CN209619353U - The device of environment DNA in a kind of collection seawater - Google Patents
The device of environment DNA in a kind of collection seawater Download PDFInfo
- Publication number
- CN209619353U CN209619353U CN201920125138.2U CN201920125138U CN209619353U CN 209619353 U CN209619353 U CN 209619353U CN 201920125138 U CN201920125138 U CN 201920125138U CN 209619353 U CN209619353 U CN 209619353U
- Authority
- CN
- China
- Prior art keywords
- support column
- column
- filter
- seawater
- environment dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a kind of device of environment DNA in collection seawater, support column is installed in Filter column, Filter column inside is divided into two spaces by support column, and the space inside support column is equipped with filter membrane, and multiple air vents are offered on support column；One end of Filter column offers the water inlet being connected with support column inner space, water inlet is connected by hose with peristaltic pump, the other end of Filter column offers the water outlet that the space between support column and Filter column is connected, and water outlet is received with seawater and is connected to inside container；Seawater is evacuated in the space inside support column by peristaltic pump through hose, collects environment DNA by membrane filtration, filtered seawater enters the space between support column and Filter column through air vent, and then drains into seawater by water outlet and receive container.The utility model can effectively can avoid sample from toppling over and pollute in efficient, convenient collection seawater while environment DNA, and safe operation, simple, convenient for saving.
Description
Technical field
The utility model belongs to environment DNA field, specifically a kind of device for collecting environment DNA in seawater.
Background technique
Environment DNA, refer to biology fall off tissue in the environment or contain into the cell DNA (Ficetola et al,
2008).By acquiring environment DNA sample, it is based on existing genetic analysis method, so that it may detect occur in survey area
The biological species crossed.
Environment DNA technology is efficient one kind, low cost, to the undamaged technology of environment, the life carried out based on environment DNA
State monitoring not only can guarantee the destruction and interference that collects effective sample but also can be avoided to biological habitat, be domestic and international research heat
Point field.But environment DNA is easily degraded and content is lower in briny environment, in existing research, it is common to use filter membrane method into
The collection of row environment DNA (Jiang Wei etc., 2016；Doi,et al,2017；Li Miao etc., 2018).Filter membrane method collects process, and there are following
Disadvantage: (1) seawater sample need to be filled manually, labor intensive cost, and easily cause water sample to topple in operating process；(2) filter membrane is complete
It is exposed in air, is easy when sampling by cross contamination between source of people pollution or sample entirely；(3) filter membrane opening is wide, easily causes
Environment DNA degradation；(4) filter membrane belongs to easily broken consumptive material, is easily damaged；(5) after collecting environment DNA sample, filter membrane need to be placed in
It is saved in liquid nitrogen container, there is risk.
Bibliography:
Li Miao, Dan Xiujuan, Wang Weiji, Lv Ding, Dai Fangqun, Ding little Song, Wu Huanhuan.The environment of Chinese prawn biomass evaluation
The foundation and optimization of DNA detection technique.Fishery science progress, 2018.
Jiang Wei, Wang Qijun, Deng Jie, Zhao Hu, Kong Fei, Zhang Hongxing.Using Hucho bleekeri as the water sample environment DNA of target species
The optimization of analysis process.Chinese Journal of Applied Ecology, 2016,27:2372-2378.
Doi H,Inui R,Akamatsu Y,Kanno K,et al.Environmental DNA analysis for
estimating the abundance and biomass of stream fish.Freshwater Biology,2017,
62:30-39.
Ficetola GF,Miaud C,Pompanon F,et al.Species detection using
environmental DNA from water samples.Biology letters,2008,4:423-425.
Utility model content
In order to evade the loss easy to pollute, degradable, easy of environment DNA caused by filter membrane method, not easy to maintain and high labor cost
The disadvantages of, the utility model provides a kind of device for collecting environment DNA in seawater.The device can not only efficiently, simply acquire ring
Border DNA sample, and can prevent sample from toppling over or polluting, it is firm and convenient for saving, it saves labour turnover.
The purpose of this utility model is achieved through the following technical solutions:
The utility model includes Filter column, support column and filter membrane, is wherein equipped with support column in Filter column, in the Filter column
Portion is divided into two spaces by the support column, and the space inside the support column is equipped with filter membrane, offers on the support column
Multiple air vents；One end of the Filter column offers the water inlet being connected with support column inner space, which passes through
Hose is connected with peristaltic pump, and what the other end of the Filter column offered that the space between support column and Filter column is connected goes out
The mouth of a river, the water outlet are received with seawater and are connected to inside container；Seawater is evacuated to the sky inside support column through hose by the peristaltic pump
Between in, environment DNA is collected by the membrane filtration, filtered seawater enters the support column and mistake through the air vent
Space between filter column, and then seawater is drained by the water outlet and receives container；
Wherein: the filter membrane is abutted with the inner wall of support column；
The Filter column is divided into the upper cover and cylinder of threaded connection sealed against one another, one end of the support column removably with
The inner surface of upper cover connects；
The water inlet, water outlet, the longitudinal center line of Filter column and support column are conllinear.
The advantages of the utility model and good effect are as follows:
1. the utility model effectively can avoid sample from inclining in efficient, convenient collection seawater while environment DNA
And pollution, and it is safe operation, simple, convenient for saving.
2. the utility model is connect by Filter column with peristaltic pump, efficiently, simply, safely by the environment DNA in seawater
It collects, and is easy to save after collecting, environment DNA quality is high.
Detailed description of the invention
Fig. 1 is the structure sectional view of the utility model；
Fig. 2 is the working state figure of the utility model；
Wherein: 1 is Filter column, and 2 be cylinder, and 3 be support column, and 4 be filter membrane, and 5 be water inlet, and 6 be water outlet, and 7 be ventilation
Hole, 8 be upper cover, and 9 be hose, and 10 receive container for seawater.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing.
As shown in Figure 1, the utility model includes Filter column 1, support column 3 and filter membrane 4, branch wherein is installed in Filter column 1
Dagger 3 is divided for two spaces by support column 3 inside the Filter column 1, and the space inside the support column 3 is equipped with filter membrane 4, filter
Film 4 is abutted with the inner wall of support column 3, and multiple air vents 7 are offered on support column 3；It is close each other that the Filter column 1 of the present embodiment, which divides,
It seals the upper cover 8 being threadedly coupled and cylinder 2, one end of support column 3 is removably connect with the inner surface of upper cover 8, in order to install
Or replacement filter membrane 4.
One end of Filter column 1 offers the water inlet 5 being connected with 3 inner space of support column, which passes through hose
9 are connected with peristaltic pump；The other end of Filter column 1 offers the water outlet that the space between support column 3 and Filter column 1 is connected
6, which receives with seawater and is connected to inside container 10.The water inlet 5, water outlet 6, Filter column 1 and support column of the present embodiment
3 longitudinal center line is conllinear, and water inlet 5 is blocked when not working with rubber plug with water outlet 6.
The working principle of the utility model are as follows:
The utility model is cooperated compacted using closed, unidirectional disengaging, included filter membrane 4 and the Filter column 1 for having air vent 3
Dynamic pump uses, and can efficiently collect the environment DNA in seawater under the premise of non-staining.As shown in Fig. 2, concrete operations are as follows:
(1) water inlet 5 of Filter column 1 is connect by hose 9 with peristaltic pump, and water outlet 6 receives container with seawater and connect；
(2) peristaltic pump automatically twitches seawater through hose 9, and seawater enters branch from the water inlet 5 on Filter column 1 then
In space inside dagger 3, then through the collection environment DNA of filter membrane 4 built in support column 3, filtered seawater enters through air vent 7
Space between support column 3 and Filter column 1, and then seawater is drained by water outlet 6 and receives container 10；
(3) after seawater filters, using the liquid reliefs tool such as syringe or pipettor, DNA preservation solution is injected and is filtered
In column 1, so that high quality Conservation environment DNA sample, it is spare can be also placed in -20 DEG C of freezen protectives.
Claims (4)
1. a kind of device for collecting environment DNA in seawater, it is characterised in that: including Filter column (1), support column (3) and filter membrane
(4), support column (3) wherein are installed in Filter column (1), are divided into two skies by the support column (3) inside the Filter column (1)
Between, the internal space of the support column (3) is equipped with filter membrane (4), offers multiple air vents (7) on the support column (3)；It is described
One end of Filter column (1) offers the water inlet (5) being connected with support column (3) inner space, which passes through hose
(9) it is connected with peristaltic pump, the other end of the Filter column (1) offers the space phase between support column (3) and Filter column (1)
The water outlet (6) of connection, the water outlet (6) are received with seawater and are connected to inside container (10)；The peristaltic pump will be extra large through hose (9)
Water is evacuated in the internal space of support column (3), environment DNA is collected by filtration by the filter membrane (4), described in filtered seawater warp
Air vent (7) enters the space between the support column (3) and Filter column (1), and then drains into sea by the water outlet (6)
Water receives container (10).
2. collecting the device of environment DNA in seawater according to claim 1, it is characterised in that: the filter membrane (4) and support column
(3) inner wall abuts.
3. collecting the device of environment DNA in seawater according to claim 1, it is characterised in that: the Filter column (1) is divided into that
The upper cover (8) and cylinder (2) that this sealing is threadedly coupled, one end of the support column (3) removably inner surface with upper cover (8)
Connection.
4. collecting the device of environment DNA in seawater according to claim 1, it is characterised in that: the water inlet (5), water outlet
The longitudinal center line of mouth (6), Filter column (1) and support column (3) is conllinear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920125138.2U CN209619353U (en) | 2019-01-24 | 2019-01-24 | The device of environment DNA in a kind of collection seawater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920125138.2U CN209619353U (en) | 2019-01-24 | 2019-01-24 | The device of environment DNA in a kind of collection seawater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209619353U true CN209619353U (en) | 2019-11-12 |
Family
ID=68451514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920125138.2U Active CN209619353U (en) | 2019-01-24 | 2019-01-24 | The device of environment DNA in a kind of collection seawater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209619353U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607505A (en) * | 2020-06-04 | 2020-09-01 | 华南农业大学 | Water sample collecting and filtering device applied to estuary environment DNA research |
| CN112146932A (en) * | 2020-09-07 | 2020-12-29 | 陈琛 | A standardized quick acquisition equipment for extracting water body environment DNA |
-
2019
- 2019-01-24 CN CN201920125138.2U patent/CN209619353U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111607505A (en) * | 2020-06-04 | 2020-09-01 | 华南农业大学 | Water sample collecting and filtering device applied to estuary environment DNA research |
| CN111607505B (en) * | 2020-06-04 | 2021-09-21 | 华南农业大学 | Water sample collecting and filtering device applied to estuary environment DNA research |
| CN112146932A (en) * | 2020-09-07 | 2020-12-29 | 陈琛 | A standardized quick acquisition equipment for extracting water body environment DNA |
| CN112146932B (en) * | 2020-09-07 | 2023-07-21 | 陈琛 | Standardized rapid acquisition equipment for extracting water body environment DNA |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209619353U (en) | The device of environment DNA in a kind of collection seawater | |
| CN205038071U (en) | Water sample, dual -purpose collector of bed mud | |
| CN109536376B (en) | Water microorganism in-situ collection device | |
| CN112845522A (en) | Integrated device for efficiently separating micro-plastics in sediments and partially desorbing adsorbed pollutants and using method thereof | |
| CN202648987U (en) | Collecting device for suspended solids in water | |
| CN104458341A (en) | Portable box-type solid-phase extraction device for sampling natural water and natural water sampling method of portable box-type solid-phase extraction device | |
| CN106872225A (en) | A kind of water sample pretreatment device and its application method | |
| CN106769217B (en) | Karst mountain area soil solution exempts from to filter sampling and ion activity save set | |
| CN204182492U (en) | A kind of centrifugal filtration tube | |
| CN210180739U (en) | In-situ collection and filtration device based on shallow groundwater | |
| CN206219298U (en) | A kind of aquaculture wastewater biofilter | |
| CN213749214U (en) | Portable water sample drawing device | |
| CN204575391U (en) | A kind of solid source of release discharge the gas extractor of gas | |
| CN211318180U (en) | Pollution source waste gas monitoring device | |
| CN209148364U (en) | A kind of wild water sample collection filter device | |
| CN207163752U (en) | A kind of biological activated carbon pond water sampler | |
| CN111454937A (en) | Application of filter column in collection of environmental DNA in seawater | |
| CN107083324B (en) | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water | |
| CN203400567U (en) | Device for absorbing suspended matters on sewage | |
| CN208076204U (en) | Water-quality sampler | |
| CN216669420U (en) | External environment water sample collection system | |
| CN205903671U (en) | Aquaculture water quality testing sample water filter equipment | |
| CN213813004U (en) | Rapid filtering device for micro-plastic in water sample | |
| CN219890801U (en) | Water quality sampler filtering structure | |
| CN205280462U (en) | On --spot filter equipment of water sample particle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |