CN102010821A - Cell gaseous exposure experiment control device - Google Patents
Cell gaseous exposure experiment control device Download PDFInfo
- Publication number
- CN102010821A CN102010821A CN2010102702439A CN201010270243A CN102010821A CN 102010821 A CN102010821 A CN 102010821A CN 2010102702439 A CN2010102702439 A CN 2010102702439A CN 201010270243 A CN201010270243 A CN 201010270243A CN 102010821 A CN102010821 A CN 102010821A
- Authority
- CN
- China
- Prior art keywords
- cell
- experiment
- gaseous state
- control device
- exposure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a cell gaseous exposure experiment control device which comprises a cell gaseous exposure experiment cavity and a cell culture solution cavity. A special microporous film plate for experiment cells is arranged between the two cavities, a cell culture solution supply device keeps the back surface of the microporous film plate to be constantly immersed in the cell culture solution so as to supply nutrients necessary for the growth of the experiment cells, and meanwhile, top surfaces of cells on the microporous film plate can be directly exposed in the cell gaseous exposure experiment cavity to receive the exposure contamination of a gaseous substance. A warm bath cavity tightly attached to the two cavities and controlled by a warm bath control device ensures that the experiment cells always grow normally under the temperature condition of 37 DEG C. A special gas generation control device special for experiments is connected with the cell gaseous exposure experiment cavity through a gas supply manifold to complete various cell gaseous exposure contamination experiments. The invention has the advantage of providing a new gaseous exposure contamination experiment method, is widely applied to the fields of public health environmentology, molecular biology, cytology, toxicology and the like and can be used for partially replacing traditional animal living bodies to carry out the experiments.
Description
Technical field
The present invention relates to a kind of cell gaseous state and expose experiement control device.
Background technology
In the scientific domains such as biology in the past, environmentalism, diseases prevention and control, medical science, military science, toxicology, toxic and harmful exposes the harm research that causes must carry out traditional animal gaseous state exposure exposure experiment.Present stage gaseous state exposure exposure experiment also rests on animal experiment stage; the laboratory animal quantity that needs is many, experiment condition is harsh, animal individual difference causes greatly that experiment effect is not good, the cost height of experiment and the requirement of animal welfare and protection aspect etc. are unfavorable for factor, is badly in need of the alternative experimental technique of one or more animals.Cell toxicology research has just in time remedied deficiency in this respect, and is still blank at present but the gaseous state that theory and practice all needs exposes experiement control device.The cell gaseous state exposes exposure experiment and only rests on theoretical investigation or only depend on manual experiment to finish, and seriously restricts application and the development of cell toxicology in this field scientific research.
Summary of the invention
For addressing the above problem, the invention provides a kind of cell gaseous state and expose experiement control device, utilize the experimental technique of this exploration of cell cultures and indicator cells vital movement rule, can guarantee accurately to control when safety is carried out cell cultures the concentration and the flow of experiment contamination gas.The direct gaseous state of realizing pair cell exposes exposure experiment, substitutes traditional experimentation on animals.
For achieving the above object, technical scheme of the present invention provides a kind of cell gaseous state and exposes experiement control device, comprises that the cell gaseous state exposes experiment chamber and cell cultures sap cavity.Place the micropore lamina membranacea of experimental cell special use between two cavitys, cell cultures liquid supply unit maintenance cell culture fluid soaks into micropore lamina membranacea bottom surface constantly and offers the required nutrition of experimental cell growth, and the cell upper surface on the micropore template can be directly exposed to the exposure contamination of accepting gaseous substance in the cell gaseous state exposure chamber again simultaneously.Be close to the temperature that has the temperature of being subjected to bathe control device control on two cavitys and bathe chamber assurance experimental cell normal growth under 37 ℃ temperature condition all the time.Experiment special gas generation control device links to each other to fetch by air feed branch pipe and cell gaseous state exposure experiment chamber and finishes various types of cells gaseous state exposure exposure experiment.
Effect of the present invention is: changed the present stage gaseous state and exposed the backward situation that exposure experiment also rests on animal experiment stage.Overcome that the laboratory animal quantity that needs is many, experiment condition is harsh, animal individual difference causes greatly that experiment effect is not good, the cost height of testing and the requirement of animal welfare and protection aspect etc. are unfavorable for factor.Adopt a kind of science and convenient effective cell gaseous state to expose experimental technique and substituted traditional animal experiment method.Filled up technological gap in this respect.Promoted application and the development of cell toxicology in this field scientific research.
Description of drawings
Accompanying drawing is the synoptic diagram of structure of the present invention.
Among the figure:
1, air feed branch pipe
2, the cell gaseous state exposes the experiment chamber
3, micropore lamina membranacea
4, cell cultures sap cavity
5, temperature is bathed the chamber
6, cell cultures liquid supply unit
7, nutrient solution flow director
8, temperature is bathed control device
9, experimental gas generating unit
10, gas exposure concentrations controller
11, gas flow controller
Embodiment
Reaching embodiment in conjunction with the accompanying drawings is illustrated the present invention.
As shown in drawings, its cell gaseous state is exposed the micropore lamina membranacea of placing the experimental cell special use between experiment chamber and cell cultures sap cavity two cavitys, cell cultures liquid supply unit maintenance cell culture fluid soaks into micropore lamina membranacea bottom surface constantly and offers the required nutrition of experimental cell growth, and the cell upper surface on the micropore template can be directly exposed to the exposure contamination of accepting gaseous substance in the cell gaseous state exposure chamber again simultaneously.Be close to the temperature that has the temperature of being subjected to bathe control device control on two cavitys and bathe chamber assurance experimental cell normal growth under 37 ℃ temperature condition all the time.Experiment special gas generation control device links to each other to fetch by air feed branch pipe and cell gaseous state exposure experiment chamber and finishes various types of cells gaseous state exposure exposure experiment.
When using, at first open the cell gaseous state and expose micropore template that the experiment chamber will have an experimental cell with cultivating and after being placed under the aseptic condition on the cell cultures sap cavity, steadily putting well, the cell gaseous state exposed and test the chamber and cover completely.Regulating the nutrient solution flow director makes nutrient solution just reach micropore template lower surface to have and can not flood the experimental cell upper surface.Open temperature and bathe control device, guarantee that temperature bath chamber guarantees experimental cell normal growth under 37 ℃ temperature condition all the time.Regulate consistency controller and flow director on the experimental gas generating unit then, open the experimental gas generating unit.Experimental gas is transported to the upper surface that spreads in experimental cell in the cellular exposure chamber uniformly experimental cell is carried out gaseous state exposure exposure experiment by the air feed branch pipe.After finishing, experiment closes experimental gas generating unit and nutrient solution flow director and temperature bath control device.Open the cell gaseous state and expose micropore template that the experiment chamber will have experimental cell is taken out the processes such as growth, breeding, cytodifferentiation and cell aging of carrying out observation of cell under aseptic condition biological phenomena and the detection of the correlated results aspect the biology.
Claims (5)
1. a cell gaseous state exposes experiement control device, it is characterized in that: the cell gaseous state exposes experiment chamber and cell cultures sap cavity, place the micropore lamina membranacea of experimental cell special use between two cavitys, cell cultures liquid supply unit maintenance cell culture fluid soaks into micropore lamina membranacea bottom surface constantly and offers the required nutrition of experimental cell growth, cell upper surface on the micropore template can be directly exposed to the exposure contamination of accepting gaseous substance in the cell gaseous state exposure chamber again simultaneously, be close to the temperature that has the temperature of being subjected to bathe control device control on two cavitys and bathe the chamber and guarantee experimental cell normal growth under 37 ℃ temperature condition all the time, experiment special gas generation control device exposes the experiment chamber by air feed branch pipe and cell gaseous state and links to each other to fetch and finish various types of cells gaseous state exposure exposure experiment.
2. cell gaseous state according to claim 1 exposes experiement control device, it is characterized in that: described cell gaseous state exposes the experiment chamber and links to each other with the cell cultures sap cavity, places the micropore lamina membranacea of experiment with the cell special use between these two continuous cavitys.
3. cell gaseous state according to claim 1 exposes experiement control device, it is characterized in that: the cell cultures liquid supply unit keeps cell culture fluid to soak into micropore lamina membranacea bottom surface constantly offering the required nutrition of experimental cell growth, and the cell upper surface on the micropore template is directly exposed to gaseous state again and exposes and accept gaseous state in the chamber and expose contamination simultaneously.
4. cell gaseous state according to claim 1 exposes experiement control device, it is characterized in that: the temperature that the described temperature that is close on cell gaseous state exposure experiment chamber and these two cavitys of cell cultures sap cavity is bathed control device control is bathed chamber assurance experimental cell normal growth under 37 ℃ temperature condition all the time.
5. cell gaseous state according to claim 1 exposes experiement control device, it is characterized in that: described experiment special gas generation control device is sent into cell gaseous state exposure experiment chamber with the experimental gas of different concns via the air feed branch pipe by gas exposure concentrations controller and gas flow controller and is finished various types of cells gaseous state exposure exposure experiment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102702439A CN102010821A (en) | 2010-09-02 | 2010-09-02 | Cell gaseous exposure experiment control device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102702439A CN102010821A (en) | 2010-09-02 | 2010-09-02 | Cell gaseous exposure experiment control device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102010821A true CN102010821A (en) | 2011-04-13 |
Family
ID=43841154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102702439A Pending CN102010821A (en) | 2010-09-02 | 2010-09-02 | Cell gaseous exposure experiment control device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102010821A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199532A (en) * | 2011-04-14 | 2011-09-28 | 天津开发区合普工贸有限公司 | Cell phototoxicity experimental detection device |
| CN102337210A (en) * | 2011-10-10 | 2012-02-01 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN102443534A (en) * | 2011-10-14 | 2012-05-09 | 天津开发区合普工贸有限公司 | Cell intermittent hypoxic simulation experiment equipment |
| CN103525699A (en) * | 2013-10-22 | 2014-01-22 | 解放军第210医院 | Special gas accurate controller for cell exposure |
| CN106442250A (en) * | 2016-08-01 | 2017-02-22 | 上海伽誉生物科技有限公司 | Anti-pollution testing device and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5945338A (en) * | 1995-06-26 | 1999-08-31 | Vivorx Pharmaceuticals | Method and apparatus for augmenting mass transfer in a rotating cell culture system |
| US20060258000A1 (en) * | 2003-02-26 | 2006-11-16 | Allen Jared W | Use of steady-state oxygen gradients to modulate animal cell functions |
| CN1894396A (en) * | 2003-12-16 | 2007-01-10 | 赫帕霍普公司 | Drug testing system with bio-artificial liver |
| CN101570739A (en) * | 2009-06-05 | 2009-11-04 | 刘晓明 | Method for building psoriasis basic research models, and gas-liquid level transmembrane device |
-
2010
- 2010-09-02 CN CN2010102702439A patent/CN102010821A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5945338A (en) * | 1995-06-26 | 1999-08-31 | Vivorx Pharmaceuticals | Method and apparatus for augmenting mass transfer in a rotating cell culture system |
| US20060258000A1 (en) * | 2003-02-26 | 2006-11-16 | Allen Jared W | Use of steady-state oxygen gradients to modulate animal cell functions |
| CN1894396A (en) * | 2003-12-16 | 2007-01-10 | 赫帕霍普公司 | Drug testing system with bio-artificial liver |
| CN101570739A (en) * | 2009-06-05 | 2009-11-04 | 刘晓明 | Method for building psoriasis basic research models, and gas-liquid level transmembrane device |
Non-Patent Citations (2)
| Title |
|---|
| M. AUFDERHEIDE ET.AL: "A method for in vitro analysis of the biological activityof complex mixtures such as sidestream cigarette smoke", 《EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY》, vol. 53, 31 December 2001 (2001-12-31), XP004956231, DOI: 10.1078/0940-2993-00187 * |
| 刘海云: "香烟烟雾与氡对 BEAS-2B 细胞的损伤效应", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑（月刊）》, no. 9, 15 September 2009 (2009-09-15) * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199532A (en) * | 2011-04-14 | 2011-09-28 | 天津开发区合普工贸有限公司 | Cell phototoxicity experimental detection device |
| CN102337210A (en) * | 2011-10-10 | 2012-02-01 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN102337210B (en) * | 2011-10-10 | 2013-01-23 | 天津开发区合普工贸有限公司 | Positive and negative pressure compatible cell exposure aseptic shielding cabinet equipment |
| CN102443534A (en) * | 2011-10-14 | 2012-05-09 | 天津开发区合普工贸有限公司 | Cell intermittent hypoxic simulation experiment equipment |
| CN103525699A (en) * | 2013-10-22 | 2014-01-22 | 解放军第210医院 | Special gas accurate controller for cell exposure |
| CN103525699B (en) * | 2013-10-22 | 2015-03-04 | 中国人民解放军第二一〇医院 | Special gas accurate controller for cell exposure |
| CN106442250A (en) * | 2016-08-01 | 2017-02-22 | 上海伽誉生物科技有限公司 | Anti-pollution testing device and method |
| CN106442250B (en) * | 2016-08-01 | 2020-07-03 | 上海伽誉生物科技有限公司 | Anti-pollution testing device and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Stanley et al. | Dual‐flow‐RootChip reveals local adaptations of roots towards environmental asymmetry at the physiological and genetic levels | |
| US20160053214A1 (en) | Bioreactor, devices, systems and methods | |
| EA201892695A1 (en) | DISPOSABLE BIOREACTOR | |
| CN102010821A (en) | Cell gaseous exposure experiment control device | |
| EP0073773B1 (en) | Perfusion-cultivation of animal cells and equipment therefor | |
| CN106318866A (en) | Small multi-purpose cell culturing and testing device | |
| CN204393035U (en) | The plant that a kind of water planting, sand culture combine is without native culture apparatus | |
| ATE376583T1 (en) | METHOD FOR CULTIVATION OF CELLS, PARTICULARLY HUMAN OR ANIMAL CELLS | |
| CN104719119B (en) | A kind of application method for being used to expand the equipment of phytoplankton | |
| CN102172178B (en) | Method for testing influence of different carbon dioxide concentrations on growth of submerged plants | |
| CN201850277U (en) | Controller for cell gaseous exposure experiment | |
| CN102061258B (en) | Cell exposed anaerobic environment experiment device | |
| CN102443534B (en) | Cell intermittent hypoxic simulation experiment equipment | |
| CN102010823A (en) | Cell culture solution flow supply device | |
| KR20160083260A (en) | Farming facility for aquatic organisms based on improved aquaculture environment by controlling biochemical factors | |
| CN104726339B (en) | A kind of immobilization cultural method of microalgae | |
| CN201842846U (en) | Control device of cell exposure gas with controllable concentration | |
| CN216585031U (en) | Zebra fish gradient dissolved oxygen experimental apparatus | |
| CN102010818A (en) | Control device of cell exposing gas with controllable concentration | |
| CN104743711B (en) | Ballast water for ship land-based test flows into the biological parameter modulator approach of water | |
| CN102010822A (en) | Cell culture fluid atomizing supply device | |
| KR20150006718A (en) | Microalgal Cultivation Facility Using Closed Carbon Dioxide Photobioreactor | |
| CN201933085U (en) | Cell-exposure oxygen-deficient environment experimental device | |
| CN201825958U (en) | Cell culture solution flowing supply device | |
| RU2626526C1 (en) | System of animal and human tissue bio-engineering models creation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110413 |