CN209931249U

CN209931249U - Simple zebra fish spawning control and quick spawn taking device for laboratory

Info

Publication number: CN209931249U
Application number: CN201920630277.0U
Authority: CN
Inventors: 祝凌燕; 杨荣艳; 钟文珏; 杨艺; 陈新
Original assignee: Nankai University
Current assignee: Nankai University
Priority date: 2019-05-06
Filing date: 2019-05-06
Publication date: 2020-01-14
Anticipated expiration: 2029-05-06

Abstract

Simple zebra fish spawning control and quick spawn taking device is used in laboratory. The method is mainly used for egg laying control and rapid collection of eggs of a large number of zebra fishes in a laboratory or a family. The device consists of a light-tight glass cylinder body, a cover plate with a natural light source lamp tube, a thermometer, a transparent pluggable glass baffle, a mesh inner cylinder and a collecting device. The design can stimulate the zebra fish to lay eggs and obtain the eggs with the same hpf (waters post fertilization) by controlling the illumination temperature, and the fish eggs enter the collecting device through the sieve pores on the bottom surface of the mesh-shaped inner vat, so that the zebra fish is prevented from eating the fish eggs, the collecting device is removed, the collecting net at the bottom of the collecting device is separated, and the zebra fish is cleaned and undergoes the fertilization rate statistics, incubation or other follow-up operations. The biggest advantage of this device is in that the whole quick not damaged of zebra fish egg is collected.

Description

Simple zebra fish spawning control and quick spawn taking device for laboratory

Technical Field

The utility model discloses the device mainly is applied to the complete quick collection of spawning control and roe of the great amount of zebra fish in laboratory.

Background

Zebra fish is a small tropical fish, and its gene has high homology of 87% with human gene, and is easy to raise and reproduce, low in cost and easy to distinguish male and female. Therefore, besides ornamental value, zebra fish is used as a model organism under laboratory conditions, and is commonly used for water quality environment monitoring and research on toxicity, action mechanism and the like of some environmental poisons.

The zebra fish is fertilized in vitro, the main factors influencing the spawning of the zebra fish are illumination and temperature, and the zebra fish with the proper age can spawn all the year round under the proper illumination and temperature.

After the zebra fish spawns, the zebra fish has a habit of eating eggs, so that the survival rate of the zebra fish eggs is low.

In laboratory research, the influence of certain factors on the egg laying amount, the fertilization rate, the hatching rate, the survival rate and the offspring of the zebra fish is often required to be tested, or the influence of certain factors on the alternate generation of the zebra fish is required to be researched. The currently used method for collecting the eggs in a relatively large number is a siphon method, and the operation is time-consuming and relatively complicated.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problem of collecting the eggs of the zebra fish after spawning; firstly, the zebra fish is controlled to mate and lay eggs by artificially controlling the temperature and illuminating the partition plate, and secondly, the moving area of the zebra fish is limited by the design of the net-shaped inner tank of the zebra fish, so that the zebra fish is prevented from eating eggs. Finally, the combination of the collecting net and the inner tank enables the roe to be completely collected and quickly taken out of the tank, which is the most advantage of the device.

The technical scheme of the utility model

The simple zebra fish spawning control and rapid spawn taking device for the laboratory mainly comprises an opaque glass outer cylinder, a cover plate with a natural light source lamp tube and a thermometer, wherein a net-shaped inner cylinder is arranged in the glass outer cylinder through a sucking disc, a transparent glass baffle is inserted into the net-shaped inner cylinder, a collecting device is arranged below the net-shaped inner cylinder, and the inner cylinder and the outer cylinder are in a completely communicated state; wherein, the reticular inner tank is slightly lower than the glass outer tank, the distance from the water level for fish culture to the upper port of the inner tank is more than 5cm (zebra fish easily jump out of the inner tank when the water level is too high). The inner walls of two opposite side surfaces of the reticular inner cylinder are respectively provided with a groove so as to be convenient for inserting and fixing the transparent glass baffle.

The outer glass jar is made of opaque glass, so that the light and dark conditions of 14:10 can be controlled manually through a natural light source lamp tube on the cover plate, and zebra fish can be stimulated to lay eggs.

Wherein the aperture (2-3mm) of five face nets of netted inner vat is less than zebra fish cross section diameter to the aperture of bottom surface net is greater than the diameter of zebra fish ovum, collects the diameter of net and is less than zebra fish ovum diameter (but avoid too closely be convenient for fish out), can prevent like this that the zebra fish from swimming out the intranet, can guarantee again that all roes are smooth to pass through the sieve mesh of netted inner vat ground bottom surface and all fall into collection device.

Wherein, the netted inner tank is fixed on the inner surface of the glass outer tank through 6 (can increase and decrease by oneself according to design shape and size) plastic suckers, so that the netted inner tank is fixed by fully utilizing the advantages of the glass surface, and the detachment and the taking of the netted inner tank are facilitated.

Wherein collection device comprises rigid plastic curb plate all around, and the inboard of curb plate is seted up flutedly, and the bottom is the collection net of compriseing rubber strip frame all around and middle nylon cloth, and detachable nylon cloth inlays the dress in the recess of curb plate through the rubber strip frame, and the height of collection device curb plate is 1.5-3CM, and collection device is whole to be connected with netted interior jar bottom through the detachable buckle or the couple of curb plate top.

Wherein, a thermometer fixing hole is arranged on the reticular inner cylinder, so that a thermometer can be used for observing the real-time water temperature conveniently.

The utility model has the advantages and beneficial effects as follows:

1. the device can artificially control the illumination temperature required by the spawning of the zebra fish and promote the zebra fish in the sexual maturity stage to spawn. And zebra fish eggs in the same period are obtained through the control of the clapboard.

2. The zebra fish roe enters the collecting net through the sieve pores on the bottom surface of the mesh-shaped inner tank, so that the zebra fish is prevented from eating the roe, and the survival rate of the zebra fish roe is improved.

3. Netted inner cylinder and collection device can be simultaneously conveniently taken out from the jar to inner cylinder and collection device and collection net are separable, and the whole quick not damaged of zebra fish egg of being convenient for is collected and is counted, and is safe convenient high-efficient more than traditional siphon method, and this is the biggest bright spot of this device also.

Drawings

FIG. 1 is a simple diagram of the simple zebra fish spawning control and rapid spawn taking device for the laboratory;

fig. 2 is the utility model discloses the control of laying eggs with simple and easy zebra fish in laboratory and get the ovum device and explain the schematic diagram in detail fast.

In the figure, 1 cover plate, 2 lamp tube, 3 thermometer, 4 glass outer cylinder, 5 sucker, 6 netted inner cylinder (bottom with sieve plate), 7 collecting net plastic side plate, 8 buckle, 9 adhesive tape, 10 nylon net, 11 adhesive tape cross section, 12 collecting net plastic side plate cross section, 13 glass baffle.

Detailed Description

In order to make the technical problems, technical solutions and advantages of the present invention to be solved clearer, the following detailed description will be made with reference to the accompanying drawings, specific implementation processes and application ranges.

The utility model device will be described first with reference to the accompanying drawings. As shown in fig. 1 and 2, the simple zebra fish spawning control and rapid spawning device for the laboratory mainly comprises an opaque glass outer jar 4, a cover plate 1 with a natural light source lamp tube 2 and a thermometer 3, wherein a net-shaped inner jar 6 is arranged in the glass outer jar through a sucker 5, a transparent glass baffle 13 is inserted in the net-shaped inner jar, a collecting device is arranged below the net-shaped inner jar, and the inner jar and the outer jar are in a completely communicated state; wherein, the reticular inner tank is slightly lower than the glass outer tank, the distance from the water level for fish culture to the upper port of the inner tank is more than 5cm (zebra fish easily jump out of the inner tank when the water level is too high). The inner walls of two opposite side surfaces of the reticular inner cylinder are respectively provided with a groove so as to be convenient for inserting and fixing the transparent glass baffle. The collecting device is composed of peripheral rigid plastic side plates 7, grooves are formed in the inner sides of the side plates, the bottom of the collecting device is a collecting net composed of a frame formed by peripheral rubber strips 9 and a nylon net 10 in the middle, and the detachable nylon cloth is embedded in the grooves of the side plates through the rubber strips. The height of the side plate of the collecting device is 1.5-3CM, and the whole collecting device is connected with the bottom of the reticular inner cylinder through a detachable buckle 8 above the side plate.

The light-tight design of the outer jar is combined with the lamp tube of the cover plate, so that the illumination condition required by the spawning of the zebra fish can be controlled manually. The mesh diameter of five faces of inner vat all is less than zebra fish body cross section diameter to sieve mesh diameter on the bottom surface is greater than roe diameter, so that zebra fish roe is whole smoothly through the sieve mesh and all collected the net and collect, and the net design not only prevents that zebra fish from swimming out the intranet, and is convenient for the exchange of inside and outside water body. The design of the removable partition plate of the inner tank can control the mating time of zebra fish so as to obtain fish eggs in the same period, and the removable partition plate is mainly used for the aspect of laboratory research. The thermometer is convenient for monitoring the water temperature in real time so as to control the water temperature to be within the proper living temperature and spawning temperature range of the zebra fish.

Taking the example of the zebra fish spawning, when in use, the bottom of the net-shaped inner jar is provided with a clean collecting net which is arranged in a matched glass jar, and the sucking disc is fixed on the inner surface of the outer jar. Water is injected into the jar to a position 5cm away from the upper edge of the inner jar (which can be properly adjusted according to specific conditions), a glass partition plate is inserted into a slot of the inner jar (aiming at controlling to obtain zebra fish eggs in the same period, the zebra fish eggs can not be placed), a thermometer is placed to control the water temperature to be about 24 ℃ through a temperature control device, a proper amount of male and female zebra fish are respectively placed on the left side and the right side of the inner jar, a cover plate is added, and the illumination is manually controlled, so that the illumination-dark time ratio is controlled to be about 14: 10. The next morning, the septum was removed, the tube opened, and the eggs were removed two hours later. The specific operation is as follows: loosen the sucking disc of outer jar inner wall and fish out the inner tank, transfer the fish among them to other jars and raise, loosen the buckle and take off from the inner tank bottom and collect the net to carefully take off the screen cloth, wash and screen the inspection so that follow-up use the zebra fish egg on the screen cloth.

The device has a simple structure but rich functions, and can be applied to the following situations.

1. A large batch of contemporary zebra fish eggs are obtained in a laboratory, collected and used for subsequent hatching.

2. The laboratory researches the egg laying amount, fertility rate, hatching rate, survival rate and the like of the zebra fish.

3. Collecting and hatching the zebra fish eggs for home viewing.

4. Oviposition of other oviparous fish and collection of fish eggs.

The collection net in this device can collect excrement and urine and fish out when collecting the roe to purify quality of water, avoided having practiced thrift the water source simultaneously because of the influence of the stable environment of surviving of zebra fish of water change on duty.

Claims

1. The utility model provides a laboratory is with simple and easy zebra fish control of laying eggs and get ovum device fast, the device include the glass outer jar, have apron and thermometer of natural light source fluorescent tube, its characterized in that, the glass outer jar be opaque glass outer jar, be equipped with netted inner cylinder through the sucking disc in the glass outer jar, can insert transparent glass baffle in the netted inner cylinder, the below of netted inner cylinder is provided with collection device, inner and outer jar is in complete communicating state.

2. The laboratory is with simple and easy zebra fish spawning control and quick ovum pick-up device of claim 1, characterized in that, the aperture of five face nets of netted inner jar is less than zebra fish cross section diameter, and the aperture of bottom net is greater than the diameter of zebra fish ovum, so can prevent zebra fish from wandering out the intranet, can guarantee that the fish ovum is all smoothly collected by the collection device below netted inner jar.

3. The laboratory is with simple and easy zebra fish spawning control and quick ovum device of getting it to claim 1, characterized by, collection device comprises rigid plastic curb plate all around, and the inboard of curb plate is seted up flutedly, and the bottom is the collection net that comprises rubber strip frame all around and middle nylon cloth, collects the aperture of net and will be less than the diameter of roe, collects in the recess of rubber strip frame embedding curb plate in the net.

4. The laboratory is with simple and easy zebra fish spawning control and quick ovum retrieval device of claim 3, characterized in that, collect the net and collect the net for nylon material to prevent that too hard material from damaging the zebra fish ovum.

5. The laboratory simple zebra fish spawning control and rapid spawn taking device as claimed in claim 1, wherein the side length of the bottom surface of the mesh-shaped inner cylinder is slightly smaller than the side length of the inner side of the glass outer cylinder, so that the mesh-shaped inner cylinder can be conveniently inserted and taken out, and a movement space as large as possible can be provided for the zebra fish in the cylinder.

6. The device for controlling spawning and quickly taking eggs of the simple zebra fish for the laboratory according to claim 3, wherein the collecting device is connected with the bottom of the mesh-shaped inner cylinder through a buckle or a hook, and the collecting device can be freely disassembled and assembled as required, so that the eggs can be conveniently taken.

7. The laboratory simple zebra fish spawning control and quick spawning device as claimed in claim 6, wherein the collecting device is fixed at the bottom of the mesh-shaped inner tank through a buckle above the side plate, and is safe, firm, and convenient to disassemble, so as to facilitate replacement and cleaning and collection of spawns.

8. The laboratory simple zebra fish spawning control and rapid spawn taking device as claimed in claim 1, wherein a groove is formed on each of two opposite side inner walls of the meshed inner cylinder, and a transparent glass baffle is inserted into the meshed inner cylinder through the grooves so that male and female zebra fish can see each other, the zebra fish is stimulated to spawn, and zebra fish spawns in the same period are obtained by being controlled by inserting and pulling the transparent glass baffle.