CN210323708U

CN210323708U - Large-area digital PCR reader chip shooting light source box

Info

Publication number: CN210323708U
Application number: CN201921586299.8U
Authority: CN
Inventors: 李云朋
Original assignee: Shanghai Full Gene Biotechnology Co ltd
Current assignee: Shanghai Full Gene Biotechnology Co ltd
Priority date: 2019-09-23
Filing date: 2019-09-23
Publication date: 2020-04-14
Anticipated expiration: 2029-09-23

Abstract

The utility model discloses a large tracts of land digit PCR reader chip shoots light source box, including fixed frame, light source base, fresnel lens, two-way dichroic mirror and reverberation light filter be equipped with the LED light source that is used for providing the shooting light source for digit PCR chip on the light source base, the setting of exciting light filter is in the second cavity, and cuts apart into two independent middle part cavities and shooting cavity with the second cavity, two-way dichroic mirror setting is in shooting the cavity and personally submits 45 with fixed frame's upper and lower side and place set up on fixed frame's the downside and be used for the reverberation via hole of exciting light reflection on the PCR chip. The utility model discloses can satisfy the requirement of the shooting light path condition of jumbo size digital PCR chip, can provide the shooting condition for the digital PCR chip in 2cm 10 ten thousand holes at least, also can satisfy the digital PCR chip of small-size simultaneously and shoot required light path condition.

Description

Large-area digital PCR reader chip shooting light source box

Technical Field

The utility model relates to a digital PCR technical field, in particular to large tracts of land digital PCR reader chip shoots light source box.

Background

Digital PCR is a recent quantitative technique, and nucleic acid quantification based on counting by a single-molecule PCR method is an absolute quantitative method. The method mainly adopts a micro-fluidic or micro-droplet method in the current analytical chemistry hot research field to disperse a large amount of diluted nucleic acid solution into micro-reactors or micro-droplets of a chip, wherein the number of nucleic acid templates in each reactor is less than or equal to 1. Thus, after PCR cycling, a reactor with a nucleic acid molecule template will give a fluorescent signal, and a reactor without a template will have no fluorescent signal. Based on the relative proportions and the volume of the reactor, the nucleic acid concentration of the original solution can be deduced.

In order to realize higher analysis precision, theoretically, the more the micropores of the chip on the digital PCR are, the better the analysis precision is, the more the number of the micropores is, and on the premise that the aperture is basically unchanged, the area of the chip is inevitably increased, the shooting light source box in the prior art can meet the shooting conditions, and great inconvenience is brought to the shooting work of the large-area digital PCR chip.

SUMMERY OF THE UTILITY MODEL

For solving the problem that prior art exists, the utility model aims to provide a reasonable in design, simple structure, can satisfy the requirement of the shooting light path condition of jumbo size digital PCR chip, can provide the shooting condition for the digital PCR chip in 2cm at least 10 ten thousand holes, also can satisfy the large tracts of land digital PCR reader chip that the required light path condition was shot to the small-size digital PCR chip simultaneously and shoot the light source box.

For solving the above technical problem, the utility model discloses a following technical scheme realizes:

a large-area digital PCR reader chip shooting light source box is characterized by comprising

A fixed frame with a hollow interior;

the light source base is arranged on one end of the fixed frame in a sliding manner, and an LED light source for providing a shooting light source for the digital PCR chip is arranged on the light source base;

the Fresnel lens is used for converting scattered light emitted by the LED light source into parallel light with uniform brightness, is arranged in the fixed frame and divides an inner cavity of the fixed frame into two independent first chambers and two independent second chambers;

the excitation light filter is used for filtering parallel light with uniform brightness obtained by the Fresnel lens and obtaining a light source of an excitation light wave band, and the excitation light filter is arranged in the second chamber and divides the second chamber into two independent middle chambers and a shooting chamber;

the dichroic mirror is arranged in the shooting chamber and placed at an angle of 45 degrees with the upper side and the lower side of the fixed frame, and a reflected light via hole for reflecting the exciting light on the PCR chip is formed in the lower side of the fixed frame;

and the reflecting light filter is used for filtering the fluorescent light passing through the dichroic mirror and obtaining the required fluorescent light with a specific wavelength, and is arranged on the filter mounting hole on the upper side surface of the fixed frame.

In a preferred embodiment of the present invention, black light absorbing paper capable of reducing interference of reflected light to imaging is attached to the inner wall of the shooting chamber.

In a preferred embodiment of the present invention, a positioning screw for positioning the light source base is disposed at an upper side of one end of the fixing frame.

In a preferred embodiment of the present invention, the fixing frame is formed by enclosing an upper side plate, a lower side plate, a left side plate, a right side plate, a front side plate and a rear side plate.

Compared with the prior art, the utility model discloses mainly adopt parallel coaxial light source's design theory, produce through high-power monochromatic LED pointolite and shoot required exciting light. The point light source realizes parallel light through the Fresnel lens, and then obtains an excitation light source after noise removal through the excitation light filter, so as to meet the excitation condition of the fluorescent probe on the shooting PCR chip. The exciting light changes the light path direction through the dichroic mirror which forms an angle of 45 degrees with the exciting light, so that the exciting light vertically irradiates the shooting PCR chip, the PCR chip receives the exciting light, the reflected light penetrates through the dichroic mirror, then the reflected light is filtered through the reflecting light filter, the shot camera captures the filtered reflected light, and the requirement on the shooting light path condition of the large-size digital PCR chip is met.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic diagram of the optical path of the present invention.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand and understand, the present invention is further explained by combining with the specific drawings.

Referring to fig. 1 and 2, a large-area digital PCR reader chip light source box is shown, which includes a fixed frame, a light source base 4, a fresnel lens 6, a dichroic mirror 7, and a reflective light filter 8.

Fixed frame's inside is hollow structure, and fixed frame is enclosed to close by last curb plate 13, lower curb plate 10, left side board 5, right side board 11, preceding curb plate 9 and posterior lateral plate 12 and forms, carries out fixed connection through fastening screw 1 between and, adopts this kind of effectual stability can who improves this fixed frame of structure.

The light source base 4 is arranged on the left side plate 5 of the fixed frame in a sliding mode, the LED light source 3 used for providing a shooting light source for the digital PCR chip is arranged on the light source base 4, and the LED light source 3 is fixed on the light source base 4 through screws.

The fresnel lens 6 is used for converting scattered light emitted by the LED light source 3 into parallel light with uniform brightness, and the fresnel lens 6 is disposed in the fixed frame and divides the inner cavity of the fixed frame into two independent first and

second chambers

15 and 23.

The excitation light filter 7 is used for filtering the parallel light with uniform brightness obtained by the Fresnel lens 6 and obtaining a light source of an excitation light wave band, and the excitation light filter 7 is arranged in the second chamber 23 and divides the second chamber 23 into two independent middle chambers 16 and a shooting chamber 17.

The dichroic mirror 8 is used for reflecting the excitation light obtained by the excitation light filter 7 onto the PCR chip 20 and projecting the fluorescence excited from the PCR chip 20, the dichroic mirror 8 is disposed in the imaging chamber 17 and placed at an angle of 45 ° with the upper and

lower side plates

13, 10 of the fixed frame, and the lower side plate 10 of the fixed frame is provided with a reflection light via hole 22 for reflecting the excitation light onto the PCR chip 20.

The reflection light filter 14 is for filtering the fluorescence passing through the dichroic mirror 8 and for obtaining the desired fluorescence of a specific wavelength, and the reflection light filter 14 is mounted on the filter mounting hole 21 on the upper side plate 13 opened with a fixed frame.

The black light absorption paper 18 capable of reducing interference of reflection of light to imaging is attached to the inner wall of the shooting cavity 17, the interference of reflection of light to imaging can be reduced by adopting the structure, and the practicability of the shooting light source box is further improved.

The upper side of the right side plate 11 of the fixed frame is provided with a positioning screw 2 for positioning the light source base 4, the distance between the LED light source 3 and the Fresnel lens 6 is adjusted through the push-pull light source base 4, and after the required position is adjusted, the positioning screw 2 is used for positioning, so that the practicability of the shooting light source box is further improved.

Grooves are formed in the front side plate 9, the rear side plate 12, the upper side plate 13 and the lower side plate 10 according to the size and the thickness of the corresponding optical lenses, and the structure is used for fixing the optical lenses and reducing light leakage.

To sum up the utility model discloses mainly adopt parallel coaxial light source's design theory, produce through high-power monochromatic LED pointolite and shoot required exciting light. The point light source realizes parallel light through the Fresnel lens, and then obtains an excitation light source after noise removal through the excitation light filter, so as to meet the excitation condition of the fluorescent probe on the shooting PCR chip. The exciting light changes the light path direction through the dichroic mirror which forms an angle of 45 degrees with the exciting light, so that the exciting light vertically irradiates the shooting PCR chip, the PCR chip receives the exciting light, the reflected light penetrates through the dichroic mirror, then the reflected light is filtered through the reflecting light filter, the shot camera captures the filtered reflected light, and the requirement on the shooting light path condition of the large-size digital PCR chip is met.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A large-area digital PCR reader chip shooting light source box is characterized by comprising

A fixed frame with a hollow interior;

2. The large area digital PCR reader chip camera light box of claim 1, wherein: and black light absorption paper capable of reducing interference of reflected light on imaging is attached to the inner wall of the shooting chamber.

3. The large area digital PCR reader chip camera light box of claim 1, wherein: and a positioning screw for positioning the light source base is arranged on the upper side of one end of the fixed frame.

4. The large area digital PCR reader chip camera light box of claim 1, wherein: the fixed frame is enclosed by an upper side plate, a lower side plate, a left side plate, a right side plate, a front side plate and a rear side plate.