CN209745989U

CN209745989U - Qualitative/quantitative biochip detection device

Info

Publication number: CN209745989U
Application number: CN201822220979.XU
Authority: CN
Inventors: 周君; 苏秀榕; 芦晨阳; 李妍妍
Original assignee: Ningbo University
Current assignee: Zhejiang Zhenghegu Biotechnology Co.,Ltd.
Priority date: 2018-12-27
Filing date: 2018-12-27
Publication date: 2019-12-06
Anticipated expiration: 2028-12-27

Abstract

The utility model discloses a qualitative/quantitative biochip detection device, characteristics are including detecting the box and detecting the box, are provided with image display mechanism on the detection box, are provided with biochip reaction mechanism, light source and image capture mechanism in the detection box, and image capture mechanism is connected with image display mechanism electricity. The advantage is that above-mentioned detection device is integrated in a detection box with biochip's reaction and detection, and the back is accomplished in the reaction, provides the light source for biochip when detecting through the light source, then catches the image information on the biochip and sends the image information who catches to image display mechanism through image capture mechanism, and simple structure, portability are good and can obtain comparatively accurate observation result.

Description

Qualitative/quantitative biochip detection device

Technical Field

the utility model belongs to the technical field of biochip detection and specifically relates to a qualitative/quantitative biochip detection device is related to.

Background

The biochip (biochip or bioarray) integrates biochemical analysis process on the chip surface according to the principle of specific interaction between biological molecules, thereby realizing high-flux rapid detection of DNA, RNA, polypeptide, protein and other biological components. The narrow sense of biochip concept refers to a biomolecule lattice formed by immobilizing biomolecules (oligonucleotides, cDNAs, genomic DNAs, polypeptides, antibodies, antigens, etc.) on a solid phase transmitter such as a silicon wafer, a glass plate (beads), a plastic plate (beads), a gel, a nylon membrane, etc. by various methods. The concept of a chip is taken from the concept of integration, a biochip being the integration of biological material. Like laboratory tests, the examination of blood glucose, proteins, enzyme activities, etc. on biochips is based on the same biological reaction principles. Therefore, the biochip is a carrier platform, is a carrier, and is a micro biochemical analysis system which integrates discontinuous analysis processes in the life science field on the surface of a substrate so as to realize accurate, rapid and large-information-quantity detection on cells, proteins, genes and other biological components. The biochip has the technical advantages that the detection is faster, more sensitive and more accurate.

the conventional biochip detection firstly needs to react with a biological sample, and after the reaction is finished, the specific detection and research aiming at the biochip are mainly carried out by two modes, one mode is that the biochip is observed by naked eyes of a human body by means of a microscope, and then the analysis and the research are carried out according to the observed result; the other method is to scan the biochip with a scanner, input the scanning result into a computer, observe the scanning result with the computer, and conduct in-depth research according to the observation result. The two methods mainly have the following problems: in the former, the biochip is observed by naked eyes of a human body by means of a microscope, but the observation result is difficult to record, and a sketch of the observation result can be drawn only by observing while the observation is carried out, which has high requirements on the drawing capability of an observer, so that the detection and research mode is inconvenient to record the observation result, and the accuracy of the observation result is difficult to ensure, thereby causing certain influence on subsequent research; although the latter can obtain a clear observation result by combining the scanner and the computer, the scanner and the computer must coexist in the detection mode, and the scanner and the computer are not necessary, if only can scan the biochip, the scanning result cannot be displayed, if only can the computer can not scan the biochip, the observation result cannot be obtained, and the scanner and the computer are large in size, poor in portability and inconvenient to use. Meanwhile, when a biological sample is reacted, equipment such as a nucleic acid amplification instrument, a chip hybridization instrument, a chip cleaning machine and the like is required, and the whole set of related equipment in the current market is expensive, large and heavy in size, not suitable for frequent movement and difficult to move.

Disclosure of Invention

The utility model aims to solve the technical problem that a qualitative/quantitative biochip detecting system that is small, is convenient for remove and can obtain comparatively accurate observation result is provided.

The utility model provides a technical scheme that above-mentioned technical problem adopted does:

The qualitative/quantitative biochip detecting device includes one detecting box and one detecting box, the detecting box has one image display mechanism, one biochip reaction mechanism, one light source and one image capturing mechanism set inside the detecting box, and the image capturing mechanism is electrically connected to the image display mechanism.

The detection box body is characterized in that the front end face of the detection box body is provided with the image display mechanism, the image display mechanism is a display screen, the rear end of the detection box body is provided with a plurality of detection cavities matched with the detection box, the detection box is detachably inserted and arranged in the detection cavities, the detection cavities are internally provided with electric connection interfaces, the electric connection interfaces are electrically connected with the display screen, the detection box is provided with an electric connection plug matched with the electric connection interfaces, and the image capture mechanism is electrically connected with the electric connection plug. The structure design can effectively improve the reaction and detection efficiency of the biological sample.

Biochip reaction mechanism include a transparent reaction disc, the reaction disc in be radial a plurality of reaction zones that are provided with, every along the circumference reaction zone include from inside to outside liquid pool and the reaction tank that sets gradually, liquid pool and reaction tank between be linked together through a pipe, the center and every of reaction disc the reaction zone in the center of liquid pool the center of reaction tank between the line with the central axis of pipe coincide mutually, the reaction disc on be provided with a plurality of sample adding holes, every the liquid pool on the intercommunication be provided with one the sample adding hole, the reaction disc on be provided with a plurality of exhaust holes, every the lateral part intercommunication of reaction tank be provided with one the exhaust hole. The biochip reaction mechanism can carry out multiple groups of reactions at the same time through the reaction areas arranged on the reaction disc, the operation efficiency is improved, each reaction area comprises the liquid pool and the reaction pool which are sequentially arranged from inside to outside, the liquid pool is communicated with the reaction pool through the conduit, the liquid pool is provided with the sample adding hole, the biological sample and the reaction reagent are added into the liquid pool through the sample adding hole, then a rotating force is applied to the reaction disc, the biological sample and the reaction reagent in the liquid pool can enter the reaction pool through the conduit to react under the action of the rotating force, other reagents are added in the process, the reagent is also added into the liquid pool through the sample adding hole, then the reagent enters the reaction pool through the conduit by applying the rotating force to the reaction disc, the structure is simple, the operation is convenient, the pollution of the biological sample in the reagent dripping process is effectively avoided, thereby obtain comparatively accurate experimental data, the setting up of exhaust hole makes biological sample and reaction reagent can enter into the reaction tank smoothly.

A non-return pool for preventing liquid from flowing back to the liquid pool is arranged between the liquid pool and the reaction pool, the guide pipe is divided into a first guide pipe section and a second guide pipe section, the liquid pool and the non-return pool are communicated through the first guide pipe section, the non-return pool and the reaction pool are communicated through the second guide pipe section, the connecting line between the center of the reaction disc and the center of the liquid pool, the center of the reaction pool and the center of the non-return pool in each reaction area is coincided with the central axis of the guide pipe, and the width of the non-return pool is larger than that of the guide pipe. The non-return pool with the structure can effectively prevent liquid in the reaction pool from flowing back to the liquid pool, thereby ensuring normal reaction, and having simple structure and stable use.

The detection box in be provided with a reaction chamber, the reaction chamber on be provided with a transparent chamber lid with overturning, the reaction chamber in be provided with a horizontal baffle, horizontal baffle will the reaction chamber separate for last cavity and lower cavity, the last cavity in be provided with the hot plate, horizontal baffle on be provided with and be used for shelving the seat of shelving of reaction disc, image capture mechanism be the camera, horizontal baffle on be provided with a shooting hole that link up from top to bottom, the camera just right the shooting hole be used for shooing the biological information on the reaction disc, the camera the hot plate with the light source all with the electricity connect the plug electricity and be connected, the light source setting be in the interior terminal surface of chamber lid be used for lighting up the last cavity. The reaction cavity is used for reaction of the reaction disc and detection after the reaction is finished, the cavity cover arranged on the reaction cavity is a transparent cavity cover which is convenient to observe, the reaction cavity is divided into an upper cavity and a lower cavity by a horizontal partition plate, the upper cavity is used for reaction, the lower cavity is used for installation of parts such as a camera and the like, and the structure is neat; the camera is used for capturing an image of the biological sample on the reaction disc after the reaction is completed, the heating plate is used for providing ambient temperature for the reaction of the biological sample, and the light source is used for providing illuminating light for shooting of the camera so as to obtain a clearer image of the biological sample.

The reaction disc is characterized in that a motor is arranged in the placing seat, the motor is electrically connected with the electric connecting plug, an output shaft of the motor vertically extends out of the placing seat, an installation column is fixedly connected onto the output shaft, an anti-rotation lug is convexly arranged on the lateral part of the installation column outwards, an installation hole which is matched with the installation column is coaxially arranged in the center of the reaction disc, an anti-rotation groove which is matched with the anti-rotation lug is arranged in the installation hole, and a placing disc is coaxially arranged at the bottom of the installation column. The motor is used for providing centrifugal force for the reaction disc, and simultaneously, the reaction disc is driven to rotate through the rotation of the motor during detection, and the information of all biological samples on one reaction disc can be completely captured through the camera; prevent changeing the lug and preventing the cooperation of changeing the recess, ensure that the reaction disc can follow motor output shaft synchronous rotation, shelve the disc and be used for playing the supporting role to the reaction disc.

The lower chamber is internally provided with a cooling fan, the cooling fan is electrically connected with the electric connecting plug, and the bottom of the detection box is provided with a cooling through groove. Through radiator fan's setting, the cooperation is dispelled the heat and is led to the groove for after the reaction, the temperature in the reaction chamber can fall back fast, forms the protection to the electrical component that sets up wherein.

The detection box body is internally provided with a circuit board, the circuit board is provided with a control circuit, the display screen and the electric connection interface are respectively and electrically connected with the control circuit, the detection box body is also provided with a USB interface, and the USB interface is electrically connected with the control circuit. The control circuit supplies power to the display screen; when the detection box is inserted into the detection box body, the electric connection interface and the correspondingly inserted electric connection socket are electrically communicated, and the rotation of the motor, the heating of the heating plate and the shooting of the camera are controlled by the control circuit; in addition, the USB interface can be used for transmitting picture data shot by the camera and supplying power to the control circuit by an external power supply.

The reaction cavity is internally provided with a temperature sensor, a temperature measuring probe of the temperature sensor extends into the upper cavity, and the temperature sensor is electrically connected with the electric connecting plug. The temperature sensor is used for detecting the temperature of the upper chamber, so that the control circuit is driven to control the heating plate to provide a proper reaction temperature.

The detection box body is internally provided with a rechargeable battery, the rechargeable battery is electrically connected with the control circuit, the detection box body is provided with a charging interface used for charging the rechargeable battery, and the charging interface is electrically connected with the rechargeable battery. The rechargeable battery is used for supplying power to the control circuit, and the interface that charges is used for charging rechargeable battery to make rechargeable battery supply power for whole equipment better, make the portability of whole device good.

Compared with the prior art, the utility model has the advantages of: the detection device integrates the reaction and the detection of the biochip in a detection box, after the reaction is completed, the light source is provided for the biochip when the detection is carried out, then the image information on the biochip is captured through the image capturing mechanism, the captured image information is transmitted to the image display mechanism, the structure is simple, the portability is good, and a relatively accurate observation result can be obtained.

drawings

FIG. 1 is a schematic structural view of a reaction disc of the present invention;

FIG. 2 is a schematic structural view of the reaction disk of the detection box of the present invention removed;

FIG. 3 is a schematic structural view of the detection cassette of the present invention with the biochip and the cover removed;

FIG. 4 is a schematic cross-sectional view of the detection box with the reaction disk installed thereon according to the present invention;

FIG. 5 is a schematic view of the front side of the detection box of the present invention;

FIG. 6 is a schematic view of a back side three-dimensional structure of the detection box of the present invention;

Fig. 7 is a schematic cross-sectional view of the detection box of the present invention inserted into the detection box.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments.

As shown in FIGS. 1 to 7, a qualitative/quantitative biochip measuring apparatus includes a measuring chamber B and a measuring cassette C, wherein an image display mechanism is disposed on the measuring chamber B, a biochip reaction mechanism, a light source (not shown) and an image capturing mechanism are disposed in the measuring cassette C, and the image capturing mechanism is electrically connected to the image display mechanism.

In this embodiment, an image display mechanism is disposed on the front end face of the inspection box B, the image display mechanism is a display screen B1, the rear end of the inspection box B is provided with a plurality of inspection cavities B2 matched with the inspection box C, the inspection box C is detachably inserted into the inspection cavity B2, an electrical connection interface B21 is disposed in the inspection cavity B2, an electrical connection interface B21 is electrically connected with the display screen B1, the inspection box C is provided with an electrical connection plug C1 matched with the electrical connection interface B21, and the image capture mechanism is electrically connected with the electrical connection plug C1.

In this embodiment, the biochip reaction mechanism includes a transparent reaction disk a1, a plurality of reaction areas a2 are radially arranged in the reaction disk a1 along the circumference, each reaction area a2 includes a liquid pool a21 and a reaction pool a22 which are sequentially arranged from inside to outside, the liquid pool a21 and the reaction pool a22 are communicated through a conduit, a connecting line between the center of the reaction disk a1 and the center of the liquid pool a21 and the center of the reaction pool a22 in each reaction area a2 coincides with the central axis of the conduit, a plurality of sample adding holes a11 are arranged on the reaction disk a1, one sample adding hole a11 is arranged on each liquid pool a21 in a communication, a plurality of vent holes a12 are arranged on the reaction disk a1, and a vent hole a12 is arranged on the side of each reaction pool a22 in communication.

In this particular embodiment, a check reservoir a23 for preventing liquid from flowing back into liquid reservoir a21 is provided between liquid reservoir a21 and reaction reservoir a22, the conduit is divided into a first conduit segment a24 and a second conduit segment a25, liquid reservoir a21 and check reservoir a23 communicate with each other through first conduit segment a24, check reservoir a23 and reaction reservoir a22 communicate with each other through second conduit segment a25, the line connecting the center of reaction disk a1 with the center of liquid reservoir a21, the center of reaction reservoir a22 and the center of check reservoir a23 in each reaction region a2 coincides with the central axis of the conduit, and the width of check reservoir a23 is greater than the width of the conduit.

in this embodiment, a reaction chamber is disposed in the detection box C, a transparent chamber cover C2 is disposed on the reaction chamber in a turnable manner, a horizontal partition C3 is disposed in the reaction chamber, the horizontal partition C3 divides the reaction chamber into an upper chamber C4 and a lower chamber C5, a heating plate C41 is disposed in the upper chamber C4, a placing seat C31 for placing the reaction disk a1 is disposed on the horizontal partition C3, the image capturing mechanism is a camera C8, a shooting hole C32 penetrating up and down is disposed on the horizontal partition C3, the camera C8 faces the shooting hole C32 for shooting biological information on the reaction disk a1, the camera C8, the heating plate C41, and a light source (not shown in the figure) are electrically connected to the electrical connection plug C1, and the light source is disposed on the inner end surface of the chamber cover C2 for illuminating the upper chamber C4.

In this embodiment, a motor C6 is disposed in the resting seat C31, the motor C6 is electrically connected to an electrical connection plug C1, an output shaft C61 of the motor C6 vertically extends out of the resting seat C31, an installation column C62 is fixedly connected to the output shaft C61, an anti-rotation protrusion C621 is disposed on a side portion of the installation column C62 in an outward protruding manner, an installation hole a13 which is cooperatively mounted with the installation column C62 is coaxially disposed in the center of the reaction disk A1, an anti-rotation groove a131 which is engaged with the anti-rotation protrusion C621 is disposed in the installation hole a13, and a resting disk C622 is coaxially disposed at the bottom of the installation column C62.

in this embodiment, a heat dissipation fan C51 is disposed in the lower chamber C5, the heat dissipation fan C51 is electrically connected to the electrical connection plug C1, and a heat dissipation through slot C9 is disposed at the bottom of the detection box C.

In this embodiment, a circuit board B6 is disposed in the detection box B, a control circuit is disposed on the circuit board B6, the display screen B1 and the electrical connection interface B21 are electrically connected to the control circuit, respectively, a USB interface B3 is further disposed on the detection box B, and a USB interface B3 is electrically connected to the control circuit.

In this embodiment, a temperature sensor C7 is disposed in the reaction chamber, a temperature probe of the temperature sensor C7 extends into the upper chamber C4, and the temperature sensor C7 is electrically connected to the electrical connection plug C1.

In this embodiment, a rechargeable battery B4 is disposed in the detection box B, the rechargeable battery B4 is electrically connected to the control circuit, a charging interface B5 for charging the rechargeable battery B4 is disposed on the detection box B, and the charging interface B5 is electrically connected to the rechargeable battery B4.

Claims

1. A qualitative/quantitative biochip tester is composed of a test box with image display unit, a biochip reaction unit, light source and image capture unit, and a test box with image display unit.

2. The device as claimed in claim 1, wherein the image display mechanism is disposed on the front surface of the detection box, the image display mechanism is a display screen, the back end of the detection box is provided with a plurality of detection cavities matching with the detection box, the detection box is detachably inserted into the detection cavities, the detection cavities are provided with electrical connection interfaces electrically connected with the display screen, the detection box is provided with electrical connection plugs matching with the electrical connection interfaces, and the image capture mechanism is electrically connected with the electrical connection plugs.

3. The qualitative/quantitative biochip measuring apparatus according to claim 2, wherein the biochip reaction mechanism comprises a transparent reaction disk, a plurality of reaction areas are radially arranged in the reaction disc along the circumference, each reaction area comprises a liquid pool and a reaction pool which are sequentially arranged from inside to outside, the liquid pool and the reaction pool are communicated through a conduit, the connecting line between the center of the reaction disc and the center of the liquid pool and the center of the reaction pool in each reaction area is coincident with the central axis of the conduit, the reaction disc is provided with a plurality of sample adding holes, each liquid pool is communicated with one sample adding hole, the reaction disc is provided with a plurality of exhaust holes, and the side part of each reaction tank is communicated with one exhaust hole.

4. The qualitative/quantitative biochip detecting apparatus according to claim 3, wherein a check reservoir for preventing liquid from flowing back to the liquid reservoir is disposed between the liquid reservoir and the reaction reservoir, the conduit is divided into a first conduit segment and a second conduit segment, the liquid reservoir and the check reservoir are communicated through the first conduit segment, the check reservoir and the reaction reservoir are communicated through the second conduit segment, a connecting line between a center of the reaction disk and a center of the liquid reservoir, a connecting line between a center of the reaction reservoir and a center of the check reservoir in each of the reaction regions coincides with a central axis of the conduit, and a width of the check reservoir is greater than a width of the conduit.

5. The qualitative/quantitative biochip measuring apparatus according to claim 3, wherein a reaction chamber is provided in the measuring cassette, a transparent cavity cover is arranged on the reaction cavity in a turnable way, a horizontal clapboard is arranged in the reaction cavity, the horizontal clapboard divides the reaction cavity into an upper cavity and a lower cavity, a heating plate is arranged in the upper cavity, the horizontal clapboard is provided with a placing seat for placing the reaction disc, the image capturing mechanism is a camera, the horizontal clapboard is provided with a shooting hole which is communicated up and down, the camera is right opposite to the shooting hole and is used for shooting the biological information on the reaction disc, the camera, the heating plate and the light source are electrically connected with the electric connecting plug, and the light source is arranged on the inner end face of the cavity cover and used for illuminating the upper cavity.

6. The qualitative/quantitative biochip detecting device according to claim 5, wherein a motor is disposed in the holder, the motor is electrically connected to the electrical connecting plug, an output shaft of the motor vertically extends out of the holder, a mounting post is fixedly connected to the output shaft, an anti-rotation protrusion is protruded outwardly from a side of the mounting post, a mounting hole is coaxially disposed in the center of the reaction disk, the mounting hole is disposed therein, an anti-rotation groove is disposed therein, the anti-rotation groove is engaged with the anti-rotation protrusion, and a holder disk is coaxially disposed at the bottom of the mounting post.

7. The qualitative/quantitative biochip measuring apparatus according to claim 5, wherein a heat dissipation fan is disposed in the lower chamber, the heat dissipation fan is electrically connected to the electrical connection plug, and a heat dissipation channel is disposed at the bottom of the measuring chamber.

8. the qualitative/quantitative biochip measuring device according to claim 5, wherein the measuring chamber has a circuit board disposed thereon, the circuit board having a control circuit, the display and the electrical connection interface being electrically connected to the control circuit, the measuring chamber further having a USB interface electrically connected to the control circuit.

9. The qualitative/quantitative biochip measuring device according to claim 5, wherein a temperature sensor is disposed in the reaction chamber, a temperature probe of the temperature sensor extends into the upper chamber, and the temperature sensor is electrically connected to the electrical connector.

10. The device as claimed in claim 8, wherein a rechargeable battery is disposed in the detection housing, the rechargeable battery is electrically connected to the control circuit, and a charging port for charging the rechargeable battery is disposed in the detection housing and electrically connected to the rechargeable battery.