CN209226998U - A kind of visible multichannel hydrodynamic shear cell culture apparatus - Google Patents
A kind of visible multichannel hydrodynamic shear cell culture apparatus Download PDFInfo
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- CN209226998U CN209226998U CN201820931707.8U CN201820931707U CN209226998U CN 209226998 U CN209226998 U CN 209226998U CN 201820931707 U CN201820931707 U CN 201820931707U CN 209226998 U CN209226998 U CN 209226998U
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Abstract
The utility model belongs to field of biomedicine, disclose a kind of visible multichannel hydrodynamic shear cell culture apparatus, it provides a kind of realization and cultivates piece more and carry out the lower culture cell of hydrodynamic shear stimulation and do not open cavity when can test carrying out observation cell, it is and reusable, it is ensured that the cell culture apparatus and its method of engineering repeatability；By hydrodynamic shear cell culture chamber, circulatory system pressure adjuster, damper, pump, fluid reservoir and pipeline are formed, and are controlled by computer control system；It is overflow using on gravity liquid substantially in sustained height with the hydrodynamic shear cell culture chamber placed vertically, hydrodynamic shear size engineering repeatability is realized in the way of the feed flow of center simultaneously, and can realize micro- sem observation, facilitate the research of external hydrodynamic shear cell culture experiments.
Description
Technical field
The utility model belongs to biomedical engineering field, discloses a kind of visible multichannel hydrodynamic shear cell culture
Device provides a kind of simulate in vitro and carries out hydrodynamic shear to culture on piece cell under Human Physiology or non-physiological environment
The lower experimental provision cultivated of stimulation.
Background technique
The cell of hydrodynamic shear stimulation at present, which carries out dynamic cultivation, many methods, but often monolithic culture piece
Cell culture amount not enough or hydrodynamic shear load precision it is inadequate.
When mostly culture piece is cultivated simultaneously, and it is difficult under the premise of not opening culture chamber to cellular morphology, state
It is observed.
Utility model content
Solve the above problems to realize, we illustrate with can multi-disc culture piece cultivated simultaneously, put vertically
It sets, with form observation, by the fluid circulation culture apparatus of the hydrodynamic shear cell culture chamber of center feed flow.
To achieve the goals above, a kind of the technical solution adopted in the utility model are as follows: visible multichannel hydrodynamic shear
Cell culture apparatus, it includes the multi-channel fluid shearing force cell culture chambers for having form, and access adjustable pressure and become
Change the fluid circulating system with changes in flow rate；The fluid circulating system of the adjustable pressure change and changes in flow rate is in addition to institute
It states outside cell culture chamber, also includes the circulatory system pressure adjuster and reality for adjusting pressure change in fluid circulating system
The pump of flow supply variation, cooperates the circulatory system pressure adjuster to realize pressure controlled damping in existing fluid circulating system
The pipeline of the fluid reservoir and the entire loop of connection of adjuster and liquid storage, passes through the pipeline out of described fluid reservoir by the pump
It draws liquid and flows through the training that the cell culture chamber is applied in the culture chamber then to the fluid circulating system feed flow
The cell flow shearing force of on piece is supported, finally in the liquid reflux to fluid reservoir in the fluid circulating system；By calculating
Machine control system is realized to the acquisition of pressure sensor data and to the control of the pump, the circulatory system pressure adjuster
Function is stated, and is placed on CO2In incubator or enable culture solution the environment for possessing thermostat and 5% carbon dioxide it
In cultivated.
Further, the pump, which can according to need, controls the control that the flow in the fluid circulating system temporally changes
System, forms the supply of various flow rate modes, flow supply model includes steady state value flow, pulse in fluid circulating system loop
Formula flow, sine wave flow, cardiac pumping formula flow and a variety of different flow rate modes, and then realize intracavitary to cell culture
Culture piece applies lower than physiology flow to the stimulation for being higher than the formation of physiology flow, while pump connects with the liquid in fluid circulating system
Contact portion point has dedicated elastomer isolators, realizes that the liquid in fluid circulating system will not be by except the separator of pump and pump
Part is in contact, and the elastomer isolators will not be formed with other solid members with liquid contact portion in fluid circulating system and be contacted
Movement, the elastomer isolators material can be handled with resistance to medical autoclave sterilization;The range of flow 0-2000mL/min of pump.
Further, the circulatory system pressure adjuster is by pressure chamber, pressure space magnitude regulator, pressure sensing
Device, barostat composition；The pressure sensor can carry out data acquisition and pressure space by the computer control system
Magnitude regulator, barostat are controlled to adjust by computer control system, can also be adjusted by manually carrying out pressure adjusting
Mode, which can be, changes pressure chamber's space size, be also possible to change pressure gas pressure inside size or two kinds simultaneously into
Row is adjusted, and the indoor culture solution liquid level of pressure is higher than culture solution and passes in and out open height, and the circulatory system pressure adjuster is realized
Pressure regulation spectrum is in 0-300mmHg.
Further, the multi-channel fluid shearing force cell culture chamber with form is by main body, peripheral element, up and down
Sealing element, upper cover, lower cover, connector, supporting block composition;The main body is to produce flowing groove and training for laminar flow on peripheral surface
Piece placement space is supported, described 5 millimeters to 30 millimeters of flowing groove width, the height 0.05 of flowing groove is between 1mm；The main body is perpendicular
Straight to place, using principle is overflow on liquid, close to unanimously, Xiang Liudong tank culture piece applies fluid and cuts the same height liquid level rate of climb
Shear force, main body have peripheral element encirclement, are sealed up and down by the sealing element, and when upper and lower covers squeeze sealing member, sealing element is simultaneously to training
It supports piece and forms extruding, so that the culture piece is bonded completely with the flow path groove of the laminar flow in main body one side, so that hydrodynamic shear
The repeatability of size is higher；It is connected to connector at the center of the upper and lower covers simultaneously, accesses fluid circulating system, is cooperated described above
Liquid on overflow it is original so that the hydrodynamic shear that the liquid flowed into from interface is formed is in cell culture on piece function and effect phase
Together；The peripheral element is supported by surrounding body, form, form forms, and has form in the peripheral element, form is fixed on form
In support, so that flow cavity is in use, flowing cavity can not be opened, micro- sem observation culture on piece can be used
Cellular morphology；Entire cell culture chamber can carry out autoclave sterilization, the culture piece quantity 1-12 for the culture that can be carried out simultaneously
Piece.
Further, the culture piece thickness cultivates the dimensions length of piece: 18 to 100 millimeters, wide between 0.1-1mm
18 to 40 millimeters of degree, aspect ratio range: 1 to 4 times；Culture piece placement space height according to culture piece thickness make, and
The height of slightly higher degree culture piece, is no more than 10%；The material of the culture piece is category of glass or transparent non-metallic material.
Further, the variation of circulation line latus rectum may be implemented in the damper regulator, simulates the effect of capillary,
And it may be implemented to finely tune, to cooperate the variation for realizing pressure in circulatory system pressure adjuster regulating system.
Further, the effect of liquid storage may be implemented in the fluid reservoir, for liquid reflux storage and dosing etc..
The utility model additionally provides the application method of visible multichannel hydrodynamic shear cell culture apparatus, including as follows
Step:
(1) the multi-channel fluid shearing force cell culture chamber to described with form, the fluid circulating system, described follow
Loop system pressure regulator, the part contacted with culture solution carry out sterilization；
(2) experiment culture piece is got out；
(3) all component parts of the visible multichannel hydrodynamic shear cell culture apparatus are placed in Biohazard Safety Equipment
It is inside assembled and is connected, form loop, connect blood vessel distortion adjuster, detecting the whole device after the completion of connection ensures
Culture solution does not leak；
(4) start fluid circulating system, observe pressure sensor value, adjust circulatory system pressure adjuster, fluid is followed
The pressure value of loop system is adjusted to target pressure value, sets hydrodynamic shear target value, setup parameter；
(5) runing time is set, starts the visible multichannel hydrodynamic shear cell culture and holds device, can carry out
Hydrodynamic shear cell culture;
(6) in incubation, pump can be suspended, stop hydrodynamic shear load, then carry out micro- sem observation, using
Cell state is checked in the process.
In conclusion by adopting the above-described technical solution, the utility model has the following beneficial effects:
1. the utility model realizes the hydrodynamic shear device that can cultivate volume cell；
The experimental provision of visual window observation cell when 2. the utility model realizes experiment；
3. the utility model realize simulation physiological status under or non-physiological state under hydrodynamic shear cell culture fill
It sets；
4. the utility model improves the engineering repeatability of hydrodynamic shear and itself precision of hydrodynamic shear load；
Therefore the utility model provides a kind of visible multichannel hydrodynamic shear cell culture apparatus.
Detailed description of the invention
Fig. 1 is the system diagram of the utility model embodiment；
Fig. 2 is the visual hydrodynamic shear culture chamber structure chart of multichannel in the utility model embodiment；
Fig. 3 is the visual hydrodynamic shear culture chamber cross-sectional view 1 of multichannel in the utility model embodiment；
Fig. 4 is the visual hydrodynamic shear culture chamber cross-sectional view 2 of multichannel in the utility model embodiment；
Fig. 5 is circulatory system pressure adjuster figure in the utility model embodiment；
Fig. 6 is the schematic diagram pumped in the utility model embodiment；
In figure, 1- visible multichannel hydrodynamic shear cell culture chamber, 1-1- main body, the periphery 1-2-, the periphery 1-2-1 master
Body, the support of 1-2-2- form, 1-2-3- form, 1-3- sealing element, 1-4- upper cover, 1-5- lower cover, 1-6- connector, 1-7- support
Block, 1-8 sealing element；2- circulatory system pressure adjuster, the pressure chamber 2-1-, the pressure chamber 2-2- space regulator, 2-3- pressure pass
Sensor, 2-4- barostat, 2-5 circulation fluid import, the outlet of 2-6 circulation fluid；3-CO2Incubator, 4- pump, the outlet 4-1- are single
To valve, 4-2- import check valve, 4-3- pump chamber, 4-4- elastomeric isolation film, 4-5- plunger stem, 4-6- sealing element, 5- damping is adjusted
Save device, 6- fluid reservoir, 7- computer control system, 8- pipeline；
A- microscope, B- fluidized bed chute, C- cultivate piece, and D- cultivates piece placement space, and E- pumps interior fluid circulating system training
Nutrient solution, F- pump driving liquid.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
It should be noted that it can be directly on another component when component is referred to as " being fixed on " another component
Or there may also be components placed in the middle；When a component is considered as " connection " another component, it, which can be, is directly connected to
To another component or it may be simultaneously present component placed in the middle；Term as used herein "upper", "lower", "left", "right" and
Similar statement is for illustrative purposes only.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical；Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term " and or " used herein includes
Any and all combinations of one or more related listed items.
Embodiment
A kind of visible multichannel hydrodynamic shear cell culture apparatus is thin comprising the multi-channel fluid shearing force with form
Born of the same parents' culture chamber (1), and access the fluid circulating system of adjustable pressure change and changes in flow rate；The adjustable pressure becomes
Change and the fluid circulating system of changes in flow rate is other than the cell culture chamber (1), also includes for adjusting fluid circulation system
The circulatory system pressure adjuster (2) for interior pressure change of uniting and the pump (4) for realizing flow supply variation in fluid circulating system, match
The circulatory system pressure adjuster (2) is closed to realize the fluid reservoir (6) of pressure controlled damper regulator (5) and liquid storage and connect
The pipeline (8) for connecing entire loop draws liquid by the pipeline (8) by pumping (4), then to institute out of the fluid reservoir (6)
Fluid circulating system feed flow is stated, is flowed through on the culture piece (C) that the cell culture chamber (1) is applied in cell culture chamber (1)
Cell flow shearing force, finally in the liquid reflux to fluid reservoir in the fluid circulating system；It is controlled by computer and is
Above-mentioned function is realized in acquisition of the system to pressure sensor (2-3) data and the control to pump (4), circulatory system pressure adjuster (2)
Can, and it is placed on CO2In incubator (3) or enable culture solution among the environment for possessing thermostat and 5% carbon dioxide
It is cultivated.
Further, pump (4) is by import check valve (4-2), Outlet check valves (4-1), the pump housing (4-3), isolation film (4-
4), plunger stem (4-5), sealing element (4-6) composition；Due to the culture of check valve, when plunger stem (4-5) is far from isolation film
(4-4) inlet side can be to fluid reservoir (6) imbibition, can be to fluid circulation system when plunger stem (4-5) is close to isolation film (1-4)
System conveying liquid；Therefore it can according to need the control of control plunger stem temporally changed on reciprocating direction, then it can be
The supply of various flow rate modes is formed in fluid circulating system loop, flow supply model includes steady state value flow, pulsed stream
Amount, sine wave flow, cardiac pumping formula flow and a variety of different flow rate modes, and then realize to the intracavitary culture piece of cell culture
On cell apply lower than physiology flow or higher than physiology flow formed stimulation；Pump and the liquid in fluid circulating system simultaneously
Contact portion has dedicated elastomer isolators, realizes that the liquid in fluid circulating system will not be by the separator of pump (4) and pump
Part except (4-4) is in contact；It will not be formed and be connect with other solid members with liquid contact portion in fluid circulating system simultaneously
Touching movement, elastomer isolators (4-4) material can be with the elastic materials of resistance to medical autoclave sterilization processing；Pump the flow model of (4)
Enclose 0-2000mL/min.
Further, circulatory system pressure adjuster (2) is by pressure chamber (2-1), pressure space magnitude regulator (2-
2), pressure sensor (2-3), barostat (2-4) composition；Pressure sensor (2-3) can by computer control system (7) into
The acquisition of row data and pressure space magnitude regulator (2-2), barostat (2-4) are controlled by computer control system (7)
System is adjusted, can also be by manually carrying out pressure adjusting, and regulative mode, which can be, changes pressure chamber's space size, is also possible to change pressure
Power gas pressure inside size or two kinds are adjusted simultaneously, and circulatory system pressure adjuster (2) realizes pressure regulation spectrum
In 0-300mmHg, the liquid level of the indoor culture solution of pressure is higher than culture solution and passes in and out open height.
Further, the multi-channel fluid shearing force cell culture chamber (1) with form is by main body (1-1), outside
It encloses part (1-2), upper-lower seal part (1-3), upper cover (1-4), lower cover (1-5), connector (1-6), supporting block (1-7) composition;Main body
(1-1) is to produce flowing groove (B) and culture piece placement space (D) for laminar flow, 5 milli of flowing groove width on peripheral surface
Rice is to 30 millimeters, and the height 0.05 of flowing groove (B) is between 1mm；The main body is placed vertically, using principle is overflow on liquid, together
For the sample height liquid level rate of climb close to unanimously, Xiang Liudong tank culture piece applies hydrodynamic shear, and main body (1-1) has peripheral element packet
It encloses, is sealed up and down by sealing element (1-3), when upper and lower covers (1-4), (1-5) squeeze sealing member (1-3), sealing element (1-3) is right simultaneously
It cultivates piece (C) and forms extruding, so that flow path groove (B) one side of culture piece (C) and the laminar flow on main body (1-1) is pasted completely
It closes so that the repeatability of hydrodynamic shear size is higher；It is connected to connector at the center of the upper and lower covers (1-4), (1-5) simultaneously,
Fluid circulating system is accessed, cooperates and is overflow originally on liquid recited above, so that the fluid that the liquid flowed into from interface is formed
Shearing force is identical in cell culture on piece function and effect；The peripheral element (2) by surrounding body (2-1), form support (2-2),
Form (2-3) forms, and has form (2-3) in peripheral element (2), and form (2-3) is fixed on form and supports on (2-2), so that
Flow cavity in use, can not open flowing cavity, can use the cellular morphology of micro- sem observation culture on piece；Entirely
Cell culture chamber can carry out autoclave sterilization, culture piece (C) the quantity 1-12 piece for the culture that can be carried out simultaneously.
Further, culture piece (1) thickness is between 0.1-1mm, cultivates the dimensions length of piece: 18 to 100 millimeters,
18 to 40 millimeters of width, aspect ratio range: 1 to 4 times；Culture piece placement space (D) height according to culture piece thickness system
Make, and the height of slightly higher degree culture piece, is no more than 10%；The material of the culture piece is category of glass or transparent non-metallic material.
Further, the variation of circulation line latus rectum may be implemented in damper regulator (5), simulates the effect of capillary,
And it may be implemented to finely tune, to cooperate the variation for realizing pressure in circulatory system pressure adjuster (2) regulating system.
Further, the effect of liquid storage may be implemented in fluid reservoir (6), for liquid reflux storage and dosing etc..
The utility model additionally provides the application method of visible multichannel hydrodynamic shear cell culture apparatus, including as follows
Step:
(1) the multi-channel fluid shearing force cell culture chamber to described with form, the fluid circulating system, described follow
Loop system pressure regulator, the part contacted with culture solution carry out sterilization；
(2) experiment culture piece is got out；
(3) all component parts of the visible multichannel hydrodynamic shear cell culture apparatus are placed in Biohazard Safety Equipment
It is inside assembled and is connected, form loop, connect blood vessel distortion adjuster, detecting the whole device after the completion of connection ensures
Culture solution does not leak；
(4) start fluid circulating system, observe pressure sensor value, adjust circulatory system pressure adjuster, fluid is followed
The pressure value of loop system is adjusted to target pressure value, sets hydrodynamic shear target value, setup parameter；
(5) runing time is set, starts the visible multichannel hydrodynamic shear cell culture and holds device, can carry out
Hydrodynamic shear cell culture；
(6) in incubation, pump can be suspended, stop hydrodynamic shear load, then carry out micro- sem observation, make
With checking cell state in the process.
Above description is the detailed description for the preferable possible embodiments of the utility model, but embodiment is not limited to
The patent claim of the utility model, the same changes or modifications completed under technical spirit suggested by all the utility model
Change, should belong to the covered the scope of the patents of the utility model.
Claims (6)
1. a kind of visible multichannel hydrodynamic shear cell culture apparatus, it is characterised in that the multi-channel fluid comprising having form
Shearing force cell culture chamber, and access the fluid circulating system of adjustable pressure change and changes in flow rate；It is described adjustable
The fluid circulating system of pressure change and changes in flow rate also includes for adjusting fluid circulation other than the cell culture chamber
The circulatory system pressure adjuster of system pressure variation and the pump for realizing flow supply variation in fluid circulating system, cooperate institute
Circulatory system pressure adjuster is stated to realize the fluid reservoir of pressure controlled damper regulator and liquid storage and connect entire loop
Pipeline draws liquid by the pipeline by the pump, then to the fluid circulating system feed flow, stream out of described fluid reservoir
The cell flow shearing force of the culture on piece in the culture chamber is applied to through the cell culture chamber, the last fluid
In liquid reflux to fluid reservoir in the circulatory system；By computer control system to the acquisition of pressure sensor data and to described
Above-mentioned function is realized in the control of pump, the circulatory system pressure adjuster, and is placed on CO2In incubator or enable culture solution
It is enough to be cultivated among the environment for possessing thermostat and 5% carbon dioxide.
2. a kind of visible multichannel hydrodynamic shear cell culture apparatus according to claim 1, it is characterised in that the pump
It can according to need and control the control that the flow in the fluid circulating system temporally changes, in fluid circulating system loop
The supply of various flow rate modes is formed, flow supply model includes steady state value flow, pulsed flow, sine wave flow, heart
Blood formula flow rate mode is pumped, and then realizes that the culture piece intracavitary to cell culture applies lower than physiology flow to being higher than physiology flow shape
At stimulation, while pumping and have a dedicated elastomer isolators with the liquid contact portion in fluid circulating system, realize fluid circulation
Liquid in system will not be in contact by pumping with the part except the separator of pump, the elastomer isolators and fluid circulation system
Liquid contact portion will not form contact movement with other solid members in uniting, and the elastomer isolators material can be with resistance to medical high temperature
High pressure sterilization processing;The range of flow 0-2000mL/min of pump.
3. a kind of visible multichannel hydrodynamic shear cell culture apparatus according to claim 1, it is characterised in that described to follow
Loop system pressure regulator is made of pressure chamber, pressure space magnitude regulator, pressure sensor, barostat；It is described
Pressure sensor can carry out data acquisition and pressure space magnitude regulator, barostat by the computer control system
It is controlled to adjust by computer control system, it can also be by manually carrying out pressure adjusting, regulative mode, which can be, changes pressure chamber
Space size is also possible to change pressure gas pressure inside size or two kinds while be adjusted, the indoor culture of pressure
The liquid level of liquid is higher than culture solution and passes in and out open height, and the circulatory system pressure adjuster realizes pressure regulation spectrum in 0-
300mmHg。
4. a kind of visible multichannel hydrodynamic shear cell culture apparatus according to claim 1, it is characterised in that described
Multi-channel fluid shearing force cell culture chamber with form is by main body, peripheral element, upper-lower seal part, upper cover, lower cover, connector,
Supporting block composition;The main body is to produce flowing groove and culture piece placement space for laminar flow, the flowing on peripheral surface
5 millimeters to 30 millimeters of groove width, the height 0.05 of flowing groove is between 1mm；The main body is placed vertically, former using overflowing on liquid
Reason, for the same height liquid level rate of climb close to unanimously, Xiang Liudong tank culture piece applies hydrodynamic shear, and main body has peripheral element packet
It encloses, is sealed up and down by the sealing element, when upper and lower covers squeeze sealing member, sealing element forms culture piece squeeze simultaneously, so that institute
It states culture piece to be bonded completely with the flow path groove of the laminar flow in main body one side so that the repeatability of hydrodynamic shear size is higher；Together
When at the center of the upper and lower covers be connected to connector, access fluid circulating system, cooperate overflow on liquid recited above it is original so that
The hydrodynamic shear that the liquid flowed into from interface is formed is identical in cell culture on piece function and effect；The peripheral element is by periphery
Main body, form are supported, form forms, and have form in the peripheral element, and form is fixed on form and supports, so that flowing
Chamber in use, can not open flowing cavity, can use the cellular morphology of micro- sem observation culture on piece；Entire cell
Culture chamber can carry out autoclave sterilization, the culture piece quantity 1-12 piece for the culture that can be carried out simultaneously；The culture piece thickness exists
Between 0.1-1mm, the dimensions length of piece is cultivated: 18 to 100 millimeters, 18 to 40 millimeters of width, aspect ratio range: 1 to 4 times；'s
The height for cultivating piece placement space makes according to the thickness of culture piece, and the height of slightly higher degree culture piece, is no more than 10%；It is described
The material for cultivating piece is category of glass or transparent non-metallic material.
5. a kind of visible multichannel hydrodynamic shear cell culture apparatus according to claim 1, it is characterised in that the resistance
The variation of circulation line latus rectum may be implemented in Buddhist nun's adjuster, simulates the effect of capillary, and may be implemented to finely tune, to match
Close the variation for realizing pressure in circulatory system pressure adjuster regulating system.
6. a kind of visible multichannel hydrodynamic shear cell culture apparatus according to claim 1, it is characterised in that the storage
The effect of liquid storage may be implemented in liquid chamber, for liquid reflux storage and dosing.
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| CN201820931707.8U CN209226998U (en) | 2018-06-15 | 2018-06-15 | A kind of visible multichannel hydrodynamic shear cell culture apparatus |
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| CN201820931707.8U CN209226998U (en) | 2018-06-15 | 2018-06-15 | A kind of visible multichannel hydrodynamic shear cell culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108467837A (en) * | 2018-06-15 | 2018-08-31 | 上海天引生物科技有限公司 | A kind of visible multichannel hydrodynamic shear cell culture apparatus and its method |
| CN114752494A (en) * | 2022-03-24 | 2022-07-15 | 四川大学 | Cell culture chamber, adjustable cell mechanical stimulation culture device and manufacturing method thereof |
-
2018
- 2018-06-15 CN CN201820931707.8U patent/CN209226998U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108467837A (en) * | 2018-06-15 | 2018-08-31 | 上海天引生物科技有限公司 | A kind of visible multichannel hydrodynamic shear cell culture apparatus and its method |
| CN108467837B (en) * | 2018-06-15 | 2023-08-18 | 上海天引生物科技有限公司 | Visual multichannel fluid shear force cell culture device and method thereof |
| CN114752494A (en) * | 2022-03-24 | 2022-07-15 | 四川大学 | Cell culture chamber, adjustable cell mechanical stimulation culture device and manufacturing method thereof |
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