CN209759474U - Oat polypeptide extraction and purification system - Google Patents
Oat polypeptide extraction and purification system Download PDFInfo
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- CN209759474U CN209759474U CN201822157841.XU CN201822157841U CN209759474U CN 209759474 U CN209759474 U CN 209759474U CN 201822157841 U CN201822157841 U CN 201822157841U CN 209759474 U CN209759474 U CN 209759474U
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- oat
- feed inlet
- ultrafiltration
- oat polypeptide
- enzymolysis
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Abstract
The utility model discloses an oat polypeptide draws purification system, include: pretreatment system, ultrafiltration equipment, enzymolysis tank, nanofiltration system, vacuum drying equipment. The pretreatment system is used for pretreating oat. The feed inlet of the ultrafiltration device is connected with the discharge outlet of the pretreatment system and is used for ultrafiltering monosaccharide, polysaccharide, dietary fiber, inorganic salt and other components in the pretreated oat. The feed inlet of the enzymolysis tank is connected with the discharge outlet of the ultrafiltration equipment and is used for carrying out enzymolysis on the oat subjected to ultrafiltration to obtain the oat protein aqueous solution. And a feed inlet of the nanofiltration system is connected with a discharge outlet of the enzymolysis tank and is used for removing undecomposed protein, residual enzyme and the like to obtain oat polypeptide liquid after enrichment and concentration. The feed inlet of the vacuum drying equipment is connected with the discharge outlet of the nanofiltration system and is used for vacuum drying the oat polypeptide liquid into oat polypeptide powder. By adopting an enzymolysis method and a nanofiltration system to carry out enrichment and concentration on the oat polypeptide, the extraction purity of the oat polypeptide is improved.
Description
Technical Field
The utility model belongs to the technical field of bioactive composition, especially, relate to an oat polypeptide draws purification system.
background
The oat is rich in nutrition, has higher contents of protein, fat, vitamin E and mineral elements than other cereal crops, and has medical value and health care effects of lowering blood pressure, lowering cholesterol, preventing and treating colorectal cancer, preventing and treating heart diseases and the like. The oat protein has the amino acid balance which is the best in the grains, the essential amino acid composition is relatively balanced, the content of various essential amino acids is higher than or close to the FAO/WHO recommended amino acid standard pattern value, and particularly, the content of lysine is higher than 1 time of wheat flour.
the peptides are the constituent parts of proteins and are formed by mutually connecting amino acids through amido bonds, a large number of researches show that the bioactive polypeptides have various physiological functions beneficial to human bodies, such as functions of resisting oxidation, resisting tumors, regulating immunity, inhibiting bacteria, reducing blood pressure, reducing blood sugar and the like, are easy to digest, absorb and utilize by the human bodies, have good stability, good physical processing characteristics and a plurality of functional characteristics, and the oat polypeptides have become a hotspot in current food science researches.
In the pharmaceutical industry, peptides and polypeptides are usually purified from organic or aqueous solutions by column or chromatography and further concentrated by heat evaporation under vacuum. Since the peptide concentration is too low, only 0.1% to 0.5%, the evaporation process is too long in duration, possibly destroying the purified product, while also consuming large amounts of organic/aqueous leachates.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide an oat polypeptide draws purification system, takes enzymolysis method and nanofiltration system to carry out the enrichment concentration to oat polypeptide, has improved oat polypeptide's extraction purity.
in order to solve the above problem, the technical scheme of the utility model is that:
The utility model discloses an oat polypeptide draws purification system, include:
The pretreatment system is used for pretreating the oats;
The feed inlet of the ultrafiltration equipment is connected with the discharge outlet of the pretreatment system and is used for ultrafiltering the pretreated components of monosaccharide, polysaccharide, dietary fiber, inorganic salt and the like in the oat;
the feed inlet of the enzymolysis tank is connected with the discharge outlet of the ultrafiltration equipment, and the oat subjected to ultrafiltration is used for enzymolysis to obtain an oat protein aqueous solution;
A feed inlet of the nanofiltration system is connected with a discharge outlet of the enzymolysis tank and is used for removing undecomposed protein, residual enzyme and the like in the oat protein aqueous solution to obtain enriched and concentrated oat polypeptide liquid;
The feed inlet of the vacuum drying equipment is connected with the discharge outlet of the nanofiltration system and is used for vacuum drying the oat polypeptide liquid into oat polypeptide powder;
the pretreatment system comprises a peeling machine, a pulverizer and a colloid mill; wherein the discharge hole of the peeling machine is connected with the feed inlet of the pulverizer; the discharge hole of the pulverizer is connected with the feed inlet of the colloid mill, and the discharge hole of the colloid mill is connected with the feed inlet of the ultrafiltration device; the colloid mill is provided with a water inlet.
The utility model discloses an oat polypeptide draws purification system the discharge gate of colloid mill with be equipped with first force (forcing) pump between ultrafiltration apparatus's the feed inlet.
The utility model discloses an oat polypeptide draws purification system, one side of ultrafiltration apparatus still is connected with back flush equipment.
the utility model discloses an oat polypeptide draws purification system, one side of enzymolysis tank is connected with the batching jar.
The utility model discloses an oat polypeptide draws purification system, the discharge gate of enzymolysis tank with be equipped with the second force (forcing) pump between the feed inlet of nanofiltration system.
The utility model discloses an oat polypeptide extraction and purification system, the ultrafiltration device is provided with a first slag discharge port; the nanofiltration system is provided with a second slag discharge port.
The utility model discloses owing to adopt above technical scheme, make it compare with prior art and have following advantage and positive effect:
1) In one embodiment of the utility model, the oat polypeptide is enriched and concentrated by adopting an enzymolysis method and a nanofiltration system, so that the extraction purity of the oat polypeptide is improved;
2) In one embodiment of the utility model, the ultrafiltration device is arranged in front of the enzymolysis tank, which can crudely extract the materials and discharge monosaccharide, polysaccharide, dietary fiber, inorganic salt and other components, thus improving the extraction efficiency of oat polypeptide;
3) The utility model discloses an embodiment, to the oat through preliminary treatment and ultrafiltration take enzymolysis to extract and exempt from the equipment loss that conventional alkaline process extracted the existence fast, the polypeptide activity is low, waste liquid pollution environment scheduling problem.
Drawings
Fig. 1 is a schematic diagram of the oat polypeptide extraction and purification system of the present invention.
Description of reference numerals:
1: a peeling machine; 2: a pulverizer; 3: colloid milling; 4: a water inlet; 5: a first pressure pump; 6: ultrafiltration equipment; 7: a backwashing device; 8: a first slag discharge port; 9: an enzymolysis tank; 10: a dosing tank; 11: a second pressure pump; 12: a nanofiltration system; 13: a second slag discharge port; 14: and (5) vacuum drying equipment.
Detailed Description
The following provides a further detailed description of the oat polypeptide extraction and purification system provided by the present invention with reference to the accompanying drawings and specific examples. The advantages and features of the present invention will become more fully apparent from the following description and appended claims.
Referring to fig. 1, in one embodiment, an oat polypeptide extraction and purification system comprises: a pretreatment system, an ultrafiltration device 6, an enzymolysis tank 9, a nanofiltration system 12 and a vacuum drying device 14. The pretreatment system is used for pretreating oat. The feed inlet of the ultrafiltration device 6 is connected with the discharge outlet of the pretreatment system and is used for ultrafiltering monosaccharide, polysaccharide, dietary fiber, inorganic salt and other components in the pretreated oat. The feed inlet of the enzymolysis tank 9 is connected with the discharge outlet of the ultrafiltration device 6, the oat subjected to ultrafiltration is used for enzymolysis to obtain an oat protein aqueous solution, the temperature in the whole enzymolysis process is controlled to be 40-50 ℃, the enzymolysis time is 3-5h, and the enzyme adding amount is 3-5%. A feed inlet of the nanofiltration system 12 is connected with a discharge outlet of the enzymolysis tank 9 and is used for removing undecomposed protein, residual enzyme and the like in the oat protein water solution to obtain enriched and concentrated oat polypeptide liquid. The feed inlet of the vacuum drying device 14 is connected with the discharge outlet of the nanofiltration system 12 and is used for vacuum drying the oat polypeptide liquid into oat polypeptide powder. By adopting an enzymolysis method and a nanofiltration system to carry out enrichment and concentration on the oat polypeptide, the extraction purity of the oat polypeptide is improved. By arranging the ultrafiltration equipment in front of the enzymolysis tank, the material can be crudely extracted, monosaccharide, polysaccharide, dietary fiber, inorganic salt and other components are discharged, and the extraction efficiency of oat polypeptide is improved. The problems of quick equipment loss, low polypeptide activity, environmental pollution caused by waste liquid and the like in the conventional alkaline extraction are solved by extracting the oat subjected to pretreatment and ultrafiltration by an enzymolysis method.
Furthermore, the aperture size of an ultrafiltration membrane of the ultrafiltration equipment is 200KDa-500KDa, and the aperture size of a nanofiltration membrane of the nanofiltration system is 100Da-200 Da.
Further, the pretreatment system comprises a peeling machine 1, a pulverizer 2 and a colloid mill 3, wherein a discharge hole of the peeling machine 1 is connected with a feed inlet of the pulverizer 2; the discharge hole of the pulverizer 2 is connected with the feed inlet of the colloid mill 3, and the discharge hole of the colloid mill 3 is connected with the feed inlet of the ultrafiltration device 6. The colloid mill 3 is provided with a water inlet 4. Oat is fed, peeled by a peeling machine 1 and conveyed to a crusher 2 for primary crushing, the oat is conveyed to a colloid mill 3 for fine grinding after primary crushing, deionized water is fed into the colloid mill through a water inlet 4, the ratio of the material to the deionized water is 1:10-1:20, and the granularity of ground pulp is controlled below 50 nanometers.
Further, a first pressure pump 5 is arranged between the discharge hole of the colloid mill 3 and the feed hole of the ultrafiltration device 6. The material in conveying is pressurized by the first pressurizing pump 5, the ultrafiltration pressure of the ultrafiltration equipment 6 is controlled to be 0.2MPa-0.5MPa, and the extraction efficiency of the oat polypeptide is further improved.
preferably, a back washing device 7 is connected to one side of the ultrafiltration device 6, so that the ultrafiltration device 6 can be cleaned after the ultrafiltration process is finished.
Preferably, a dosing tank 10 is connected to one side of the enzymatic hydrolysis tank 9. The batching tank 10 is used for preparing compound protease required by enzymolysis.
Further, a second pressure pump 11 is arranged between the discharge port of the enzymolysis tank 9 and the feed port of the nanofiltration system 12. The pressure of the oat protein hydrolysate is adjusted to 0.1Mpa by the second pressure pump 11, the nanofiltration operation time is 1-2h, and the extraction efficiency of oat polypeptide is further improved.
Further, the ultrafiltration device 6 is provided with a first residue outlet 8 for discharging monosaccharide, polysaccharide, dietary fiber, inorganic salt and other components. The nanofiltration system 12 is provided with a second reject port 13 to discharge undecomposed proteins, remaining enzymes, etc., while discharging low molecular salts, etc.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, the changes are still within the scope of the present invention if they fall within the scope of the claims and their equivalents.
Claims (6)
1. An oat polypeptide extraction and purification system, comprising:
the pretreatment system is used for pretreating the oats;
The feed inlet of the ultrafiltration equipment is connected with the discharge outlet of the pretreatment system and is used for ultrafiltering monosaccharide, polysaccharide, dietary fiber and inorganic salt in the pretreated oat;
The feed inlet of the enzymolysis tank is connected with the discharge outlet of the ultrafiltration equipment, and the oat subjected to ultrafiltration is used for enzymolysis to obtain an oat protein aqueous solution;
A feed inlet of the nanofiltration system is connected with a discharge outlet of the enzymolysis tank and is used for removing undecomposed protein and residual enzyme in the oat protein aqueous solution to obtain enriched and concentrated oat polypeptide liquid;
the feed inlet of the vacuum drying equipment is connected with the discharge outlet of the nanofiltration system and is used for vacuum drying the oat polypeptide liquid into oat polypeptide powder;
the pretreatment system comprises a peeling machine, a pulverizer and a colloid mill; wherein the discharge hole of the peeling machine is connected with the feed inlet of the pulverizer; the discharge hole of the pulverizer is connected with the feed inlet of the colloid mill, and the discharge hole of the colloid mill is connected with the feed inlet of the ultrafiltration device; the colloid mill is provided with a water inlet.
2. the system for extracting and purifying oat polypeptide according to claim 1, characterized in that a first pressure pump is arranged between the discharge port of the colloid mill and the feed port of the ultrafiltration device.
3. the system for extracting and purifying oat polypeptide according to claim 1, characterized in that a back washing device is connected to one side of the ultrafiltration device.
4. The oat polypeptide extraction and purification system of claim 1, wherein a dosing tank is connected to one side of the enzymolysis tank.
5. the system for extracting and purifying oat polypeptide according to claim 1, characterized in that a second pressure pump is arranged between the discharge port of the enzymolysis tank and the feed port of the nanofiltration system.
6. the system for extracting and purifying oat polypeptide according to claim 1, wherein the ultrafiltration device is provided with a first slag discharge port; the nanofiltration system is provided with a second slag discharge port.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822157841.XU CN209759474U (en) | 2018-12-21 | 2018-12-21 | Oat polypeptide extraction and purification system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201822157841.XU CN209759474U (en) | 2018-12-21 | 2018-12-21 | Oat polypeptide extraction and purification system |
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| CN209759474U true CN209759474U (en) | 2019-12-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113443966A (en) * | 2021-06-24 | 2021-09-28 | 德兰梅勒（北京）分离技术股份有限公司 | Extraction device and extraction method of resveratrol |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113443966A (en) * | 2021-06-24 | 2021-09-28 | 德兰梅勒（北京）分离技术股份有限公司 | Extraction device and extraction method of resveratrol |
| CN113443966B (en) * | 2021-06-24 | 2022-10-04 | 德兰梅勒（北京）分离技术股份有限公司 | Extraction device and extraction method of resveratrol |
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