CN110438006A - A kind of adherent unicellular point of Double layer culture device - Google Patents
A kind of adherent unicellular point of Double layer culture device Download PDFInfo
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- CN110438006A CN110438006A CN201910895033.XA CN201910895033A CN110438006A CN 110438006 A CN110438006 A CN 110438006A CN 201910895033 A CN201910895033 A CN 201910895033A CN 110438006 A CN110438006 A CN 110438006A
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- 230000001464 adherent effect Effects 0.000 title claims abstract description 43
- 239000011521 glass Substances 0.000 claims abstract description 34
- 239000007787 solid Substances 0.000 claims abstract description 18
- 235000005121 Sorbus torminalis Nutrition 0.000 claims description 4
- 244000152100 Sorbus torminalis Species 0.000 claims description 4
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000011160 research Methods 0.000 abstract description 7
- 238000013461 design Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 45
- 238000000034 method Methods 0.000 description 5
- 108010019160 Pancreatin Proteins 0.000 description 4
- 230000011278 mitosis Effects 0.000 description 4
- 229940055695 pancreatin Drugs 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 239000002547 new drug Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010028347 Muscle twitching Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of adherent unicellular point of Double layer culture devices, including slide glass, inserted sheet, plectrum, drive item, carrier and solid column, the drive item is located at device the top, lower section is plectrum, the inserted sheet includes inserted sheet flank, top cover and hinge area, bottom have prevent double-vane adhesion support column, inserted sheet flank is connected through hinge area with top cover, the slide glass is hollow slide, including carrying slot, edge hole, carrying slot bracket, outline border and up and down support rib in slide glass, the solid column is the long square column of fixed slide glass, and the carrier is the stereoscopic frame for including edge posts and solid column card slot.The complex appts of the adherent unicellular point of Double layer culture of the present invention, with terrestrial gravitation and attached cell adherent growth characteristic, by it is adherent it is unicellular divide resulting double cells for the first time and be uprightly incubated for and stand upside down to cultivate be attached on the inserted sheet flank in mirror image.The apparatus structure is fine, ingenious in design, easy to operate, divides Double layer culture high-efficient, is convenient for laboratory research and sterile working.
Description
Technical field
The invention belongs to general chemistry or physics laboratory's equipment technical field, in particular to a kind of adherent unicellular point double
Layer culture apparatus.
Background technique
Attached cell (Adherent cells) is a kind of by itself secretion, culture solution offer or support surface coating
Adhesion factor could support surface grow, breeding eukaryotic cell lines.The growth course of attached cell is broadly divided into trip
From phase, adherent phase, incubation period, logarithmic phase, plateau and decline phase, epithelial cell sample or fibroblast are generally formed after adherent
Sample growthform.Attached cell has become research vaccine, developing new drug, preparation monoclonal antibody, parsing gene function and medicine
The object mechanism of action, in vitro culture pathogenic microorganism and the weight for verifying disease development mechanism and attached cell own activity rule
Lab material is wanted, scientific research value is not sneezed at.
Currently, common attached cell cultural method are as follows: blown and beaten into after pancreatin digests after passage cell unicellular outstanding
Liquid is then spread by specified inoculative proportion into cell bottle or culture plate, is placed in cell incubator and is continued to seek adherent growth, until reaching
To experiment demand or it is covered with support bottom surface.It is not difficult to find that being in fact exactly to count with the above-mentioned resulting attached cell of passage mode
The unknown adherent unicellular cell mass obtained through polymitosis of mesh is spread into several and mitosis number not except ancestral cell is played
With outer, in each cell bottle or culture plate it is adherent it is unicellular between iuntercellular away from also unequal.Gained attached cell seems still
One form cell colony more clear than more uniform, the more stable, biological character of growth, in fact its propagating active and new old
Metabolic capability is obviously different.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of adherent unicellular point of Double layer culture device, including slide glass, insert
Piece, plectrum drive item, carrier and solid column, and the drive item is located above device, including item head and body；The plectrum, which is located at, drives item
Lower section, including sheet body, clamping part, plectrum ring, column spinner and rotary column pedestal, plectrum ring are the buckle for connecting inserted sheet, and plectrum can phase
Inserted sheet is rotated, sheet body is the slide for stirring inserted sheet flank；The inserted sheet includes inserted sheet flank, top cover and hinge area, flank bottom
There is the column that supports for preventing double-vane adhesion in portion, and pressure rib is located inside above flank, highly identical as top pillar, inserted sheet flank through hinge area and
Top cover is connected；The slide glass is hollow slide, including carries slot, edge hole, carry slot bracket, outline border and support rib, side up and down in slide glass
Marginal pore is to support slide glass, inserted sheet is auxiliarily fixed and keeps the square column of adjacent slide glass spacing for being inserted into edge posts, support rib；The solid column
For the long square column of fixed slide glass；The carrier is the stereoscopic frame for including edge posts and solid column card slot.
Preferably, the item that drives is the opposite biplate item of the direction of motion, and item head is grips, and there is anti-skid chequer on surface, body
There is round hole, quantity is identical as driven plectrum quantity.
Preferably, the column spinner of the plectrum is the pillar type column for being inserted into a hole, and highly identical as thickness is driven, diameter is less than
Rotating base；The outer diameter of column spinner is slightly less than the internal diameter in hole, and driving can rotate between item and plectrum around column spinner.
Preferably, the retaining part of the plectrum is highly identical as rotating base among the top of plectrum, the clamping
Portion lifts for hand clamping.
Preferably, there are two plectrum jack in the middle part of the top cover, respectively there is a round top pillar in quadrangle.
Preferably, the inserted sheet flank is the left and right double-vane in mirror image, and double-vane medial surface has attached cell to stick area.
Preferably, the load slot is the U-shaped gool for being only capable of carrying single attached cell.
The present invention provides one kind to divide Double layer culture adherent single celled new equipment, and a kind of adherent unicellular point of bilayer
The new feasible scheme of culture.
The present invention is designed according to terrestrial gravitation and attached cell adherent growth characteristic, first immerses the carrier equipped with slide glass
In attached cell suspension, taken out after acting on 3-5s；It is inserted into inserted sheet and plectrum, loads onto and drives item and solid column, after cell is completely adherent
Handstand culture apparatus to mitosis for the first time is completed；Pancreatin takes out solid column after digesting double Cell trackings, and forward direction extracts inserted sheet, exhibition
Open culture.Structure of the invention is fine, ingenious in design, and adherent unicellular point of bilayer is high-efficient, is convenient for laboratory research and nothing
Bacterium operation.
Detailed description of the invention
A specific embodiment of the invention is described in further detail with reference to the accompanying drawing.
Fig. 1 is the perspective view of the adherent unicellular point of Double layer culture device of the embodiment of the present invention
Fig. 2 is the top view of the adherent unicellular point of Double layer culture device of the embodiment of the present invention.
Fig. 3 is the perspective view of the adherent unicellular point of Double layer culture device of the embodiment of the present invention.
Fig. 4 is the cross-sectional of the adherent unicellular point of Double layer culture device of the embodiment of the present invention.
Fig. 5 is the top view of slide glass.
Fig. 6 is the longitudal section and cross-sectional of slide glass.
Fig. 7 is setting, outreach and the outreach schematic top plan view of inserted sheet.
Fig. 8 is the perspective view of plectrum.
Fig. 9 is the longitudal section schematic diagram of plectrum.
Figure 10 is the structural schematic diagram for driving item.
Figure 11 is the perspective view of solid column.
Figure 12 is the perspective view of carrier.
Figure 13 is the operating process schematic diagram of the adherent unicellular point of Double layer culture device of the embodiment of the present invention.
In figure, 1: slide glass；2: inserted sheet；3: plectrum；4: driving item；5: carrier；6: Gu column；11: carrying slot；12: carrying slot bracket；
13: slide glass inside casing；14: above supportting rib；15: lower to support rib；16: slide glass outline border；17: edge hole；18: cell supporting region；21: inserted sheet side
The wing；22: supporting column；23: pressure rib；24: top cover；25: top pillar；26: plectrum jack；27: sticking area；28: hinge area；31: sheet body；
32: clamping part；33: plectrum ring；34: column spinner；35: rotary column pedestal；41: item head；42: body；43: hole；44: anti-skid chequer；
51: edge posts；52: Gu column card slot.
Specific embodiment
As shown in figures 1-13, the invention discloses a kind of adherent unicellular point of Double layer culture devices, are a kind of double for dividing
Layer cultivates the adherent single celled combined container of slide.
It is described adherent for by cell itself secretion, the adhesion factor ability that culture solution provides or cell substrates surface is coated with
It can be grown in support surface, the characteristic of breeding.
The unicellular resulting single cell population after pancreatin digests of the attached cell for wait pass on.
Described point of Double layer culture is to have silk point for the first time for unicellular with terrestrial gravitation and attached cell adherent growth characteristic
Split the training method that resulting double cells are in mirror image separation.
The container is nature of glass container.
A kind of adherent unicellular point of Double layer culture device, including slide glass 1, inserted sheet 2, plectrum 3, drive item 4, carrier 5 and solid column
6, the drive item 4 is located above device, including item head 41 and body 42；The plectrum 3, which is located at, drives 4 lower section of item, including sheet body 31,
Clamping part 32, plectrum ring 33, column spinner 34 and rotary column pedestal 35, plectrum ring 33 are the buckle for connecting inserted sheet 2, and plectrum can be inserted relatively
Piece rotation, sheet body 31 is the slide for stirring inserted sheet flank 21；The inserted sheet 2 includes inserted sheet flank 21, top cover 24 and hinge area 28,
Flank bottom has prevent double-vane adhesion to support column 22, on the inside of pressure rib 23 is located above flank, highly identical as top pillar 25, inserted sheet side
The wing 21 is connected through hinge area 28 with top cover 24, and hinge can be the soft material of energy inflection；Sheet body lower part be located at top cover 24 in the following, with
In stirring inserted sheet flank 21；The slide glass 1 is inside and outside hollow slide, including load slot 11, edge hole 17, load slot bracket 12, slide glass
Frame 13,16 and up and down support rib 14,15, edge hole 17 are support slide glass 1,2 and of inserted sheet are auxiliarily fixed for being inserted into edge posts 51, support rib
Keep the square column of adjacent 1 spacing of slide glass；The solid column 6 is the long square column of fixed slide glass 1；The carrier 5 be include edge posts 51
With the stereoscopic frame of solid column card slot 52.
The item 4 that drives is the opposite biplate item of the direction of motion, and item head 41 is grips, and there is anti-skid chequer 44 on surface, body 42
There is round hole 43, quantity is identical as driven 3 quantity of plectrum.
The column spinner 34 of the plectrum 3 is the pillar type column for being inserted into a hole 43, and highly identical as 4 thickness of item is driven, diameter is less than
Rotating base；The outer diameter of column spinner 34 is slightly less than the internal diameter in hole 43, and driving can rotate between item and plectrum around column spinner.
The clamping part 32 of the plectrum 3 is located among the top of plectrum 3, highly identical as rotating base, the clamping part
32 lift for hand clamping.
There are two plectrum jack 26, respectively there is a round top pillar 25 in quadrangle at 24 middle part of top cover.
The inserted sheet flank 21 is the left and right double-vane in mirror image, and double-vane medial surface has attached cell to stick area 27.
The load slot 11 is the U-shaped gool for being only capable of carrying single attached cell.
Operating process is as follows:
1, the preparation of adherent single cell suspension；
1) when attached cell is covered with support bottom surface, top culture solution is removed, suitable 0.25% trypsin solution is added,
Place CO2In incubator, digestion to cell is completely fallen off.
2) appropriate cell culture fluid (containing 10% fetal calf serum and 1 × dual anti-) is added, mixes gently, piping and druming to unicellular shape
State moves into spare in sterile 50mL centrifuge tube, 6 holes or 12 well culture plates.
2, the assembling, disassembly and cell culture of invention device；
1) slide glass 1 is sequentially loaded into carrier 5 along edge posts 51, the carrier equipped with slide glass is uprightly immersed above-mentioned unicellular outstanding
In liquid, taken out after acting on 3-5s.
2) inserted sheet double-vane is carefully inserted into lower support rib side channel (lower region between support rib and slide glass inside casing 13), loading onto plectrum 3 (makes
31 direction of sheet body is parallel with 2 flank of inserted sheet) after be pushed down to 5 bottom of carrier.
3) it is then indicated according to Figure 13, inserted sheet 2 is pushed into forward upper support rib 14,2 double-vane of inserted sheet is held with sterile tweezer, makes it
Slot 11 is carried tight against centre.
4) inserted sheet 2 is pushed forward into upper 14 side channel of support rib (above supportting region between rib 14 and load slot bracket 12), solid column 6 is inserted into
Gu after column card slot 52, continuing to be incubated for culture in Yu Shiliang cell culture fluid (containing 10% fetal calf serum and 1 × dual anti-).
5) after cell is completely adherent, device is inverted and is cultivated to cell mitosis for the first time and is completed.
6) with after the double Cell trackings of appropriate pancreatin digestion gained, solid column 6 is taken out, inserted sheet 2 is pulled to lower support rib backward, is filled
It twitches above and to two sides and drives item 4.
7) it when 2 flank of inserted sheet is supported to slide glass inside casing 13, removes and drives item 4, inserted sheet 2 is pulled back into lower support rib side channel.
8) plectrum 3 is taken out, inserted sheet 2 is extracted upwards, inserted sheet 2 is placed in cell bottle or culture plate, with cell adhesion area 27
Upward mode is unfolded to cultivate.
9) according to Research Requirements, when such as needing largely to be in the attached cell of mirror image, can suitably increase inserted sheet flank size and/
Or increase slide glass 1,2 quantity of inserted sheet.
Attached cell is a kind of provides by itself secretion, culture solution or the adhesion factor of support surface coating could be
The eukaryotic cell lines of support surface growth, breeding have become research vaccine, developing new drug, preparation monoclonal antibody, parsing
Gene function and drug mechanism, in vitro culture pathogenic microorganism and verify disease development mechanism and attached cell itself
The important lab material of mechanics.With traditional passage mode, resulting attached cell is unknown adherent of number in fact
The unicellular cell mass obtained through polymitosis, except rise an ancestral cell spread number it is different with mitosis number in addition to, each cell
In bottle or culture plate it is adherent it is unicellular between iuntercellular away from also unequal, cause the propagating active of gained attached cell and new old
Metabolic capability is obviously different.The present invention provides a kind of complex appts of adherent unicellular point of Double layer culture, with terrestrial gravitation
With attached cell adherent growth characteristic, by it is adherent it is unicellular divide resulting double cells for the first time and be uprightly incubated for and stood upside down culture stick
It is attached on the inserted sheet flank in mirror image.The apparatus structure is fine, ingenious in design, easy to operate, and point bilayer is high-efficient, convenient for experiment
Room research and sterile working.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (7)
1. a kind of adherent unicellular point of Double layer culture device, it is characterised in that: including slide glass (1), inserted sheet (2), plectrum (3), drive
Item (4), carrier (5) and solid column (6), the drives item (4) is located at device top, including item head (41) and body (42)；Described group
Piece (3), which is located at, to be driven below item (4), including sheet body (31), clamping part (32), plectrum ring (33), column spinner (34) and rotary column pedestal
(35), plectrum ring (33) is the buckle for connecting inserted sheet (2), and plectrum can be rotated relative to inserted sheet, and sheet body (31) is to stir inserted sheet flank
(21) slide；The inserted sheet (2) includes inserted sheet flank (21), top cover (24) and hinge area (28), and flank bottom prevents double
Column (22) are supported in wing adhesion, and pressure rib (23) is located inside above flank, and inserted sheet flank (21) is through hinge area (28) and top cover (24)
It is connected；The slide glass (1) is outline border in hollow slide, including load slot (11), edge hole (17), load slot bracket (12), slide glass
(13,16) and up and down support rib (14,15), edge hole (17) is for being inserted into edge posts (51), and support rib is support slide glass (1), auxiliary is solid
Determine inserted sheet (2) and keeps the square column of adjacent slide glass (1) spacing；Described solid column (6) are the long square column of fixed slide glass (1)；The load
Frame (5) is the stereoscopic frame for including edge posts (51) and solid column card slot (52).
2. adherent unicellular point of Double layer culture device according to claim 1, it is characterised in that: the drive item (4) is fortune
Contrary biplate item is moved, item head (41) is grips, and surface has anti-skid chequer (44), and body (42) has round hole (43),
Quantity is identical as driven plectrum (3) quantity.
3. adherent unicellular point of Double layer culture device according to claim 1, it is characterised in that: the rotation of the plectrum (3)
Rotary column (34) is the pillar type column for being inserted into a hole (43), and highly identical as item (4) thickness is driven, diameter is less than rotating base；Column spinner
(34) outer diameter is slightly less than the internal diameter of a hole (43), and driving can rotate between item (4) and plectrum (3) around column spinner.
4. adherent unicellular point of Double layer culture device according to claim 1, it is characterised in that: the folder of the plectrum (3)
It holds portion (32) to be located among the top of plectrum (3), highly identical as rotating base, the clamping part (32) mentions for hand clamping
It rises.
5. adherent unicellular point of Double layer culture device according to claim 1, it is characterised in that: in the middle part of the top cover (24)
There are two plectrum jack (26), respectively there is a round top pillar (25) in quadrangle.
6. adherent unicellular point of Double layer culture device according to claim 1, it is characterised in that: the inserted sheet flank (21)
For in the left and right double-vane of mirror image, double-vane medial surface has attached cell to stick area (27).
7. adherent unicellular point of Double layer culture device according to claim 1, it is characterised in that: the load slot (11) is only
The U-shaped gool of single attached cell can be carried.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910895033.XA CN110438006B (en) | 2019-09-20 | 2019-09-20 | Wall-attached single-cell double-layer culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910895033.XA CN110438006B (en) | 2019-09-20 | 2019-09-20 | Wall-attached single-cell double-layer culture device |
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| CN110438006A true CN110438006A (en) | 2019-11-12 |
| CN110438006B CN110438006B (en) | 2024-03-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980689A (en) * | 2021-02-08 | 2021-06-18 | 湖南美柏生物医药有限公司 | Adherent cell culture device, 2.5D beehive type culture system and method |
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| CN208917211U (en) * | 2018-08-06 | 2019-05-31 | 昆明医科大学 | A kind of lift single cell clone culture box |
| CN210596107U (en) * | 2019-09-20 | 2020-05-22 | 桂林医学院 | Adherent unicellular double-deck culture apparatus that divides |
| CN114426926A (en) * | 2022-01-16 | 2022-05-03 | 重庆理工大学 | Assembled plate tower type adherent cell reactor and culture method of hepatic cells thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112980689A (en) * | 2021-02-08 | 2021-06-18 | 湖南美柏生物医药有限公司 | Adherent cell culture device, 2.5D beehive type culture system and method |
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