CN212077054U - Enzyme method large-scale production system for Rh2 - Google Patents
Enzyme method large-scale production system for Rh2 Download PDFInfo
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- CN212077054U CN212077054U CN201990000212.6U CN201990000212U CN212077054U CN 212077054 U CN212077054 U CN 212077054U CN 201990000212 U CN201990000212 U CN 201990000212U CN 212077054 U CN212077054 U CN 212077054U
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Abstract
Rh2 enzyme process scale production system relates to biological catalysis technology technical field, this production system includes reation kettle, cooling water circulating device, hot steam circulating device, the acid storage tank, the alkali storage tank, a detection device for detecting the reaction degree in reation kettle, a solid-liquid separation equipment for carrying out solid-liquid separation to the material that comes out from reation kettle and handle, a washing tank for carrying out abluent washing to the material after a solid-liquid separation, a secondary solid-liquid separation equipment for carrying out solid-liquid separation to the material that comes out from washing tank and handle, and a drying device for carrying out drying process to the material after secondary solid-liquid separation. The production system has the advantages of simple structure, complete functions, compact arrangement, space and cost saving, and can realize automatic control on temperature and pH value and save manpower.
Description
Technical Field
The invention relates to the technical field of biological catalysis processes, in particular to an industrial production system for large-scale production of Rh2 by using a biological enzyme catalysis technology.
Background
Rh2 is one of the main components of ginsenoside with pharmacological activity in ginseng, is a monomer component with relatively remarkable anti-cancer activity in ginsenoside, almost represents all positive effects in ginseng, and is considered as the most potential natural anti-cancer substance at present.
Because the content of Rh2 in ginseng is very rare and extraction is very difficult, companies represented by the Bontai organism develop a method for producing Rh2 by using a biological enzyme catalysis technology, and the method has the advantages of simple operation, short time, energy conservation and environmental protection. Although a lot of processes for producing Rh2 by using a bio-enzyme catalysis technology are reported at present, no report is found about an industrial production system of Rh2 enzyme method. It is known in the art that when a new process is applied to large-scale industrial production from pilot plant and pilot plant, there are some technical difficulties, and these technical difficulties often become road barrows preventing the enzymatic preparation process of Rh2 from being applied to large-scale industrial production.
Disclosure of Invention
In view of the above-mentioned disadvantages of the prior art, the present invention aims to provide a system suitable for Rh2 enzyme-catalyzed mass production, so that the Rh2 enzyme-catalyzed production process developed at present can be more conveniently, more quickly and directly applied to mass industrial production, thereby filling the gap of Rh2 enzyme-catalyzed industrial production system.
In order to achieve the purpose, the invention provides an Rh2 enzyme-process large-scale production system, which comprises a reaction kettle, a cooling water circulating device, a hot steam circulating device, an acid storage tank, an alkali storage tank, a detection device for detecting the reaction degree in the reaction kettle, a primary solid-liquid separation device for performing solid-liquid separation treatment on materials discharged from the reaction kettle, a washing tank for cleaning the materials subjected to the primary solid-liquid separation, a secondary solid-liquid separation device for performing solid-liquid separation treatment on the materials discharged from the washing tank, and a drying device for drying the materials subjected to the secondary solid-liquid separation; wherein:
the reaction kettle is provided with a feeding port, a discharging port, a thermometer for measuring the temperature in the kettle, a pH meter for measuring the pH value in the kettle and a stirring device for stirring the materials in the kettle, and the outer wall of the reaction kettle is provided with a jacket layer for circulating cooling water and hot steam;
the cooling water circulating device is communicated with the jacket layer of the reaction kettle through a pipeline and is used for providing cooling water for reducing the temperature in the kettle for the jacket layer of the reaction kettle;
the hot steam circulating device is communicated with the jacket layer of the reaction kettle through a pipeline and is used for providing hot steam for raising the temperature in the kettle for the jacket layer of the reaction kettle;
the acid storage tank is communicated with the interior of the reaction kettle through a pipeline;
the alkali storage tank is communicated with the interior of the reaction kettle through a pipeline;
the primary solid-liquid separation device is arranged below the discharge hole of the reaction kettle;
the washing tank comprises a tank body and a stirring device for stirring the materials in the tank body;
the secondary solid-liquid separation device is arranged below a discharge hole of the washing tank.
The inner walls of the reaction kettle, the water washing tank, the acid storage tank and the alkali storage tank in the production system are all provided with corrosion-resistant layers resistant to acid and alkali, or the corresponding kettle body and the corresponding tank body are made of materials resistant to acid and alkali.
The detection device in the above production system may be any device known at present that can detect the degree of progress of the reaction in the reaction vessel, such as a liquid chromatograph or the like.
The solid-liquid separation device in the production system can be any device which can separate the solid and the liquid in the material and can not damage the components in the material, such as a centrifuge, a plate-and-frame filter press, a membrane concentration device and the like.
The drying device in the production system described above may be any device known at present that is capable of removing moisture from the Rh2 product without reducing the Rh2 content and without disrupting the Rh2 structure, such as a vacuum dryer, a freeze dryer, or the like.
Preferably, in the production system provided by the invention, the acid storage tank and the alkali storage tank are arranged above the reaction kettle, so that the acid liquid and the alkali liquid can naturally flow into the reaction kettle under the action of gravity, and the use of a pump is reduced.
Preferably, the production system provided by the invention further comprises a condensing device for cooling the solvent heated and volatilized in the kettle of the reaction kettle, wherein the condensing device is arranged above the reaction kettle and communicated with the reaction kettle through a pipeline to form a channel for the cooled solvent to flow back to the kettle.
Preferably, the production system provided by the invention further comprises a transfer device for transferring the materials, and the materials can be conveniently put into the reaction kettle through the transfer device, and the materials after the primary solid-liquid separation are transferred into the water washing tank, or the materials after the secondary solid-liquid separation are transferred into the drying device. The transfer device may be a plurality of fixed devices respectively arranged beside each device needing to transfer the materials, or may be one or more non-fixed devices which can freely move among the devices needing to transfer the materials. From the viewpoint of resource and space saving, the transfer device is preferably a non-fixed device, and preferably, the transfer device is a traveling crane.
In order to realize automation of the system, reduce manual operation, and reduce labor cost, preferably, the production system provided by the present invention further comprises an automatic control system, which comprises a temperature automatic control system and a pH automatic control system, wherein:
the temperature automatic control system comprises a temperature reading device, a temperature control unit, a cooling water electromagnetic valve and a hot steam electromagnetic valve; one end of the temperature reading device is electrically connected with the thermometer, the other end of the temperature reading device is electrically connected with the temperature control unit, the cooling water electromagnetic valve is arranged on the cooling water circulating device and used for controlling the cooling water circulating device to be opened and closed, the hot steam electromagnetic valve is arranged on the hot steam circulating device and used for controlling the hot steam circulating device to be opened and closed, and the temperature control unit respectively controls the cooling water electromagnetic valve and the hot steam electromagnetic valve to be opened and closed;
the pH automatic control system comprises a pH reading device, a pH control unit, an acid liquor electromagnetic valve and an alkali liquor electromagnetic valve; the pH control unit is used for controlling the opening and closing of the acid liquor electromagnetic valve and the alkali liquor electromagnetic valve respectively.
In order to better prevent environmental pollution and realize sustainable development, preferably, the production system provided by the invention further comprises a sewage treatment system for performing purification treatment on the liquid after solid-liquid separation treatment.
Has the advantages that:
the Rh2 enzyme method scale production system provided by the invention has the advantages of simple structure, complete functions, compact arrangement, space and cost saving, and can realize automatic control on temperature and pH value and save manpower. In addition, a water washing kettle and a solid-liquid separation device corresponding to the water washing kettle are independently arranged, so that continuous and uninterrupted reaction of two batches of materials can be realized.
Drawings
Fig. 1 is a schematic structural diagram of an enzymatic large-scale production system of Rh2 provided in the embodiment of the present invention;
in the figure:
1. the system comprises a reaction kettle, 11, a feed inlet, 12, a discharge outlet, 13, a sampling port, 14, a jacket layer, 141, a hot steam inlet, 142, a cooling water inlet, 143, a hot steam outlet, 144, a cooling water outlet, 15, a stirrer, 16, a thermometer, 17, a pH meter, 2, a water washing tank, 21, a discharge outlet, 22, a stirrer, 3, an acid storage tank, 31, an acid liquor electromagnetic valve, 4, an alkali storage tank, 41, an alkali liquor electromagnetic valve, 5, a condenser, 51, a condensed water inlet, 52, a condensed water outlet, 6, a hot steam circulating device, 61, a hot steam electromagnetic valve, 7, a cooling water circulating device, 71, a cooling water electromagnetic valve, 8, a centrifuge and 9, a sewage treatment device.
Detailed Description
The present invention will be described in further detail with reference to the following drawings and specific examples, which are illustrative of the present invention and are not intended to limit the present invention.
As shown in fig. 1, the present embodiment provides a large-scale production system of Rh2 by an enzymatic method, the production system includes a reaction kettle 1, a water washing tank 2, an acid storage tank 3, an alkali storage tank 4, a condenser 5, a hot steam circulation device 6, a cooling water circulation device 7, a centrifuge 8, a sewage treatment device 9, a traveling crane (not shown), a liquid chromatograph (not shown), and a vacuum dryer (not shown), and the inner walls of the reaction kettle 1, the water washing tank 2, the acid storage tank 3, and the alkali storage tank 4 are all provided with corrosion resistant layers resistant to acid and alkali. Wherein:
the acid storage tank 3 and the alkali storage tank 4 are arranged above the reaction kettle 1 and are respectively communicated with the interior of the reaction kettle 1 through pipelines, and an acid liquor electromagnetic valve 31 and an alkali liquor electromagnetic valve 41 are respectively arranged at one end of each pipeline close to the reaction kettle 1;
the top of the reaction kettle 1 is provided with a feeding port 11, the bottom of the reaction kettle is provided with a discharge port 12 and a sampling port 13, the outer wall of the reaction kettle is provided with a jacket layer 14, the upper part of the jacket layer 14 is respectively provided with a hot steam inlet 141 and a cooling water outlet 144, the lower part of the jacket layer 14 is respectively provided with a cooling water inlet 142 and a hot steam outlet 143, the hot steam inlet 141 and the hot steam outlet 143 are respectively arranged at two sides of the reaction kettle, and the cooling water inlet 142 and the cooling water outlet 144 are respectively arranged at two sides of the reaction kettle; the reaction kettle 1 is also provided with a stirrer 15, a thermometer 16 and a pH meter 17 which extend into the kettle, and a condenser 5 which is communicated with the interior of the reaction kettle 1 through a pipeline;
the hot steam circulating device 6 is respectively communicated with a hot steam inlet 141 and a hot steam outlet 143 through pipelines to form circulating circulation of hot steam in the jacket layer 14, and the hot steam circulating device 6 is opened and closed through a hot steam electromagnetic valve 61;
the cooling water circulating device 7 is respectively communicated with a cooling water inlet 142 and a cooling water outlet 144 and a condensed water inlet 51 and a condensed water outlet 52 on the condenser 5 through pipelines to form circulating circulation of cooling water in the jacket layer 14 and circulating circulation in the condenser 5, and the cooling water circulating device 7 conveys and stops the cooling water circulation in the jacket layer 14 through a cooling water electromagnetic valve 71 independently arranged on the cooling water circulating pipeline;
the centrifugal machine 8 in the embodiment is composed of a primary centrifugal machine and a secondary centrifugal machine, wherein the primary centrifugal machine and the secondary centrifugal machine are respectively arranged below the reaction kettle 1 and the washing tank 2, a feed inlet of the primary centrifugal machine is communicated with a discharge outlet 12 of the reaction kettle 1 through a pipeline, a feed inlet of the secondary centrifugal machine is communicated with a discharge outlet 21 of the washing tank 2 through a pipeline, and a liquid outlet of the centrifugal machine 8 is communicated with the sewage treatment device 9 through a pipeline;
in addition, the enzymatic large-scale production system of Rh2 provided in this embodiment further includes a temperature automatic control system and a pH automatic control system, wherein the temperature automatic control system is composed of a temperature reading device (not shown), a temperature control unit (not shown), a hot steam electromagnetic valve 61 and a cooling water electromagnetic valve 71; the pH automatic control system is composed of a pH reading device (not shown), a pH control unit (not shown), an acid liquor electromagnetic valve 31 and an alkali liquor electromagnetic valve 41.
When the production system provided by the embodiment is applied, required materials are firstly put into the reaction kettle 1 through the feeding port 11, the required temperature is set for the temperature control unit, the required pH value is set for the pH control unit, and the stirrer 15, the temperature reading device, the pH reading device and the cooling water circulating device are started;
the thermometer 16 measures the temperature in the reaction kettle 1 in real time, the temperature reading device reads an actual temperature value measured by the thermometer 16 and transmits the actual temperature value to the temperature control unit, the temperature control unit compares the actual temperature value with a set temperature value, if the actual temperature value is lower than the set temperature value, the temperature control unit opens the hot steam circulating device 6 by opening the hot steam electromagnetic valve 61 to provide circulating hot steam for the jacket layer 14 so as to heat the reaction kettle 1; when the actual temperature value is equal to the set temperature value, the temperature control unit closes the hot steam circulating device 6 by closing the hot steam electromagnetic valve 61, so as to stop continuously heating the reaction kettle 1; if the actual temperature value is higher than the set temperature value, the temperature control unit opens the cooling water electromagnetic valve 71 to circularly convey cooling water to the jacket layer 14 so as to cool the reaction kettle 1; when the actual temperature value is equal to the set temperature value, the temperature control unit stops conveying the cooling water to the jacket layer 14 by closing the cooling water electromagnetic valve 71, so as to stop continuously cooling the reaction kettle 1.
Meanwhile, the pH meter 17 measures the pH value in the reaction kettle 1 in real time, the pH reading device reads the actual pH value measured by the pH meter 17 and transmits the actual pH value to the pH control unit, the pH control unit compares the actual pH value with the set pH value, and if the actual pH value is higher than the set pH value, the pH control unit transmits acid liquor into the reaction kettle 1 by opening the acid liquor electromagnetic valve 31; when the actual pH value is equal to the set pH value, the pH control unit stops conveying the acid liquor into the reaction kettle 1 by closing the acid liquor electromagnetic valve 31; if the actual pH value is lower than the set pH value, the pH control unit opens the alkali liquor electromagnetic valve 41 to convey alkali liquor into the reaction kettle 1; when the actual pH value is equal to the set pH value, the pH control unit stops conveying the alkali liquor into the reaction kettle 1 by closing the alkali liquor electromagnetic valve 41;
simultaneously, volatile solvent in reation kettle 1 is heated and volatilizes back and is gone into condenser 5 through the pipeline and in being cooled down, continue participating in the reaction in backward flow to reation kettle 1 through the pipeline, one can save the solvent cost, two can prevent to rise because of too much gas production pressure in reation kettle 1.
Meanwhile, sampling is carried out in real time through a sampling port 13, the degree of reaction proceeding in the reaction kettle is detected through a liquid chromatograph, after the reaction is completed, the materials are discharged into a centrifuge 8 through a discharge port 12 to be subjected to solid-liquid separation treatment, the separated liquid flows into a sewage treatment device 9 through a pipeline to be subjected to purification treatment, after the centrifugation is finished, the separated solid materials are transferred into a water washing tank 2 through a travelling crane, a stirrer 22 is started to be stirred after water is added, the liquid is discharged into the centrifuge 8 through a discharge port 21 to be subjected to solid-liquid separation treatment after being uniformly stirred, the separated liquid flows into the sewage treatment device 9 through a pipeline to be subjected to purification treatment, after the centrifugation is finished, the separated solid materials are transferred into a vacuum dryer through the travelling crane to be subjected to drying treatment, and the Rh2 product is.
Claims (9)
- An enzymatic large-scale production system of Rh2, characterized in that the production system comprises:the reaction kettle is provided with a feeding port, a discharging port, a thermometer for measuring the temperature in the kettle, a pH meter for measuring the pH value in the kettle and a stirring device for stirring the materials in the kettle, and the outer wall of the reaction kettle is provided with a jacket layer for circulating cooling water and hot steam;the cooling water circulating device is communicated with the jacket layer of the reaction kettle through a pipeline and is used for providing cooling water for reducing the temperature in the kettle for the jacket layer of the reaction kettle;the hot steam circulating device is communicated with the jacket layer of the reaction kettle through a pipeline and is used for providing hot steam for raising the temperature in the kettle for the jacket layer of the reaction kettle;the acid storage tank is communicated with the interior of the reaction kettle through a pipeline;the alkali storage tank is communicated with the interior of the reaction kettle through a pipeline;the detection device is used for detecting the reaction degree in the reaction kettle;the primary solid-liquid separation device is arranged below a discharge hole of the reaction kettle and is used for performing solid-liquid separation treatment on the material discharged from the reaction kettle;the washing tank comprises a tank body and a stirring device for stirring the materials in the tank body, and is used for washing the materials after primary solid-liquid separation;the secondary solid-liquid separation device is arranged below a discharge hole of the water washing tank and is used for performing solid-liquid separation treatment on the material discharged from the water washing tank;and the drying device is used for drying the materials subjected to the secondary solid-liquid separation.
- 2. The enzymatic large-scale production system of Rh2 of claim 1, wherein: the acid storage tank and the alkali storage tank are arranged above the reaction kettle.
- 3. The enzymatic large-scale production system of Rh2 of claim 1, wherein: the production system further comprises a condensing device used for cooling the solvent heated and volatilized in the kettle of the reaction kettle, wherein the condensing device is arranged above the reaction kettle and communicated with the reaction kettle through a pipeline to form a channel for the cooled solvent to flow back to the kettle.
- 4. The enzymatic large-scale production system of Rh2 of claim 1, wherein: the production system further comprises a transfer device for transferring the material.
- 5. The enzymatic large-scale production system of Rh2 of claim 4, wherein: the transfer device is a travelling crane.
- 6. The enzymatic large-scale production system of Rh2 of claim 1, wherein: the production system further comprises an automatic control system, and the automatic control system comprises a temperature automatic control system for automatically controlling the temperature in the kettle of the reaction kettle to rise and fall and a pH automatic control system for automatically controlling the pH value in the kettle of the reaction kettle to increase and decrease.
- 7. The enzymatic large-scale production system of Rh2 of claim 6, wherein: the temperature automatic control system comprises a temperature reading device, a temperature control unit, a cooling water electromagnetic valve for controlling the cooling water circulating device to open and close, and a hot steam electromagnetic valve for controlling the hot steam circulating device to open and close; one end of the temperature reading device is electrically connected with the thermometer, the other end of the temperature reading device is electrically connected with the temperature control unit, and the temperature control unit respectively controls the cooling water electromagnetic valve and the hot steam electromagnetic valve to be opened and closed.
- 8. The enzymatic large-scale production system of Rh2 of claim 6, wherein: the pH automatic control system comprises a pH reading device, a pH control unit, an acid liquor electromagnetic valve and an alkali liquor electromagnetic valve, wherein the acid liquor electromagnetic valve is used for controlling the acid liquor in the acid storage tank to be conveyed into the reaction kettle and to be stopped, and the alkali liquor electromagnetic valve is used for controlling the alkali liquor in the alkali storage tank to be conveyed into the reaction kettle and to be stopped; one end of the pH reading device is electrically connected with the pH meter, the other end of the pH reading device is electrically connected with the pH control unit, the acid liquor electromagnetic valve is arranged on a pipeline communicated with the acid storage tank and the reaction kettle and close to one end of the reaction kettle, the alkali liquor electromagnetic valve is arranged on a pipeline communicated with the alkali storage tank and the reaction kettle and close to one end of the reaction kettle, and the pH control unit controls the acid liquor electromagnetic valve and the alkali liquor electromagnetic valve to be opened and closed respectively.
- 9. The enzymatic scale-up production system of Rh2 according to any one of claims 1 to 8, wherein: the production system also comprises a sewage treatment system for purifying the liquid after the solid-liquid separation treatment.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2019/086413 WO2020227852A1 (en) | 2019-05-10 | 2019-05-10 | System for large-scale production of rh2 by means of enzymatic method |
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| CN212077054U true CN212077054U (en) | 2020-12-04 |
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| CN201990000212.6U Active CN212077054U (en) | 2019-05-10 | 2019-05-10 | Enzyme method large-scale production system for Rh2 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921306B (en) * | 2009-12-29 | 2013-02-13 | 天津市裕川置业集团有限公司 | System and method for extracting microprotein from sludge through hydrolysis |
| BR102012032807A2 (en) * | 2012-03-30 | 2013-11-19 | Ctc Ct De Tecnologia Canavieira S A | SYSTEM AND PROCESS FOR INTEGRATED ETHANOL PRODUCTION OF FIRST AND SECOND GENERATIONS, AND USE OF INTEGRATION POINTS FOR DITA PRODUCTION |
| WO2014059273A1 (en) * | 2012-10-11 | 2014-04-17 | Butamax Advanced Biofuels Llc | Processes and systems for the production of fermentation products |
| CN104342358B (en) * | 2013-08-07 | 2016-09-21 | 国家电网公司 | Fermentation fuel reactor and the method producing fermentation fuel |
| CN203582868U (en) * | 2013-11-22 | 2014-05-07 | 河南省***（集团）有限公司 | Continuous enzyme hydrolysis device for polypeptides |
| CN203960189U (en) * | 2014-06-20 | 2014-11-26 | 新沂市恒惠淀粉糖有限公司 | Novel amino polypeptide enzyme process production equipment |
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- 2019-05-10 WO PCT/CN2019/086413 patent/WO2020227852A1/en active Application Filing
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