CN109613288A - A kind of qualitative/quantitative biological chips detection system - Google Patents
A kind of qualitative/quantitative biological chips detection system Download PDFInfo
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- CN109613288A CN109613288A CN201811607091.XA CN201811607091A CN109613288A CN 109613288 A CN109613288 A CN 109613288A CN 201811607091 A CN201811607091 A CN 201811607091A CN 109613288 A CN109613288 A CN 109613288A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00158—Elements containing microarrays, i.e. "biochip"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)
- G01N2035/00287—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material) movable lid/cover for sample or reaction tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of qualitative/quantitative biological chips detection systems, feature be include loading box, loading box includes that cabinet and lid close the lid being arranged on cabinet, accommodating chamber is provided in cabinet, multiple containing cavities are provided in accommodating chamber, detection carrier, enriching apparatus, liquid-transfering device, detection device and the reagent storage box for being placed with detection reagent are accommodated in multiple containing cavities respectively, is provided with handle on cabinet.Advantage is to integrate each equipment for being used for biochip test to be placed in the loading box of an entirety, neat and small in size, while handle is installed in the loading box, and easy to remove and carrying, structure is simple, easy to use.
Description
Technical field
The present invention relates to biochip test fields, more particularly, to a kind of qualitative/quantitative biological chips detection system.
Background technique
Biochip (biochip or bioarray) be according to the principle specifically to interact between biomolecule, will be biochemical
Analytic process is integrated in chip surface, to realize the high throughput to DNA, RNA, polypeptide, protein and other biological ingredient
Quickly detection.The biochip concept of narrow sense refers to biomolecule (oligonucleotides, cDNA, genomic through distinct methods
DNA, polypeptide, antibody, antigen etc.) it is bonded in the solid phase mediators such as silicon wafer, sheet glass (pearl), plastic sheet (pearl), gel, nylon membrane
The biomolecule dot matrix of formation.The concept of chip takes from integrated concept, and biochip is the integrated of biomaterial.As experiment
Room detection is the same, and blood glucose, albumen, enzymatic activity etc. are checked on biochip, is based on same biological respinse principle.So raw
Object chip is exactly a carrier platform, is a carrier, is that analytic process discontinuous in life science is integrated in base
The miniature biochemical analysis system on piece surface, to realize to the accurate, fast of cell, protein, gene and other biological components
The detection of speed, large information capacity.The technical advantage that biochip has keeps detection quicker, sensitive and accurate.Conventional biology
Chip detection is needed with equipment such as nucleic acid augmentative instrument, chip hybridization instrument, chip cleaner and detectors.Currently on the market above-mentioned
A whole set of related equipment is not only expensive, and volume is big and heavy, should not often move, and mobile difficult larger.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of qualitative/quantitative biochips small in size and easy to remove
Detection system.
The technical scheme of the invention to solve the technical problem is:
A kind of qualitative/quantitative biological chips detection system, including loading box, the loading box include that cabinet and lid close setting and exist
Lid on the cabinet is provided with accommodating chamber in the cabinet, multiple containing cavities is provided in the accommodating chamber, more
Detection carrier, enriching apparatus, liquid-transfering device, detection device are accommodated in a containing cavities respectively and is placed with detection reagent
Reagent storage box, be provided with handle on the cabinet.
The enriching apparatus includes test tube and magnetic separation rack, and immunomagnetic beads are provided in the test tube, described
Magnetic separation rack include it is horizontally disposed shelve plate and shelve bottom plate, it is described to shelve plate and shelve between bottom plate by vertical
Support plate support is fixed, and is provided with magnet in the support plate, and described shelving is arranged on plate multiple to be had and the test tube
What is matched shelves through-hole, and the test tube is shelved on described shelve in through-hole.Above-mentioned enriching apparatus structure is simple, can be fast
Speed is effectively enriched to biological sample to be checked.
The liquid-transfering device includes pipettor and liquid transfer gun head.Pipettor and liquid transfer gun head cooperation, use for liquid relief.
The cabinet includes bottom plate, front side board and back side panel, and the front side board and back side panel difference are vertical
The two sides of the front-end and back-end of bottom plate described in being disposed around, the front side board and the back side panel pass through vertically arranged respectively
Left plate be connected with right side plate, the left plate and the right side plate are arranged on the bottom plate, the bottom
Plate, front side board, back side panel, left plate and right side plate, which enclose, is set as the accommodating chamber, and the front side is arranged in the handle
On plate, the lid is a horizontally disposed cover board, be provided on the inner wall of the front side board and the back side panel with
The slippage slot that the cover board matches, the rear and front end of the cover board, which can control, is slippingly arranged in the slippage slot
It is interior.Above-mentioned body structure is simple, and opening and closing are realized in cover board or so sliding, easy to operate and laborsaving.
The detection carrier is biochip, and the biochip includes a transparent reaction disk, and described is anti-
It answers and is circumferentially radially provided with multiple conversion zones in disk, each conversion zone includes successively setting from inside to outside
The liquid cell and reaction tank set are connected between the liquid cell and reaction tank by a conduit, the reaction disk
Center and the center of the liquid cell in the conversion zone, the line between the center of the reaction tank with
The central axis of the conduit coincides, and is provided with multiple wells on the reaction disk, each liquid cell
On be provided with and communicated with one described in well, be provided with multiple gas vents on the reaction disk, each reaction
The side in pond be provided with and communicated with one described in gas vent.By the multiple conversion zones being arranged on reaction disk, so that should
Biochip can carry out multiple groups reaction in the same time, improve operating efficiency, and each conversion zone include from inside to outside according to
The liquid cell and reaction tank of secondary setting, are connected between liquid cell and reaction tank by a conduit, and sample-adding is provided on liquid cell
Hole, biological sample and reaction reagent are added in liquid cell by well, then apply a rotatory force, liquid to reaction disk
Biological sample and reaction reagent in body pond can enter reaction tank by conduit under the action of the rotatory force and be reacted, and
Other reagents are added in the process, then the reagent are equally added to liquid cell by well, then by giving reaction circle
Disk applies rotatory force, so that the reagent is entered in reaction tank by conduit, structure is simple, easy to operate, effectively prevents giving birth to
Object sample is contaminated during reagent dropwise, to obtain more accurately experimental data, the setting of gas vent is so that biology
Sample and reaction reagent can smoothly enter into reaction tank.
It is provided between the liquid cell and the reaction tank for preventing liquid from flowing back to stopping for the liquid cell
Hui Chi, the conduit are divided into the first catheter segment and the second catheter segment, pass through between the liquid cell and the non-return pond
First catheter segment is connected, and is connected between the non-return pond and the reaction tank by second catheter segment
It is logical, the center of the reaction disk with each described in conversion zone in the liquid cell center, described react
The central axis of line and the conduit between the center in pond and the center in the non-return pond coincides, the non-return
The width in pond is greater than the width of the conduit.The liquid that the setting in the non-return pond of above structure can be effectively prevent in reaction tank returns
It flows in liquid cell, so that it is guaranteed that reaction is normally carried out, structure is simple, uses stabilization.
The detection device includes biochip reaction case, and the biochip reaction case includes reaction cabinet and can
Lid closes the case lid being arranged on the reaction cabinet, and one is provided in the reaction chamber body and is matched with the disk that reacts
The cylindric reaction chamber of conjunction, is provided with a horizontal baffle in the reaction chamber, and the horizontal baffle is by the reaction
Chamber is divided into upper chamber and lower chambers, and the case lid lid conjunction is arranged in the upper chamber, is arranged in the case lid
It is provided with the light source for illuminating the upper chamber, heating sheet is provided on the inner sidewall of the reaction chamber, under described
Motor is provided in chamber, the output shaft of the motor is extend into vertically upward in the upper chamber, the reaction circle
The central coaxial of disk is provided with the mounting hole being coupled with the output shaft, and the bottom chamber is provided with camera,
A camera hole up and down, camera hole described in the camera face are provided on the horizontal baffle.Above-mentioned life
Object chip reaction box structure is simple, and the intracorporal reaction chamber of reaction chamber is divided into upper chamber and lower chambers by horizontal baffle, on
Chamber is used for the reaction of biochip, and lower chambers are used for the installation of the components such as camera, motor, and structure is neat；Pass through motor
Rotation provide a centrifugal force to reaction disk, by the effect of centrifugal force so that biological sample and reaction in liquid cell
Reagent quickly and steadily can be entered in reaction tank and be reacted, easy to operate and quick, while by the rotation of motor,
Drive detection chip rotation so that each conversion zone can camera-shot arrive, to obtain the letter of multiple groups biological sample
Breath.
A mounting post is fixedly connected on the output shaft, the side outwardly convex of the mounting post is provided with anti-rotation
Convex block is provided with the anti-rotation groove matched with the anti-rotation convex block, the bottom of the mounting post in the mounting hole
It is coaxially arranged with one and shelves disk.It is installed on output shaft with mounting post for installing reaction disk, structure is simple, installation side
Just, pass through the cooperation of anti-rotation groove and anti-rotation convex block, it is ensured that when react disk install after can as output shaft rotates together,
To which offer keeps biological sample and reaction reagent stable from liquid cell and is rapidly introduced into the centrifugal force into reaction tank；It shelves
Disk provides lower limit for shelving biochip, to the installation of biochip.
The side of the reaction cabinet is provided with electric appliance box, and circuit board and chargeable is provided in the electric appliance box
Battery, control circuit is laid on the circuit board, and the battery is electrically connected for described with the control circuit
Control circuit power supply, the heating sheet, the light source and the camera are electrically connected with the control circuit respectively
It connects, display screen is provided on the electric appliance box, the display screen is electrically connected with the control circuit, the electric appliance box
On be additionally provided with USB interface, the USB interface is electrically connected with the control circuit.Control circuit is for controlling heating sheet
Heating, light source switch, the shooting of camera and motor rotation etc., enable entire detection device normal use, USB
Interface is used for the transmission of data.
A temperature sensor is provided in the reaction chamber body, the temperature probe of the temperature sensor protrudes into setting
In the upper chamber, the temperature sensor is electrically connected with the control circuit.Temperature sensor is anti-for detecting
The intracorporal temperature of case is answered, so that control circuit control heating sheet be driven to provide more suitable reaction temperature.
The case lid lower end capping is provided with it is multiple heat dissipation through slots heat sink, the heat sink with it is described
Case lid between enclose and be set as a heat dissipation cavity, be provided with radiator fan in the heat dissipation cavity, the radiator fan and described
Control circuit is electrically connected, and is provided with multiple heat release holes up and down on the case lid.By above-mentioned radiator structure for giving
Cabinet cooling is reacted, structure is simple, good heat dissipation effect.
In the circumferential wall of the end to end inside for being disposed around the reaction chamber of the heating sheet, the heating sheet
Outside is uniformly arranged a plurality of vertical through slot round there is anti-scald ring on the anti-scald ring.Anti-scald ring avoids grasping for anti-scald
Author is heated piece scald will test chip and be placed into reaction chamber, so that entire detection device is used safely.
It is provided with the first magnet on the reaction cabinet, is provided with the second magnet on the case lid, described first
Magnet is corresponding up and down with the position of second magnet, first magnet, two ends opposite with second magnet
Face magnetic pole is opposite.The covering structure of above-mentioned case lid and reaction chamber is simple, folding with convenience.
It is additionally provided with mobile power source in the cabinet, multiple USB interfaces are provided on the right side plate, it is described
USB interface is electrically connected with the mobile power source.Mobile power source is used to charge to the intracorporal electrical equipment of case, is arranged on right side plate
USB interface for external power supply give mobile power source charging.
The right side of cover board is downwardly vertical to be bent to form a protection board, when the lid covers conjunction on the cabinet completely
When, the protection board lid is located at the side of the right side plate.Protection board is for meeting the above-mentioned USB being arranged on right side plate
Mouth shields.
Compared with the prior art, the advantages of the present invention are as follows: each equipment for being used for biochip test is integrated and is put
It sets in the loading box of an entirety, it is neat and small in size, while handle being installed in the loading box, and easy to remove and carrying, knot
Structure is simple, easy to use.
Detailed description of the invention
Fig. 1 is the overall structure diagram in the present invention；
Fig. 2 is the structural schematic diagram that lid of the present invention is opened；
Fig. 3 is decomposition texture schematic diagram of the invention；
Fig. 4 is the structural schematic diagram of biochip in the present invention；
Fig. 5 is the structural schematic diagram of detection device in the present invention；
Fig. 6 is the structural schematic diagram that the case lid of detection device in the present invention is opened；
Fig. 7 is the schematic cross-sectional view of detection device in the present invention.
Specific embodiment
The present invention will be described in further detail below with reference to the embodiments of the drawings.
As shown in Figures 1 to 7, a kind of qualitative/quantitative biological chips detection system, including loading box C, loading box C include
Cabinet C1 and lid close the lid being arranged on cabinet C1, are provided with accommodating chamber in cabinet C1, multiple storages are provided in accommodating chamber
Detection carrier, enriching apparatus, liquid-transfering device, detection device are accommodated in chamber C11, multiple containing cavities C11 respectively and is placed with inspection
Handle C12 is provided on reagent the storage box E, cabinet C1 of test agent.
In this particular embodiment, enriching apparatus includes test tube (not shown) and magnetic separation rack D1, and test tube is placed
In test tube storage box D2, it is provided with immunomagnetic beads in test tube, magnetic separation rack D1 includes horizontally disposed shelving plate D11 and putting
Bottom set plate D12 shelves plate D11 and shelves and fixed between bottom plate D12 by vertical support plate D13 support, set on support plate D13
Be equipped with magnet (not shown), shelve be arranged on plate D11 it is multiple have shelve through-hole D111 with what test tube matched, test tube is shelved
It is shelving in through-hole D111.
In this particular embodiment, liquid-transfering device includes pipettor F and liquid transfer gun head (not shown), and liquid transfer gun head is put
It sets in liquid transfer gun head storage box F1.
In this particular embodiment, cabinet C1 includes bottom plate (not shown), front side board C16 and back side panel C13, front side
Plate C16 and back side panel C13 is vertically disposed around the front-end and back-end of bottom plate respectively, and the two sides of front side board C16 back side panel C13 lead to respectively
Vertically arranged left plate C14 is crossed to be connected with right side plate C15, left plate C14 and right side plate C15 are arranged on bottom plate, bottom plate,
Front side board C16, back side panel C13, left plate C14 and right side plate C15, which enclose, is set as accommodating chamber, and handle C12 is arranged in front side board C16
On, lid matches to be provided on the inner wall of a horizontally disposed cover board C2, front side board C16 and back side panel C13 with cover board C2
Slippage slot C3, the rear and front end of cover board C2, which can control, to be slippingly arranged in slippage slot C3.
In this particular embodiment, detection carrier is biochip A, and biochip A includes a transparent reaction disk A1,
Multiple conversion zone A2 are circumferentially radially provided in reaction disk A1, and each conversion zone A2 includes from inside to outside successively
The liquid cell A21 and reaction tank A22 of setting, are connected between liquid cell A21 and reaction tank A22 by a conduit, and disk is reacted
The center of A1 and the center of the liquid cell A21 in each conversion zone A2, reaction tank A22 center between line and conduit
Central axis coincides, and reacts and is provided with multiple well A11 on disk A1, is provided with and communicated with one on each liquid cell A21
Well A11 reacts and is provided with multiple gas vent A12 on disk A1, and the side of each reaction tank A22 is provided with and communicated with a row
Stomata A12.
In this particular embodiment, it is provided between liquid cell A21 and reaction tank A22 for preventing liquid from flowing back to liquid
The non-return pond A23 of pond A21, conduit are divided into the first catheter segment A24 and the second catheter segment A25, liquid cell A21 and non-return pond A23 it
Between be connected by the first catheter segment A24, be connected between non-return pond A23 and reaction tank A22 by the second catheter segment A25, instead
The center for answering disk A1 and the center of liquid cell A21, the center of reaction tank A22 and non-return pond A23 in each conversion zone A2
Center between line and the central axis of conduit coincide, the width of non-return pond A23 is greater than the width of conduit.
In this particular embodiment, detection device includes biochip reaction case B, and biochip reaction case B includes reaction
Cabinet B1 and Ke Gai close the case lid B2 being arranged on reaction cabinet B1, are provided with one in reaction cabinet B1 and match with disk A1 is reacted
The cylindric reaction chamber of conjunction is provided with a horizontal baffle B12 in reaction chamber, and reaction chamber is divided into epicoele by horizontal baffle B12
Room B11 and lower chambers B13, case lid B2 lid, which close, to be arranged on upper chamber B11, is provided in case lid B2 for illuminating upper chamber B11
Light source (not shown), be provided with heating sheet B3 on the inner sidewall of reaction chamber, motor B4 be provided in lower chambers B13, electricity
The output shaft B41 of machine B4 is extend into vertically upward in upper chamber B11, and the central coaxial of reaction disk A1 is provided with and output shaft
It is provided with camera B5 in the mounting hole A13 that B41 is coupled, lower chambers B13, is provided with about one on horizontal baffle B12 and passes through
Logical camera hole B51, camera B5 face camera hole B51.Above-mentioned biochip reaction case B structure is simple, passes through horizontal baffle
The reaction chamber reacted in cabinet B1 is divided into upper chamber B11 and lower chambers B13 by B12, and upper chamber B11 is for biochip A's
Reaction, installation of the lower chambers B13 for components such as camera B5, motor B4, structure are neat；By the rotation of motor B4 to life
Object chip A provides a centrifugal force, enables biological sample and reaction reagent in liquid cell A21 by the effect of centrifugal force
It reaches quickly and steadily enters in reaction tank A22 and reacted, it is easy to operate and quick, while passing through the rotation of motor B4,
Biochip A rotation is driven, so that each conversion zone A2 can be taken by camera B5, to obtain multiple groups biological sample
Information.
In this particular embodiment, be fixedly connected with a mounting post B414 on output shaft B41, the side of mounting post B414 to
Outer lug is provided with anti-rotation convex block B4141, and the anti-rotation groove matched with anti-rotation convex block B4141 is provided in mounting hole A13
A131；Disk B4142 is shelved for shelving biochip A, provides lower limit to the installation of biochip A.On output shaft B41
With mounting post B414 for installing reaction disk A1, structure is simple for installation, easy for installation, convex by anti-rotation groove A131 and anti-rotation
The cooperation of block B4141, it is ensured that can be as output shaft B41 is rotated together, so that providing makes to give birth to after reacting disk A1 and installing
Object sample and reaction reagent from liquid cell A21 stabilization and are rapidly introduced into the centrifugal force in reaction tank A22.
In this particular embodiment, the side for reacting cabinet B1 is provided with electric appliance box B6, is provided with circuit in electric appliance box B6
It plate B63 and rechargeable battery B62, control circuit (not shown), battery B62 control circuit be laid on circuit board B63
Electrical connection to control circuit for powering, and heating sheet B3, light source and camera B5 are electrically connected with control circuit respectively, electric appliance box B6
On be provided with display screen B61, display screen B61 is electrically connected with control circuit, and USB interface is additionally provided on electric appliance box B6 (in figure not
Display), USB interface is electrically connected with control circuit.Switch of the control circuit for controlling heating sheet B3 heating, light source, photography
The shooting of head and motor B4 rotation etc., enable entire detection device normal use, USB interface is used for the transmission of data.
In this particular embodiment, it reacts in cabinet B1 and is provided with a temperature sensor B14, the survey of temperature sensor B14
Temperature probe, which protrudes into, to be arranged in upper chamber B11, and temperature sensor B14 is electrically connected with control circuit.Temperature sensor B14 is for anti-
The temperature in cabinet B1 is answered, so that control circuit control heating sheet B3 be driven to provide more suitable reaction temperature.
In this particular embodiment, the lower end capping of case lid B2 is provided with the heat sink B21 with multiple heat dissipation through slots, dissipates
It is enclosed between hot plate B21 and case lid B2 and is set as a heat dissipation cavity B22, radiator fan B23, radiator fan B23 are provided in heat dissipation cavity B22
It is electrically connected with control circuit, multiple heat release hole B24 up and down is provided on case lid B2.By above-mentioned radiator structure for giving
Cabinet B1 cooling is reacted, structure is simple, good heat dissipation effect.
In this particular embodiment, in the circumferential wall of the end to end inside for being disposed around reaction chamber heating sheet B3, heating sheet
The outside of B3 is uniformly arranged a plurality of vertical through slot B311 round there is anti-scald ring B31 on anti-scald ring B31.Anti-scald ring B31 is used for
It is anti-scald, avoid operator from being heated piece B3 scald in biochip A to be placed into reaction cabinet B1, so that entire detection device
It is used safely.
In this particular embodiment, one end of case lid B2 is rotatably arranged on reaction cabinet B1, is reacted on cabinet B1
It is provided with the first magnet B 7, the other end of case lid B2 is provided with the second magnet B 8, the position of the first magnet B 7 and the second magnet B 8
Corresponding up and down, the first magnet B 7 is opposite with two opposite end face magnetic poles of the second magnet B 8.Above-mentioned case lid B2 and reaction cabinet B1
Covering structure it is simple, folding with convenience.
In this particular embodiment, it is additionally provided with mobile power source G in cabinet C1, multiple USB are provided on right side plate C15 and are connect
Mouthful G1, USB interface G1 are electrically connected with mobile power source G.
In this particular embodiment, the right side of cover board C2 is downwardly vertical is bent to form a protection board C21, when lid covers completely
It closes when on cabinet C1, protection board C21 covers the side for being located at right side plate C15.
Claims (10)
1. a kind of qualitative/quantitative biological chips detection system, it is characterised in that including loading box, the loading box includes cabinet
The lid being arranged on the cabinet is closed with lid, accommodating chamber is provided in the cabinet, is provided in the accommodating chamber
Multiple containing cavities, be accommodated in multiple containing cavities respectively detection carrier, enriching apparatus, liquid-transfering device, detection device and
It is placed with the reagent storage box of detection reagent, is provided with handle on the cabinet.
2. a kind of qualitative/quantitative biological chips detection system as described in claim 1, it is characterised in that the cabinet includes
Bottom plate, front side board and back side panel, the front side board and the back side panel are vertically disposed around the front end of the bottom plate respectively
And rear end, the front side board pass through vertically arranged left plate with the two sides of the back side panel respectively and are connected with right side plate
It connects, the left plate and the right side plate are arranged on the bottom plate, the bottom plate, front side board, back side panel, left side
Plate and right side plate, which enclose, is set as the accommodating chamber, and the handle is arranged on the front side board, and the lid is a water
The sliding matched with the cover board is provided on the inner wall of the cover board of flat setting, the front side board and the back side panel
Slot, the rear and front end of the cover board, which can control, to be slippingly arranged in the slippage slot.
3. a kind of qualitative/quantitative biological chips detection system as described in claim 1, it is characterised in that the detection carrier
For biochip, the biochip includes a transparent reaction disk, in the reaction disk circumferentially radially
Multiple conversion zones are provided with, each conversion zone includes the liquid cell and reaction tank set gradually from inside to outside, institute
It is connected between the liquid cell and reaction tank stated by a conduit, the center of the reaction disk and each reaction zone
The central axis phase of line and the conduit between the center of the liquid cell in domain, the center of the reaction tank
It is overlapped, is provided with multiple wells on the reaction disk, be provided with and communicated with described in one on each liquid cell
Well is provided with multiple gas vents on the reaction disk, and the side of each reaction tank is provided with and communicated with one
The gas vent；
The non-return pond for preventing liquid from flowing back to the liquid cell is provided between the liquid cell and the reaction tank,
The conduit is divided into the first catheter segment and the second catheter segment, by described between the liquid cell and the non-return pond
First catheter segment is connected, and is connected between the non-return pond and the reaction tank by second catheter segment, institute
In the center of the reaction disk stated and the center of the liquid cell in the conversion zone, the reaction tank
The central axis of line and the conduit between the heart and the center in the non-return pond coincides, the width in the non-return pond
Degree is greater than the width of the conduit.
4. a kind of qualitative/quantitative biological chips detection system as claimed in claim 3, it is characterised in that the detection device
Including biochip reaction case, the biochip reaction case includes that the reaction chamber is arranged in reaction cabinet and Ke Gai conjunction
Case lid on body is provided with one and the cylindric reaction chamber for reacting disk and matching, institute in the reaction chamber body
A horizontal baffle is provided in the reaction chamber stated, the reaction chamber is divided into upper chamber and cavity of resorption by the horizontal baffle
Room, the described case lid lid conjunction are arranged in the upper chamber, be arranged in be provided in the case lid it is described for illuminating
The light source of upper chamber is provided with heating sheet on the inner sidewall of the reaction chamber, and the bottom chamber is provided with motor, described
The output shaft of motor extend into the upper chamber vertically upward, the central coaxial of the reaction disk is provided with and institute
The mounting hole that the output shaft stated is coupled, the bottom chamber are provided with camera, are provided on the horizontal baffle
One camera hole up and down, camera hole described in the camera face.
5. a kind of qualitative/quantitative biological chips detection system as claimed in claim 4, it is characterised in that on the output shaft
It is fixedly connected with a mounting post, the side outwardly convex of the mounting post is provided with anti-rotation convex block, sets in the mounting hole
It is equipped with the anti-rotation groove matched with the anti-rotation convex block, the bottom of the mounting post is coaxially arranged with one and shelves disk.
6. a kind of qualitative/quantitative biological chips detection system as claimed in claim 4, it is characterised in that the reaction cabinet
Side be provided with electric appliance box, being provided in the electric appliance box circuit board and rechargeable battery, cloth on the circuit board
Equipped with control circuit, the battery is electrically connected for powering to the control circuit with the control circuit, described
Heating sheet, the light source and the camera are electrically connected with the control circuit respectively, are arranged on the electric appliance box
There is display screen, the display screen is electrically connected with the control circuit, and USB interface, institute are additionally provided on the electric appliance box
The USB interface stated is electrically connected with the control circuit.
7. a kind of qualitative/quantitative biological chips detection system as claimed in claim 6, it is characterised in that the reaction cabinet
It is inside provided with a temperature sensor, the temperature probe of the temperature sensor, which protrudes into, to be arranged in the upper chamber, described
Temperature sensor be electrically connected with the control circuit.
8. a kind of qualitative/quantitative biological chips detection system as claimed in claim 6, it is characterised in that under the case lid
End seal lid is provided with the heat sink with multiple heat dissipation through slots, encloses between the heat sink and the case lid and is set as a heat dissipation
Chamber is provided with radiator fan in the heat dissipation cavity, and the radiator fan is electrically connected with the control circuit, the case
It covers and is provided with multiple heat release holes up and down.
9. a kind of qualitative/quantitative biological chips detection system as claimed in claim 2, it is characterised in that in the cabinet also
It is provided with mobile power source, multiple USB interfaces, the USB interface and the mobile power source are provided on the right side plate
Electrical connection.
10. a kind of qualitative/quantitative biological chips detection system as claimed in claim 9, it is characterised in that the right side of cover board to
It is vertically bent to form a protection board down, when the lid covers conjunction on the cabinet completely, the protection board lid is set
In the side of the right side plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811607091.XA CN109613288A (en) | 2018-12-27 | 2018-12-27 | A kind of qualitative/quantitative biological chips detection system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811607091.XA CN109613288A (en) | 2018-12-27 | 2018-12-27 | A kind of qualitative/quantitative biological chips detection system |
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| CN109613288A true CN109613288A (en) | 2019-04-12 |
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