CN208780904U - Microscope is peeped in a kind of light field fluorescence submicron order - Google Patents
Microscope is peeped in a kind of light field fluorescence submicron order Download PDFInfo
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- CN208780904U CN208780904U CN201821024438.3U CN201821024438U CN208780904U CN 208780904 U CN208780904 U CN 208780904U CN 201821024438 U CN201821024438 U CN 201821024438U CN 208780904 U CN208780904 U CN 208780904U
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- microscope
- endoscope
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- cell
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- 210000004556 brain Anatomy 0.000 claims abstract description 16
- 210000004027 cell Anatomy 0.000 claims abstract description 14
- 208000008918 voyeurism Diseases 0.000 claims abstract description 9
- 239000000835 fiber Substances 0.000 claims abstract description 8
- 230000033001 locomotion Effects 0.000 claims abstract description 7
- 239000013307 optical fiber Substances 0.000 claims abstract description 7
- 210000003520 dendritic spine Anatomy 0.000 claims abstract description 6
- 210000001320 hippocampus Anatomy 0.000 claims abstract description 6
- 230000003287 optical effect Effects 0.000 claims abstract description 6
- 238000003384 imaging method Methods 0.000 claims abstract description 5
- 230000000926 neurological effect Effects 0.000 claims abstract description 5
- 208000005623 Carcinogenesis Diseases 0.000 claims abstract description 4
- 230000036952 cancer formation Effects 0.000 claims abstract description 4
- 231100000504 carcinogenesis Toxicity 0.000 claims abstract description 4
- 210000004046 cell surface extension Anatomy 0.000 claims abstract description 4
- 206010028980 Neoplasm Diseases 0.000 claims description 15
- 201000011510 cancer Diseases 0.000 claims description 11
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 238000005070 sampling Methods 0.000 claims 1
- 210000002569 neuron Anatomy 0.000 abstract description 2
- 230000029052 metamorphosis Effects 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 description 8
- 238000001574 biopsy Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 210000005036 nerve Anatomy 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 238000001839 endoscopy Methods 0.000 description 2
- 244000144985 peep Species 0.000 description 2
- 230000002980 postoperative effect Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 206010018341 Gliosis Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000004221 Multiple Trauma Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000007177 brain activity Effects 0.000 description 1
- 210000003710 cerebral cortex Anatomy 0.000 description 1
- 210000003618 cortical neuron Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007387 gliosis Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000000274 microglia Anatomy 0.000 description 1
- 238000002324 minimally invasive surgery Methods 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to peeping microscope in a kind of light field fluorescence submicron order.It includes the components such as miniature object lens, optical fiber, fiber adapter, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, video camera and adjustment structure, CMOS camera, PC control terminal and the control box of high-NA.Cell can be amplified 1000 times or more by the endoscope microlens of this submicron order.Submicron resolution, it can be seen that the metamorphosis of subcellular level can effectively observe the heteromorphism of tissue such as the direct variation of born of the same parents' device or karyomorphism；Advantage is real time imagery, can directly differentiate position and the stage of carcinogenesis in body.Europe and domestic key lab observe for living body depth brain neurological motion, somatoscopy animal brain deep layer, the imaging of brain deep layer hippocampus neuron, and the functional area dendritic spines (less than 1 micron) of cell surface protrusion are high-visible.
Description
Technical field
The utility model relates to peeping microscope in a kind of light field fluorescence submicron order.It is widely used in medicine and nerve
Field of scientific study, more particularly to peeping microscope in a kind of light field fluorescence submicron order.Microscope is peeped in this, can be understood
The boundary of cancerous issue and normal tissue is distinguished on ground, accomplishes precisely to perform the operation, and can with maximum magnitude be retained the tissue of health, is conducive to
Post-operative recovery；It can effectively improve cancer diagnosis rate and cure rate, can be widely applied to the observation of living body depth brain neurological motion, push away
Dynamic brain research.
Background technique
Endoscopy is the important diagnosis and treatment method of early detection cancer, but due to the limit of conventional endoscope imaging multiplying power
System will finally be made a definite diagnosis toward contact by living body puncture.Traditional pathology is long from biopsy to diagnosis out time, need through
Biopsy, freezing sample then slice are gone through, handles and be fixed to film-making on glass slide, HE contaminates piece, and pathologist sees piece in microscope
The process of diagnosis lasts long；And since pathologist work fatigue is possible to fail to pinpoint a disease in diagnosis during diagnosing, biopsy is sampled such as
Fruit cannot accurately get the tissue of lesions position, the limitation etc. of pathological section, and film-making and dye tablet quality etc. can all influence to diagnose
Conclusion.Present cancer is commonplace, and early discovery, early treatment major part cancer can cure.
Biopsy synchronous cannot be carried out with diagnosis and operation under traditional pathology mode, need to sample when biopsy, sample is sent
Inspection, pathological diagnosis, then perform the operation；Alimentary tumor patient needs to undergo an endoscopy and a surgical procedure, intermediate
Several days waiting time are needed, cannot be handled in time.
The utility model is that microscope is peeped in a submicron order, and accuracy of observation reaches 0.4 micron, does not need puncture and takes
Sample, so that it may realize that the living body of cancer cell precisely detects.Conventional endoscope is broken through, traditional pathology mode is needed not move through, is examined
It can look into and diagnose in physical examination when looking into, while be clearly apparent the tissue morphology of tumour, accurate judgement tumor boundaries, effective nondominant hand
Art doctor precisely judges and tumor resection tissue, avoids the multiple wound to patient.
Summary of the invention
As shown in Figure 1, peeping microscope in one kind is matched with endoscope.More particularly to a kind of light field fluorescence submicron order
Inside peep microscope.It includes object lens, optical fiber, fiber adapter, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, takes the photograph
The components such as camera and adjustment structure, CMOS camera, PC control terminal and control box.
Diameter only has in 2.6 millimeters of endoscope lens, containing 7 groups of hand-made eyeglasses, passes through wireless connection, so that it may
To realize the accurate movement of camera lens in controlling terminal.In general, the enlargement ratio of conventional endoscope is about at 5 times or so,
And the endoscope microlens of this submicron order, then cell can be amplified 1000 times or more.Submicron resolution can be seen
To the variation of cell born of the same parents' device level, it is exactly the direct variation of subcellular form, can effectively observes the heteromorphism of tissue, advantage
It is real time imagery, can directly differentiates position and the stage of carcinogenesis in body.Europe and domestic key lab are for living
The observation of body depth brain neurological motion, somatoscopy animal brain cortex, the imaging of brain deep layer hippocampus, the function of cell surface protrusion
Energy region dendritic spines (less than 1 micron) are high-visible.
Detailed description of the invention
Attached drawing 1 is that the utility model constitutes component drawings；
Attached drawing 2 is the Cerebral Cortex and hippocampus nerve dendritic spines that the utility model is observed；
Attached drawing 3 is the mouse brain depth cortical neuron and hippocampus neuron that the utility model is observed
Attached drawing 4 is that (red is brain gliosis cytoma to the cancerous tissue form abnormity figure observed of the utility model, green is
Microglia)；
1. objective object lens；2. fiber adapter fiber adapter；3. fiber optical fiber；4. fiber
Adapter fiber adapter；5. fine Piezo Z axis piezoelectricity Z axis；6. manual drive corset Z axis
The manual Z axis adjusting knob of focus knob；7. fluore cube flourescent sheet group；8. epi illuminater fluorescence, which is fallen, penetrates photograph
Bright device；9. LED light source LED light source；10. camera adapter camera adapter；11. CMOS
Camera CMOS camera；12. Control Box controls box；13. Main unit master controller.
Specific embodiment
The dendritic spines (less than 1 micron) of Neuroscience Research experiment, the imaging of brain deep layer hippocampus, cell surface protrusion are clear
It is clear visible.The transmitting of dendritic spines responsible nerve signal is the region that the brain activities such as learning and memory emotion occur；Because this is practical new
It is high that the resolution of microscope is peeped in type, and can widely be adopted by Europe and domestic key lab with real time imagery
Purchase uses, and observes for living body depth brain neurological motion, somatoscopy animal brain deep layer.
The utility model can be very big for the promotion of neurology research, will also generate huge promotion for medical development
Effect.This diameter only has in 2.6 millimeters of endoscope lens, containing 7 groups of hand-made eyeglasses, by being wirelessly connected, just
The accurate movement of camera lens can be realized in controlling terminal.In general, the enlargement ratio of conventional endoscope is about on 5 times of left sides
Cell then can be amplified 1000 times or more by the right side, and the endoscope microlens of this submicron order.Submicron resolution, can
To see the variation of cell level, it is exactly the direct variation of the form of cell, can effectively observes the heteromorphism of tissue, advantage exists
In real time imagery, position and the stage of carcinogenesis can be directly differentiated in body.In addition to being used for cancer diagnosis, this submicron order
Endoscope may also help in the accurate excision that doctor realizes cancer surgeries, and in cancer operation, doctor's most headache is exactly
Determine the boundary of cancer cell and normal cell, if cancerous issue excision is imperfect, remaining cancer cell also will continue to grow, and
Excision excessively, then can cause huge harm human body, be unfavorable for post-operative recovery.And it is this in peep microscope, can understand area
The boundary of other cancerous issue and normal tissue accomplishes precisely to perform the operation.
Microscope shape very little is peeped in the utility model, precisely, doctor is when in use by enteron aisle mirror for optical fiber transmission
Biopsy channel does not need in addition to increase affiliated facility, will not damage to patient, can be widely applied to Minimally Invasive Surgery, carries out
It observes and performs the operation to be imaged in body and use.
Claims (6)
1. matched with endoscope interior microscope is peeped a kind of, it is characterised in that microscope is peeped in a kind of light field fluorescence submicron order,
Including object lens, optical fiber, fiber adapter, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, video camera and adjustment knot
Structure, CMOS camera, PC control terminal and control box component；Controlling box includes software central control system；Object lens, optical fiber, optical fiber are suitable
It is connected between orchestration by mechanical engagement, manual Z axis adjusting knob, fluorescent optical filter group, LED light source, video camera and adjustment knot
Structure is connected through a screw thread；Fluorescent optical filter group uses TUBE application specific architecture, optical coating eyeglass is combined use, Z axis can
Separate manual adjusts focus function.
2. peeping microscope in one kind according to claim 1 is matched with endoscope, it is characterised in that diameter only has 2.6 millis
In the endoscope lens of rice, containing the hand-made eyeglass of multiple groups, cell can be amplified 1000 times or more.
3. peeping microscope in one kind according to claim 2 is matched with endoscope, it is characterised in that peeped in submicron order
Microscope, accuracy of observation reach 0.8 micron, do not need to puncture sampling, so that it may realize that the living body of cancer cell precisely detects.
4. peeping microscope in one kind according to claim 3 is matched with endoscope, it is characterised in that submicron resolution,
It can be seen that the variation of cell level, is exactly the direct variation of the form of cell, can effectively observe the heteromorphism of tissue.
5. peeping microscope in one kind according to claim 4 is matched with endoscope, it is characterised in that real time imagery, it can be with
Position and the stage of carcinogenesis are directly differentiated in body.
6. peeping microscope in one kind according to claim 4 is matched with endoscope, it is characterised in that be used for living body depth
Brain neurological motion observation, somatoscopy animal brain deep layer, the imaging of brain deep layer hippocampus, the dendritic spines of cell surface protrusion are clear
It is clear visible.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821024438.3U CN208780904U (en) | 2018-07-02 | 2018-07-02 | Microscope is peeped in a kind of light field fluorescence submicron order |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821024438.3U CN208780904U (en) | 2018-07-02 | 2018-07-02 | Microscope is peeped in a kind of light field fluorescence submicron order |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208780904U true CN208780904U (en) | 2019-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821024438.3U Active CN208780904U (en) | 2018-07-02 | 2018-07-02 | Microscope is peeped in a kind of light field fluorescence submicron order |
Country Status (1)
| Country | Link |
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| CN (1) | CN208780904U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114577125A (en) * | 2022-04-08 | 2022-06-03 | 上海树突精密仪器有限公司 | Non-contact optical lens center thickness measuring method and measuring device |
-
2018
- 2018-07-02 CN CN201821024438.3U patent/CN208780904U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114577125A (en) * | 2022-04-08 | 2022-06-03 | 上海树突精密仪器有限公司 | Non-contact optical lens center thickness measuring method and measuring device |
| CN114577125B (en) * | 2022-04-08 | 2024-01-19 | 上海树突精密仪器有限公司 | Non-contact optical lens center thickness measuring method and measuring device |
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| GR01 | Patent grant | ||
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| CP03 | Change of name, title or address |
Address after: Building A1, 13th Floor, Zhong'an Chuanggu Science and Technology Park, No. 900 Wangjiang West Road, High tech Zone, Hefei City, Anhui Province, 230088 Patentee after: Anhui Dendritic Medical Equipment Co.,Ltd. Country or region after: China Address before: 9th Floor, No. 250 Jiangchang 3rd Road, Jing'an District, Shanghai 200040 Patentee before: SHANGHAI SHUTU PRECISION INSTRUMENT CO.,LTD. Country or region before: China |
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| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: B007, Building F8, Phase II of Zhong'an Chuanggu Science and Technology Park, No. 920 Wangjiang West Road, High tech Zone, Hefei City, Anhui Province, 230094 Patentee after: Anhui Dendritic Optical Technology Co.,Ltd. Country or region after: China Address before: Building A1, 13th Floor, Zhong'an Chuanggu Science and Technology Park, No. 900 Wangjiang West Road, High tech Zone, Hefei City, Anhui Province, 230088 Patentee before: Anhui Dendritic Medical Equipment Co.,Ltd. Country or region before: China |
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| CP03 | Change of name, title or address |