CN208250337U - Novel enclosed T cell culture apparatus - Google Patents
Novel enclosed T cell culture apparatus Download PDFInfo
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- CN208250337U CN208250337U CN201820502763.XU CN201820502763U CN208250337U CN 208250337 U CN208250337 U CN 208250337U CN 201820502763 U CN201820502763 U CN 201820502763U CN 208250337 U CN208250337 U CN 208250337U
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- culture
- cartridge body
- upper cartridge
- pedestal
- culture tank
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Abstract
The utility model provides novel enclosed T cell culture apparatus, including upper cartridge body, pedestal, upper cartridge body and pedestal are fixedly connected, multiple culture spaces are separated by multiple fixed plates in the upper cartridge body, pull connection, liquid changing type culture tank are provided in culture space, electromagnet is provided with above each culture tank, the bottom of each culture tank is all provided with leakage fluid dram, apocenosis passage on leakage fluid dram is spaced apart from each other stretching pedestal, and each culture space is corresponded on upper cartridge body outer surface of cupular part and is provided with supply line and observation device；This culture apparatus is using upper cartridge body and the fixed confined space for constituting culture T cell of pedestal, by the first inlet at the top of upper cartridge body, the second inlet and air inlet carry out gas be filled with and the replacement of culture solution, it avoids being influenced by the external world, the separation of T cell is realized in electromagnet and the cooperation of liquid changing type culture tank using electromagnet to the suction-operated of magnetic bead, and observation window can carry out subsequent observation and analysis.
Description
Technical field
The utility model relates to novel enclosed T cell culture apparatus.
Background technique
T cell is segmented into many types, such as cytotoxic T cell according to function and surface marker, T helper cell, adjust/
Inhibit T cell etc., with the development of biological therapy clinical application, T cell is more and more wider in the research and application of medical field
It is general, and carry out separation, proliferation and the detection of T cell etc. and be often also easy to produce target in transfer process repeatedly for different devices
The loss of cell, and when carrying out multiple groups culture or single group culture, the shadow because of open culture environment, vulnerable to extraneous factor
It rings, and then influences testing result.
Utility model content
In view of this, can be separated, be trained the purpose of the utility model is to provide novel enclosed T cell culture apparatus
Support and observe etc., the interference and mutual cross-infection of extraneous factor are avoided, the loss of T cell is reduced.
In order to solve the above technical problems, the technical solution of the utility model is:
Novel enclosed T cell culture apparatus, including upper cartridge body, pedestal, upper cartridge body and pedestal are fixedly connected, the upper box
Multiple culture spaces are separated by multiple fixed plates in vivo and fixed plate abuts pedestal when upper cartridge body is fixed with pedestal, and culture is empty
It is interior to be provided with pull connection, liquid changing type culture tank, electromagnet, the bottom of each culture tank are provided with above each culture tank
Portion is all provided with leakage fluid dram, and the apocenosis passage on leakage fluid dram is spaced apart from each other stretching pedestal, and each training is corresponded on upper cartridge body outer surface of cupular part
Feeding space is provided with supply line and observation device, and wherein supply line includes the first inlet, the second inlet and air inlet
Mouthful, observation device includes transparent windows, and drawing and pulling type closure door is provided on transparent windows.
This culture apparatus is using upper cartridge body and the fixed confined space for constituting culture T cell of pedestal, by the top of upper cartridge body
The first inlet, the second inlet and air inlet carry out gas be filled with and the replacement of culture solution, avoid being influenced by the external world, electricity
The separation of T cell is realized in magnet and the cooperation of liquid changing type culture tank using electromagnet to the suction-operated of magnetic bead, and observation window can carry out
Subsequent observation and analysis.
Further, the upper cartridge body includes side eaves portion, and base side wall is that second level is stepped, and the first terrace passes through with eaves portion
Bolt is fixed, easy to connect, avoids the influence of extraneous factor.
Further, the position setting that upper cartridge body fixed plate is corresponded in the slot that first terrace of pedestal surrounds is fluted, leads to
The independence that fixed plate insertion groove realizes each culture space is crossed, cross-infection is avoided.
Further, the fixed plate or upper cartridge body side wall of the culture tank and composition culture space form drawing and pulling type by sliding slot
Connection, culture trench bottom is prismoid shape, and bottom is provided with drawing type filter screen, is provided with drawing and pulling type valve below strainer,
This culture tank drawing and pulling type structure can be replaced at any time, and the separation of target cell, and strainer, valve are realized in strainer, valve and electromagnet cooperation
Replaceable, the expanded service life of door.
Further, the pedestal is fixedly connected with upper cartridge body, and base side wall corresponds to the position setting of culture tank bottom valve
There is the square hole stretched out for valve, the insertion and extraction of valve can be carried out by square hole, realizes the connection of culture tank and leakage fluid dram,
Carry out the replacement of culture solution.
The utility model has the beneficial effects that this culture apparatus carries out separation, culture and the sight of T cell using closed environment
It examines, the interference and mutual cross-infection for avoiding extraneous factor without carrying out the transfer in place reduce T cell
Loss.
Detailed description of the invention
Fig. 1: the structure chart of the utility model；
Fig. 2: the upper cartridge body overlooking structure figure of the utility model；
Fig. 3: the culture tank structure chart of the utility model；
Fig. 4: the pedestal side view of the utility model；
In figure: 1, pedestal；1.1, the first terrace；1.2, leakage fluid dram；1.3, apocenosis passage；1.4, groove；1.5, square hole；2,
Upper cartridge body；2.1, fixed plate；2.2, sliding slot；2.3, eaves portion；2.4, it seals up a door；2.5, observation window；2.6, the first inlet；2.7, into
Port；2.8, the second inlet；3, culture tank；3.1, strainer；3.2, valve；4, electromagnet.
Specific embodiment
Below in conjunction with attached drawing, specific embodiment of the present utility model is described in further detail, so that the utility model skill
Art scheme is more readily understood and grasps.
Fig. 1 illustrates the specific structure of novel enclosed T cell culture apparatus, including upper cartridge body 2, pedestal 1, upper cartridge body 2
And pedestal 1 is fixedly connected, upper cartridge body 2 and pedestal 1 use polypropylene injection molding, by reserved notch carry out closure door 2.4 and
The installation of observation window 2.5, the upper cartridge body 2 is interior to be separated into multiple culture spaces and fixed plate 2.1 upper by multiple fixed plates 2.1
Box body 2 abuts pedestal 1 when fixing with pedestal 1, it is preferable that the position setting groove 1.4 of fixed plate 2.1, engaging are corresponded on pedestal 1
Fixed plate 2.1 realizes the independence of each culture space, avoids cross-infection while avoiding the influence of extraneous factor, in culture space
Be provided with pull connection, liquid changing type culture tank 3, it is preferable that the culture tank 3 and the fixed plate 2.1 for constituting culture space or on
2 side wall of box body forms drawing and pulling type connection by sliding slot 2.2, this structure is conveniently replaceable culture tank 3, extends the validity period of the present apparatus
Limit, as shown in figure 3,3 bottom of culture tank is prismoid shape, and bottom is provided with drawing type filter screen 3.1, sets below strainer 3.1
It is equipped with drawing and pulling type valve 3.2,3 drawing and pulling type structure of this culture tank can be replaced at any time, and strainer 3.1, valve 3.2 and electromagnet 4 cooperate
Realize the separation of target cell, and strainer 3.1, valve 3.2 are replaceable, expanded service life, as shown in Figure 1, each culture tank 3
Top is provided with electromagnet 4, and the bottom of each culture tank 3 is all provided with leakage fluid dram 1.2, and the apocenosis passage 1.3 on leakage fluid dram 1.2 is mutual
Pedestal 1 is stretched out at interval, and the absorption of magnetic bead is carried out by the cooperation of electromagnet 4 and culture tank 3, realizes the separation of cell, while can
The replacement for carrying out culture solution, as shown in Fig. 2, corresponding to each culture space on 2 outer surface of cupular part of upper cartridge body is provided with supply pipe
Road and observation device, wherein supply line includes the first inlet 2.6, the second inlet 2.8 and air inlet 2.7, wherein first
Inlet 2.6, the second inlet 2.8, which can transport, cultivates required liquid, and air inlet 2.7 carries out the conveying of gas, observes device packet
Include transparent windows 2.5, it is preferable that observation window 2.5 is done using glass, after the situation and progress inside observation culture apparatus
Continuous analysis etc. operates, and drawing and pulling type closure door 2.4 is provided on transparent windows 2.5, can completely cut off illumination at any time.
As a further improvement, as shown in Figure 1, the upper cartridge body 2 includes side eaves portion 2.3,1 side wall of pedestal is second level
Step type, the first terrace 1.1 is bolted with eaves portion 2.3, easy to connect, avoids the influence of extraneous factor.
As a further improvement, as shown in Figure 1, corresponding to upper cartridge body 2 in the slot that 1 first terrace 1.1 of the pedestal surrounds
The position setting fluted 1.4 of fixed plate 2.1, is embedded in the independence that groove 1.4 realizes each culture space by fixed plate 2.1, keeps away
Exempt from cross-infection.
As a further improvement, as shown in figure 4, the pedestal 1 is fixedly connected with upper cartridge body 2, the corresponding training of 1 side wall of pedestal
The position for supporting 3 bottom valve 3.2 of slot is provided with the square hole 1.5 stretched out for valve 3.2, can carry out valve 3.2 by square hole 1.5
Insertion and extraction, realize culture tank 3 and leakage fluid dram 1.2 connection, carry out the replacement of culture solution.
This culture apparatus carries out separation, culture and the observation of T cell using closed environment, avoids the interference of extraneous factor
And mutual cross-infection reduces the loss of T cell without carrying out the transfer in place.
Certainly, the above is only the specific application examples of the utility model, do not constitute and appoint to the protection scope of the utility model
What is limited.Any technical scheme formed by adopting equivalent transformation or equivalent replacement, all falls within the utility model rights protection model
Within enclosing.
Claims (5)
1. novel enclosed T cell culture apparatus, which is characterized in that including upper cartridge body, pedestal, upper cartridge body and the fixed company of pedestal
It connects, is separated into multiple culture spaces by multiple fixed plates in the upper cartridge body and fixed plate is abutted when upper cartridge body is fixed with pedestal
Pedestal is provided with pull connection, liquid changing type culture tank in culture space, is provided with electromagnet above each culture tank, often
The bottom of a culture tank is all provided with leakage fluid dram, and the apocenosis passage on leakage fluid dram is spaced apart from each other stretching pedestal, upper cartridge body outer surface of cupular part
The upper each culture space of correspondence is provided with supply line and observation device, and wherein supply line includes the first inlet, second
Inlet and air inlet, observation device includes transparent windows, and drawing and pulling type closure door is provided on transparent windows.
2. novel enclosed T cell culture apparatus according to claim 1, which is characterized in that the upper cartridge body includes week
Side eaves portion, base side wall are that second level is stepped, and the first terrace is bolted with eaves portion.
3. novel enclosed T cell culture apparatus according to claim 2, which is characterized in that first terrace of pedestal
The position setting that upper cartridge body fixed plate is corresponded in the slot surrounded is fluted.
4. novel enclosed T cell culture apparatus according to claim 1, which is characterized in that the culture tank and composition
The fixed plate or upper cartridge body side wall of culture space form drawing and pulling type by sliding slot and connect, and culture trench bottom is prismoid shape, and
Bottom is provided with drawing type filter screen, is provided with drawing and pulling type valve below strainer.
5. novel enclosed T cell culture apparatus according to claim 4, which is characterized in that the pedestal and upper cartridge body
It is fixedly connected, the position that base side wall corresponds to culture tank bottom valve is provided with the square hole stretched out for valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820502763.XU CN208250337U (en) | 2018-04-10 | 2018-04-10 | Novel enclosed T cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820502763.XU CN208250337U (en) | 2018-04-10 | 2018-04-10 | Novel enclosed T cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208250337U true CN208250337U (en) | 2018-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820502763.XU Active CN208250337U (en) | 2018-04-10 | 2018-04-10 | Novel enclosed T cell culture apparatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN208250337U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021219075A1 (en) * | 2020-04-29 | 2021-11-04 | Medical And Pharmaceutical Industry Technology And Development Center | Cell culture device |
-
2018
- 2018-04-10 CN CN201820502763.XU patent/CN208250337U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021219075A1 (en) * | 2020-04-29 | 2021-11-04 | Medical And Pharmaceutical Industry Technology And Development Center | Cell culture device |
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