CN211814443U

CN211814443U - Biological cell culture fluid extraction element

Info

Publication number: CN211814443U
Application number: CN202020332358.5U
Authority: CN
Inventors: 刘海洋
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-17
Filing date: 2020-03-17
Publication date: 2020-10-30
Anticipated expiration: 2030-03-17

Abstract

The utility model discloses a biological cell culture solution extraction device, the upper end of a supporting seat is provided with a culture platform, the supporting seat and the culture platform are supported and fixed through a side branch rod, a culture dish is arranged on the culture platform, the upper end of the supporting seat is provided with a test tube platform, the test tube platform is sleeved outside the side branch rod, the test tube platform is connected with the side branch rod in a sliding way, the test tube platform is connected with the side branch rod through a spring, the test tube platform is fixedly provided with a tube sleeve, a reagent tube is inserted on the tube sleeve, a sealing cover is arranged on the reagent tube, one end of the sealing cover is fixed with the reagent tube in a threaded way, the other end of the sealing cover is inserted with a plunger, an elastic separation blade is fixedly arranged in the sealing cover, the elastic separation blade is sealed in an annular way at the inner side of the sealing cover, the upper end of the test tube platform is provided with a liquid guide pipe, the liquid guide pipe is, the utility model discloses isolation protection when being convenient for the culture solution to draw uses the operation swiftly convenient.

Description

Biological cell culture fluid extraction element

Technical Field

The utility model relates to a bioengineering field, concretely relates to biological cell culture solution extraction element.

Background

Bioengineering is an emerging technology which is generally considered to be based on the theory and technology of biology (especially molecular biology, microbiology, genetics, biochemistry and cytology) and combine with modern engineering technologies such as chemical engineering, mechanical engineering and electronic computers, etc., fully utilizes the latest achievements of molecular biology to consciously manipulate genetic materials, directionally modify organisms or functions thereof, create new species with ultra-distant characters in a short time, and then culture such "engineering bacteria" or "engineering cell strains" in a large scale through a proper bioreactor to produce a large amount of useful metabolites or exert unique physiological functions thereof.

In biological cell culture, it is often necessary to extract a sample of a culture solution for various experiments. However, when people extract the culture solution, the culture solution is not isolated from the air, and bacteria as impurities in the air are easy to grow in the culture solution, thereby affecting the quality of the culture solution.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

In order to overcome the defects of the prior art, the biological cell culture solution extraction device is provided, so that the isolation protection is convenient during the extraction of the culture solution, and the use and the operation are quick and convenient.

(II) technical scheme

The utility model discloses a realize through following technical scheme: the utility model provides a biological cell culture solution extraction device, which comprises a supporting seat, wherein the upper end of the supporting seat is provided with a culture table, the supporting seat and the culture table are supported and fixed through a side branch rod, the culture dish is arranged on the culture table, the upper end of the supporting seat is provided with a test tube table, the test tube table is sleeved outside the side branch rod, the test tube table is in sliding connection with the side branch rod, the test tube table is connected with the side branch rod through a spring, the test tube table is fixedly provided with a tube sleeve, a reagent tube is inserted on the tube sleeve, a sealing cover is arranged on the reagent tube, one end of the sealing cover is in threaded fixation with the reagent tube, the other end of the sealing cover is inserted with a plunger, an elastic separation blade is fixedly arranged in the sealing cover, the elastic separation blade is sealed in an annular manner inside the sealing cover, the upper end of the test tube table is provided with a catheter, the catheter is fixed at the, the liquid guide tube is characterized in that a liquid outlet nozzle is arranged at the bottom of the liquid guide tube, the liquid outlet nozzle corresponds to the reagent tube up and down, and a liquid outlet valve is arranged on the liquid outlet nozzle.

Further, a liquid inlet is arranged on the culture dish.

Further, a fastening pad is arranged in the pipe sleeve, and the reagent pipe is tightly matched with the pipe sleeve.

Further, the fastening pad is a rubber pad.

Further, the reagent tube is provided with a metering scale mark.

Further, the elastic blocking piece is a rubber piece.

(III) advantageous effects

Compared with the prior art, the utility model, following beneficial effect has:

1. be equipped with the test tube platform on the supporting seat, the test tube bench is equipped with the pipe box, and the test tube platform passes through spring coupling with the supporting seat, and then the picture peg dismouting of reagent pipe on the pipe box can be realized to the pushing down of test tube platform, and the test tube platform can reset by oneself under the effect of spring after the dismouting, and the reagent pipe of being convenient for is connected with the drain nozzle on the catheter, realizes drawing of culture solution.

2. Be equipped with the closing cap on the reagent pipe, be equipped with the elasticity separation blade in the closing cap, the orificial sealed of reagent pipe when the dismouting removes of being convenient for avoids external dust to get into the intraductal quality that influences the culture solution of reagent. The seal cover is fixed with the reagent tube by screw threads, so that the culture solution extracted from the reagent tube can be poured out conveniently.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic view of the connection structure of the reagent tube and the sealing cover of the present invention.

Fig. 3 is a top view of the inside of the closure of the present invention.

Figure 4 is a schematic view of a catheter structure of the present invention.

1-a support seat; 11-a culture platform; 12-side struts; 13-a culture dish; 14-a liquid inlet; 2-test tube bench; 21-a spring; 22-a pipe sleeve; 23-a reagent tube; 24-a fastening pad; 25-measurement scale lines; 3-sealing the cover; 31-a plunger; 32-an elastic baffle sheet; 4-a catheter; 41-liquid outlet; 42-liquid outlet valve.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The first embodiment:

the biological cell culture solution extraction device shown in fig. 1-4 comprises a support base 1, wherein a culture platform 11 is arranged at the upper end of the support base 1, the support base 1 and the culture platform 11 are supported and fixed through a side support rod 12, a culture dish 13 is arranged on the culture platform 11, a test tube platform 2 is arranged at the upper end of the support base 1, the test tube platform 2 is sleeved outside the side support rod 12, the test tube platform 2 is slidably connected with the side support rod 12, the test tube platform 2 is connected with the side support rod 12 through a spring 21, a tube sleeve 22 is fixedly arranged on the test tube platform 2, a reagent tube 23 is inserted into the tube sleeve 22, a seal cover 3 is arranged on the reagent tube 23, one end of the seal cover 3 is fixed with the reagent tube 23 by screw threads, a plunger 31 is inserted into the other end of the seal cover 3, an elastic baffle 32 is fixedly arranged in the seal cover 3, and the elastic baffle 32 is sealed inside the seal cover 3 in, the upper end of the test tube platform 11 is provided with a liquid guide tube 4, the liquid guide tube 4 is fixed at the bottom of the culture platform 11, the liquid guide tube 4 is connected with a liquid outlet of a culture dish 13, the bottom of the liquid guide tube 4 is provided with a liquid outlet nozzle 41, the liquid outlet nozzle 41 corresponds to the reagent tube 23 up and down, and the liquid outlet nozzle 41 is provided with a liquid outlet valve 42.

Preferably, the culture dish 13 is provided with a liquid inlet 14 for adding the culture solution.

Preferably, a fastening pad 24 is arranged in the pipe sleeve 22, the reagent pipe 23 is tightly matched with the pipe sleeve 22, and the reagent pipe 23 can be stably inserted into the pipe sleeve 22 through the fastening pad 24, so that the culture solution can be conveniently extracted.

Preferably, the reagent tube 23 is provided with a metering scale 25, which facilitates quantitative extraction when the reagent tube 23 measures the culture solution.

Preferably, the elastic flap 32 is a rubber sheet.

Second embodiment:

further, the fastening pad 24 is a rubber pad.

The working principle is as follows: the culture table 11 is provided with a culture dish 13 which can store culture solution, and the culture solution is further conveyed by the liquid guide tube 4 to realize the sampling and extraction of the culture solution.

During extraction, the test tube table 2 can be pressed downwards, the reagent tube 23 is inserted into the tube sleeve 22, and the test tube table 2 is reset to slide upwards under the action of the spring 21, so that the liquid outlet nozzle 41 on the liquid guide tube 4 at the upper end of the test tube table is inserted into the upper end of the tube opening of the reagent tube 23, and the culture solution can be conveniently injected. When the reagent tube 23 is used for inserting and taking liquid, the plunger 31 at the upper end of the reagent tube 23 can be firstly pulled out, when the liquid outlet nozzle 41 on the liquid guide tube 4 is inserted into the reagent tube 23, the lower end of the liquid outlet nozzle 41 extrudes and passes through the elastic baffle 32 and extends into the reagent tube 23, at the moment, the liquid outlet valve 42 on the liquid outlet nozzle 41 is opened to inject the culture liquid, and the metering scale mark 25 on the reagent tube 23 can facilitate the quantitative control of the culture liquid when the culture liquid is injected into the reagent tube 23.

After the culture solution is extracted and injected into the reagent tube 23, the liquid outlet valve 42 can be closed, the test tube table 2 is pressed down, the liquid outlet nozzle 41 is separated from the reagent tube 23, the reagent tube 23 is taken out from the tube sleeve 22, and then the plunger 31 is tightly inserted into the upper end of the sealing cover 3 to realize sealing. In the case of examining the culture solution extracted from the reagent tube 23, the cap 3 may be screwed off, and the culture solution may be poured out for use.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention and are not intended to limit the spirit and scope of the present invention. Without departing from the design concept of the present invention, various modifications and improvements made by the technical solution of the present invention by those skilled in the art should fall into the protection scope of the present invention, and the technical contents claimed by the present invention have been fully recorded in the claims.

Claims

1. A biological cell culture fluid extraction element which characterized in that: comprises a supporting seat (1), a culture platform (11) is arranged at the upper end of the supporting seat (1), the supporting seat (1) and the culture platform (11) are supported and fixed through a side supporting rod (12), a culture dish (13) is arranged on the culture platform (11), a test tube platform (2) is arranged at the upper end of the supporting seat (1), the outer side of the side supporting rod (12) is sleeved with the test tube platform (2), the test tube platform (2) is in sliding connection with the side supporting rod (12), the test tube platform (2) is connected with the side supporting rod (12) through a spring (21), a tube sleeve (22) is fixedly arranged on the test tube platform (2), a reagent tube (23) is inserted on the tube sleeve (22), a sealing cover (3) is arranged on the reagent tube (23), one end of the sealing cover (3) is in threaded fixation with the reagent tube (23), a plunger (31) is inserted at the other end of the sealing cover (3), and an elastic blocking piece (32) is fixedly arranged in the sealing cover (3), elasticity separation blade (32) ring-type seal in the inboard of closing cap (3), the upper end of test tube platform (2) sets up catheter (4), the bottom of cultivateing platform (11) is fixed in catheter (4), catheter (4) are connected with the liquid outlet of culture dish (13), catheter (4) bottom sets up drain nozzle (41), drain nozzle (41) correspond from top to bottom with reagent pipe (23), set up drain valve (42) on drain nozzle (41).

2. The device for extracting a biological cell culture solution according to claim 1, wherein: the culture dish (13) is provided with a liquid inlet (14).

3. The device for extracting a biological cell culture solution according to claim 1, wherein: a fastening pad (24) is arranged in the pipe sleeve (22), and the reagent pipe (23) is tightly matched with the pipe sleeve (22).

4. The device for extracting a biological cell culture solution according to claim 3, wherein: the fastening pad (24) is a rubber pad.

5. The device for extracting a biological cell culture solution according to claim 1, wherein: and metering scale marks (25) are arranged on the reagent tube (23).

6. The device for extracting a biological cell culture solution according to claim 1, wherein: the elastic blocking piece (32) is a rubber piece.