CN208809143U - The quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture - Google Patents
The quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture Download PDFInfo
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- CN208809143U CN208809143U CN201820757119.7U CN201820757119U CN208809143U CN 208809143 U CN208809143 U CN 208809143U CN 201820757119 U CN201820757119 U CN 201820757119U CN 208809143 U CN208809143 U CN 208809143U
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- CN
- China
- Prior art keywords
- container
- oscillator
- water washing
- aseptic water
- pulverizer
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Abstract
The utility model discloses the quick chlorination equipments of Isolated Microspore in Chinese Cabbage culture, including bracket, oscillator, container, pipeline, pulverizer, filter device, centrifuge.Bracket is stepped scaffold, five ladders are set, it is alcohol disinfecting platform, mercuric chloride disinfection bench, first time aseptic water washing platform, second of aseptic water washing platform, third time aseptic water washing platform respectively, the height of each ladder is greater than the height that oscillator adds container, and the width of each ladder is 1.5 times of oscillator width.Oscillator is mini-vibrator, has oscillation frequency adjustable and time function.Container is the container of upper opening, lower part side tool liquid outlet, and container diameter is less than or equal to the diameter of oscillator.Pipeline is 90 degree of bendings, and one end connects container outlet, is equipped with switch among pipeline.Pulverizer is placed on the side-lower of third time aseptic water washing platform.Filter device is placed on pulverizer side, and centrifuge is placed on filter device side.Entire disinfection process is all linked with one another, improves work efficiency, greatly reduces pollution probability.
Description
Technical field
The utility model belongs to agricultural biological technical field, and in particular to one kind is fast for Isolated Microspore in Chinese Cabbage culture
Prompt chlorination equipment.
Background technique
Surface sterilization is carried out to test material (bud) before Isolated Microspore in Chinese Cabbage culture, currently, general all super
It is operated on net workbench.Sequence of sterilization are as follows: bud is poured into the alcohol disinfecting after container with 75%, alcohol is outwelled, with 0.1% liter
Mercury disinfection outwells mercuric chloride, then with aseptic water washing, outwells sterile water, and recycle three times.It will ceaselessly be shaken during operation,
To sterilize, rinse thoroughly in place.Bud is crushed after disinfection again, makes the pollen that sheds, using filter and centrifugation, separates impurity, most
After could add culture medium packing.Every subjob continues 3 hours or more and cannot be interrupted, and each process is manual behaviour
Make, there are the problem of: first is that easily polluting, second is that labor intensity is very big, third is that working efficiency is lower.Urgently to existing operation
Process makes improvements.
Summary of the invention
In order to solve the problems existing in the prior art, it is quick to provide a kind of Isolated Microspore in Chinese Cabbage culture for the utility model
Chlorination equipment is carried out disinfection operation with the device, and whole flow process is all linked with one another, and work efficiency is high, and pollution probability is low.
The quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture of the utility model, including bracket, oscillator, container, pipe
Road, pulverizer, filter device, centrifuge.The bracket is stepped scaffold, and five ladders are arranged altogether, are that alcohol disappears respectively
Malicious platform, mercuric chloride disinfection bench, first time aseptic water washing platform, second of aseptic water washing platform, third time aseptic water washing platform, it is each
The height of ladder is greater than the height that oscillator adds container, and the width of each ladder is 1.5 times of oscillator width.The oscillation
Device is mini-vibrator, has the function of oscillation frequency adjustable and time.The container is upper opening, lower part side tool liquid outlet
Container, container diameter be less than or equal to oscillator diameter.The pipeline is 90 degree of bendings, and one end connects container outlet,
Switch is equipped among pipeline.The pulverizer is placed on the side-lower of third time aseptic water washing platform.The filter device
It is placed on the side of pulverizer, the centrifuge is placed on the side of filter device.Compact overall structure is placed in ultra-clean work
On platform.
When operation, first first part of test material is placed in first container, is put on alcohol disinfecting platform, setting oscillation
The duration of oscillation of device pours into and opens oscillation after suitable alcohols in time, after oscillation, opens the switch of container, alcohol is discharged
In alcohol recycle bottle.Then first container is put on mercuric chloride disinfection bench, sets the duration of oscillation of oscillator, pour into appropriate liter
Oscillation is opened after mercury in time, after oscillation, the switch of container is opened, mercuric chloride is thrown into mercuric chloride returnable bottle.Again by first
Container is put on first time aseptic water washing platform, sets the duration of oscillation of oscillator, is poured into and is opened vibration after appropriate amounts of sterilized water in time
It swings, after oscillation, opens the switch of container, sterile water is thrown into sterile water returnable bottle.First container is put into again
On secondary aseptic water washing platform, the duration of oscillation of oscillator is set, pours into and opens oscillation after appropriate amounts of sterilized water in time, oscillation terminates
Afterwards, sterile water is thrown into sterile water returnable bottle by the switch for opening container.First container is put into the punching of third time sterile water again
It washes on platform, sets the duration of oscillation of oscillator, pour into and open oscillation after appropriate amounts of sterilized water in time, after oscillation, open container
Switch, sterile water is thrown into sterile water returnable bottle.The test material through 2 disinfections, 3 rinsings is finally poured into crushing
Machine after liquid feeding body culture medium crushes, is transferred to filter device filtering and centrifuge centrifugation, that is, the disinfection work before completing addition culture medium
Make.
When first part of test material completion alcohol disinfecting in first container, mercuric chloride disinfection is transferred to from alcohol disinfecting platform
After platform, second part of test material can be put into second container, start the initial sterile journey of new material in alcohol disinfecting platform
Sequence, so recycles, and the device of the utility model can handle the sterilization operation of 4-5 parts of test materials simultaneously.
The utility model has the advantages that: (1) mechanized operation instead of manual operations reduce labor intensity, improves
Working efficiency, oscillator oscillation are more thorough in place than hand shaken disinfection, rinsing.(2) multi-level step bracket is small in size, accounts for
Small with space, convenient for sterile working on superclean bench, and the positioning step of 2 disinfections, 3 rinsings is recyclable continuous in an orderly manner
Operation, entire disinfection process is all linked with one another, improves work efficiency, and greatly reduces pollution probability.
The utility model can also be used in the sterilization process of other tissue cultures.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model.
In figure: 1, bracket, 2, oscillator, 3, tool exit vessel, 4, tool switch pipeline, 5, pulverizer, 6, filter device, 7,
Centrifuge.
Specific embodiment
Embodiment 1
As shown in Figure 1, the quick sterilizing operation device of the Isolated Microspore in Chinese Cabbage culture of the utility model, including bracket 1,
Oscillator 2, container 3, pipeline 4, pulverizer 5, filter device 6, centrifuge 7.Bracket 1 is stepped scaffold, and five ranks are arranged altogether
Ladder is alcohol disinfecting platform 8, mercuric chloride disinfection bench 9, first time aseptic water washing platform 10, second of aseptic water washing platform 11, respectively
Aseptic water washing platform 12 three times, the height of each ladder are greater than the height of oscillator 2 plus container 3, and the width of each ladder is vibration
1.5 times for swinging 2 width of device.Oscillator 2 is mini-vibrator, has the function of oscillation frequency adjustable and time.Container 3 is above to open
The container of mouth, lower part side tool liquid outlet, container diameter are less than or equal to the diameter of oscillator 2.Pipeline 4 be 90 degree of bendings, one
End connection container 3 exports, and switch 13 is equipped among pipeline.Pulverizer 5 is placed on the side-lower of third time aseptic water washing platform 12.
Filter device 6 is placed on the side of pulverizer 5, and centrifuge 7 is placed on the side of filter device 6.Compact overall structure is placed in super
On net workbench.
When operation, first first part of test material is placed in first container 3, is put on alcohol disinfecting platform 8, setting vibration
The duration of oscillation for swinging device 2 is 30s, pours into and opens oscillation after suitable alcohols in time, after oscillation, opens the switch 13 of container 3,
Alcohol is thrown into alcohol recycle bottle.Then first container 3 is put on mercuric chloride disinfection bench 9, when setting the oscillation of oscillator 2
Between be 8min, pour into after appropriate mercuric chloride and to open oscillation in time, after oscillation, open the switch 13 of container 3, mercuric chloride is thrown into
Mercuric chloride returnable bottle.First container 3 is put on first time aseptic water washing platform 10 again, set the duration of oscillation of oscillator 2 as
5min is poured into and is opened oscillation after appropriate amounts of sterilized water in time, after oscillation, opens the switch 13 of container 3, sterile water is thrown into
Sterile water returnable bottle.First container 3 is put on second of aseptic water washing platform 11 again, sets the duration of oscillation of oscillator 2
It for 5min, pours into and opens oscillation after appropriate amounts of sterilized water in time, after oscillation, open the switch 13 of container 3, sterile water is discharged
In sterile water returnable bottle.First container 3 is put on third time aseptic water washing platform 12 again, when setting the oscillation of oscillator 2
Between be 5min, pour into after appropriate amounts of sterilized water and to open oscillation in time, after oscillation, open the switch 13 of container 3, sterile water is arranged
It is put in sterile water returnable bottle.The test material through 2 disinfections, 3 rinsings is finally poured into pulverizer 5, liquid feeding body medium powder
After broken, be transferred to the filtering of filter device 6 and centrifuge 7 is centrifuged, that is, the disinfection before completing addition culture medium.
When first part of test material completion alcohol disinfecting in first container, mercuric chloride disinfection is transferred to from alcohol disinfecting platform
After platform, second part of test material can be put into second container, start the initial sterile journey of new material in alcohol disinfecting platform
Sequence, so recycles, and the device of the utility model can handle the sterilization operation of 4-5 parts of test materials simultaneously.
Claims (3)
1. a kind of quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture, which is characterized in that quick chlorination equipment includes bracket, vibration
Device, container, pipeline, pulverizer, filter device, centrifuge are swung, the bracket is stepped scaffold, five ladders are set, point
It is not alcohol disinfecting platform, mercuric chloride disinfection bench, first time aseptic water washing platform, second of aseptic water washing platform, third time sterile water
Scouring table, the oscillator are mini-vibrator, and the container is the container of upper opening, lower part side tool liquid outlet, institute
The pipeline stated is 90 degree of bendings, and one end connects container outlet, is equipped with switch among pipeline, the pulverizer is placed on third
The side-lower of secondary aseptic water washing platform, the filter device are placed on pulverizer side, and the centrifuge is placed on filtering
Device side.
2. the quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture according to claim 1, which is characterized in that the ladder
Height be greater than oscillator and add the height of container, the width of ladder is 1.5 times of oscillator width.
3. the quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture according to claim 1, which is characterized in that the appearance
Device diameter is less than or equal to the diameter of oscillator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820757119.7U CN208809143U (en) | 2018-05-21 | 2018-05-21 | The quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820757119.7U CN208809143U (en) | 2018-05-21 | 2018-05-21 | The quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208809143U true CN208809143U (en) | 2019-05-03 |
Family
ID=66269004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820757119.7U Expired - Fee Related CN208809143U (en) | 2018-05-21 | 2018-05-21 | The quick chlorination equipment of Isolated Microspore in Chinese Cabbage culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208809143U (en) |
-
2018
- 2018-05-21 CN CN201820757119.7U patent/CN208809143U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190503 Termination date: 20200521 |