hole, and the vent hole and the perspective mirror are integrally formed.
Preferably, four linear frames are arranged on the inner wall of the culture inner chamber, the linear frames and the culture inner chamber are integrally formed, and four ends of the cross-shaped separation frame are in contact connection with the culture inner chamber through the linear frames.
The utility model discloses at least, include following beneficial effect:
1. the utility model discloses HAT selective medium once can cultivate four kinds of different biological antibodies simultaneously, the cell of these four kinds of biological antibodies is cultivateed in the inside of same culture medium, the preparation experiment of contrast can be distinguished to the result of cultivateing the experiment, the function of once cultivateing a plurality of biological antibodies in the inside of single selective medium has been realized, thereby the preparation experiment of contrast is distinguished to a plurality of biological antibodies under the prerequisite of same environment, the waste that causes experimental apparatus has been avoided, be difficult to keep the same problem of a plurality of culture medium environmental conditions.
2. The air holes formed in the central position in the perspective mirror can facilitate air inflow of the HAT selective culture medium, so that biological antibodies can be cultured in an auxiliary manner, and the four linear frames arranged on the inner wall of the culture inner chamber can facilitate the close contact and connection of the cross separation frame culture inner chamber, thereby avoiding the problem of liquid stringing of different biological antibodies.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a HAT selective medium for monoclonal antibody production according to the present invention;
FIG. 2 is a schematic diagram showing the structure of a hood framework of a HAT selective medium for monoclonal antibody production according to the present invention;
FIG. 3 is a schematic view of the internal structure of a culture chamber of the HAT selective medium for monoclonal antibody production according to the present invention;
FIG. 4 is a schematic diagram showing the structure of a cross partition frame of the HAT selective medium for monoclonal antibody production according to the present invention.
Detailed Description
The present invention is described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description.
As shown in fig. 1 to 4, a HAT selective medium for monoclonal antibody production, comprising: the culture device comprises a base seat plate 1 and a culture inner chamber 11 arranged at the upper end of the base seat plate 1, wherein the culture inner chamber 11 and the base seat plate 1 are integrally formed; the crossed separation frame 12 is arranged inside the culture inner chamber 11, a crossed plug board 17 is arranged on the outer wall of the crossed separation frame 12, and the crossed separation frame 12 is inserted and installed with a crossed slot inside the culture inner chamber 11 through the crossed plug board 17; the top cover frame 2 is arranged at the upper end of the base seat plate 1, the lower end of the top cover frame 2 is provided with four plug-in connecting rods 8, and the plug-in connecting rods 8 and the top cover frame 2 are integrally formed; the central port 5 is arranged at the central position of the upper end of the top cover frame 2, a perspective mirror 6 is arranged inside the central port 5, and the perspective mirror 6 is in mechanical sealing connection with the central port 5; the connecting rod insertion holes 14 are formed in the base body seat plate 1, and the top cover frame 2 is inserted into the connecting rod insertion holes 14 in the base body seat plate 1 through the four insertion connecting rods 8; and the end face pull grooves 3 are arranged on two sides of the central port 5, and the end face pull grooves 3 and the top cover frame 2 are integrally formed.
In the above scheme, the HAT selective medium can simultaneously culture four different biological antibodies once, the cells of the four biological antibodies are cultured in the same medium, the preparation experiment for distinguishing and comparing the results of the culture experiment can be carried out, the function of once culturing a plurality of biological antibodies in the single selective medium is realized, thereby distinguishing and comparing the preparation experiment for a plurality of biological antibodies on the premise of the same environment, the waste of experimental equipment is avoided, and the problem that the environmental conditions of a plurality of media are the same is difficult to keep.
In a preferred scheme, four threaded holes 9 are formed in the inserting connecting rods 8, the threaded holes 9 and the inserting connecting rods 8 are integrally formed, threaded hole channels 15 and connecting screw grooves 16 are formed in the connecting rod inserting holes 14, and the threaded hole channels 15 and the connecting screw grooves 16 are integrally formed with the base seat plate 1.
In the above scheme, four the screw holes that the inside of grafting connecting rod all set up play the effect that the grafting connecting rod of being convenient for corresponds with screw hole and connection thread groove and be connected, and the screw hole that the inside of connecting rod jack set up plays the effect that the screw thread bolt of being convenient for was installed with connection thread groove.
In a preferable scheme, the threaded hole 9, the threaded hole passage 15 and the connecting screw groove 16 are located on the same horizontal line, the threaded bolt 7 is installed inside the threaded hole 9, the threaded hole passage 15 and the connecting screw groove 16, and the plug-in connecting rod 8 is fixedly connected with the base seat plate 1 through the threaded bolt 7.
In the scheme, the threaded bolt installed inside the threaded hole, the threaded hole channel and the connecting thread groove is connected and fixed with the plug-in connecting rod and the base seat plate, so that the top cover frame is fixedly installed at the upper end of the base seat plate.
In a preferable scheme, a lifting rod 4 is arranged inside the end face pull groove 3, the length of the lifting rod 4 is equal to the diameter of the end face pull groove 3, and the lifting rod 4 and the top cover frame 2 are integrally formed.
In the above scheme, the lifting rod arranged in the end face pull groove plays a role in facilitating lifting of the top cover frame, and the length of the lifting rod is equal to the diameter of the end face pull groove, so that the lifting rod can be arranged in the end face pull groove.
In a preferable scheme, an air hole 10 is formed in the center of the inside of the see-through mirror 6, and the air hole 10 and the see-through mirror 6 are integrally formed.
In the scheme, the air holes arranged at the inner central position of the photoscope have the function of facilitating air inflow of the HAT selective medium, so that the culture of the biological antibody is assisted.
In a preferred embodiment, four linear frames 13 are disposed on the inner wall of the cultivation inner chamber 11, the linear frames 13 are integrally formed with the cultivation inner chamber 11, and the four ends of the cross-shaped partition frame 12 are in contact connection with the cultivation inner chamber 11 through the linear frames 13.
In the scheme, the four linear frames arranged on the inner wall of the culture inner chamber play a role in facilitating the close contact and connection of the culture inner chamber of the cross separation frame, so that the problem of liquid stringing of different biological antibodies is avoided.
While the embodiments of the invention have been described above, it is not intended to be limited to the details shown, or described, but rather to cover all modifications, which would come within the scope of the invention, as defined by the claims and the equivalents thereof, and which fall within the limits of the claims and the equivalents thereof.
Claims (6)
1. A HAT selective medium for monoclonal antibody production, comprising:
the culture device comprises a base body seat plate and a culture inner chamber arranged at the upper end of the base body seat plate, wherein the culture inner chamber and the base body seat plate are integrally formed;
the crossed separation frame is arranged inside the culture inner chamber, a crossed insertion plate is arranged on the outer wall of the crossed separation frame, and the crossed separation frame is inserted and installed with a crossed insertion slot inside the culture inner chamber through the crossed insertion plate;
the top cover frame is arranged at the upper end of the base seat plate, the lower end of the top cover frame is provided with four plug-in connecting rods, and the plug-in connecting rods and the top cover frame are integrally formed;
the central port is arranged at the central position of the upper end of the top cover frame, a perspective mirror is arranged inside the central port, and the perspective mirror is in mechanical sealing connection with the central port;
the top cover frame is inserted into the connecting rod insertion holes in the base body base plate through four insertion connecting rods;
and the end face pull grooves are arranged on two sides of the central port and are integrally formed with the top cover frame.
2. The HAT selective medium for monoclonal antibody production according to claim 1, wherein each of the four plug-in connection rods has a threaded hole formed therein and integrally formed therewith, and the rod insertion hole has a threaded hole and a connection screw groove formed therein and integrally formed therewith and a base plate.
3. The HAT selective medium for monoclonal antibody production according to claim 2, wherein the threaded hole, the threaded hole and the connecting screw groove are located on the same horizontal line, threaded pins are installed inside the threaded hole, the threaded hole and the connecting screw groove, and the plug-in connection rod is fixedly connected to the base plate through the threaded pins.
4. The HAT selective medium for monoclonal antibody production according to claim 1, wherein a lift rod is provided inside the end face groove, the length of the lift rod is equal to the diameter of the end face groove, and the lift rod is provided integrally with the top cover frame.
5. The HAT selective medium for monoclonal antibody production according to claim 1, wherein a vent hole is formed in the central position of the inside of the see-through lens, and the vent hole is formed integrally with the see-through lens.
6. The HAT selective medium for monoclonal antibody production according to claim 1, wherein four linear frames are provided on the inner wall of the culture chamber, and the linear frames are integrally formed with the culture chamber, and the four ends of the cross-shaped partition frame are in contact connection with the culture chamber through the linear frames.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121132155.2U CN215250926U (en) | 2021-05-25 | 2021-05-25 | HAT selective medium for monoclonal antibody preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121132155.2U CN215250926U (en) | 2021-05-25 | 2021-05-25 | HAT selective medium for monoclonal antibody preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215250926U true CN215250926U (en) | 2021-12-21 |
Family
ID=79458874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121132155.2U Expired - Fee Related CN215250926U (en) | 2021-05-25 | 2021-05-25 | HAT selective medium for monoclonal antibody preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215250926U (en) |
-
2021
- 2021-05-25 CN CN202121132155.2U patent/CN215250926U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211221 |