CN208917211U - A kind of lift single cell clone culture box - Google Patents
A kind of lift single cell clone culture box Download PDFInfo
- Publication number
- CN208917211U CN208917211U CN201821258678.XU CN201821258678U CN208917211U CN 208917211 U CN208917211 U CN 208917211U CN 201821258678 U CN201821258678 U CN 201821258678U CN 208917211 U CN208917211 U CN 208917211U
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- Prior art keywords
- placement platform
- single cell
- cell clone
- rib
- culture
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Abstract
The utility model discloses a kind of lift single cell clone culture boxes, have elevating mechanism, and elevating mechanism includes circular rails, puts body and rotating part；Circular rails side is provided with guide rail；Body is put to include symmetrically arranged two semicircle placement platform one and placement platforms two and be arranged between placement platform one and placement platform two and stir slot；It is provided with several unicellular culture columns in placement platform one and placement platform two, the side of placement platform one and placement platform two is symmetrically arranged with protruded stigma, and protruded stigma is mobilizable to be plugged in guide rail；Rotating part includes flange and stirs rib, it is vertical with flange and be fixed at the diameter line of flange to stir rib, it stirs rib and is plugged in and stir in slot, rotary flange can make to stir the dynamic body of putting of ribbed belt and slowly rise along guide rail, so that putting the storing body of unicellular culture column can be freely lifted, structure is simple and easy to use, has very high application value.
Description
Technical field
The utility model relates to bioengineering field, more particularly to a kind of lift single cell clone culture
Box.
Background technique
In today of life science rapid development, the selection of specific monoclonal cell strain (being) and cultivate increasingly extensive and again
It wants, the in vitro culture of cell and tissue has become the important investigative technique in life studies field.Without special installation, operation letter
The technology of single, quick, economic, efficient, reliable and suitable high-volume colonized culture is beneficial to the simplification of this hard work
With it is universal.
Be formed by cell mass after procreation by single a cell and claim clone cell group, no matter therefore how gram
Grand cell, it is necessary to guarantee it is separated come out as cloning cell be really one rather than two or more, that is to say, that
This group cell origin is in a common progenitor cells.So far, for single cell clone culture, there are no it is special more
Perfect cell culture apparatus.
There are unlimited dilution method, plate clone and separate method, self-control capillary syring for the technology of single cell clone culture at present
The cell clones technology such as unicellular micrurgy, wherein most common single cell clone cultural method is that cell is carried out nothing
Individual cells, i.e., be put in 96 orifice plates and cultivate by limit dilution, to realize that the independent growths of individual cells become monoclonal.For not
Colony Culture with growth pattern cell (growth and adherent growth etc. under growth, half suspended state under suspended state) exists
Some difficulties:
A) this method of is cumbersome, and the clone group obtained is also few；
B) in screening transgenic cell, the transfection efficiency of many cells is low, only obtains the seldom positive cell of quantity
When, with micropipettor absorption select monoclonal cell when, on the one hand, may it is improper operation and cause draw cell not only
One unicellular and pollute, and the single source of clone cannot be completely secured, on the other hand, it is also possible in suction process
In, culture medium has been shaken, has caused to lose the positive cell in the visual field；
C) for during Colony Culture, the nutrition and other factors that the growth and breeding process of cell needs are not only complete
Portion comes from culture medium, around the material molecule also outwardly secreted in own cells population development promotes and itself
Growth and breeding, and infinite dilution method in very big environment only have an independent cell, cell itself secretion it is some because
Son is the flourish for being highly detrimental to it by extreme dilution；
D) it to carry out changing liquid process, such as suspension cell in incubation, when carrying out changing liquid, may be sucked away, because
It is extremely difficult to carry out changing liquid operation in this existing culture dish or 96 orifice plates, and is easily destroyed cell.
Therefore how to provide and a kind of be conducive to cell growth and breeding and just with screening and easy to operate single cell clone culture box
It is those skilled in the art's urgent problem to be solved.
Utility model content
In view of this, the present invention provides a kind of Liftable type single cell clone culture boxes, using rotatable rising
Holder carry unicellular culture column, and unicellular culture column has semi-transparent membrane material, can easily by cell with cultivate
Liquid separation.
To achieve the goals above, the utility model adopts the following technical solution:
A kind of lift single cell clone culture box, comprising: pedestal, elevating mechanism and upper cover；
The pedestal is the round box that top full-open type has shallow storage space, and the top of the pedestal connects the lifting
Mechanism；
The elevating mechanism includes circular rails, puts body and rotating part；
The circular rails bottom end connects the pedestal, and top connects the rotating part, the circular rails side be provided with
Plane has the guide rail of angle；
The storing body includes symmetrically arranged two semicircle placement platforms one and placement platform two and is arranged in placement platform
Slot is stirred between one and placement platform two；
Several unicellular culture columns, the placement platform one and placement platform are provided in the placement platform one and placement platform two
Two side is symmetrically arranged with protruded stigma, and the protruded stigma is mobilizable to be plugged in the guide rail；
The rotating part includes flange and stirs rib, and the flange is the top of ring structure and bottom end and the circular rails
It is flexibly connected, the rib of stirring is vertical with the flange and be fixed at the diameter line of the flange, and the rib of stirring is plugged in
It is described to stir in slot；
The internal diameter of the upper cover is greater than the outer diameter of the pedestal.
Further, the cylinder of the unicellular culture column includes vertical sequentially connected support portion one, nutrition exchange part
With support portion two.
Further, the nutrition exchange part includes that semi-permeable membrane and both ends are individually fixed on support portion one and support portion two
Ribs.
Preferably, the area of the nutrition exchange part is the one third of the unicellular culture column gross area.
Preferably, the height of the pedestal and the circular rails be all larger than or equal to the unicellular culture column height,
Unicellular culture column can be made to be completely submerged in culture solution, guarantee cell eubolism.
Preferably, the height for stirring rib is less than the height of the circular rails, it is ensured that placement platform one and two liang of placement platform
The uniformity of the culture solution at place.
Preferably, the height for stirring rib is equal to the height of the circular rails.
Preferably, described stir is provided with equal discharge orifice on rib, guarantees the culture solution at placement platform one and two liang of placement platform
Connection, guarantees the uniformity of culture solution.
Preferably, the side of the placement platform one is provided with pipettor insert port one, the side setting of the placement platform two
There is pipettor insert port two.
Preferably, the guide rail and plane included angle are 15-30 °.
As can be seen from the above technical solutions, compared with prior art, a kind of lift disclosed by the utility model is unicellular
Clone's culture box has the advantage that
1) the storing body for putting unicellular culture column can be freely lifted, and one third in the middle part of the peripheral wall of unicellular culture column
Material be made of semi-permeable membrane, cell culture medium, cell factor, nutritional ingredient, carbon dioxide, oxygen etc. can be freely through
Semi-transparent membrane structure enters convenient for culture solution for cell function and can quick separating cell and culture solution；
2) for holding the culture single chambers of individual cells, it is very small that volume compares 96 orifice plates, positions convenient for individual cells, and
Facilitate under the microscope；
3) this culture box can be not only adapted to this field auto sample applicator, can also quickly be grasped using common pipettor
Make；
4) more suitable cell suspension can be added in the biggish accommodating culture medium space of pedestal, more comprehensive to single
Indoor monoclonal cell provides nutriment.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is the embodiments of the present invention, for those of ordinary skill in the art, without creative efforts, also
Other attached drawings can be obtained according to the attached drawing of offer.
Fig. 1 attached drawing is a kind of external overall schematic of lift single cell clone culture box of the utility model；
Fig. 2 attached drawing is a kind of inside overall structure diagram of lift single cell clone culture box of the utility model；
Fig. 3 attached drawing is a kind of unicellular culture rod structure figure of lift single cell clone culture box of the utility model；
Fig. 4 attached drawing is a kind of modular construction dendrogram of lift single cell clone culture box of the utility model；
In figure: 1, pedestal, 11, shallow storage space, 2, elevating mechanism, 21, circular rails, 211, guide rail, 22, storing body, 221,
Placement platform one, 2211, pipettor insert port one, 222, placement platform two, 2221, pipettor insert port two, 223, stir slot, 224,
Unicellular culture column, 2241, support portion one, 2242, nutrition exchange part, 2243, support portion two, 2244, semi-permeable membrane, 2245, branch
Stake rib, 225, protruded stigma, 23, rotating part, 231, flange, 232, stir rib, 2321, equal discharge orifice, 3, upper cover.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
The utility model provides a kind of miniature laser direction fixed frame, utilizes two axis running fix tune of laser frame reversing gear bracket
Solidating is fixed and two axis are directed toward orientation rotation and adjust, and it is firm to be fixed conveniently, and locks after can carrying out micro adjustment, adjustment, horizontal can pacify
Dress it is fixed can also vertically-mounted fixation, meet comprehensive direction.
Embodiment 1
Referring to 1~attached drawing of attached drawing 3, a kind of lift single cell clone culture box, comprising: pedestal 1, elevating mechanism 2 and on
Lid 3；
Pedestal 1 is the round box that top full-open type has shallow storage space 11, and the top of pedestal 1 connects elevating mechanism 2；
Elevating mechanism 2 includes circular rails 21, puts body 22 and rotating part 23；
21 bottom end of circular rails connects pedestal 1, and top connects rotating part 23, and 21 side of circular rails, which is provided with, has folder with plane
The guide rail 211 at angle；
Putting body 22 includes that symmetrically arranged two semicircle placement platforms 1 and placement platform 2 222 and setting are being set
It puts and stirs slot 223 between platform 1 and placement platform 2 222；
It is provided with several unicellular culture columns 224 in placement platform 1 and placement platform 2 222, placement platform 1 and sets
The side for putting platform 2 222 is symmetrically arranged with protruded stigma 225, and protruded stigma 225 is mobilizable to be plugged in guide rail 211；
Rotating part 23 includes flange 231 and stirs rib 232, and flange 231 is the top of ring structure and bottom end and circular rails 21
End is flexibly connected, and it is vertical with flange 231 and be fixed at the diameter line of flange 231 to stir rib 232, is stirred rib 232 and is plugged in group
In dynamic slot 223；
The internal diameter of upper cover 3 is greater than the outer diameter of pedestal 1.
In order to advanced optimize above scheme, the cylinder of unicellular culture column 224 includes vertical sequentially connected support portion
One 2241, nutrition exchange part 2242 and support portion 2 2243.
In order to advanced optimize above scheme, nutrition exchange part 2242 includes that semi-permeable membrane 2244 and both ends are individually fixed in branch
Ribs 2245 on support part 1 and support portion 2 2243.
In order to advanced optimize above scheme, the area of nutrition exchange part 2242 is unicellular culture 224 gross area of column
One third.
In order to advanced optimize above scheme, the height of pedestal 1 and circular rails 21 is all larger than or is equal to unicellular culture column
224 height.
In order to advanced optimize above scheme, the height for stirring rib 232 is less than the height of circular rails 21.
In order to advanced optimize above scheme, the height for stirring rib 232 is equal to the height of circular rails 21.
In order to advanced optimize above scheme, stirs and be provided with equal discharge orifice 2321 on rib 232.
In order to advanced optimize above scheme, the side of placement platform 1 is provided with pipettor insert port 1, puts
The side of platform 2 222 is provided with pipettor insert port 2 2221.
In order to advanced optimize above scheme, the guide rail 211 is 15~30 ° with plane included angle.
In culture use process, upper cover is removed first, it can be seen that placement platform 1 and 2 222 surface of placement platform are gone into battle
The column mouth of the unicellular culture column 224 of column arrangement, passes through the pipettor insert port 1 and placement platform two of one 221 side of placement platform
Appropriate co-cultured cell suspension or fresh culture is added with pipettor in the pipettor insert port 2 2221 of 222 sides；Then,
It stirs rib 232 and increases storing body 22, it is convenient clearly to observe unicellular 224 liquid feeding situation of culture column, contain what is taken
Single monoclonal cell suspension injects in single unicellular culture column 224, then stirs rib 232 and declines the storing body 22,
It can observe that unicellular culture column 224 is immersed in the liquid in the accommodating space of pedestal 1 by outer box wall, cover upper cover 3, put
In in cell incubator, normally cultivate.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments can be realized professional and technical personnel in the field or using originally practical new
Type.Various modifications to these embodiments will be readily apparent to those skilled in the art, and determine herein
The General Principle of justice can be realized in other embodiments without departing from the spirit or scope of the present utility model.Cause
This, the present invention will not be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The widest scope consistent with features of novelty.
Claims (10)
1. a kind of lift single cell clone culture box characterized by comprising pedestal (1), elevating mechanism (2) and upper cover
(3)；
The pedestal (1) is the round box that top full-open type has shallow storage space (11), and the top of the pedestal (1) connects institute
State elevating mechanism (2)；
The elevating mechanism (2) includes circular rails (21), puts body (22) and rotating part (23)；
Circular rails (21) bottom end connects the pedestal (1), and top connects the rotating part (23), circular rails (21) side
Face is provided with the guide rail (211) for having angle with plane；
The storing body (22) includes symmetrically arranged two semicircle placement platforms one (221) and placement platform two (222) and sets
It sets and stirs slot (223) between placement platform one (221) and placement platform two (222)；
Several unicellular culture columns (224), the storing are provided on the placement platform one (221) and placement platform two (222)
The side of platform one (221) and placement platform two (222) is symmetrically arranged with protruded stigma (225), and the protruded stigma (225) is mobilizable to be plugged on
In the guide rail (211)；
The rotating part (23) includes flange (231) and stirring rib (232), the flange (231) be ring structure and bottom end with
The tops of the circular rails (21) is flexibly connected, and the rib (232) of stirring is vertical with the flange (231) and be fixed on described convex
At the diameter line of edge (231), the rib (232) of stirring is plugged in described stir in slot (223)；
The internal diameter of the upper cover (3) is greater than the outer diameter of the pedestal (1).
2. a kind of lift single cell clone culture box according to claim 1, which is characterized in that the unicellular culture
The cylinder of column (224) includes vertical sequentially connected support portion one (2241), nutrition exchange part (2242) and support portion two
(2243)。
3. a kind of lift single cell clone culture box according to claim 2, which is characterized in that the nutrition exchange part
It (2242) include the support that semi-permeable membrane (2244) are individually fixed on support portion one (2241) and support portion two (2243) with both ends
Rib (2245).
4. a kind of lift single cell clone culture box according to claim 3, which is characterized in that the nutrition exchange part
(2242) area is the one third of described unicellular culture column (224) gross area.
5. a kind of lift single cell clone culture box according to claim 1, which is characterized in that the pedestal (1) with
The height of the circular rails (21) is all larger than or is equal to the height of unicellular culture column (224).
6. a kind of lift single cell clone culture box according to claim 5, which is characterized in that described to stir rib
(232) height is less than the height of the circular rails (21).
7. a kind of lift single cell clone culture box according to claim 5, which is characterized in that described to stir rib
(232) height is equal to the height of the circular rails (21).
8. a kind of lift single cell clone culture box according to claim 7, which is characterized in that described to stir rib
(232) equal discharge orifice (2321) are provided on.
9. a kind of lift single cell clone culture box according to claim 7, which is characterized in that the placement platform one
(221) side is provided with pipettor insert port one (2211), and the side of the placement platform two (222) is provided with pipettor insertion
Two (2221) of mouth.
10. described in any item a kind of lift single cell clone culture boxes according to claim 1~9, which is characterized in that described
Guide rail (211) and plane included angle are 15-30 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821258678.XU CN208917211U (en) | 2018-08-06 | 2018-08-06 | A kind of lift single cell clone culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821258678.XU CN208917211U (en) | 2018-08-06 | 2018-08-06 | A kind of lift single cell clone culture box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208917211U true CN208917211U (en) | 2019-05-31 |
Family
ID=66702372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821258678.XU Expired - Fee Related CN208917211U (en) | 2018-08-06 | 2018-08-06 | A kind of lift single cell clone culture box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208917211U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438006A (en) * | 2019-09-20 | 2019-11-12 | 桂林医学院 | A kind of adherent unicellular point of Double layer culture device |
| CN111334430A (en) * | 2020-03-27 | 2020-06-26 | 济南磐升生物技术有限公司 | Double-layer artificial skin culture device and use method thereof |
-
2018
- 2018-08-06 CN CN201821258678.XU patent/CN208917211U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438006A (en) * | 2019-09-20 | 2019-11-12 | 桂林医学院 | A kind of adherent unicellular point of Double layer culture device |
| CN110438006B (en) * | 2019-09-20 | 2024-03-01 | 桂林医学院 | Wall-attached single-cell double-layer culture device |
| CN111334430A (en) * | 2020-03-27 | 2020-06-26 | 济南磐升生物技术有限公司 | Double-layer artificial skin culture device and use method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190531 Termination date: 20200806 |
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| CF01 | Termination of patent right due to non-payment of annual fee |