CN201624028U - Device for researching plant root exudate allelopathy - Google Patents

Device for researching plant root exudate allelopathy Download PDF

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Publication number
CN201624028U
CN201624028U CN2009202906000U CN200920290600U CN201624028U CN 201624028 U CN201624028 U CN 201624028U CN 2009202906000 U CN2009202906000 U CN 2009202906000U CN 200920290600 U CN200920290600 U CN 200920290600U CN 201624028 U CN201624028 U CN 201624028U
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China
Prior art keywords
culturing pot
allelopathy
plant
researching
plant culturing
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Expired - Fee Related
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CN2009202906000U
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Chinese (zh)
Inventor
刘苹
于淑芳
赵海军
杨力
江丽华
田叶
魏建林
王梅
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Soil and Fertilizer Institute of Shandong Academy of Agricultural Sciences
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Soil and Fertilizer Institute of Shandong Academy of Agricultural Sciences
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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Abstract

The utility model relates to a device for researching plant root exudate allelopathy, which comprises a donor plant culture bowl, a recipient plant culture bowl, communicating pipes and a valve. The bottom part of the donor plant culture bowl is communicated to an upper opening of the recipient plant culture bowl through the communicating pipe with the valve; and the bottom part of the recipient plant culture bowl is communicated to an upper opening of the donor plant culture bowl through the communicating pipe with an inflation port in another pipeline. The utility model solves the problems of a conventional device and a method like long time consumption, heavy workload and low precision.

Description

Device for researching plant root exudate allelopathy
Technical field
The utility model relates to the device of a kind of secretions from plant roots to surrounding plants (comprising individuality of the same race) STUDY ON ALLELOPATHIC EFFECTS, platymiscium science and field of ecology.
Background technology
Root system of plant is root exudates to the various materials of external environment secretion in the vital movement process, root exudates is to surrounding plants (comprising individuality of the same race) and microbial growth is grown the generation promotion or inhibiting phenomenon is called allelopathy.The allelopathy of plant is the important component part of interspecies relation research, it has important directive significance for the development of continuous cropping obstacle, biopesticide and the biological weed killer of research structure of community, the succession of community, biotic intrusion, crop etc., and production has huge using value for agriculture and forestry.
To the research of plant root exudate allelopathy, often adopted and collected the root exudates of plant earlier, and then studied the method for its allelopathy by experiment in the past.Because the common content of root exudates that will collect is very low,, just can carry out STUDY ON ALLELOPATHIC EFFECTS, whole process workload and consuming time all very big after collecting sample so need a large amount of plants and prolongation acquisition time to obtain the secretion of q.s.And traditional root exudates collection technique collects for being placed directly in to collect in the liquid after root system of plant is cleaned, and is bigger with the environmental difference of root growth under the natural conditions, will certainly have influence on the accuracy and the accuracy of experiment; If directly in soil, collect, because soil is a very complicated heterogeneous system, complicated component, spatial and temporal variation is very big, therefore is difficult to collect real, representative secretions from plant roots, also allelopathy that just can not the excellent research sample.
Summary of the invention
In order to overcome problems such as the length consuming time, the workload that exist in the above-mentioned root exudates STUDY ON ALLELOPATHIC EFFECTS are big, precision is low, the utility model proposes a kind of device that when collecting secretions from plant roots, carries out STUDY ON ALLELOPATHIC EFFECTS.
Technical solutions of the utility model are as follows:
Device for researching plant root exudate allelopathy of the present utility model comprises donor plant culturing pot, recipient plant culturing pot, communicating pipe and valve; The bottom of described donor plant culturing pot by one have a valve be communicated to the suitable for reading of recipient plant culturing pot communicating pipe, the bottom of described recipient plant culturing pot is communicated to the suitable for reading of donor plant culturing pot communicating pipe by what be provided with inflation inlet in another pipeline.
The bottom opening of described donor plant culturing pot, opening part seals with rubber stopper, has through hole to be used to insert the communicating pipe that has valve on the rubber stopper.
Described recipient plant culturing pot bottom opening, opening part seals with rubber stopper, has through hole to be used for inserting the communicating pipe that pipeline is provided with inflation inlet on the rubber stopper.
The preferred polyvinyl chloride of material (PVC) material of described donor plant culturing pot and recipient plant culturing pot, thickness is 1.5-2.5mm.Preferred especially dark lighttight PVC material, the perhaps more weak PVC material of light transmission.
Described donor plant culturing pot diameter is 20-30cm, highly is 20-30cm.
Described recipient plant culturing pot diameter is 15-25cm, highly is 15-25cm.
Described communicating pipe is adopted glass material, and communicating pipe wall thickness 1-2mm, internal diameter are 0.8-1.2cm.
Described valve adopts glass material.
Device for researching plant root exudate allelopathy described in the utility model also includes culture matrix quartz sand, and quartz sand places in donor plant culturing pot and the recipient plant culturing pot.Preferably, quartz sand bed thickness 12-28cm.
Device for researching plant root exudate allelopathy described in the utility model is communicated with mouth of pipe place in the bottom of donor plant culturing pot and recipient plant culturing pot and lays the mineral wool layer, leaks down to prevent quartz sand.
Device for researching plant root exudate allelopathy described in the utility model also includes vermiculite, and vermiculite covers on the interior quartz sand of recipient plant culturing pot, to play the effect of preserving moisture.Preferably, vermiculite bed thickness 1-2cm.
The using method of device for researching plant root exudate allelopathy of the present utility model is as follows:
Step 1, when the valve closing state, in the donor plant culturing pot, add quartz sand and on quartz sand plantation donor plant, will the 1-2L nutrient solution add in the donor plant culturing pot and cultivated 2-4 days.
Step 2, in the recipient plant culturing pot, add quartz sand, broadcast seed, cover to play the effect of preserving moisture with the thick vermiculite of 1-2cm on quartz sand top into recipient plant; Then,
Step 3, open valve, the mixing material that contains the donor secretions from plant roots in the donor plant culturing pot was flow in the recipient plant culturing pot through the communicating pipe of bottom.
Liquid in step 4, the recipient plant culturing pot enters into the communicating pipe of its bottom, charge into air by the inflation inlet in this pipeline communicating pipe, liquid in this communicating pipe is back under the effect of air-flow in the donor plant culturing pot, forms the circulation of liquid stream, liquid flowing speed remains on 1Lh-1.
The nutrient solution of step 1 can be selected this area conventional products for use, preferred conventional dicotyledon nutrient solution, and it is as follows to fill a prescription:
K 2SO 4：0.75×10 -3mol·L -1，MgSO 4：0.65×10 -3mol·L -1，KCl：0.1×10 -3mol·L -1，Ca(NO 3) 2：2.0×10 -3mol·L -1，KH 2PO 4：0.25×10 -3mol·L -1，H 3BO 3：1.0×10 -5mol·L -1，MnSO 4：1.0×10 -6mol·L -1，CuSO 4：1.0×10 -7mol·L -1，ZnSO 4：1.0×10 -6mol·L -1，(NH 4) 6Mo 7O 24：1.0×10 -8mol·L -1，Fe-EDTA：1.0×10 -4mol·L -1。
Situation about consuming according to nutrient solution in whole experiment is replenished nutrient solution in right amount, and this is well-known to those skilled in the art, does not do special qualification at this.
After emerging, recipient plant measures emergence rate, measure plant height, leaf area, biomass, photosynthetic rate, root system vigor, SOD, POD, CAT, MDA etc. when seedling stage, florescence, measure STUDY ON ALLELOPATHIC EFFECTS indexs such as biomass, output during the maturing stage, (use with contrast with the same method of sampling device, but the donor plant culturing pot is not planted plant) contrast after, can draw the allelopathy of donor secretions from plant roots to recipient plant.
The beneficial effects of the utility model are: owing to adopted secretions from plant roots is collected the method that the process with STUDY ON ALLELOPATHIC EFFECTS combines together, and with quartz sand as cultivation medium, the natural environment of simulating plant growth better, disturbing factor is few in the research process, thereby has improved operating efficiency, has reduced workload, improved accuracy and the accuracy of research.The amount of donor plant and recipient plant cultivation, developmental stage etc. can be regulated and control, and make up at random, thereby more help the theoretical research of plant root exudate allelopathy.Use device of the present utility model with adopt conventional method mutually specific efficiency improve 1-3 doubly, workload reduces more than 1 half, precision improves more than 20%.
Description of drawings
Fig. 1 is the structural representation of device for researching plant root exudate allelopathy.Wherein, 1 donor plant culturing pot, 2 culture matrix quartz sands, 3 mineral wool layers, 3 ' mineral wool layer, 4 rubber stoppers, 5 communicating pipes, 6 valves, 7 recipient plant culturing pots, 8 vermiculites, 9 rubber stoppers, 10 communicating pipes, 11 inflation inlets.Arrow representative in Fig. 1 pipeline contains the flow direction of the mixing material of donor secretions from plant roots.
Embodiment
Below in conjunction with embodiment the utility model is described further.The device feature material that uses among the embodiment is as follows:
Donor plant culturing pot, recipient plant culturing pot are the PVC material,
Communicating pipe and valve all adopt glass material, and communicating pipe wall thickness 1-2mm, internal diameter are 0.8-1.2cm.
Embodiment 1: device for researching plant root exudate allelopathy, structure are as shown in Figure 1.The bottom of donor plant culturing pot 1 has the rubber stopper 4 of opening to link to each other with communicating pipe 5 by the center, the end of communicating pipe 5 is communicated with the bottom of donor plant culturing pot 1, and the other end passes to the suitable for reading of recipient plant culturing pot 7, and valve 6 was located on communicating pipe 5; The bottom opening of recipient plant culturing pot 7 linked to each other with communicating pipe 10, and the other end of communicating pipe 10 passes to the suitable for reading of donor plant culturing pot 1, was provided with inflation inlet 11 in communicating pipes 10 pipelines.
Donor plant culturing pot 1 diameter is 25cm, highly is 30cm.Be communicated with mouth of pipe place in the bottom of donor plant culturing pot 1 and be covered with mineral wool layer 3, be covered with culture matrix quartz sand 2 on it, quartz sand bed thickness 25cm.
Recipient plant culturing pot 7 diameters are 20cm, highly are 20cm.Be communicated with in the bottom of recipient plant culturing pot 7 mouth of pipe place lay mineral wool layer 3 ', be covered with culture matrix quartz sand 2 on it, quartz sand bed thickness 15cm.Be coated with the thick vermiculite 8 of 1.5cm on the quartz sand.
Embodiment 2: structure such as embodiment 1, the bottom of different is recipient plant culturing pot 7 has the rubber stopper 9 of opening to link to each other with communicating pipe 10 by the center.
The utility model has carried out the actual detected application in research work, obtained experiment effect preferably, the utility model with adopt conventional method mutually specific efficiency improve 2 times, workload reduces 1 half, precision improves 30%.

Claims (10)

1. a device for researching plant root exudate allelopathy is characterized in that comprising donor plant culturing pot, recipient plant culturing pot, communicating pipe and valve; The bottom of described donor plant culturing pot by one have a valve be communicated to the suitable for reading of recipient plant culturing pot communicating pipe, the bottom of described recipient plant culturing pot is communicated to the suitable for reading of donor plant culturing pot communicating pipe by what be provided with inflation inlet in another pipeline.
2. device for researching plant root exudate allelopathy as claimed in claim 1 is characterized in that, the bottom opening of described donor plant culturing pot, and opening part seals with rubber stopper, has through hole to be used to insert the communicating pipe that has valve on the rubber stopper.
3. device for researching plant root exudate allelopathy as claimed in claim 1 is characterized in that, described recipient plant culturing pot bottom opening, and opening part seals with rubber stopper, has through hole to be used for inserting the communicating pipe that pipeline is provided with inflation inlet on the rubber stopper.
4. device for researching plant root exudate allelopathy as claimed in claim 1 is characterized in that, also includes culture matrix quartz sand, and quartz sand places in donor plant culturing pot and the recipient plant culturing pot.
5. device for researching plant root exudate allelopathy as claimed in claim 4 is characterized in that, is communicated with mouth of pipe place in the bottom of donor plant culturing pot and recipient plant culturing pot and is covered with the mineral wool layer.
6. device for researching plant root exudate allelopathy as claimed in claim 4 is characterized in that, has vermiculite to cover on the interior quartz sand of recipient plant culturing pot.
7. device for researching plant root exudate allelopathy as claimed in claim 1 is characterized in that, the material of described donor plant culturing pot and recipient plant culturing pot is polyvinyl chloride (PVC) material.
8. as claim 1 or 7 described device for researching plant root exudate allelopathy, it is characterized in that described donor plant culturing pot diameter is 20-30cm, highly is 20-30cm.
9. as claim 1 or 7 described device for researching plant root exudate allelopathy, it is characterized in that described recipient plant culturing pot diameter is 15-25cm, highly is 15-25cm.
10. device for researching plant root exudate allelopathy as claimed in claim 1 is characterized in that, described communicating pipe is adopted glass material, and communicating pipe wall thickness 1-2mm, internal diameter are 0.8-1.2cm.
CN2009202906000U 2009-12-25 2009-12-25 Device for researching plant root exudate allelopathy Expired - Fee Related CN201624028U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101762687B (en) * 2010-01-12 2013-03-20 山东省农业科学院土壤肥料研究所 Analysis test method of allelopathy of plant organ water-soluble extract
CN111624313A (en) * 2020-06-23 2020-09-04 东北林业大学 Research device and method for allelopathy of volatile organic compounds of plant root system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101762687B (en) * 2010-01-12 2013-03-20 山东省农业科学院土壤肥料研究所 Analysis test method of allelopathy of plant organ water-soluble extract
CN111624313A (en) * 2020-06-23 2020-09-04 东北林业大学 Research device and method for allelopathy of volatile organic compounds of plant root system

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Granted publication date: 20101110

Termination date: 20111225