CN107083324B - Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water - Google Patents
Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water Download PDFInfo
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- CN107083324B CN107083324B CN201710244026.4A CN201710244026A CN107083324B CN 107083324 B CN107083324 B CN 107083324B CN 201710244026 A CN201710244026 A CN 201710244026A CN 107083324 B CN107083324 B CN 107083324B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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Abstract
The invention discloses a portable rapid bacterium collecting system for rapidly detecting microorganisms filled in drinking water. The invention is convenient to carry, can directly sample and collect bacteria in situ in a water plant to be detected, is not limited by using environmental conditions, and can effectively shorten the detection period; the invention is a closed structure, can effectively prevent from being polluted, and effectively ensure the accuracy of the bacteria collecting process; according to the invention, water samples can be pumped into the first bacteria collector and the second bacteria collector according to the culture requirements of different bacteria, so that the defect that multiple sampling is required in the traditional detection process is overcome, and the classification culture of different strains at the later stage is facilitated; the invention can extract the filling water sample, and has the advantages of convenient operation, high bacteria collecting efficiency and greatly reduced labor intensity; the outer side of the invention can also be used for mounting common detection equipment, and the invention has the advantage of wide application range.
Description
Technical Field
The invention belongs to the technical field of food microorganism detection, and particularly relates to a portable rapid bacterium collecting system for rapid detection of microorganisms in filled drinking water.
Background
At present, filling water is increasingly becoming the main drinking water source of common people, the market demand is increasing, and manufacturers are increasing, but the quality problem of the filling water that accompanies, especially the sanitary safety problem of water bottles and buckets used by the filling water becomes the focus of social attention. Food-borne biological pollution is one of the important factors of irrigation water pollution, and mainly comprises pathogenic bacteria such as total bacterial count, coliform group bacteria, mould yeast, pseudomonas aeruginosa and the like. According to documents and reports, a large amount of unqualified barreled water exists in a plurality of provinces and cities, and the filling water quality detection and monitoring responsibility is greater than that in mountains.
The contamination of filling water can be caused by several factors, such as contamination of water source, or stricter hygiene control in the production process, direct contact of non-sterilized hands of workers with mineral water or inner wall of container, or non-thorough cleaning and sterilizing of packaging container, which can cause the exceeding of microorganism in filling water.
Generally, in the process of the filling water spot check, firstly, a worker is required to go to a water plant to be detected for sampling, a sample filling water bucket is transported back to a laboratory for bacteria collection, cultivation and detection, the whole detection period generally needs 15-20 days, and the detection period is long. Although some novel microorganism quick detection means have appeared at present, the main existence of the method is limited by the use environment conditions, and related work can only be completed in a laboratory. Generally, when the filling water reaches a laboratory and is collected, the mold yeast and the pseudomonas aeruginosa are collected by a membrane filtration method specified by a standard, while the coliform group is directly cultured by pumping water, and for the conventional spot check equipment, the defect that repeated sampling is needed exists, the sampling efficiency is very low, particularly for common 18.9L barreled water, the target of spot check is usually 5 barrels of water in each group, and at this time, the water needs to be manually poured into different filter membranes to filter and collect the bacteria, so that the operation is inconvenient and the labor intensity is high; however, the existing sampling and detecting instruments are all open, and have the problem of easy pollution, thereby generating a false positive test result. Moreover, the traditional sampling inspection equipment needs to burn the filter disc by flame after filtering one filter cup, so that the accuracy of the experiment is not easy to guarantee while time is consumed, and in addition, the high-temperature open fire in an ultra-clean workbench or a biological safety cabinet has great potential safety hazard.
Therefore, the research and development of a portable bacteria collecting system which can directly collect bacteria in a sample taking way, shortens the detection period, ensures the accuracy of sampling, prevents a water sample from being polluted in the bacteria collecting process and is high in bacteria collecting efficiency is the key for solving the problems.
Disclosure of Invention
The invention aims to provide a portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water.
The invention aims to realize the purpose, which comprises an operation box, a box door, five bacteria collecting devices, an ultraviolet lamp and a power box, the box door is arranged on the side surface of the operation box, the front surface of the operation box is provided with an observation window for checking the operation inside the operation box, the front of the operation box is provided with an operation opening, the operation opening is provided with a glove, the edge of the opening of the glove is hermetically connected with the edge of the operation opening, the bacteria collecting device is arranged in the operation box and comprises a first bacteria collector, a second bacteria collector, a water pumping hose and a water pumping needle head, the top of the first bacteria collector is connected with a water pumping hose, the water inlet end of the water pumping hose is connected with a water pumping needle head, the second bacteria collector is connected with the upper part of the water pumping hose through a hose, the ultraviolet lamp is arranged at the top in the operation box, and the power supply box is arranged on the outer side of the operation box.
The invention has the beneficial effects that: the invention is convenient to carry, can directly sample and collect bacteria in the water plant to be detected, is not limited by the use environment condition, and can effectively shorten the detection period; the invention is a closed structure, can effectively prevent from being polluted, and effectively ensure the accuracy of the bacteria collecting process; according to the invention, water samples can be pumped into the first bacteria collector and the second bacteria collector according to the culture requirements of different bacteria, so that the defect that multiple sampling is required in the traditional detection process is overcome, and the classification culture of different strains at the later stage is facilitated; the invention can extract the filling water sample, and has the advantages of convenient operation, high bacteria collecting efficiency and greatly reduced labor intensity; the outer side of the invention can also be used for mounting common detection equipment, and the invention has the advantage of wide application range.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of a single bacteria collecting device;
FIG. 3 is a schematic view of the working state of the bacteria collector during water sampling;
FIG. 4 is a schematic top view of the bucket seat;
fig. 5 is a schematic view of a top view structure of the placing rack:
in the figure: 1-operation box, 2-box door, 3 a-sampling barrel, 3 b-barrel seat, 3 c-drainage nozzle, 3 d-bacteria-collecting filter membrane, 4 a-needle cylinder, 4 b-piston push rod, 4 c-water outlet nozzle, 4 d-water inlet nozzle, 4 e-air vent nozzle, 5-water-pumping hose, 6-water-pumping needle head, 7-peristaltic pump, 8-ultraviolet lamp, 9-observation window, 10-operation opening, 11-label plate, 12 a-fixed wafer, 12 b-rubber envelope, 12 c-air-permeable bolt, 13-placing frame, 13 a-support, 13 b-placing plate, 13 c-connecting upright rod, 13 d-ferrule, 14-water-draining hose, 15-first hanging piece, 16-second hanging piece and 17-protective cap, 18-handle, 19-plugging cap, 20-filling water and 21-pipe clamp.
Detailed Description
The invention is further described with reference to the accompanying drawings, but the invention is not limited in any way, and any alterations or substitutions based on the teaching of the invention are within the scope of the invention.
As shown in attached figures 1-5, the invention comprises an operation box 1, a box door 2, five bacteria collecting devices, an ultraviolet lamp 8 and a power supply box, wherein the box door 2 is arranged on the side surface of the operation box 1, the front surface of the operation box 1 is provided with an observation window 9 for checking the operation inside the operation box 1, the front surface of the operation box 1 is provided with an operation opening 10, a glove is arranged on the operation opening 10, the edge of the glove opening is hermetically connected with the edge of the operation opening 10, the bacteria collecting devices are arranged in the operation box 1 and comprise a first bacteria collector, a second bacteria collector, a water pumping hose 5 and a water pumping needle 6, the top of the first bacteria collector is connected with the water pumping hose 5, the water inlet end of the water pumping hose 5 is connected with the water pumping needle 6, the second bacteria collector is connected with the upper part of the water pumping hose 5 through a hose, the ultraviolet lamp 8 is arranged at the top inside the operation box 1, the power box is arranged on the outer side of the operation box 1.
First album of fungus ware including sampling bucket 3a, bucket seat 3b, sampling bucket 3a and bucket seat 3b between through helicitic texture swing joint, sampling bucket 3 a's top be equipped with water inlet, exhaust hole, bucket seat 3 b's bottom be the indent structure, bucket seat 3 b's bottom center be equipped with water drainage nozzle 3c, bucket seat 3b in be equipped with collection bacterial filter membrane 3d, the play water one end of water hose 5 be connected with sampling bucket 3 a's water inlet, water drainage nozzle 3c be equipped with the jam cap, the bottom in exhaust hole of sampling bucket 3a be equipped with air filter membrane.
The second bacteria collector comprises a needle cylinder 4a and a piston push rod 4b, a water outlet nozzle 4c is arranged at the bottom of the needle cylinder 4a, one part of the piston push rod 4b is arranged in the needle cylinder 4a, a water inlet nozzle 4d is arranged on the side surface of the middle part of the needle cylinder 4a, a vent nozzle 4e is arranged on the other side of the needle cylinder 4a opposite to the water inlet nozzle 4d, and the water inlet nozzle 4d is connected with the upper part of a water pumping hose 5 through a hose.
The body cover of the end of intaking of pumping hose 5 be equipped with closing device.
The sealing device comprises a fixed wafer 12a and a rubber sleeve 12b, wherein the fixed wafer 12a is sleeved on a pipe body at the water inlet end of the water pumping hose 5, the rubber sleeve 12b is fixedly connected with the edge of the fixed wafer 12a, and the fixed wafer 12a is provided with a ventilation bolt 12 c.
The sealing device comprises a fixed wafer 12a and a self-adhesive ring piece, wherein the fixed wafer 12a is sleeved on a pipe body at the water inlet end of the water pumping hose 5, the self-adhesive ring piece is arranged on the bottom surface of the fixed wafer 12a, and a ventilation bolt 12c is arranged on the fixed wafer 12 a.
Operation box 1 in be equipped with rack 13, first album of fungus ware, second album of fungus ware all locate on rack 13.
The first bacteria collector is placed in the placing hole of the placing plate 13 b.
The second bacteria collector is placed on the ferrule 13 d.
When the water pumping hose 5 works, the tube body of the water pumping hose 5 is connected with the peristaltic pump 7.
The side of the operation box 1 is provided with a first hanging part 15 and a second hanging part 16, the first hanging part 15 is hung with a solid alcohol lamp, and the second hanging part 16 is hung with a miniature incubator.
The operating box 1 is provided with a handle 18.
The bottom surface in the operation box 1 is provided with a pipe clamp placing groove, and a pipe clamp 21 is placed in the pipe clamp placing groove.
The wall of the sampling barrel 3a is provided with 50ml, 100ml, 150ml, 200ml, 250ml and 300ml volume scales.
The cylinder wall of the needle cylinder 4a is provided with 0.5ml, 1ml, 2ml, 3ml, 4ml and 5ml volume scales.
The length of the water pumping hose 5 is 140-200 cm.
The length of the water pumping needle head 6 is 15-20 cm.
The water pumping needle 6 is sleeved with a protective cap 17.
The water pumping hose 5 is provided with a fixing clamp.
The observation window 9 is a glass window.
The plugging cap is made of rubber.
The gloves are waterproof gloves.
The water outlet is provided with a water outlet hose 14.
One side of the placing plate 13b is provided with 5 label plates 11, and each label plate 11 corresponds to one bacteria collecting device.
The first bacteria collector and the second bacteria collector are both disposable sealed packages before use, and the sealed packages are removed during use.
The first bacteria collector and the second bacteria collector can be sterilized and reused.
The side surface of the operation box 1 is provided with a third hanging part 16, and the third hanging part 7 is used for hanging the peristaltic pump.
The working principle and the working process of the invention are as follows: firstly, taking the placing frame out of the operation box 1, then wiping a water gap of 20 filling water by using an alcohol cotton ball, and igniting an alcohol lamp near the water pumping needle head 6; taking down the protective cap 17, inserting the water pumping needle 6 into the filling water 20 from the water gap of the filling water 20 to be detected, turning down the rubber sleeve 12b, and sealing the water gap of the filling water 20 by the rubber sleeve 12b or adhering a non-drying adhesive ring piece to the water gap of the filling water 20; taking the placing frame 13 out of the operation box 1, removing the sealing package of the first bacteria collector and the second bacteria collector, then placing the first bacteria collector and the second bacteria collector into the placing frame 13, and connecting the water pumping hose 5; clamping a water pumping hose 5 at a water inlet of the first bacteria collector by using a pipe clamp 21, starting water inflow, enabling sample water to flow to a needle cylinder 4a of the second bacteria collector, and stopping water inflow when the sample water reaches a specified scale; then the pipe clamp 21 clamps the water pumping hose 5 at the water inlet nozzle 4d of the second bacteria collector, the sample water enters the sampling barrel 3a of the first bacteria collector, and when the water enters the specified scale, the water inlet is stopped; then the blocking cap of the drainage nozzle 3c of the barrel seat 3b is pulled out, and the blocking cap is plugged into the exhaust hole of the sampling barrel 3 a; then, starting to drain, wherein the sample water in the first bacteria collector leaks to the water containing tank from the water draining nozzle and is drained from the water draining port;
when the culture is needed, the first bacteria collector, the second bacteria collector and the placing frame are simultaneously placed into the operation box 1, an operator stretches the hand into the operation opening 10 to sleeve the glove, then unscrews the first bacteria collector, and takes out the bacteria-collecting filter membrane 3d and sends the bacteria-collecting filter membrane to a culture dish; taking down the second bacteria collector and aligning the second bacteria collector to the culture dish, pushing the piston push rod 4b, extruding the sample water in the needle cylinder 4a from the water outlet nozzle 4c into the culture dish, pouring the sample water into a culture medium, and culturing;
preferably, when the water pumping hose 5 works, the tube body of the water pumping hose 5 is connected with the peristaltic pump 7, and the peristaltic pump 7 pumps the water in the filling water 20.
The invention has the advantages that: the invention is convenient to carry, can directly sample and collect bacteria in the water plant to be detected, is not limited by the use environment condition, and can effectively shorten the detection period; the invention is a closed structure, can effectively prevent from being polluted, and effectively ensure the accuracy of the bacteria collecting process; according to the invention, water samples can be pumped into the first bacteria collector and the second bacteria collector according to the culture requirements of different bacteria, so that the defect that multiple sampling is required in the traditional detection process is overcome, and the classification culture of different strains at the later stage is facilitated; the invention can extract the filling water sample, and has the advantages of convenient operation, high bacteria collecting efficiency and greatly reduced labor intensity; the outer side of the invention can also be used for mounting common detection equipment, and the invention has the advantage of wide application range.
Claims (8)
1. A portable bacteria collection system for filling drinking water microorganism quick detection is characterized by comprising an operation box (1), a box door (2), five bacteria collection devices, an ultraviolet lamp (8) and a power supply box, wherein the box door (2) is arranged on the side face of the operation box (1), an observation window (9) for checking the internal operation of the operation box (1) is arranged on the front face of the operation box (1), an operation opening (10) is arranged on the front face of the operation box (1), a glove is arranged on the operation opening (10), and the edge of the glove opening of the glove is connected with the edge of the operation opening (10) in a sealing manner; the bacteria collecting device is arranged in the operation box (1), the bacteria collecting device comprises a first bacteria collector, a second bacteria collector, a water pumping hose (5) and a water pumping needle head (6), the top of the first bacteria collector is connected with a water pumping hose (5), the first bacteria collector comprises a sampling barrel (3 a) and a barrel seat (3 b), the sampling barrel (3 a) is movably connected with the barrel seat (3 b) through a thread structure, the top of the sampling barrel (3 a) is provided with a water inlet and an exhaust hole, the bottom of the barrel seat (3 b) is of a concave structure, a drainage nozzle (3 c) is arranged at the center of the bottom of the barrel seat (3 b), a bacteria-collecting filter membrane (3 d) is arranged in the barrel seat (3 b), one water outlet end of the water pumping hose (5) is connected with the water inlet of the sampling barrel (3 a), the drainage nozzle (3 c) is provided with a plugging cap, and the bottom of the exhaust hole of the sampling barrel (3 a) is provided with an air filter membrane; the second bacteria collector comprises a needle cylinder (4 a) and a piston push rod (4 b), a water outlet nozzle (4 c) is arranged at the bottom of the needle cylinder (4 a), one part of the piston push rod (4 b) is arranged in the needle cylinder (4 a), a water inlet nozzle (4 d) is arranged on the side surface of the middle part of the needle cylinder (4 a), a vent nozzle (4 e) is arranged on the other side, opposite to the water inlet nozzle (4 d), of the needle cylinder (4 a), and the water inlet nozzle (4 d) is connected with the upper part of a water pumping hose (5) through a hose; the water inlet end of the water pumping hose (5) is connected with a water pumping needle head (6); the ultraviolet lamp (8) is arranged at the top in the operation box (1), and the power supply box is arranged on the outer side of the operation box (1).
2. The portable bacteria collection system for the rapid microbial detection of filled drinking water as claimed in claim 1, wherein: the body cover of the end of intaking of pumping hose (5) is equipped with closing device.
3. The portable bacteria collection system for the rapid microbial detection of the filled drinking water as claimed in claim 2, wherein: the sealing device comprises a fixed wafer (12 a) and a rubber sleeve (12 b), the fixed wafer (12 a) is sleeved on a pipe body of a water inlet end of the water pumping hose (5), the rubber sleeve (12 b) is fixedly connected with the edge of the fixed wafer (12 a), and a ventilation bolt (12 c) is arranged on the fixed wafer (12 a).
4. The portable bacteria collection system for the rapid microbial detection of the filled drinking water as claimed in claim 2, wherein: the sealing device comprises a fixed wafer (12 a) and a self-adhesive ring piece, the fixed wafer (12 a) is sleeved on a pipe body at the water inlet end of the water pumping hose (5), the self-adhesive ring piece is arranged on the bottom surface of the fixed wafer (12 a), and a ventilation bolt (12 c) is arranged on the fixed wafer (12 a).
5. The portable bacteria collection system for the rapid microbial detection of filled drinking water as claimed in claim 1, wherein: be equipped with rack (13) in operation panel (1), first album of fungus ware, second album of fungus ware are all located on rack (13).
6. The portable bacteria collection system for the rapid microbial detection of the filled drinking water as claimed in claim 5, wherein: rack (13) include support (13 a), place board (13 b), connect pole setting (13 c), lasso (13 d), place board (13 b) and set up on support (13 a), place board (13 b) and be equipped with 5 and place the hole, and every right side of placing the hole all is equipped with and connects pole setting (13 c), connect pole setting (13 c) and place swing joint between board (13 b), lasso (13 d) set up in the top of connecting pole setting (13 c), and the below of placing board (13 b) is equipped with water containing tank, water containing tank's bottom is equipped with down the mouth of a river.
7. The portable bacteria collection system for the rapid microbial detection of filled drinking water as claimed in claim 1, wherein: when the water pumping hose (5) works, the tube body of the water pumping hose (5) is connected with the peristaltic pump (7).
8. The portable bacteria collection system for the rapid microbial detection of filled drinking water as claimed in claim 1, wherein: the side of the operation box (1) is provided with a first hanging part (15) and a second hanging part (16), the first hanging part (15) is provided with a solid alcohol lamp in a hanging mode, and the second hanging part (16) is provided with a miniature incubator in a hanging mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710244026.4A CN107083324B (en) | 2017-04-14 | 2017-04-14 | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710244026.4A CN107083324B (en) | 2017-04-14 | 2017-04-14 | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
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| Publication Number | Publication Date |
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| CN107083324A CN107083324A (en) | 2017-08-22 |
| CN107083324B true CN107083324B (en) | 2020-03-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710244026.4A Active CN107083324B (en) | 2017-04-14 | 2017-04-14 | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111808734A (en) * | 2020-07-01 | 2020-10-23 | 深圳市核子基因科技有限公司 | Bacterial colony sampling device for biotechnology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003072807A2 (en) * | 2002-02-28 | 2003-09-04 | Houghton Associates | Sterility testing apparatus |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2147894Y (en) * | 1992-11-19 | 1993-12-01 | 倪卫菊 | Disposable millipore filter |
| CN2332725Y (en) * | 1996-11-04 | 1999-08-11 | 叶大林 | Bacteria gathering culture dish |
| CN202061628U (en) * | 2011-04-18 | 2011-12-07 | 浙江工业职业技术学院 | Glove box type biosafety cabinet |
| CN202430211U (en) * | 2011-12-28 | 2012-09-12 | 芜湖先声中人药业有限公司 | Culture box support applicable to storing bacterium collectors |
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- 2017-04-14 CN CN201710244026.4A patent/CN107083324B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003072807A2 (en) * | 2002-02-28 | 2003-09-04 | Houghton Associates | Sterility testing apparatus |
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