CN210193829U

CN210193829U - Nucleic acid extraction equipment

Info

Publication number: CN210193829U
Application number: CN201920311623.9U
Authority: CN
Inventors: Hui Chen; 陈辉
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-03-12
Filing date: 2019-03-12
Publication date: 2020-03-27
Anticipated expiration: 2029-03-12

Abstract

The utility model relates to a nucleic acid extraction equipment, including the workstation that has the mesa, still including sliding seat, sample support, reagent strip support, move liquid platform, piston assembly and suction subassembly. The utility model discloses a nucleic acid extraction equipment is including sliding the seat and moving the liquid platform, be provided with sample support and reagent strip support on the seat that slides, and move the liquid bench and be provided with piston assembly and suction subassembly respectively, the sample hole has on the sample support, and the storage tank has on the reagent strip support, the number and the above-mentioned sample hole one-to-one of this storage tank, and hold respectively in each above-mentioned storage tank under having above-mentioned reagent strip state, first suction head hole on each reagent strip, second suction head hole and each reagent hole are relative with the sample hole that corresponds respectively, through moving the liquid platform for reciprocating of the seat that slides or the lateral shifting of the seat relative to moving the liquid platform, can realize loading and sample and the absorption of various reagents of suction head subassembly and suction head, operation such as injection and mixing, thereby realize the automation mechanized operation that nucleic acid drawed.

Description

Nucleic acid extraction equipment

Technical Field

The utility model relates to the field of biotechnology, especially, relate to a nucleic acid extraction equipment.

Background

Nucleic acid extraction is a very important technology in life science research, biotechnology application and gene diagnosis, and early nucleic acid extraction is based on the principle of removing impurities by phenol and chloroform extraction and precipitating nucleic acid by ethanol, but the method is very cumbersome.

At present, the commercial extraction method of nucleic acid is mainly established on a solid phase adsorption method, i.e. a purification method of adsorbing nucleic acid molecules on the surface of a certain solid phase adsorbent under specific conditions (such as pH, specific salt, etc.) and then eluting, and mainly comprises the following two methods: (1) centrifugal column method: filling a silicon-containing material or other solids capable of adsorbing nucleic acid in a centrifugal column as a medium, using chaotropic salt such as guanidinium salt as a binding agent, adsorbing nucleic acid molecules on a solid phase when the nucleic acid molecules pass through the centrifugal column, washing impurities by using a solution containing ethanol, and finally eluting the nucleic acid by using a low-salt solution; the method is simple and convenient, has high extraction efficiency and good extraction effect, is the most used nucleic acid purification method at present, and has the defects of repeated centrifugation in the extraction process and inconvenience for high-throughput and automatic operation. (2) Magnetic bead method: the method comprises the following steps of (1) taking magnetic microspheres coated with silicon materials or other materials capable of combining nucleic acid as solid phase carriers, and separating DNA and RNA from a sample under the action of chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate and the like) and an external magnetic field; the method is easy to realize automatic and high-throughput operation, and has the defects of high cost, time-consuming extraction process, long time-consuming whole gene detection, and incapability of timely obtaining detection results by doctors and patients. Magnetic beads have poor chemical stability. The extraction efficiency is easy to be influenced, and the extraction effect of the magnetic beads of different samples, different manufacturers and different batches is greatly changed. Resulting in unstable extraction and purification results. In addition, cross contamination is easy to occur in the process of repeatedly cleaning the magnetic beads in the magnetic bead extraction process, and false positive results are caused. The conventional automatic nucleic acid extracting apparatus is generally based on the magnetic bead method, such as the nucleic acid extracting apparatus disclosed in the chinese utility model patent No. ZL201620294747.7 (No. CN205635634U), the chinese utility model patent No. ZL201610687392.2 (No. CN106119082A), and the like.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the first technical problem that provides a simple structure, convenient to use's nucleic acid extraction equipment to prior art.

The second technical problem to be solved by the utility model is to provide a nucleic acid extraction equipment that nucleic acid extraction purity is high to prior art.

The third technical problem to be solved by the utility model is to provide a nucleic acid extraction equipment that can not take place cross contamination between nucleic acid extraction in-process sample to prior art.

The utility model provides a technical scheme that above-mentioned technical problem adopted does: a nucleic acid extraction apparatus comprises a worktable with a table top, and is characterized by also comprising

The sliding seat is arranged on the table board and can slide back and forth along the table board under the driving of the sliding component;

the sample support is arranged on the sliding seat, a plurality of sample holes are formed in the sample support in parallel along the direction vertical to the sliding direction of the sliding seat, and the sample holes are used for placing sample tubes;

a reagent strip support arranged on the sliding seat and provided with accommodating grooves for accommodating reagent strips, wherein the number of the accommodating grooves is matched with that of the sample holes

The reagent strips respectively comprise a first sucker hole, a second sucker hole and reagent holes which are linearly arranged, wherein the first sucker hole is used for placing a sucker assembly, the second sucker hole is used for placing suckers, the reagent holes comprise a first reagent hole for containing diluent, a second reagent hole for containing cleaning liquid, a third reagent hole for containing eluent and a fourth reagent hole for storing target substances, the number of the second reagent holes is at least two, and the first sucker hole, the second sucker hole and each reagent hole on each reagent strip are respectively opposite to the corresponding sample holes under the state that the reagent strips are placed in each containing groove;

the liquid-transfering platform is horizontally arranged on a vertical frame fixed on the table-board and can be driven by the lifting component to lift up and down relative to the sliding seat;

the piston assembly is arranged on the liquid transfer table and comprises piston pieces and a piston driving device, the number of the piston pieces is matched with that of the sample holes, each piston piece comprises a piston tube and a piston which is arranged in the piston tube and can move up and down along the inner wall of the piston tube, each piston tube vertically penetrates through the liquid transfer table, a first connector for inserting the suction head is formed at the lower end of each piston tube, and the piston driving device can drive the piston in each piston piece to move up and down relative to the corresponding piston tube; and

the suction assembly comprises connecting suction pipes and a vacuum pump, the number of the connecting suction pipes is matched with that of the sample holes, each connecting suction pipe penetrates through the liquid transfer table, the upper end of each connecting suction pipe is communicated with the vacuum pump,

in order to conveniently heat and preserve heat of the sample according to the operation requirement, the sliding seat is also provided with a heating device for heating the sample support.

In order to conveniently puncture the seal on the reagent strip, the reagent strip puncture device also comprises a puncture assembly for puncturing the seal on each reagent hole, the puncture assembly comprises a puncture frame and a puncture head arranged on the puncture frame, the puncture head is matched with the sample hole, the pistons are all fixed on a piston frame, the piston frame is fixed on an output shaft of a piston driving device, and the puncture frame is fixed with the piston frame. Therefore, when the piston driving device drives the piston frame to move downwards, the piston is driven to move downwards, and simultaneously the puncture frame is driven to move downwards, so that each puncture head can move downwards to puncture a seal on the reagent strip.

In order to better realize the pumping action, the vacuum pumps are in one-to-one correspondence with the piston pieces, the piston frame is provided with pumping joints, the number of the pumping joints is in one-to-one correspondence with the piston pieces, each pumping joint is arranged on the piston frame in a penetrating way, a port at one end of each pumping joint is connected with a port on the corresponding vacuum pump through a connecting pipe, and a port at the other end of each pumping joint is connected with an upper port connected with a suction pipe through a connecting pipe.

In order to ensure that each vacuum pump can be more stably installed, the table top is provided with an installation frame, and each vacuum pump is respectively placed on the installation frame.

For can realize better that the seat that slides and drive down the slip along the mesa under the subassembly that slides, the subassembly that slides includes first slider, first slide rail, briquetting, the motor that slides, hold-in range, driving pulley and driven pulley, above-mentioned slider is fixed in the bottom of the seat that slides, above-mentioned first slide rail is fixed on the mesa, first slider can make a round trip to slide along this first slide rail, the motor that slides is fixed on the mesa, driving pulley fixes on the output shaft of the motor that slides, driven pulley installs on the mesa, the hold-in range tensioning is between driving pulley and driven pulley, above-mentioned briquetting is fixed in one side of the seat that slides and is compressed tightly on the hold-in range. Preferably, the reagent strip comprises a support plate, wherein a plurality of first through holes and second through holes are formed in the support plate in parallel along the length direction of the support plate, tube bodies corresponding to the first through holes in a one-to-one manner extend out of one side of the support plate, sleeve bodies corresponding to the second through holes in a one-to-one manner extend out of one side of the support plate, tube orifices of the tube bodies are respectively opposite to the corresponding first through holes, openings of the sleeve bodies are respectively opposite to the corresponding second through holes, and therefore the first suction head holes, the second suction head holes and the reagent holes are formed respectively.

Furthermore, a first connecting piece and a second connecting piece are respectively arranged on two sides of the carrier plate, and the first connecting piece and the second connecting piece can be detachably connected.

Still further, the first connecting piece is a connecting column, and the second connecting piece is a connecting sleeve into which the connecting column is inserted.

Preferably, the suction head assembly comprises a filter element suction head and a disposable capillary suction pipe, wherein an adsorption filter element is embedded at the lower end of the filter element suction head, the upper end of the disposable capillary suction pipe is sleeved on the lower end of the connecting suction pipe and embedded in the upper end port of the filter element suction head, and the disposable capillary suction pipe is positioned in the filter element suction head and above the adsorption filter element. Through setting up disposable capillary suction pipe, will connect the straw through uncontaminated disposable capillary suction pipe and separate with the filter core suction head, avoid the suction in-process to connect straw and filter core suction head contact to can avoid the filter core suction head to be contaminated, and then can guarantee the purity of nucleic acid extraction, in addition, can make through setting up disposable capillary suction pipe and connect the straw by reuse, thereby reduce the nucleic acid extraction cost. In addition, the disposable capillary pipette enables the solution to be rapidly and completely pumped away from the filter element through the capillary effect, thereby ensuring the thorough separation of the nucleic acid adsorbed on the filter element from the liquid and the thorough cleaning of impurities on the filter element.

In order to realize better suction, the lower end of the disposable capillary suction pipe is vertically opposite to the adsorption filter element.

For promoting the suction effect, the distance between the lower end of the disposable capillary suction tube and the upper surface of the adsorption filter element is 0-10 mm.

Preferably, the distance between the lower end of the disposable capillary suction tube and the upper surface of the adsorption filter element is 2-5mm, so that sufficient suction can be realized.

Preferably, the disposable capillary pipette comprises a first portion at the upper end for fitting over the connecting pipette and a second portion at the lower end having a diameter sized to allow liquid to pass through the disposable capillary pipette smoothly under suction. If the pipe diameter of the second part is too large, the capillary action is not available on the first part, so that the liquid is not beneficial to completely pumping, and part of the liquid is remained between the second part and the filter element suction pipe; on the other hand, if the pipe diameter of the second portion is too small, the liquid may be clogged in the second portion, and smooth suction cannot be achieved.

Further, preferably, the pipe diameter of the second part is 0.5-2.0 mm.

Preferably, the mouth edge of the upper port of the disposable capillary pipette is lower than the mouth edge of the upper port of the filter element suction head. Can realize the secondary through as above design and move back the suction head, move back the suction head for the first time and make filter core suction head and disposable capillary suction pipe break away from, move back the suction head for the second time and make disposable capillary suction pipe and connect the straw to break away from, move back like this behind the suction head filter core suction head can overlap other suction heads on wash the absorption filter core in the filter core suction head to realize elution of nucleic acid material.

In order to facilitate the separation between the disposable capillary suction pipe and the filter element suction head, a convex ring is arranged on the inner circumferential surface of the upper end of the filter element suction head along the circumferential direction, and the convex ring is abutted against the outer circumferential surface of the corresponding position of the disposable capillary suction pipe. Compared with the surface-to-surface contact between the inner peripheral surface of the disposable capillary suction pipe and the outer peripheral surface of the connecting suction pipe, the friction force of the linear-to-surface contact between the convex ring and the outer peripheral surface of the disposable capillary suction pipe is smaller, the design enables the filter element suction head and the disposable capillary suction pipe to be respectively removed from the connecting suction pipe, the filter element suction head with smaller friction force is firstly removed, then the disposable capillary suction pipe is arranged, the arrangement of the convex ring enables the disposable capillary suction pipe and the filter element suction head to form sealing, and in addition, the convex ring can also avoid the adsorption filter element from falling from the filter element suction head.

In order to form better sealing between the disposable capillary suction pipe and the suction head of the filter element, at least two convex rings are arranged up and down.

In order to further promote the sealing between the disposable capillary suction pipe and the suction head of the filter element, the convex rings are arranged at intervals up and down.

Compared with the prior art, the utility model has the advantages of: the utility model discloses a nucleic acid extraction equipment is including sliding the seat and moving the liquid platform, be provided with sample support and reagent strip support on the seat that slides, and move the liquid bench and be provided with piston assembly and suction subassembly respectively, the sample hole has on the sample support, and the storage tank has on the reagent strip support, the number and the above-mentioned sample hole one-to-one of this storage tank, and hold respectively in each above-mentioned storage tank under having above-mentioned reagent strip state, first suction head hole on each reagent strip, second suction head hole and each reagent hole are relative with the sample hole that corresponds respectively, through moving the liquid platform for reciprocating of the seat that slides or the lateral shifting of the seat relative to moving the liquid platform, can realize loading and sample and the absorption of various reagents of suction head subassembly and suction head, operation such as injection and mixing, thereby provide the basis for the automation mechanized operation of nucleic acid extraction. Furthermore, the utility model discloses in through setting up disposable capillary straw to can avoid the suction in-process to connect the lower extreme that the straw was connected in the straw contact and pollute and connect the straw, thereby can guarantee that different nucleic acid draws and can not produce the pollution between the sample, and can realize connecting the used repeatedly of straw.

Drawings

FIG. 1 is a schematic structural view of a nucleic acid extraction apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic view of the structure of FIG. 1 in another direction;

FIG. 3 is a schematic view of a partial structure of a nucleic acid isolation apparatus according to an embodiment of the present invention;

FIG. 4 is an enlarged view of portion I of FIG. 3;

FIG. 5 is a schematic view of the structure of FIG. 3 in another direction;

FIG. 6 is a schematic view of the structure of FIG. 3 in still another direction;

FIG. 7 is a schematic view of another partial structure of a nucleic acid isolation apparatus according to an embodiment of the present invention;

FIG. 8 is a schematic view of still another partial structure of a nucleic acid isolation apparatus according to an embodiment of the present invention;

fig. 9 is a schematic structural view of the head withdrawing plate in the embodiment of the present invention;

FIG. 10 is a schematic view of a reagent strip holder according to an embodiment of the present invention;

fig. 11 is a schematic structural view of a sample holder according to an embodiment of the present invention;

fig. 12 is an exploded view of the piston member according to an embodiment of the present invention;

FIG. 13 is a schematic structural view of a reagent strip according to an embodiment of the present invention;

FIG. 14 is a schematic view of the structure of FIG. 13 in another direction;

FIG. 15 is a schematic view of the structure of FIG. 13 in still another orientation;

fig. 16 is a schematic structural view of a suction head assembly according to an embodiment of the present invention;

figure 17 is a cross-sectional view of a suction head assembly in an embodiment of the present invention;

FIG. 18 is an enlarged view of section II of FIG. 17;

FIG. 19 is a schematic view of a filter head according to an embodiment of the present invention;

fig. 20 is an exploded view of a suction head assembly according to an embodiment of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments.

As shown in FIGS. 1 to 20, a nucleic acid extraction apparatus includes a table 1 having a table top 10, a slide base 21, a sample holder 3, a reagent strip holder 213, a pipetting table 4, a piston assembly, and a pumping assembly. In this embodiment, the top 10 is provided with a housing 11, the slide base 21 and the pipetting table 4 are both provided in the housing 11, and a cooling fan 13 is provided on the back of the housing 11.

The sliding seat 21 is disposed on the table top 10 and can slide back and forth along the table top 10 under the driving of the sliding assembly 22. In this embodiment, in order to better realize the sliding of the sliding seat 21 along the table top 10 under the driving of the sliding assembly 22, the sliding assembly 22 includes a first slider 227, a first slide rail 226, a pressing block 225, a sliding motor 221, a synchronous belt 224, a driving pulley 222, and a driven pulley 223, the first slider 227 is fixed at the bottom of the sliding seat 21, the first slide rail 226 is fixed on the table top 10, the first slider 227 can slide back and forth along the first slide rail 226, the sliding motor 221 is fixed on the table top 10, the driving pulley 222 is fixed on an output shaft of the sliding motor 221, the driven pulley 223 is installed on the table top 10, the synchronous belt 224 is tensioned between the driving pulley 222 and the driven pulley 223, and the pressing block 225 is fixed at one side of the sliding seat 21 and pressed on the synchronous belt 224. In this embodiment, the sliding motor 221 is a stepping motor.

The sample holder 3 is disposed on the sliding seat 21, the sample holder 3 has a plurality of sample holes 31 arranged in parallel in a direction perpendicular to the sliding direction of the sliding seat 21, and the sample holes 31 are used for placing sample tubes 15. In this embodiment, the sample holder 3 is in a long block shape, the sample holes 31 are uniformly distributed along the length direction, and each sample hole 31 extends along the height direction of the sample holder 3 to form a blind hole shape. In order to conveniently heat and preserve the temperature of the sample according to the operation requirement, a heating device for heating the sample tube 15 placed in the sample hole 31 is also arranged on the sliding seat 21. Specifically, in this embodiment, the heating device is a heating pool 211 disposed at the front end of the sliding seat 21, and the sample holder 3 is accommodated in the heating pool 211, so that the sample on the sample holder 3 can be heated and insulated sufficiently.

The reagent strip holder 213 is disposed at the rear end of the slide seat 21, and is provided with accommodating grooves 213a for accommodating reagent strips 7, the number of the accommodating grooves 213a of the reagent strips 7 corresponds to one of the sample holes 31, the reagent strips 7 each include a first pipette tip hole 71, second

pipette tip holes

72a, 72b, and reagent holes linearly disposed, the first pipette tip hole 71 is used for accommodating a pipette tip assembly, the second

pipette tip holes

72a, 72b are used for accommodating pipette tips 82, the reagent holes include a first reagent hole 73 for accommodating a diluent,

second reagent holes

74a, 74b, 74c, 74d for accommodating a cleaning solution, a third reagent hole 75 for accommodating an eluent, and a fourth reagent hole 76 for storing a target substance, the first pipette tip hole 71 and the second pipette tip hole 72a of each reagent strip 7 are disposed in the

accommodating grooves

213a, 72b and each reagent well face the corresponding sample well 31. In this embodiment, the reagent strip 7 includes a carrier 70, a plurality of first through holes 701 and second through holes 702 are disposed on the carrier 70 in parallel along a length direction thereof, a tube body 703 corresponding to the first through holes 701 extends from one side of the carrier 70, a sleeve body 704 corresponding to the second through holes 702 extends from one side of the carrier 70, and a tube opening of each tube body 703 faces the corresponding first through hole 701 respectively, and an opening of each sleeve body 704 faces the corresponding second through hole 702 respectively, so as to form the first pipette tip hole 71, the second

pipette tip holes

72a, 72b, and each reagent hole respectively. Furthermore, the

second reagent wells

74a, 74b, 74c, and 74d contain cleaning solutions, which are a first cleaning solution, a second cleaning solution, a third cleaning solution, and a fourth cleaning solution, respectively, so that the quality of nucleic acid extraction can be effectively ensured through multiple rinses during the nucleic acid extraction process.

Still further, the carrier plate 70 is provided with a first connecting member 78 and a second connecting member 77 at two sides thereof, respectively, and the first connecting member 78 and the second connecting member 77 can be detachably connected. Two or more reagent strips 7 can be connected through the first connecting piece 78 and the second connecting piece 77, so that the reagent strips 7 after being connected and combined can be vertically placed vertically, a device is not required to be additionally arranged to realize the placement of the reagent strips 7, and the use of the reagent strips 7 is facilitated. The detachable connection between the first connecting member 78 and the second connecting member 77 can be realized in various specific ways, and preferably, the first connecting member 78 is a connecting column, and the second connecting member 77 is a connecting sleeve for inserting the connecting column. Specifically, the first connecting member 78 is fixed at the bottom of one side edge of the carrier plate 70, the first connecting member 78 extends vertically downward and is suspended, the second connecting member 77 is fixed at the bottom of the other side edge of the carrier plate 70 and/or the corresponding tube wall of the corresponding tube 703, and the second connecting member 77 extends vertically downward and has an opening exposed outside.

The liquid transfer table 4 is horizontally disposed on a vertical stand 12 fixed to the top 10 and can be moved up and down with respect to the slide base 21 by an elevating unit 64. In order to achieve the up-and-down movement of the liquid-transferring platform 4 driven by the lifting assembly 64, the lifting assembly 64 includes a lifting motor 641, a lifting screw 642 and a lifting seat 643, the upright frame 12 includes a door frame 121 and a vertical plate 122 fixed on the top plate of the door frame 121, the lifting motor 641 is installed on the upper end of the vertical plate 122, the vertical plate 122 is provided with a second slide rail 122b and a slide hole 122a extending vertically below the lifting motor 641, the lifting screw 642 is vertically arranged and has its upper end connected with the output shaft of the lifting motor 641 and its lower end connected with the top plate of the door frame 121, the lifting seat 643 is arranged on the vertical plate 122 through the slide hole 122a and has its front end fixed with the liquid-transferring platform 4, its rear end sleeved on the screw 642 and forms a screw transmission pair with the screw 642, and the lifting seat 643 is provided with a second slide block 644, when the lifting motor drives the screw 642 to rotate, the screw rod 642 can drive the lifting seat 643, and enable the second slider 644 to slide up and down along the second slide rail 122b, so that the corresponding position of the lifting seat 643 can move up and down along the slide hole 122 a. In the initial state, the reagent strip holder 231 is positioned below the gate frame 121.

The piston assembly is disposed on the pipetting platform 4, and includes piston members 62 and a piston driving device 61, in this embodiment, the piston driving device 61 is a motor, the number of the piston members 62 corresponds to the number of the sample wells 31 one by one, each piston member 62 includes a piston tube 622 and a piston 621 which is disposed in the piston tube 622 and can move up and down along the inner wall of the piston tube 622, each piston tube 622 vertically penetrates the pipetting platform 4, a first joint 621a for inserting the pipette tip 82 is formed at the lower end of the piston tube 622, and the piston driving device 61 can drive the piston 621 of each piston member 62 to move up and down relative to the corresponding piston tube 622. In this embodiment, the piston driving device 61 is a through stepping motor, the pistons 621 are fixed on the piston frame 63, each piston 621 includes a piston body 6210 and a piston rod 6211 fixed at the top end of the piston body 6210, and the piston frame 63 is fixed on the output shaft of the piston driving device 61. In order to mount the vacuum pumps 5 more stably, the table 10 is provided with a mounting frame 51, and the vacuum pumps 5 are respectively placed on the mounting frame 51.

The suction assembly comprises connecting suction pipes 91 and a vacuum pump 5, the number of the connecting suction pipes 91 corresponds to the number of the sample holes 31 one by one, each connecting suction pipe 91 penetrates through the liquid transfer table 4, the upper end of each connecting suction pipe 91 is communicated with the vacuum pump 5, and the lower end of each connecting suction pipe 91 is provided with a second connector 911 for connecting the suction head assembly in an inserting manner. In order to better perform the suction operation, the vacuum pumps 5 are provided in plurality and correspond to the connection suction pipes 91 one by one, the piston frame 63 is provided with suction connectors 92, the number of the suction connectors 92 corresponds to the number of the connection suction pipes 91 one by one, each suction connector 92 is inserted into the suction connector frame 65, one end interface of each suction connector 92 is connected to an interface of the corresponding vacuum pump 5 through a connection pipe (not shown), and the other end interface is connected to an upper end interface of the connection suction pipe 91 through a connection pipe (not shown).

The suction head assembly includes a cartridge suction head 83 and a disposable capillary suction tube 81, wherein a lower port of the connection suction tube 91 is inserted into an upper end of the cartridge suction head 83, and an adsorption cartridge 830 is inserted into a lower end of the cartridge suction head 83. The upper end of the disposable capillary suction tube 81 is fitted over the lower end of the connection suction tube 91 and fitted into the upper end of the suction head 83, the disposable capillary suction tube 81 is positioned in the suction head 83 above the adsorption filter element 830, and the lower end of the disposable capillary suction tube 81 is vertically opposite to the adsorption filter element 830. Thus, in this embodiment, the disposable capillary pipette 81 can prevent the connection pipette 91 from contacting the filter element pipette head 83 during the aspiration process, thereby preventing the filter element pipette head 83 from being contaminated and ensuring the purity of nucleic acid extraction.

Further, the lower end of the disposable capillary suction tube 81 is vertically opposite to the adsorption filter 830, and the lower end of the disposable capillary suction tube 81 is spaced from the upper surface of the adsorption filter 830 by a suitable distance to maximize the suction effect. In this embodiment, in order to achieve sufficient suction, the distance between the lower end of the disposable capillary suction tube 81 and the upper surface of the adsorption filter element 830 is 4 mm.

The disposable capillary pipette 81 includes a first portion 811 at the upper end and a second portion 812 at the lower end, wherein the first portion 811 is adapted to be coupled to the connection pipette 91, and the second portion 812 has a diameter that allows the liquid to smoothly pass through the disposable capillary pipette 81 under the suction force. If the pipe diameter of the second portion 812 is too large, the capillary action of the second portion 812 is insignificant, and the liquid remains between the second portion 812 and the connection straw 91, whereas if the pipe diameter of the second portion 812 is too small, the liquid is blocked in the second portion 812, and thus smooth suction cannot be achieved. In this embodiment, preferably, the pipe diameter of the pipe body of the second portion 812 is 0.5 to 2.0 mm.

Still further, to realize the secondary withdrawal of the suction head, the edge of the upper port of the disposable capillary suction tube 81 is lower than the edge of the upper port of the above-mentioned cartridge suction head 83. Thus, the first suction head withdrawal enables the disposable capillary suction pipe 81 and the filter element suction head 83 to be separated, the second suction head withdrawal enables the disposable capillary suction pipe 81 and the connecting suction pipe 91 to be separated, and the filter element suction head 83 can be sleeved on other suction heads to clean the adsorption filter element 830 in the filter element suction head 83 after the suction heads are withdrawn, so that elution of nucleic acid materials is realized.

In addition, in order to facilitate the separation between the disposable capillary suction tube 81 and the filter element suction head 83, a convex ring 831 is provided along the circumferential direction on the inner circumferential surface of the upper end of the filter element suction head 83, and the convex ring 831 abuts against the outer circumferential surface of the corresponding portion of the disposable capillary suction tube 81. The frictional force of the line-surface contact between the collar 831 and the outer circumferential surface of the disposable capillary suction tube 81 is smaller than the surface-surface contact between the outer circumferential surface of the disposable capillary suction tube 81 and the inner circumferential surface of the cartridge suction tube 83, and this design allows the cartridge suction tube 83 and the disposable capillary suction tube 81 to be removed from the connection suction tube 91, respectively. The filter element suction head 83 with low friction is removed first, and then the disposable capillary suction tube 81, and the arrangement of the convex ring 831 enables a seal to be formed between the disposable capillary suction tube 81 and the filter element suction head 83. In this embodiment, in order to form a better seal between the disposable capillary suction tube 81 and the filter element suction head 83, the two protruding rings 831 are vertically disposed, and the two protruding rings 831 are vertically spaced from each other, so that the seal between the secondary tube and the filter element suction head 83 can be further promoted, and the design of the protruding rings 831 can also prevent the adsorption filter element 830 from falling off from the filter element suction head 83.

For can conveniently puncture the seal on the reagent strip 7, the utility model provides a nucleic acid extraction equipment is still including the puncture subassembly that is used for puncturing the seal on each reagent hole, should puncture the subassembly including puncture frame 141 and install puncture head 14 on puncture frame 141, should puncture head 14 and above-mentioned sample hole 31 one-to-one, and above-mentioned puncture frame 141 is fixed as an organic whole with above-mentioned piston frame 63. Thus, when the lift motor 641 drives the pipetting table 4 to move down, and the piston driving device 61 drives the piston rack 63 to move down to drive the piston 621 to move down, the puncture rack 141 is driven to move down, and each puncture head 14 can move down to puncture the seal on each reagent hole of the reagent strip 7. In order to facilitate the unloading of the suction head assemblies on the suction head 82 on the first joint 621a and the second joint 911, a head retreating plate 41 is fixed at the bottom of the pipetting platform 4, a first head retreating hole 41a and a second head retreating hole 41b are respectively distributed on the head retreating plate 41, the first joint 621a and the second joint 911 are respectively arranged in the first head retreating hole 41a and the second head retreating hole 41b in a penetrating manner, the aperture of the first head retreating hole 41a is matched with the radius of the corresponding part of the first joint 621a and is smaller than the radius of the connecting end of the suction head 83 of the filter element, and the aperture of the second head retreating hole 41b is matched with the radius of the corresponding part of the second joint 911 and is smaller than the radius of the connecting end of the suction head. Guide columns 411 are respectively vertically arranged at two ends of the pipetting platform 4, and when the piston driving device 61 drives the piston rack 63 to move downwards to a certain distance, the piston rack 63 can be abutted against the top ends of the guide columns 411. Each guide column 411 is all worn to establish on pipetting platform 4 and can reciprocate for pipetting platform 4, and its lower extreme is fixed with above-mentioned head board 41 that moves back respectively, and each guide column 411 is the thick structure in the middle of both ends, and the middle part of each guide column 411 is all worn to be equipped with reset spring 411 a. Specifically, the lower end of each guide column 411 is inserted into the pipetting table 4, and the return spring 411a is limited between the upper end of the guide column 411 and the top surface of the pipetting table 4, when the piston driving device 61 drives the piston rack 63 to move downwards to a certain distance, the piston rack 63 abuts against the top end of each guide column 411, so as to drive the head retreating plate 41 to move downwards to realize the retreating of the suction head assembly or the pipette head 82, when the pressure acting on each guide column 411 disappears, each guide column 411 is reset under the action of the corresponding return spring 411 a. Thus, by providing the above-described back plate 41, it is possible to unload the suction head 82 from the first connector 621a, or the suction head assembly from the second connector 911, or the cartridge suction head 83 from the disposable capillary suction tube 81.

The working process of the utility model is as follows:

the sample tube 15 and the reagent strip 7 are respectively placed in the sample support 3 and the reagent strip support 231, the nucleic acid extraction equipment is started, the motors are reset, and the heating device heats and preserves the temperature of the sample according to a set heat preservation program. After the sample is insulated, the piston driving device 61 cooperates with the lifting motor 641 to drive the puncture rack 141, so that each puncture head 14 punctures the seal on the corresponding reagent hole, and the preparation is made for the subsequent operation.

The slide motor 221 drives the slide base 21 so that the tips 82 on the slide base 21 are aligned with the first connectors 621a on the liquid-transferring platform 4, and the lift motor 641 drives the liquid-transferring platform 4 to move down so that the first connectors 621a press and lift the tips 82. The slide motor 221 drives the slide base 21 so that the first reagent wells 73 containing the diluent are aligned with the pipette tips 82 one by one, the pipette table 4 is lowered, the pipette tips 82 are inserted into the corresponding first reagent wells 73, the piston driving device 61 is operated, the pipette tips 82 suck the diluent, and after the suction is completed, the lift motor 641 drives the pipette table 4 to be raised.

The sliding seat 21 translates to enable each sample tube 15 on the sample support 3 to be opposite to each first connector 621a on the liquid transfer table 4 one by one, the lifting motor 641 drives the liquid transfer table 4 to move downwards, the piston driving device 61 works to add the diluent in each suction head 82 into the corresponding sample tube 15, and the piston piece 62 performs suction for several times to enable the diluent and the sample to be fully mixed and to keep warm for 5-10 minutes as required.

The lift motor 641 drives the pipetting platform 4 to ascend, the slide motor 221 enables the second

pipette tip holes

72a and 72b to face the first connectors 621a one by one, the pipetting platform 4 descends, the piston driving device 61 works and drives the head withdrawing plate 41 to withdraw the pipette tips 82 into the corresponding second

pipette tip holes

72a and 72 b. The liquid transfer table 4 is lifted, the slide seat 21 moves, the disposable capillary pipettes 81 are respectively opposite to the second connectors 911 one by one, the liquid transfer table 4 is lowered, and the suction head assembly is pressed and lifted.

The sliding seat 21 moves to enable the sample tube 15 to be positioned below the second connector 911, the liquid transferring platform 4 descends, the vacuum pump 5 works, and a sample is extracted. The liquid transfer table 4 is raised, the slide base 21 is moved to position the first cleaning liquid below the second joint 911, the liquid transfer table 4 is lowered, and the vacuum pump 5 is operated to pump the first cleaning liquid. The liquid-transferring table 4 is raised, the slide base 21 is moved to position the second cleaning liquid below the second connector 911, the liquid-transferring table 4 is lowered, the vacuum pump 5 is operated, and the second cleaning liquid is extracted. The liquid transfer table 4 is raised, the slide base 21 is moved to position the third cleaning liquid below the second connector 911, the liquid transfer table 4 is lowered, and the vacuum pump 5 is operated to draw the third cleaning liquid. The liquid transferring platform 4 ascends, the sliding seat 21 moves to enable the fourth cleaning solution to be positioned below the second connector 911, the liquid transferring platform 4 descends, the vacuum pump 5 works, and the fourth cleaning solution is sucked and drained for 2-5 minutes by air suction at the same time to suck the filter element sucker 83.

The liquid-transfering platform 4 is lifted, the sliding seat 21 moves to enable the first suction head hole 71 to be positioned below the second connector 911, the liquid-transfering platform 4 is lowered, the piston driving device 61 works, and the head withdrawing plate 41 is driven to withdraw the filter element suction head 83 to the first suction head hole 71. The pipetting platform 4 is lifted, the sliding seat 21 moves and makes the first reagent hole 73 positioned below the second connector 911, the pipetting platform 4 is lowered, the piston driving device 61 works and drives the retreating plate 41 to retreat the disposable capillary pipette 81 into the first reagent hole 73 (the diluent in the first reagent hole is evacuated and is used for placing the disposable capillary pipette 81 after use). The liquid transfer table 4 is raised, the slide base 21 is moved to position the filter element tip 83 below the first joint 621a, and the liquid transfer table 4 is lowered to press and raise the filter element tip 83. The slide holder 21 moves to position the third reagent well 75 below the first connector 621a, the pipette table 4 is lowered, the piston driving device 61 is operated, and the eluent is sucked.

After the completion of the pipetting, the pipette table 4 is raised, the slide base 21 is moved so that the fourth reagent well 76 is positioned below the first connector 621a, the pipette table 4 is lowered, and the piston driving device 61 is operated to push out the eluent.

The pipetting platform 4 is ascended, the slide motor 221 drives the slide base 21 to enable the first suction head hole 71 to be positioned below the first connector 621a, the pipetting platform 4 descends, the piston driving device 61 works and drives the head withdrawing plate 41 to withdraw the filter element suction head 83 into the first suction head hole 71, and each motor is reset.

Compared with the prior art, the utility model provides a nucleic acid extraction equipment can realize the automatic fast operation of nucleic acid extraction, and whole process only needs 5 ~ 10 minutes, and the short time can not realize the processing of large sample volume to realize the high-efficient extraction of nucleic acid, and adopt suction abluent mode purification sample, and then can obtain the nucleic acid material of high purity, and no cross contamination risk, good reproducibility, with low costs.

Claims

1. A nucleic acid extraction apparatus comprising a table (1) having a table top (10), characterized by further comprising

The sliding seat (21) is arranged on the table top (10) and can slide back and forth along the table top (10) under the driving of the sliding component (22);

the sample support (3) is arranged on the sliding seat (21), the sample support (3) is provided with a plurality of sample holes (31) which are arranged in parallel along the direction vertical to the sliding direction of the sliding seat (21), and the sample holes (31) are used for placing sample tubes (15);

a reagent strip holder (213) disposed on the sliding seat (21), wherein a receiving groove (213a) for receiving a reagent strip (7) is disposed on the reagent strip holder, the number of the receiving grooves (213a) matches the number of the sample holes (31), each reagent strip (7) comprises a first pipette tip hole (71), a second pipette tip hole (72a, 72b) and a reagent hole, wherein the first pipette tip hole (71) is used for receiving a pipette tip assembly, the second pipette tip hole (72a, 72b) is used for receiving a pipette tip (82), the reagent holes comprise a first reagent hole (73) for receiving a diluent, a second reagent hole (74a, 74b, 74c, 74d) for receiving a cleaning solution, a third reagent hole (75) for receiving an eluent and a fourth reagent hole (76) for receiving a target substance, and the reagent strips (7) are respectively received in the receiving grooves (213a), the first sucker hole (71), the second sucker holes (72a, 72b) and each reagent hole on each reagent strip (7) are respectively opposite to the corresponding sample hole (31);

a liquid-transfering platform (4) which is horizontally arranged on a vertical frame (12) fixed on the table top (10) and can be driven by a lifting component (64) to lift up and down relative to the sliding seat (21);

the piston assembly is arranged on the pipetting platform (4) and comprises piston pieces (62) and a piston driving device (61), the number of the piston pieces (62) is matched with that of the sample holes (31), each piston piece (62) comprises a piston tube (622) and a piston (621) which is arranged in the piston tube (622) and can move up and down along the inner wall of the piston tube (622), each piston tube (622) vertically penetrates through the pipetting platform (4), a first connector (621a) used for inserting and connecting the pipette tips (82) is formed at the lower end of each piston tube (622), and the piston driving device (61) can drive the piston (621) in each piston piece (62) to move up and down relative to the corresponding piston tube (622); and

the suction assembly comprises connecting suction pipes (91) and a vacuum pump (5), the number of the connecting suction pipes (91) is matched with that of the sample holes (31), the connecting suction pipes (91) are arranged on the liquid transfer table (4) in a penetrating mode, the upper ends of the connecting suction pipes (91) are communicated with the vacuum pump (5) respectively, and second connectors (911) used for being connected with the suction head assembly in an inserting mode are formed at the lower ends of the connecting suction pipes respectively.

2. The nucleic acid extraction apparatus according to claim 1, wherein a heating device for heating the sample holder (3) is further provided on the slide base (21).

3. The nucleic acid extraction apparatus according to claim 1 or 2, further comprising a piercing assembly for piercing a seal on each reagent well, the piercing assembly comprising a piercing rack (141) and a piercing head (14) mounted on the piercing rack (141), the piercing head (14) being mated with the sample well (31),

the pistons 621 are each mounted on a piston frame 63, the piston frame 63 is fixed to an output shaft of a piston driving device 61, and the puncture frame 141 is fixed to the piston frame 63.

4. The nucleic acid isolation apparatus according to claim 3, wherein the vacuum pumps (5) are provided in plurality and correspond to the piston members (62) one by one, the piston holder (63) is provided with suction joints (92), the number of the suction joints (92) corresponds to one by one to the piston members (62), each suction joint (92) is inserted into the suction joint holder (65), one end port of each suction joint (92) is connected to the port of the corresponding vacuum pump (5) by a connection pipe, and the other end port is connected to the upper end port of the connection pipette (91) by a connection pipe.

5. The nucleic acid extraction apparatus according to claim 4, wherein a mounting frame (51) is provided on the stage (10), and each vacuum pump (5) is placed on the mounting frame (51), respectively.

6. The nucleic acid extraction apparatus according to claim 1 or 2, wherein the slide assembly (22) comprises a first slider (227), a first slide rail (226), a pressure block (225), a slide motor (221), a timing belt (224), a driving pulley (222), and a driven pulley (223), the first sliding block (227) is fixed at the bottom of the sliding seat (21), the first sliding rail (226) is fixed on the table top (10), the first sliding block (227) can slide back and forth along the first sliding rail (226), the sliding motor (221) is fixed on the table top (10), the driving pulley (222) is fixed on the output shaft of the sliding motor (221), the driven pulley (223) is installed on the table top (10), the synchronous belt (224) is tensioned between the driving pulley (222) and the driven pulley (223), the pressing block (225) is fixed on one side of the sliding seat (21) and is pressed on the synchronous belt (224).

7. The nucleic acid extraction apparatus according to claim 1 or 2, wherein the lifting assembly (64) comprises a lifting motor (641), a lifting screw (642) and a lifting seat (643), the stand (12) comprises a door-shaped frame (121) and a vertical plate (122) fixed on the top plate of the door-shaped frame (121), the lifting motor (641) is installed at the upper end of the vertical plate (122), the vertical plate (122) is provided with a second slide rail (122b) and a slide hole (122a) which extend vertically below the lifting motor (641), the lifting screw (642) is vertically arranged, the upper end of the lifting screw is connected with the output shaft of the lifting motor (641), the lower end of the lifting screw is connected with the top plate of the door-shaped frame (121), the lifting seat (643) is arranged on the vertical plate (122) through the slide hole (122a), the front end of the lifting seat is fixed with the liquid-transferring table (4), the rear end of the lifting seat is sleeved on the screw (642) and forms a screw transmission pair with the liquid-transferring table (642), and the lifting seat (643) is provided with a second sliding block (644),

when the lifting motor (641) drives the screw rod (642) to rotate, the screw rod (642) can drive the lifting seat (643), and the second sliding block (644) can slide up and down along the second sliding rail (122b), so that the corresponding position of the lifting seat (643) can move up and down along the sliding hole (122 a).

8. The nucleic acid extraction apparatus according to claim 1 or 2, wherein the reagent strip (7) includes a support plate (70), the support plate (70) is provided with a plurality of first through holes (701) and second through holes (702) in parallel along a length direction thereof, tubes (703) corresponding to the first through holes (701) extend from one side of the support plate (70), sleeves (704) corresponding to the second through holes (702) extend from one side of the support plate (70), and a nozzle of each tube (703) faces the corresponding first through hole (701) and an opening of each sleeve (704) faces the corresponding second through hole (702), so as to form the first pipette tip hole (71), the second pipette tip holes (72a, 72b), and the reagent holes, respectively.

9. The nucleic acid extracting apparatus according to claim 8, wherein the carrier plate (70) is provided at both sides thereof with a first connecting member (78) and a second connecting member (77), respectively, and the first connecting member (78) and the second connecting member (77) are detachably connectable.

10. The nucleic acid extraction apparatus according to claim 9, wherein the first connector (78) is a connecting column, and the second connector (77) is a connecting sleeve into which the connecting column is inserted.

11. The nucleic acid extraction apparatus according to claim 1 or 2, wherein the suction head assembly comprises a filter element suction head (83) and a disposable capillary pipette (81), an adsorption filter element (830) is embedded in the filter element suction head (83), an upper end of the disposable capillary pipette (81) is fitted over a lower end of the connection pipette (91) and is embedded in an upper port of the filter element suction head (83), and the disposable capillary pipette (81) is located in the filter element suction head (83) and above the adsorption filter element (830).

12. The nucleic acid isolation apparatus according to claim 11, wherein the lower end of the disposable capillary pipette (81) is vertically opposed to the adsorption cartridge (830).

13. The nucleic acid isolation apparatus according to claim 12, wherein the distance between the lower end of the disposable capillary pipette (81) and the upper side of the adsorption filter element (830) is 0 to 10 mm.

14. The nucleic acid extraction apparatus according to claim 13, wherein the distance between the lower end of the disposable capillary pipette (81) and the upper surface of the adsorption cartridge (830) is 2 to 5 mm.

15. The nucleic acid extraction apparatus according to claim 11, wherein the disposable capillary pipette (81) comprises a first portion (811) at an upper end and a second portion (812) at a lower end, wherein the first portion (811) is adapted to be fitted around the connection pipette (91), and the second portion (812) has a diameter sized to allow liquid to smoothly pass through the disposable capillary pipette (81) by suction.

16. The nucleic acid extraction apparatus according to claim 15, wherein the tube diameter of the first portion (811) is 0.5 to 2.0 mm.

17. The nucleic acid extraction apparatus according to claim 11, wherein the mouth edge of the upper port of the disposable capillary pipette (81) is lower than the mouth edge of the upper port of the suction head (83) of the filter cartridge.

18. The nucleic acid isolation apparatus according to claim 11, wherein a protruding ring (831) is provided on an inner peripheral surface of an upper end of the cartridge tip (83) in a circumferential direction, the protruding ring (831) being capable of abutting against an outer peripheral surface of a corresponding portion of the disposable capillary pipette (81).

19. The nucleic acid extraction apparatus according to claim 18, wherein the protruding rings (831) are at least two disposed vertically, and each of the protruding rings (831) is disposed vertically at an interval.